TURFGRASS DISEASE RESEARCH REPORT J. M. Vargas, Jr. and R. Detweiler Department of Botany and Plant Pathology Michigan State University
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1 I TURFGRASS DISEASE RESEARCH REPORT 1975 J. M. Vrgs, Jr. nd R. Detweiler Deprtment of Botny nd Plnt Pthology Michign Stte University 1. Snow Mold The 1975 snow mold fungicide evlution trils were conducted t the Boyne Highlnd Resort, Hror Springs, Michign on "Penncross" creeping entgrss mowed t 1/2 inch. No fungicides were pplied to the test re during the growing seson. The wettle powder (WP) nd flowles (F) were pplied with CO 2 smll plot spryer nd the grnulr fungicide with 3 foot Scotts spreder. The test ws divided into two studies ecuse of the lrge size. The plots were 6 ft. x 6 ft. nd the tretments were replicted 3 times in rndom lock design for ech study. Study A The resul ts of Study A cn e seen in Tle 1. They show tht prcti cll y ll the snow mold present ws Typhul light with only little Fusrium ptch s indicted. In ddition to the old stndys, Clo grn nd Clo clorj Scotts F + F Dow 281, MF 582, Terrclor nd Acti-dione RZ ll rnked high. With the exception of Clo grn, cl0 clor, nd Dow 281, ~ll these mterils contin PCNB (MF 582 nd Acti-dione RZ) or were penb (Terrclor nd Scotts F + F with the F + f lso contining fertilizer). On n ppernce sis, the Scotts F + F ws the est, minly due to the effect of the fertilizer on grss color nd growth. This is the second seson in which good results hve een otined with the PCNB fungicides ginst Typhul light. Study B Study B hd oth Typhul light nd Fusrium ptch present. They re listed together on Tle 2 s % snow mold nd roken down in Tle 2 to % Typhul light nd Tle 2 to % Fusrium ptch. The results in Tle 2, 2, 2, show tht wheres Fusrium ptch ws present in this study, the predominnt snow mold ws Typhul light. Clery's 4222 nd 4223; Dconil 2787 t the 1/4 pt., 1/2 pt., nd 1 pt. lone nd in comintion with Exhlt 800 or Tersn SP 6 oz; Tersn SP 9 oz.; Scotts Fungi c fde t the 2 X rte; ll gve signi f icnt cont ro I compred to the unt reted check, Tle 2. When the results re fctored out in tles 2 nd 2, it cn e seen tht the mjority of the snow mold present in the Dconil 2787 nd Clery's 4222 nd 4223 plots ws due to Fusrium ptch, wheres the mjority of the snow mold present in the Tersn SP nd Scotts Fungicide plots t the high rte ws due to Typhul light. The untreted control hd 43% Typhul light nd 17% Fusrium ptch. 7
2 Tle 1. Percent Typhul light on Penncross creeping entgrss, except where otherwise indicted, t the Boyne Highlnd Resort, Hror Springs, MI Chemicl & Rte/l0DO s9 ft Clo-grn Scotts F+F Clo-elor Dow 281 MF 582 Terrclor MF 582 Aeti-dione RZ Terrelor MF 594 MF 594 MF 582 Dow 342 Dow 263 MF 594 Mi Io rqrrite IBDU El 222 Check Ure 8 1s 1 x 4 oz 8 oz 9 oz 8 oz 12 oz 8 oz 4 oz 1s 7 1 s 6 oz 8 oz 8 oz 5 1s 2 1s 2 1s 8 oz 2 1s I o Percent o infected I o 5 o re - represents the following % Fusrium Ptch included in the numer: AV o 2, c c c c c c e c c d d d d d e e e e MF ~~; Dow %; Dow nd 2% respectively; Clo Clor ~~; MF 594-5%. - Tretments follow y the sme level re not significntly different t the ~~ 1eve 1. 8
3 Tle 2. Percent Typhul light nd Fusrium light on Penncross creeping entgrss t the Boyne Highlnds Resort, Hror spring, HI Chemicl nd Rte/lOOO sq. ft. Percent Infected re I I AVE Clery's s Clery's s Dconil /2 pt Exhlt 8 oz Dconil pt + Exhlt 8 oz Dconil /2 pt Tersn sp. 6 oz Dconil /2 pt Dconi /4 pt + Tersn sp. 6 oz Dconil pt + Tersn sp. 6 oz Dconil pt Tersn SP 9 oz Dconil /4 pt Scotts Fungicide 2 x Dconil /4 pt & Exh1t 8 oz Clery's s c Tersn SP 6 oz c I Scotts Fungicide 1 x c Check c d Exhlt 8 oz d - Tretments followed y the sme letter not significntly different t the ~h level 9
4 Tle 2. Percent infection of study B due to Typhul light. Chemicl nd Rte/IOOO sq. ft. Percent infected re I I AVE Dconil pt Dconi /4 pt+exh 1t 80z Dconi /2 pt+exhlt 80z Dconil pt+exhlt " Dconi /4 pt+tersn SP 6 oz. Dconi pt+tersn SP oz. Dconil /2 pt Clerys s Dconil /2 pt+tersn SP 6 oz. Clerys s Dconil /4 pt Ter sn SP 9 oz Scotts Fungi cide 2X c Tersn SP 6 oz c Clerys t s c Scotts Fungicide IX c d Check c d Exhlt 8 oz d - Tretments followed y the sme letter not significntly different t the SO~ 1eve 1.
5 Tle s, Percent infection of study B due to Fusrium Ptch. Chemicl nd Rte/l000 ft. Percent infected re s9 I I AV Clerys s Clerys s Tersn SP 9 oz Scotts Fungicide 2X Scotts Fungicide lx Dconil /2 pt+tersn SP 6 oz. Dconi /2 pt+exh 1t 80z Dconi pt+exh1t 80z Dconi /2 pt Dconi /4 pt 0 20 c Exhlt c C1erys s c Dconi /4 pt+tersn SP c 6 oz. Dconil pt+ter. SP 6 oz c Dconi pt c Check c Tersn SP 6 oz c Dconil /4 pt+exhlt c 8 oz - Tretments followed y the sme letter not significntly different from ech other t the 5% 1eve 1 11
6 2. Helminthosporium lef Spot Study The Helminthosporium lef spot study ws conducted t the M.S.U. Soils Frm on Prk Kentucky Bluegrss mintined t 2 inch height of cut. The plots were 6 x 6 ft. nd replicted 3 times in rndom lock design. The liquid pplictions were mde with 002 hnd held spryer nd the dry pplictions were mde with Scotts 3 ft. spreder. The tretments were pplied on My 8, 15, 30 nd June 12 except for Scotts F + F which ws pplied only once on My 8 nd Spectro nd Clery's 4223 which were pplied twice on My 30 nd June 12. The redings were tken on June 19. The plots were rted on 0-9 scle with 0 representing no spots present on the lef lde nd 9 representi ng 90% or more of the 1ef l de surfce covered with spots. The results in Tle' show tht most of the fungicides t one or more rtes gve significnt control when compred to the untreted check. The noticele exceptions eing EL-222, Clery's 4223, nd Fungo. Those rnking highest in the study (giving the est control) were RP 26019, z., Dyrene 4, 5, 6, 8 oz., Dconil , 6, 8 oz., Dconil oz., plus Exhlt pt/loo gl nd Acti-dione RZ, 4 oz. Tle 1. Helminthosporium lef Spot Fungicide Trils R tlngs 2 Rte of 2 1 Tretment 00 ft I I Averge RP oz RP oz Dyrene 4 oz Dyrene 5 oz c Dyrene 8 oz c d Dyrene 6 oz 'i..7 c d Dconil oz c d Dcorri oz. Exhlt 800 pt/l00 gl c d Dconi oz. Exhlt 800 pt/l00 gl c d Dcorri oz c d e Dconil oz c d e Ac t ivdione RZ 4 oz c d e Dconi oz c d e f DPX oz c d e f g MF oz c d e f g Scotts F+F Ix d e f g h Bromosn 6 oz d e f g h 12
7 Tle 1 (cont' d) Tersn lsr 4.5 oz e f 9 h i DPX oz f 9 h i j Terrclor 4 oz f 9 h i j MF oz f g h i j Spectro 4 oz g h i j Terrclor 2 oz g h i j Actidione-Thirm 2 oz h ; j Actidione-Thirm 4 oz i j Clery's oz i j El oz i j Fungo 1.8 oz i j Tersn lsr 3 oz i j Actidione-RZ 2 oz j Bromosn 3 oz j k Clery's oz j k EL oz j k Spect ro 2 oz j k Fungo 1.2 oz k Exhlt 800 pt/loo gl k Check Tretments followed y the sme letter re not significntly different from ech other t the ~h level. 2. l-lest disese; 9-most disese. 3. Sclerotini Dollr Spot Study The regulr Sclerotini dollr spot study ws conducted on the M.S.U. Crop Science Frm. The plots were 3 x 6 ft. nd the tretments were replicted 3 times in rndom lock design. All tretments gve significnt control compred to the untreted control. The tretments rnking t the top cn e seen in Tle 2. 13
8 c o... ~ ~ u 0 :::J "CU) 0) t- t- ~- eo c- O) 0 u"c t-~ 0) 0 CL. N~-N~"''''~~O~~N~~N--O~'''~OOONOO~~ ~~~N-O~~OO"'~~~NN--~~~~~OO~O~~~ OOOOOOcx)OOCX)~~~~~~~~~~~"''''~~~~~~~N''' I -'d\ ~ ot- ~~OO-NNcx)~"'N~N~~N~~"'N-~~cx)~~~~- _NN_N_~N_~ N~~~N~~"'N~OO~~~ ~~~OO~O~~O~O~~~O~~~O~O"'O~~~O ~"'~~~~"'-OO~~~"'OOO~-OOO~N~NOO"'--~ -_- --N NN~-~ <o N <, - t- OO ~.8 0) o E t- f-:::j 0 e ~ 0). ~ 01 e... ern..., "C..., 0 O'~ 0-0) ::: > &I) c:( ~... ~ o0- Il) t- ō -c... -e Q) U C/) ~ o o t- ~ eo u ~ ~... ) N N N N N N N N N N--..:t- N'" <, Q) ~ 0::: m 01.. N - N N N N N N N N N N N N N 0 N N N ~~..:t ~- ~CO"'OON-OO~"""""'~CON ~. N 0) :c f- 14
9 4. Benzimidzole resistnt dollr spot study The enzimidzole resistnt dollr spot study ws conducted on the M.S.U. Soil Frm. The tretments in study numer 1 were pplied on 7/8, 7/23, 8/7, 8/19 nd 9/4. The tretments in Study 2 were pplied on 8/7 nd 8/19. All tretments were pplied with hnd held CO spryer. 2 Study 1 The results show none of the fungicides were effective in controlling the enzimidzole resistnt strin of dollr spot Tle 3. Study 2 The study ws red on 8/26. Tle 4 shows Dconil 2787, 6 oz,, R. P oz,, Clo Clor 2 oz., R. P oz., Actidione - Thirm 4 oz., Clo Clor 3 oz., Actidione RZ 2 oz., nd Dyrene 6 oz. rnked t the top of those giving significnt control over the enzimidzole resistnt strin of dollr spot. Clo Clor nd Acti-dione RZ were phytotoxic. It is ovious for complete control shorter tretment intervls will e requi red. Tle 3. Control of enzimidzole resistnt Sclerotini dollr spot. Ave. Numer of s)ots/elot Tretment I Rte I (8/26) (9.9 I (9715) MF oz. 400* 400')~ 400* MF oz. MF oz. Dithne M oz. Dithne M-45 3 oz. Dithne M oz. " " Fungo 50.9 oz. " Fungo oz. " " Fungo oz. " " Check " " " " -;,t: Estimtion - spots too numerous to count. 1 Tretment dtes re 7/8, 7/23, 8/23, 8/19. 15
10 Tle 4. Control of enzimidzole resistnt Sclerotini dollr spot. Numer of spots rte/ 1 Tretment 00 sq.ft. I I Averge Dconi oz R.P oz Clo-Clor 2 oz R. P oz Actidione-Thirm 4 oz Cle-ct or 3 oz Actidione-RZ 2 oz ~ Dyrene 6 oz Actidione-Thirm 2 oz c Dconil oz c Actidione-RZ 1 oz c d Dyrene 3 oz d Tersn 75 3 oz e Tersn oz. 400~'( 400''''' 400~''' 400~''' f Clery's oz. 400~'" 400''''- 400~'" 400~'.. f Check ~t( 400~\- 400~t.. f 1 Tretment followed y the sme letter re not significntly different from ech other t the ~~ level t. ftestimtion - spots too numerous to count. phytotoxic to the turf. 16
11 5. Anthrcnose The Anthrcnose studies were conducted t the Derorn C.C. in Derorn, HI on the firwy which ws primrily Po nnu cut t 1/2 inch height. The plots were 6,x 6 ft. nd replicted in rndom lock design. The liquid pplictions were mde with CO 2 hnd held spryer nd the dry formultion with Scotts 3 ft.' drop spreder.. Study I Tretments for Study I were pplied on 8/6 nd 8/16 except for Tersn oz., Scotts F + F nd Ammonium Nitrte which were only pplied once on August 6. The systemic fungicide Tersn 1991, DPX nd Scotts Fertilizer plus DSB Fungicide were ll drenched in fter ppliction. The plots were evluted on percent thinning due to Anthrcnose. The redings were tken on 9/11. The results in Tle 1 show tht Tersn 1991 t 8 oz., 4 oz., nd 1 oz.; DPX t 8 oz., nd 4 oz.; nd Scott's Fertilizer & DSB Fungicide ll gve significnt control over the untreted check. Tle 5. Study The fungicides in Tle 2 were pplied only once on August 18. Recovery in this study ws not s good s in Study I, which is proly due to the fct tht the plots only received one tretment nd, more importntly, due to the fct tht the tretments were mde over month fter the initil ppernce. of the disese, In study Tersn oz. drench; Clery's oz. drench nd 3 oz. folir nd Fungo 4 oz. drench gve significnt control compred to the untreted check. Tle 6. 17
12 Tle 5. Control of Anthrcnose in ~ nnu Percent infected re Rte/00 1 Tretment ft 2 I I Averge DPX oz Tersn 1991 (0 2 ) 4 oz Scotts Fertilizer + DSB 1 x Tersn oz Tersn 1991 (D) 8 oz DPX oz c Tersn oz c d Scotts F+F I x c d e Tersn LSR 8 oz c d e Scotts tv 1 x c d e Am. Nitrte c d e Dconi I oz c d e Tersn 75 4 ozo c d e f Dconi oz d e f Tersn LSR 4 oz d e f Dyrene 4 oz e f Check e f Dyrene 8 oz', e f Tersn LSR 6 oz e f Cl-Clor 2 oz e f Cl-Clor 1 oz f I Tretments followed y the sme letter re not significntly different from ech other t the 5% level. 2Symol (D) signifies tretment ws Drenched in. 18
13 Tle 6. Cont rol of Anthrcnose in Po nnu rte 1 Tretments 00 sq. ft. I I Averge Tersn 1991 (D)z 2 oz Clery's 3336 (D) 2 oz Clery's oz Fungo 50 (D) 4 oz Clery' s 3336 (D) 4 oz c Clery's 3336 (D) oz c d Fungo 50 (D) 1 oz c d Fungo 50 (D) 2 oz ,25.0 c d Clery's 3336 oz c d Tersn 1991 (D) oz c d Fungo 50 oz c d R.P oz c d Check c d Fungo 50 3 oz d 1 ' Tretment followed y the sme letters re not significntly different from ech other t the SO,!o level. 2(0) signifies tretment ws drenched in. 19
14 6. Fusrium Blight Study The 1975 Fusrium light studies were conducted t the Pele Creek Aprtments in Frmington, Michign nd t the Michign Stte University Crop nd Soil Science field lortories on Merion Kentucky luegrss. The plots t Pele Creek were 5 x feet wheres those t M.S.U. were 5' x 6'. Both sets of plots were replicted in rndom lock design. The liquid ppliction ws mde with n Ortho hose jr ppli~tornd the dry grnulr pplictions were mde with 3 foot Scotts drop spreder. All tretments were inmeditely drenched in fter ppliction. Pele Creek The tretments t Pele Creek were pplied on July 2, 16 nd August 22. The redings were tken on Sept. 23. The results in Tle 7 show tht Clery l s 3224, Tersn oz. with nd without wetting gent, Aqu Grow 8 oz., R.P rnked t the top of the tretments. The study ws conducted on hevy cly soil nd the wetting gents pprently helped with the penetrtion of the fungicide nd/or wter into the soil. M.S.U. Soils Field Lortory The tretments were pplied on August 28 nd Septemer 11 nd the redings were tken on Oct. 15. The results in Tle 8 shows Tersn oz, Tersn oz plus Hydro-wet; Tersn oz plus Aqu-Gro gve significnt control of Fusrium light compred to the untreted check. 20
15 Tle 7. PEBBLE CREEK Fusrium light study. % re infected Chemi cl Rte I I T AV -,.,.,'*'\.. :;;? Clery's Tersn 1991 & Aqu-Gro Tersn 1991 & Hydro..Wet Aqu-Gro Tersn 1991 & Hydro-Wet 4 oz R.P Tersn Tersn 1991 & Aqu-Gro Tersn Fo rm-a-tu rf R.P Form-A-Turf EL EL Hydro-Wet Check
16 Tretment TABLE 8 FUSARIUM BLIGHT STUDY SOILS FARM Rte/ 2 Percent infected re 00 ft I Averoe Tersn oz Scotts F x Tersn 1991 & Hydro':'Wet 8 oz c Tersn 1991 & Aqu-Gro 8 oz c Vydte c d Dsnit c d e Tersn 1991 & Aqu-Gro 4 oz c d e f Tersn 1991 & Hydro-Wet 4 oz c d e f Nemcur c d e f 2 Mocp c d e f Clery's z c d e f Hydro Wet 8 oz c d e f R.P oz c d e f R.P oz c d e f 9 Tersn oz c d e f 9 Aqu-Gro 8 oz c d e f 9 Ci-Geigy GA c d e f 9 Form-A-Tu rf 4 oz c d e f 9 CHECK d e f 9 Form-A-Turf 8 oz d e f g EL oz e f 9 Ci-Geigy GA s f 9 EL oz Tretments followed y the sme letter re not significntly different from ech other t the 5% level 2. Phytotoxicity I 22
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