SUPPLEMENTARY INFORMATION

Transcription

1 788 SUPPLEMENTARY INFORMATION Tble S1. Description of Sclerotini sclerotiorum isoltes used in this study. N = Number of Isoltes. Key bbrevitions: wmn = white mold screening nursery, producer = producer field, unk = unknown cultivr. Country Stte Field Code Yer Host N USA CA wmn, Beryl, Bunsi, G USA CO producer, Pinto, Yellow 41 wmn GH 1 USA ID producer GH 1 USA MI wmn,,,, 11A, 37, 38, B074, Beryl, Bunsi, Cornell, G122, Orion, PO7863, WM31 producer,, BL, Blck, Fuji, GH, Merlot, 19 SR06233, unk, Vist, Zorro USA MN wmn, Beryl, Bunsi, G USA ND producer, unk 53 wmn Beryl, Bunsi, G122 7 USA NE wmn,,, Beryl, Bunsi, G122, PO7683, 27 unk producer,,, Beryl, Emerson, GH, Orion, 20 Pinto, Weihing USA NY producer GH 1 USA OR wmn, Beryl, Bunsi, G producer G122, GH 2 USA WA wmn,,, 11A, 37, 38, Beryl, Bunsi, Cornell, G122, Orion, PO7 4, PO7863, WM31 producer, GH, Merlot, Pinto, Redkid 23 USA WI producer GH 2 Mexico - wmn Beryl, Bunsi, G Frnce - wmn, Beryl, Bunsi, G producer 2012 unk 4 Austrli - wmn Beryl, Bunsi, G122 4 producer Beryl Tble S2. Men ggressiveness rtings for Regions with more thn five smples; groupings ccording to 95% fmily-wise confidence intervl. Region Men Aggressiveness Group MN 5.84 ND 5.77 NE 5.29 b MI 5.13 bc OR 4.84 bcd CO 4.72 bcd WA 4.67 cd Frnce 4.66 cd Mexico 4.58 cd Austrli 4.12 cd CA 4.01 d 22/27

2 Tble S3. Men ggressiveness rtings for the most bundnt MCG; groupings ccording to 95% fmily-wise confidence intervl. MCG Men Aggressiveness Group b b b b b b b b b Tble S4. Men ggressiveness rtings for the MLH most bundnt; groupings ccording to 95% fmily-wise confidence intervl. MLH Men Aggressiveness Group b b b b b b b b 23/27

3 Figure S1. Exmple of MCG test pltes showing (A) comptible rection with myceli from two strins overgrowing ech other nd (B) n incomptible rection with brrge line of ded tissue forming between the two strins. Photo Credit: Rebecc Higgins. NE NY MN MI OR WA CO WI Frnce ND Aggressiveness Multilocus Hplotype MLH 140 MLH 163 MLH 25 MLH 65 MLH 66 Other MCG Figure S2. Strip plot of ggressiveness for the eight most bundnt MCGs prtitioned by region. Filled circles indicte one of the five most bundnt MLHs nd open circles indicte MLH of lesser bundnce. 24/27

4 community Community A Community B Community C Other Communities (n < ) weight 5 15 type Multilocus Genotype Mycelil Comptibility Group Figure S3. Grph showing complex ssocitions between Mycelil Comptibility Groups (MCG) (dotted nodes) nd Multilocus Hplotypes (MLH) (full nodes) where the number in ech node represents the MLH/MCG ssignment. Node size reflect the number of smples represented by ech node (circle). Edges (rrows) point from MLH to MCG where the weight (thickness) of the edge represents the number of smples shred. Node color represents the community ssignment bsed on the wlktrp lgorithm with mximum of four steps (Pons & Ltpy, 2006). An interctive version of this network cn be recreted using the code in the Interctive visuliztions section of the mlg-mcg.md file in the supplementry informtion (Direct Link: mlg-mcg.md#interctive-visuliztions) (Kmvr et l., 2017). 25/27

5 CA (12 15) WA (28 57) Frnce ( 16) Austrli (4 6) Number of MLHs OR (7 16) MI (42 55) MN (6 8) Popultions per MLH NE (22 34) Probbility of second encounter 1.6e 04 CO (19 36) 3.3e e 06 ND (27 47) 2.1e e 12 Number of MLHs in Region Number of privte MLHs Figure S4. Network of popultions (nodes/circles) nd their shred multilocus hplotypes (MLH) (edges/lines) hplotyped over 16 loci. Ech node is lbeled with nme (number of MLHs shred/number of MLHs totl). The shde nd re of the nodes re proportionl to the number of unique MLHs within the node nd the inner nodes re proportionl to the number of privte MLHs to the region (bottom legend). Ech edge represents single MLH where its thickness represents the number of popultions tht shre the MLH nd the shde represents the vlue of P sex, or the probbility of encountering tht MLH from two independent meiotic events. 26/27

6 NE NY MN MI OR WA CO WI ID Austrli CA Frnce Mexico ND Yer 2012 Figure S5. Sctter plot of Discriminnt Anlysis of Principl Components on Regions nd Yers showing temporl vrition cross ll Regions. Points (text lbels) represent observed individuls connected to the popultion centroids with ellipses representing 66% confidence intervl for norml distribution. The center of ech component is represented s blck grid lines. 27/27