Gilson, Inc. Table of Contents. Solid Phase Extraction Systems HPLC: Systems 2 9. Liquid Handlers HPLC: Liquid Handlers/Injectors 10 14

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2 Gilson, Inc. Table of Contents Designed for purification and engineered for science, Gilson, Inc. provides high quality, dependable solutions for today s demanding liquid chromatography, solid phase extraction, liquid handling and gel permeation chromatography clean-up requirements. From the legendary Gilson Pipetman products to a full range of system solutions, Gilson is the element of purification for your laboratory. Gilson s innovative products will revolutionize the lab; providing the durability, flexibility, modularity and ease of use the industry has come to expect from Gilson. Gilson has worldwide sales and service supporting the pharmaceutical, biotech, environmental, clinical, food and forensic markets. Our primary focus is on purifying samples via preparative HPLC, automated SPE or through GPC clean-up. Gilson focuses on early drug discovery and development with our preparative HPLC systems. Gilson automated SPE systems and GPC clean-up systems support environmental, food, clinical and forensic markets performing a wide variety of clean-up applications. Gilson offers complete solutions including the revolutionary control software, Trilution. Trilution LC Software is a complete HPLC software package designed specifically to meet purification needs. Trilution LH Software is designed to make automation of liquid handling and SPE applications simple and dependable. Gilson, Inc. world headquarters is located in Middleton, WI and is responsible for research, development and manufacturing of all Gilson instrumentation and software in addition to providing direct sales to the U.S. Gilson S.A.S., located outside of Paris, France, is responsible for all development and manufacturing of Gilson pipetting products and benchtop laboratory products. Gilson solutions are sold and supported worldwide. HPLC: Systems 2 9 GX-281 Preparative HPLC System... 5 GX-281 Analytical to Semi-preparative HPLC System... 6 GX-271 Preparative HPLC System... 7 GX-271 Analytical to Semi-preparative HPLC System Zprep Semi-preparative to Preparative HPLC System... 9 clc Analytical to Preparative HPLC System... 9 HPLC: Liquid Handlers/Injectors GX-281 Liquid Handler/Injector...11 GX-271 Preparative Liquid Handler/Injector...12 GX-271 Analytical Liquid Handler/Injector...13 GX-271 Liquid Handler/Injector without pump Liquid Handler/Injector...14 Multiple Probe 215 Liquid Handler/Injector...14 HPLC: Pumps /334 Prep-scale HPLC Pumps /332 Semi-preparative HPLC Pumps /322 Analytical to Semi-preparative HPLC Pumps X Series HPLC Pumps Syringe Pumps...18 Minipuls Evolution Peristaltic Pump...19 Minipuls 3 Peristaltic Pump...19 HPLC: Detectors /172 Diode Array Detectors X Series UV/VIS Detectors UV Detector...22 prepels II Detector...22 HPLC: Fraction Collectors prepfc Fraction Collector...24 FC 203B/FC 204 Fraction Collectors...25 Control Software Trilution LC Software...27 Trilution LH Software...28 GPC Clean-up Systems Automated GX-271 GPC Clean-up System...30 GX-271 GPC/SPE Clean-up System...31 Manual GPC Clean-up System...32 Solid Phase Extraction Systems GX-271 ASPEC, with 406 Single Syringe Pump...34 GX-271 ASPEC, with 406 Dual Syringe Pump...34 GX-274 ASPEC, with two 406 Dual Syringe Pumps...35 Quad-Z 215 Liquid Handler for SPE...36 SPE 215 System...37 Code 300 Series Racks...37 Liquid Handlers GX-281 Liquid Handler...39 GX-271 Preparative Liquid Handler...40 GX-271 Analytical Liquid Handler...41 GX-271 Liquid Handler without pump...41 GX-274 Liquid Handler...42 Quad-Z 215 Liquid Handler...43 Quad-Z 215 Liquid Handler with Disposable Tips...43 Multiple Probe 215 Liquid Handler Liquid Handler Sample Changer...45 Accessories VALVEMATE II Valve Actuator...47 Orbital Shaker...48 RackCount System AutoMix /853/854 Peltier Racks /542 Peltier Racks...50 Racks - Standard and Custom...50 References Gilson Rack/Instrument Compatibility Chart Gilson Probe Selection Guide Periodic Table...59 Flow Cell Selection Chart...59 ph of Common Acids and Bases...60 Column Sizes and Capacities...60 Maximum UV Absorbance for Commonly Used Solvents...61 Optimizing Preparative HPLC...62 Separation Scale-up Equations...62 Solvent Miscibility Table...63 Solvent Polarity Chart...63 Common Conversion Tables Gilson Applications

3 Gilson HPLC Systems Gilson high performance liquid chromatography (HPLC) systems provide complete solutions for all purification needs. Front-end liquid handlers and autosamplers are available in a range of bed capacities and optimized bench space footprints. Several models also accommodate innovative injection and fraction collection capabilities within a single platform. Trilution LC Software completely controls the entire HPLC system, allowing focus to remain on the science rather than the software. Gilson s VALVEMATE II Valve Actuator offers multiple column selection availability featuring: fast switching times, high pressure valve control of 6 or 10-port, 2, 6 or 10-position and low pressure valve control of 6 or 8-positions. (see page 47) The newest innovation from Gilson, the GX-271 Liquid Handler, is a mid-size capacity and throughput instrument fulfilling numerous laboratory needs at an economical price. (see pages 7, 8, 12 & 13) The 215 Series Liquid Handlers, known as the workhorses of the industry, are available in single, multiple and variable spanning probe models. (see page 14) Gilson s accurate pumping solutions are available with multi-solvent selection, high pressure variable mixing and interchangeable pump heads. (see pages 15 19) The GX-281 Liquid Handler is Gilson s premier front-end instrument featuring a large bed capacity and a wide array of components such as automatic reinjection of fractions using innovative GX Z-Injection technology. (see pages 5, 6, & 11) Gilson s variety of detection solutions offers excellent sensitivity and reproducibility including diode array (DAD), ultraviolet (UV) and/or visible (VIS) and evaporative light scattering (ELS) detection. (see pages 20 22) Visit or call for more details on Gilson HPLC Systems.

4 HPLC: Systems Column ID 2 mm 4.6 mm 10 mm 20 mm 30 mm 50 mm 100 mm Description Page Number The GX-281 Preparative HPLC System allows for injection, fraction collection and reinjection. The system is also ideal for liquid handling techniques such as sample mixing, dilutions and sample extract transfers. Sample Loading Capacity 15 g/injection Injection Loop Volume 250 µl 25 ml Optimal Column Size mm ml/min Maximum Injection Pressure 3,000 psi (207 bar) Maximum Pump Pressure 3,040 psi (210 bar) GX-281 Preparative HPLC System GX-281 Analytical to Semi-preparative HPLC System GX-271 Preparative HPLC System GX-271 Analytical to Semi-preparative HPLC System flow rate ml/min injection loop volume 250 µl 25 ml sample mass 15 g/injection flow rate ml/min injection loop volume 2 µl 2 ml sample mass 300 mg/injection flow rate ml/min injection loop volume 250 µl 25 ml sample mass 15 g/injection flow rate ml/min injection loop volume 2 µl 5 ml sample mass 300 mg/injection The GX Z-Injection Module allows sample aspiration directly into the valve without a separate injection port, eliminating a source of potential carryover. GX Direct Injection technology features an injection port that connects directly to the valve, minimizing volume associated with connective tubing. (located behind the racks) The prep-scale HPLC pumps feature low-dwell volumes which allow for fast, reproducible gradients. (see pages 15-19) 845Zprep Semi-preparative to Preparative HPLC System clc Analytical to Preparative HPLC System flow rate ml/min injection loop volume 100 µl 10 ml sample mass 15 g/injection flow rate ml/min injection loop volume 5 µl 25 ml sample mass 15 g/injection flash chromatography 9 9 The GX Prep Solvent System features a dynamic range of volumes and flow rates using syringeless technology within a solvent delivery pump capable of accessing five off-bed solvents. Visit or call for more details on Gilson HPLC Systems. 5

5 HPLC: Systems The GX-281 is a multi-functional instrument that can perform injection, fraction collection and reinjection. Sample Loading Capacity 300 mg/injection Injection Loop Volume 2 µl 2 ml A single, mid-size instrument for injection and fraction collection with separate fluid pathways to reduce cross-contamination of fractions. Sample Loading Capacity 15 g/injection Injection Loop Volume 250 µl 25 ml The GX-281 features a Jet Wash Rinse station design that delivers individual streams of solvent for each separate rinse station to actively wash the outside of probes. Four rinse solvents are available for selection. The GX Z-Injection Module allows fast injections with low carryover and low maintenance by aspirating the sample directly into the valve. Optimal Column Size 2 20 mm Maximum Injection Pressure 5,000 psi (345 bar) Separate injection and fraction collection paths reduce cross-contamination of fractions from sample injection. The cycle time of the injection is minimized by eliminating rinse steps which are required with single fluid path configurations. H1: 150 µl/min 15 ml/min H2: 30 µl/min 30 ml/min Maximum Pump Pressure H1: 8,700 psi (600 bar) H2: 4,350 psi (300 bar) Semi-preparative HPLC pumps are offered to perform binary gradients. (see pages 15 19) The GX Prep Solvent System uses syringeless technology and allows access to five off-bed solvents. Optimal Column Size mm Maximum Injection Pressure 3,000 psi (207 bar) The GX Direct Injection Module can utilize both 1/16 and 1/8 injection valves, allowing for a wide variety of sample volumes and loop options. A VALVEMATE II Valve Actuator can be incorporated for multiple column selection or column regeneration applications. (see page 47) ml/min Maximum Pump Pressure 3,040 psi (210 bar) Binary gradient preparative HPLC pumps provide high-flow capabilities. (see pages 15 19) Detection solutions are available for DAD, fixed or variable wavelength UV/VIS, UV and ELS detection. (see pages 20 22) Mid-to-large collection capacity in a small footprint. Modular detector options available. (see pages 20 22) Visit or call for more details on Gilson HPLC Systems. 7

6 HPLC: Systems The GX-271 is a mid-size liquid handler/fraction collector with a bench saving footprint featuring separate fluid pathways that reduce cross-contamination of samples and fractions. Sample Loading Capacity 300 mg/injection Optimal Column Size 2 20 mm Maximum Injection Pressure 5,000 psi (345 bar) Injection Loop Volume 2 µl 5 ml H1: 150 µl/min 15 ml/min H2: 30µL/min 30 ml/min Maximum Pump Pressure H1: 8,700 psi (600 bar) H2: 4,350 psi (300 bar) Gilson s 845Zprep System features Gilson s first generation Z-Injection technology accommodating volumes and flow rates for semi-preparative to preparative applications. Mid-to-large bed capacity, accommodating both samples and fractions. Sample Loading Capacity 15 g/injection Maximum Injection Pressure 3,000 psi (207 bar) Optimal Column Size mm Maximum Pump Pressure 3,040 psi (210 bar) The GX Analytical Solvent System features innovative syringeless technology and access to five off-bed solvents. Binary gradients are achieved with analytical to semi-preparative HPLC pumps. (see pages 15 19) For confirmation or added detection capabilities of nonchromaphoric compounds configure an additional ELS detector with a multi-channel DAD detector. (see pages 20 22) Injection Loop Volume 100 μl 10 ml Injection Variability < 1.5% Carryover < 0.01% ml/min Gilson s clc System is a complete chromatography system capable of automating the purification of compounds using both normal and reversed-phase columns. The clc System has the ability to accommodate various modes of detection including DAD, UV/VIS, ELS and RI. Additional analytical components are also integrated onto the clc System enabling automated on-line evaluation of the collected fractions for purity and recovery. The low mount collection valve enables fraction collection to tubes and contains two separate liquid paths which reduce cross-contamination. Sample Loading Capacity 15 g/injection Optimal Column Size mm Maximum Injection Pressure 5,000 psi (345 bar) Injection Loop Volume 5 µl 25 ml ml/min Maximum Pump Pressure 33X Pump: 3,040 psi 32X Pump: 8,700 psi Visit or call for more details on Gilson HPLC Systems.

7 HPLC: Liquid Handlers/Injectors GX-281 Liquid Handler/ Injector GX-271 Preparative Liquid Handler/Injector GX-271 Analytical Liquid Handler/Injector 215 Liquid Handler/Injector Multiple Probe 215 Liquid Handler/Injector Liquid Handler Fraction Collection Injection Volume Liquid Handling Injection Carryover Gilson s liquid handlers and injectors offer the broadest range of instrument capabilities on the market today. From small footprint injectors to larger footprint liquid handler/injector/fraction collectors, Gilson provides the ultimate front-end automation solutions for every HPLC system. Page Number 250 µl 25 ml 98% (100 µl 25 ml) < 0.005% µl 25 ml 98% (50 µl 25 ml) < 0.05% 12 2 µl 2 ml 98% (10 µl 25 ml) < 0.05% 13 5 µl 10 ml 99.7% (50 µl) < 0.1% 14 5 µl 5 ml 99.2% (500 µl) < 0.05% 14 Specifically engineered for semi-preparative to preparative HPLC requirements, the GX-281 offers unmatched versatility and performance for front-end LC automation including revolutionary syringeless pumping technology. GX-281 Liquid Handler/Injector Bed Capacity Tube Diameter Immediate response aspiration and dispensing with the GX Prep Solvent System eliminates time spent waiting for valves to switch and syringes to fill. Sample Quantity 13 mm mm mm mm mm mm 162 Note: tubes up to 220 mm tall can be accommodated (see page 51 for the Gilson Rack/Instrument Compatibility Chart) % Mobile Phase mvolts Two unique and separate liquid pathways for injection and fraction collection provide a zerocontamination environment. < 1% CV (total loop) < 3.5% (partial loop) 98% (100 µl 25 ml) Injection Loop Volume 250 µl 25 ml Injection Carryover < 0.005% Injection, collection and analysis Fraction Zone, 1 Fraction Zone, 2 Fraction Zone, 3 Fraction Zone, 4 Fraction Zone, 5 Fraction Zone, 6 Fraction Zone, 7 Fraction Zone, 8 Fraction Zone, 9 Fraction Zone, 10 Time (minutes) The GX Direct Injection Module features an injection port that connects directly to the injection valve for decreased dead volume and reduced maintenance ml/min Maximum Injection Pressure 5,000 psi (345 bar) 87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in) Fraction Zone, 11 Fraction Zone, 12 Product #s: (GX-281 Liquid Handler) (GX-281 Liquid Handler with bar code) (GX Direct Injection Module, 1/8", prep) (GX Direct Injection Module, 1/16", prep) (GX Direct Injection Module,1/16", analytical SS) (GX Direct Injection Module, 1/16", analytical PEEK) (GX Z-Injection Module) Visit or call for more details on Gilson HPLC Liquid Handlers/Injectors. 11

8 HPLC: Liquid Handlers/Injectors 1 µl/min 50 ml/min 98% (50 µl 25 ml) GX-271 Preparative Liquid Handler/Injector Injection Loop Volume 250 µl 25 ml Injection Carryover < 0.05% < 1% CV (total loop) Maximum Injection Pressure 5,000 psi (345 bar) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) The GX-271 Liquid Handler/Injector is the perfect blend of size, performance and capacity, consuming only 23" of linear bench space. Large-bore injection modules feature a continuous flow path design for preparative applications. Small-bore valves are available for analytical to semi-preparative applications with inert injection modules for biocompatible applications. The GX-271 Liquid Handler/Injector can be configured for large injection loop volumes with minimal sample waste and carryover as well as to accommodate either the GX Prep or Analytical Solvent System which feature syringeless pumping technology. 1 µl/min 5 ml/min 98% (10 µl 25 ml) GX-271 Analytical Liquid Handler/Injector Injection Loop Volume 2 µl 2 ml Injection Carryover < 0.05% < 1% CV (total loop) < 3.5% (partial loop) Maximum Injection Pressure 5,000 psi (345 bar) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) % Mobile Phase mvolts Recovery data: Theophylline (5 mg/ml), Caffeine (5 mg/ml) and Phenol (3 mg/ml) solution with 1 ml injection loop volumes calculated from reinjection of collected Theophyline fractions (Zone 2) Fraction Zone, 1 Fraction Zone, 2 Fraction Zone, Ethernet based communication provides better connectivity. Jet Wash Rinse technology provides flowing rinse for fast and efficient rinsing protocols utilizing up to two separate flowing rinse solvents. The GX Direct Injection Module increases performance by incorporating a port that connects directly to the injection valve, reducing sample waste and maintenance. % Mobile Phase mvolts Series of ten 20 µl total loop injections of Theophylline, Caffeine and Phenol Phenol Theophylline Caffeine Time (minutes) Time (minutes) Injection Recovery % % % % % % % % % % Average Recovery 99.99% Standard Deviation 0.01 CV 1.39% Available in an analytical or prep model, the solvent systems feature dynamic flow rate ranges and can access five off-bed solvents. Peak Retention Time (minutes) Area Count (µvmin/100) Standard Deviation Theophylline % Caffeine % Phenol % Separate fraction collection flow paths prevent contamination from the injection process. Product #s: (GX-271 Preparative Liquid Handler) (GX-271 Analytical Liquid Handler) (GX-271 Liquid Handler without pump) (GX Direct Injection Module, 1/8, prep) (GX Direct Injection Module, 1/16, prep) (GX Direct Injection Module,1/16, analytical SS) (GX Direct Injection Module, 1/16, analytical PEEK) CV GX-271 Preparative and Analytical Liquid Handler/Injector Bed Capacity Tube Diameter Sample Quantity 10 mm mm mm mm mm mm mm 70 Note: tubes up to 200 mm tall can be accommodated (see page 51 for the Gilson Rack/Instrument Compatibility Chart) Visit or call for more details on Gilson HPLC Liquid Handlers/Injectors. 13

9 HPLC: Liquid Handlers/Injectors HPLC: Pumps This versatile, large bed liquid handler accommodates more than 3,000 racks to hold virtually any sample vessel. Customize by adding accessories and components to meet laboratory needs. The 215 Liquid Handler/Injector, a true and trusted laboratory workhorse for many years, performs injection, fraction collection and reinjection of collected fractions within a single platform. Pressure 1 mm Column ID 2 mm 4 mm 10 mm 20 mm > 50 mm Page Number Product #s: (215 Liquid Handler) (819 Injection Module) (845Z Injection Module) (845Zprep Injection Module) 333/334 Prep-scale HPLC Pumps ml/min 3,040 psi (210 bar) 16 liquid handling: 99.7% (50 μl) Injection Loop Volume 5 μl 10 ml liquid handling: 1.37% CV (50 μl) injection: < 0.9% (1 ml) (total loop) Injection Carryover < 0.1% 91 x 61 x 56 cm (36 x 24 x 22 in) 331/332 Semi-preparative HPLC Pumps 321/322 Analytical to Semi-preparative HPLC Pumps ml/min H1: ml/min H2: ml/min 8,700 psi (600 bar) H1: 8,700 psi (600 bar) H2: 4,300 psi (300 bar) X Series HPLC Pumps 10 µl/min 200 ml/min 8,700 psi (600 bar) 18 Inject onto FIA/MS, parallel HPLC and MUX systems using the optional 849 Multiple Injection Module. The Multiple Probe 215 Liquid Handler/Injector is capable of high-throughput automation of all basic liquid handling tasks such as sample transfer, dilutions, mixing, reagent addition and plate reformatting. 402 Syringe Pumps Minipuls Evolution Peristaltic Pump 1 µl/min 120 ml/min 0.2 µl/min 340 ml/min 110 psi (8 bar) for ml syringes 40 psi (3 bar) for 25 ml syringe 42 psi (3 bar) Product #: (Multiple Probe 215 Liquid Handler) Configure with the 0.015" ID analytical valve or the 0.040" ID preparative valve. Product #: (849 Multiple Injection Module) The 849 Multiple Injection Module allows four simultaneous injections, ideal for parallel HPLC and MUX systems. liquid handling: 0.57% CV (500 μl) injection: < 5% CV (500 μl) (partial loop) method: < 2% CV (500 μl) (total loop) liquid handling: 99.2% (500 μl) Injection Loop Volume 5 μl 5 ml Valve Switching Speed RV : < 135 ms RV : < 370 ms Injection Carryover < 0.05% 97.8 x 61 x 55.8 cm (38.5 x 24 x 22 in) Multiple Injection Module Option 61 x 18 x 13 cm (24 x 7 x 5 in) Minipuls 3 Peristaltic Pump standard (1, 2, 4 or 8 channels): 0.3 µl/min 30 ml/min high-flow rate (2 or 4 channels): ml/min standard: 72 psi (5 bar) high-flow rate: 42 psi (3 bar) 19 Gilson offers unsurpassed flexibility in HPLC pumping systems with an extensive product range and accommodations. Visit or call for more details on Gilson HPLC Pumps. 15

10 HPLC: Pumps Capable of fast separations with high-efficiency columns in normal and reversed-phase modes, Gilson s 333 and 334 Pumps are the premier solvent delivery solutions for preparative chromatography. Choose the 333 master pump for isocratic applications or the 333/334 binary system for composition gradient with high pressure mixing (third pump for ternary). Stackable to conserve bench space ml/min (3,040 psi) < 0.5% or 2.7 μl/min RSD ± 1% or 5.3 μl/min 333: 26 x 41 x 51 cm (10 x 16 x 20 in) 334: 26 x 41 x 39 cm (10 x 16 x 15 in) Gilson s 331 and 332 dual-piston pumps for semi-preparative and preparative chromatography feature a variable volume, dynamic mixer for optimized gradient performance. Trilution LC Software provides control within a complete semi-preparative system including data processing and result reporting or stand-alone control via the keypad incorporated on the 331 Pump. Smooth linear transposition from analytical to preparative HPLC methods using 4 to 20 mm ID columns packed with highefficiency stationary phases. Milligram-level sample mass-throughput per injection; 150 mg with a 20 mm bore column. Solvent selection on each pump with optional 4-solvent valve for up to eight solvents. Programmed Composition (% E) mvolts Gradient Linearity and Time (minutes) Capable of milligram-to-gram level mass-throughput per injection up to 15 depending on column size and load capacity. Selection of up to four solvents per pump for a total of eight solvents in a binary pumping system. Retention Time Repeatability Time (minutes) Series of Ten Injections Detected Composition (mau) % Mobile Phase Methyl PB Ethyl PB Propyl PB Average Retention Time Standard Deviation CV 0.72% 0.34% 0.29% The entire preparative HPLC system is controlled with Trilution LC Software, additionally the 333 model has an incorporated keypad for stand-alone operation. The flow rate can be doubled with additional parallel pumps to 400 ml/min. Product #s: (left) (333 Pump, H3 primary solvent, dual-piston, reciprocating master pump) (right) (334 Pump, H3 secondary solvent, dual-piston, reciprocating remote controlled pump) Product #s: (right) (331 Pump, H2 primary solvent, dual-piston, reciprocating master pump) (left) (332 Pump, H2 secondary solvent, dual-piston, reciprocating remote controlled pump) The 321 and 322 multi-solvent pumps are designed for use with Gilson s HPLC analytical and semi-preparative chromatography systems. Product #s: (321 Pump, H1) (left) (322 Pump, H1) (right) (321 Pump, H2) (322 Pump, H2) H1 pump heads are ideal for 2 to 10 mm ID columns. H2 pump heads are ideal for 4 to 20 mm ID columns ml/min (8,700 psi) H2: ± 0.7% or 7 μl/min ± 2% or 13 μl/min 331: 26 x 41 x 51 cm (10 x 16 x 20 in) 332: 26 x 41 x 39 cm (10 x 16 x 15 in) High pressure Adjustable Volume Dynamic Mixer (AVDM ) enables fast gradient response times and homogeneous mixtures for analytical to semi-preparative applications. While the pumps are controlled via Trilution LC, the 321 also incorporates a keypad that allows independent stand-alone gradient control. A single 321 or 322 Pump is capable of binary gradients. H1: ml/min (8,700 psi) H2: ml/min (4,300 psi) H1: < 0.7% or 2 µl/min H2: 4 µl/min ± 2% or 13 μl/min 321: 26 x 41 x 51 cm (10 x 16 x 20 in) 322: 26 x 41 x 39 cm (10 x 16 x 15 in) Visit or call for more details on Gilson HPLC Pumps. 17

11 HPLC: Pumps Together with Gilson s manometric modules and dynamic mixers, the 305, 306 and 307 Pumps offer a cost-effective, space saving and robust solution for isocratic or gradients of analytical to preparative HPLC. The 307 model functions as an isocratic pump with a built-in manometric module. Dynamic mixers ensure efficient, dependable gradient creation. The 306 model functions as a slave unit, controlled by either a 305 or Trilution LC Software. The 305 and 307 Pumps are programmable through the incorporated keypad. Tools are not required for pump head removal, allowing accessibility for maintenance. Piston seals, check valves and other parts can be replaced in a matter of minutes. Offering unmatched flexibility and performance for liquid transfer applications, the Minipuls Evolution is designed for scientists seeking a high performance, manageable peristaltic pump capable of a variety of configurations. Gilson offers the only peristaltic pump that allows up to 16 separate channel cartridges to be changed while the pump is running with a simple push on the cartridge for placement and removal. 0.1% on all channels Tubing ID standard: mm high-flow: 2 8 mm Optimized six roller drive limits pulsation. standard: 0.2 µl/min 70 ml/min (42 psi, 3 bar) high-flow: ml/min (29 psi, 2 bar) 305/306: 10 μl/min 200 ml/min 307: 10 μl/min 10 ml/min < 1% 33 x 33 x 15 cm (13 x 13 x 6 in) < ± 1% Product #s: (305 Pump) (right) (306 Pump) (left) (307 Pump) Product #: F (Minipuls Evolution Pump) Head Speed continuous adjustment from 0 60 rpm by 0.01 rpm increments up to 9.99 rpm, 0.1 rpm increments above 10 rpm Module: 164 x 182 x 169 mm MF1 pump head: 164 x 56 x 169 mm MF4 pump head: 164 x 92 x 169 mm HF2 pump head: 164 x 92 x 169 mm The 402 Syringe Pumps assure accuracy in sample transfers, dilutions, reagent additions, mixing and more. They offer speed and reliability for repetitive liquid handling tasks. Available in three configurations: 402 Single, ideal for sampling injectors. 402 Dual with Tee, provides rapid dilutions in both small and large volumes. An ideal addition to Gilson liquid handlers and injectors including the GX and 215 Series instruments. 402 Dual, two-in-one accommodates simultaneous dispensing of multiple reagents and solvents. The Gilson Minipuls 3 multi-channel peristaltic pump is simple to integrate onto existing systems with control through an incorporated keypad or remotely with a separate device. Enhanced reliability and ease of operation are possible because the pump s speed stability is maintained independent of normal temperature variations from 0 to 40 C. This makes the pump ideal for biological perfusion applications. (Note: not intended for direct connection to human subjects.) The Minipuls 3 accommodates wide flow rate ranges using either the standard or high-flow pump head. Pump heads are available with one, two, four or eight channels. Multiple stainless steel rollers drive the pump to produce smoother, low pulse flows. Product #s: F (left) (402 Single Syringe Pump) F (right) (402 Dual with Tee Syringe Pump) F (402 Dual Syringe Pump) 0.8% (10 μl) 98.2% (10 μl) 1 μl/min 120 ml/min 17 x 20 x 24 cm (6.7 x 7.9 x 9.4 in) Product #: F (Minipuls 3 Pump, single channel) standard: 0.3 μl/min 30 ml/min (72 psi, 5 bar) high-flow: ml/min (42 psi, 3 bar) Tubing ID standard: mm high-flow: 2 8 mm Head Speed continuous adjustment from 0 48 rpm by 0.01 rpm increments up to 9.99 rpm, 0.1 rpm increments above 10 rpm 15 x 17.5 x 18 cm (5.9 x 6.9 x 7.1 in) Visit or call for more details on Gilson HPLC Pumps. 19

12 HPLC: Detectors 171/172 Diode Array Detectors 15X Series UV/VIS Detectors 112 UV Detector Diode Array (DAD) Ultraviolet (UV) Visible (VIS) Evaporative Light Scattering (ELS) Wavelength or nm nm 9 nm 254 or 280 nm Spectral Band Width Channels Page Number or 12 nm dual & single 21 single 22 Gilson s recently released 171 and 172 Diode Array Detectors deliver rugged and accurate detection for both preparative and analytical HPLC. Detection is done with a fiber optic light path that can be configured in a fixed or remote flow cell design. Product #s: (171 Diode Array Detector) (172 Diode Array Detector) With remote flow cell, the power of the detector is located directly at the outlet of the column, which yields virtually no peak broadening. An interchangeable flow cell design allows for both the 171 and 172 Detectors to accommodate analytical and semi-preparative to preparative applications. Pixels 171: : 512 Wavelength 171: nm 172: nm Wavelength < 0.5 nm Baseline Drift 5 x AU/h S/N Ratio 171: < 3 x AU 172: < 2 x AU Pixel Distance 171: ~2.2 nm/pixel 172: ~0.8 nm/pixel Wavelength Repeatability < 0.1 nm Wavelength Temperature Drift < nm/ C 26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in) prepels II Detector single 22 Gilson offers a complete line of HPLC detection solutions that are ideal for analytical, semi-preparative and preparative HPLC applications. Gilson s 151, 152, 155 and 156 UV/VIS Detectors deliver versatile, rugged detection with an interchangeable flow cell design to accommodate analytical to preparative HPLC applications. (see page 59 for Flow Cell Selection Chart) The following modes are available to specify detection conditions and basic operating parameters: Single wavelength with dual sensitivities (151 and 152) Dual wavelengths (155 and 156) Scan wavelengths (155 and 156) Choose either Gilson s Trilution LC Software or the standalone keypad control on the 151 and 155 Detectors. Up to ten methods can be stored mvolts Time (minutes) % Moblie Phase Product #s: (right) (151 UV/VIS Detector, with flow cell/accessory kit) (152 UV/VIS Detector, with flow cell/accessory kit) (155 UV/VIS Detector, with flow cell/accessory kit) (left) (156 UV/VIS Detector, with flow cell/accessory kit) Lamp UV: deuterium, 750 hrs VIS: tungsten/halogen, 500 hrs Wavelength nm Drift 3 x 10 4 AU/hr Sensitivity AU Spectral Band Width 9 nm Setting ± 2 nm Setting Precision ± 0.2 nm 26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in) Visit or call for more details on Gilson HPLC Detectors. 21

13 HPLC: Detectors The 112 UV Detector is a rugged, economical detector for analytical or preparative HPLC and GPC clean-up. A front panel dial allows selection of sensitivity level to adjust the absorbency of a sample. Accessible lamp/filter assemblies for selecting wavelengths. Fixed wavelength design for increased sensitivity and durability. 10 mm pathlength analytical flow cell provides excellent sensitivity ranging from to 1 AUFS (Absorbance Units, Full Scale). Reduced operating and setup time with a single step baseline function. Event-marking function conveniently marks injection point or fraction collector advance on either the recorder or data system. Large Capacity HPLC: Fraction Collectors Small Microplate Tubes 1 L Bottles Page Number prepfc Fraction Collector 200 ml/min 24 Product #: (112 UV Detector, with lamp and 11 μl flow cell/accessory kit) Lamp mercury (standard): 254 nm, 2,000 hrs phosphor-coated mercury: 280 nm, 500 hrs Spectral Band Width 254 nm: nm: 12 Drift 3 x 10 4 AU/hr Wavelength 254 or 280 nm Flow Cell Sensitivity < 4 x 10 4 AU for flow step of ml/min (Methanol) 32 x 32 x 13 cm (12.6 x 12.6 x 5.2 in) FC 204 Fraction Collector FC 203B Fraction Collector 20 or 200 ml/min 20 ml/min Gilson s evaporative light scattering (ELS) detector, prepels II, provides capabilities on the analytical-scale with the ability to detect as low as nanograms of analyte while a unique filtering technology removes baseline noise without distressing peak height or shape. mvolts ELSD UV Time (minutes) % Moblie Phase The prepels II Detector is ideal for obtaining the purest fractions with the ability to detect virtually any analytes with or without a chromophore, including compounds that have poor UV absorption such as sugars, carbohydrates, lipids and triglycerides. Gilson offers advanced solutions for fraction collection with a complete line of stand-alone and software-controlled fraction collectors ranging from small footprint to large vessel capacity models and everything in between. Light Source 670 nm laser diode, < 5 mw Gas Requirement 65 ± 5 psi Nitrogen or Argon Nebulization Temperature 0 70 C Evaporative Zone Temperature ambient 120 C Liquid ml/min Splitting packages available to accommodate system flow rates up to 75 ml/min Dimensions 25 x 46 x 29 cm (9.8 x 18 x 11.5 in) Product #s: (prepels II Detector, 120V) (prepels II Detector, 240V) Visit or call for more details on Gilson HPLC Fraction Collectors. 23

14 HPLC: Fraction Collectors Gilson s prepfc Fraction Collector is a large capacity, compact footprint fraction collector ideal for semi-preparative to preparative HPLC applications. The prepfc features an adjustable tray that accommodates various vessel heights, volumes and sizes. Sample Quantity (20 ml) scintillation vials (4) Code 333 Racks x 75 mm (4 ml) tubes (4) Code 341 Racks 150v mvolts x 100mm (9 ml) tubes (4) Code 343 Racks Minutes prepfc Bed Capacity Size Vessel Rack x 100 mm (9 ml) tubes (4) Code 343 Racks x 100 mm (12 ml) tubes (2) Code 223 Racks x 150 mm (25 ml) tubes (4) Code 22 Racks x 200 mm (70 ml) tubes (2) Code 239 Racks x 57 mm (25 ml) scintillation vials (2) Code 204 Racks x 115 mm (50 ml) conical bottom tubes (2) Code 222 Racks x 113 mm (125 ml) bottles (2) Code 211 Racks 11 (500 ml) bottles (1) Code 91 Rack 81 (1 L) bottle (1) Code 92 Rack x 32 mm (2 ml) vials (4) Code 330 Racks x 150 mm (20 ml) tubes (4) Code 345 Racks (see page 51 for the Gilson Rack/Instrument Compatibility Chart) 50.3 x 39.1 x 34.8 cm (19.8 x 15.4 x 13.7 in) Liquid Contact Material PTFE and 316 stainless steel Valve Switching Speed < 20 ms prepfc Control Software: Remote start and tube advance Up to ten programmable collection windows Active chromatogram display with start and stop fraction collection lines Customizable collection patterns and rack addition Increased X/Y speed optimizes fraction collection. Valve Dead Volume 114 μl internal volume; 6 μl dead volume from common port to normally closed port 200 ml/min Collection Modes slope, level, time or volume High-flow fraction collection valve. Choose the compact FC 203B Fraction Collector for small capacity collection or the FC 204 for larger volumes. Collect fractions by time, drop or peak, accommodating all LC applications. Both models accept a wide variety of collection vessels including tubes, vials and microplates. The 3-way diverter valve prevents contamination of collected fractions (standard on FC 204 and optional on FC 203B). Up to ten programmable time windows can be added for collecting only samples of interest, while discarding the column s void volume, areas of non-interest and equilibration volumes. FC 203B: 32.4 x 29.2 x 26.7 cm (12.8 x 11.5 x 10.5 in) FC 204: 47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in) Liquid Contact Material PTFE and 316 stainless steel In peak mode, collectors monitor a detector signal and identify peaks using either an adaptive-slope algorithm or a specified mv threshold. The fraction collector utilizes multi-column adapters for collecting up to 18 separate channels. Its compact size fits in most fume hoods. Valve Switching Speed 100 ms FC 203B: 20 ml/min FC 204: 20 or 200 ml/min Valve Dead Volume FC 203B/FC 204: 64 μl internal volume; 3.5 μl dead volume from common port to normally closed port FC 204: 114 μl internal volume; 6 μl dead volume from common port to normally closed port Contact closure inputs are available to remotely start, advance or stop fraction collection. Pocket PC control for stand-alone operation allows the prepfc to be added to any HPLC system capable of sending contact closures. Increase bed capacity by configuring up to four fraction collectors in a single system (provides over 1,000 samples). Product #s: (prepfc Fraction Collector - Ethernet) (prepfc Fraction Collector) Product #s: (right) (FC 203B) DV (FC 203B, 3-way valve) (left) (FC 204) (FC 204, prep valve) Stand-alone instruments are controlled with an incorporated keypad or through Gilson s Trilution LC Software. Visit or call for more details on Gilson HPLC Fraction Collectors. 25

15 Control Software Gilson has redefined purification with the HPLC system control software, Trilution LC. This liquid chromatography software, specifically designed for purification, allows for complete control to achieve the challenging results that applications require. HPLC Systems SPE Systems GPC Clean-up Systems Liquid Handlers Page Number Trilution LC 27 Trilution LH 28 Configuration and Virtual Lab Screen Gilson offers powerful software to complement robust instruments. With accommodating features, drag-and-drop functionality and numerous supplied tasks, Gilson s software packages allow effortless yet complete control of instruments focusing on the science rather than the software. Intel, Pentium and Core are trademarks of Intel Corporation or its subsidiaries in the United States and other countries. Microsoft and Windows are registered trademarks of the Microsoft Corporation in the United States and/or other countries. Application Run Screen Fraction Collection Capabilities: Collection modes: slope, level, time or volume Conditional logic fraction collection Graphical fraction collection optimization Slope collection settings: front, tail and APEX Auto sample list generation Open Access Module: Administrator control and permissionsetting capabilities Walk-up user access Minimal training required Conforms to the application needs rather than compromising the application in order to fit the software. Method Builder Control Screen Computer Recommendation Processor Intel Pentium 4 (2.8 GHz); Intel Core 2 Duo (1.86 GHz) RAM 2 GB Fraction Collection Settings Page Product #s: R20 (Trilution LC Media) (Trilution LC Lifetime License) L5 (Trilution LC Multi-user Lifetime License - 5) L10 (Trilution LC Multi-user Lifetime License - 10) (Trilution LC 1 year License) Note: Requires Oracle Database 10g Express Edition Fixed Drive 120 GB, available space: 4 GB (6 GB at install) Additional Drive DVD/CD-RW Video Card 1280 x 1024 with 24 bit True Color Serial Port 1 Operating System Microsoft Windows XP Professional SP2 or later Microsoft Windows 2000 SP4 Visit or call for more details on Gilson Control Software. 27

16 Control Software Trilution LH Software, designed by scientists for scientists, is Gilson s complete control solution for liquid handling and solid phase extraction applications. Trilution LH features a user-friendly graphical interface with icon-based drag-and-drop functionality minimizing the learning curve and optimizing application performance. Fraction Collection GPC Clean-up Systems Multiple Fractions Solid Phase Extraction Control Page Number Liquid Disposable Solid Phase Solvent Tweaks Auxiliary Handling Tips Extraction System Product #s: R20 (Trilution LH Media) (Trilution LH Lifetime License) (Trilution LH 1 Year License) Note: Requires Oracle Database 10g Express Edition Automated GX-271 GPC Clean-up System ml (optional) Trilution LC Software 30 GX-271 GPC/SPE Clean-up System ml (optional) Trilution Software 31 Main Menu Manual GPC Clean-up System 0.25 < 1000 ml (optional) 307 Pump keypad 32 Method in Method Builder Screen Over 70 Gilson-supplied tasks enable quick and efficient method programming. Quick and effortless instrument setup, bed layout and method creation. Sample list with spreadsheet functionality and graphical run simulation. SS Dispense Task Property Page Run methods in either batch or sequential mode to optimize performance. Manual control for performing routine tasks such as priming instruments and troubleshooting. Graphical custom task creation and cartridge monitoring. Simulation Mode in Application Run Screen Computer Recommendation Processor Intel Pentium 4 (2.8 GHz) RAM 1 GB Fixed Drive 80 GB, available space: 4 GB (6 GB at install) Additional Drive DVD/CD-RW Video Card 1280 x 1024 with 24 bit True Color Serial Ports 2 Operating System Microsoft Windows XP Professional SP2 or later Microsoft Windows 2000 SP4 Gilson s gel permeation chromatography (GPC) clean-up systems are an ideal solution for postextraction clean-up of interfering substances from environmental samples, food products and animal tissue, prior to analysis by GC, GC/MS, HPLC or LC/MS. Failure to remove these interfering compounds can cause poor analytical results, shorten column life and increase analytical downtime. Gilson GPC Clean-up Systems meet clean-up guidelines set forth by the USEPA, USFDA, USDA, USGS, ISO, CFIA, AOAC International and European Union (EN 1528, EN 12393, S19) requirements. Visit or call for more details on Gilson GPC Clean-up Systems. 29

17 GPC Clean-up Systems The Automated GX-271 GPC Clean-up System from Gilson is capable of up to 80 sample extractions with large fraction collection capacity into an assortment of vessels. Gilson s newly released Automated GX-271 GPC/SPE Clean-up System provides SPE capabilities in addition to GPC for sample extract purification; a multi-purpose sample preparation system. Automated GPC Systems Bed Capacity Sample Quantity Description GPC Collection Rack Product # x 200 mm (170 ml) collection tubes Code 320 Rack x 200 mm (70 ml) collection tubes Code 321 Rack x 137 mm (250 ml) Boston Round bottles Code 322 Rack Labconco RapidVap (450 ml) flat-bottom evaporation flasks Code 323 Rack Caliper TurboVap II (200 ml) evaporation tubes Code 324 Rack Labconco RapidVap (450 ml) evaporation flasks, with stem Code 325 Rack Caliper TurboVap II (50 ml) evaporation tubes Code 326 Rack Labconco RapidVap (100 ml) flat-bottom evaporation flasks Code 327 Rack x 114 mm (125 ml) Boston Round bottles Code 328 Rack Pyrex 4100 (125 ml) flat-bottom flasks for rotary evaporators Code 329 Rack Pyrex (250 ml) flat-bottom flasks for rotary evaporators Code 331 Rack Sample Quantity Description GPC Sample Rack Product # x 100 mm (8 ml) tubes Code 343 Rack x 100 mm (12 ml) tubes Code 344 Rack Compatible with various GPC columns from low pressure glass to high pressure stainless steel. Automated GPC Systems ml/min Injection Volume 20 µl 5 ml Wavelength 254 or 280 nm Maximum Pressure 4,000 psi (276 bar) Mobile Rack technology enables programmed switching from drain and collect positions to completely automate the entire SPE process from condition to load, wash and elute. The GX Direct Injection Module reduces sample waste and carryover, which prevents the need to overfill the sample loop. Sample Capacity SPE: 60 (3 ml), 45 (6 ml) GPC: 80 Positive Pressure elution increases reproducibility and precision. Product #s: (Automated GX-271 GPC Clean-up System, with 112 UV Detector) (Automated GX-271 GPC Clean-up System) Product #: (Automated GX-271 GPC/SPE Clean-up System) Visit or call for more details on Gilson GPC Clean-up Systems. 31

18 GPC Clean-up Systems Gilson s Manual GPC Clean-up System is a simple and economical solution within a small footprint requiring minimal bench space. Solid Phase Extraction Systems ml/min Maximum Fractions 10 separate Injection Volume 20 µl 5 ml Wavelength 254 or 280 nm Maximum Pressure 4,000 psi (276 bar) Liquid Handler Multiple Collection Positive Pressure Elution Cartridge Sizes 1 ml 3 ml 6 ml Microplate Page Number GX-271 ASPEC 34 GX-274 ASPEC 35 Quad-Z 215 Liquid Handler for SPE 36 Manual injection valve coordinates injection time. Collection of multiple fractions from the same sample extract is done with the VALVEMATE II Valve Actuator. SPE 215 System 37 Integrate a 112 UV Detector for EPA method compatibility. Code 300 Series Racks 37 Keypad provides simple setup and control of the system for time-based fraction collection. Product #s: (Manual GPC Clean-up System, with 112 UV Detector) (Manual GPC Clean-up System) Automate solid phase extraction (SPE) processes with Gilson s complete instrument line. From 96-well extraction plates to 1, 3 or 6 ml cartridges, Gilson automated SPE solutions meet any application need. Conditioning Loading Sample Washing Eluting Analyte Interferences Visit or call for more details on Gilson SPE Systems. 33

19 Solid Phase Extraction Systems The GX ASPEC Series provides rugged and reliable automation of SPE applications utilizing 1, 3 or 6 ml cartridges. GX-27X ASPEC Series The GX-274 ASPEC is an ideal solution for mid to high-throughput sample clean-up and extraction. The GX-271 ASPEC is truly the solid solution for low to mid-throughput sample clean-up and extraction. A utomated S olid P hase E xtraction C artridges Utilizes Mobile Rack technology to completely automate the SPE process by increasing capacity and flexibility while performing both SPE and liquid handling. Cartridge Capacity 108 (1 ml), 60 (3 ml), 45 (6 ml) 98% (100 μl 25 ml) Optional safety shield available. Positive Pressure Modes syringe or gas cylinder/source 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision in comparison to vacuum based instruments. Process four SPE cartridges simultaneously for increased throughput. Mobile Rack technology enables the entire SPE process to be automated from the initial condition to the final elution and transfer while allowing access to cartridges and compounds of interest. The linear rail system permits automated method development and method optimization in which all or selected steps are collected and evaluated. On-bed transfer ports allow access to two additional large volume solvent reservoirs; incorporating a VALVEMATE II provides access to ten off-bed solvents. On-bed access to four 500 ml or 700 ml reservoirs. Built-in pressure sensing on each fluid line monitors cartridge pressure and responds to blockage by activating an error handling method. Positive Pressure elution increases reproducibility and precision due to process consistency. Choose between the 406 Single or, for more flexibility, the 406 Dual Syringe Pump for both large and small volume capabilities. Both pumps have built-in pressure sensing capabilities for detecting clogged fluid lines and monitoring cartridge pressure on each fluid line. Integrate a GX Direct Injection Module for on-line injection onto HPLC or LC/MS following SPE clean-up. Product #s: (right) (GX-271 ASPEC, with 406 Single Syringe Pump) (left) (GX-271 ASPEC, with 406 Dual Syringe Pump) (GX Direct Injection Module, 1/8, prep) (GX Direct Injection Module, 1/16, prep) (GX Direct Injection Module,1/16, analytical SS) (GX Direct Injection Module, 1/16, analytical PEEK) Product #: (GX-274 ASPEC, with two 406 Dual Syringe Pumps) Visit or call for more details on Gilson SPE Systems. 35

20 Solid Phase Extraction Systems Gilson s Quad-Z 215 Liquid Handler is a flexible and robust system that performs liquid handling tasks, on-bed filtration and Positive Pressure SPE. This liquid handler accommodates various racks maximizing flexibility for high-throughput applications. Cartridge Capacity 288 (1 ml), 144 (3 ml), 60 (6 ml) 91 x 61 x 61 cm (36 x 24 x 24 in) 98% (100 μl 25 ml) The SPE 215 System provides an automated solution for sample preparation, sample reconstitution and parallel injection onto HPLC or LC/MS systems. Additionally, the SPE 215 enables parallel clean-up, filtration and purification of combinatorial libraries and biological samples. Positive Pressure elution is achieved with Gilson s integrated Sealing Foot design. Mobile Racks automate entire SPE and liquid handling processes. Four independent probes with independent variable spacing ranging from 9 to 18 mm for access to various vessel sizes. Using Code 300 Series Racks with Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision. (see Code 300 Series Rack Capacity chart on page 37) The SPE 215 accommodates various racks offering sample preparation solutions for virtually any application. Up to eight samples can be processed every five minutes or 96 samples in an hour; a solution ideal for high-throughput sample clean-up applications. 0.98% (100 μl) 97.1% (100 μl) 97.8 x 61 x 64 cm (38.5 x 24 x 25.2 in) Product #: (SPE 215 System) Gilson s Code 300 Series Racks provide the ability to add SPE and filtration to Gilson liquid handlers including the 215 and GX Series instruments. Sample Quantity SPE Cartridge Code 300 Series Rack Capacity Collection Vessel Rack Product # 96 1 ml tabless 96-well microplate, deep Code 300 Rack ml tabless 96-well microplate, shallow Code 301 Rack ml 12 x 32 mm (2 ml) vials Code 302 Rack ml 12 x 32 mm (2 ml) vials Code 303 Rack ml 12 x 75 mm (5 ml) tubes Code 304 Rack ml 15 x 45 mm (4 ml) vials Code 305 Rack ml 12 x 75 mm (5 ml) tubes Code 306 Rack ml 15 x 45 mm (4 ml) vials Code 307 Rack Mobile Rack technology enables automated switching from drain and collect positions to accomplish the entire SPE process from condition to load, wash and elute, including selective evaluation of individual steps for method development and optimization available through N+1 movement into individual collection tubes. Sealing Cap technology enables Positive Pressure SPE ensuring better reproducibility and higher precision. Product #: (Quad-Z 215 Liquid Handler for SPE ) (see page 51 for the Gilson Rack/Instrument Compatibility Chart) Visit or call for more details on Gilson SPE Systems. 37

21 Liquid Handlers GX-281 Liquid Handler GX-271 Liquid Handler GX-274 Liquid Handler Quad-Z 215 Liquid Handler Quad-Z 215 Liquid Handler with Disposable Tips Multiple Probe 215 Liquid Handler Multiple Probes Disposable Tips Page Number < 1% (100 μl +) 98% (100 µl 25 ml) 39 < 1% (100 µl 25 ml) 98% (50 μl 25 ml) 98% (10 μl 5 ml) 0.8% (10 μl) 98.2% (10 μl) % (1 µl of DMSO) 104% (1 µl of DMSO) % (1 µl of DMSO) 113% (1 µl of DMSO) % (500 µl) 99.2% (500 µl) The GX-281 Liquid Handler is Gilson s premier liquid handling instrument. The GX-281 offers unmatched versatility and performance for liquid handling operations including tube-to-plate transfers, serial dilutions, compound reconstitution and sample filtering through the use of Code 300 Series Racks and more. The efficient speed of the GX-281 allows applications to be completed quickly and reliably. The GX Solvent Systems offer a wide range of volumes and flow rates via patented syringeless technology. Access five off-bed solvent reservoirs for bulk reagent dispensing and multiple solvent rinsing protocols. Direct request aspirating and dispensing eliminates the time spent waiting for syringes to fill. Aliquot Dilution Mixing Optional integrated bar code scanner verifies rack types and positions. < 1% (100 µl +) 87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in) Volume Delivered Measured Volume ml Transfers at 25 ml/min. 10 ml Transfers at 25 ml/min Transfers % (100 µl 25 ml) ml/min 5 µl Dispense Dispenses 215 Liquid Handler 223 Sample Changer 1.37% (50 µl of DMSO) 99.7% (50 µl of DMSO) % (10 µl) 98.2% (10 µl) 45 Gilson liquid handlers are industry renowned for rugged, reliable performance at an economical price. Whether the application requires a single probe, multiple probes, variable spacing or disposable tips, Gilson s liquid handling solutions meet all needs. Product #s: (GX-281 Liquid Handler) (GX-281 Liquid Handler, with bar code scanner) GX-281 Liquid Handler Bed Capacity Tube Diameter Sample Quantity 13 mm mm mm mm mm mm 162 Note: tubes up to 220 mm can be accommodated (see page 51 for the Gilson Rack/Instrument Compatibility Chart) Visit or call for more details on Gilson Liquid Handlers. 39

22 Liquid Handlers GX-271 Analytical Liquid Handler GX-271 Preparative Liquid Handler 98% (50 µl 25 ml) 1 µl 50 ml/min 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) GX Prep Solvent System Transfers % CV 50 µl µl 500 µl 1 ml 5 ml 10 ml 25 ml GX Prep Solvent System Transfers CV 50 µl 1.25% ± 2 µl 100 µl 1.18% ± 4 µl 500 µl 0.48% ± 7 µl 98% (10 µl 5 ml) 1 µl 5 ml/min 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) Consumes only 23" of linear bench space. GX Analytical Solvent System Transfers Flexible bed accommodates five racks and virtually any tube, vial or microplate. 1 µl % CV Ethernet based communication provides better connectivity µl 1 ml 5 ml (see GX-271 Liquid Handler/Injector page 12) Volume Volume The GX-271 Liquid Handler, Gilson s newest instrument, offers a medium capacity with a small footprint. The GX-271 models are economical and robust liquid handlers for reformatting, reagent dispensing and performing serial dilutions. Their modular design allows for future expansion by adding injection and fraction collection capabilities. Volume Liquid Level Detection allows for detecting a variety of solvents as low as 20 µl in a 384-well microplate. GX Analytical Solvent System Transfers GX-271 Preparative and Analytical Liquid Handler Bed Capacity Volume CV 1 µl 11.98% ± 300 nl 10 µl 4.5% ± 1 µl Tube Diameter Sample Quantity 1 ml 0.18% ± 20 µl 10 mm ml 0.2% ± 100 µl 13 mm ml 0.14% ± 5 µl 5 ml 0.09% ± 16 µl 15 mm ml 0.07% ± 105 µl 16 mm ml 0.02% ± 46 µl 17 mm mm mm 70 Jet Wash Rinse technology provides flowing rinse for fast and efficient rinsing protocols utilizing up to two separate flowing rinse solvents. Gilson s GX Solvent System, available in an analytical or prep model, features a dynamic range for volumes and flow rates and can access five off-bed solvents. T he GX-271 Liquid Handler is available without a pump or GX Solvent System, allowing the instrument to be configured with a 402 Syringe Pump. Note: tubes up to 200 mm can be accommodated (see page 51 for the Gilson Rack/Instrument Compatibility Chart) Product #s: (GX-271 Preparative Liquid Handler) (GX-271 Analytical Liquid Handler) (GX-271 Liquid Handler without pump) Visit or call for more details on Gilson Liquid Handlers. 0902_Inside32_45.indd /24/07 10:27:48 AM

23 Liquid Handlers The GX-274 Liquid Handler is a mid-throughput four probe instrument capable of processing up to four samples (at 18 mm fixed spacing) in parallel, increasing the efficiency of liquid handling applications. The GX-274 Liquid Handler provides an economical option with a small footprint for achieving increased throughput in a variety of applications. The GX-274 is ideal for bulk reagent dispensing when used in conjunction with a Minipuls Evolution or Minipuls 3 Peristaltic Pump or for tube-to-plate transfers using 402 Syringe Pumps. Access four on-bed solvent reservoirs without consuming a rack position. 98.2% (10 µl) < 0.8% (10 µl +) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) Flexible rack system accommodates tubes, vials and microplates. Optional flowing rinse station with solvent ports provides active probe rinsing from off-bed solvents. The Quad-Z 215 Liquid Handler was designed based on the robust Gilson 215 Liquid Handler. This instrument accommodates numerous racks for maximum flexibility of sample transfer between tubes, vials and microplates. Gilson s Quad-Z 215 Liquid Handler can be configured with a Disposable Tips option, a requirement in molecular biology and biochemistry applications. Disposable tips prevent crosscontamination, which saves time by eliminating rinsing steps. This system includes on-bed storage of tips, as well as a chute for collection of used tips. Flexible configuration enables use of fixed probes and 10 μl, 200 μl and 1 ml disposable tips. 104% (1 μl of DMSO) Injection Carryover < 0.05% 113% (1 μl of DMSO) 91 x 61 x 61 cm (36 x 24 x 24 in) liquid handling: 2.1% (1 μl of DMSO) injection: < 2.0% (500 μl syringe, partial loop); < 5.0% (500 μl syringe, total loop) Disposable Tips Option Injection Volume 5 μl 5 ml 0.8% (10 μl +); 4.3% (1 μl of DMSO) A VALVEMATE II can be incorporated enabling access to ten additional off-bed solvents. Integrating two 402 Dual Syringe Pumps provides four independently controlled syringes from 100 μl to 25 ml. For access to the various vessel sizes, the four independent probe variable spacing ranges from 9 to 18 mm. Product #s: (GX-274 Liquid Handler) F (402 Dual Syringe Pump) Product #s: (Quad-Z Liquid Handler) (Quad-Z 215 Adapter Kit for Disposable Tips) (849 Multiple Injection Module) F (402 Dual Syringe Pump) Visit or call for more details on Gilson Liquid Handlers. 43

24 Liquid Handlers Select either a four or eight probe configuration depending on application and throughput requirements. Ideal solution for plate-to-plate transfers. The Multiple Probe 215 is a high-throughput automated liquid handler capable of tasks such as sample transfers, dilutions, mixing, reagent addition and microplate reformatting. The Multiple Probe 215 features patented Dual Rail Syringe Drive technology, accurate use of four or eight syringes and accommodates probes that fit all application requirements. The 223 Sample Changer is a programmable sampler for automated sample preparation and transfer. It provides a cost-effective solution for protocols such as serial dilutions, additions, sampling into vials, timed reactions and tube-to-tube transfers. The 223 Sample Changer is ideal for automated transfer of samples in analytical techniques like FIA and spectroscopy (UV/VIS, AA and ICP). 0.8% (10 μl) 98.2% (10 μl) 53.5 x 43.7 x 60 cm (21.1 x 17.2 x 24 in) Cross-contamination is minimized by adjusting rinse volumes and using liquid level sensing. Carryover < 1 ppb 0.57% (500 µl) 99.2% (500 µl) Optional flow-through rinse station, available on the left and/or right of the support bar, reduces cross-contamination. Choose between an optional 402 Syringe Pump, Minipuls Evolution or Minipuls 3 Peristaltic Pump to add aspirating and dispensing flexibility x 61 x 56 cm (36 x 24 x 22 in) Product #: (Multiple Probe 215 Liquid Handler) The 215 Liquid Handler is a laboratory tested workhorse for performing dilutions, aliquoting solutions, mixing, plate reformatting, reconstituting of samples and more. The 215 Liquid Handler has a punch strength for piercing thick or thin film septa and microplate mats that is distinguished throughout the liquid handling industry. Accommodates a wide variety of probes as well as standard and custom racks. (see page 51 for the Gilson Rack/ Instrument Compatibility Chart) Flexible open bed design allows for a variety of rack options including tubes, vials, microplates and Peltier control. 1.37% (50 µl) Carryover < 1 ppb 99.7% (50 µl) Product #s: (215 Liquid Handler) (215 Liquid Handler without pump) 91 x 61 x 56 cm (36 x 24 x 22 in) Product #: (223 Sample Changer) Visit or call for more details on Gilson Liquid Handlers. 45

25 Accessories Switching Valve Mixing Racks Heating/Cooling Page Number The VALVEMATE II Valve Actuator automates HPLC and LC techniques by increasing the system flexibility to save time, increase throughput and extend column life. It is designed to facilitate both solvent selection and column switching. The VALVEMATE II increases flexibility of HPLC systems allowing for multiple valve and multiple column configurations. 8.3 x 15.6 x 9.8 cm (3.3 x 6.2 x 3.9 in) Valve Switching Speed < 180 ms port-to-port (depending on valve) VALVEMATE II Valve Actuator Orbital Shaker 48 RackCount System 818 AutoMix High Pressure Valves: Analytical 2-position, 6-port, stainless steel (0.016 ID ports) Analytical 2-position, 10-port, stainless steel (0.016 ID ports) Preparative 2-position, 6-port, stainless steel (0.030 ID ports) Preparative 2-position, 10-port, stainless steel (0.030 ID ports) Preparative 2-position, 6-port, PPS (0.030 ID ports) Preparative 2-position, 10-port, PPS (0.030 ID ports) Multi-position, 6-port, stainless steel (0.016 ID ports) Multi-position, 6-port, stainless steel (0.030 ID ports) Multi-position, 6-port, PEEK (0.016 ID ports) Multi-position, 10-port, stainless steel (0.016 ID ports) Multi-position, 10-port, PEEK (0.016 ID ports) Low Pressure Valves: Multi-position, 6-port, PPS (0.030 ID ports) Multi-position, 6-port, PPS (0.060 ID ports) Multi-position, 8-port, PPS (0.060 ID ports) 6 Column Plumbing Schematic 852/853/854 Peltier Racks 40ºC 4ºC /542 Peltier Racks Complementing robust and flexible instruments, Gilson s accessories optimize instrumentation to fit application requirements for the ultimate solution. 40ºC 4ºC 50 Multi-directional keypad control with valve position LED display. Contact closure control for home and switch valve position. Fast valve switching times minimize pressure spikes and carryover. Product #: AB (VALVEMATE II Valve Actuator) Visit or call for more details on Gilson Accessories. 47

26 Accessories The Gilson Orbital Shaker is specifically designed to satisfy liquid handling needs for on-line or off-line vortexing and mixing. It is a fully functional, stand-alone instrument equipped with a single-stepper motor. Designed for Gilson s 215 Series instruments, the 818 AutoMix provides fully automated, software-driven mixing and sampling. Typical applications that use the AutoMix include mixing whole blood samples, immiscible liquids and more. Mix an entire rack of samples with oscillating mixing. Mixing Speed/ 10 rpm: ± 0.5 rpm 20 rpm: ± 1 rpm Rack Capacity (1) Code 200 Series Rack 22.9 x 50.8 x 16.5 cm (9 x 20 x 6.5 in) Circular Rotational Speed rpm Rack Capacity (1) Code 200 Series Rack 11.8 x 31.6 x 4.7 cm (4.65 x x 1.85 in) The Orbital Shaker can be placed on the bed of the GX-281 or 215 Series instruments using the optional mounting plate kits. Product #: (818 AutoMix) Product #: (Orbital Shaker) Gilson s RackCount System, numbered overlays that fit securely on the top of racks, provides a quick and effortless bed location reference for specific samples or collected fractions on a Gilson liquid handler. RackCount overlays can be used with new rack purchases or added to existing Gilson Code 200 Series Racks. They have sequentially numbered fraction collection positions for five complete rack positions and one position for sample locations. The numbering patterns are solvent resistant and compatible with most normal and reversed-phase solvents. Gilson offers flexible, accurate and programmable racks for heating and cooling valuable samples; cooling to reduce evaporation and maintain sample integrity, heating to facilitate reactions and improve kinetics. Gilson offers a variety of standard and custom rack configurations. Product #s: (far left) (852 Peltier Rack) (center left) (853 Peltier Rack) (center right) (854 Peltier Rack) (far right) (Peltier Controller) Up to five racks can be controlled from a single programmable Peltier controller integrated with a system or as a stand-alone unit. Precise temperature control from 4 to 40 C. Rack Capacity 852: (2) 96-well microplates, shallow 853: (96) 12 x 32 mm (2 ml) flat-bottom vials 854: (2) 96-well microplates, deep 11.9 x 33.8 x 16.8 cm (4.7 x 13.3 x 6.6 in) Visit or call for more details on Gilson Accessories. 49

27 Accessories Reference Gilson Peltier Racks are individually controlled to cool and heat microplates on the bed of a Gilson liquid handler. The racks provide accurate temperature control from 4 to 40 C. Product #s: (left) (242 Peltier Rack) (center) (542 Peltier Rack) (right) (215 Peltier Controller) Gilson offers thousands of standard and custom racks to meet the needs of any and all applications. A variety of vessels can be accommodated including tubes, vials, microplates and microchips. The combination of a 542 and a 242 Peltier Rack can accommodate over 1,000 samples on a 215 Liquid Handler. Rack Capacity 242: (2) 96-well microplates, shallow 542: (10) 96-well microplates, shallow A single Peltier controller is able to power a 542 and a 242 Peltier Rack or up to five 242 Peltier Racks. 242: 11.4 x 30.5 x 12.4 cm (4.5 x 12 x 4.9 in) 542: 50.1 x 33.6 x 11.4 cm (20.1 x 13.3 x 4.5 in) Rack Code Gilson Rack/Instrument Compatibility Chart Rack Product # Capacity Description Microplate Vessel Capacity (ml) x 32 mm (2 ml) vials X X X x 65 mm (6 ml) or 13 x 100 mm (9 ml) tubes 6 or or 100 X x 50 mm (1 ml) tubes X x 75 mm (5 ml) or 12 x 100 mm (8 ml) tubes 5 or or 100 X well microplate, shallow flat-bottom X 1 X x 32 mm (0.3 ml) vials X X x 40 mm (0.7 ml) vials X X x 100 mm (9 ml) tubes X x 75 mm (5 ml) or 12 x 100 mm (8 ml) tubes 5 or or 100 X well microplate, shallow X 1 X well microplates, shallow X 4 X 16D D 4-96-well microplates, deep X 4 X , 48 or 96-well microplates, Whatman Deep Polyfiltronics Plates Vessel Quantity Vessel Diameter Vessel Height GX-281 LH/Inj X 8 X x 100 mm (4.5 ml) tubes X X X X X X X X X X x 100 mm (9 ml) tubes X X X X X X X X X X x 150 mm (25 ml) tubes X X X X X X X X 22U x 100 mm (4.5 ml) to 18 x 180 mm (32 ml) tubes 4.5 to to to 180 X X X X X X X X x 55 mm (6.8 ml) scintillation vials X X X X X X X X X X 23W x 45 mm (4 ml) WISP vials X X X X X X X X x 57 mm (20 ml) scintillation vials X X X X X X X X X X x 75 mm (3.5 ml) tubes X X X X X X X X X X x 75 mm (5 ml) or 13 x 75 mm (7 ml) tubes 5 or or X X X X X X X X X X 29LE (1.5 ml) Eppendorf vials X X X X X X 29SE (0.5 ml) Eppendorf vials X X X X X X x 32 mm (2 ml) vials X X X X 30P P x 32 mm (2 ml ) vials X X x 40 mm (0.7 ml) vials X X X 31P P x 40 mm (0.7 ml) vials X x 65 mm (6 ml) or 13 x 100 mm (9 ml) tubes 6 or or 100 X X X X x 57 mm (20 ml) scintillation vials X X X X 33P P x 57 mm (20 ml) scintillation vials X X GX-271 LH/Inj 215 LH/Inj Multiple Probe 215 LH/Inj GX-274 LH GX-271 LH 223 Sample Changer Quad-Z 215 LH Quad-Z 215 (Disposable Tips) GX-274 ASPEC GX-271 ASPEC SPE 215 prepfc FC 204 FC 203B Orbital Shaker Visit or call for more details on Gilson Instruments and Software. 51

28 Reference Gilson Rack/Instrument Compatibility Chart continued Rack Code Rack Product # Capacity Description Microplate x 45 mm (4 ml) WISP vials X X 34P P x 45 mm (4 ml) WISP vials X 35P P x 32 mm (2 ml) vials x 60 mm (20 ml) vial 36P P x 41 mm (0.7 ml) vials x 60 mm (20 ml) vial Vessel Capacity (ml) Vessel Quantity Vessel Diameter Vessel Height GX-281 LH/Inj GX-271 LH/Inj 215 LH/Inj Multiple Probe 215 LH/Inj GX-274 LH GX-271 LH X X x 32 mm (2 ml) vials x 60 mm (20 ml) vial X well microplates, shallow X 2 X Kimax or Pyrex (500 ml) bottles X Kimax or Pyrex (1000 ml) bottles X x 100 mm (9 ml) tubes X X X X x 100 mm (12 ml) tubes X X X X (3 ml) DECs 3 20 X X X X X (6 ml) DECs 6 8 X X X X X x 100 mm (9 ml) Vacutainer tubes X X X X X X X X X well microplates (1.5 ml) microcentrifuge tubes x 100 mm (9 ml) tubes 201H H 2-96-well microplates, w/covers (1.5 ml) microcentrifuge tubes x 100 mm (9 ml) tubes x 47 mm (1.5 ml) microcentrifuge tubes or (2.5 ml) Vacutainer tubes X X X X X X X X X X X X X X X X X X X X X X X X X X 1.5 or X X X X X X X x 64 mm (3 ml) tubes X X X X X X X X X x 57 mm (20 ml) scintillation vials X X X X X X X X X 204F F x 57 mm (20 ml) scintillation vials X X well microplates, deep (1.5 ml) Eppendorf vials x 100 mm (9 ml) tubes 205H H 2-96-well microplates, deep w/covers (1.5 ml) Eppendorf vials x 100 mm (9 ml) tubes X X X X X X X X X X X X X X X X X X X X X X X X X X x 75 mm (7 ml) Vacutainer tubes X X X X X X X X X x 100 mm (12 ml) Vacutainer tubes X X X X X X X X X x 150 mm (25 ml) tubes X X X X X X X X x 32 mm (2 ml) vials X X X X X X X X X x 75 mm (10 ml) Vacutainer tubes X X X X X X X X X x 113 mm (125 ml) bottles X X X X X X X X X 211F F 8-48 x 113 mm (125 ml) bottles, w/funnel drain X X 211H H 9-48 x 113 mm (125 ml) bottles, w/cover X X X X X X X X X x 100 mm (9 ml) tubes x 75 mm (7 ml) Vacutainer tubes X X X X X X X X X 223 Sample Changer Quad-Z 215 LH Quad-Z 215 (Disposable Tips) GX-274 ASPEC GX-271 ASPEC SPE 215 prepfc FC 204 FC 203B Orbital Shaker Rack Code Rack Product # Capacity Description x100 mm (12 ml) tubes x 75 mm (10 ml) Vacutainer tubes x 47 mm (2.5 ml) tubes x 64 mm (3 ml) Vacutainer tubes Microplate Vessel Capacity (ml) Vessel Quantity Vessel Diameter Vessel Height GX-281 LH/Inj GX-271 LH/Inj 215 LH/Inj X X X X X X X X Multiple Probe 215 LH/Inj GX-274 LH GX-271 LH 223 Sample Changer Quad-Z 215 LH Quad-Z 215 (Disposable Tips) GX-274 ASPEC GX-271 ASPEC SPE 215 prepfc FC 204 FC 203B X X X X X X X X x 45 mm (4 ml) WISP vials X X X X X X X X x 75 mm (4 ml) tubes X X X X X X X X X X X X well microplates, shallow or deep x 75 mm (4 ml) tubes; also holds Beckman modular reservoirs X X X X X X X X X X X X x 95 mm (40 ml) vials X X X X X X X X X x 62 mm (7 ml) vials X X X X X X X x 115 mm (50 ml) conical bottom tubes X X X X X X X X X 222F F x 115 mm (50 ml) conical bottom tubes, w/ funnel drain X X x 100 mm (12 ml) tubes, w/screw caps X X X X X X X x 41 mm (27 ml) vials X X X X X X X x 150 mm (45 ml) vials X X X X X X X x 45 mm (4 ml) WISP vials X X X X X X X (500 ml or 700 ml) bottles 500 or X X X X X X X X X X X Tipack boxes (10 µl or 200 µl tips) 2 X Tower Pack boxes (10 µl or 200 µl tips) 2 X well microplates X 2 X X X X X X X X X X X X x 200 mm (70 ml) tubes X X X X X X well microplates, shallow X 2 X X X X X X X X X X (1 ml) cartridges well microplate, deep (1 ml) cartridges well microplate, shallow (1 ml) cartridges x 32 mm (2 ml) vials (3 ml) cartridges x 32 mm (2 ml) vials X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X (3 ml) cartridges x 75 mm (5 ml) tubes X X X X X X X X X (3 ml) cartridges x 45 mm (4 ml) vials X X X X X X X X X (6 ml) cartridges x 75 mm (5 ml) tubes X X X X X X (6 ml) cartridges x 45 mm (4 ml) vials X X X X X X x 32 mm (2 ml) vials X X X x 150 mm (25 ml) tubes X X X X X (40 ml) scintillation vials X X X X X Orbital Shaker Visit or call for more details on Gilson Instruments and Software. 53

29 Reference Rack Code Gilson Rack/Instrument Compatibility Chart continued Rack Product # Capacity Description Microplate Vessel Capacity (ml) x 45 mm (4 ml) WISP vials X X X X X X well microplates, shallow or deep X 2 X X X X X well microplates, shallow or deep X 4 X X X X X x 32 mm (2 ml) vials X X X X X x 75 mm (3.5 ml) tubes X X X X X x 100 mm (9 ml) tubes X X X X X x 150 mm (20 ml) tubes X X X X X (1 ml) DECs 1 36 X X X X (3 ml) DECs 3 20 X X X X (6 ml) DECs 6 15 X X x 32 mm (2 ml) vials (1 - HP 1100LC tray) X X well microplates, shallow or deep X 10 X X X X X 505H H well microplates, shallow or deep w/covers X 10 X X X X X well microplates, shallow or deep X 17 X X well microplates, shallow X 10 X X X X (1 ml) tabbed SPE cartridges 1 48 X (1 ml) tabless SPE cartridges 1 96 X (3 ml) tabless SPE cartridges 3 48 X well wide SPE plate, compatible with porvair-style plates such as Varian & Waters 1-96-well narrow SPE plate, compatible with plates such as 3M & Ansys Spec well Becton Dickinson Falcon "U-bottom" microplates, shallow Vessel Quantity Vessel Diameter Vessel Height GX-281 LH/Inj GX-271 LH/Inj 215 LH/Inj 1 X 1 X X 2 X X X X X X X X x 32 mm (2 ml) vials, flat-bottom X X X X X X well Beckman or Ritter microplates, deep X 2 X X X X X X X X VR SPE extraction plates: Waters OASIS, 3M Empore and Varian VersaPlate M Maldi Rack, 1 - Bruker MALDI Plate X Gilson Probe Product Number Identification Gilson probes are laser engraved with their product number on the collar of each probe providing a quick and effortless identification reference for re-ordering and support. Multiple Probe 215 LH/Inj GX-274 LH GX-271 LH 223 Sample Changer Quad-Z 215 LH X Quad-Z 215 (Disposable Tips) GX-274 ASPEC GX-271 ASPEC SPE 215 prepfc FC 204 FC 203B Orbital Shaker Probes Gilson offers many different types of probes, probe tips and coatings to meet any and all application needs providing solutions for all liquid handling requirements. Tip Design Description Features Flat The tip is cut perpendicular to the length of the probe. Aspirates the last drop of liquid Does not pierce septum No injection Constricted The tip of the probe has a smaller outer diameter than the rest of the shaft. Constricted-tip probes accommodate unique tip designs optimizing every application. Compatible with injection ports Beveled The tip is cut at an angle, leaving a bevel. Sharp tip enables septum-piercing Compatible with injection ports Spring Loaded Low volume, touch-off dispensing. Ideal for MALDI spotting applications Spring loaded probe removes any potential of damaging spotting surface Vented Side-entry Deflected Two styles: 1. A shallow groove cut in the probe shaft, above the tip (shown). 2. Two probes are assembled concentrically, creating two separate channels, the inner channel for liquid and the outer channel for gas. Vented probes may have different tip designs. The liquid flow path opens on the side of the probe, not at the tip. Based on the beveled tip design, the probe tip is curved and very sharp. Sharp-tip enables septum-piercing Vent allows pressures inside and outside a sealed vessel to equilibrate as liquid is dispensed or aspirated Recommended for thick septa Prevents coring of septa Compatible with injection ports Not recommended with limited volume Sharp-tip enables septum-piercing Tip pushes septa out of the way as it pierces to prevent coring Conical Designed for PEEK injection ports. Capable of piercing thin septa Aspirates the last drop of liquid Visit or call for more details on Gilson Instruments and Software. 55

30 Reference Gilson Probe Selection Guide Product # Septum-piercing Tip Shape Material 175 mm Z-arm Dimensions (mm, L x OD x ID) Injection Compatibility Constricted, flat Stainless steel 269 x 1.8 x 1.4 GX Z-Injection Module Constricted, flat Stainless steel 269 x 1.3 x Beveled, level-sensing Stainless steel 269 x 1.5 x 1.1 Tip: 1.6 x 1.2 x 0.8 Port # ) 849 (requires Injection Port # ) GX Direct Injection Module (requires Injection Port # ) Port # ) 849 # Seal # GX Direct Injection Module (requires Injection Port # ) X Beveled Stainless steel 323 x 1.8 x SPE Micro septum Conical, micro-volume Beveled, levelsensing Stainless steel 269 x 2.4 x Stainless steel 269 x 1.5 x 0.4 Tip: Con Bev Micro 0.6 Port #270728) Flat, level-sensing Stainless steel 269 x 1.8 x 1.4 GX Z-Injection Module Beveled, levelsensing Stainless steel 269 x 1.5 x Beveled Stainless steel 269 x 1.3 x Flat Inert Gas Probe Stainless steel, Teflon coated (plastic tip) x 2.7 x 0.8 Tip: 10 Port #295640) 849 # GX Direct Injection Module (requires Injection Port # ) Port # ) 849 (requires Injection Port Bar # ) GX Z-Injection Module X Beveled Stainless steel 317 x 1.8 x 0.8 GX Z-Injection Module Flat Stainless steel x 2.7 x 0.8 GX Z-Injection Module X Beveled Stainless steel 125 mm Z-arm 269 x 2 x 0.8 Tip: 1.5 OD x X Side-entry Stainless steel 274 x 1.8 x X Side-entry Stainless steel 274 x 1.8 x X Side-entry Stainless steel x 1.5 x X Side-entry Stainless steel 273 x 1.8 x 0.8 Port # ) GX Direct Injection Module (requires Injection Port # ) Port # ) GX Z-Injection Module Port # ) GX Z-Injection Module Port # ) GX Z-Injection Module Port # ) GX Z-Injection Module Instrument Compatibility GX-271, GX-274 GX-281, 215, 215 SW, Multiple Probe , 215 SW, Multiple Probe 215, Quad-Z , 215 SW, Multiple Probe 215, Quad-Z 215 Quad-Z , 215 SW, Quad-Z 215 GX-271 ASPEC, GX-274 ASPEC, 215, 215 SW, Quad-Z 215 GX-271, GX-274, 215, 215 SW, Multiple Probe 215, Quad-Z , 215 SW Product # Septum-piercing Tip Shape Material 125 mm Z-arm continued X Deflected X Deflected Stainless steel, Teflon interior Stainless steel, Teflon interior Dimensions (mm, L x OD x ID) Injection Compatibility 274 x 1.8 x 0.8 GX Z-Injection Module 274 x 1.8 x 0.8 GX Z-Injection Module X Deflected Stainless steel 274 x 1.8 x 0.8 GX Z-Injection Module X Deflected Stainless steel 274 x 1.8 x 0.8 GX Z-Injection Module Micro septum Conical, microvolume Constricted, beveled Stainless steel 220 x 2.1 x Stainless steel 220 x 1.5 x 0.4 Tip: 10 x 0.7 x X Beveled Stainless steel 221 x 1.5 x Constricted, flat Stainless steel 220 x 1.3 x Beveled Stainless steel x 1.5 x Constricted, flat Stainless steel 221 x 1.5 x X Beveled Stainless steel 221 x 2 x 0.8 Tip: 2.1 x 1.5 x X Beveled Stainless steel 221 x 1.5 x mm Z-arm Flat Stainless steel 51 x 0.7 x 0.4 Port # ) 849 # Port # ) GX Direct Injection Module (requires Injection Port # ) Port # ) 849 (requires Injection Port Bar # ) GX Direct Injection Module (requires Injection Port # ) Port # ) 849 # GX Direct Injection Module (requires Injection Port # ) Port # ) 849 # GX Direct Injection Module (requires Injection Port # ) Port # ) GX Direct Injection Module (requires Injection Port # ) Port # ) 849 # GX Direct Injection Module (requires Injection Port # ) X Beveled Stainless steel 103 x 0.7 x 0.4 Requires Injection Port # XL Constricted, flat Stainless steel 93.5 x 1.5 x X Beveled Stainless steel 95 x 0.7 x X Beveled Titanium 95 x 0.7 x mm Z-arm Beveled Stainless steel x 1.5 x 1.1 Requires Injection Port # & Probe Sleeve # Requires Injection Port # Requires Sleeve # X Side-entry Stainless steel x 1.5 x 1.1 Requires Injection Port # Instrument Compatibility , Multiple Probe 215 GX-271 ASPEC, GX-274 ASPEC, , Multiple Probe , Multiple Probe , Multiple Probe XL/233 XL 215, Multiple Probe 215 (183 mm arm) 215, Multiple Probe 215 (183 mm arm), GX-271 GPC Systems GX-271, GX-274, 223, 231 XL 223, 231 XL/232 XL/ 233 XL 223, 231 XL/232 XL/ 233 XL 223, 221 XL, 231 XL/232 XL/233 XL, ASTED XL Tapered, flat Stainless steel x 1.5 x , 221 XL Visit or call for more details on Gilson Instruments and Software. 57

31 Reference Gilson Probe Selection Guide continued Product # Septum-piercing Tip Shape Material 123 mm Z-arm continued Flat Flat Teflon coated (plastic tip) Teflon coated (plastic tip) Dimensions (mm, L x OD x ID) x 0.9 (18 g) Tip: x 0.9 (22 g) Tip: Tapered, beveled Stainless steel 162 x 0.7 x X Beveled Stainless steel 162 x 0.7 x X Beveled Titanium 162 x 0.7 x 0.42 Injection Compatibility Requires Injection Port # & Probe Sleeve # Requires Injection Port # & Probe Sleeve # Requires Injection Port # & Probe Sleeve # X Beveled Stainless steel 162 x 0.7 x 0.4 Requires Injection Port # Tapered, beveled Stainless steel x 1.5 x 1.1 Requires Injection Port # Double probe kit X Beveled Stainless steel Flat 183 mm Z-arm Beveled, levelsensing Teflon coated (plastic tip) probe 1: x 1.5 x 1.1 probe 2: x 2.5 x x 0.9 Tip: 10 Stainless steel x 1.5 x 1.1 Port # ) 232 XL/233 XL (requires Injection Port # ) Instrument Compatibility , 231 XL/232 XL/233 XL, ASTED XL 223, 231 XL/232 XL/233 XL, ASTED XL 231 XL, ASTED XL 223, 231 XL/232 XL/233 XL, ASTED XL 223, 231 XL/232 XL/233 XL, ASTED XL 215, 223, 232 XL/233 XL X Side-entry Stainless steel x 1.5 x 1.1 Requires Injection Port # , 232 XL/233 X Constricted, flat, level-sensing Stainless steel 221 x 1.5 x 1.1 Tip: 2 x 1.1 x 0.4 Port # ) 849 # # , Multiple Probe 215, 223, 232 XL/233 XL Flat Teflon coated x XL/233 XL 210 mm Z-arm X Beveled Stainless steel X Beveled Stainless steel (coated) 304 x 2 x 0.8 Tip: 2.1 x 1.5 x x 2 x 0.8 Tip: 2.1 x 1.5 x X Beveled Stainless steel 304 x 1.5 x X Beveled Stainless steel (coated) x 1.5 x X Beveled Stainless steel 304 x 1.5 x Beveled Stainless steel 304 x 1.5 x 1.1 GX Direct Injection Module (requires Injection Port # ) GX Direct Injection Module (requires Injection Port # ) GX Direct Injection Module (requires Injection Port # ) GX Direct Injection Module (requires Injection Port # ) GX Direct Injection Module (requires Injection Port # ) GX Direct Injection Module (requires Injection Port # ) GX-281 GX-281 GX-281 GX-281 GX-281 GX Flat Stainless steel 304 x 2.4 x 1.8 GX Flat Stainless steel 304 x 1.8 x 1.4 GX-281 1A 1 H Li Na K Rb Cs Fr (223) 2A 4 Be Mg Ca Sr Ba Ra B 21 Sc Y * ** *Lanthanides **Actinides 4B 22 Ti Zr Hf Rf (261) 57 La Ac Periodic Table of the Elements 5B 23 V Nb Ta Ha (262) 58 Ce Th B 24 Cr Mo W Unh (263) 59 Pr Pa B 25 Mn Tc (98) 75 Re Uns (262) 60 Nd U Fe Ru Os Hs (277) 61 Pm (145) 93 Np B 27 Co Rh Ir Sm Pu (244) 28 Ni Pd Pt Eu Am (243) 1B 29 Cu Ag Au Gd Cm (247) 2B 30 Zn Cd Hg Tb Bk (247) 3A 5 B Al Ga In Tl Dy Cf (251) 6 C Si Ge Sn Pb Ho Es (252) 7 N P As Sb Bi Er Fm (257) 8 O S Se Te Po (209) 69 Tm Md (258) 9 F Cl Br I At (210) 70 Yb No (259) Undiscovered Synthetic From Decay Primordial 2 He Ne Ar Kr Xe Rn (222) 109 Une 110 Ds 111 Rg 112 Uub 113 Uut 114 Uuq 115 Uup 116 Uuh 117 Uus 118 Uuo Not Yet (276) (281) (280) (285) (284) (289) (288) (291) Observed (294) Flow Cell Selection Chart for Optimizing Sensitivity on 15X Series Detectors Selecting the correct flow cell will optimize sensitivity and peak shape. The following chart pairs flow cells with the appropriate column, flow rate and sample concentration to help choose the correct flow cell for the application. Capillary Flow Cell Micro-bore Flow Cell Analytical Flow Cell Semi-preparative Flow Cell Preparative Flow Cell Product # Internal Volume 35 nl 1.5 µl 12 µl 0.7 µl 0.16 µl Pathlength 8 mm 2 mm 5 mm 0.2 mm 0.05 mm Column Size µm 1 2 mm 4 mm 10 mm 4A 5A 6A 7A 20 mm > 40 mm 8A 71 Lu Lr (260) 1 10 µl/min µl/min 1 15 ml/min ml/min ml/min Purify ug - 5 mg Purify mg Purify 300 mg - 15 g Visit or call for more details on Gilson Instruments and Software. 59

32 Reference ph of Acids and Bases ph of Common Acids ph of Common Bases Acids (ph < 7) Molarity ph Bases (ph > 7) Molarity ph Acetic 1 N 2.4 Ammonia 1 N 11.6 Acetic 0.1 N 2.9 Ammonia 0.1 N 11.1 Acetic 0.01 N 3.4 Ammonia 0.01 N 10.6 Alum 0.1 N 3.2 Barbital Sodium 0.1 N 9.4 Arsenious Saturated 5 Borax 0.01 N 9.2 Benzoic 0.1 N 3 Calcium Carbonate Saturated 9.4 Boric 0.1 N 5.3 Calcium Hydroxide Saturated 12.4 Carbonic Saturated 3.8 Ferrous Hydroxide Saturated 9.5 Citric 0.1 N 2.1 Lime Saturated 12.4 Formic 0.1 N 2.3 Magnesia Saturated 10.5 Hydrochloric 1 N 0.1 Potassium Acetate 0.1 N 9.7 Hydrochloric 0.1 N 1 Potassium Bicarbonate 0.1 N 8.2 Hydrochloric 0.01 N 2 Potassium Carbonate Hydrocyanic 0.1 N 5.1 Potassium Cyanide 0.1 N 11 Hydrogen Sulfide 0.1 N 4.1 Potassium Hydroxide 1 N 14 Lactic 0.1 N 2.4 Potassium Hydroxide 0.1 N 13 Malic 0.1 N 2.2 Potassium Hydroxide 0.01 N 12 Nitric 0.1 N 1 Sodium Acetate 0.1 N 8.9 Orthophosphoric 0.1 N 1.5 Sodium Benzoate 0.1 N 8 Oxalic 0.1 N 1.3 Sodium Bicarbonate 0.1 N 8.4 Succinic 0.1 N 2.7 Sodium Carbonate 0.1 N 11.6 Salicylic Saturated 2.4 Sodium Hydroxide 1 N 14 Sulfuric 1 N 0.3 Sodium Hydroxide 0.1 N 13 Sulfuric 0.1 N 1.2 Sodium Hydroxide 0.01 N 12 Sulfuric 0.01 N 2.1 Sodium Metasilicate 0.1 N 12.6 Sulfurous 0.1 N 1.5 Sodium Sesquicarbonate 0.1 N 10.1 Tartaric 0.1 N 2 Trisodium Phosphate 0.1 N 12 Trichloracetic 0.1 N 1.2 Column Sizes and Capacities Internal Diameter (mm) Typical Injection Volume (µl) Load Capacity (mg) Typical s (ml/min) , , , Maximum UV Absorbance for Commonly Used Solvents Operating at a wavelength near or below a mobile phase solvent s maximum UV absorbancy increases baseline noise due to the UV response of the solvent. Refer to the tables below to optimize chromatographic conditions. Solvent Table 1 Maximum UV Absorbance (nm) 1-Nitropropane Butoxyethanol 220 Acetone 330 Acetonitrile 190 Amyl Alcohol 210 Amyl Chloride 225 Benzene 280 Butanol Butanol n-butyl Chloride 220 Carbon Disulfide 380 Carbon Tetrachloride 265 Chloroform 245 Clorobenzene 287 Cyclohexane 200 Cyclopentane 200 Diethyl Amine 275 Dimethyl Sulfoxide 268 Dioxane 215 Ethanol 210 Ethyl Acetate 256 Ethyl Ether 220 Ethyl Sulfide 290 Ethylene Dichloride 230 Ethylene Glycol 210 Heptane 200 Hexane 195 Isooctane 215 Isopropanol 205 Isopropyl Chloride 225 Isopropyl Ether 220 Methanol 205 Methyl Acetate 260 Methyl Ethyl Ketone 330 Methyl Isobutyl Ketone 334 Methylene Chloride 233 n-pentane 190 n-propanol 210 n-propyl Chloride 225 Nitromethane 380 Petroleum Ether 210 Pyridine 330 Tetrahydrofuran 230 Toluene 285 Xylene 290 Mobile Phase Conditioners & Buffers Table 2 Maximum UV Absorbance (nm) Acetic Acid, 1% 230 Ammonium Acetate, 10 mm Ammonium Bicarbonate, 10 mm Diammonium Phosphate, 50 mm EDTA, Disodium, 1 mm 190 HEPES, 10 mm, ph Hydrochloric acid, 0.1% 190 MES, 10 mm, ph Potassium Phosphate Monobasic, 10 mm Dibasic, 10 mm Sodium Acetate, 10 mm 205 Sodium Chloride, 1M 207 Sodium Citrate, 10 mm 225 Sodium Dodecyl Sulfate 190 Sodium Formate 200 Triethylamine, 1% 235 Trifluoracetic Acid, 0.1% 190 TRIS HCl, 20 mm ph 7.0 ph Visit or call for more details on Gilson Instruments and Software. 61

33 Reference Optimizing Preparative HPLC There are numerous parameters and techniques to optimize preparative HPLC. These reference tables provide important physical parameters of the tubing and injection valve structure; optimization will improve chromatography and percent recovery of collected fractions. Table 1: 33X Series pump tubing (PEEK)* At high-flow rates tubing can cause Tubing ID (in) (ml/min) high back pressure. Ensuring that 0.02 Pressure (psi) 0.03 Pressure (psi) 0.04 Pressure (psi) the appropriate tubing ID/flow rate combination is used will optimize the pumping system. *30 lengths of tubing used at corresponding flow rates for tubing pressure. Tubing was connected directly to the pump. All pre-filters were removed. Product #: (7010 valve with large-bore rotor seal) 0.018" make-before-break stator orifice 1 ml stainless steel " ID loop All other plumbing used is stainless steel " ID tubing (ml/min) Table 2: Rheodyne Factory Installed Large-bore Rotor Seal Valve Position and Pressure Standard 7010 Bore Size 7010 with Large-bore Rotor Load Position Pressure (psi) Inject Position Pressure (psi) Gilson Installed Load Position Pressure (psi) Gilson Installed Inject Position Pressure (psi) Solvent Miscibility and Polarity Xylene Water Tichloroethylene Toluene Tetrahydrofuran Di-Iso-Propyl Ether Iso-Propanol 6 n-propanol Pentane Solvent Miscibility Table Methyl Ethyl Ketone 5 Methyl-t-Butyl Ether 4 Methanol Hexane Heptane Di-Ethyl Ether Ethyl Acetate Ethanol Dioxane Dimethyl Sulfoxide 3 Dimethylformamide Dichloromethane 2 1,2-Dichloroethane 1 Cyclohexane Chloroform Carbon Tetrachloride Butyl Acetate n-butanol Benzene Acetonitrile Acetone Acetic Acid Solvent Polarity Index Refraction C UV(nm) Boiling Point ( C) Viscosity (cpoise) Solubility in water (%w/w) Acetic Acid Acetone Acetonitrile Benzene n-butanol Butyl Acetate Carbon Tetrachloride Chloroform Cyclohexane ,2-Dichloroethane Dichloromethane Dimethylformamide Dimethyl Sulfoxide Dioxane Ethanol Ethyl Acetate Di-Ethyl Ether Heptane Hexane Methanol Methyl-t-Butyl Ether Methyl Ethyl Ketone Pentane n-propanol Iso-Propanol Di-Iso-Propyl Ether Tetrahydrofuran Toluene Tichloroethylene Water Xylene Immiscible Miscible Immiscible means that in some proportions two phases will be produced. Synonym Table 1 Ethylene Chloride 2 Methylene Chloride 3 Methyl Sulfoxide 4 tert-butyl Methyl Ether 5 2-Butanone 6 2-Propanol Separation Scale-up Equations Scale-up Factor (diameter preparative) 2 scale-up factor = (diameter analytical) 2 flow rate preparative = scale-up factor analytical flow rate Gradient Duration (GD) length preparative (diameter preparative) 2 flow rate analytical GD preparative = GD analytical x x x length analytical (diameter analytical) 2 flow rate preparative Relative Polarity Compound Formula Solvent Polarity Chart Group Representative Solvent Compounds Non-polar R - H Alkanes Petroleum Ethers, Ligroin, Hexanes Ar - H Aromatics Toluene, Benzene R - O - R Ethers Diethyl Ether R - X Alkyl Halides Tetrachloromethane, Chloroform R - COOR Esters Ethyl Acetate R - CO - R Aldehydes and Ketones Acetone, Methyl Ethyl, Ketone R - NH 2 Amines Pyridine, Triethylamine R - OH Alcohols Methanol, Ethanol, Isopropanol, Butanol R - COHN 2 Amides Dimethylformamide R - COOH Carboxylic Acids Ethanoic Acid Polar H - OH Water Water Increasing Polarity Visit or call for more details on Gilson Instruments and Software. 63

34 Reference Common Conversion Tables Temperature Conversion Fractional inch-to-decimal-to-millimeter Conversion Tubing Internal Diameters & Volumes Pressure Conversion Celsius ( C) Fahrenheit ( F) Fraction (") Decimal Millimeter 1/ / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / Inch Millimeter Micron µl/in µl/mm Temperature Conversion Formulas C = F C = K F = 1.8 x C + 32 F = R K = C R = F C = degrees Celsius F = degrees Fahrenheit K = degrees Kelvin R = degrees Rankine kpa psi bar MPa Metric Prefixes Prefix Symbol Scientific Notation yotta Y zetta Z exa E peta P tera T giga G 10 9 mega M 10 6 kilo k 10 3 hecto h 10 2 deka da 10 deci d 10-1 centi c 10-2 milli m 10-3 micro µ 10-6 nano n 10-9 pico p femto f atto a zepto z yocto y Visit or call for more details on Gilson Instruments and Software. 65

35 Reference Gilson Applications Liquid Chromatography Liquid Chromatography continued clc System: An Automated Solution for Normal and Reversedphase Preparative HPLC with Analytical Purification Determination The clc system offers researchers the capabilities of both RP and NP chromatography on a single system, including data representing the throughput and analysis capabilities of the system. Optimizing Purified Sample Recovery on Reversed-phase HPLC Systems This application presents the factors that can directly affect purity and recovery when processing compounds on a preparative level. The following system parameters were evaluated: collection valve switch time, detector time constant delay, DMSO sandwich solvent, delay volume from detector to collection valve and software response. A Totally Integrated 2D System Incorporating Ion-exchange and RP-HPLC for Natural and Biological Products An automated two-dimensional application that incorporates ion-exchange and reversed-phase chromatography for the separation of complex mixtures. Data is presented that shows the usefulness of two dimensional chromatography and implementing alternative chromatography modes. Preparative HPLC: Factors and Parameters that Directly Affect Recovery of Collected Fractions Parameters discussed are column performance, mobile phase constituents, detector settings and collection parameters such as collection via level versus slope and delay volumes. The data offers guidelines for all chromatographers purifying compounds by preparative HPLC and can be implemented into existing systems. Separation of Flavonoids Through Automated Multiple Column Injections and Sample Concentration via C18 Column Capture Identical columns are configured in parallel for multiple injections sequenced by the sample peak elution. Sample is concentrated on a separate C18 preparative column, the purified compound is eluted in high organic solvent for ease of dry-down. Enhanced Recovery of Compound Mixtures by Concurrent Dissection and Concentration of Isolates A crude separation of a complex mixture into impure isolates. The impure isolates are reintroduced to the column. Front and back sections are cleaved to fractionate uncontaminated compound on separate columns for concentration. Final elution dispenses pure compound in high organic solvent for ease of dry-down. Automated Post-collection Concentration for Purified Preparative Fractions via SPE The method (as opposed to analytical applications involving SPE) works by collecting and then diluting the fraction(s), passing the fraction(s) through an SPE and ultimately eluting the concentrated fraction in a small amount of 100% organic solvent. Determination of within an Analytical System via Repeat Injections The data presented in this application shows that the Gilson HPLC system provides consistent and reproducible chromatography. A mixture of three compounds was injected repeatedly under analytical reversed-phase conditions. Novel Approach for Screening of Drug Absorption via an Automated System The system presented provides a faster alternative to other screening methods, as well as an inexpensive analysis estimate of drug permeability for hundreds of drug candidates in a fraction of the time of conventional screening methods. A Multi-purpose Preparative System for the Detection and Collection of Active and Non-active UV/VIS Compounds The process relies on an analytical scout analysis to determine the best gradient conditions to optimize the separation of the compounds under preparative conditions and minimize the number of fractions collected per run by incorporating ELS detection in addition to UV/VIS detection. Systematic Look at the Affects of Various Solvents, Injection Techniques and Sample Amounts in Preparative HPLC Columns Data is presented documenting the affects elicited in preparative chromatography under various conditions. Optimized Sample Load Capacity and Separation Through a Series of Short Preparative Columns By placing the smaller preparative columns in series, loading capacity and separations of compounds can increase with short run times and semi-preparative flow rates, minimizing the overall cost of the larger preparative columns and channeling within the column. Train Keep a Rolling and Isolation Gradients : A Method for Purifying a Large Quantity of Sample via Continuous Injection and Simultaneous Fraction Collection on Multiple FC Beds Train injection employs continuous injection of sample under isocratic conditions and continuous fraction collection. In addition, isolation gradients also offer an efficient purification of preparative samples when the train injection technique is not applicable, as is the case with complicated chromatograms. Optimizing Chiral Separations via an Automated Parallel Scouting System for On-line Preparative Chiral Purification The system is capable of screening chiral compounds on four separate columns in parallel, increasing the stereoselective screening by four fold. Technical Data for the Quad-Z 215 (fixed probes) Dispensing Small Volumes of DMSO This study determined the accuracy and CVs for the Quad-Z 215 (fixed probes) for low volume dispensing of DMSO. The results of the testing show the Quad-Z 215 is capable of transferring low volumes of DMSO from a 96-well format into an empty, flat-bottomed 96-well microplate. Quad-Z 215 with Disposable Tips: Implementing the Use of ZipTips for the Purification of Biological Samples This application demonstrates how the Quad-Z 215 automates sample clean-up using ZipTips. The Quad-Z 215 is also capable of completing numerous liquid handling techniques with or without tips under varying microplate formats. Reformatting Procedures Employing a Quad-Z 215 with Disposable Tips The volumetric accuracy and precision data presented show that the Quad-Z 215 with disposable tips offers researchers the consistency they require in all assays. The use of common tubes and microplates, along with the option of designing a custom rack, provides a high degree of versatility to accommodate variations in procedures. Automated Liquid Handling Accessing the E-Gel 96 System via the Quad-Z 215 with Disposable Tips This application examines the suitability of the Quad-Z 215 with disposable tips for the delivery of proteins to the wells of the E-Gel 96 gels for electrophoresis. A Compact Automated Liquid Handler for Microbatch Protein Crystallography A system that incorporates fixed and disposable tips on the Quad-Z 215. Independent probe motion and the use of virtual pumps make the system very versatile in the field of protein crystallography via microbatch techniques. Visit or call for more details on Gilson Instruments and Software. 67

36 Reference Gilson Applications continued An Automated SPE Procedure and Analysis for the Extraction of Chlorpromazine and Thioridazine from Plasma This application describes an automated SPE method coupled with HPLC analysis to determine the levels of Chlorpromazine and Thioridazine in human plasma. An Automated SPE Procedure and Analysis for the Determination of Ibuprofen and Ketoprofen in Plasma This application describes an automated SPE method coupled with HPLC analysis to determine the various levels of Ibuprofen and Ketoprofen in human plasma. The Ephedra Story: An Automated SPE Procedure and Analysis for the Determination of Ephedra and Ephedra-like Compounds in Dietary Supplements This application presents the advantages of using an automated SPE method coupled with HPLC, versus manual SPE, to determine the various levels of Ephedra and Ephedralike compounds in dietary supplements. Optimization of SPE by an Automated Method Development Procedure The system allows for the optimization of SPE process via multiple collection sites and various conditions without manual intervention. Incorporation of a trace enrichment cartridge to concentrate the eluent versus a dry-down step offers advantages to the dry-down bottle neck and analyte degradation. Solid Phase Extraction Automated Post-collection Concentration for Purified Preparative Fractions via SPE The method (as opposed to analytical applications involving SPE) works by collecting and then diluting the fraction(s), passing the fraction(s) through an SPE and ultimately eluting the concentrated fraction in a small amount of 100% organic solvent. Isolation and Determination of Capsaicin and Dihydrocapsaicin from Complex Matrices via Automated SPE and UV/ELS/MS Detection Matrices tested include dried habanero, topical analgesic cream and pepper spray. The system allows for the extraction procedure to be automatically optimized for each matrix without operator intervention, providing high recovery. Flash: Eliminating the Concentration/Dry-down Required for a Purified Compound by Preparative Chromatography Employing an activated media packed in an SPE/Flash cartridge (60 ml/10 gm), the automated system can intelligently identify the purified fractions and compound mass g, without time-consuming dry-down. Totally Automated Method for the Determination of Sudan Dyes in Food via On-line Filtration, SPE and HPLC Analysis The system presented offers a totally automated approach to the extraction of Sudan dyes from food products. The product is solubilized, filtered and SPE is performed prior to HPLC analysis, at detection limits below the EU of mg/kg. Quality Policy It is Gilson, Inc. s policy to promote a total commitment to quality by each individual throughout the Gilson organization worldwide. It is Gilson s goal that this commitment to quality will result in complete customer satisfaction with both Gilson s products and services. ISO 9001 Certification Gilson is committed to quality, and it shows in everything Gilson does from design to manufacturing, technical support and training. Due to this commitment to quality and customer satisfaction, Gilson has consistently been awarded with ISO 9001 certification. Gilson, Inc. and Gilson S.A.S. are both ISO 9001 certified. Training Gilson provides an extensive program of instrument and software training for users. Standard courses as well as sessions customized to the user s specific needs are offered at Gilson s innovative training center or in the user s own laboratory. Computer assisted training courses are also available on CD-ROM or the Gilson on-line education center, Gilson, Inc. World Headquarters 3000 Parmenter Street P.O. Box Middleton, WI , USA Tel: or Fax: sales@gilson.com service@gilson.com training@gilson.com hr@gilson.com oem@gilson.com A Rapid Extraction and Clean-up Procedure for the Determination of Amnesic Shellfish Poison Toxins in Shellfish A rapid sample preparation procedure has been developed via SPE to determine the levels of domoic acid in shellfish. Data supporting this approach is presented along with the automated SPE procedure incorporating trace enrichment cartridges to increase sensitivity of the assay. Optimizing Parameters that Influence Recovery in SPE A totally automated approach to optimize every aspect of an SPE method without manual intervention on an SPE instrument via fractionation, improving recovery and reducing coefficient of variance. Gilson S.A.S. 19, avenue des Entrepreneurs BP 145, F VILLIERS LE BEL, FRANCE Tel: 33-(0) Fax: 33-(0)

37 Gilson, Inc. World Headquarters 3000 Parmenter Street P.O. Box Middleton, WI , USA Tel: or Fax: Gilson S.A.S. 19, avenue des Entrepreneurs BP 145, F VILLIERS LE BEL, FRANCE Gilson, Inc. LT subject to change without notice.