QuickSplit TM Flow Splitters
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1 Quick TM Flow ters Quick TM Flow ter Features: Fluid resistor technology eliminates tedious adjustments to capillary tubing for split ratio optimization ratios are stable and reproducible, and not affected by changes in viscosity or pressure Adjustable metering valve enables precise direct control over split ratios Easy to use interchangeable fluid resistors make it possible to achieve split ratios from 1:1 to 20,000:1 Rugged stainless steel construction allows high pressure operation Ultra low dead volume fluidic design Applications include LC/MS, pre/post-column flow splitting, and flow fractionation Multiport flow splitter diverts the inlet flow into 3 or 4 channels for applications that employ multiple detectors and/or a fraction collector Makeup-Flow ter for mass directed fraction collection 1
2 Quick TM Flow ters The ASI Quick Flow ter is very elegant in its simplicity. ratios are created by two or more fluid resistors that form a parallel flow path. Quick Flow ters are available with a fixed or adjustable split ratio. Interchangeable fluid resistors make it easy to change split ratios uickly, eliminating tedious adjustments to capillary tubing. The technology can be applied to all applications where a controlled, reproducible split ratio is reuired including LC/MS, flow fractionation, pre/post-column flow splitting mass directed fraction collection, and capillary chromatography. ASI Quick Flow ters come in flow rate ranges which make them compatible with micro, analytical, semi-preparative and preparative inlet flows. Table of Contents Quick Fixed Flow ters... 4 Post-Column Applications Binary Fixed Flow ters... 5 Analytical... 6 Semi-Preparative... 7 Preparative... 8 High-Preparative... 8 Performance Data... 9 Multiport Fixed Flow ters Analytical Semi-Preparative Pre-Column Applications Binary Fixed Flow ters Analytical Fixed FS/Makeup-Flow ter Accessories Quick Makeup-Flow ter Manifolds Quick Adjustable Flow ters Post-Column Applications Analytical Semi-Preparative Preparative Selection Charts Pre-Column Applications Analytical Adjustable Flow ter Accessories
3 Quick TM Flow ters Post-Column Application - Applies to single and multiple Detectors While the diagram below does not cover all possible Post-Column Flow ter configurations, it depicts the most common application. Post-Column flow splitters specifications should be reviewed carefully in the subseuent product literature before selecting a splitter to order. If you have uestions about which splitter is right for your application, please contact the technical support group at ASI. Injector Add Quick TM Post-Column Detector A HPLC Column here B Mobile Phase HPLC Pump Pre-Column Application - Applies to single and multiple Columns While the diagram below does not cover all possible Pre-Column Flow ter configurations, it depicts the most common application. Pre-Column flow splitters specifications should be reviewed carefully in the subseuent product literature before selecting a splitter to order. If you have uestions about which splitter is right for your application, please contact the technical support group at ASI. Add Quick TM Pre-Column Detector A here HPLC Column A B Mobile Phase HPLC Pump Waste Injector B
4 Quick TM Fixed Flow ter Unlike conventional splitters that use long lengths of capillary tubing, the ASI Quick Fixed Flow ter uses two compact fluid resistor elements which are designed as cartridges for easy replacement. ASI fluid resistors are analogous to resistors used in an electrical circuit. Resistance values (L) are rated in PSI/mL/min. Because of the extremely low internal volume of the fluid resistors, the solvent composition in both resistors at any instant in time is the same, and therefore viscosity changes associated with gradient runs do not impact the split ratio. Quick Fixed Flow ters provide a fixed split ratio with extremely low dead volume. Delay volume on the low flow rate side is as low as 100 nanoliters depending upon the resistor cartridge selected. The split ratio is not affected by changes in solvent viscosity or pressure, and is extremely stable and reproducible. The interchangeable fluid resistors are available in a wide range of values which make it possible to create split ratios from 1:1 to as high as 20,000:1. The flow path of the Quick Fixed Flow ter contains two fluid resistors that form a parallel flow path. Both low and high flow rate streams pass through fixed resistor cartridges. The ratio of these two resistors creates the split ratio. To understand how the Quick Fixed Flow ter works it helps to look at a diagram, figure 1, of the fluid resistors in relation to the flow paths and how a split ratio is calculated. Figure 1. Schematic flow diagram of the Quick Fixed Flow ter Input Flow - Q Resistor - L2 Low Flow Rate - Q2 Resistor - L1 High Flow Rate - Q1 L1 L2 R = Fixed fluid resistor (resistance value varies depending on cartridge rating) = Fixed fluid resistor (resistance value varies depending on cartridge rating) = ratio = Q1/Q2 = Resistance ratio = L2/L1 Since the flow rate is indirectly proportional to resistance, changing the resistance in either flow path results in a change to the split ratio. Changing resistance is accomplished by exchanging the fixed fluid resistor cartridges with a resistor set that has different resistor ratings. The Quick Fixed Flow ter is shipped with resistors installed that deliver the nominal stated split ratio. The split ratios have a tolerance range of +/- 10 %. The exact split ratio is measured at ASI and is stated on the certificate shipped with the splitter. The input flow rate can be adjusted to compensate for the tolerance in split ratios. For instance, a 10 % increase in input flow rate will result in a 10 % increase in flow at both the low and high flow channels. Flow rate and viscosity changes will change the backpressure generated by the splitter, but will not affect the actual split ratio. The Quick Fixed Flow ter is shipped configured for either post-column or pre-column applications. 4
5 Post-Column Applications Post-column splitting is fairly straight forward. Devices contribute to chromatographic dispersion so care must be given to connecting tubing and fittings, especially at low flow rates. The pressure drop specification for all input flow ranges is 500 PSI with water at the corresponding inlet flow rate respectively. To assure a maximum pressure drop of 500 PSI across the splitter, please specify the inlet flow when the splitter is ordered. ters are shipped complete with fluid resistors installed. Quick TM Binary Fixed Flow ter This easy to use plug and play device comes with a predefined split ratio eliminating tedious adjustments to capillary tubing. ratio changes are accomplished by changing the resistor set. The split ratio is determined by the ratio of fluid resistors installed in the splitter manifold. The pressure drop across a fixed splitter for post column applications is typically low, less than 500 PSI. The low internal dead volume prevents excessive dispersion and the replaceable inlet filter insures robust operation. Available in models for analytical, semi-preparative, preparative and high-preparative inlet flow rates. Interchangeable Fluid Resistor Cartridge & Ratios ratios and resistor cartridges other than those listed can be ordered to custom configure the Quick Fixed Flow ter. Please contact ASI technical support for additional information about custom splitters. We will gladly assist you in determining the best splitter configuration for your application. 5
6 Post-Column Applications continued Quick TM Binary Fixed Flow ters Analytical ters - Binary Analytical range, 0.1 ml/min. to 5 ml/min. input flow These splitters will produce under 500 PSI backpressure with water at 1.0 ml/min.* The backpressure will decrease or increase in proportion to flow rate changes. In order to assure <500 PSI pressure drop across the splitter, please specify the inlet flow when you order. Does not come with mounting bracket, please see page 14 for mounting bracket order information. A nalytical Fixed Flow ter, Post-Column 2,000: PO10-03 A nalytical Fixed Flow ter, Post-Column 1,000: PO10-04 A nalytical Fixed Flow ter, Post-Column 500: PO10-05 A nalytical Fixed Flow ter, Post-Column 200: PO10-06 A nalytical Fixed Flow ter, Post-Column 100: PO10-07 A nalytical Fixed Flow ter, Post-Column 50: PO10-08 A nalytical Fixed Flow ter, Post-Column 20: PO10-09 A nalytical Fixed Flow ter, Post-Column 10: PO10-10 A nalytical Fixed Flow ter, Post-Column 5: PO10-11 A nalytical Fixed Flow ter, Post-Column 3: PO10-12 A nalytical Fixed Flow ter, Post-Column 1: PO10-13 A nalytical Fixed Flow ter, Post-Column 620-PO10-CS Analytical Replacement Resistor Sets - Binary A nalytical Fixed Flow ter Resistor Set, Post-Column 2,000: A nalytical Fixed Flow ter Resistor Set, Post-Column 1,000: A nalytical Fixed Flow ter Resistor Set, Post-Column 500: A nalytical Fixed Flow ter Resistor Set, Post-Column 200: A nalytical Fixed Flow ter Resistor Set, Post-Column 100: A nalytical Fixed Flow ter Resistor Set, Post-Column 50: A nalytical Fixed Flow ter Resistor Set, Post-Column 20: A nalytical Fixed Flow ter Resistor Set, Post-Column 10: A nalytical Fixed Flow ter Resistor Set, Post-Column 5: A nalytical Fixed Flow ter Resistor Set, Post-Column 3: A nalytical Fixed Flow ter Resistor Set, Post-Column 1: A nalytical Fixed Flow ter Resistor Set, Post-Column CS 6
7 Post-Column Applications continued Quick TM Binary Fixed Flow ters Semi-Preparative ters - Binary Semi-Prep range, 5 ml/min. to 40 ml/min. input flow These splitters will produce under 500 PSI backpressure with water at 20.0 ml/min.** The backpressure will decrease or increase in proportion to flow rate changes. In order to assure <500 PSI pressure drop across the splitter, please specify the inlet flow when you order. Does not come with mounting bracket. Mounting bracket is not available. S emi-preparative Fixed Flow ter, Post-Column 20,000: PO20-00 S emi-preparative Fixed Flow ter, Post-Column 10,000: PO20-01 S emi-preparative Fixed Flow ter, Post-Column 5,000: PO20-02 S emi-preparative Fixed Flow ter, Post-Column 2,000: PO20-03 S emi-preparative Fixed Flow ter, Post-Column 1,000: PO20-04 S emi-preparative Fixed Flow ter, Post-Column 500: PO20-05 S emi-preparative Fixed Flow ter, Post-Column 620-PO20-CS Semi-Preparative Replacement Resistor Cartridges - Binary Semi-Preparative Fixed Flow ter High Flow Resistor Cartridge, Post-Column Semi-Preparative Fixed Flow ter High Flow Resistor Cartridge, Post-Column Semi-Preparative Fixed Flow ter High Flow Resistor Cartridge, Post-Column Semi-Preparative Fixed Flow ter High Flow Resistor Cartridge, Post-Column Semi-Preparative Fixed Flow ter High Flow Resistor Cartridge, Post-Column Semi-Preparative Fixed Flow ter High Flow Resistor Cartridge, Post-Column Semi-Preparative Fixed Flow ter High Flow Resistor Cartridge, Post-Column 20,000: H 10,000: H 5,000: H 2,000: H 1,000: H 500: H CSH * ** Flow rates higher than 1.0 ml/min. can be used, but may result in a higher pressure drop across the splitter. Flow rates higher than 20.0 ml/min. can be used, but may result in a higher pressure drop across the splitter. 7
8 Post-Column Applications continued Quick TM Binary Fixed Flow ters Preparative ters - Binary Prep range, 30 ml/min. to 200 ml/min. input flow These splitters will produce under 500 PSI backpressure with water at the specified inlet flow rate.* The backpressure will decrease or increase in proportion to flow rate changes. In order to assure <500 PSI pressure drop across the splitter, please contact ASI for assistance in configuring your Fixed ter prior to ordering. Preparative Fixed Flow ter, Post-Column Input Flow Range: ml/min. 620-PO40-CS Does not come with mounting bracket. Mounting bracket is not available. Preparative Replacement Resistor Cartridge/Resistor Set - Binary Preparative Fixed Flow ter, Post-Column, High Flow Resistor Cartridge Input Flow Range: ml/min. High-Preparative ters - Binary High-Prep range, 200 ml/min. to 1,000 ml/min. input flow These splitters will produce under 500 PSI backpressure with water at the specified inlet flow rate.* The backpressure will decrease or increase in proportion to flow rate changes. In order to assure <500 PSI pressure drop across the splitter, please contact ASI for assistance in configuring your Fixed ter prior to ordering CSH High-Preparative Fixed Flow ter, Post-Column Input Flow Range: 200-1,000 ml/min. 620-PO60-CS Does not come with mounting bracket, please see page 14 for mounting bracket order information. High-Preparative Replacement Resistor Cartridge/Resistor Set - Binary High-Preparative Fixed Resistor Set Input Flow Range: 200 Flow ter, Post-Column, - 1,000 ml/min CS * Flow rates higher than the specified inlet flow rate can be used, but may result in a higher pressure drop across the splitter. 8
9 Effect of Gradient on Flow Rate Stability ACN Gradient 5-95% in 20 min Flow Rate ul/min µl/min PSI Conditions: ter 620-PO10-CS Ratio 15:1 Inlet Flow 400µL/min % ACN LF SR PSI 0 Effect of Viscosity on Ratio 30 Ratio vs %IPA Ratio Conditions: Ratio Measurements ter 620-PO10-CS Ratio 15:1 90%H20/10%IPA 50%H20/50%IPA 10%H20/90%IPA Inlet Flow 400µL/min. 9
10 Post-Column Applications continued Post-column splitting is fairly straight forward. Devices contribute to chromatographic dispersion so care must be given to connecting tubing and fittings, especially at low flow rates. The pressure drop specification for both analytical and semi-preparative fixed splitters is 500 PSI with water at a flow rate of 1.0 ml/min. and 20.0 ml/min. respectively. To assure a maximum pressure drop of 500 PSI across the splitter, please specify the inlet flow when the splitter is ordered. ters are shipped complete with fluid resistors installed. Quick TM Multiport Fixed Flow ters Divides the incoming flow stream into 3 or 4 channels. The Quick Multiport Flow ter is ideal for applications that use multiple detectors and/or a fraction collector. ratios can be configured to be identical in each channel or custom configured to accommodate specific flow rates at each channel. The low internal dead volume prevents excessive dispersion and the replaceable inlet filter insures robust operation. Available in models for analytical and semi-preparative inlet flow rates. Please contact ASI for assistance in configuring your Quick Multiport Fixed ter prior to ordering. Please specify inlet flow rate and desired output flow rate in each channel when you order. Quick Multiport Fixed Flow ter 10
11 Post-Column Applications continued Quick TM Multiport Fixed Flow ters Please contact ASI for assistance in configuring your Quick Multiport Fixed ter prior to ordering. Please specify inlet flow rate and desired output flow rate in each channel when you order. Analytical ters - Multiport Analytical range, 0.1 ml/min. to 5 ml/min. input flow These splitters will produce under 500 PSI backpressure with water at 1.0 ml/min.* The backpressure will decrease or increase in proportion to flow rate changes. A nalytical Three Port Fixed Flow ter, Post-Column A nalytical Four Port Fixed Flow ter, Post-Column Comes with mounting bracket. 630-PO10-CS 640-PO10-CS Semi-Preparative ters - Multiport Semi-Prep range, 5 ml/min. to 40 ml/min. input flow These splitters will produce under 500 PSI backpressure with water at 20.0 ml/min.** The backpressure will decrease or increase in proportion to flow rate changes. Semi-Preparative Post-Column Semi-Preparative Post-Column Three Port Fixed Flow ter, Four Port Fixed Flow ter, 630-PO20-CS 640-PO20-CS Comes with mounting bracket. * ** Flow rates higher than 1.0 ml/min. can be used, but may result in a higher pressure drop across the splitter. Flow rates higher than 20.0 ml/min. can be used, but may result in a higher pressure drop across the splitter. 11
12 Pre-Column Applications Pre-column splitting is used for micro, capillary, and nano HPLC applications, where the flow from the pump is split from analytical flow rates down to microliter or nanoliter flows. It is important to note that even though the split ratio created by the splitter valve will remain constant, the split ratio will change when a HPLC column is installed. This is due to the added resistance on the low flow rate channel from the HPLC column. This added resistance must be factored in to make sure the fluid resistors selected for the flow splitter provide the correct split ratio. Please contact ASI if you need assistance. ters are shipped complete with fluid resistors installed. When ordering a pre-column flow splitter please provide ASI with the column flow rate and back pressure. If the inlet flow rate or column pressure specification is not provided, ASI will configure pre-column flow splitters assuming a 0.5 ml/min. splitter inlet flow rate and a pressure drop across the column of 1,500 PSI. Adjustments to the low flow rate channel can be made by increasing or decreasing the splitter inlet flow rate.! Please consider Pre-column Adjustable Flow ters on page 23 when you need more than one split ratio. Quick TM Binary Fixed Flow ter This easy to use plug and play device comes with a predefined split ratio eliminating tedious adjustments to capillary tubing. ratio changes are accomplished by changing the resistor set. The split ratio is determined by the ratio of fluid resistors installed in the splitter manifold. The pressure drop across a fixed splitter for pre-column applications where the pressure is nominally 1,500 PSI. The low internal dead volume prevents excessive dispersion and the replaceable inlet filter insures robust operation. Available in models for analytical inlet flow rates. & Ratios Interchangeable Fluid Resistor Cartridge ratios and resistor cartridges other than those listed can be ordered from ASI to custom configure the Quick Fixed Flow ter. Please contact technical support for additional information about custom splitters. We will gladly assist you in determining the best splitter configuration for your application. 12
13 Pre-Column Applications continued Quick TM Binary Fixed Flow ters Analytical ters - Binary Analytical range, 0.1 ml/min. to 0.5 ml/min. input flow The pressure drop across a pre-column flow splitter will generally be between 1,000 PSI to 3,500 PSI. The exact pressure drop will depend on how the splitter is configured. Does not come with mounting bracket, please see page 14 for mounting bracket order information. A nalytical Fixed Flow ter, Pre-Column 2,000: PR10-03 A nalytical Fixed Flow ter, Pre-Column 1,000: PR10-04 A nalytical Fixed Flow ter, Pre-Column 500: PR10-05 A nalytical Fixed Flow ter, Pre-Column 200: PR10-06 A nalytical Fixed Flow ter, Pre-Column 100: PR10-07 A nalytical Fixed Flow ter, Pre-Column 50: PR10-08 A nalytical Fixed Flow ter, Pre-Column 20: PR10-09 A nalytical Fixed Flow ter, Pre-Column 10: PR10-10 A nalytical Fixed Flow ter, Pre-Column 620-PR10-CS Analytical Replacement Resistor Sets - Binary A nalytical Fixed Flow ter Resistor Set, Pre-Column 2,000: PR00-03 A nalytical Fixed Flow ter Resistor Set, Pre-Column 1,000: PR00-04 A nalytical Fixed Flow ter Resistor Set, Pre-Column 500: PR00-05 A nalytical Fixed Flow ter Resistor Set, Pre-Column 200: PR00-06 A nalytical Fixed Flow ter Resistor Set, Pre-Column 100: PR00-07 A nalytical Fixed Flow ter Resistor Set, Pre-Column 50: PR00-08 A nalytical Fixed Flow ter Resistor Set, Pre-Column 20: PR00-09 A nalytical Fixed Flow ter Resistor Set, Pre-Column 10: PR00-10 A nalytical Fixed Flow ter Resistor Set, Pre-Column 620-PR00-CS 13
14 Fixed FS / Makeup-Flow ter Accessories Replacement Inlet Filters Inlet Filter Assembly, 2 micron.063" dia. 5/Pack, 1 µl Volume Inlet Filter & Housing Assembly, 10 micron.125" dia. each, 4 µl Volume Inlet Filter & Housing Assembly, 10 micron.188" dia. each, 10 µl Volume Inlet Filter & Housing Assembly, 20 micron.188" dia. each, 12 µl Volume Inlet Filter & Housing Assembly, 20 micron.188" dia. each, 12 µl Volume Straight Thru Hole, No Filter 5/Pack, 0.1 µl Volume Straight Thru Hole, No Filter 5/Pack, 1 µl Volume Mounting Bracket 620 Applications Fixed FS - Analytical Range Makeup-Flow ter, Inlet Port - for 620-PO90-01 Makeup-Flow ter, Makeup Inlet Port - All Ranges Fixed FS - Semi-Prep Range Makeup-Flow ter, Inlet Port - for 620-PO90-02, 04 Fixed FS - Prep Range Makeup-Flow ter, Inlet Port - for 620-PO90-03 Fixed FS - Prep Range Makeup-Flow ter, Inlet Port - for 620-PO Fixed FS - High-Prep Range Fixed FS - Analytical Range Fixed FS - High-Prep Range Mounting Bracket for 620-PO10, 620-PO60 and 620-PR Capillary Resistor Capillary Resistor Flow Measurement Kits Flow Rate Range 50 nl/min. to Flow Rate Range 5 µl/min. to Flow Rate Range 10 µl/min. to Flow Rate Range 25 µl/min. to 5 µl/min. 25 µl/min. 100 µl/min. 500 µl/min. interfaces tubing to 360 µm OD fused silica interfaces to 1/16 OD PEEK tubing interfaces to 1/16 OD PEEK tubing interfaces to 1/16 OD PEEK tubing 620-PR00-CP S S S S 14
15 Quick TM Makeup-Flow ter Manifolds The ASI Quick Makeup-Flow ter Manifold is designed for post-column applications where a small amount of flow from an HPLC column is efficiently combined with a makeup-flow before it reaches the detector. Although there are many variations of this type of application, one of the most common involves splitting a small portion of the outlet flow from a preparative HPLC column which is then combined and diluted with a makeup-flow. The combined makeup-flow is used by a detector, typically Mass Spectrometry or UV, to trigger fraction collection from the remaining flow. A minimum delay time of 5 seconds (maximum inlet flow) is built into the splitter to insure proper seuencing between detection and fraction collection. Uniue integral resistor design eliminates tees and fittings within the splitter, resulting in extremely low peak dispersion. An additional binary splitter (see page 5) can be added after the manifold to allow splitting the makeup-flow stream prior to entering the detector. configuration orders must include inlet flow, split ratio, and delay time. In order to assure <500 PSI pressure drop across the splitter, please specify the inlet flow when you order. Inlet from Preparative HPLC Column Makeup Inlet R2/R1? R2 R1 12 $$ $ Delay Coil High Flow Resistor Low Flow Resistor High Flow Outlet to Fraction Collector Low Flow Outlet to Detector(s) $ $ ASI Binary ter (Optional) Quick Makeup-Flow ter Manifold Common Hydraulic Schematic for Makeup-Flow ter - Applies to single and multiple Detectors While the diagram on page 16 does not cover all possible Makeup-Flow ter configurations, it depicts the most common application. Post column flow splitters specifications should be reviewed carefully in the subseuent product literature before selecting a splitter to order. If you have uestions about which splitter is right for your application, please contact the technical support group at ASI. 15
16 Quick TM Makeup-Flow ter Manifolds Common Application Diagram Mobile Phase Prep. HPLC Pump Injector HPLC Column Prep. Inlet Add Quick TM Makeup-Flow ter here Prep. Delay Coil Fraction Collector Outlet Makeup-Flow Inlet Makeup-Flow Mass Spec Outlet Detector A Mobile Phase Makeup-Flow HPLC Pump ASI Post-Column Flow ter (optional) B Quick TM Makeup-Flow ter Manifolds M akeup-flow ter Manifold F low Range: 1-20 ml/min. 1,000: PO90-01 M akeup-flow ter Manifold F low Range: ml/min. 1,000: PO90-02 M akeup-flow ter Manifold F low Range: ml/min. 5,000: PO90-03 M akeup-flow ter Manifold F low Range: ml/min. 10,000: PO90-04 Makeup-Flow ter Manifold F low Range: 620-PO90-CS Please see Page 14 for Replacement Parts 16
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