Fixed Flow Splitter *
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1 Fixed Flow * *Patent Pending Analytical Scientific Instruments Fluid resistor technology eliminates tedious adjustments to capillary tubing to change split ratios. Easy to use interchangeable fluid resistors create a wide range of split ratios. Split ratios are stable and reproducible, and not affected by changes in viscosity or pressure. Multi port flow splitter splits one input flow into 3 to 8 channels: making it ideal for applications that employ multiple detectors and/or a fraction collector. ugged stainless steel construction allows ultra-high pressure limits (up to 0,000 PSI). ASI splitter technology can be applied to all applications where a controlled, reproducible split ratio is required including LC/MS, flow fractionation, pre/post column flow splitting, and capillary chromatography. Create split ratios from : to 20,000:. Ultra-low dead volume fluidic design. Delay volume on the capillary side as low as 00 nanoliters. eplaceable inlet filter prevents clogging. ASI Fixed Flow s provide a fixed split ratio with extremely low dead volume. Delay volume on the low flow rate side is as low as 00 nanoliters, depending upon the resistor cartridge selected. The flow split ratio is not affected by changes in solvent viscosity or pressure, and is extremely stable and reproducible. Unlike conventional splitters that use long lengths of capillary tubing, the Fixed Flow uses compact fluid resistor elements which are designed as cartridges for easy replacement. Because of the extremely low internal volume of the fluid resistors, the solvent composition in both resistors at any instant in time is the same, and therefore viscosity changes associated with gradient runs do not impact the split ratio. The interchangeable fluid resistors are available in a wide range of values that make it possible to create split ratios from : to as high as 20,000:. To understand how the Flow works it helps to look at a diagram of the fluid resistors in relation to the flow paths and how a split ratio calculated. Input Flow - Q esistor - L esistor - L2 Low Flow ate - Q2 High Flow ate - Q L L2 = Split ratio = Q/Q2
2 Since the flow rate is indirectly proportional to resistance, changing the resistance in either flow path results in a change to the split ratio. Changing resistance is accomplished by exchanging the fixed fluid resistor cartridges with a resistor set that has a different resistance rating. The Fixed Flow is shipped with resistors installed that deliver the nominal stated split ratio. The split ratios have a tolerance range of +/- 0%. The exact split ratio is measured at ASI and is stated on the certificate shipped with the splitter. The input flow rate can be adjusted to compensate for the tolerance in split ratios. For instance, a 0% increase in input flow rate will result in a 0% increase in flow at both the capillary and waste stream channels. Flow rate and viscosity changes will change the backpressure generated by the splitter, but will not affect the actual split ratio. Binary Fixed Flow Series 620 Interchangeable fluid resistors eliminate the need to cut capillary tubing to change split ratio. ugged stainless steel construction allows ultra-high pressure limits (up to 0,000PSI). Split ratios are not affected by changes in viscosity or pressure. Split ratios are set at the factory but can be changed by replacing the resistor set. Split ratios from : to 20,000:. Internal dead volume less than as low as 00nL on the low flow rate side. eplaceable inlet filter prevents plugging. Multiport Fixed Flow Series Splits incoming flow stream into 3 to 8 channels. The Multiport Flow is ideal for applications that use multiple detectors and/or a fraction collector. Split ratios can be configured to be identical in each channel or configured to accommodate specific flow rates at each channel. Split ratios at each channel can be changed at any time by simply replacing the fluid resistor. Low internal dead volume prevents excessive dispersion. eplaceable inlet filter prevents clogging. Binary Fixed Flow Series 620 Multiport Fixed Flow Series
3 Selecting the Optimum Fixed Flow Pre-column Flow Splitting Pre-column splitting is used for micro, capillary, and nano HPLC applications, where the flow from the pump is split from analytical flow rates down to microliter or nanoliter flows. It is important to note that even though the split ratio created by the splitter valve will remain constant, the split ratio will change when a capillary column is installed. This is due to the added resistance on the low flow rate channel from the column. This added resistance must be factored in when the split ratio is calculated and before a splitter valve is selected. Please contact ASI if you need assistance. Also, if the delay volume of the capillary column is large enough to allow a significant solvent gradient to exist in the column, then the viscosity in the column will not be the same as in the resistor inside the splitter, and this may begin to affect the split ratio. Of course, this will only be a factor when running a gradient with solvents of different viscosities. Both of these effects on split ratio are repeatable, and will not affect the retention time reproducibility. However, these effects can be reduced by maximizing the resistor value on the low flow rate channel. Post-column Flow Splitting Post-column splitting is fairly straight forward. Like pre-column flow splitting, any significant additional pressure (resistance) down stream from the splitter may affect the split ratio. Post-column devices also contribute to chromatographic dispersion so care must given to connecting tubing and fittings, especially at low flow rates.
4 Calculate Split atio The split ratio can also be determined from the input flow rate Q and the capillary output flow rate Q2 that is required: = (Q- Q2) /Q2 Q Q 2 : Split ratio : Input flow rate : Low flow rate channel Input Flow - Q esistor - L esistor - L2 Low Flow ate - Q2 High Flow ate - Q L L2 = Split ratio = Q/Q2 Note: To calculate flow rate through the low flow rate channel, back pressure and resistor value, use the following relation Q2 = ( P/)V Where Q2 is the output flow rate through the low flow rate channel, ml/min. P V = back pressure generated by splitter valve, PSI = resistor value, as stated on label (PSI/mL/min.) = Viscosity, Centipoise Adjustable Flow For those users who require an exact split ratio and who cannot adjust the input flow rate, the ASI or Adjustable Flow is recommended. The ASI technical service department is available to make recommendations for your specific requirements. Please contact ASI at: , Configuring the Fixed Flow for Custom Split atio anges A variety of resistor cartridges can be ordered from ASI to configure the Adjustable Flow for split ratios other than those listed. Please contact technical service at for assistance in selecting the correct resistor cartridges. We will gladly assist you in determining the best splitter configuration for your application.
5 Ordering Information Binary Fixed Flow Manifold The Following Manifolds Come Complete with Fluid esistors Installed. Manifold, Prep range, 5 ml/min. to 40 ml/min. input flow These splitter manifolds will produce under 500 PSI backpressure with water at 20.0 ml/min. The backpressure will decrease in direct proportion to a decrease in flow rate. Flow rates higher than 40.0 ml/ Manifold, atio = 20,000: Manifold, atio = 0,000: Manifold, atio = 5,000: Manifold, Analytical range, 0. ml/min. to 0 ml/min. input flow These splitter manifolds will produce under 500 PSI backpressure with water at.0 ml/min. The backpressure will decrease in direct proportion to a decrease in flow rate. Flow rates higher than.0 ml/ Manifold, atio = 2,000: Manifold, atio =,000: Manifold, atio = 500: Manifold, atio = 200: Manifold, atio = 00: Manifold, atio = 50: Manifold, atio = 20: Manifold, atio = 0: Manifold, atio = 5: Manifold, atio = 3: Manifold, atio = :
6 Binary Fixed Flow esistor Set esistor Set for Manifold, atio = 20,000: esistor Set for Manifold, atio = 0,000: esistor Set for Manifold, atio = 5,000: esistor Set for Manifold, atio = 2,000: esistor Set for Manifold, atio =,000: esistor Set for Manifold, atio = 500: esistor Set for Manifold, atio = 200: esistor Set for Manifold, atio = 00: esistor Set for Manifold, atio = 50: esistor Set for Manifold, atio = 20: esistor Set for Manifold, atio = 0: esistor Set for Manifold, atio = 5: esistor Set for Manifold, atio = 3: esistor Set for Manifold, atio = : Binary Fixed Flow Inlet Filter Inlet Filter, 2 micron x.25 dia. pkg 5, 4µL volume Inlet Filter, 2 micron x.063 dia. pkg 5, µl volume standard for prep range $50.00 standard for analytical range $ Straight thru hole, No filter $50.00
7 Multiport Fixed Flow Manifold The Following Manifolds Come Complete with Fluid esistors Installed. Please call ASI for assistance in configuring your multi port splitter prior to ordering. Manifold, Prep range, 5 ml/min. to 40 ml/min. input flow These splitter manifolds will produce under 500 PSI backpressure with water at 40.0 ml/min. The backpressure will decrease in direct proportion to a decrease in flow rate. Flow rates higher than 40.0 ml/ Three Ports Manifold, atio = Four Ports Manifold, atio = Five Ports Manifold, atio = Six Ports Manifold, atio = Seven Ports Manifold, atio = Eight Ports Manifold, atio = $, $, $, $2,85.00 $2, $2, Manifold, Analytical range, 0. ml/min. to 0 ml/min. input flow These splitter manifolds will produce under 500 PSI backpressure with water at.0 ml/min. The backpressure will decrease in direct proportion to a decrease in flow rate. Flow rates higher than.0 ml/ Three Ports Manifold, atio = Four Ports Manifold, atio = Five Ports Manifold, atio = Six Ports Manifold, atio = Seven Ports Manifold, atio = Eight Ports Manifold, atio = $, $, $, $2,85.00 $2, $2, Multiport Fixed Flow esistor Set Please use the resistor values stated on the individual cartridges to reorder. ev. 4/8/02
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