HyperShear TM HPLC Mixers

Transcription

1 HyperShear TM HPLC s HyperShear TM HPLC Features: Static or Dynamic s with highly efficient vortex shear mixing Reduce baseline noise, increase sensitivity, and improve gradient accuracy Mixing volume optimization is easy with interchangeable mixer cartridges Available for In-line,, and formats in SS or PEEK Ideal for microbore HPLC and LC/MS Compact design is easily integrated into any HPLC system Increased reaction efficiency for post column derivatization Decreased mixing and delay volume without sacrificing mixing efficiency 15,000 PSI High Pressure SS Static s are available 42 Analytical Scientific Instruments, Inc.

2 HyperShear TM HPLC s ASI manufactures a wide range of s to solve the most demanding HPLC mixing problems. ASI HyperShear HPLC s incorporate a highly efficient cross-flow shearing mechanism which produces vortex shear mixing over a wide flow range. This mixing technology typically delivers between 25 % to 200 % better mixing efficiency compared to conventional stir bar or tortuous path mixers. HyperShear HPLC s are available in both static and dynamic formats with volumes ranging from 0.5 µl to 1.5 ml. Most mixers are available in biocompatible PEEK. Comparison of Baseline Noise The baseline of this plot has been enlarged to show the noise levels associated with each mixer. Note the significant reduction in baseline noise with the ASI static mixer. Conditions: Gradient Program: CH3CN/H2O = 50/50 --> (15 min.) 90/10 Flow Rate: 0.1mL/min. Temp.: 40 C Sample Size: 1µL Sample: Solvent front Detection Wavelength: 254nm Table of Contents HyperShear Static s Micro Flow Series, Mixing Volumes 0.5 µl to 25 µl Low Flow Series, Mixing Volumes 50 µl to 250 µl Analytical Flow Series, Mixing Volumes 350 µl and 500 µl High Flow Series, Mixing Volumes 800 µl to 1.5 ml Combo Micro Series, Mixing Volumes 1 µl to 100 µl Static Application Notes Comparison Data - LEE, Agilent & Shimadzu HyperShear Dynamic s

3 Static s Micro Flow Series Stainless Steel * Please add HP to end of part number for High Pressure application Complete Assembly - Cartridge and Housing Assembly SS 0.5 µl Assembly SS 1 µl Assembly SS 2 µl Assembly SS 5 µl Assembly SS 10 µl Assembly SS 25 µl Assemblies Port Size: CPI ( Standard HPLC Fittings for 1/16 Tube) 0.5µL, 1µL, 2µL, 5µL, 10µL or 25µL Cartridge FLOWLOCK INPUT / OUTPUT ONE EACH END 44 Analytical Scientific Instruments, Inc.

4 Static s Tee Complete Assembly - Cartridge and Housing Tee Assembly SS 0.5 µl Tee Assembly SS 1 µl Tee Assembly SS 2 µl Tee Assembly SS 5 µl Tee Assembly SS 10 µl Tee Assembly SS 25 µl Tee Complete Assembly - Cartridge and Housing ASI Part Number Tee Assembly SS 0.5 µl Tee Assembly SS 1 µl Tee Assembly SS 2 µl Tee Assembly SS 5 µl Tee Assembly SS 10 µl Tee Assembly SS 25 µl / Assemblies Port Size: CPI FLOWLOCK OUTPUT PORTS (TWO OR THREE) 0.5µL, 1µL, 2µL, 5µL, 10µL or 25µL Cartridge FLOWLOCK INPUT PORT Cartridges / Housings Cartridge SS 0.5 µl Cartridge SS 1 µl Cartridge SS 2 µl Cartridge SS 5 µl Cartridge SS 10 µl Cartridge SS 25 µl Micro Flow Series SS Housing Tee Micro Flow Series SS Housing Tee Micro Flow Series SS Housing

5 Static s Micro Flow Series Biocompatible PEEK Complete Assembly - Cartridge and Housing Assembly PEEK 0.5 µl Assembly PEEK 1 µl Assembly PEEK 2 µl Assembly PEEK 5 µl Assembly PEEK 10 µl Assembly PEEK 25 µl B B B B B B Assemblies Port Size: CPI ( Standard HPLC Fittings for 1/16 Tube) AS I FLOWLOCK INPUT / OUTPUT ONE EACH END 0.5µL, 1µL, 2µL, 5µL, 10µL or 25µL Cartridge 46 Analytical Scientific Instruments, Inc.

6 Static s Tee Complete Assembly - Cartridge and Housing Tee Assembly PEEK 0.5 µl Tee Assembly PEEK 1 µl Tee Assembly PEEK 2 µl Tee Assembly PEEK 5 µl Tee Assembly PEEK 10 µl Tee Assembly PEEK 25 µl B B B B B B Tee Complete Assembly - Cartridge and Housing Tee Assembly PEEK 0.5 µl Tee Assembly PEEK 1 µl Tee Assembly PEEK 2 µl Tee Assembly PEEK 5 µl Tee Assembly PEEK 10 µl Tee Assembly PEEK 25 µl ASI Part Number B B B B B B / Assemblies Port Size: CPI FLOWLOCK OUTPUT PORTS (TWO OR THREE) FLOWLOCK INPUT PORT AS I 0.5µL, 1µL, 2µL, 5µL, 10µL or 25µL Cartridge Cartridges / Housings Cartridge PEEK 0.5 µl Cartridge PEEK 1 µl Cartridge PEEK 2 µl Cartridge PEEK 5 µl Cartridge PEEK 10 µl Cartridge PEEK 25 µl Micro Flow Series PEEK Housing Tee Micro Flow Series PEEK Housing Tee Micro Flow Series PEEK Housing B B B B B B B B B 47

7 Static s Low Flow Series Stainless Steel * Please add HP to end of part number for High Pressure application Complete Assembly - Cartridge and Housing Assembly SS 50 µl Assembly SS 150 µl Assembly SS 250 µl Assemblies Port Size: CPI ( Standard HPLC Fittings for 1/16 Tube) 50µL, 150µL or 250µL Cartridge FLOWLOCK INPUT / OUTPUT ONE EACH END 48 Analytical Scientific Instruments, Inc.

8 Static s Tee Complete Assembly - Cartridge and Housing Tee Assembly SS 50 µl Tee Assembly SS 150 µl Tee Assembly SS 250 µl Tee Complete Assembly - Cartridge and Housing Tee Assembly SS 50 µl Tee Assembly SS 150 µl Tee Assembly SS 250 µl / Assemblies Port Size: CPI 50µL, 150µL or 250µL Cartridge FLOWLOCK INPUT PORT Cartridges / Housings FLOWLOCK OUTPUT PORTS (TWO OR THREE) Cartridge SS 50 µl Cartridge SS 150 µl Cartridge SS 250 µl Low Flow Series SS Housing Tee Low Flow Series SS Housing Tee Low Flow Series SS Housing

9 Static s Low Flow Series Biocompatible PEEK Complete Assembly - Cartridge and Housing Assembly PEEK 50 µl Assembly PEEK 150 µl Assembly PEEK 250 µl B B B Assemblies Port Size: CPI ( Standard HPLC Fittings for 1/16 Tube) 50µL, 150µL or 250µL Cartridge FLOWLOCK INPUT / OUTPUT ONE EACH END 50 Analytical Scientific Instruments, Inc.

10 Static s Tee Complete Assembly - Cartridge and Housing Tee Assembly PEEK 50 µl Tee Assembly PEEK 150 µl Tee Assembly PEEK 250 µl B B B Tee Complete Assembly - Cartridge and Housing Tee Assembly PEEK 50 µl Tee Assembly PEEK 150 µl Tee Assembly PEEK 250 µl B B B / Assemblies Port Size: CPI 50µL, 150µL or 250µL Cartridge ASI FLOWLOCK INPUT PORT FLOWLOCK OUTPUT PORTS (TWO OR THREE) Cartridges / Housings Cartridge PEEK 50 µl Cartridge PEEK 150 µl Cartridge PEEK 250 µl Low Flow Series PEEK Housing Tee Low Flow Series PEEK Housing Tee Low Flow Series PEEK Housing B B B B B B 51

11 Static s Analytical Flow Series Stainless Steel, Biocompatible PEEK * Please add HP to end of part number for High Pressure application Complete Assembly - Cartridge and Housing Stainless Steel Biocompatible PEEK - HP is not available Assembly SS 350 µl Assembly SS 500 µl Assembly PEEK 350 µl Assembly PEEK 500 µl B B Assemblies Port Size: CPI ( Standard HPLC Fittings for 1/16 Tube) 350µL or 500µL Cartridge FLOWLOCK INPUT / OUTPUT ONE EACH END 52 Analytical Scientific Instruments, Inc.

12 Complete Assembly, Stainless Steel - Cartridge and Housing Tee Assembly SS 350 µl Tee Assembly SS 500 µl Complete Assembly, Stainless Steel - Cartridge and Housing Tee Assembly SS 350 µl Tee Assembly SS 500 µl Static s / Assemblies Port Size: CPI 350µL or 500µL Cartridge FLOWLOCK INPUT PORT FLOWLOCK OUTPUT PORTS (TWO OR THREE) Cartridges / Housings Stainless Steel Biocompatible PEEK - HP is not available Cartridge PEEK 350 µl Cartridge PEEK 500 µl Analytical Flow Series PEEK Housing Cartridge SS 350 µl Cartridge SS 500 µl Analytical Flow Series SS Housing Tee Analytical Flow Series SS Housing Tee Analytical Flow Series SS Housing B B B 53

13 Static s High Flow Series Stainless Steel, Biocompatible PEEK * Please add HP to end of part number for High Pressure application Complete Assembly - Cartridge and Housing Stainless Steel Biocompatible PEEK - HP is not available Assembly SS 800 µl Assembly SS 1.0 ml Assembly SS 1.5 ml Assembly PEEK 800 µl Assembly PEEK 1.0 ml Assembly PEEK 1.5 ml B B B Assemblies Port Size: CPI ( Standard HPLC Fittings for 1/16 Tube) 800uL, 1.0mL OR 1.5mL CARTRIDGE 800µL, 1.0mL or 1.5mL Cartridge ASI I 2.68 FLOWLOCK INPUT / OUTPUT ONE EACH END Analytical Scientific Instruments, Inc.

14 Complete Assembly, Stainless Steel - Cartridge and Housing Tee Assembly SS 800 µl Tee Assembly SS 1.0 ml Tee Assembly SS 1.5 ml Complete Assembly, Stainless Steel - Cartridge and Housing Tee Assembly SS 800 µl Tee Assembly SS 1.0 ml Tee Assembly SS 1.5 ml Static s / Assemblies Port Size: CPI 800uL, 1.0mL OR 1.5mL CARTRIDGE 800µL, 1.0mL or 1.5mL Cartridge ASI I 2.60 FLOWLOCK OUTPUT PORTS (TWO OR THREE) FLOWLOCK INPUT PORTS 1.43 FLOWLOCK INPUT PORT Cartridges / Housings Stainless Steel Biocompatible PEEK - HP is not available Cartridge SS 800 µl Cartridge SS 1.0 ml Cartridge SS 1.5 ml High Flow Series SS Housing Tee High Flow Series SS Housing Tee High Flow Series SS Housing Cartridge PEEK 800 µl Cartridge PEEK 1.0 ml Cartridge PEEK 1.5 ml High Flow Series PEEK Housing B B B B 55

15 Static s Combo Micro Series Stainless Steel, Biocompatible PEEK * Please add HP to end of part number for High Pressure application Order Instruction You can choose any 2, 3 or 4 cartridge combination from 0.5 µl, 1 µl, 2 µl, 5 µl, 10 µl and 25 µl mixer cartridges to create the desired volume to fit your application. The minimum volume you can create will be 2 x 0.5 µl cartridge combination and the maximum volume will be 4 x 25 µl cartridge combination. You will be able to create almost the exact volume you need within that range. Example) For a 4 mixer assembly if you choose 1 x 5 µl, 1 x 10 µl and 2 x 25 µl combination, you will get 65 µl volume mixer. You can specify as below when you order: Complete Assembly SS: Complete Assembly PEEK: B Assemblies Port Size: CPI ( Standard HPLC Fittings for 1/16 Tube) FLOWLOCK INPUT / OUTPUT ONE EACH END 56 Analytical Scientific Instruments, Inc.

16 Static s Complete Assembly - 2, 3 or 4 Cartridge Combination and Housing Stainless Steel Biocompatible PEEK - HP is not available Combo Micro 2 Assembly SS Combo Micro 3 Assembly SS Combo Micro 4 Assembly SS Combo Micro 2 Assembly PEEK Combo Micro 3 Assembly PEEK Combo Micro 4 Assembly PEEK 471-x-x 481-x-x-x 491-x-x-x-x 471-x-xB 481-x-x-xB 491-x-x-x-xB The application and Use of ASI Combo Micro Static s Chromatographers seeking to optimize mixing volumes at flow rates below 100 µl/min. now have the ability to customize a mixing volume which is perfectly tuned to match the separation system. Choosing the right mixing volume is always a compromise between absolute noise reduction, delay volume, and gradient distortion. Ideally the optimum mixing volume should be determined experimentally by evaluating several mixing volumes to determine which provides optimum chromatographic performance. For the separation below at 50 µl/min., a 50 µl mixer contributes too much delay volume and gradient distortion. The ASI Combo 35 µl (25 µl + 10 µl) mixer provides just the right balance between noise reduction and gradient performance. mvolts Combo Micro Static Performance at 50µL/min. 5 4 Combo Micro 35µL 3 2 ASI Standard In-line 50µL HPLC System Configuration: ASI Model 500G Micro Head Detection: UV214 Varian Star Data Acuisition HPLC Conditions: MP: A = H2O, B = H2O doped with 0.05% red dye Flow Rate: 50µL/min. Gradient 0 to 100% B in 10% Steps Minutes Minutes 57

17 Static s Static Application Notes Selecting the Right Size Cartridge ASI offers static mixers with volumes that range from 0.5 microliters to 1,500 microliters. Choosing the right size mixer is a trade off between delay volume, mixing noise, step gradient definition and chromatographic performance. Please refer to the Charts on pages 60 and 63 for more information on gradient accuracy as a function of flow rate and mixing volume. The following observations will provide some guidelines to help choose the right size mixer. Ø Ø Ø Ø For any given flow rate, the more the mixing volume the better the mixing, and the lower the baseline noise. The smaller the mixing volume, the better the definition and sharpness of linear gradients. Multi-pump high pressure gradient systems typically reuire far less mixing volume than low pressure single pump gradient systems when running linear gradients. A 150 µl in-line static mixer can be added after a dynamic or static onboard mixer to further reduce mixing noise. Multi-pump High Pressure Gradient Systems Linear Gradients If a larger mixing volume can be tolerated for a particular flow rate, the larger the volume will lower the mixing noise. The upper limits to mixing volume will be the maximum delay time that can be tolerated, and possible distortion (tailing) of the gradient at the beginning and end of the gradient. The lower limit will be defined by the amount of mixing noise that can be tolerated. Please refer to table 1 (page 59) for specific recommendations. or Steady State Composition Always select the largest volume that will still provide an acceptable delay volume. In general, the more mixing volume, the better the mixing will be. For most pump systems, a 150 µl cartridge will provide adeuate mixing. Examples of this type of pump system include: Shimadzu LC-10AD and LC-10ADvp Beckman System Gold Gilson Model 305 Agilent Model Analytical Scientific Instruments, Inc.

18 Static s Single-pump Low Pressure Mixing Gradient Systems Linear Gradients These systems generally reuire more mixing volume to perform linear gradients than multipump high pressure systems. The following will explain why this is the case. In a low pressure system the composition can only be changed once every pump stroke. Since the pump stroke volume of most pumps is 100 µl, and it takes a mixer volume that is about 3 times the batch volume to provide adeuate mixing, we need 350 µl of mixer volume, at least, to do adeuate mixing. More insoluble combinations may reuire even more mixing volume. In general, choose the largest size mixer cartridge that will still provide an acceptable delay volume. For most applications this will be at least 350 µl. or Steady State Composition Always select the largest volume that will still provide an acceptable delay time. In general, the more mixing volume, the better the mixing will be. For most applications this will be at least 350 µl. Examples of this type of pump system include: Agilent 1090 Perkin Elmer series 200 TSP Spectra Vision Waters model 626 Varian Star 9000 Cartridge Selection Guide for High Pressure Mixing Linear Gradients - High Pressure Mixing Larger mixing volume can be tolerated for a particular flow rate, with the larger the volume the lower the mixing noise. The upper limits to mixing volume will be the maximum delay time that can be tolerated, and possible distortion (tailing) of the gradient at the beginning and end of the gradient. The lower limit will be defined by the amount of mixing noise that can be tolerated. The following cartridge volumes are a compromise between these two limits. Table 1. Flow 0-5 micro liter/min Cartridge Volum e 5 micro liter 59

19 Static s Static Comparison mvolts Less Noise - Lower Detection Limits No - Valco Tee & 10 µl Tubing mvolts The Lee - 10 µl mvolts ASI HyperShear - 10 µl C HPLC System Configuration: ASI Model 500G Gradient System ABI Model 785 on-column Varian Star Data Acuisition HPLC Conditions: MP: A = H2O, B = H2O/Acetone Flow Rate: 50µL/min. 50% B Pressure: 1,700 PSI with ASI resistor cartridge Minutes Superior Mixing with Less Dispersion mvolts No - Valco Tee & 10 µl Tubing HPLC System Configuration: Above HPLC Conditions: Above Isocratic Flow Rate: 50µL/min. 100% A One inlet port plugged Injection: 600nL Acetone ASI HyperShear - 10 µl C The Lee - 10 µl Minutes 60 Analytical Scientific Instruments, Inc.

20 Static s Agilent Static Optimization mvolts -2.2 Micro Flow Step Gradients No ASI HyperShear - 50 µl Agilent - 80 µl Minutes HPLC System Configuration: Agilent 1100 Pump Packed bed mixer (approximate volume: 500µL) replaced with union and/or ASI HyperShear Static Varian Star Data Acuisition HPLC Conditions: MP: A = H2O, B = ACN doped with 0.01% Acetone Flow Rate: 250µL/min. Pressure: 2,000 PSI with ASI resistor cartridge Gradient: 0.0 = off%b 2.5 = 10%B 7.4 = 10%B 7.5 = 20%B 12.4 = 20%B 12.5 = 0.0%B and hold mvolts Analytical Flow Step Gradients No or BP ASI HyperShear - 50 µl ASI HyperShear µl ASI HyperShear µl ASI HyperShear µl ASI HyperShear µl Agilent Packed Bed Minutes HPLC System Configuration: Above Agilent 1100 Pump Packed bed mixer (approximate volume: 500µL) replaced with union and/or ASI HyperShear Static Varian Star Data Acuisition HPLC Conditions: MP: A = H2O, B = IPA doped with 0.01% Acetone Flow Rate: 1.0mL/min. Pressure: 2,000 PSI with ASI resistor cartridge Gradient: Above 61

21 Static s Shimadzu Static Optimization Micro Flow Step Gradients Shimadzu µl Noise (mm) ASI HyperShear µl Composition (%B) Micro Flow Step Gradients mvolts 18 Shimadzu µl ASI HyperShear µl Minutes HPLC System Configuration: Shimadzu: LC-10ADvp : Connected at Shimadzu Tee Detector: Knauer HPLC Conditions: MP: A = H2O, B = H2O + 35% 2-Propanol % Acetone Flow Rate: 250µL/min. Pressure: 2,000 PSI with ASI resistor cartridge 62 Analytical Scientific Instruments, Inc.

22 Static s Shimadzu Static Optimization Noise (mm) Analytical Flow Step Gradients Composition (% B) ASI 250ul ASI 350ul Shimadzu 500ul HPLC System Configuration: Shimadzu: LC-10ADvp : Connected at Shimadzu Tee Detector: Knauer HPLC Conditions: MP: A = H2O, B = H2O + 35% 2-Propanol % Acetone Flow Rate: 1.0mL/min. Pressure: 2,000 PSI with ASI resistor cartridge mvolts 3.4 Isocratic Mixing Noise Shimadzu µl ASI HyperShear µl ASI HyperShear µl HPLC System Configuration: Shimadzu: LC-10ADvp : Connected at Shimadzu Tee Detector: Knauer HPLC Conditions: MP: A = H2O, B = H2O + 35% 2-Propanol % Acetone Flow Rate: 1.0mL/min. Pressure: 2,000 PSI with ASI resistor cartridge Minutes Minutes 63

23 Dynamic s HyperShear TM Dynamic s HyperShear Dynamic, 1.0 ml HyperShear Combination Static/Dynamic, 150 µl, 250 µl or 350 µl HyperShear TM Dynamic Features: Vortex sheer mixing that is twice as efficient as conventional stir bar mixers Models with fixed or adjustable mixing volumes Compact design allows easy integration into any HPLC system / Complete Assembly Tee Dynamic Assembly 150 µl Tee Dynamic Assembly 150 µl Tee Dynamic Assembly 250 µl Tee Dynamic Assembly 250 µl Tee Dynamic Assembly 350 µl Tee Dynamic Assembly 350 µl Tee Dynamic Assembly 1.0 ml Tee Dynamic Assembly 1.0 ml 120 VAC A 220 VAC U 120 VAC A 220 VAC U 120 VAC A 220 VAC U 120 VAC A 120 VAC A 64 Analytical Scientific Instruments, Inc.