Mitos P-Pump Accessories

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1 Mitos P-Pump Accessories product datasheet page Flow Resistors 3 Flow Resistor Selection 6 Flow Resistor Kit 7 Mitos P Pump Vessel Holder Kit 9 Standard Vessel Compatibility Chart 11 Mitos P Pump Starter Kit 13 Pneumatic Connector Kit 14 Mitos P Pump 3-way Chamber Kit 15 Mitos P Pump 3-way Vessel Holder Kit 16 Mitos P Pump Remote Chamber Mitos P-Pump Remote Chamber Mitos P-Pump Remote Chamber with 3-way Accessories 19 Fluid Tube (1/16 ) to Pneumatic Tube (6mm) Adaptor 20 Dip Tube Fitting 20 USB to RS232 Adaptor Cable 21 MAR Page 1 of 22

2 Part name Part number Flow Resistors Flow Resistor Kit Mitos P-Pump Vessel Holder Kit Mitos P-Pump Starter Kit Pneumatic Connector Kit Mitos P-Pump 3-way Chamber Lid Mitos P-Pump 3-way Vessel Holder Kit Mitos P-Pump Remote Chamber Mitos P-Pump Remote Chamber Mitos P-Pump Remote Chamber with 3-way Accessories Dip Tube Fitting Fluid Tube (1/16 ) to Pneumatic Tube (6mm) Adaptor RS232 Serial Extension Cable RS232 to USB Adaptor Module MAR Page 2 of 22

3 Flow Resistors (Part No ) Flow Resistors are high tolerance lengths of PEEK covered fused silica tubing that provide a known flow rate at a selected pressure. They can be used to insert additional flow resistance into a system and are designed for use with the Mitos P-Pump range. A Flow Resistor enables very low flow rates to be achieved when connecting to a Mitos P-Pump to systems of low fluidic resistance. For applications such as droplet generation, a Flow Resistor can be used to prevent flow rate instability due to the varying back pressure generated by using immiscible fluids of different viscosities. Dolomite offers a range of Flow Resistors to optimise your microfluidic system Flow Resistor F1 system Flow resistor connected between a Mitos P-Pump and system MAR Page 3 of 22

4 Benefits Allows the flow rate range of the Mitos P-Pump to be maximized Enables the user to operate at very low flow rates with the Mitos P-Pump Enables the use of very low viscosity liquids (e.g. gases) with the Mitos P-Pump Prevents flow rate instability when using fluids of different viscosities Two Flow Resistors can be used to provide equal flow splitting into two channels Quick and easy to connect using included fittings Excellent chemical compatibility Specifications Specification F0.1 Approx. flow rate generated by water pumped at 1bar pressure F F F F F F µl/min 0.3µl/min 1µl/min 3µl/min 10µl/min 30µl/min 100µl/ min Max flow rate 1 1µl/min 3µl/min 10µl/min 30µl/min 100µl/ min 300µl/ min 1000µl/ min Min flow rate 2 Internal diameter Internal diameter tolerance Outer diameter Wetted materials Sheath materials 0.002µl/ min 0.006µl/ min 0.02µl/mi n 0.06µl/mi n 0.2µl/min 0.6µl/min 2µl/min 25µm 50µm 75µm 100µm ± 1µm ± 2µm ± 3µm ± 3µm 1/16 (1.59mm) Fused silica Polyimide (inner sheath) PEEK (outer sheath) 1) Maximum flow rate is the approximate flow rate produced with water when the Mitos P- Pump is set to 10bar and there is little or no fluidic resistance downstream of the flow restrictor. 2) Minimum flow rate is the approximate flow rate produced with water when the Mitos P- Pump is set to 20mbar and there is little or no fluidic resistance downstream of the flow restrictor. MAR Page 4 of 22

5 Flow accessories The Flow Resistors are supplied with the following flow accessories: 2 x Compact Head Fittings (ETFE) with 1/4-28 thread for connection to 1/16 OD pipe, can be used with the Flangeless Ferules. 2 x Flangeless Ferules (ETFE) for connection to 1/16 OD pipe. To be used with the Compact Head Fittings. 2 x Straight Female Couplings (Polypropylene) with female to female 1/4-28 threads. The couplings are used for making connection between Compact Head Fittings. 1 x Ferule (PCTFE) with Integrated Filter (stainless steel, 2 µm pore size). To be used with 1/16 OD pipe. Each Flow Resistor is supplied with these flow accessories to enable the Flow Resistor to be connected in-line. The included filter can be used to prevent blockage of the small internal diameter of the flow resistor. Flow resistors can also be directly connected to a microfluidic chip using the Multiflux Linear Connectors. Each Flow Resistor is supplied with the above flow accessories to enable the Flow Resistor to be connected in-line. MAR Page 5 of 22

6 Dynamic viscosity of selected fluid (cp) The Dolomite Centre Ltd Flow Resistor Selection The Flow Resistor should be selected based on the desired flow rate and the viscosity of the selected fluid using the table below. This assumes that the flow resistance of the system is low in comparison with the Flow Resistor. 0.1 e.g. propane gas at 20ºC 0.3 e.g. acetone at 20 ºC 1 e.g. water at 20ºC 3 blood at 37ºC 10 25% wt CaCl sol. at 20ºC 30 vegetable oil at 40ºC 100 light machine oil at 20ºC 0.1 Flow rate range Desired flow rate at 1bar pressure (µl/min) 0.3 Flow rate range Flow rate range Flow rate range Flow rate range Flow rate range Flow rate range F0.1 F0.3 F1 F3 F10 - F0.1 F0.3 F1 F3 F10 F30 F0.1 F0.3 F1 F3 F10 F30 F100 F0.3 F1 F3 F10 F30 F100 F1 F3 F10 F30 F100 F3 F10 F30 F100 F10 F30 F100 Operate without flow resistors MAR Page 6 of 22

7 Flow Resistor Kit (Part No ) Designed for the Mitos P-Pump range the Flow Resistor Kit can be used to insert additional flow resistance into a system. The Flow Resistors enable very low flow rates to be achieved when connecting to a Mitos P-Pump to systems of low fluidic resistance. For applications such as droplet generation, a Flow Resistor can be used to prevent flow rate instability due to the varying back pressure generated by using immiscible fluids of different viscosities. Flow Resistor Kit (Part No ) Benefits Allows the flow rate range of the Mitos P-Pump to be maximised Enables the user to operate at very low flow rates with the Mitos P-Pump Enables the use of very low viscosity liquids (e.g. gases) with the Mitos P-Pump Prevents flow rate instability when using fluids of different viscosities Quick and easy to connect using included fittings Excellent chemical compatibility MAR Page 7 of 22

8 The Flow Resistor Kit contains the following flow resistors: Item Description Qty 1 Flow Resistor F1 Min flow rate: 0.02µl/min, Max flow rate: 10µl/min 1 2 Flow Resistor F3 Min flow rate: 0.06µl/min, Max flow rate: 30µl/min 1 3 Flow Resistor F10 Min flow rate: 0.2µl/min, Max flow rate: 100µl/min 1 4 Flow Resistor F30 Min flow rate: 0.6µl/min, Max flow rate: 300µl/min 1 5 Flow Resistor F100 Min flow rate: 2µl/min, Max flow rate: 1000µl/min 1 Each flow resistor kit also contains 5 sets of flow accessories to enable the flow resistors to be connected in-line and the fluids to be filtered before entry into the flow resistor. Each Flow Resistor is supplied with the above flow accessories to enable the Flow Resistor to be connected in-line. MAR Page 8 of 22

9 Mitos P-Pump Vessel Holder Kit (Part No ) The Mitos P-Pump Vessel Holders Kit contains 4 vessel holders to enable the Mitos P-Pump range to be used with a wide range of standard and non-standard fluid vessels. These include centrifuge tubes, auto sampler vials, scintillation vials, microreaction vials and other general purpose vials. The Vessel Holders can be easily positioned inside the pressure chamber and are quick to change. Left: Mitos P-Pump with vessel holder 2 Right: Mitos P-Pump with vessel holder 4 Vessel Holder 1 Vessel Holder 2 Vessel Holder 3 Vessel Holder 4 MAR Page 9 of 22

10 Benefits Accommodate a wide range of standard and non-standard fluid vessels Ensures the Mitos P-Pump can be used with preferred vessel Minimize sample waste by pumping directly from your container Enable use of micro vials for low sample volumes Specification Vessel Holder 1 Vessel Holder 2 Vessel Holder 3 Vessel Holder 4 1 Hole diameter D1 (mm) Height H1 (mm) Base diameter D2 (mm) Height H2 (mm) 5 5 N/A N/A 5 Material Stainless Steel MAR Page 10 of 22

11 Standard Vessel Compatibility Chart The compatibility chart contains some examples of commonly used vessels, which require a vessel holder for use with the Mitos P-Pump range. Standard Vessel Type Vessel Holder Required Abgene deep well cluster tube 0.3 ml 1 Abgene deep well cluster tube 0.65 ml 1 Abgene deep well cluster tube 1.2 ml 1 Abgene flat cap tube 0.2 ml 1 Autosampler vial 12 x 32 mm 1 Autosampler vial 4 ml 1 Chase culture tube 10 x 75 mm 4 Chase culture tube 15 x 85 mm 3 Chase scintillation vial 7 ml 2 Chase scintillation vial 20 ml 3 Chase scintillation vial 30 ml 3 Chromacol universal storage vial 8ml 2 MAR Page 11 of 22

12 Eppendorf micro centrifuge tube 0.5 ml 1 Eppendorf micro centrifuge tube 1.5 ml 4 Eppendorf micro centrifuge tube 2.0 ml 4 Micro reaction vessel 0.1 ml 2 Micro reaction vessel 0.3 ml 2 Micro reaction vessel 1 ml 2 Micro reaction vessel 2 ml 2 Micro reaction vessel 3 ml 3 Micro reaction vessel 5 ml 3 Nalgene cryogenic vial 1.2 ml 1 Nalgene cryogenic vial 2 ml 1 Nalgene cryogenic vial 5 ml 1 Nalgene oak ridge centrifuge tube 10 ml 3 Nalgene sample vial 5 ml 3 Nalgene sample vial 12 ml 3 Nalgene sample vial 18 ml 3 Wheaton sample glass vial 2 ml 1 Wheaton sample glass vial 4 ml 1 Wheaton sample glass vial 6 ml 2 Wheaton v vial 0.3 ml 1 Wheaton v vial 1 ml 1 Wheaton v vial 2 ml 2 Wheaton v vial 3 ml 2 MAR Page 12 of 22

13 Mitos P-Pump Starter Kit (Part No ) The Mitos P-Pump Starter Kit contains a selection of vessels to enable sample liquids to be introduced into the range of Mitos P-Pumps. It also contains a selection of tubing and fittings to enable fluidic connection to be made between the P-Pump and your microfluidic system. Mitos P-Pump Starter Kit Benefits Enables the user to begin test work with the Mitos P-Pump immediately Contains a selection of vessels Contains a selection of tubing and fittings The Mitos P-Pump Starter Kit contains: Item Description Qty 1 30ml glass vessel 30ml, Ø28 x H83mm, R3 screw neck with cap, class 1 5 hydrolytic extraction neutral borosilicate glass 2 20ml high recovery 20ml, Ø28 x H67mm, screw thread with cap, class 1 2 glass vessel hydrolytic extraction neutral borosilicate glass, tapered reservoir for high sample recovery 3 FEP tubing 0.25mm ID, 1/16 (1.6 mm) OD, 10m length 1 4 FEP tubing with 0.25mm ID, 1/16 (1.6 mm) OD, 1m length, ¼ -28 threaded 1 attached fitting fitting with PTFE gripper attached to one end 5 ¼ -28 fitting ¼ -28 threaded nut and ferule for 1/16 (1.6mm) OD tubing 5 6 ¼ -28 union ¼ -28 female to female threaded union 1 7 Tube cutter Double bevelled cutting blade produces clean 90º cut on polymer tubing up to 2mm OD 1 MAR Page 13 of 22

14 Pneumatic Connector Kit (Part No ) The Pneumatic Connector Kit contains a selection of fittings and tubing to enable connection of your compressed air or gas supply to the Mitos P-Pumps. Pneumatic Connector Kit Benefits Enables connection of most compressed air or gas supplies to the Mitos P-Pump Contains a selection of fittings and tubing The Pneumatic Connector Kit contains: Item Description Qty 1 Pneumatic tubing 6mm OD, 4mm ID, 5m length, polyurethane 1 2 Straight connector 6mm Push-in tube tube connector for 6mm OD tube 1 3 Reducer 6 4mm 6mm male to 4mm female push-in tube connector 1 4 Reducer 8 6mm 8mm male to 6mm female push-in tube connector 1 5 T connector 6mm 6mm push-in 3-way tube connector 3 6 Adaptor 6mm to 1/4 6mm push-in tube connector to 1/4 BSPT male thread 1 BSPT 7 Adaptor 6mm to 1/8 6mm push-in tube connector to 1/8 BSPP female thread 1 BSPP 8 Adaptor 1/4 NPT to 1/4 1/4 NPT male thread to 1/4 BSPP female thread 1 BSPP 9 Adaptor 1/4 to 1/8 BSPT 1/4" push-in tube connector to 1/8 BSPT male thread 1 MAR Page 14 of 22

15 Mitos P-Pump 3-way Chamber Lid (Part No ) The Mitos P-Pump 3-way Chamber Lid can be used to pump 3 liquids simultaneously from the Mitos P-Pump (Part No ). This provides a low cost method of delivering 3 reagents to a microfluidic system with pulseless pressure pump technology. Also available, is a Mitos P-Pump 3-way Vessel Holder Kit (Part No ) to enable 3 reagent vessels to be inserted into the chamber with ease. Mitos P-Pump 3-way Chamber Lid Mitos P-Pump with 3-way Chamber Lid and 3-way Vessel Holder Benefits Provides a low cost solution for 3-channel pumping with the Mitos P-Pump Push-and-rotate mechanism that is quick to open and close Safety features to ensure chamber is locked when pressurized Contains plugs to enable ports to be blocked when not required Specification Value 1 Number of ports 3 2 Chamber dimensions Ø30 x H 88mm 3 Dip tube size 1/16 OD; 250µm ID 4 Wetted materials User selected vessel and dip tube (e.g. glass and PTFE) 5 Number of plugs included 10 MAR Page 15 of 22

16 Mitos P-Pump 3-way Vessel Holder Kit (Part No ) The Mitos P-Pump 3-way Vessel Holder Kit is designed to accommodate 3 micro tubes in the Mitos P-Pump (Part No ). Included in the kit are compatible micro tubes with 1.5 ml volume. When used in conjunction with the 3-way Chamber Lid (Part No ), it provides a low cost method of delivering 3 reagents to a microfluidic system with pulseless pressure pump technology. Mitos P-Pump 3-way Vessel Holder 1.5 ml micro tube Mitos P-Pump with 3-way Chamber Lid and 3-way Vessel Holder Benefits Improves ease of use when using the Mitos P-Pump for 3-channel pumping Enables P-Pump to be used with 3 micro tubes Quick to install and remove Locating feature to align micro tubes with outlet ports in chamber lid Specification Value 1 Vessel holder hole diameter D1 (mm) 11 2 Vessel holder height H1 (mm) 15 3 Vessel holder base diameter D2 (mm) 29 4 Vessel holder material Stainless Steel 5 Number of micro tubes 20 6 Micro tube volume (ml) Micro tube material Polypropylene MAR Page 16 of 22

17 Mitos P-Pump Remote Chamber 400 (Part No ) The Mitos P-Pump Remote Chamber 400 is a 10bar stainless steel pressure chamber for use with the Mitos P-Pump (Part No ). It enables greater sample volumes up to 400ml to be used, so that continuous pumping for longer periods of time is possible. With the precise pressure-driven pumping mechanism of the Mitos P-Pump, the flow from the chamber is highly stable and pulsefree. The remote chamber 400 is simple to connect and provides clear viewing of the sample liquid. For applications requiring temperature control and stirring, the remote chamber can be placed onto a hotplate/magnetic stirrer Mitos P-Pump Remote Chamber 400 Mitos P-Pump (Part No ) with Remote Chamber 400 Benefits Expands P-Pump capacity to liquid vessels up to 400ml Continuous pumping for long periods of time Option for heating and magnetic stirring Easy to view sample liquid levels Simple to connect to the P-Pump for precise pressure control 250 ml DURAN vessel included Excellent chemical compatibility Specification Value 1 Pressure range 0 10bar (0 145psi) gauge 2 Temperature limit 50ºC 2 Chamber dimensions Ø82 x H 140mm 3 Sample volume up to 400ml (with 400ml tall form beaker) 4 Dip tube size 1/16 OD; 250µm ID 5 Product size Ø148 x H 200mm 6 Product weight 5.5kg 7 Chamber materials Stainless Steel, Glass, Viton, PEEK 8 Wetted materials User selected vessel and dip tube (e.g. glass and PTFE) Note: Pressure control performance will be as indicated in the P-Pump specification except response and settling times will be slower due to the increased volume MAR Page 17 of 22

18 Mitos P-Pump Remote Chamber 30 (Part No ) The Mitos P-Pump Remote Chamber 30 is a 0 10bar lockable glass pressure chamber for use with the Mitos P-Pump Remote (Part No ) and Mitos P-Pump Remote Basic (Part No ). It is easy to access and can accommodate samples from 100µl to 30ml. The Mitos P-Pump Remote Chamber 30 connects to a Mitos P-Pump Remote via a simple push-and-click action. For applications requiring temperature control and stirring, the remote chamber can be placed onto a hotplate, magnetic stirrer or placed in an incubator. Left: Mitos P-Pump Remote Chamber 30 (Part No ) Below: Mitos P-Pump Remote Chamber 30 (Part No ) connected to Mitos P-Pump Remote (Part No ) Benefits Sample volumes from 100µl to 30ml Option for heating and magnetic stirring Easy to view sample liquid levels Quick and easy fitting to connect to Mitos P-Pump Remote Excellent chemical compatibility MAR Page 18 of 22

19 Mitos P-Pump Remote Chamber with 3-way Accessories (Part No ) The Mitos Remote Chamber with 3-way Accessories is the Mitos P-Pump Remote Chamber 30 (Part No ) with Mitos P-Pump 3-way Chamber Lid (Part No ) and Mitos P-Pump 3-way Vessel Holder Kit (Part No ), it provides a low cost method of delivering 3 reagents to a microfluidic system with pulseless pressure pump technology Mitos P-Pump Remote Chamber Mitos P-Pump Basic with 3-way Chamber Lid way Vessel Holder Kit Benefits Sample volumes from 100µl to 30ml Option for heating and magnetic stirring Easy to view sample liquid levels Quick and easy fitting to connect to Mitos P-Pump Remote Excellent chemical compatibility MAR Page 19 of 22

20 Dip Tube Fitting (Part No ) Designed for use with the Mitos P-Pump (Part No ), the Dip Tube Fitting allows users to easily connect a dip tube into a fluid vessel on the Mitos P-Pump. The fitting features an in-built strain relief and is compatible with 1/16 OD tubing and 1/4-28 flat bottom ports. Dip Tube Fitting (Part No ) Fluid Tube (1/16 ) to Pneumatic Tube (6mm) Adaptor (Part No ) The Fluid Tube (1/16 ) to Pneumatic Tube (6mm) Adaptor is used to convert 6mm air lines to 1/16 fluid tube, which is the tubing size required for connection to all Mitos P- Pumps. Fluid Tube (1/16 ) to Pneumatic Tube (6mm) Adaptor (Part No ) MAR Page 20 of 22

21 USB to RS232 Adaptor Cable For PCs that do not support an RS232 serial interface, Dolomite offers a RS232 Serial Extension Cable (Part No ) and a RS232 to USB Adaptor Module (Part No ) allowing users to connect their Mitos P-Pump Basic (Part No ) or Mitos P-Pump Remote Basic (Part No ) to a PC. Right: RS232 Serial Extension Cable (Part No ) Left: RS232 to USB Adaptor Module (Part No ) MAR Page 21 of 22

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