Stability Axial Compression Technology Application Ordering Information

Transcription

1 42 YMC-Actus

2 YMC-Actus 43 YMC-Actus Contents Stability Axial Compression Technology Application Ordering Information Introduction Fast semi-preparative chromatography Semi-preparative chromatography is the link between analytical HPLC and preparative LC. Even though the chromatographic systems used for semi-preparative LC are not as large as preparative LC systems, the objectives remain the same: Purification and isolation of maximum sample quantity Savings in time and costs. With YMC-Actus, time is on your side! * Application data by courtesy YMC Co., Ltd.

3 44 YMC-Actus YMC-Actus Stability Rapid pressure changes under high-speed gradient conditions may lead to column degradation and loss of column performance. With YMC-Actus, a new hardware and packing technology has been applied to these semipreparative columns to provide a uniform packing density which results in a longer lifetime than conventional semi-preparative columns. In order to determine the quality of the packing, the column performance has been evaluated every 100 runs with a sequential high-throughput gradient. YMC-Actus columns offer outstanding efficiency without compromising resolution. Furthermore, YMC-Actus columns provide reliable results, even after exposure to severe, rapid gradient conditions and multiple injections. Increase column lifetime = Decrease of cost Conventional semi-preparative column Sequential gradient test Colum: 50 x 20 mm ID, 5 µm Eluent: A) water B) methanol Gradient: Flow rate: 50 ml/min Temp.: ambient 5% B (0-0.5 min), 5-95% B ( min), 95% B ( min), 5% B ( min) Column performance test Column: 50 x 20 mm ID, 5 µm Eluent: methanol / water (70/30) Flow rate: 10 ml/min Sample: Naphthalene

4 YMC-Actus 45 YMC-Actus Axial Compression Technology for Ultimate Separation YMC-Actus series columns are semi-preparative HPLC columns that have excellent column stability and efficiency as a result of applying axial compression technology. YMC-Actus series columns show high stability under high flow rate or steep gradient conditions which are desirable for milligram scale preparative HPLC of various compounds. 10% increase in bed density Compressing Flow Test conditions (high-speed and high-pressure) Column: 5 µm, 50 x 20 mm ID Eluent: A) water B) methanol Gradient: 5%-95% B Flow rate: 50 ml/min Pressure: ~ 17 MPa Long Lasting Columns! after 700 runs initial Conventional Column Packing without compression Flow Void and crack Degradation of performance after 300 runs initial Uniformly high density packing is necessary for highly efficient and stable HPLC columns. DAC (Dynamic Axial Compression) columns are widely used for preparative separation in pilot or production scale. This allows uniformly high density packing and prevents formation of voids. YMC-Actus series columns have been developed by applying this Axial Compression Technology to semi-prep column production. The column bed is compressed appropriately when attaching the inlet end assembly of the newly designed YMC-Actus hardware. It provides increased bed density (10% higher than conventional columns) and bed uniformity. * Application data by courtesy YMC Co., Ltd.

5 46 YMC-Actus YMC-Actus Excellent stability and efficiency under fast gradient conditions at high flow rate Glycyrrhizic acid in herb medicine* Analysis Purification Eluent: A) water / acetic acid (99/1) B) methanol / acetic acid (99/1) 20% B (0-2 min), 20-45% B (2-7 min), 45% B (7-10 min) Temperature: ambient Detection: UV at 260 nm Sample: water / methanol / acetic acid extract of commercially available herb medicine (0.1 g/ml) Available for high-throughput purification: Injection in DMSO 1. Ethenzamide 2. Clemastine fumarate 3. Ibuprofen Column: YMC-Actus Pro C18 RS (5 µm, 8 nm), 50 x 20 mm ID Eluent: A) water / TFA (100/0.1) B) acetonitrile / TFA (100/0.1) Gradient: 10-90% B (0-3 min), 90% B (3-5 min), 10% B (5-10 min) Flow rate: 20 ml/min Temperature: ambient Detection: UV at 270 nm Injection: 100 µl (0.2-2 mg/ml in DMSO) As shown in overlay chromatograms, YMC-Actus columns provide outstanding stability and reproducibility in the separation of pharmaceuticals dissolved in 100% DMSO, even after 1,000 injections under the test conditions. This makes YMC-Actus columns ideal for high-throughput purification in drug discovery.

6 YMC-Actus 47 YMC Actus Excellent separation of compounds with similar structure Capsaicinoids in red pepper* Analysis 50 x 4.6 mm ID, 5 µm 2.0 ml/min, 20 µl injection Phenomenex Luna C18 Waters XBridge C18 YMC-Pack Pro C18 RS Purification YMC-Actus Pro C18 RS, 50 x 20 mm ID 5 µm Nordihydrocapsaicin Capsaicin Dihydrocapsaicin 40 ml/min, 400 µl injection Purity >99.5% Eluent: A) methanol / water / TFA (50/50/0.1) B) methanol / TFA (100/0.1) 0-30% B (0-5 min), 30% B (5-10 min) Temperature: 25 C for 50 x 4.6 mm ID ambient for 50 x 20 mm ID Detection: UV at 280 nm Sample: methanol extract of a commercial red pepper (1 g / 3 ml) Outstanding separation of highly polar compounds Crude synthetic 30mer oligonucleotide* Analysis Hydrosphere C ml/min, 5 µl injection Purification 19 ml/min, 100 µl injection 50 x 4.6 mm ID, 5 µm 50 x 20 mm ID, 5 µm Impurities 30mer YMC-Actus Hydrosphere C18 Recovery 56% Eluent: A) 10 mm DBA-acetic acid (ph 6.0) / methanol (60/40) B) 10 mm DBA-acetic acid (ph 6.0) / methanol (20/80) 10-35% B (0-30 min) Temperature: ambient Detection: UV at 269 nm Sample: synthetic oligonucleotide (100 µm) purity>99% * Application data by courtesy YMC Co., Ltd.

7 48 YMC-Actus Ordering Information High durability semi-preparative columns Packing material Particle size [µm] Pore size [nm] Column size length x ID [mm] Product Code Triart C18 S-5 12 Pro C18 S-5 12 Hydrosphere C18 S-5 12 Pro C18 RS S-5 8 Triart C8 S-5 12 Pro C8 S x 20 TA12S WX 100 x 20 TA12S WX 150 x 20 TA12S WX 250 x 20 TA12S WX 50 x 30 TA12S WX 75 x 30 TA12S05-L530WX 100 x 30 TA12S WX 150 x 30 TA12S WX 250 x 30 TA12S WX 100 x 50 TA12S DX 150 x 50 TA12S DX 250 x 50 TA12S DX 50 x 20 AS12S WX 100 x 20 AS12S WX 150 x 20 AS12S WX 250 x 20 AS12S WX 50 x 30 AS12S WX 75 x 30 AS12S05-L530WX 100 x 30 AS12S WX 150 x 30 AS12S WX 250 x 30 AS12S WX 100 x 50 AS12S DX 150 x 50 AS12S DX 250 x 50 AS12S DX 50 x 20 HS12S WX 100 x 20 HS12S WX 150 x 20 HS12S WX 250 x 20 HS12S WX 50 x 30 HS12S WX 75 x 30 HS12S05-L530WX 100 x 30 HS12S WX 150 x 30 HS12S WX 250 x 30 HS12S WX 100 x 50 HS12S DX 150 x 50 HS12S DX 250 x 50 HS12S DX 50 x 20 RS08S WX 100 x 20 RS08S WX 150 x 20 RS08S WX 250 x 20 RS08S WX 50 x 30 RS08S WX 75 x 30 RS08S05-L530WX 100 x 30 RS08S WX 150 x 30 RS08S WX 250 x 30 RS08S WX 100 x 50 RS08S DX 150 x 50 RS08S DX 250 x 50 RS08S DX 50 x 20 TO12S WX 100 x 20 TO12S WX 150 x 20 TO12S WX 250 x 20 TO12S WX 50 x 30 TO12S WX 75 x 30 TO12S05-L530WX 100 x 30 TO12S WX 150 x 30 TO12S WX 250 x 30 TO12S WX 100 x 50 TO12S DX 150 x 50 TO12S DX 250 x 50 TO12S DX 50 x 20 OS12S WX 100 x 20 OS12S WX 150 x 20 OS12S WX 250 x 20 OS12S WX 50 x 30 OS12S WX 75 x 30 OS12S05-L530WX 100 x 30 OS12S WX 150 x 30 OS12S WX 250 x 30 OS12S WX 100 x 50 OS12S DX 150 x 50 OS12S DX 250 x 50 OS12S DX

8 YMC-Actus 49 Ordering Information High durability semi-preparative columns Packing material Particle size [µm] Pore size [nm] Column size length x ID [mm] Product Code ODS-A S-5 12 ODS-AQ S-5 12 YMCbasic S-5 50 x 20 AA12S WX 100 x 20 AA12S WX 150 x 20 AA12S WX 250 x 20 AA12S WX 50 x 30 AA12S WX 75 x 30 AA12S05-L530WX 100 x 30 AA12S WX 150 x 30 AA12S WX 250 x 30 AA12S WX 100 x 50 AA12S DX 150 x 50 AA12S DX 250 x 50 AA12S DX 50 x 20 AQ12S WX 100 x 20 AQ12S WX 150 x 20 AQ12S WX 250 x 20 AQ12S WX 50 x 30 AQ12S WX 75 x 30 AQ12S05-L530WX 100 x 30 AQ12S WX 150 x 30 AQ12S WX 250 x 30 AQ12S WX 100 x 50 AQ12S DX 150 x 50 AQ12S DX 250 x 50 AQ12S DX 50 x 20 BA99S WX 100 x 20 BA99S WX 150 x 20 BA99S WX 250 x 20 BA99S WX 50 x 30 BA99S WX 75 x 30 BA99S05-L530WX 100 x 30 BA99S WX 150 x 30 BA99S WX 250 x 30 BA99S WX 100 x 50 BA99S DX 150 x 50 BA99S DX 250 x 50 BA99S DX Guard cartridges Packing material Particle size [µm] Pore size [nm] Column size length x ID [mm] Product Code Triart C18 S-5 12 Pro C18 S-5 12 Hydrosphere C18 S-5 12 Pro C18 RS S-5 8 Pro C8 S-5 12 ODS-A S-5 12 ODS-AQ S-5 12 S Triart Prep C18-S S Triart Prep C8-S S Omega S-10 YMCbasic S-5 10 x 20 TA12S CC 10 x 30 TA12S CC 10 x 20 AS12S CC 10 x 30 AS12S CC 10 x 20 HS12S CC 10 x 30 HS12S CC 10 x 20 RS08S CC 10 x 30 RS08S CC 10 x 20 OS12S CC 10 x 30 OS12S CC 10 x 20 AA12S CC 10 x 30 AA12S CC 10 x 20 AQ12S CC 10 x 30 AQ12S CC 10 x 20 TAS12S CC 10 x 30 TAS12S CC 10 x 20 TAS12S CC 10 x 30 TAS12S CC 10 x 20 TOS20S CC 10 x 30 TOS20S CC 10 x 20 OMG99S CC 10 x 30 OMG99S CC 10 x 20 BA99S CC 10 x 30 BA99S CC Guard cartridges holder: 20 mm guard column ID: XPCHSPW2; 30 mm guard column ID: XPCHSPW3 * Application data by courtesy YMC Co., Ltd.