ACQUITY Advanced Polymer Chromatography

Transcription

1 ACQUITY Advanced Polymer Chromatography Isocratic Solvent Manager Overview and Maintenance Guide / Revision B Copyright Waters Corporation 2014 All rights reserved

2 ii October 13, 2014, Rev. B

3 Copyright notice 2014 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER. The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com). Trademarks ACQUITY, Connections INSIGHT, Empower, UPLC, THE SCIENCE OF WHAT S POSSIBLE. and Waters are registered trademarks, and Advanced Polymer Chromatography, APC, ecord, Empower, LAC/E are trademarks of Waters Corporation. PEEK is a trademark of Victrix PLC. Teflon, Tefzel, and Vespel are registered trademarks of E.I. Dupont de Nemours & Company. Nitronic Torx is a registered trademark of Textron, Inc. Microsoft, Windows, Word, Excel, and PowerPoint are registered trademarks of Microsoft Corporation in the United States and/or other countries. Other registered trademarks or trademarks are the sole property of their owners. Customer comments Waters Technical Communications organization invites you to report any errors that you encounter in this document or to suggest ideas for otherwise improving it. Help us better understand what you expect from our October 13, 2014, Rev. B iii

4 documentation so that we can continuously improve its accuracy and usability. We seriously consider every customer comment we receive. You can reach us at Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information: Contacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide. Visit Telephone and fax From the USA or Canada, phone , or fax For other locations worldwide, phone and fax numbers appear in the Waters Web site. Conventional mail Waters Corporation 34 Maple Street Milford, MA USA Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical, biological, or radiological hazards (or any combination thereof). You must know the potentially hazardous effects of all substances you work with. Always follow Good Laboratory Practice, and consult your organization s safety representative for guidance. iv October 13, 2014, Rev. B

5 Considerations specific to the p-ism High voltage hazard Warning: To avoid electric shock, do not remove the device s protective panels. The components within are not user-serviceable. FCC radiation emissions notice Changes or modifications not expressly approved by the party responsible for compliance, could void the users authority to operate the equipment. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. Canada spectrum management emissions notice This class A digital product apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe A est conforme à la norme NMB-003. Electrical power safety notice Do not position the instrument so that it is difficult to operate the disconnecting device. Safety hazard symbol notice Documentation needs to be consulted in all cases where the symbol is used to find out the nature of the potential hazard and any actions which have to be taken. Equipment misuse notice If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. October 13, 2014, Rev. B v

6 Safety advisories Consult Appendix A for a comprehensive list of warning and caution advisories. Operating this device When operating this device, follow standard quality-control (QC) procedures and the guidelines presented in this section. Applicable symbols Symbol Definition Manufacturer Authorized representative of the European Community Confirms that a manufactured product complies with all applicable European Community directives Australia C-Tick EMC compliant Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements Consult instructions for use Electrical and electronic equipment with this symbol may contain hazardous substances and should not be disposed of as general waste. For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions. vi October 13, 2014, Rev. B

7 Audience and purpose This guide is intended for personnel who install, operate and maintain the ACQUITY Advanced Polymer Chromatography system. It gives an overview of the technology and operation of the isocratic solvent manager. Intended use of the isocratic solvent manager Waters designed the isocratic solvent manager for use in advanced polymer chromatography applications for the molecular weight characterization of aqueous and organic polymers in solution. The isocratic solvent manager is not intended for use in diagnostic applications. Industrial scientific and medical classification Industrial scientific and medical classification: Industrial Scientific and Medical Group 1 Class B This classification has been assigned in accordance with IEC CISPR 11 Industrial scientific and medical instruments requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radio-frequency energy that is necessary for the internal functioning of the equipment. Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network. October 13, 2014, Rev. B vii

8 EC authorized representative Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX UK Telephone: Fax: Contact: Quality manager viii October 13, 2014, Rev. B

9 Table of Contents Copyright notice... iii Trademarks... iii Customer comments... iii Contacting Waters... iv Safety considerations... iv Considerations specific to the p-ism... v FCC radiation emissions notice... v Canada spectrum management emissions notice... v Electrical power safety notice... v Safety hazard symbol notice... v Equipment misuse notice... v Safety advisories... vi Operating this device... vi Applicable symbols... vi Audience and purpose... vii Intended use of the isocratic solvent manager... vii Industrial scientific and medical classification... vii Industrial scientific and medical classification: Industrial Scientific and Medical Group 1 Class B... vii October 13, 2014, Rev. B ix

10 EC authorized representative... vii 1 ACQUITY Advanced Polymer Chromatography Isocratic Solvent Manager Advanced Polymer Chromatography Location of the p-ism in a system Major components Flow path through the solvent management system Preparing for Operation Stacking system modules Installation recommendations for fittings Installing the leak sensor Resolving leak sensor errors Installing the waste and degasser vent tubing Priming the seal-wash system Priming the p-ism Priming a dry p-ism via the console Priming a dry p-ism using a syringe Monitoring LEDs Power LED Status LED p-ism control panel Maintenance Procedures Maintaining the p-ism Contacting Waters technical service x October 13, 2014, Rev. B

11 Maintenance schedule Spare parts Safety and handling Replacing solvent filters Cleaning the air filter in the door Replacing the air filter in the door Replacing the leak sensor Replacing the 22-µL filter Replacing the low-pressure inlet filters on the i 2 Valve inlet Replacing the vent-valve cartridge Replacing the i 2 Valve actuator Replacing the i 2 Valve cartridge Replacing the accumulator check valve Removing the primary head and replacing its seals Removing the accumulator head and replacing its seals Cleaning the instrument s exterior A Safety Advisories Warning symbols Specific warnings Caution advisory Warnings that apply to all Waters instruments and devices Electrical and handling symbols Electrical symbols Handling symbols October 13, 2014, Rev. B xi

12 B Specifications Physical specifications Environmental specifications Electrical specifications Input/output specifications Performance specifications Wetted materials of construction xii October 13, 2014, Rev. B

13 1 ACQUITY Advanced Polymer Chromatography Isocratic Solvent Manager Waters designed the ACQUITY Advanced Polymer Chromatography (APC) Isocratic Solvent Manager (p-ism) to be used for isocratic solvent delivery in polymer characterization applications. The p-ism, a single pump, operates at a maximum pressure of 103,421 kpa (1034 bar, 15,000 psi), and flow rates as high as 1 ml/min. The pressure decreases linearly, to 62,053 kpa (621 bar, 9000 psi), at flow rates up to 2 ml/min. The p-ism also hosts a number of features such as integrated seal wash, in-line degassing and the Intelligent Intake Valves (i 2 V), offer improved usability and ruggedness. Contents: Topic Page Advanced Polymer Chromatography Location of the p-ism in a system Major components Flow path through the solvent management system Advanced Polymer Chromatography Advanced Polymer Chromatography is an application technique that separates polymers in solution according to their size. The technique requires a low-dispersion ACQUITY system fitted with columns packed with rigid, sub-3-μm, high-pore-volume hybrid particles. The columns provide a separation medium that is robust and resists swelling, eliminating the problems associated with traditional, soft-gel polymeric columns. APC also accelerates the rate of analysis and improves chromatographic resolution, particularly for low-molecular-weight polymers and oligomers. October 13, 2014, Rev. B 13

14 1 ACQUITY Advanced Polymer Chromatography Isocratic Solvent Manager The modules that compose the ACQUITY APC system includes the p-ism, a sample manager with flow-through needle (SM-pFTN), a single zone column manager (CM-S), and a low-dispersion ACQUITY detector. All of these modules are designed for use with the aggressive solvents typically used for polymer separations. 14 October 13, 2014, Rev. B

15 Location of the p-ism in a system Location of the p-ism in a system The following diagram shows the location of the p-ism in the ACQUITY APC system: Bottle tray Detector CM-S SM-pFTN p-ism October 13, 2014, Rev. B 15

16 1 ACQUITY Advanced Polymer Chromatography Isocratic Solvent Manager Major components The following diagram shows the major components of the p-ism: On/off Power switch LED Run LED Location of pressure transducer cable connector (2) Accumulator check valve Location of sample manager purge solvent degasser Accumulator pump Vent valve 22-μL filter Primary pump i 2 Valve Seal wash pump Seal-wash waste tube Location of degasser vent tube Drip tray Low-pressure inlet filter Mobile phase degasser chamber Location of leak sensor cable connector Solvent vent tubing Leak sensor reservoir Drain fitting (to waste) p-ism components: Component 22-μL filter Accumulator pump Accumulator check valve Description Filters the solvent before it reaches the pump outlet. Receives solvent from the primary pump and delivers it to the system. A ball check valve that allows flow in only one direction. 16 October 13, 2014, Rev. B

17 Major components p-ism components: (Continued) Component Degasser vent tube Drip tray Drip tray drain fitting i 2 Valve Leak sensor Low-pressure inlet filter Mobile phase degasser chamber On/off switch Power LED Pressure transducer cable connectors Primary pump Run LED Sample manager purge solvent degasser Seal-wash pump Seal-wash waste tube Description Vents exhaust from the degasser pump. Collects and routes fluid leaks. Conducts leakage from the drip tray and routes it to a waste vessel. An electronically controlled ball check valve that allows flow in only one direction. Continuously monitors the p-ism for leaks and stops the system flow when its optical sensor detects approximately 1.5 ml of accumulated, leaked liquid in its surrounding reservoir. Note: The leak sensor is part of the system kit and should be installed and configured in the software. A filter that removes particulates from the solvent. Removes dissolved gasses from mobile phase solvent and exhausts them, and any condensates, through waste tubing. Note: Vacuum degassing can change the composition of mixed solvents. Powers the p-ism on or off. Indicates when the p-ism is powered on or off. The electrical connections for the pressure transducers located on the front of the actuators. Draws solvent, transferring it to the accumulator pump and system as part of the serial flow design. Indicates the p-ism status. Degasses the purge solvent which then flows through the sample manager. Note: Vacuum degassing can change the composition of mixed solvents. The pump that circulates solvent, to keep the actuators high-pressure seals free of contaminants. Conducts seal-wash waste to a waste vessel. October 13, 2014, Rev. B 17

18 1 ACQUITY Advanced Polymer Chromatography Isocratic Solvent Manager p-ism components: (Continued) Component Solvent vent tubing Vent valve Description Tubing that vents solvent to waste during priming. A valve that automatically switches to waste during priming and a block position during the leak test. Flow path through the solvent management system Flow through the p-ism: Solvent flows through the p-ism as follows: 1. The in-line vacuum degasser degasses the solvent. 2. The degassed solvent is filtered before entering the i 2 Valve. 3. The filtered solvent flows through the i 2 Valve (a check valve) and into the primary piston chamber. 18 October 13, 2014, Rev. B

19 Flow path through the solvent management system 4. The primary piston delivers solvent to the accumulator, the vent valve, and the in-line filter during transfer. 5. The accumulator piston delivers solvent, under pressure, to the vent valve and the in-line filter. 6. From the in-line filter, the solvent flows to the sample management system. October 13, 2014, Rev. B 19

20 1 ACQUITY Advanced Polymer Chromatography Isocratic Solvent Manager 20 October 13, 2014, Rev. B

21 2 Preparing for Operation Preparing the p-ism for operation involves installing the leak sensors, plumbing the waste tubing, priming the device and monitoring system status. Contents: Topic Page Stacking system modules Installation recommendations for fittings Installing the leak sensor Resolving leak sensor errors Installing the waste and degasser vent tubing Priming the seal-wash system Priming the p-ism Monitoring LEDs p-ism control panel Warning: Observe Good Laboratory Practices when you handle solvents. For reference, see the Material Safety Data Sheets for the solvents you use. Requirement: To maintain the efficiency of the p-ism and to obtain accurate, reproducible chromatograms, use only MS-grade solvents, water, and additives. For details, see the Solvent Considerations information and Controlling Contamination in Ultra Performance LC/MS and HPLC/MS Systems on the ACQUITY Advanced Polymer Chromatography System CD. October 13, 2014, Rev. B 21

22 2 Preparing for Operation Caution: To avoid damaging components of the p-ism do not pressurize solvent reservoirs in excess of 34.5 kpa (0.34 bar, 5psi). Recommendation: To ensure the solvent manager s optimal performance, elevate the solvent bottles above the pump inlet and vent properly. Stacking system modules Warning: To avoid spinal and muscular injury, do not attempt to lift the p-ism without assistance. To stack the modules: 1. Place the p-ism atop the system bench. 2. Place the rear feet of the next module in the system stack atop the p-ism and slide the added module backward until its rear alignment pin rests in the rear alignment slot on the p-ism. Alignment pin (2) Alignment slot (2) 3. Lower the front of the added module so that its front alignment pin rests in the front alignment slot on the p-ism. Repeat step 2 and step 3 for the remaining system modules. 22 October 13, 2014, Rev. B

23 Installation recommendations for fittings Installation recommendations for fittings Warning: To avoid personal contamination with biohazards, compounds that are toxic or corrosive, or the residues of such compounds, wear clean, chemical-resistant, powder-free gloves when reinstalling fittings. The system uses gold-plated compression screws and two-piece ferrules. See the diagram below for assembly orientation: Stainless steel ferrule with locking ring Stainless steel, gold -plated compression fitting Tubing Recommendations: To prevent bandspreading, ensure the tubing is fully bottomed in the fitting hole before tightening the compression fitting. For easier accessibility, use long compression screws to attach tubes to the injector and vent valve. Perform the solvent manager leak test whenever you replace or loosen fittings during maintenance (see the ACQUITY APC online Help). Whenever you loosen fittings during maintenance, examine for cracks, stripped threads, and deformations. Do not re-use stainless steel fittings more than six times. Required material Gloves: clean, powder-free, chemical-resistant When tightening system fittings, consult the following table. October 13, 2014, Rev. B 23

24 2 Preparing for Operation Installation recommendations for ACQUITY APC p-ism fittings: First use or reinstalled Fitting Long 1/4-28 fitting with flangeless ferrule and stainless steel lock ring, installed on 1/8-inch outside diameter (OD) tubing. End of lock ring with smaller inside diameter (ID) TP03377 Recommended tightening Finger-tight First use or reinstalled Lock ring Ferrule Long 1/4-28 fitting with flangeless ferrule and stainless steel lock ring, installed on.062-inch OD tubing. End of lock ring with smaller inside diameter (ID) Lock ring Ferrule Finger-tight First use Stainless steel (gold-plated) fitting with long flats and 2-piece stainless steel ferrule. TP03378 Finger-tight, plus 3/4-turn using wrench 3/4-turn Long flats 2-piece ferrule 24 October 13, 2014, Rev. B

25 Installation recommendations for fittings Installation recommendations for ACQUITY APC p-ism fittings: (Continued) Reinstalled Fitting Stainless steel (gold-plated) fitting with long flats and 2-piece stainless steel ferrule. TP02713 Recommended tightening Finger-tight, plus up to 1/6-turn using wrench 1/6-turn Long flats 2-piece ferrule First use Stainless steel (gold-plated) fitting with short flats and 2-piece stainless steel ferrule. Finger-tight, plus 3/4-turn using wrench 3/4-turn TP03375 Reinstalled Short flats 2-piece ferrule Stainless steel (gold-plated) fitting with short flats and 2-piece stainless steel ferrule. TP03374 Finger-tight, plus up to 1/6-turn using wrench 1/6-turn Short flats 2-piece ferrule October 13, 2014, Rev. B 25

26 2 Preparing for Operation Installation recommendations for ACQUITY APC p-ism fittings: (Continued) Fitting Recommended tightening First use and Reinstalled First use and Reinstalled First use and Reinstalled Long fitting with flangeless ferrule installed on 1/16-inch outside diameter (OD) tubing fitting Ferrule 5/16-inch compression fitting, ETFE ferrule with stainless steel frit and ferrule locking ring. ETFE ferrule with stainless steel frit 5/16-inch fitting Lock ring Knurled adapter fitting with ferrule. Ferrule Knurled adapter fitting Finger-tight Finger-tight Finger-tight Installing the leak sensor The p-ism can employ an optional leak sensor for detecting and collecting leakage. 26 October 13, 2014, Rev. B

27 Installing the leak sensor Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Warning: The leak sensor can be contaminated with biohazardous and/or toxic materials. Always wear clean, chemical-resistant, powder-free gloves when performing this procedure. Caution: To avoid damaging electrical parts, never disconnect an electrical assembly while power is applied to an instrument. To completely interrupt power, set the power switch to Off, and then unplug the power cord from the AC source. Wait 10 seconds thereafter before you disconnect an assembly. Required materials Gloves: clean, powder-free, chemical-resistant Leak sensor To install the leak sensor: 1. Power-off the p-ism. 2. Open the door of the p-ism, gently pulling its right-hand edge toward you. 3. Carefully unpack the new leak sensor. October 13, 2014, Rev. B 27

28 2 Preparing for Operation 4. Align the leak sensor s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place. T-bar Slot in leak sensor reservoir 5. Plug the leak sensor connector into the front of the instrument. 6. Power-on the p-ism. 7. In the Instrument console, select Isocratic Solvent Manager from the system tree. 8. In the ISM information window, click Control > Reset ISM, to reset the p-ism. 9. In the console, enable the leak sensor, to activate the leak sensor s leak detection capability. Resolving leak sensor errors After approximately 1.5 ml of liquid accumulates in the leak sensor reservoir, an alarm sounds, indicating that the leak sensor detected a leak. 28 October 13, 2014, Rev. B

29 Resolving leak sensor errors Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Warning: To avoid personal contamination with biohazards, compounds that are toxic or corrosive, or the residues of such compounds, wear clean, chemical-resistant, powder-free gloves when reinstalling fittings. Caution: To avoid scratching or otherwise damaging the leak sensor, do not allow buffered solvents to accumulate and dry on it; do not submerge it in a cleaning bath. Required materials Cotton swabs Gloves: clean, powder-free, chemical-resistant Nonabrasive, lint-free wipes To resolve a leak sensor error: 1. In the Instrument console s Leak Sensors dialog box, confirm that the p-ism s leak sensor detected a leak. Tip: If a leak is detected, a Leak Detected error message appears. October 13, 2014, Rev. B 29

30 2 Preparing for Operation Caution: To avoid damaging electrical components or circuitry of a system module, do not disconnect an electrical assembly from the module while the module remains connected to the ac supply source. Follow this procedure to completely interrupt power to the module: 1. Set the module s power switch to Off. 2. Disconnect the module s electrical supply cord from the ac source. Afterward, wait 10 seconds thereafter before you disconnect an assembly. 2. Power-off the p-ism. 3. Open the p-ism s door, gently pulling its right-hand edge toward you. 4. Locate the source of the leak, and make the repairs necessary to stop the leak. Caution: To avoid damaging the leak sensor, do not grasp it by the ribbon cable. 5. Remove the leak sensor from its reservoir, grasping it by its serrations, and pull upward on it. Tip: If you cannot easily manipulate the leak sensor after removing it from its reservoir, detach the connector from the front of the instrument. Serrations 30 October 13, 2014, Rev. B

31 Resolving leak sensor errors 6. Use a nonabrasive, lint-free wipe to dry the leak sensor p-ism. Prism Lint-free wipe TP Roll up a nonabrasive, lint-free wipe, and use it to absorb the liquid from the leak sensor reservoir and its surrounding area. Leak sensor reservoir Rolled up lint-free wipe 8. With a cotton swab, absorb any remaining liquid from the corners of the leak sensor reservoir and its surrounding area. Leak sensor reservoir Cotton swab October 13, 2014, Rev. B 31

32 2 Preparing for Operation 9. Align the leak sensor s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place. T-bar Slot in leak sensor reservoir 10. If you detached the connector from the front of the instrument, reattach it. 11. Power-on the p-ism. 12. In the Instrument console, select Isocratic Solvent Manager from the system tree. 13. In the ISM information window, click Control > Reset p-ism, to reset the p-ism. Installing the waste and degasser vent tubing To install the waste and degasser vent tubing: Caution: To prevent contamination, wear clean, chemical-resistant, powder-free gloves when installing or removing the waste and degasser vent tubing. 1. Wet the barbed drain fitting located at the bottom of the p-ism with methanol. 32 October 13, 2014, Rev. B

33 Installing the waste and degasser vent tubing Caution: To avoid distorting the drip tray or causing the drain cup to leak, restrain the drain cup when attaching or removing the waste tubing. 2. Hold the back of the drain cup, and then slide the waste tubing over the barbed drain fitting and route it to a suitable waste container. Drip tray Barbed drain fitting Drain cup Warning: To avoid releasing solvent vapors into the room, route the degasser vent tubing to a fume hood or other suitable exhaust system, or to a suitable waste container, ensuring the tubing's discharge end is at all times above the fluid level. Warning: To avoid spills, empty the waste container at regular intervals. Caution: To avoid fluid backup, you must ensure proper drainage of waste: Place the waste container below the system stack. Ensure that the waste and degasser vent tubes do not crimp or bend. A crimp or bend can impede flow to the waste container. Ensure the exit of the waste and degasser vent tubes is not immersed in waste solvent. If necessary, shorten each tube so that no portion of it drops below the top of the waste container (see next figure). 3. Route the degasser vent tubing to a suitable waste container. October 13, 2014, Rev. B 33

34 2 Preparing for Operation Representation of a suitable waste container: Priming the seal-wash system Priming the seal wash system lubricates the ISM s plungers, fills its tubing paths with solvent, and flushes away solvent or any precipitated salts that have been dragged past the plunger seals from the high-pressure side of the piston chambers. Prime the plunger seal wash on these occasions: After using buffered mobile phase When the p-ism has been inactive for a few hours or longer When the p-ism is dry 34 October 13, 2014, Rev. B

35 Priming the seal-wash system Caution: To avoid damaging the solenoid valve seats and seals in the solvent path, do not use a nonvolatile buffer as the seal-wash solvent. To avoid clogging system tubing, ensure the seal-wash solvent is 100% compatible with the mobile phase conditions. To avoid spreading contamination, do not recycle seal wash. Ensure that seal-wash contains at least 10% organic solvent, a concentration that prevents microbial growth and ensures that the seal wash can solubilize the mobile phase. Mobile phase and seal-wash compatibility: Mobile phase Buffer Seal Wash Aqueous Yes/No Water with at least 10% organic and is fully miscible with the mobile phase. Non-aqueous Yes/No Mobile phase without buffer or 100% IPA if miscible with mobile phase. Aqueous/non-aqueous Yes Mobile phase without buffer. mixture Aqueous/non-aqueous mixture No Mobile phase. Before priming the plunger seals, ensure the volume of seal-wash is adequate for priming. See also: Controlling Contamination in Ultra Performance LC/MS and HPLC/MS Systems on the ACQUITY Advanced Polymer Chromatography System CD. Required materials Gloves: clean, powder-free, chemical-resistant Seal wash solution 10-mL syringe (startup kit) Tubing adapter (startup kit) October 13, 2014, Rev. B 35

36 2 Preparing for Operation Compression fitting and ferrule long 1/4-28 fitting (startup kit) Tip: The seal-wash routine is self-priming. Nevertheless, using a syringe to draw seal wash through the system hastens the process. To prime the seal-wash system: 1. Ensure the seal-wash inlet tubing is immersed in the wash solvent. 2. Locate the appropriate seal-wash waste Teflon tubing. 3. Push the syringe plunger fully into the syringe barrel. 4. Connect the tubing adapter to the syringe. 5. Connect the compression fitting and ferrule to the end of the Teflon tubing in step Connect the Teflon tubing assembly from step 5 to the syringe assembly from step In the Instrument console, from the system tree, select Isocratic Solvent Manager. 8. Click Control > Prime seal wash, and then click Yes, to begin the seal wash priming process. 9. Slowly draw back on the syringe plunger, to draw seal wash solvent through the system. 10. When the seal wash begins to flow into the syringe showing relatively few air bubbles, click Control > Prime seal wash, to stop the priming process. 11. Disconnect the tubing from the syringe assembly, and connect it to the solvent waste bucket. Priming the p-ism You must prime the p-ism when it or the system that it is part of has never been used. You must also prime it when you restart a system, after the system has been idle for more then four hours, or when you change reservoirs or solvents. During priming, the vent valve moves to the vent position, ensuring minimal backpressure and directing the flow to waste. The flow rate during priming is 4 ml/min. 36 October 13, 2014, Rev. B

37 Priming the p-ism Caution: To prevent salts from precipitating in the system, introduce an intermediate solvent, such as water, when changing from buffers to high-organic-content solvents. Be sure to consult the solvent miscibility tables in the Solvent Considerations section. Ensure the solvent reservoirs contain sufficient solvent for adequate priming and that the waste container can hold all the used solvent. For example, at 4 ml/min, priming for 5 minutes uses about 20 ml of solvent. Warning: To avoid spills, empty the waste container at regular intervals. Requirement: In order for the degasser to function properly, the solvent line must be primed with solvent. Priming a dry p-ism via the console To prime the p-ism: 1. Open the instrument s front door. 2. Locate the appropriate solvent vent tubing. 3. In the Instrument console, select Isocratic Solvent Manager from the system tree. 4. In the ISM information window, click Control > Prime solvents. 5. In the Prime Solvents dialog box, select ISM solvent. 6. In the Time box, specify the number of minutes from 0.1 through Default: 2.0 minutes Recommendation: Prime the p-ism until a steady flow exits the vent tubing (typically 7 to 10 minutes or until no air bubbles are visible in the waste line). 7. Click Start. When solvent flows out of the vent tubing without bubbles, the path is primed. Requirement: There must be solvent in all tubes for the degasser to function correctly. October 13, 2014, Rev. B 37

38 2 Preparing for Operation Priming a dry p-ism using a syringe Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Caution: To prevent contamination, wear clean, chemical-resistant, powder-free gloves when priming a dry p-ism using a syringe. Required materials Gloves: clean, powder-free, chemical-resistant 10-mL syringe (startup kit) Tubing adapter (startup kit) Compression fitting and ferrule long 1/4-28 fitting (startup kit) To prime a dry p-ism: 1. Open the instrument s front door. 2. Follow the stainless steel solvent vent tubing from port 4 on the vent valve to the end of the connecting Teflon tube connected to the waste container. Vent valve Solvent vent tubing 3. Push the syringe plunger fully into the syringe barrel. 38 October 13, 2014, Rev. B

39 Monitoring LEDs 4. Connect the tubing adapter to the syringe. 5. Connect the compression fitting and ferrule to the end of the Teflon tubing in step Connect the Teflon tubing assembly from step 5 to the syringe assembly from step In the Instrument console, select Isocratic Solvent Manager from the system tree. 8. In the ISM information window, click Control > Prime solvents. 9. In the Prime Solvents dialog box, select ISM solvent. 10. In the Time box, specify the number of minutes from 0.1 through Recommendation: The default setting is 2.0 minutes. However, prime the p-ism until a steady flow exits the vent tubing (typically 3 minutes). 11. Click Start. 12. Slowly withdraw the syringe plunger, to pull solvent through the solvent path. When solvent flows out the vent tubing without bubbles, the path is primed. 13. Remove the syringe from the vent tubing, and connect the vent tubing to the waste container. Requirement: There must be solvent in all tubes for the degasser to function correctly. Monitoring LEDs Light emitting diodes on the p-ism indicate its state of functioning. Power LED The power LED, on the left-hand side of the p-ism s front panel, indicates when the p-ism is powered-on or powered-off. Power LED indications: LED mode and color Green Description Power is applied to the p-ism. October 13, 2014, Rev. B 39

40 2 Preparing for Operation Power LED indications: (Continued) LED mode and color Black (unlit) Description Power is not applied to the p-ism. Status LED The flow LED, on the right-hand side of the power LED on the p-ism s front panel, indicates the flow status. Flow LED indications: LED mode and color Unlit Steady green Flashing green Flashing red Steady red Description p-ism is idle. Solvent is flowing. Initializing and preparing for normal operation. Error state. Refer to the instrument console for information about the error. Indicates a failure of the p-ism that prevents its further operation. Power-off the module, and then restart it. If the LED is still steady red, contact your Waters service representative. p-ism control panel The control panel of the p-ism displays flow status, system pressure and total flow rate. Rule: You can edit these settings when the system is idle by clicking on the underlined value. You cannot edit p-ism settings while the system is running samples. 40 October 13, 2014, Rev. B

41 p-ism control panel p-ism control panel: Location of status System pressure Flow LED Flow rate Stop flow The following table describes the items in the p-ism control panel. p-ism control panel items: Control panel item Flow LED Status System pressure Flow rate (Stop flow) Description Displays on the front panel of the solvent manager the status of the flow state, unless communications are lost. Displays the status of the current operation. Displays system pressure, in kpa, bar, or psi. You can customize pressure units via the console. Displays the flow rate of solvent through all lines of the p-ism, from to ml/min, under normal operation, and to ml/min, when priming. Immediately stops all flow from the p-ism. You can access these additional functions by right-clicking anywhere in the p-ism control panel. Additional functions in the p-ism control panel: Control panel function Start up system Description Brings the system to operational conditions after an extended idle period or when switching to different solvents. October 13, 2014, Rev. B 41

42 2 Preparing for Operation Additional functions in the p-ism control panel: (Continued) Control panel function Prime solvent Prime seal wash Wash plungers Launch console Reset ISM Help Description Displays the Prime Solvent dialog box and allows for manual changeover or refreshing of solvent. Solvents are automatically shunted to waste. Starts priming the seal wash which lubricates the plungers, fills the tubing paths with solvent and flushes away solvent and or any precipitated salts that have been dragged past the plunger seals from the high-pressure side of the piston chambers. Initiates the plunger-wash sequence, which fills and then slowly empties the primary and accumulator chambers (with the current solvent composition) while performing a high speed/volume seal wash. This action helps prevent precipitate buildup on the pump plungers. Such a buildup can damage the high pressure seals. Launches the console. Resets the p-ism after an error condition. Displays the online Help for the console software. 42 October 13, 2014, Rev. B

43 3 Maintenance Procedures Keep to a maintenance schedule, and perform maintenance as required and described in this chapter. Contents: Topic Page Maintaining the p-ism Maintenance schedule Spare parts Safety and handling Replacing solvent filters Cleaning the air filter in the door Replacing the air filter in the door Replacing the leak sensor Replacing the 22-μL filter Replacing the low-pressure inlet filters on the i 2 Valve inlet Replacing the vent-valve cartridge Replacing the i 2 Valve actuator Replacing the i 2 Valve cartridge Replacing the accumulator check valve Removing the primary head and replacing its seals Removing the accumulator head and replacing its seals Cleaning the instrument s exterior Maintaining the p-ism Perform the procedures in this section when you discover that a component of the solvent manager malfunctioned or during routine maintenance. For October 13, 2014, Rev. B 43

44 3 Maintenance Procedures information about isolating such problems, consult the Instrument console online Help. Contacting Waters technical service If you are located in the USA or Canada, report malfunctions or other problems to Waters Technical Service ( ). From elsewhere, phone the Waters corporate headquarters in Milford, Massachusetts (USA), or contact your local Waters subsidiary. The Waters web site includes phone numbers and addresses for Waters locations worldwide. Visit When you contact Waters, be prepared to provide this information: Error message (if any) Nature of the symptom Serial number of the system module and its firmware version, if applicable Flow rate Operating pressure Solvent(s) Detector settings (sensitivity and wavelength) Type and serial number of column(s) Sample type and diluent Data software version and serial number ACQUITY APC system workstation model and operating system version For complete information on reporting shipping damages and submitting claims, see the publication Waters Licenses, Warranties, and Support Services. Locating system serial numbers The serial number on the system s instruments and devices facilitates service and support. Serial numbers also provide a way to create single log entries for each module, so that you can review the usage history of a particular module. Be prepared to provide the serial numbers of the instruments or devices in your system when you contact Waters customer support. 44 October 13, 2014, Rev. B

45 Maintenance schedule To view the information for a system module: 1. In the Instrument console, select a module from the system tree. 2. Click Configure > View module information. Result: The Module Information dialog box displays this information: Serial number Firmware version Firmware checksum Component software version Alternatives: From the main window, place the pointer over the visual representation of the system module for which you want to view information. Obtain the serial number from the printed labels affixed to the rear panels of system modules or inside their front doors. Maintenance schedule Perform the following routine maintenance on the p-ism to ensure reliable operation and accurate results. When using the system throughout the day (and on nights and weekends), or when using aggressive solvents such as buffers, perform these maintenance tasks more frequently. Recommended routine maintenance schedule: Maintenance procedure Replace solvent filters Clean the air filter in the door Replace the air filter in the door Required frequency During scheduled routine maintenance or as needed For information... See page 48. As needed See page 49. During scheduled routine maintenance or as needed See page 49. Replace the leak sensor As needed See page 50. October 13, 2014, Rev. B 45

46 3 Maintenance Procedures Recommended routine maintenance schedule: (Continued) Maintenance procedure Required frequency For information... Replace the 22-μL filter Replace the low-pressure inlet filters on the i 2 Valve Replace the vent-valve cartridge Replace the i 2 Valve actuator Replace the i 2 Valve cartridge Replace the accumulator check valve Replace the head seals Clean the instrument with a soft, lint-free cloth, or paper dampened with water During scheduled routine maintenance or as needed During scheduled routine maintenance or as needed See page 53. See page 54. As needed See page 56. During scheduled routine maintenance or as needed During scheduled routine maintenance or as needed During scheduled routine maintenance or as needed During scheduled routine maintenance or as needed See page 59. See page 68. See page 76. See page 78 and page 96. As needed See page 110. Spare parts To ensure that your system operates as designed, use only Waters Quality Parts. Visit for information about Waters Quality Parts, including how to order them. 46 October 13, 2014, Rev. B

47 Safety and handling Safety and handling Bear in mind the following safety considerations when performing maintenance procedures: Warning: To avoid personal contamination with biohazards, compounds that are toxic or corrosive, or the residues of such compounds, wear clean, chemical-resistant, powder-free gloves when reinstalling fittings. Warning: To prevent injury, always observe Good Laboratory Practice when handling solvents, changing tubing, or operating the instrument. Know the physical and chemical properties of the solvents used (see the Material Safety Data Sheets for the solvents in use). Warning: To avoid electric shock, do not remove protective panels from the sample manager. The panels cover components serviceable only by Waters technicians. Caution: To avoid damaging electrical components or circuitry of a system module, do not disconnect an electrical assembly from the module while the module remains connected to the ac supply source. Follow this procedure to completely interrupt power to the module: 1. Set the module s power switch to Off. 2. Disconnect the module s electrical supply cord from the ac source. Afterward, wait 10 seconds thereafter before you disconnect an assembly. October 13, 2014, Rev. B 47

48 3 Maintenance Procedures Replacing solvent filters Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Caution: Wear clean, chemical-resistant, powder-free gloves when handling the solvent filter. Oil from your hands can contaminate the solvent filter. Required materials Gloves: clean, powder-free, chemical-resistant New solvent filter To replace a solvent filter: 1. Remove the filter end of the solvent tubing from the solvent bottle. 2. Remove the old solvent filter from the short piece of PTFE tubing. 3. Insert the new solvent filter into the PTFE tubing, pushing until it contacts the solvent tubing. Solvent filter Solvent tubing PTFE tubing 4. Insert the filter end of the solvent tubing into the solvent bottle. 5. Shake the filter, to remove any air from it. 6. Prime the p-ism (see page 36). 48 October 13, 2014, Rev. B

49 Cleaning the air filter in the door Cleaning the air filter in the door Required materials Mild detergent and water To clean the air filter: 1. Lift the air filter, removing it from the frame. Screw (4) Air filter frame 2. Using a mild detergent and water clean the filter and then dry it. 3. Align the cleaned air filter within the air filter frame. Replacing the air filter in the door If you cannot clean the air filter by washing, replace it with a new filter. Required materials p-ism air filter October 13, 2014, Rev. B 49

50 3 Maintenance Procedures To replace the air filter: 1. Lift the air filter up, removing it from the frame. Screw (4) Air filter frame 2. Remove the old air filter from the air filter frame, and discard it. 3. Align the new air filter within the air filter frame. Replacing the leak sensor Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Warning: To avoid personal contamination with biohazards, compounds that are toxic or corrosive, or the residues that make up such compounds, wear clean, chemical-resistant, powder-free gloves when handling the leak sensor. Required materials Gloves: clean, powder-free, chemical-resistant Leak sensor 50 October 13, 2014, Rev. B

51 Replacing the leak sensor To replace the leak sensor: Caution: To avoid damaging electrical components or circuitry of a system module, do not disconnect an electrical assembly from the module while the module remains connected to the ac supply source. Follow this procedure to completely interrupt power to the module: 1. Set the module s power switch to Off. 2. Disconnect the module s electrical supply cord from the ac source. Afterward, wait 10 seconds thereafter before you disconnect an assembly. 1. Power-off the p-ism. 2. Open the p-ism s door, gently pulling its right-hand edge toward you. 3. Press down on the tab, to detach the leak sensor connector from the front of the instrument. Tab Leak sensor connector October 13, 2014, Rev. B 51

52 3 Maintenance Procedures 4. Remove the leak sensor from its reservoir, grasping it by its serrations, and pull upward on it. Serrations 5. Unpack the new leak sensor. 6. Align the leak sensor s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place. T-bar Slot in leak sensor reservoir 7. Connect the leak sensor connector to the front of the instrument. 8. Power-on the p-ism. 9. In the Instrument console, select Isocratic Solvent Manager from the system tree. 10. In the p-ism information window, click Control > Reset ISM, to reset the p-ism. 52 October 13, 2014, Rev. B

53 Replacing the 22-µL filter Replacing the 22-µL filter Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Caution: To avoid spreading contamination, wear clean, chemical-resistant, powder-free gloves when replacing the 22-μL mixer/filter. Required materials 1/4-inch, open-end wrench 5/8-inch, open-end wrench 22-μL mixer/filter Gloves: clean, powder-free, chemical-resistant To replace the 22-μL filter: 1. Flush the p-ism with non-hazardous solvent. 2. Stop the solvent flow. 3. Holding the 22-μL filter in place with the 5/8-inch, open-end wrench, disconnect the inlet compression fitting using the 1/4-inch, open-end wrench. Inlet compression fitting October 13, 2014, Rev. B 53

54 3 Maintenance Procedures 4. Holding the 22-μL filter with the 5/8-inch, open-end wrench, disconnect the outlet compression fittings using the 1/4-inch wrench. Location of outlet compression fitting 5. Remove the old 22-μL filter from the bracket. 6. Unpack the new 22-μL filter. 7. Insert the new 22-μL filter into the bracket. 8. Reattach the compression fittings to the 22-μL filter, and tighten them finger-tight plus an additional 1/6-turn, for existing fittings, or 3/4-turn for new fittings. Replacing the low-pressure inlet filters on the i 2 Valve inlet Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Caution: To avoid spreading contamination, wear clean, chemical-resistant, powder-free gloves when replacing the low-pressure inlet filters. Required materials Gloves: clean, powder-free, chemical-resistant Low-pressure inlet filter To replace the low-pressure inlet filters on the i 2 Valve inlet: 1. Power-off the p-ism. 54 October 13, 2014, Rev. B

55 Replacing the low-pressure inlet filters on the i 2 Valve inlet 2. Open the p-ism s door, gently pulling its right-hand edge toward you. 3. Unscrew the PEEK compression fitting attached to the knurled adapter fitting in the i 2 Valve inlet. Adapter fitting PEEK compression fitting 4. Remove the low-pressure inlet filter by pulling the PEEK compression fitting from the tube. PEEK compression fitting Tube 5. Replace the PEEK compression fitting over the end of the tube. 6. Slide the metal locking ring onto the tube, ensuring that the thicker end of the metal locking ring faces the compression fitting. Sliding metal locking ring on tubing: Metal locking ring Tube Compression fitting October 13, 2014, Rev. B 55

56 3 Maintenance Procedures 7. Place a new filter on the end of the tube. Filter Tube Compression fitting 8. Insert the filter end of tube into the knurled adapter, and finger-tighten the PEEK compression fitting. Adapter PEEK compression fitting 9. Power-on the p-ism and then prime it. Replacing the vent-valve cartridge Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Caution: To avoid spreading contamination, wear clean, chemical-resistant, powder-free gloves when replacing the vent-valve cartridge. Required materials 1/4-inch, open-end wrench Allen wrench, 2-mm (startup kit) Gloves: clean, powder-free, chemical-resistant 56 October 13, 2014, Rev. B

57 Replacing the vent-valve cartridge Vent-valve cartridge To replace the vent-valve cartridge: 1. In the Instrument console, from the system tree select ISM. 2. In the ISM information window, click Interactive Display. 3. In the ISM Interactive Display dialog box, click Control. 4. Ensure the vent valve is set to the Vent position. Tip: To change the setting to Vent, click the underlined vent-valve position, and select Vent. Interactive display showing vent-valve setting: Filter 5. Use the 1/4-inch wrench to remove the fittings attached to the vent-valve cartridge. Vent-valve cartridge: Hex screw in 10 o clock position Outlet to filter From accumulator Vent to waste October 13, 2014, Rev. B 57

58 3 Maintenance Procedures 6. Use the 2-mm Allen wrench to remove the hex screw at the 10 o clock position on the vent-valve cartridge. 7. Remove the vent-valve cartridge from the vent-valve assembly by pulling straight forward. 8. Unpack the replacement vent-valve cartridge. 9. Ensure that the groove in the cartridge housing aligns with the groove on the drive clamp. Tip: If the grooves fail to align, turn the drive clamp until they align. Note: Be careful not to scratch the drive clamp or body. Vent-valve cartridge housing Aligned grooves Drive clamp 10. Insert the new vent valve cartridge into the vent valve cartridge chamber. Requirements: Orient the new cartridge exactly as the old one was oriented. The vent-valve cartridge must slide fully into the vent-valve assembly. If it does not, contact Waters Technical Service. 11. Insert the 2-mm hex screw at the 10 o clock position on the vent-valve cartridge. 12. Use the 1/4-inch wrench to reattach all fittings, and tighten existing fittings 1/6-turn beyond finger-tight and new fittings 3/4-turn beyond finger-tight. 13. Prime the p-ism (see page 36). 58 October 13, 2014, Rev. B

59 Replacing the i 2 Valve actuator Replacing the i 2 Valve actuator Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Caution: To avoid damaging the i 2 Valve actuator, do not attempt to push or pull liquid or gas through the valve's inlet or outlet ports. To prevent contamination, wear clean, chemical-resistant, powder-free gloves when replacing the i 2 Valve actuator. Required materials T8 TORX driver 1/4-inch, open-end wrench 5/16-inch, open-end wrench Gloves: clean, powder-free, chemical-resistant i 2 Valve actuator i 2 Valve cartridge (recommended) To replace the i 2 Valve actuator: Caution: To avoid damaging electrical components or circuitry of a system module, do not disconnect an electrical assembly from the module while the module remains connected to the ac supply source. Follow this procedure to completely interrupt power to the module: 1. Set the module s power switch to Off. 2. Disconnect the module s electrical supply cord from the ac source. Afterward, wait 10 seconds thereafter before you disconnect an assembly. 1. Flush the p-ism with non-hazardous solvent. 2. Power-off the p-ism. October 13, 2014, Rev. B 59

60 3 Maintenance Procedures Tip: The p-ism is referred to as pump on the warning label affixed to the i 2 Valve actuator. Caution: To avoid damage to the connector or cable, grasp the i 2 Valve connector by the knurled diameter. 3. Grasp the i 2 Valve connector by the knurled diameter, and pull it toward you, disconnecting it from its receptacle. Knurled diameter Connector receptacle i 2 Valve connector 4. Remove the finger-tight low-pressure filter and inlet tubing from the knurled adapter fitting which is attached to the inlet of the i 2 Valve. Adapter fitting Finger-tight inlet-tubing fitting i 2 Valve 60 October 13, 2014, Rev. B

61 Replacing the i 2 Valve actuator 5. Use the 5/16-inch, open-end wrench to loosen the shell nut, and then fully unscrew it. Place 5/16-inch open-end wrench here Caution: When you remove the valve assembly, ensure the PEEK washer, which is normally on the top face of the i 2 Valve cartridge, does not remain in the head (see step 11). Never place the actuator assembly or electrical connector in the drip tray. October 13, 2014, Rev. B 61

62 3 Maintenance Procedures 6. Remove the i 2 Valve actuator from the bottom of the primary pump head. Bottom of primary pump head Knurled adapter fitting i 2 Valve actuator 7. Remove the knurled adapter fitting with ETFE ferrule from the old i 2 Valve actuator. 8. Use the T8 TORX driver to loosen 1/2-turn the 4 screws that secure the clamping plates. 9. Ensure the shell nut remains free to rotate and that the plates slide open. 62 October 13, 2014, Rev. B

63 Replacing the i 2 Valve actuator Tips: Avoid touching the clamping plate tabs when loosening the screws. You can rotate the shell nut to gain access to all 4 screws. Clamping plate tab (2) Shell nut Clamping plate (2) T8 TORX screw (4) 10. Remove the cartridge from the i 2 Valve actuator when both plates are in the maximum open position, ensuring the low-pressure gasket is also removed. Tip: If you cannot remove the cartridge from the valve actuator, rotate the cartridge 1/2-turn, and then remove it. 11. Ensure the PEEK washer is inserted into the cartridge so that its chamfered edge faces away from the cartridge. Chamfered edge facing away from cartridge PEEK washer i 2 Valve cartridge 12. Use the T8 TORX driver to loosen 1/2-turn the 4 screws that secure the clamping plates on the new i 2 Valve actuator. 13. Ensure the plates are loose and in their maximum open position. October 13, 2014, Rev. B 63

64 3 Maintenance Procedures Tips: Avoid touching the clamping plate tabs when loosening the screws. You can rotate the shell nut to gain access to all 4 screws. 14. Insert the cartridge you removed from the old i 2 Valve actuator into the new actuator, grooved end first. Recommendation: Replace the cartridge whenever you replace the i 2 Valve actuator. See step 11 on page 72. Grooved end of cartridge i 2 Valve actuator 15. With one hand, squeeze the 2 clamping plate tabs on the i 2 Valve actuator, to hold the clamping plates against the cartridge. 64 October 13, 2014, Rev. B

65 Replacing the i 2 Valve actuator Requirement: The clamping plates must be fully engaged in the cartridge groove. Clamping plate tab Clamping plate tab 16. While squeezing the clamping plate tabs, use the T8 TORX driver to tighten the 4 screws that secure the plates rotating the shell nut so you can access all of the screws. Clamping plate tab (2) Clamping plates Shell nut Clamping plate (2) T8 TORX screw (4) October 13, 2014, Rev. B 65

66 3 Maintenance Procedures 17. Tighten the screws in the sequence shown. Repeat the tightening sequence at least 3 times, gradually increasing torque until the screws are uniformly tight. Start here 18. Insert the knurled adapter fitting with the ETFE ferrule in the inlet port on the i 2 Valve assembly, and tighten it finger tight.orient the i 2 Valve assembly so that the cable exits from the left-hand side. Adapter fitting i 2 Valve 19. Insert the i 2 Valve assembly into the bottom of the primary pump head, and route the cable behind the valve actuator. 20. Finger-tighten the shell nut, to secure the valve. Tip: Expect to be able to rotate the shell nut approximately 5 full turns before it is finger-tight. 66 October 13, 2014, Rev. B

67 Replacing the i 2 Valve actuator 21. Use the 5/16-inch, open-end wrench to tighten the nut an additional 1/8-turn. Place 5/16-inch open-end wrench here 22. Reinstall the inlet tubing with the low-pressure filter into the knurled adapter fitting and finger-tighten it. Adapter fitting Inlet tubing Low pressure filter October 13, 2014, Rev. B 67

68 3 Maintenance Procedures 23. Align the white arrow on the i 2 Valve connector with the white arrow on the receptacle, in the 12 o clock position, and insert the connector into the receptacle. White arrows Connector receptacle i 2 Valve connector 24. Power-on the p-ism. 25. Prime the p-ism (see page 36). Replacing the i 2 Valve cartridge Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Caution: To avoid damaging the i 2 Valve actuator, do not attempt to push or pull liquid or gas through the valve's inlet or outlet ports. To prevent contamination, wear clean, chemical-resistant, powder-free gloves when replacing the i 2 Valve cartridge. Required materials T8 TORX driver 1/4-inch, open-end wrench 5/16-inch, open-end wrench Gloves: clean, powder-free, chemical-resistant 68 October 13, 2014, Rev. B

69 Replacing the i 2 Valve cartridge i 2 Valve cartridge To replace the i 2 Valve cartridge: Caution: To avoid damaging electrical components or circuitry of a system module, do not disconnect an electrical assembly from the module while the module remains connected to the ac supply source. Follow this procedure to completely interrupt power to the module: 1. Set the module s power switch to Off. 2. Disconnect the module s electrical supply cord from the ac source. Afterward, wait 10 seconds thereafter before you disconnect an assembly. 1. Flush the p-ism with non-hazardous solvent. 2. Power-off the p-ism. Tip: The p-ism is referred to as pump on the warning label affixed to the i 2 Valve actuator. 3. Grasp the i 2 Valve connector by the knurled diameter, and pull it toward you, disconnecting it from its receptacle. Knurled diameter Connector receptacle i 2 Valve connector October 13, 2014, Rev. B 69

70 3 Maintenance Procedures 4. Remove the finger-tight low-pressure filter and inlet tubing from the knurled adapter fitting which is attached to the inlet of the i 2 Valve. Adapter fitting Inlet tubing Low pressure filter 5. Use the 5/16-inch, open-end wrench to loosen the shell nut, and then fully unscrew it. Place 5/16-inch wrench here 70 October 13, 2014, Rev. B

71 Replacing the i 2 Valve cartridge Caution: When you remove the valve assembly, ensure the PEEK washer, which is normally on the top face of the i 2 Valve cartridge, does not remain in the head. See page 72. Never place the actuator assembly or electrical connector in the drip tray. 6. Remove the i 2 Valve actuator from the bottom of the primary pump head. 7. Use the T8 TORX driver to loosen 1/2-turn the 4 screws that secure the plates. 8. Ensure the shell remains free to rotate and that the plates slide open. Tips: Avoid touching the clamping plate tabs when loosening the screws. You can rotate the shell nut to gain access to all 4 screws. For ease of use, do not back the screws out fully. Caution: To avoid damaging the i 2 Valve actuator, do not back the screws out fully. Clamping plate tab (2) Shell nut Clamping plate (2) T8 TORX screw (4) 9. Remove the cartridge from the i 2 Valve actuator when both plates are in the maximum open position, ensuring that the low-pressure gasket is also removed (see step 11). Tip: If you cannot remove the cartridge from the valve actuator, rotate the cartridge 1/2-turn, and then remove it. October 13, 2014, Rev. B 71

72 3 Maintenance Procedures 10. Unpack the new cartridge. 11. Ensure the PEEK washer is inserted into the cartridge so that its chamfered edge faces away from the cartridge. Chamfered edge facing away from cartridge PEEK washer i 2 Valve cartridge 12. With the clamping plates still open, insert the cartridge into the i 2 Valve actuator, grooved end first. Grooved end of cartridge i 2 Valve actuator 13. With one hand, squeeze the 2 clamping plate tabs on the i 2 Valve actuator, to hold the clamping plates against the cartridge. 72 October 13, 2014, Rev. B

73 Replacing the i 2 Valve cartridge Requirement: The clamping plates must be fully engaged in the cartridge groove. Clamping plate tab Clamping plate tab 14. While squeezing the clamping plate tabs, use the TORX driver to tighten the 4 screws that secure the plates, following the torquing pattern shown in step 15. Tip: You can rotate the shell nut to gain access to all 4 screws. Clamping plate tab (2) Clamping plates Shell nut Clamping plate (2) T8 TORX screw (4) October 13, 2014, Rev. B 73

74 3 Maintenance Procedures 15. Follow the pattern shown below when tightening the screws that secure the plates, and repeat it at least 3 times, gradually increasing the torque until the screws are tight. Start here 16. Orient the i 2 Valve assembly so that the cable exits from the left-hand side. Insert the i 2 Valve assembly into the bottom of the primary pump head, and route the cable behind the valve actuator. 17. Finger-tighten the shell nut to secure the valve. Tip: Expect to be able to rotate the shell nut approximately 5 full turns before it is finger-tight condition. 18. Use the 5/16-inch, open-end wrench to tighten the nut an additional 1/8-turn. Place 5/16-inch open-end wrench here 74 October 13, 2014, Rev. B

75 Replacing the i 2 Valve cartridge 19. Reinstall the inlet tubing with the low-pressure filter into the knurled adapter and finger-tighten. Adapter fitting Inlet tubing Low pressure filter 20. Align the white arrow on the i 2 Valve connector with the white arrow on the receptacle, in the 12 o clock position, and insert the connector into the receptacle. White arrows Connector receptacle i 2 Valve connector 21. Power-on the p-ism. 22. Prime the p-ism (see page 36). October 13, 2014, Rev. B 75

76 3 Maintenance Procedures Replacing the accumulator check valve Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Caution: To prevent contamination, wear clean, chemical-resistant, powder-free gloves when replacing the check valve. Required materials 1/2-inch, open-end wrench 1/4-inch, open-end wrench 5/16-inch, open-end wrench Accumulator check valve assembly Gloves: clean, powder-free, chemical-resistant To replace the accumulator check valve: Caution: To avoid damaging electrical components or circuitry of a system module, do not disconnect an electrical assembly from the module while the module remains connected to the ac supply source. Follow this procedure to completely interrupt power to the module: 1. Set the module s power switch to Off. 2. Disconnect the module s electrical supply cord from the ac source. Afterward, wait 10 seconds thereafter before you disconnect an assembly. 1. Flush the p-ism with non-hazardous solvent. 2. Power-off the p-ism. Tip: Move the solvent bottles to a location below the p-ism. 76 October 13, 2014, Rev. B

77 Replacing the accumulator check valve 3. While holding the check valve in place with the 5/16-inch, open-end wrench, use the 1/4-inch, open-end wrench to disconnect the compression fitting. Accumulator check valve Place 5/16-inch wrench here Compression fitting Caution: When you remove the valve assembly, ensure the PEEK washer, which is normally on the top face of the check valve, does not remain in the head (see the figure after step 6). 4. Use the 1/2-inch, open-end wrench to loosen the check valve, and then remove the check valve assembly from the head. Accumulator check valve 5. Unpack the new check valve. October 13, 2014, Rev. B 77

78 3 Maintenance Procedures 6. Make sure the new PEEK washer is inserted into the new check valve so that its chamfered edge faces away from the check valve. Chamfered edge facing away from check valve PEEK washer Check valve 1/2-inch Hex nut 5/16-inch, open-end wrench flat 7. Insert the check valve assembly into the head, and use the 1/2-inch wrench to tighten the check valve nut 1/8-turn beyond finger-tight. 8. Use the 5/16-inch, open-end wrench to hold the check valve in place, and then reattach the compression fitting to the check valve. Tip: Use the 1/4-inch wrench to tighten the compression fitting 1/6-turn beyond finger-tight for existing stainless steel tubing assembly, or 3/4-turn beyond finger-tight for a new stainless steel tubing assembly. 9. Power-on the p-ism. 10. Prime the p-ism (see page 36). Removing the primary head and replacing its seals See the ACQUITY APC online Help to help determine whether you need to replace the primary head seals. 78 October 13, 2014, Rev. B

79 Removing the primary head and replacing its seals Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Caution: To prevent contamination, wear clean, chemical-resistant, powder-free gloves when removing and replacing the head seals. Required materials Compressed air Gloves: clean, powder-free, chemical-resistant Methanol Plunger (recommended) Plunger seal and plunger-seal spacer Fluoropolymer O-ring Seal-wash seal Required tools 1/4-inch, open-end wrench 5/16-inch, open-end wrench Plunger removal tool (recommended) Sharp tool T27 TORX driver (startup kit) Torque wrench To remove the primary head: 1. Flush the p-ism with nonhazardous solvent. 2. In the Instrument console, select Isocratic Solvent Manager from the system tree. 3. In the p-ism information window, click Maintain > Heads. 4. In the Head Maintenance dialog box, select the primary head. 5. Click Move Backward, and then wait for the plunger to stop. October 13, 2014, Rev. B 79

80 3 Maintenance Procedures Caution: To avoid damaging electrical components or circuitry of a system module, do not disconnect an electrical assembly from the module while the module remains connected to the ac supply source. Follow this procedure to completely interrupt power to the module: 1. Set the module s power switch to Off. 2. Disconnect the module s electrical supply cord from the ac source. Afterward, wait 10 seconds thereafter before you disconnect an assembly. 6. Power-off the p-ism. Tip: The p-ism is referred to as pump on the warning label affixed to the i 2 Valve actuator. Caution: To avoid damage to the connector or cable, grasp the i 2 Valve connector by the knurled diameter. 7. Move the solvent bottles to a location below the solvent manager. 8. Grasp the i 2 Valve connector by the knurled diameter, and pull it toward you, disconnecting it from its receptacle. Knurled diameter Connector receptacle i 2 Valve connector 80 October 13, 2014, Rev. B

81 Removing the primary head and replacing its seals 9. Loosen the 5/16-inch, compression fitting holding the low-pressure filter, and remove the filter from its knurled adapter fitting. Finger-tight inlet-tubing fitting i 2 Valve 10. Use the 5/16-inch, open-end wrench to loosen the shell nut, and then fully unscrew it. Place 5/16-inch open-end wrench here Caution: When you remove the valve assembly, ensure the PEEK washer, which is normally on the top face of the i 2 Valve cartridge, does not remain in the head (see page 72). Never place the actuator assembly or electrical connector in the drip tray. October 13, 2014, Rev. B 81

82 3 Maintenance Procedures 11. Remove the i 2 Valve actuator from the bottom of the primary pump head. Bottom of primary pump head i 2 Valve actuator 12. Remove the seal wash tubing secured to the seal wash housing by compression screws. Location of seal wash tubing 82 October 13, 2014, Rev. B

83 Removing the primary head and replacing its seals 13. Use the 1/4-inch open-end wrench to disconnect the outlet-tubing from the bottom of the transducer. Transducer Outlet-tubing 14. Disconnect the pressure transducer cable from the bulkhead by squeezing on the tabs and pulling gently. Pressure transducer cable connector Tabs October 13, 2014, Rev. B 83

84 3 Maintenance Procedures 15. Using the T27 TORX driver, loosen the 2 head bolts 1/2-turn. The bolts are accessible from the front of the pressure transducer. Head bolt (2) Caution: To avoid damaging the plunger, support the head from below as you remove it. 16. Using the T27 TORX driver, loosen and remove the 2 support plate bolts, and then gently pull the head and support plate off the actuator housing, making sure not to tilt the head during the extraction. Support plate bolt (2) 84 October 13, 2014, Rev. B

85 Removing the primary head and replacing its seals Caution: To avoid damaging the plunger, support the pump head from below as you remove it. Pulling head and support plate off actuator housing: Plunger Head and support plate October 13, 2014, Rev. B 85

86 3 Maintenance Procedures Warning: To avoid hand lacerations, use care when removing the old plunger. Bending the plunger shaft can cause it to break. 17. Use the recessed side of the plunger removal tool to apply pressure to both sides of the release collar, and then remove the old plunger. Recessed lip of plunger removal tool TP02990 Spring-loaded release collar Plunger Plunger removal tool TP Remove the plunger removal tool from the release collar. 86 October 13, 2014, Rev. B

87 Removing the primary head and replacing its seals To remove the primary plunger seals: 1. Stand the head upright on a clean surface. 2. Using the T27 TORX driver, completely loosen the 2 head bolts to release the support plate from the pump head. Requirement: If you remove the transducer and head bolts, be sure to reuse the head bolt washers when reassembling the pump head. Head bolt access hole 3. Lift the pump head from the support plate. Pump head Seal wash housing Support plate October 13, 2014, Rev. B 87

88 3 Maintenance Procedures 4. Lift the seal-wash housing from the support plate. Seal wash housing Support plate 5. Pull the seal-wash seal from the seal-wash housing. Seal wash spacer 6. Taking care not to scratch any surfaces, carefully withdraw the plunger seal and spacer. Plunger seal 88 October 13, 2014, Rev. B

89 Removing the primary head and replacing its seals Caution: To avoid scratching any metal surfaces, use care when using a sharp tool to remove the fluoropolymer O-ring. 7. Taking care not to scratch any surfaces, use a sharp tool to remove the fluoropolymer O-ring from the pump head. 8. Inspect the seal-wash housing surface and pump head surface, ensuring both are free from scratches and particulates. 9. Lubricate the new fluoropolymer O-ring with methanol, and press the O-ring into its seat with your thumbs. 10. Lubricate the new plunger seal spacer with methanol and place it in the seal-wash housing. Plunger seal Seal-wash housing 11. Spray the new plunger seal with compressed air to remove any particulates. October 13, 2014, Rev. B 89

90 3 Maintenance Procedures 12. Orient the seal-wash housing so that the holes on its side align with the holes on the side of the pump head, and then guide it into place. Seal-wash housing Pump head 13. Spray the seal-wash seal with compressed air to remove any particulates. 14. Lubricate the new seal-wash seal with methanol, place it in the seal-wash housing, and press it into place using the soft side of your thumb. Seal-wash seal 90 October 13, 2014, Rev. B

91 Removing the primary head and replacing its seals 15. Place the support plate on top of the pump head, ensuring the round side of the plate is oriented toward the bottom side of the head. Round side of support plate Bottom side of pump head 16. Holding the assembly together, use the T27 TORX driver, fully tighten the 2 head bolts until screw bottoms out then unscrew 1/4-turn. T27 TORX driver Head bolt access hole Head bolt access hole To reattach the primary head: 1. Flip the assembly over, and then lubricate the seals with methanol. October 13, 2014, Rev. B 91

92 3 Maintenance Procedures Seals 2. Carefully insert the sapphire plunger shaft into the pump head until the plunger shaft is no longer visible, ensuring the shaft does not contact the support plate. Recommendation: Replace the plunger whenever you replace the plunger seal. Plunger shaft Support plate Caution: To avoid damaging the plunger, ensure that the head assembly is not tilted relative to the actuator housing when you position it on the p-ism. 3. Carefully slide the head assembly and sapphire plunger into the actuator housing, making sure not to tilt the head. 92 October 13, 2014, Rev. B

93 Removing the primary head and replacing its seals 4. Hold the head assembly securely against the actuator housing, and then use the T27 TORX driver to tighten the support plate bolts securely. Pump head bolt (2) Support plate bolt (2) 5. Connect the pressure transducer cable to the bulkhead. 6. Power-on the p-ism. 7. In the Instrument console, select Isocratic Solvent Manager from the system tree. 8. In the ISM information window, click Maintain > Heads. 9. In the Head Maintenance dialog box, select the primary head. 10. Click Move Forward, and then wait for the piston to engage the plunger sphere. Caution: To avoid damaging the plunger, alternately tighten the the support plate screws 1/4-turn so that they are uniformly torqued. 11. Use the torque driver to tighten each pump head bolt to 40 inch-pounds of torque. 12. After tightening each pump head bolt to 40 inch-pounds of torque, use the torque driver to tighten each pump head bolt to 60 inch-pounds of torque. 13. After tightening each pump head bolt to 60 inch-pounds of torque, use the torque driver to tighten each pump head bolt to 80 inch-pounds of torque. October 13, 2014, Rev. B 93

94 3 Maintenance Procedures 14. After tightening each pump head bolt to 80 inch-pounds of torque, use the torque driver to tighten each pump head bolt to 98 inch-pounds of torque. 15. Power-off the p-ism. 16. Reattach all fittings and seal-wash tubing. Tip: When reattaching the outlet tubing to the transducer, tighten the inlet-tubing fitting finger-tight plus as much as 1/6-turn, for existing fittings, or 3/4-turn for new fittings. 17. Orient the i 2 Valve assembly so that the cable exits from the left-hand side. 18. Insert the i 2 Valve assembly into the bottom of the primary pump head, and route the cable behind the valve actuator. 19. Finger-tighten the shell nut, rotating it approximately 5 full turns, to secure the valve. 20. Use the 5/16-inch open-end wrench to tighten the nut an additional 1/8-turn. Place 5/16-inch open-end wrench here 94 October 13, 2014, Rev. B

95 Removing the primary head and replacing its seals 21. Reinsert the low pressure filter and tube into the knurled adapter fitting and finger-tighten. Low pressure filter Adapter fitting 22. Align the white arrow on the i 2 Valve connector with the white arrow on the receptacle, in the 12 o clock position, and insert the connector into the receptacle. White arrows Connector receptacle i 2 Valve connector 23. Return the solvent bottles to their original location. 24. Power-on the p-ism. 25. Prime the p-ism (see page 37). 26. Perform the ISM leak test (see the Instrument console online Help). If the leak test results are not satisfactory, pressurize the head plunger seals to properly seat them. October 13, 2014, Rev. B 95

96 3 Maintenance Procedures To pressurize the seals: Run the p-ism at 96,527 kpa (965 bar, 14,000 psi) for a half-hour, or run the leak test until results are satisfactory. Removing the accumulator head and replacing its seals See the Instrument console online Help to help determine whether you need to replace the accumulator plunger seals. Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when removing and replacing the plunger seals. Required materials Compressed air Gloves: clean, powder-free, chemical-resistant Methanol Plunger (recommended) Plunger seal and plunger seal spacer Fluoropolymer O-ring Seal-wash seal Required tools 1/4-inch open-end wrench 5/16-inch open-end wrench Plunger removal tool (recommended) Sharp tool T27 TORX driver (startup kit) 96 October 13, 2014, Rev. B

97 Removing the accumulator head and replacing its seals Torque wrench To remove the accumulator head: 1. Flush the p-ism with nonhazardous solvent. 2. In the Instrument console, select Isocratic Solvent Manager from the system tree. 3. In the Isocratic Solvent Manager information window, click Maintain > Heads. 4. In the Head Maintenance dialog box, select the accumulator head. 5. Click Move Backward, and then wait for the plunger to stop. Caution: To avoid damaging electrical parts, never disconnect an electrical assembly while power is applied to an instrument or device. To completely interrupt power, set the power switch to Off, and then unplug the power cord from the AC source. Wait 10 seconds thereafter before you disconnect an assembly. 6. Power-off the p-ism. Warning: To avoid injuries arising from contact with spilled solvent (siphoning), move the solvent bottles to a location below the p-ism. 7. Move the solvent bottles to a location below the p-ism. October 13, 2014, Rev. B 97

98 3 Maintenance Procedures 8. Remove the seal-wash tubing secured to the seal-wash housing. Seal-wash tubing Seal-wash tubing 9. Using the 1/4-inch open-end wrench, disconnect the outlet tubing from the transducer. Transducer Outlet tubing 98 October 13, 2014, Rev. B

99 Removing the accumulator head and replacing its seals 10. Using the 5/16-inch open-end wrench to hold the check-valve cartridge in place, disconnect the tubing connection from the check valve with the 1/4-inch open-end wrench. Accumulator check valve Place 5/16-inch open-end wrench here Compression fitting 11. Disconnect the pressure transducer cable from the bulkhead by squeezing on the tabs and pulling gently. Pressure transducer cable connector Tabs October 13, 2014, Rev. B 99

100 3 Maintenance Procedures 12. Using the T27 TORX driver, loosen the 2 head bolts 1/2-turn. Tip: The bolts are accessible from the front of the pressure transducer. Head bolt (2) 100 October 13, 2014, Rev. B

101 Removing the accumulator head and replacing its seals Caution: To avoid damaging the plunger, support the pump head from below as you remove it. 13. Using the T27 TORX driver, loosen and remove the 2 support plate bolts, and then gently pull the head and support plate off the actuator housing, making sure not to tilt the head during the extraction. Support plate bolt (2) Plunger Head and support plate October 13, 2014, Rev. B 101

102 3 Maintenance Procedures Warning: To avoid hand lacerations, use care when removing the old plunger. Bending the plunger shaft can cause it to break. 14. Use the recessed side of the plunger removal tool to apply pressure to both sides of the release collar, and then remove the old plunger. Recessed lip of plunger removal tool TP02990 Spring-loaded release collar Plunger Plunger removal tool TP Remove the plunger removal tool from the release collar. 102 October 13, 2014, Rev. B

103 Removing the accumulator head and replacing its seals To remove the accumulator plunger seals: 1. Stand the head upright on a clean surface. 2. Using the T27 TORX driver, completely loosen the 2 head bolts to release the support plate from the pump head. Requirement: If you remove the transducer and head bolts, be sure to reuse the head bolt washers when reassembling the pump head. Head bolt access hole (2) 3. Lift the pump head from the support plate. Pump head Seal-wash housing Support plate October 13, 2014, Rev. B 103

104 3 Maintenance Procedures 4. Lift the seal-wash housing from the support plate. Seal-wash housing Support plate 5. Pull the seal wash seal from the seal-wash housing. Seal-wash seal 6. Carefully withdraw the plunger seal and spacer. Plunger seal 104 October 13, 2014, Rev. B

105 Removing the accumulator head and replacing its seals 7. Taking care not to scratch any surface, use a sharp tool to remove the fluoropolymer O-ring. 8. Inspect the seal-wash housing surface and pump head surface ensuring both are free from scratches and particulates. 9. Lubricate the new fluoropolymer O-ring with methanol, and press the O-ring into its seat with your thumbs. 10. Lubricate the new plunger seal spacer with methanol and place it in the seal-wash housing. Plunger seal Caution: To avoid scratching any metal surfaces, use care when using a sharp tool to remove the fluoropolymer O-ring from the pump head. Seal-wash housing 11. Spray the new plunger seal with compressed air to remove any particulates. October 13, 2014, Rev. B 105

106 3 Maintenance Procedures 12. Orient the seal-wash housing so that the holes on its side align with the holes on the side of the pump head, and then guide it into place. Seal-wash housing Pump head 13. Spray the seal-wash seal with compressed air to remove any particulates. 14. Lubricate the new seal-wash seal with methanol, place it in the seal-wash housing, and press it into place. Seal-wash seal 106 October 13, 2014, Rev. B

107 Removing the accumulator head and replacing its seals 15. Place the support plate on top of the pump head, ensuring the round side of the plate is oriented toward the bottom side of the head. Round side of support plate Bottom side of pump head 16. Holding the assembly together, using the T27 TORX driver, fully tighten the 2 head bolts until the screws bottom out and then unscrew 1/4 turn. T27 TORX driver Head bolt access hole Head bolt access hole TP03388 October 13, 2014, Rev. B 107

108 3 Maintenance Procedures To reattach the accumulator head: 1. Flip the assembly over, and then lubricate the seals with methanol. Seals 2. Carefully insert the sapphire plunger shaft into the pump head until the plunger shaft is no longer visible, ensuring the shaft does not contact the support plate. Recommendation: Replace the plunger whenever you replace the plunger seal. Plunger shaft Support plate 108 October 13, 2014, Rev. B

109 Removing the accumulator head and replacing its seals Caution: To avoid damaging the plunger, ensure that the head assembly is not tilted when you position it onto the actuator housing. 3. Carefully slide the head assembly and sapphire plunger into the actuator housing, making sure not to tilt the head. 4. Hold the head assembly securely against the actuator housing, and then use the T27 TORX driver to tighten the support plate bolts securely. Support plate bolt (2) 5. Connect the pressure transducer cable to the bulkhead. 6. Power-on the p-ism. 7. In the Instrument console, select Isocratic Solvent Manager from the system tree. 8. In the ISM information window, click Maintain > Heads. 9. In the Head Maintenance dialog box, select the accumulator head. 10. Click Move Forward, and then wait for the piston to engage the plunger sphere. Caution: To avoid damaging the plunger, alternately tighten the the support plate screws 1/4-turn so that they are uniformly torqued. 11. Use the torque driver to tighten each pump head bolt to 40 inch-pounds of torque. October 13, 2014, Rev. B 109

110 3 Maintenance Procedures 12. After tightening each pump head bolt to 40 inch-pounds of torque, use the torque driver to tighten each pump head bolt to 60 inch-pounds of torque. 13. After tightening each pump head bolt to 60 inch-pounds of torque, use the torque driver to tighten each pump head bolt to 80 inch-pounds of torque. 14. After tightening each pump head bolt to 80 inch-pounds of torque, use the torque driver to tighten each pump head bolt to 98 inch-pounds of torque. 15. Reattach all fittings and seal-wash tubing. Tip: When reattaching the outlet tubing to the transducer, tighten the inlet-tubing fitting finger-tight plus as much as 1/6-turn, for existing fittings, or 3/4-turn for new fittings. 16. Return the solvent bottles to their original location. 17. Prime the p-ism (see page 37). 18. Perform the p-ism leak test (see the ACQUITY APC online Help). If the leak test results are not satisfactory, pressurize the head plunger seals to properly seat them. To pressurize the seals: Run the p-ism at 96,527 kpa (965 bar, 14,000 psi) for a half-hour, or run the leak test until results are satisfactory. Cleaning the instrument s exterior Clean surfaces of the solvent manager using only a clean, soft, lint-free paper or clean cloth dampened with water. 110 October 13, 2014, Rev. B

111 Cleaning the instrument s exterior Warning: To avoid electric shock, observe these precautions: Disconnect the device or instrument from the electrical supply. When cleaning the surface of a device or instrument, apply water to a cloth, and then wipe the unit. Do not spray or otherwise directly apply water to any device surface. Warning: To avoid personal injury, use eye and hand protection during the cleaning process. October 13, 2014, Rev. B 111

112 3 Maintenance Procedures 112 October 13, 2014, Rev. B

113 A Safety Advisories Waters instruments and devices display hazard symbols that alert you to the hidden dangers associated with a product s operation and maintenance. The symbols also appear in product manuals where they accompany statements describing the hazards and advising how to avoid them. This appendix presents the safety symbols and statements that apply to all of the products that Waters offers. Contents: Topic Page Warning symbols Caution advisory Warnings that apply to all Waters instruments and devices Electrical and handling symbols October 13, 2014, Rev. B 113

114 A Safety Advisories Warning symbols Warning symbols alert you to the risk of death, injury or seriously adverse physiological reactions associated with an instrument s use or misuse. Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices. The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device, or a component of an instrument or device. When one of these symbols appear in a manual s narrative sections or procedures, an accompanying statement identifies the applicable risk and explains how to avoid it. Warning: (General risk of danger. When this symbol appears on an instrument, consult the instrument s user documentation for important safety-related information before you use the instrument.) Warning: (Risk of burn injury from contacting hot surfaces.) Warning: (Risk of electric shock.) Warning: (Risk of fire.) Warning: (Risk of sharp-point puncture injury.) Warning: (Risk of hand crush injury.) Warning: (Risk of injury caused by moving machinery.) Warning: (Risk of exposure to ultraviolet radiation.) Warning: (Risk of contacting corrosive substances.) Warning: (Risk of exposure to a toxic substance.) Warning: (Risk of personal exposure to laser radiation.) 114 October 13, 2014, Rev. B

115 Warning symbols Warning: (Risk of exposure to biological agents that can pose a serious health threat.) Warning: (Risk of tipping.) Warning: (Risk of explosion.) Warning: (Risk of eye injury.) Specific warnings The following warnings (both symbols and text) can appear in the user manuals of particular instruments and devices and on labels affixed to them or their component parts. Good laboratory practices warning Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the p-ism. Consult the Material Safety Data Sheets regarding the solvents you use. Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans. Warning: To avoid infection with potentially infectious, human-sourced products, inactivated microorganisms, and other biological materials, assume that all biological fluids that you handle are infectious. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, Biosafety in Microbiological and Biomedical Laboratories (BMBL). Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the biohazard safety representative for your organization regarding the proper use and handling of infectious substances. October 13, 2014, Rev. B 115

116 A Safety Advisories Biohazard and chemical hazard warning This warning applies to Waters instruments and devices that can process biohazards, corrosive materials, or toxic materials. Warning: To avoid personal contamination with biohazards, toxic materials, or corrosive materials, you must understand the hazards associated with their handling. Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Disposal of Chemicals. Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the safety representative for your organization regarding its protocols for handling such materials. Caution advisory Caution advisories appear where an instrument or device can be subject to use or misuse that can damage it or compromise a sample s integrity. The exclamation point symbol and its associated statement alert you to such risk. Caution: To avoid damaging the instrument s case, do not clean it with abrasives or solvents. 116 October 13, 2014, Rev. B

117 Warnings that apply to all Waters instruments and devices Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment. Important: Toute modification sur cette unité n ayant pas été expressément approuvée par l autorité responsable de la conformité à la réglementation peut annuler le droit de l utilisateur à exploiter l équipement. Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen. Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all'utilizzo dell'apparecchiatura da parte dell'utente. Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo. 注意 : 未經有關法規認證部門允許對本設備進行的改變或修改, 可能會使使用者喪失操作該設備的權利 注意 : 未经有关法规认证部门明确允许对本设备进行的改变或改装, 可能会使使用者丧失操作该设备的合法性 주의 : 규정준수를책임지는당사자의명백한승인없이이장치를개조또는변경할경우, 이장치를운용할수있는사용자권한의효력을상실할수있습니다. 注意 : 規制機関から明確な承認を受けずに本装置の変更や改造を行うと 本装置のユーザーとしての承認が無効になる可能性があります October 13, 2014, Rev. B 117

118 A Safety Advisories Warning: Use caution when working with any polymer tubing under pressure: Always wear eye protection when near pressurized polymer tubing. Extinguish all nearby flames. Do not use tubing that has been severely stressed or kinked. Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids. Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic tubing to swell, which greatly reduces the rupture pressure of the tubing. Attention: Manipulez les tubes en polymère sous pression avec precaution: Portez systématiquement des lunettes de protection lorsque vous vous trouvez à proximité de tubes en polymère pressurisés. Eteignez toute flamme se trouvant à proximité de l instrument. Evitez d'utiliser des tubes sévèrement déformés ou endommagés. Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane (THF) ou de l'acide sulfurique ou nitrique concentré. Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture. Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht: In der Nähe von unter Druck stehenden Polymerschläuchen stets Schutzbrille tragen. Alle offenen Flammen in der Nähe löschen. Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind. Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder konzentrierte Salpeter- oder Schwefelsäure verwenden. Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert. 118 October 13, 2014, Rev. B

119 Warnings that apply to all Waters instruments and devices Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. Spegnere tutte le fiamme vive nell'ambiente circostante. Non utilizzare tubi eccessivamente logorati o piegati. Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico o nitrico concentrati. Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di rottura dei tubi stessi. Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión: El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos de polímero sometidos a presión. Si hubiera alguna llama las proximidades. No se debe trabajar con tubos que se hayan doblado o sometido a altas presiones. Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano (THF) o ácidos nítrico o sulfúrico concentrados. Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos. 警告 : 當在有壓力的情況下使用聚合物管線時, 小心注意以下幾點 當接近有壓力的聚合物管線時一定要戴防護眼鏡 熄滅附近所有的火焰 不要使用已經被壓癟或嚴重彎曲管線 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸 要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹, 大大降低管線的耐壓能力 October 13, 2014, Rev. B 119

120 A Safety Advisories 警告 : 当有压力的情况下使用管线时, 小心注意以下几点 : 当接近有压力的聚合物管线时一定要戴防护眼镜 熄灭附近所有的火焰 不要使用已经被压瘪或严重弯曲的管线 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀, 大大降低管线的耐压能力 경고 : 가압폴리머튜브로작업할경우에는주의하십시오. 가압폴리머튜브근처에서는항상보호안경을착용하십시오. 근처의화기를모두끄십시오. 심하게변형되거나꼬인튜브는사용하지마십시오. 비금속 (Nonmetallic) 튜브를테트라히드로푸란 (Tetrahydrofuran: THF) 또는농축질산또는황산과함께사용하지마십시오. 염화메틸렌 (Methylene chloride) 및디메틸술폭시드 (Dimethyl sulfoxide) 는비금속튜브를부풀려튜브의파열압력을크게감소시킬수있으므로유의하십시오. 警告 : 圧力のかかったポリマーチューブを扱うときは 注意してください 加圧されたポリマーチューブの付近では 必ず保護メガネを着用してください 近くにある火を消してください 著しく変形した または折れ曲がったチューブは使用しないでください 非金属チューブには テトラヒドロフラン (THF) や高濃度の硝酸または硫酸などを流さないでください 塩化メチレンやジメチルスルホキシドは 非金属チューブの膨張を引き起こす場合があり その場合 チューブは極めて低い圧力で破裂します 120 October 13, 2014, Rev. B

121 Warnings that apply to all Waters instruments and devices Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: L utilisateur doit être informé que si le matériel est utilisé d une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d être défectueuses. Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwenddung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren. Attenzione: si rende noto all'utente che l'eventuale utilizzo dell'apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall'apparecchiatura. Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes. 警告 : 使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用, 那麼該設備所提供的保護將被消弱 警告 : 使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用, 那么该设备所提供的保护将被削弱 경고 : 제조업체가명시하지않은방식으로장비를사용할경우장비가제공하는보호수단이제대로작동하지않을수있다는점을사용자에게반드시인식시켜야합니다. 警告 : ユーザーは 製造元により指定されていない方法で機器を使用すると 機器が提供している保証が無効になる可能性があることに注意して下さい October 13, 2014, Rev. B 121

122 A Safety Advisories Electrical and handling symbols Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse 122 October 13, 2014, Rev. B