Auxiliary Solvent Manager Overview and Maintenance Guide

Transcription

1 Auxiliary Solvent Manager Overview and Maintenance Guide / Revision A Copyright Waters Corporation 2014 All rights reserved

2 ii February 6, 2014, Rev. A

3 General Information Copyright notice 2014 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER. The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com). Trademarks Connections INSIGHT, MassLynx, UPLC, THE SCIENCE OF WHAT S POSSIBLE., and Waters are registered trademarks of Waters Corporation. Milli-Q is a registered trademark of Merck KGaA Darmstadt Germany. Optima is a trademark of Fisher Scientific, Inc. PEEK is a trademark of Victrex Corporation. PharMed is a registered trademark of Saint-Gobain Ceramics & Plastics, Inc. TORX is a registered trademark of Textron Corporation. Other registered trademarks or trademarks are the sole property of their owners. Customer comments Waters Technical Communications organization invites you to report any errors that you encounter in this document or to suggest ideas for otherwise improving it. Help us better understand what you expect from our February 6, 2014, Rev. A iii

4 documentation so that we can continuously improve its accuracy and usability. We seriously consider every customer comment we receive. You can reach us at Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information: Contacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide. Visit Telephone and fax From the USA or Canada, phone , or fax For other locations worldwide, phone and fax numbers appear in the Waters Web site. Conventional mail Waters Corporation 34 Maple Street Milford, MA USA Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical, biological, or radiological hazards (or any combination thereof). You must know the potentially hazardous effects of all substances you work with. Always follow Good Laboratory Practice, and consult your organization s safety representative for guidance. iv February 6, 2014, Rev. A

5 Considerations specific to the Auxiliary Solvent Manager High voltage hazard Warning: To avoid electric shock, do not remove the device s protective panels. The components within are not user-serviceable. FCC radiation emissions notice Changes or modifications not expressly approved by the party responsible for compliance, could void the users authority to operate the equipment. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. Canada spectrum management emissions notice This class A digital product apparatus complies with Canadian ICES-001. Cet appareil numérique de la classe A est conforme à la norme NMB-001. Electrical power safety notice Do not position the instrument so that it is difficult to operate the disconnecting device. Safety hazard symbol notice Documentation needs to be consulted in all cases where the symbol is used to find out the nature of the potential hazard and any actions which have to be taken. Equipment misuse notice If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. February 6, 2014, Rev. A v

6 Safety advisories Consult Appendix A for a comprehensive list of warning and caution advisories. Operating this device When operating this device, follow standard quality-control (QC) procedures and the guidelines presented in this section. Applicable symbols Symbol Definition Manufacturer Authorized representative of the European Community Confirms that a manufactured product complies with all applicable European Community directives Australia C-Tick EMC compliant Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements Consult instructions for use Electrical and electronic equipment with this symbol may contain hazardous substances and should not be disposed of as general waste. For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions. vi February 6, 2014, Rev. A

7 Audience and purpose This guide is intended for use by individuals who operate and maintain the Auxiliary Solvent Manager (ASM). Intended use of the Auxiliary Solvent Manager Waters designed the ASM for use in ultra-performance liquid chromatography applications. The ASM is not intended for use in diagnostic applications. Calibrating To calibrate LC systems, follow acceptable calibration methods using at least five standards to generate a standard curve. The concentration range for standards should include the entire range of QC samples, typical specimens, and atypical specimens. Quality-control Routinely run three QC samples that represent subnormal, normal, and above-normal levels of a compound. Ensure that QC sample results fall within an acceptable range, and evaluate precision from day to day and run to run. Data collected when QC samples are out of range might not be valid. Do not report these data until you are certain that the instrument performs satisfactorily. ISM classification ISM Classification: ISM Group 1 Class B This classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and Medical (ISM) instruments requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radio-frequency energy that is necessary for the internal functioning of the equipment. Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network. February 6, 2014, Rev. A vii

8 EC authorized representative Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX UK Telephone: Fax: Contact: Quality manager viii February 6, 2014, Rev. A

9 Table of Contents General Information... iii Copyright notice... iii Trademarks... iii Customer comments... iii Contacting Waters... iv Safety considerations... iv Considerations specific to the Auxiliary Solvent Manager... v FCC radiation emissions notice... v Canada spectrum management emissions notice... v Electrical power safety notice... v Safety hazard symbol notice... v Equipment misuse notice... v Safety advisories... vi Operating this device... vi Applicable symbols... vi Audience and purpose... vii Intended use of the Auxiliary Solvent Manager... vii Calibrating... vii Quality-control... vii ISM classification... vii ISM Classification: ISM Group 1 Class B... vii EC authorized representative... viii 1 Auxiliary Solvent Manager Workings Integrated flow control module Intake valves February 6, 2014, Rev. A ix

10 Maintenance and diagnostic functions Major components Flow path through the solvent management system Preparing for Operation Stacking system modules Installing the waste and degasser vent tubing Connecting to the solvent supply Installation recommendations for fittings Making Ethernet connections Making signal connections Connecting to the electricity source Preparing the ASM Priming the seal wash Priming the ASM Priming a dry ASM Using the Auxiliary Solvent Manager Auto zeroing the flow transducers Inspecting for leaks Maintenance Procedures Maintaining the ASM Contacting Waters technical service Maintenance schedule Spare parts Maintenance considerations Configuring maintenance warnings Troubleshooting with Connections INSIGHT x February 6, 2014, Rev. A

11 Replacing solvent reservoir filters Cleaning the air filter in the door Replacing the air filter in the door Replacing the solvent filters Replacing the vent valve cartridge Replacing the i 2 Valve actuator Replacing the i 2 Valve cartridge Replacing the in-line filter cartridge on the i 2 Valve actuator Replacing the accumulator check valve Replacing the primary head plunger and seals Replacing the accumulator head plunger and seals Cleaning the exterior of the ASM A Safety Advisories Warning symbols Specific warnings Caution advisory Warnings that apply to all Waters instruments and devices Electrical and handling symbols Electrical symbols Handling symbols B Specifications Physical specifications Environmental specifications Electrical specifications Input/output specifications February 6, 2014, Rev. A xi

12 Performance specifications Wetted materials of construction xii February 6, 2014, Rev. A

13 Workings 1 Auxiliary Solvent Manager The Auxiliary Solvent Manager (ASM) is a high-pressure pump that provides accurate pulse-free flow rates at operating pressures as high as 103,421 kpa (1034 bar, 15,000 psi). Workings Contents: Topic Page Workings Integrated flow control module Intake valves Maintenance and diagnostic functions Major components Flow path through the solvent management system Each of the ASM s two independent pump systems, A (on the left-hand side) and B (on the right-hand side), contains two linear-drive actuators. Each left-hand and right-hand actuator pair comprises a single reciprocating serial pump that delivers precise flow of a single solvent. The solvent flows to the µsm-fl or column at an overall flow rate of 0.0 to µl/min from pump A and 0.0 to µl/min from pump B. Pump B utilizes a flow sensor that allows for closed loop flow control at low flow rates. The data acquired from both the pressure transducers of both pumps and the flow sensor of pump B are used by the firmware to produce accurate and pressure pulse free solvent flow. In doing so, it ensures a consistent manner of solvent delivery and minimizes pump-induced disturbances that can adversely affect chromatographic performance. February 6, 2014, Rev. A 13

14 1 Auxiliary Solvent Manager Integrated flow control module The ASM s integrated flow control module regulates the solvent flow in the pump B solvent channel and provides accurate flow for these solvents and pre-mixed binary-solvent combinations: Water Acetonitrile Methanol Isopropanol Water/acetonitrile Water/methanol Water/isopropanol Acetonitrile/methanol Acetonitrile/isopropanol Methanol/isopropanol Flow rates are calibrated using one solvent and compensated to provide accurate flow for other solvents. The module s pump B flow sensor measures the flow. The ASM monitors the output and adjusts the solvent flow. Intake valves The primary intake head of each pump in the ASM is fitted with a Waters Intelligent Intake Valve (i 2 Valve). Electronically controlled and electrically actuated, the valves open and close with each pump cycle. Maintenance and diagnostic functions The software supports the operation of the ASM and all other system modules via the Console, a graphical, real-time, software interface that monitors system status. Using the instrument console software, you directly control the system, troubleshoot errors, and perform automated maintenance functions. For example, to measure pump performance, you monitor changes in system backpressure by evaluating the performance of each pump head. The dynamic behavior of the pump pressure traces can help isolate the likely cause, a 14 February 6, 2014, Rev. A

15 Major components malfunctioning check valve or failed seal. You could then select the appropriate maintenance option for replacing the failed component. The instrument console software also serves as the interface for Waters Connections INSIGHT, which offers remote service and alert capabilities. Through Connections INSIGHT, Waters technicians can swiftly respond to any alarms or error alerts initiated by system components. Finally, the instrument console software provides the interface for particular functions, like generating a service profile, that you can send directly to the Waters Global Service and Support organization for analysis and interpretation. Major components The diagram and the table directly following it show and explain the function of ASM s major components. Primary pump A Check valve (2) Accumulator pump A Primary pump B Accumulator pump B Check valve in-line filter (2) i 2 Valve actuator A Solvent selection valve (2) Pump drip tray waste line i 2 Valve actuator B Flow control module (ASM shown) 6-channel degasser chamber Seal-wash waste tubing Degasser vent tubing Drip tray Seal-wash pump Vent valve Solvent filters (2) Solvent vent tubing Drain fitting (to waste) February 6, 2014, Rev. A 15

16 1 Auxiliary Solvent Manager ASM components: Component Accumulator pump (A and B) Check valve Check valve in-line filter 6-channel degasser chamber Degasser vent tubing Drain fitting (to waste) Drip tray Flow control module i 2 Valve Solvent filters (2) Primary pump (A and B) Pump drip tray waste line Seal-wash pump Seal-wash waste tubing Description Receives solvent from the primary pump, and delivers it to the system. A dual-ball check valve that allows flow in only one direction. Removes particulates from the incoming solvent. Removes dissolved gasses from mobile-phase solvents and exhausts them, and any condensates, through waste tubing. Notes: Vacuum degassing can change eluent composition at microscale flow rates. The A1 and B1 solvent lines do not pass through the degasser. Vents exhaust from the degasser pump. Routes waste from the leak and waste management system flow through this connector to the waste container. Manages fluid leaks. Monitors the solvent flow in the pump B solvent channel. An electronically controlled check valve that allows flow in only one direction. Filters the solvent before it reaches the µsm-fl. Draws solvent, transferring it to the accumulator pump and system as part of the serial flow design. Transfers leaks from the internal drip tray to the external drip tray. Circulates solvent, to keep the actuator s high-pressure seals and plungers free of contaminants. Directs seal-wash waste to the drip tray. 16 February 6, 2014, Rev. A

17 Flow path through the solvent management system ASM components: (Continued) Component Solvent selection valve Solvent vent tubing Vent valve Description Enables the selection of one solvent from A1 or A2 and one solvent from B1 or B2. Tubing that vents solvent to waste during priming. A 3-position valve that automatically switches to the waste/vent position during priming or column switching, the block position during the ASM maintenance leak test, and the system position during normal operation (or during the system leak test). Flow path through the solvent management system This diagram shows the ASM s flow: Primary pump A Accumulator pump A Primary pump B Accumulator pump B Solvent selection valves Vent valve A Filter Seal-wash pump Degasser Filter Flow sensor B Sealwash Waste February 6, 2014, Rev. A 17

18 1 Auxiliary Solvent Manager Solvent flow sequence: 1. The solvent is stored in solvent reservoirs A1, A2, B1, and B2. 2. The in-line vacuum degasser degasses the solvent in solvent reservoirs A2 and B2 (strong needle wash and weak needle wash). 3. The solvent selection valve selects solvents for pump A and pump B from solvent reservoirs A1, A2, B1, and B2. 4. The solvents flow through the i 2 Valve, an electronically operated check valve located on the inlet of the primary pump s actuator. 5. The primary piston fills the accumulator pump. 6. The accumulator pump s piston delivers solvent to the vent valve. 7. From the vent valve, the solvent flows to the in-line filters and then to the flow control module. 18 February 6, 2014, Rev. A

19 2 Preparing for Operation For optimal performance of the system, you prepare the ASM by first priming the seal-wash system and then the ASM itself. Contents: Topic Page Stacking system modules Installing the waste and degasser vent tubing Connecting to the solvent supply Installation recommendations for fittings Making Ethernet connections Making signal connections Connecting to the electricity source Preparing the ASM Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. To maintain the efficiency of the ASM and to obtain accurate, reproducible chromatograms, use only MS-grade solvents and additives. For details, see the solvent considerations document for your system. Caution: To avoid damaging components of the ASM, do not use toluene or halogenated solvents such as chloroform or methylene chloride. February 6, 2014, Rev. A 19

20 2 Preparing for Operation Stacking system modules Warning: To avoid spinal and muscular injury, do not attempt to lift the ASM without assistance. To stack the modules: 1. Place the ASM atop the system bench or optional microscale cart. 2. Place the rear feet of the next module in the system stack atop the ASM, and slide the added module backward until its rear alignment pin rests in the rear alignment slot on the ASM. Alignment pin (2) Alignment slot (2) 3. Lower the front of the added module so that its front alignment pin rests in the front alignment slot on the ASM. 4. Repeat step 2 and step 3 for the remaining system modules. 20 February 6, 2014, Rev. A

21 Installing the waste and degasser vent tubing Installing the waste and degasser vent tubing Warning: To avoid personal contact with biohazards or toxic materials, wear clean, chemical-resistant, powder-free gloves when installing or removing the waste and degasser vent tubing. Required materials Degasser vent tubing Gloves: clean, powder-free, chemical-resistant Waste tubing To install the waste and degasser vent tubing: Caution: To avoid distorting the drip tray or causing the drain cup to leak, restrain the drain cup when attaching or removing the waste tubing. 1. Wet the barbed drain fitting, located at the bottom of the ASM using methanol. 2. Holding the back of the drain cup, slide the waste tubing over the barbed drain fitting, and route the tubing to a suitable waste container. Drip tray Barbed drain fitting Drain cup Waste tubing Degasser vent tubing 3. Route the degasser vent tubing to a suitable waste container. February 6, 2014, Rev. A 21

22 2 Preparing for Operation Warning: To avoid releasing solvent vapors into the room, route vent tubing from the waste container to a fume hood or other, suitable exhaust system. Warning: To avoid spills, empty the waste container at regular intervals. Caution: To avoid fluid backup, you must ensure proper drainage of waste: Place the waste container below the system stack. Ensure the waste tubing does not crimp or bend. A crimp or bend can impede flow to the waste container. Ensure the exit of the waste tubing is not immersed in waste solvent. If necessary, shorten the tubing so that no portion of it drops below the top of the waste container. Caution: To avoid pressure buildup in the vent tubing of the waste container, ensure the tubing does not crimp or bend sharply. The tubing must freely vent gas and vapor to a fume hood or laboratory exhaust system. 22 February 6, 2014, Rev. A

23 Connecting to the solvent supply Correct positioning of waste and degasser vent tubing: Waste tubing (from stack of system modules) Location of degasser vent tubing (from stack of system modules) Waste container vent tubing (to fume hood) Waste container TP03477 Connecting to the solvent supply Connect the solvent-supply plumbing before you connect the capillary (fused silica) tubing. Doing so lets you flush the capillary tubing as you connect it. Flush the system with the appropriate solvents before passing eluent into the column, optical detector, or mass spectrometer. Important: Do not operate the system using mobile phases of ph greater than 10. Alkaline solutions, such as ammonium hydroxide (ph 11), can react with glass, etching solvent bottles and silica capillary tubing. The etching causes an elevated chemical background that a mass spectrometer can detect. February 6, 2014, Rev. A 23

24 2 Preparing for Operation Passivation For optimal mass-spectrometry results, passivate solvent bottles or reservoirs, cleaning them thoroughly before adding solvents to them. Do not use detergent, and do not wash the bottles in a mechanical dishwasher together with other glassware. Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. To passivate the solvent bottles: 1. Sonicate the bottles in nitric acid, 20% to 30%, for approximately 30 minutes. 2. Rinse the bottles thoroughly with Milli-Q water. To connect the solvent supply: 1. Choose solvent reservoirs that snugly fit the reservoir caps supplied in the startup kit. Requirement: Do not pressurize solvent reservoirs. To ensure proper gravity flow, maintain an adequate head pressure by placing the reservoirs in the solvent tray at the top of the system stack. Recommendation: Use 500-mL solvent reservoirs, Caution: To avoid contaminating the solvent filter with skin oils, wear clean, chemical-resistant, powder-free gloves when handling it. 2. Fill the solvent reservoirs with the appropriate MS-grade solvents. 3. Place the solvent supply bottles in the solvent tray atop the system stack. Rationale: This placement maintains a head pressure of solvent, ensuring proper gravity flow. 24 February 6, 2014, Rev. A

25 Connecting to the solvent supply 4. Unroll the factory-plumbed solvent inlet lines, and feed them through the vertical tubing guide at the right-hand, front, corner of the modules. ASM s vertical tubing guide: Tubing guide 5. Insert the solvent tubing into the solvent bottles, in the tray atop the system instrument stack. Solvent tubing Solvent bottles Solvent tray TP Repeat step 5 for the remaining solvent supply lines. February 6, 2014, Rev. A 25

26 2 Preparing for Operation 7. Plug any unused solvent inlet lines at the in-line degasser or unused degasser solvent outlet lines with pin plugs (included in the startup kit). 8. Plumb the system drain s waste line to a waste vessel (see page 21). Installation recommendations for fittings Warning: To avoid personal contact with fittings contaminated with biohazards or toxic materials, wear clean, chemical-resistant, powder-free gloves when reinstalling the fittings. The system uses gold-plated compression fittings and two-piece ferrules. Note the diagram below for assembly orientation. Example ferrule and compression fitting: Ferrule with locking ring Compression fitting Tubing Warning: To avoid eye injury when flushing the tubing, always wear safety glasses, and direct the tubing end away from you. Recommendations: Install capillary tubing as follows: 1. Connect the tubing end nearest the pump source (the active end). 2. In the Console, select Auxiliary Solvent Manager from the system tree. 3. Click Troubleshoot > Set Pressure Diagnostic, and then choose the Advanced option. 4. Configure both pumps, A and B, for constant pressure, so that each pump contributes equally to the flow volume. 5. Set the pressure to 17,237 kpa (172 bar, 2500 psi) until solvent flows from the end of the tubing. 26 February 6, 2014, Rev. A

27 Installation recommendations for fittings 6. Finally, flush the tubing for a minimum of 2 additional minutes, to remove any particles from the system. For more information about the set-pressure diagnostic test, see the online Help. After connecting both ends of the tubing, whenever the downstream portion of the new tubing is subject to blocking by means of a valve or other device, perform the set-pressure diagnostic test (see the online Help for details), to determine whether leaks are present. If you remove a fitting connection: 1. Connect the tubing end nearest the pump source (the active end). 2. In the Console, select Auxiliary Solvent Manager from the system tree. 3. Click Troubleshoot > Set Pressure Diagnostic, and then choose the Advanced option. 4. Configure both pumps, A and B, for constant pressure, so that each pump contributes equally to the flow volume. 5. Set the pressure to 17,237 kpa (172 bar, 2500 psi) until solvent flows from the end of the tubing. 6. Flush the tubing for a minimum of 2 additional minutes, to remove any particles from the system. 7. Finally, perform the set-pressure diagnostic test, to determine whether leaks are present. For more information about the set pressure diagnostic test, see the online Help. If you replace tubing, note that installing new tubing can cause the release of particles into the system. To avoid purging particles into a sensitive component, such as a column: 1. Connect the tubing end nearest the pump source (the active end). 2. In the Console, select Auxiliary Solvent Manager from the system tree. 3. Click Troubleshoot > Set Pressure Diagnostic, and then choose the Advanced option. 4. Configure both pumps, A and B, for constant pressure, so that each pump contributes equally to the flow volume. February 6, 2014, Rev. A 27

28 2 Preparing for Operation 5. Set the pressure to 17,237 kpa (172 bar, 2500 psi) until solvent flows from the end of the tubing. 6. Finally, flush the tubing for a minimum of 2 additional minutes, to remove any particles from the system. For more information about the set-pressure diagnostic test, see the online Help. To prevent bandspreading, ensure tubing fully bottoms in its fitting hole before tightening compression fittings. For easier accessibility, use compression fittings with long flats to attach tubes to the vent valve. Whenever you loosen fittings during maintenance, examine them for cracks, stripped threads, and deformations. Do not reseat stainless steel ferrules more than six times. Required material Gloves: clean, powder-free, chemical-resistant 28 February 6, 2014, Rev. A

29 Installation recommendations for fittings When tightening system fittings, consult the following table. Installation recommendations for ASM fittings: Fitting Long 1/4-28 fitting with flangeless ferrule and stainless steel lock ring, installed on 1/8-inch outside diameter (OD) tubing First use or reinstalled Short 1/4-28 fitting with flangeless ferrule and stainless steel lock ring, installed on inch OD tubing First use or reinstalled End of lock ring with smaller inside diameter (ID) Lock ring Ferrule End of lock ring with smaller ID Lock ring Ferrule TP03377 TP03379 Recommended tightening Finger-tight Finger-tight February 6, 2014, Rev. A 29

30 2 Preparing for Operation Installation recommendations for ASM fittings: (Continued) Fitting V-detail stainless steel (gold-plated) fitting with long flats and a 2-piece stainless steel ferrule (designed for use with stainless steel tubing) Stainless steel tubing Recommended tightening Finger-tight, plus 3/4-turn using wrench. 3/4-turn First use Long flats Stainless steel tubing TP piece ferrule Finger-tight, plus as much as 1/6-turn using wrench. 1/6-turn Reinstalled Long flats TP piece ferrule 30 February 6, 2014, Rev. A

31 Installation recommendations for fittings Installation recommendations for ASM fittings: (Continued) Fitting V-detail stainless steel (gold-plated) fitting with short flats and a 2-piece stainless steel ferrule (designed for use with stainless steel tubing) Stainless steel tubing Recommended tightening Finger-tight, plus 3/4-turn using wrench. 3/4-turn First use Short flats TP piece ferrule Stainless steel tubing Finger-tight, plus as much as 1/6-turn using wrench. 1/6-turn Reinstalled Short flats TP piece ferrule February 6, 2014, Rev. A 31

32 2 Preparing for Operation Installation recommendations for ASM fittings: (Continued) Fitting M-detail fitting (designed for use with large capillary tubing) Recommended tightening Finger-tight, plus 1/2-turn using a 3/16-inch open-end wrench. 1/2-turn First use Short flats Ferrule TP03497 Finger-tight, plus as much as 1/6-turn using a 3/16-inch open-end wrench. Reinstalled TP03497 Short flats Ferrule 1/6-turn 32 February 6, 2014, Rev. A

33 Making Ethernet connections Installation recommendations for ASM fittings: (Continued) Fitting V-detail fitting (designed for use with small fused silica capillary tubing) Fused silica tubing Recommended tightening Finger-tight, plus 3/4-turn using a 1/4-inch open-end wrench. 3/4-turn First use Short flats TP piece ferrule Fused silica tubing Finger-tight, plus as much as 1/6-turn using a 1/4-inch open-end wrench. Reinstalled Short flats TP piece ferrule 1/6-turn Making Ethernet connections To make connections: 1. Connect one end of one Ethernet cable to the ASM. 2. Connect the cable s other end to the network switch on the back of the µsm-fl. February 6, 2014, Rev. A 33

34 2 Preparing for Operation Making signal connections Refer to the signal connection location shown on the silk-screened label affixed to the rear panel of the ASM. Required materials 9/32-inch nut driver Flat-blade screwdriver Connector Signal cable To make signal connections: 1. Insert the connector into the connector port, on the rear panel of the ASM. Typical connector and port: Connector port Connector 34 February 6, 2014, Rev. A

35 Making signal connections 2. Using the flat-blade screwdriver, attach the positive and negative leads of the signal cable to the connector. Typical signal cable connection: Screw Connector Signal cable February 6, 2014, Rev. A 35

36 2 Preparing for Operation 3. Onto the rear panel s grounding stud, fit the grounding cable s fork terminal, and secure it with the locking nut. Tip: Use the 9/32-inch nut driver to tighten the locking nut until the fork terminal does not move. Typical grounding cable connection: Fork terminal Locking nut Grounding stud ASM I/O signal connectors The rear panel of the ASM includes two removable connectors that hold the screw terminals for I/O signal cables. These connectors are keyed so that they can be inserted only one way. 36 February 6, 2014, Rev. A

37 Making signal connections ASM I/O signal connectors: Connector I Connector II Auxiliary 1 In Auxiliary 1 In Auxiliary 2 In Auxiliary 2 In Ground Run Stopped Out Run Stopped Out Switch 1 Out Switch 1 Out Ground 0-2V Analog 1 Out 0-2V Analog 1 Out Start Gradient In Start Gradient In Stop Flow In Stop Flow In Ground Switch 2 Out Switch 2 Out Switch 3 Out Switch 3 Out Ground 0-2V Analog 2 Out 0-2V Analog 2 Out For electrical specifications, see Appendix B. ASM analog-out/event-in connections: Signal connection Auxiliary 1 In Auxiliary 2 In Run Stopped Out Switch 1 Out 0 2V Analog 1 Out Description Reserved for future use. Reserved for future use. Indicates (with a contact closure) the ASM has ceased operation because of an error condition or operator request. Used to send time-based contact closure signals to external devices. Outputs the analog signal to a device such as an integrator or strip-chart recorder. Via the MassLynx software, you can select one of the following signals as the chart out signal: Flow rate System pressure Composition (%A, %B) February 6, 2014, Rev. A 37

38 2 Preparing for Operation ASM analog-out/event-in connections: (Continued) Signal connection Gradient In Stop Flow In Switch 2 Out Switch 3 Out 0 2V Analog 2 Out Description Initiates the pumps to begin gradient operation by contact closure input or 0-volt input. Allows you to stop the flow from the ASM when an error condition or hardware failure occurs on another system module. Used to send time-based contact closure signals to external devices. Used to send time-based contact closure signals to external devices. Outputs the analog signal to a device such as an integrator or strip-chart recorder. Via the MassLynx software, you can select one of the following signals as the chart out signal: Flow rate System pressure Composition (%A, %B) Chart-out signal conditions: Signal Parameter setting at 0 volts (minimum) Parameter setting at volts (maximum) Flow Rate 0.2 μl/min Pump A: µl/min Pump B: µl/min System Pressure 345 kpa ( 3.45 bar, 50 psi) 103,421 kpa (1034 bar, 15,000 psi) Composition 0.0% 100.0% 38 February 6, 2014, Rev. A

39 Connecting to the electricity source Connecting to the electricity source Each system module requires a separate, grounded, power source. The ground connection in all power outlets must be common and physically close to the system. Warning: To avoid electric shock, observe these precautions: Use power cord SVT-type in the United States and HAR-type or better in Europe. For other countries requirements, contact your local Waters distributor. Inspect the power cord for damage and replace it, if necessary. Power-off and unplug the ASM before performing any maintenance operation on it. Connect each system module to a common ground. To connect to the electricity source: 1. Connect the female end of the power cord to the receptacle on the rear panel of the ASM. 2. Connect the male end of the power cord to a suitable wall outlet. Alternative: If your system includes the optional Waters micro-cart, ensure the ASM is powered off. Connect the female end of the cart's power supply cable (included in the Waters micro-cart startup kit) to the receptacle on the rear panel of the ASM. Preparing the ASM For optimal performance of the system, you must prepare the ASM for operation. Doing so includes these operations: Priming the seal wash Priming the ASM February 6, 2014, Rev. A 39

40 2 Preparing for Operation Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: To prevent salts from precipitating in the system, introduce an intermediate solvent, such as water, when changing from buffers to high-organic-content solvents. Requirements: To maintain the efficiency of the ASM and obtain accurate, reproducible chromatograms, use only MS-grade (or better) quality solvents and additives. Waters laboratories use, to good effect, Fisher Optima solvents. Flush the system with the appropriate solvents before allowing eluent into the column, optical detector, or mass spectrometer. The flow control module supports only the solvents (and percentage of each) shown in the Solvent drop-down list of the ASM s instrument method dialog box. Other solvents can adversely affect flow rate accuracy. Do not operate the system using mobile phases of ph greater than 10. Alkaline solutions, such as ammonium hydroxide (ph 11), can react with glass, etching solvent bottles and silica capillary tubing. The etching causes an elevated chemical background that a mass spectrometer can detect. Degassing blended solvents can change their composition. For that reason, solvents A1 and B1 bypass the degassers. Only solvents A2 and B2 undergo degassing. Priming the seal wash You prime the seal wash in the ASM to lubricate plungers, and flush away solvent or any precipitated salts that seeped past the plunger seals from the high-pressure side of the piston chambers. Prime the plunger seal wash under these conditions: Immediately after using buffered mobile phase When the ASM has been inactive for a few hours or longer 40 February 6, 2014, Rev. A

41 Preparing the ASM When the ASM is dry When troubleshooting a low-pressure error Caution: To avoid damaging the valve seats and the seals in the solvent path, do not use a nonvolatile buffer as seal wash solvent. Recommendations: Do not allow the organic-solvent component of seal wash to exceed 10%. Before priming the plunger seals, ensure the solvent reservoir contains sufficient solvent for priming and use. Required materials Tubing adapter (ASM startup kit) 30-mL syringe (ASM startup kit) Seal-wash solution Gloves: clean, powder-free, chemical-resistant To prime the seal wash: 1. Ensure the seal-wash waste line terminates in a suitable waste container. Important: Do not recycle seal wash. 2. If the system is dry, do as follows: Caution: To prevent contamination, wear clean, chemical-resistant, powder-free gloves when handling the solvent filter. Skin oils can contaminate the filter. Remove the seal-wash inlet tube from the solvent reservoir, and disconnect the inlet filter. Connect the tubing adapter to the syringe. Fill the syringe with seal-wash solution, and then connect the syringe assembly to the seal-wash inlet tube. 3. In the Console, select Auxiliary Solvent Manager from the system tree. 4. Click Control > Prime seal wash, and then click Start. February 6, 2014, Rev. A 41

42 2 Preparing for Operation 5. If you connected a priming syringe, push on the syringe plunger to force seal-wash solvent through the system. 6. When the seal-wash solvent flows from the seal-wash waste tube, click Control > Prime seal wash to stop the priming process. 7. Remove the syringe and adapter, reconnect the filter, and place the seal-wash inlet tube into the seal-wash solvent reservoir. Priming the ASM Priming, a timed operation replaces solvent in the path between the reservoirs to the vent valve, not including the flow control module. During priming, the vent valve moves to the Vent position, ensuring minimal backpressure. Prime the ASM when performing these tasks: Changing reservoirs or solvents Preparing a new system or ASM for use Operating the system after it has been idle more than four hours Caution: To prevent salts from precipitating in the system, introduce an intermediate solvent, such as water, when changing from buffers to high-organic-content solvents. Recommendations: Whenever you change solvents, purge the pump and autozero the flow control module (see Chapter 3). Ensure the solvent reservoirs contain enough solvent for adequate priming and use. The priming flow rate is 4 ml/min for each pump, or 8 ml/min total, so priming both solvents for 5 minutes requires approximately 20 ml of each solvent, for a total of 40 ml. Ensure the waste container has sufficient capacity for used solvent. To prime the ASM: 1. In the instrument console software, from the system tree, select Auxiliary Solvent Manager. 2. Click Control > Prime A/B Solvents. 42 February 6, 2014, Rev. A

43 Preparing the ASM 3. In the Prime A/B Solvents dialog box, select solvent A, solvent B, or both solvents, and then complete these selections: For solvent A, select a solvent, preferably an aqueous one. If you selected solvent B, select a solvent, preferably an organic one. 4. In the Time box, specify the duration, from 0.1 through 60.0 minutes. Recommendation: To refresh the solvent, prime the solvent manager for at least 1 minute. If you are changing from one solvent to another, prime for at least 5 minutes. 5. Click Start. Result: When the system is idle, priming is finished. 6. Repeat this procedure as needed for all solvents in use. Note: Vacuum degassing can change eluent composition at microscale flow rates. Priming a dry ASM To prime a dry ASM: 1. Open the device s front door. 2. Locate the appropriate solvent vent tube. 3. In the instrument console software, select Auxiliary Solvent Manager from the system tree. 4. Click Control > Prime A/B Solvents. 5. In the Prime A/B Solvents dialog box, select solvent A, solvent B, or both solvents, and then complete these selections: For solvent A, select a solvent. If you selected solvent B, select a solvent. 6. In the Time box, specify the duration, from 0.1 through 60.0 minutes. Recommendation: Prime the ASM until a steady flow exits the vent tube (typically between 7 to 10 minutes). 7. Click Start. 8. When you see no bubbles in the solvent stream flowing from the vent tube, repeat step 3 through step 7, to prime solvent A2, B1, and B2. February 6, 2014, Rev. A 43

44 2 Preparing for Operation Tip: Confirm that solvent reservoirs contain enough solvent for future methods. To prime a dry ASM using a syringe: 1. Open the instrument s front door. 2. Locate the appropriate solvent vent tube: If you are priming solvent A, follow the stainless steel vent tube labeled A-VENT from port 4 on the vent valve. Turn the vent tube retainer clockwise, and lift the vent tube from the drip tray. If you are priming solvent B, follow the stainless steel vent tube labeled B-VENT from port 1 on the vent valve. Turn the vent tube retainer clockwise, and lift the vent tube from the drip tray. Vent tube retainer Solvent vent tubes Drip tray 3. Push the syringe plunger fully into the syringe barrel. 4. Connect the tubing adapter to the syringe. 5. Connect the syringe assembly to the short length of PharMed tubing, and then connect the short length of PharMed tubing to the solvent vent tube you located in step In the Console, select Auxiliary Solvent Manager from the system tree. 7. In the ASM information window, click Control > Prime A/B Solvents. 8. In the Prime A/B Solvents dialog box, select solvent A1. 44 February 6, 2014, Rev. A

45 Preparing the ASM 9. In the Time box, specify a duration, in minutes, for the priming operation, from 0.1 through Recommendation: Prime the ASM until a steady flow exits the vent tube (typically 5 minutes). 10. Click Start. 11. Slowly withdraw the syringe plunger, pulling solvent through the solvent path. 12. When you see no bubbles in the solvent stream flowing from the vent tube, remove the syringe from the vent tube, and reconnect the vent tube to the drip tray. 13. Repeat step 2 through step 12 for solvent A2, B1, and B2. Tip: Ensure solvent reservoirs contain enough solvent for future methods. February 6, 2014, Rev. A 45

46 2 Preparing for Operation 46 February 6, 2014, Rev. A

47 Auto zeroing the flow transducers 3 Using the Auxiliary Solvent Manager Before using the ASM, you must auto zero the pump B flow transducer and check for leaks. Contents: Topic Page Auto zeroing the flow transducers Inspecting for leaks Auto zeroing the flow transducers Use the auto zero procedure to set the internal calibrations of the ASM s pump B flow transducer to zero flow. Recommendation: If you observe a measured flow value exceeding ±10 nl/min at a stop-flow condition, auto zero the pump B flow transducer. In the instrument console software, the measured flow rates appear in the plot-data channel Measured Flow Rate B of the ASM. Caution: To avoid damaging the outlet of the ASM s flow control module, use only a pin plug to stopper it. If the outlet of ASM s flow control module is connected to the system, do not disconnect it. If the pump B outlet is not connected to the system, however, you must stopper it using a pin plug (included in the startup kit). February 6, 2014, Rev. A 47

48 3 Using the Auxiliary Solvent Manager Pin plug in the pump B outlet of the flow control module: Flow control module Pin plug For instructions explaining how to perform the auto zero procedure, see the online Help. Inspecting for leaks For instructions about evaluating the fluid-handling integrity of the pump and system, see the online Help. 48 February 6, 2014, Rev. A

49 Maintaining the ASM 4 Maintenance Procedures Keep to a maintenance schedule, and perform maintenance operations as required and described in this chapter. Contents: Topic Page Maintaining the ASM Maintenance schedule Spare parts Maintenance considerations Troubleshooting with Connections INSIGHT Replacing solvent reservoir filters Cleaning the air filter in the door Replacing the air filter in the door Replacing the solvent filters Replacing the vent valve cartridge Replacing the i 2 Valve actuator Replacing the i 2 Valve cartridge Replacing the in-line filter cartridge on the i 2 Valve actuator Replacing the accumulator check valve Replacing the primary head plunger and seals Replacing the accumulator head plunger and seals Cleaning the exterior of the ASM Maintaining the ASM Perform the procedures in this section when you discover that a solvent manager component malfunctioned or during routine maintenance. For information about isolating such problems, consult the Console online Help. February 6, 2014, Rev. A 49

50 4 Maintenance Procedures Contacting Waters technical service If you are located in the USA or Canada, report malfunctions or other problems to Waters Technical Service ( ). From elsewhere, phone the Waters corporate headquarters in Milford, Massachusetts (USA), or contact your local Waters subsidiary. The Waters web site includes phone numbers and addresses for Waters locations worldwide. Visit When you contact Waters, be prepared to provide this information: Error message (if any) Nature of the symptom Serial number of the system module and its firmware version, if applicable Flow rate Operating pressure Solvent(s) Detector settings (sensitivity and wavelength) Type and serial number of column(s) Sample type and diluent Data software version and serial number Waters workstation model and operating system version For complete information on reporting shipping damages and submitting claims, see Waters Licenses, Warranties, and Support Services documentation. Locating system serial numbers The serial number on the system s instruments and devices facilitates service and support. Serial numbers also provide a way to create single log entries for each module, so that you can review the usage history of a particular module. Be prepared to provide the serial numbers of the instruments or devices in your system when you contact Waters customer support. To view the information for a system module: 1. In the Console, select a module from the system tree. 2. Click Configure > View module information. 50 February 6, 2014, Rev. A

51 Maintenance schedule Result: The Module Information dialog box displays this information: Serial number Firmware version Firmware checksum Component software version Alternatives: From the main window, place the pointer over the visual representation of the system module for which you want to view information. Obtain the serial number from the printed labels affixed to the rear panels of system modules or inside their front doors. Maintenance schedule Perform the following routine maintenance on the solvent manager to ensure reliable operation and accurate results. When using the system throughout the day (and on nights and weekends), or when using aggressive solvents, such as buffers, perform the maintenance tasks more frequently. Recommended routine maintenance schedule: Maintenance procedure Frequency For information... Replace solvent reservoir filters During scheduled routine maintenance or as needed See page 57 Clean the air filter in the door As needed See page 56 Replace the air filter in the door Replace the solvent filters Replace the vent valve cartridge Replace the i 2 Valve actuator During scheduled routine maintenance or as needed During scheduled routine maintenance or as needed See page 56 See page 57 As needed See page 59 5 years from the date of manufacture or as needed See page 62 February 6, 2014, Rev. A 51

52 4 Maintenance Procedures Recommended routine maintenance schedule: (Continued) Maintenance procedure Frequency For information... Replace the i 2 Valve cartridge Replace the in-line filter on the i 2 Valve cartridge Replace the accumulator check valve Replace the plunger and seals Clean the device s exterior with a soft, lint-free cloth, or paper dampened with water During scheduled routine maintenance or as needed During scheduled routine maintenance or as needed During scheduled routine maintenance or as needed During scheduled routine maintenance or as needed See page 73 See page 82 See page 86 See page 89 and page 110 As needed See page 126 Spare parts To ensure that your system operates as designed, use only Waters Quality Parts. Visit for information about Waters Quality Parts, including how to order them. Maintenance considerations Safety and handling Bear in mind these advisories when you perform maintenance operations on the system. 52 February 6, 2014, Rev. A

53 Troubleshooting with Connections INSIGHT Warning: To avoid personal contact with instrument components contaminated with biohazards or toxic materials, wear clean, chemical-resistant, powder-free gloves when reinstalling the fittings. Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Warning: To avoid electric shock, do not remove the device s protective panels. The components within are not user-serviceable. Caution: To avoid damaging the electronic components or circuitry of a system module, do not disconnect an electrical assembly from the module while the module remains connected to the ac supply source. Follow this procedure to completely interrupt power to the module: 1. Set the module s power switch to Off. 2. Disconnect the module s electrical supply cord from the ac source. Afterward, wait 10 seconds before disconnecting any assembly from the module. See Appendix A for safety advisory information. Configuring maintenance warnings Maintenance counters provide real-time usage status information that can help you determine when to schedule routine maintenance for specific components. You can set usage thresholds and maintenance warnings that alert you when a component reaches the designated threshold limit. By setting threshold limits and monitoring these usage counters regularly, you can minimize unexpected failures and unscheduled downtime during important work. For information on setting maintenance warnings, consult the Console online Help. Troubleshooting with Connections INSIGHT Connections INSIGHT is an intelligent device management (IDM) Web service that enables Waters to provide proactive service and support for the February 6, 2014, Rev. A 53

54 4 Maintenance Procedures system. To use Connections INSIGHT, you must install its service agent software on your MassLynx workstation. In a client/server system, you must also install the service agent on the computer from which you control the system. The service agent software automatically and securely captures and sends information about the support needs of your system directly to Waters. If you encounter a performance issue when using the instrument console software, you can manually submit a Connections INSIGHT request to Waters customer support. Alternatively, you can use Remote Desktop, a real-time collaboration option that controls the two-way connection with the system by enabling the Connections INSIGHT iassist service level. Consult these sources for more information about Connections INSIGHT and Connections INSIGHT iassist: Connections INSIGHT Installation Guide (part number ) Connections INSIGHT User's Guide (part number ) Your sales representative Your local Waters subsidiary Waters Customer Support To submit a Connections INSIGHT request: 1. Select Troubleshoot > Connections INSIGHT. 2. In the Connections INSIGHT Request dialog box, type your name, telephone number, address, and a description of the problem. 3. Click Submit, and allow approximately 5 minutes to save the service profile. Result: A.zip file containing your Connections INSIGHT profile is forwarded to Waters customer support for review. Tip: Saving a service profile or plot file from the instrument console software can require as much as 150 MB of file space. 54 February 6, 2014, Rev. A

55 Replacing solvent reservoir filters Replacing solvent reservoir filters Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: To avoid contaminating the solvent filter with skin oils, wear clean, chemical-resistant, powder-free gloves when handling it. Required materials Gloves: clean, powder-free, chemical-resistant New solvent filter To replace a solvent reservoir filter: 1. Remove the filtered end of the solvent tubing from the solvent bottle. 2. Remove the old solvent filter from the solvent tubing. 3. Insert the new solvent filter into the solvent tubing. Solvent filter Solvent tubing 4. Insert the filtered end of the solvent tubing into the solvent bottle. 5. Shake the filter to remove any air from it. 6. Prime the solvent manager (see page 42). February 6, 2014, Rev. A 55

56 4 Maintenance Procedures Cleaning the air filter in the door Required materials Mild detergent and water To clean the air filter: 1. Lift the air filter, removing it from the frame. Frame Air filter 2. Using a mild detergent and water clean the filter and then dry it. 3. Align the cleaned air filter within the air filter frame. Ensure the bottom of the air filter contacts the bottom of the filter frame. Replacing the air filter in the door If you cannot clean the air filter by washing, replace it with a new filter. Required material ASM air filter 56 February 6, 2014, Rev. A

57 Replacing the solvent filters To replace the air filter: 1. Remove the old air filter from the air filter frame, and discard it. Frame Air filter 2. Align the new air filter within the air filter frame. Replacing the solvent filters Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the filter. Required materials Gloves: clean, powder-free, chemical-resistant Solvent filter(s) Required tools 1/4-inch open-end wrench 5/8-inch open-end wrench February 6, 2014, Rev. A 57

58 4 Maintenance Procedures To replace a solvent filter: 1. Flush the solvent manager with nonhazardous solvent. 2. Stop the solvent flow. 3. Using the 5/8-inch open-end wrench to hold the solvent filter in place, disconnect the outlet compression fitting by using the 1/4-inch open-end wrench. Place 5/8-inch open-end wrench here Place 1/4-inch open-end wrench here Outlet compression fitting Solvent filter 4. Using the 5/8-inch open-end wrench to hold the solvent filter, disconnect the inlet compression fitting by using the 1/4-inch wrench. Place 1/4-inch open-end wrench here Place 5/8-inch open-end wrench here Inlet compression fitting Solvent filter 58 February 6, 2014, Rev. A

59 Replacing the vent valve cartridge 5. Remove the old solvent filter from the clamp. 6. Unpack the new solvent filter. 7. Insert the new solvent filter into the clamp. 8. Reattach the compression fittings to the solvent filter and tighten them finger-tight plus as much as 1/6-turn, for existing fittings, or 3/4-turn for new fittings. See also: Refer to page 29 for additional information about tightening fittings. 9. If you are replacing both solvent filters, repeat step 3 through step Perform the set pressure diagnostic test (see the online Help). Replacing the vent valve cartridge Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the vent valve cartridge. Required materials Gloves: clean, powder-free, chemical-resistant Vent valve cartridge Required tools 1/4-inch open-end wrench 2-mm Allen wrench (system startup kit) To replace the vent valve cartridge: 1. In the Console, select Auxiliary Solvent Manager from the system tree. 2. In the solvent manager information window, click Interactive Display. February 6, 2014, Rev. A 59

60 4 Maintenance Procedures 3. In the solvent manager interactive display dialog box, click Control. 4. Ensure the vent valve is set to Vent. Tip: To change the setting to Vent, click the underlined vent valve position, and select Vent. Interactive display showing vent valve setting: 5. Use the 1/4-inch wrench to remove the fittings attached to the vent valve cartridge. Vent valve cartridge: Hex screw hole at 10 o clock position Pump A to system From pump A (transducer, accumulator) Pump A vent/waste 21 Pump B vent/waste From pump B (transducer, accumulator) Pump B to system 6. Use the 2-mm Allen wrench to remove the hex screw at the 10 o clock position on the vent valve cartridge. 7. Remove the vent valve cartridge from the vent valve assembly by pulling straight forward. 8. Unpack the replacement vent valve cartridge. 60 February 6, 2014, Rev. A

61 Replacing the vent valve cartridge Caution: To avoid scratching the drive clamp or cartridge housing, use care when aligning the grooves. 9. Ensure that the groove in the cartridge housing aligns with the groove on the drive clamp. Tip: If the grooves fail to align, carefully turn the drive clamp until they do. Vent valve cartridge housing Aligned grooves Titanium material of construction identification groove Drive clamp 10. Insert the new vent valve cartridge into the vent valve cartridge chamber. Requirements: Orient the new cartridge exactly as the old one was oriented. The vent valve cartridge must slide fully into the vent valve assembly. If it does not, contact Waters Technical Service. 11. Insert the 2-mm hex screw at the 10 o clock position on the vent valve cartridge and then use the 2-mm Allen wrench to fully tighten the hex screw. 12. Use the 1/4-inch wrench to reattach all fittings and tighten them as much as 1/6-turn beyond finger-tight for existing fittings, or 3/4-turn beyond finger-tight for new fittings. 13. Prime the solvent manager (see page 42). 14. Perform the dynamic leak test and the set pressure diagnostic test (see the online Help). February 6, 2014, Rev. A 61

62 4 Maintenance Procedures Replacing the i 2 Valve actuator Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: To avoid damaging the i 2 Valve actuator, do not attempt to push or pull liquid or gas through the valve's inlet or outlet ports. To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the i 2 Valve actuator. Required materials Gloves: clean, powder-free, chemical-resistant i 2 Valve actuator i 2 Valve cartridge (recommended) Required tools 1/4-inch open-end wrench 5/16-inch open-end wrench T8 TORX driver To replace the i 2 Valve actuator: Caution: To avoid damaging a module s electrical components, do not disconnect an electrical assembly from the module when it remains connected to the electrical source. To completely interrupt power to the module, set its power switch to Off, and then unplug its electrical supply cord from the ac source. Wait 10 seconds before you disconnect an assembly. 1. Flush the solvent manager with nonhazardous solvent. 62 February 6, 2014, Rev. A

63 Replacing the i 2 Valve actuator 2. Power-off the ASM. Tip: The solvent manager is referred to as pump on the warning label affixed to the i 2 Valve actuator. Warning: To avoid injuries arising from personal contact with spilled solvent (the result of inadvertent siphoning), move the solvent bottles to a location below the solvent manager. 3. Move the solvent bottles to a location below the solvent manager. Caution: To avoid damage to the connector or cable, grasp the i 2 Valve connector by the knurled diameter. 4. Grasp the i 2 Valve connector by the knurled diameter, and pull it toward you, disconnecting it from its receptacle. Connector receptacle i 2 Valve connector Knurled diameter February 6, 2014, Rev. A 63

64 4 Maintenance Procedures 5. Loosen the cap nut on the in-line filter, removing the nut from the threads of the ferrule holder fitting. Ferrule holder fitting Cap nut 6. Use the 5/16-inch open-end wrench to loosen the shell nut, and then fully unscrew it. Place 5/16-inch open-end wrench here 64 February 6, 2014, Rev. A

65 Replacing the i 2 Valve actuator Caution: To avoid leaks, ensure the PEEK washer, which is normally on the top face of the i 2 Valve cartridge, does not remain in the head when you remove the valve assembly (see step 12 on page 77). To avoid failure of the fuse on the i 2 Valve actuator s PCB board, never place the actuator assembly or electrical connector in the drip tray. 7. Remove the i 2 Valve actuator housing from the bottom of the primary pump head. Pump head i 2 Valve actuator February 6, 2014, Rev. A 65

66 4 Maintenance Procedures 8. Remove the ferrule holder fitting from the inlet port on the old i 2 Valve actuator. Ferrule holder fitting Inlet port 9. Use the T8 TORX driver to loosen 1/2-turn the 4 screws that secure the clamping plates. 10. Ensure the shell nut remains free to rotate and that the plates slide open. Tips: Avoid touching the clamping plate tabs when loosening the screws. You can rotate the shell nut to gain access to all 4 screws. Clamping plate tab (2) Shell nut Clamping plate (2) T8 TORX screw (4) 11. When both plates are in the maximum open position, remove the cartridge from the i 2 Valve actuator. Tip: If you cannot remove the cartridge from the valve actuator, rotate the cartridge 1/2-turn, and then remove it. 66 February 6, 2014, Rev. A

67 Replacing the i 2 Valve actuator 12. Ensure the PEEK washer is inserted into the cartridge, its chamfered edge facing away from the cartridge. Chamfered edge facing away from cartridge PEEK washer i 2 Valve cartridge 13. Ensure the plates are loose and in their maximum open position. Tips: Avoid touching the clamping plate tabs when loosening the screws. You can rotate the shell nut to gain access to all 4 screws. February 6, 2014, Rev. A 67

68 4 Maintenance Procedures 14. Insert the cartridge you removed from the old i 2 Valve actuator into the new actuator, grooved end first. Recommendation: Replace the cartridge whenever you replace the i 2 Valve actuator. See step 12 on page 67. Grooved end of cartridge i 2 Valve actuator 15. With one hand, squeeze the 2 clamping plate tabs on the i 2 Valve actuator, to hold the clamping plates against the cartridge. Requirement: The clamping plates must be fully engaged in the cartridge groove. Clamping plate tab Clamping plate tab Clamping plates 68 February 6, 2014, Rev. A

69 Replacing the i 2 Valve actuator 16. While squeezing the clamping plate tabs, use the T8 TORX driver to tighten the 4 screws that secure the plates, repeating the torquing pattern shown below at least 3 times and gradually increasing the torque until the screws are uniformly tight. Start here Tip: You can rotate the shell nut to gain access to all 4 screws. Clamping plate tab (2) Shell nut Clamping plate (2) T8 TORX screw (4) February 6, 2014, Rev. A 69

70 4 Maintenance Procedures 17. Insert the ferrule holder fitting into the inlet port on the i 2 Valve assembly, and tighten it finger-tight. Ferrule holder fitting Inlet port 18. Orient the i 2 Valve assembly so that the cable exits from the left-hand side. 19. Insert the i 2 Valve assembly into the bottom of the primary pump head, and route the cable behind the valve actuator. 20. Finger-tighten the shell nut, rotating it approximately 5 full turns, to secure the valve. 70 February 6, 2014, Rev. A

71 Replacing the i 2 Valve actuator 21. Use the 5/16-inch open-end wrench to tighten the nut an additional 1/8-turn. Place 5/16-inch open-end wrench here 22. Reinsert the in-line filter and tube into the ferrule holder fitting. Ferrule holder fitting In-line filter Cap nut February 6, 2014, Rev. A 71

72 4 Maintenance Procedures 23. Place the cap nut over the ferrule holder fitting and finger-tighten the cap nut to the extent possible. Ferrule holder fitting Cap nut 24. Align the white arrow on the i 2 Valve connector with the white arrow on the receptacle, in the 12 o clock position, and insert the connector into the receptacle. Connector receptacle i 2 Valve connector Knurled diameter 25. Power-on the solvent manager. 26. Prime the solvent manager (see page 42). 27. Perform the dynamic leak test (see the online Help). 72 February 6, 2014, Rev. A

73 Replacing the i 2 Valve cartridge Replacing the i 2 Valve cartridge Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: To avoid damaging the i 2 Valve actuator, do not attempt to push or pull liquid or gas through the valve's inlet or outlet ports. To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the i 2 Valve cartridge. Required materials Gloves: clean, powder-free, chemical-resistant i 2 Valve cartridge Required tools 1/4-inch open-end wrench 5/16-inch open-end wrench T8 TORX driver To replace the i 2 Valve cartridge: 1. Flush the solvent manager with nonhazardous solvent. Caution: To avoid damaging a module s electrical components, do not disconnect an electrical assembly from the module when it remains connected to the electrical source. To completely interrupt power to the module, set its power switch to Off, and then unplug its electrical supply cord from the ac source. Wait 10 seconds before you disconnect an assembly. 2. Power-off the ASM. February 6, 2014, Rev. A 73

74 4 Maintenance Procedures Tip: The solvent manager is referred to as pump on the warning label affixed to the i 2 Valve actuator. Warning: To avoid injuries arising from contact with spilled solvent (the result of unintentional siphoning), move the solvent bottles to a location below the solvent manager. 3. Move the solvent bottles to a location below the solvent manager. Caution: To avoid damaging the connector or cable, grasp the i 2 Valve connector by the knurled diameter. 4. Grasp the i 2 Valve connector by the knurled diameter, and pull it toward you, disconnecting it from its receptacle. Connector receptacle i 2 Valve connector Knurled diameter 74 February 6, 2014, Rev. A

75 Replacing the i 2 Valve cartridge 5. Loosen the cap nut on the in-line filter, removing it from the threads of the ferrule holder fitting. Ferrule holder fitting Cap nut 6. Use the 5/16-inch open-end wrench to loosen the shell nut, and then fully unscrew it. Place 5/16-inch open-end wrench here February 6, 2014, Rev. A 75

76 4 Maintenance Procedures Caution: To avoid leaks, ensure the PEEK washer, which is normally on the top face of the i 2 Valve cartridge, does not remain in the head when you remove the valve assembly (see step 12 on page 77). To avoid failure of the fuse on the i 2 Valve actuator s PCB board, never place the actuator assembly or electrical connector in the drip tray. 7. Remove the i 2 Valve actuator from the bottom of the primary pump head. Pump head i 2 Valve actuator Caution: To avoid damaging the i 2 Valve actuator, do not back the screws out all the way. 8. Use the T8 TORX driver to loosen 1/2-turn the 4 screws that secure the clamping plates. 9. Ensure the shell nut remains free to rotate and that the plates slide open. 76 February 6, 2014, Rev. A

77 Replacing the i 2 Valve cartridge Tips: Avoid touching the clamping plate tabs when loosening the screws. You can rotate the shell nut to gain access to all 4 screws. Clamping plate tab (2) Shell nut Clamping plate (2) T8 TORX screw (4) 10. When both plates are in the maximum open position, remove the cartridge from the i 2 Valve actuator. Tip: If you cannot remove the cartridge from the valve actuator, rotate the cartridge 1/2-turn, and then remove it. 11. Unpack the new cartridge. 12. Ensure the PEEK washer is inserted into the cartridge, its chamfered edge facing away from the cartridge. Chamfered edge facing away from cartridge PEEK washer i 2 Valve cartridge February 6, 2014, Rev. A 77

78 4 Maintenance Procedures 13. With the clamping plates still open, insert the cartridge into the i 2 Valve actuator, grooved end first. Grooved end of cartridge i 2 Valve actuator 14. With one hand, squeeze the 2 clamping plate tabs on the i 2 Valve actuator, to hold the clamping plates against the cartridge. Requirement: The clamping plates must be fully engaged in the cartridge groove. Clamping plate tab (2) Clamping plates 78 February 6, 2014, Rev. A

79 Replacing the i 2 Valve cartridge 15. While squeezing the clamping plate tabs, use the T8 TORX driver to tighten the 4 screws that secure the plates, repeating the torquing pattern shown below at least 3 times and gradually increasing the torque until the screws are uniformly tight. Start here Tip: You can rotate the shell nut to gain access to all 4 screws. Clamping plate tab (2) Shell nut Clamping plate (2) T8 TORX screw (4) 16. Orient the i 2 Valve assembly so that the cable exits from the left-hand side. 17. Insert the i 2 Valve assembly into the bottom of the primary pump head, and route the cable behind the valve actuator. 18. Finger-tighten the shell nut, rotating it approximately 5 full turns, to secure the valve. February 6, 2014, Rev. A 79

80 4 Maintenance Procedures 19. Use the 5/16-inch open-end wrench to tighten the nut an additional 1/8-turn. Place 5/16-inch open-end wrench here 20. Reinsert the in-line filter and tube into the ferrule holder fitting. Ferrule holder fitting In-line filter Cap nut 80 February 6, 2014, Rev. A

81 Replacing the i 2 Valve cartridge 21. Place the cap nut over the ferrule holder fitting and finger-tighten the cap nut to the extent possible. Ferrule holder fitting Cap nut 22. Align the white arrow on the i 2 Valve connector with the white arrow on the receptacle, in the 12 o clock position, and insert the connector into the receptacle. Connector receptacle i 2 Valve connector Knurled diameter 23. Power-on the solvent manager. 24. Prime the solvent manager (see page 42). 25. Perform the dynamic leak test (see the online Help). February 6, 2014, Rev. A 81

82 4 Maintenance Procedures Replacing the in-line filter cartridge on the i 2 Valve actuator. Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: Wear clean, chemical-resistant, powder-free gloves when handling the in-line filter cartridge. Oil from your hands can contaminate the in-line filter cartridge. Required materials Gloves: clean, powder-free, chemical-resistant In-line filter cartridge Warning: To avoid injuries arising from contact with spilled solvent (the result of unintentional siphoning), move the solvent bottles to a location below the solvent manager. To replace the in-line filter: Caution: To avoid damaging the i 2 Valve actuator, do not attempt to push or pull liquid or gas through the valve's inlet or outlet ports. 1. Flush the solvent manager with nonhazardous solvent. 2. Power-off the ASM. 3. Move the solvent bottles to a location below the solvent manager. 82 February 6, 2014, Rev. A

83 Replacing the in-line filter cartridge on the i 2 Valve actuator 4. Unscrew the cap nut on the in-line filter assembly. Ferrule holder fitting Cap nut i 2 Valve actuator 5. Pull the cap nut off the tube to remove the in-line filter cartridge. Cap nut Tube In-line filter cartridge In-line filter cartridge and cap nut: In-line filter cartridge Cap nut Tube Metal locking ring 6. Put the cap nut over the end of the tube (see the figure on page 84). February 6, 2014, Rev. A 83

84 4 Maintenance Procedures 7. Slide the metal locking ring onto the tube, ensuring that the thicker end of the metal locking ring is facing toward the cap nut. Metal locking ring orientation toward cap nut: Thicker end of locking ring Cap nut Tube Thinner end of locking ring 8. Slide the in-line filter cartridge onto the tube. Exploded view of in-line filter: Ferrule holder fitting In-line filter cartridge Cap nut Metal locking ring 84 February 6, 2014, Rev. A

85 Replacing the in-line filter cartridge on the i 2 Valve actuator 9. Insert the in-line filter cartridge with tubing into the ferrule holder fitting. Ferrule holder fitting In-line filter Cap nut 10. Screw the cap nut onto the ferrule holder fitting, ensuring that the tube is bottomed out in the in-line filter cartridge. Finger-tighten it to the extent possible. 11. Return the solvent bottles to their original location. 12. Power-on the solvent manager. 13. Prime the solvent manager (see page 42). 14. Perform the dynamic leak test (see the online Help). February 6, 2014, Rev. A 85

86 4 Maintenance Procedures Replacing the accumulator check valve Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the check valve. Required materials Accumulator check-valve assembly Gloves: clean, powder-free, chemical-resistant Required tools 1/2-inch open-end wrench 1/4-inch open-end wrench 5/16-inch open-end wrench To replace the accumulator check valve: 1. Flush the solvent manager with nonhazardous solvent. Caution: To avoid damaging a module s electrical components, do not disconnect an electrical assembly from the module when it remains connected to the electrical source. To completely interrupt power to the module, set its power switch to Off, and then unplug its electrical supply cord from the ac source. Wait 10 seconds before you disconnect an assembly. 2. Power-off the ASM. Warning: To avoid injuries arising from contact with spilled solvent (the result of unintentional siphoning), move the solvent bottles to a location below the solvent manager. 3. Move the solvent bottles to a location below the solvent manager. 86 February 6, 2014, Rev. A

87 Replacing the accumulator check valve 4. Using the 5/16-inch open-end wrench to hold the check valve in place, disconnect the compression fitting by using the 1/4-inch open-end wrench. Accumulator check valve Place 5/16-inch open-end wrench here Compression fitting Caution: To avoid leaks, ensure the PEEK washer, which is normally on the top face of the accumulator check valve, does not remain in the head when you remove the valve assembly (see step 7 on page 88). 5. Use the 1/2-inch open-end wrench to loosen the check valve, and then remove the check-valve assembly from the head. Place 1/2-inch open-end wrench here February 6, 2014, Rev. A 87

88 4 Maintenance Procedures 6. Unpack the new check valve. 7. Ensure the new PEEK washer is inserted into the new check valve, its chamfered edge facing away from the check valve. Chamfered edge facing away from check valve PEEK washer Check valve Check valve housing 1/2-inch Hex nut 5/16-inch wrench flat 8. Insert the check-valve assembly into the head, and use the 1/2-inch wrench to tighten the check-valve nut 1/8-turn beyond finger-tight. 9. Finger-tighten the compression fitting and ferrule, and then, using the 5/16-inch open-end wrench to hold the check valve in place, use the 1/4-inch wrench to tighten the compression fitting as much as 1/6-turn beyond finger-tight for an existing stainless steel tubing assembly, or 3/4-turn beyond finger-tight for a new stainless steel tubing assembly. 10. Return the solvent bottles to their original location. 11. Power-on the solvent manager. 12. Prime the solvent manager (see page 42). 13. Perform the dynamic leak test (see the online Help). 88 February 6, 2014, Rev. A

89 Replacing the primary head plunger and seals Replacing the primary head plunger and seals See the online Help to help determine whether you need to replace the primary plunger seals. Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when removing and replacing the plunger seals. Required materials Can of compressed air Gloves: clean, powder-free, chemical-resistant Methanol Plunger (recommended) Plunger seal and plunger seal spacer Fluoropolymer O-ring Seal-wash seal Required tools 1/4-inch open-end wrench 5/16-inch open-end wrench Pliers Plunger removal tool (recommended) Sharp tool T27 TORX driver (startup kit) To remove the primary head: 1. Flush the solvent manager with nonhazardous solvent. February 6, 2014, Rev. A 89

90 4 Maintenance Procedures 2. In the Console, select Auxiliary Solvent Manager from the system tree. 3. In the solvent manager information window, click Maintain > Heads. 4. In the Head Maintenance dialog box, select the primary head (A or B). 5. Click Move Backward, and then wait for the plunger to stop. Caution: To avoid damaging a module s electrical components, do not disconnect an electrical assembly from the module when it remains connected to the electrical source. To completely interrupt power to the module, set its power switch to Off, and then unplug its electrical supply cord from the ac source. Wait 10 seconds before you disconnect an assembly. 6. Power-off the ASM. Tip: The solvent manager is referred to as a pump on the warning label affixed to the i 2 Valve actuator. Warning: To avoid injuries arising from contact with spilled solvent (the result of unintentional siphoning), move the solvent bottles to a location below the solvent manager. 7. Move the solvent bottles to a location below the solvent manager. 90 February 6, 2014, Rev. A

91 Replacing the primary head plunger and seals Caution: To avoid damaging the connector or cable, grasp the i 2 Valve connector by the knurled diameter. 8. Grasp the i 2 Valve connector by the knurled diameter and pull it toward you, disconnecting it from the receptacle. Connector receptacle i 2 Valve connector Knurled diameter 9. Loosen the cap nut on the in-line filter so that it is removed from the threads of the ferrule holder fitting. Ferrule holder fitting Cap nut February 6, 2014, Rev. A 91

92 4 Maintenance Procedures 10. Use the 5/16-inch open-end wrench to loosen the shell nut, and then fully unscrew it. Place 5/16-inch open-end wrench here 92 February 6, 2014, Rev. A

93 Replacing the primary head plunger and seals Caution: To avoid leaks, ensure the PEEK washer, which is normally on the top face of the i 2 Valve cartridge, does not remain in the head when you remove the valve assembly (see step 12 on page 77). To avoid failure of the fuse on the i 2 Valve actuator s PCB board, never place the actuator assembly or electrical connector in the drip tray. 11. Remove the i 2 Valve actuator from the bottom of the primary pump head, ensuring that the PEEK washer remains in the cartridge. Pump head i 2 Valve actuator February 6, 2014, Rev. A 93

94 4 Maintenance Procedures 12. Remove the seal-wash tubing secured to the seal-wash housing by barbed fittings by using a tool or by pulling on the tubing as close to the head as possible. Seal-wash tubing Seal-wash tubing 13. Using a pliers, remove the drip wire from the head assembly. Pliers Drip wire 94 February 6, 2014, Rev. A

95 Replacing the primary head plunger and seals 14. Using the 1/4-inch open-end wrench, disconnect the outlet tubing from the transducer. Transducer Outlet tubing 15. Disconnect the pressure transducer cable from the bulkhead by squeezing on the tabs and pulling gently. Pressure transducer cable connector Tabs 16. Using the T27 TORX driver, loosen the 2 head-bolts 1/2-turn. Tip: The bolts are accessible from the front of the pressure transducer. Head-bolt Head-bolt February 6, 2014, Rev. A 95

96 4 Maintenance Procedures Caution: To avoid damaging the plunger, support the pump head from below as you remove it. 17. Using the T27 TORX driver, loosen and remove the 2 support plate bolts, and then gently pull the head and support plate off the actuator housing, making sure not to tilt the head during the extraction. Support plate bolt Support plate bolt Pulling the head and support plate off the actuator housing: Plunger Head and support plate 96 February 6, 2014, Rev. A

97 Replacing the primary head plunger and seals Warning: To avoid hand lacerations, use care when removing the old plunger. Bending the plunger shaft can cause it to break. 18. Use the recessed side of the plunger removal tool to apply pressure to both sides of the release collar, and then remove the old plunger. Flat end of plunger removal tool Recessed lip of plunger removal tool Spring-loaded release collar Plunger Plunger removal tool 19. Remove the plunger removal tool from the release collar. To remove the primary plunger and seals: 1. Stand the head upright on a clean surface. 2. Using the T27 TORX driver, completely loosen the 2 head-bolts to release the support plate from the pump head. February 6, 2014, Rev. A 97

98 4 Maintenance Procedures Requirement: If you remove the transducer and head-bolts, be sure to reuse the head-bolt washers when reassembling the pump head. Head-bolt access hole Head-bolt access hole 3. Lift the pump head from the support plate. Pump head Seal-wash housing Support plate 98 February 6, 2014, Rev. A

99 Replacing the primary head plunger and seals 4. Lift the seal-wash housing from the support plate. Seal-wash housing Support plate Plunger seals: Head Fluoropolymer O-ring Seal-wash housing Plunger seal Seal-wash seal Plunger seal spacer 5. Gently pull the seal-wash seal from the seal-wash housing. Seal-wash seal February 6, 2014, Rev. A 99

100 4 Maintenance Procedures 6. Carefully withdraw the plunger seal and spacer from the seal wash housing. Plunger seal Caution: To avoid scratching any metal surfaces when removing the fluoropolymer O-ring, use a sharp tool that is softer than the metal surface. 7. Taking care not to scratch any surfaces, use a sharp tool to remove the fluoropolymer O-ring from the pump head. Fluoropolymer O-ring 8. Inspect the seal-wash housing surface and pump head surface, ensuring both are free from scratches and particulates. 9. Lubricate the new fluoropolymer O-ring with methanol, and press the O-ring into its seat in the pump head with your gloved thumb. 100 February 6, 2014, Rev. A

101 Replacing the primary head plunger and seals 10. Lubricate the new plunger seal spacer with methanol, and gently press it in the seal-wash housing with your gloved thumb. Plunger seal spacer Seal-wash housing 11. Spray the new plunger seal with the can of compressed air to remove any particulates. Important: To avoid contaminating the new plunger seal with particulates, oil, or water, do not spray the seal with unfiltered compressed air from which oil and condensate are not removed. 12. Lubricate the new plunger seal with methanol, and place it in the seal-wash housing, over the plunger seal spacer. Plunger seal Plunger seal spacer Seal-wash housing February 6, 2014, Rev. A 101

102 4 Maintenance Procedures 13. Orient the seal-wash housing so that the holes on its side align with the holes on the side of the pump head, and then guide it into place. Seal-wash housing Pump head 14. Spray the new seal-wash seal with the can of compressed air to remove any particulates. Important: To avoid contaminating the new seal-wash seal with particulates, oil, or water, do not spray the seal with unfiltered compressed air from which oil and condensate are not removed. 102 February 6, 2014, Rev. A

103 Replacing the primary head plunger and seals 15. Lubricate the new seal-wash seal with methanol, place it in the seal-wash housing, and press it into place. Seal-wash seal Seal-wash housing 16. Place the support plate on top of the pump head, ensuring the round side of the plate is oriented toward the bottom side of the head. Round side of support plate Bottom side of pump head February 6, 2014, Rev. A 103

104 4 Maintenance Procedures 17. Holding the assembly together, use the T27 TORX driver to minimally tighten the 2 head-bolts. T27 TORX driver Head-bolt access hole Head-bolt access hole To reattach the primary head: 1. Flip the assembly over, and then lubricate the seals with methanol. Seals 2. Carefully insert the sapphire plunger shaft into the pump head until the plunger shaft is no longer visible, ensuring the shaft does not contact the support plate. 104 February 6, 2014, Rev. A

105 Replacing the primary head plunger and seals Recommendation: Replace the plunger whenever you replace the plunger seal. Plunger shaft Support plate Caution: To avoid damaging the plunger, ensure that the head assembly is not tilted relative to the actuator housing when you position it on the mechanism. 3. Carefully slide the head assembly and sapphire plunger into the actuator housing, making sure not to tilt the head. 4. Hold the head assembly securely against the actuator housing, and then use the T27 TORX driver to tighten the support plate bolts securely. Support plate bolt Pump head-bolt Pump head-bolt Support plate bolt February 6, 2014, Rev. A 105

106 4 Maintenance Procedures 5. Connect the pressure transducer cable to the bulkhead. Pressure transducer cable connector 6. Power-on the solvent manager. 7. In the Console, select Auxiliary Solvent Manager from the system tree. 8. In the solvent manager information window, click Maintain > Heads. 9. In the Head Maintenance dialog box, select the primary head (A or B). 10. Click Move Forward, and then wait for the piston to engage the plunger sphere. Caution: To avoid damaging the plunger, alternately tighten the support plate screws 1/4-turn so that they are uniformly torqued. 11. Use the torque driver to tighten each pump head-bolt to 60 inch-pounds of torque. 12. After tightening each pump head-bolt to 60 inch-pounds of torque, use the torque driver to tighten each pump head-bolt to 80 inch-pounds of torque. 13. After tightening each pump head-bolt to 80 inch-pounds of torque, use the torque driver to tighten each pump head-bolt to 98 inch-pounds of torque. Caution: To avoid pinching the drip wire between the head assembly and support plate, be sure to install the drip wire after tightening the head-bolts. 14. Reinstall the drip wire around the head assembly ensuring that the tip is in the 6 o clock position. 15. Power-off the ASM. 16. Reattach all fittings and seal-wash tubing. 106 February 6, 2014, Rev. A

107 Replacing the primary head plunger and seals Tip: When reattaching the outlet tubing to the transducer, tighten the inlet-tubing fitting finger-tight plus as much as 1/6-turn, for existing fittings, or 3/4-turn for new fittings. 17. Orient the i 2 Valve assembly so that the cable exits from the left-hand side. 18. Insert the i 2 Valve assembly into the bottom of the primary pump head, and route the cable behind the valve actuator. 19. Finger-tighten the shell nut, rotating it approximately 5 full turns, to secure the valve. 20. Use the 5/16-inch open-end wrench to tighten the nut an additional 1/8-turn. Place 5/16-inch open-end wrench here February 6, 2014, Rev. A 107

108 4 Maintenance Procedures 21. Reinsert the in-line filter and tube into the ferrule holder fitting. Ferrule holder fitting In-line filter 22. Place the cap nut over the ferrule holder fitting, and finger-tighten the cap nut to the extent possible. Ferrule holder fitting Cap nut 108 February 6, 2014, Rev. A

109 Replacing the primary head plunger and seals 23. Align the white arrow on the i 2 Valve connector with the white arrow on the receptacle, in the 12 o clock position, and insert the connector into the receptacle. Connector receptacle i 2 Valve connector Knurled diameter 24. Return the solvent bottles to their original location. 25. Power-on the solvent manager. 26. Prime the solvent manager (see page 42). 27. Perform the dynamic leak test (see the online Help). If the leak test results are not satisfactory, pressurize the head plunger seals to properly seat them. To pressurize the seals: Run the solvent manager at 96,527 kpa (965 bar, 14,000 psi) for a half-hour, or run the leak test until results are satisfactory. February 6, 2014, Rev. A 109

110 4 Maintenance Procedures Replacing the accumulator head plunger and seals Consult the Console online Help to help determine whether you need to replace the accumulator plunger seals. Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when removing and replacing the plunger seals. Required materials Can of compressed air Gloves: clean, powder-free, chemical-resistant Methanol Plunger (recommended) Plunger seal and plunger seal spacer Fluoropolymer O-ring Seal-wash seal Required tools 1/4-inch open-end wrench 5/16-inch open-end wrench Pliers Plunger removal tool (recommended) Sharp tool T27 TORX driver (startup kit) 110 February 6, 2014, Rev. A

111 Replacing the accumulator head plunger and seals To remove the accumulator head: 1. Flush the solvent manager with nonhazardous solvent. 2. In the Console, select Auxiliary Solvent Manager from the system tree. 3. In the solvent manager information window, click Maintain > Heads. 4. In the Head Maintenance dialog box, select the accumulator head (A or B). 5. Click Move Backward, and then wait for the plunger to stop. Caution: To avoid damaging a module s electrical components, do not disconnect an electrical assembly from the module when it remains connected to the electrical source. To completely interrupt power to the module, set its power switch to Off, and then unplug its electrical supply cord from the ac source. Wait 10 seconds before you disconnect an assembly. 6. Power-off the ASM. Warning: To avoid injuries arising from contact with spilled solvent (the result of unintentional siphoning), move the solvent bottles to a location below the solvent manager. 7. Move the solvent bottles to a location below the solvent manager. February 6, 2014, Rev. A 111

112 4 Maintenance Procedures 8. Remove the seal-wash tubing, secured to the seal-wash housing by barbed fittings, by using a tool or by pulling on the tubing as close to the head as possible. Seal-wash tubing Seal-wash tubing 9. Using a pliers, remove the drip wire from the head assembly. Pliers Drip wire 112 February 6, 2014, Rev. A

113 Replacing the accumulator head plunger and seals 10. Using the 1/4-inch open-end wrench, disconnect the outlet tubing from the transducer. Transducer Outlet tubing 11. Using the 5/16-inch open-end wrench to hold the check-valve cartridge in place, disconnect the tubing connection from the check valve using the 1/4-inch open-end wrench. Accumulator check valve Compression fitting Place 5/16-inch open-end wrench here February 6, 2014, Rev. A 113

114 4 Maintenance Procedures 12. Disconnect the pressure transducer cable from the bulkhead by squeezing on the tabs and pulling gently. Pressure transducer cable connector Tabs 13. Using the T27 TORX driver, loosen the 2 head-bolts 1/2-turn. Tip: The bolts are accessible from the front of the pressure transducer. Head-bolt Head-bolt Caution: To avoid damaging the plunger, support the pump head from below as you remove it. 14. Using the T27 TORX driver, loosen and remove the 2 support plate bolts, and then gently pull the head and support plate off the actuator housing. 114 February 6, 2014, Rev. A

115 Replacing the accumulator head plunger and seals Important: Do not tilt the head when removing it from the actuator housing. Support plate bolt Support plate bolt Plunger Head and support plate February 6, 2014, Rev. A 115

116 4 Maintenance Procedures Warning: To avoid hand lacerations, use care when removing the old plunger. Bending the plunger shaft can cause it to break. 15. Use the recessed side of the plunger removal tool to apply pressure to both sides of the release collar, and then remove the old plunger. Flat end of plunger removal tool Recessed lip of plunger removal tool Spring-loaded release collar Plunger Plunger removal tool 16. Remove the plunger removal tool from the release collar. To remove the accumulator plunger and seals: 1. Stand the head upright on a clean surface. 2. Using the T27 TORX driver, completely loosen the 2 head-bolts to release the support plate from the pump head. 116 February 6, 2014, Rev. A

117 Replacing the accumulator head plunger and seals Requirement: If you remove the transducer and head-bolts, be sure to reuse the head-bolt washers when reassembling the pump head. Head-bolt access hole Head-bolt access hole 3. Lift the pump head from the support plate. Pump head Seal-wash housing Support plate February 6, 2014, Rev. A 117

118 4 Maintenance Procedures 4. Lift the seal-wash housing from the support plate. Seal-wash housing Support plate Plunger seals: Head Fluoropolymer O-ring Seal-wash housing Plunger seal Seal-wash seal Plunger seal spacer 5. Gently pull the seal-wash seal from the seal-wash housing. Seal-wash seal 118 February 6, 2014, Rev. A

119 Replacing the accumulator head plunger and seals 6. Carefully withdraw the plunger seal and spacer from the seal wash housing. Plunger seal Caution: To avoid scratching any metal surfaces when removing the fluoropolymer O-ring, use a sharp tool that is softer than the metal surface. 7. Use a sharp tool to remove the fluoropolymer O-ring from the pump head. Fluoropolymer O-ring 8. Lubricate the new fluoropolymer O-ring with methanol, and press the O-ring into its seat in the pump head with your gloved thumb. February 6, 2014, Rev. A 119

120 4 Maintenance Procedures 9. Lubricate the new plunger seal spacer with methanol, and gently press it in the seal-wash housing with your gloved thumb. Plunger seal spacer Seal-wash housing 10. Spray the new plunger seal with the can of compressed air to remove any particulates. Important: To avoid contaminating the new plunger seal with particulates, oil, or water, do not spray the seal with unfiltered compressed air from which oil and condensate are not removed. 11. Lubricate the new plunger seal with methanol, and place it in the seal-wash housing, over the plunger seal spacer. Plunger seal Plunger seal spacer Seal-wash housing 120 February 6, 2014, Rev. A

121 Replacing the accumulator head plunger and seals 12. Orient the seal-wash housing so that the holes on its side align with the holes on the side of the pump head, and then guide it into place. Seal-wash housing Pump head 13. Spray the new seal-wash seal with the can of compressed air to remove any particulates. Important: To avoid contaminating the new seal-wash seal with particulates, oil, or water, do not spray the seal with unfiltered compressed air from which oil and condensate are not removed. February 6, 2014, Rev. A 121

122 4 Maintenance Procedures 14. Lubricate the new seal-wash seal with methanol, place it in the seal-wash housing, and press it into place. Seal-wash seal Seal-wash housing 15. Place the support plate on top of the pump head, ensuring the round side of the plate is oriented toward the bottom side of the head. Round side of support plate Bottom side of pump head 122 February 6, 2014, Rev. A

123 Replacing the accumulator head plunger and seals 16. Holding the assembly together, use the T27 TORX driver to minimally tighten the 2 head-bolts. T27 TORX driver Head-bolt access hole Head-bolt access hole To reattach the accumulator head: 1. Flip the assembly over, and then lubricate the seals with methanol. Seals 2. Carefully insert the sapphire plunger shaft into the pump head until the plunger shaft is no longer visible, ensuring the shaft does not contact the support plate. February 6, 2014, Rev. A 123

124 4 Maintenance Procedures Recommendation: Replace the plunger whenever you replace the plunger seal. Plunger shaft Support plate Caution: To avoid damaging the plunger, ensure that the head assembly is not tilted relative to the actuator housing when you position it on the mechanism. 3. Carefully slide the head assembly and sapphire plunger into the actuator housing, making sure not to tilt the head. 4. Hold the head assembly securely against the actuator housing, and then use the T27 TORX driver to tighten the support plate bolts securely. Support plate bolt Pump head-bolt Pump head-bolt Support plate bolt 124 February 6, 2014, Rev. A

125 Replacing the accumulator head plunger and seals 5. Connect the pressure transducer cable to the bulkhead. Pressure transducer cable connector 6. Power-on the solvent manager. 7. In the Console, select Auxiliary Solvent Manager from the system tree. 8. In the solvent manager information window, click Maintain > Heads. 9. In the Head Maintenance dialog box, select the accumulator head (A or B). 10. Click Move Forward, and then wait for the piston to engage the plunger sphere. Caution: To avoid damaging the plunger, alternately tighten the support plate screws 1/4-turn so that they are uniformly torqued. 11. Use the torque driver to tighten each pump head-bolt to 60 inch-pounds of torque. 12. After tightening each pump head-bolt to 60 inch-pounds of torque, use the torque driver to tighten each pump head-bolt to 80 inch-pounds of torque. 13. After tightening each pump head-bolt to 80 inch-pounds of torque, use the torque driver to tighten each pump head-bolt to 98 inch-pounds of torque. Caution: To avoid pinching the drip wire between the head assembly and support plate, be sure to install the drip wire after tightening the head-bolts. 14. Reinstall the drip wire around the head assembly, ensuring that the tip is in the 6 o clock position. February 6, 2014, Rev. A 125

126 4 Maintenance Procedures 15. Reattach all fittings and seal-wash tubing. Tip: When reattaching the outlet tubing to the transducer, tighten the inlet-tubing fitting finger-tight plus as much as 1/6-turn, for existing fittings, or 3/4-turn for new fittings. 16. Return the solvent bottles to their original location. 17. Prime the solvent manager (see page 42). 18. Perform the dynamic leak test (see the online Help). If the leak test results are not satisfactory, pressurize the head plunger seals to properly seat them. To pressurize the seals: Run the solvent manager at 96,527 kpa (965 bar, 14,000 psi) for a half-hour, or run the leak test until results are satisfactory. Cleaning the exterior of the ASM Clean surfaces of the ASM using only a clean, soft, lint-free paper or clean cloth dampened with water. Warning: To avoid electric shock, observe these precautions: Disconnect the device or instrument from the electrical supply. When cleaning the surface of a device or instrument, apply water to a cloth, and then wipe the unit. Do not spray or otherwise directly apply water to any device surface. Warning: To avoid personal injury, use eye and hand protection during the cleaning process. 126 February 6, 2014, Rev. A

127 A Safety Advisories Waters instruments and devices display hazard symbols that alert you to the hidden dangers associated with a product s operation and maintenance. The symbols also appear in product manuals where they accompany statements describing the hazards and advising how to avoid them. This appendix presents the safety symbols and statements that apply to all of the products that Waters offers. Contents: Topic Page Warning symbols Caution advisory Warnings that apply to all Waters instruments and devices Electrical and handling symbols February 6, 2014, Rev. A 127

128 A Safety Advisories Warning symbols Warning symbols alert you to the risk of death, injury or seriously adverse physiological reactions associated with an instrument s use or misuse. Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices. The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device, or a component of an instrument or device. When one of these symbols appear in a manual s narrative sections or procedures, an accompanying statement identifies the applicable risk and explains how to avoid it. Warning: (General risk of danger. When this symbol appears on an instrument, consult the instrument s user documentation for important safety-related information before you use the instrument.) Warning: (Risk of burn injury from contacting hot surfaces.) Warning: (Risk of electric shock.) Warning: (Risk of fire.) Warning: (Risk of sharp-point puncture injury.) Warning: (Risk of hand crush injury.) Warning: (Risk of injury caused by moving machinery.) Warning: (Risk of exposure to ultraviolet radiation.) Warning: (Risk of contacting corrosive substances.) Warning: (Risk of exposure to a toxic substance.) Warning: (Risk of personal exposure to laser radiation.) 128 February 6, 2014, Rev. A

129 Warning symbols Warning: (Risk of exposure to biological agents that can pose a serious health threat.) Warning: (Risk of tipping.) Warning: (Risk of explosion.) Warning: (Risk of eye injury.) Specific warnings The following warnings (both symbols and text) can appear in the user manuals of particular instruments and devices and on labels affixed to them or their component parts. Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized: Wear eye protection. Extinguish all nearby flames. Do not use tubing that is, or has been, stressed or kinked. Do not expose nonmetallic tubing to incompatible compounds like tetrahydrofuran (THF) and nitric or sulfuric acids. Be aware that some compounds, like methylene chloride and dimethyl sulfoxide, can cause nonmetallic tubing to swell, significantly reducing the pressure at which the tubing can rupture. Mass spectrometer shock hazard The following warning applies to all Waters mass spectrometers. Warning: To avoid electric shock, do not remove the device s protective panels. The components within are not user-serviceable. February 6, 2014, Rev. A 129

130 A Safety Advisories The following warning applies to certain mass spectrometers when they are in Operate mode. Warning: To avoid nonlethal electric shock, ensure the mass spectrometer is in Standby mode before you touch any of its external surfaces that are marked with this high voltage warning symbol. Mass spectrometer flammable solvents warning This warning applies to mass spectrometers performing an analysis that requires the use of flammable solvents. Warning: To prevent ignition of flammable solvent vapors in the enclosed space of a mass spectrometer s ion source, ensure that nitrogen flows continuously through the source. The nitrogen supply pressure must not fall below 690 kpa (6.9 bar, 100 psi) during an analysis requiring the use of flammable solvents. Also a gas-fail device must be installed, to interrupt the flow of LC solvent should the nitrogen supply fail. Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans. Warning: To avoid infection with potentially infectious, human-sourced products, inactivated microorganisms, and other biological materials, assume that all biological fluids that you handle are infectious. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, B iosafety in Microbiological and Biomedical Laboratories (BMBL). Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the biohazard safety representative for your organization regarding the proper use and handling of infectious substances. 130 February 6, 2014, Rev. A

131 Caution advisory Biohazard and chemical hazard warning This warning applies to Waters instruments and devices that can process biohazards, corrosive materials, or toxic materials. Warning: To avoid personal contamination with biohazards, toxic materials, or corrosive materials, you must understand the hazards associated with their handling. Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Disposal of Chemicals. Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the safety representative for your organization regarding its protocols for handling such materials. Caution advisory Caution advisories appear where an instrument or device can be subject to use or misuse that can damage it or compromise a sample s integrity. The exclamation point symbol and its associated statement alert you to such risk. Caution: To avoid damaging the instrument s case, do not clean it with abrasives or solvents. February 6, 2014, Rev. A 131

132 A Safety Advisories Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment. Important: Toute modification sur cette unité n ayant pas été expressément approuvée par l autorité responsable de la conformité à la réglementation peut annuler le droit de l utilisateur à exploiter l équipement. Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen. Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all'utilizzo dell'apparecchiatura da parte dell'utente. Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo. 注意 : 未經有關法規認證部門允許對本設備進行的改變或修改, 可能會使使用者喪失操作該設備的權利 注意 : 未经有关法规认证部门明确允许对本设备进行的改变或改装, 可能会使使用者丧失操作该设备的合法性 주의 : 규정준수를책임지는당사자의명백한승인없이이장치를개조또는변경할경우, 이장치를운용할수있는사용자권한의효력을상실할수있습니다. 注意 : 規制機関から明確な承認を受けずに本装置の変更や改造を行うと 本装置のユーザーとしての承認が無効になる可能性があります 132 February 6, 2014, Rev. A

133 Warnings that apply to all Waters instruments and devices Warning: Use caution when working with any polymer tubing under pressure: Always wear eye protection when near pressurized polymer tubing. Extinguish all nearby flames. Do not use tubing that has been severely stressed or kinked. Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids. Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic tubing to swell, which greatly reduces the rupture pressure of the tubing. Attention: Manipulez les tubes en polymère sous pression avec precaution: Portez systématiquement des lunettes de protection lorsque vous vous trouvez à proximité de tubes en polymère pressurisés. Eteignez toute flamme se trouvant à proximité de l instrument. Evitez d'utiliser des tubes sévèrement déformés ou endommagés. Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane (THF) ou de l'acide sulfurique ou nitrique concentré. Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture. Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht: In der Nähe von unter Druck stehenden Polymerschläuchen stets Schutzbrille tragen. Alle offenen Flammen in der Nähe löschen. Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind. Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder konzentrierte Salpeter- oder Schwefelsäure verwenden. Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert. February 6, 2014, Rev. A 133

134 A Safety Advisories Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. Spegnere tutte le fiamme vive nell'ambiente circostante. Non utilizzare tubi eccessivamente logorati o piegati. Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico o nitrico concentrati. Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di rottura dei tubi stessi. Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión: El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos de polímero sometidos a presión. Si hubiera alguna llama las proximidades. No se debe trabajar con tubos que se hayan doblado o sometido a altas presiones. Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano (THF) o ácidos nítrico o sulfúrico concentrados. Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos. 警告 : 當在有壓力的情況下使用聚合物管線時, 小心注意以下幾點 當接近有壓力的聚合物管線時一定要戴防護眼鏡 熄滅附近所有的火焰 不要使用已經被壓癟或嚴重彎曲管線 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸 要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹, 大大降低管線的耐壓能力 134 February 6, 2014, Rev. A

135 Warnings that apply to all Waters instruments and devices 警告 : 当有压力的情况下使用管线时, 小心注意以下几点 : 当接近有压力的聚合物管线时一定要戴防护眼镜 熄灭附近所有的火焰 不要使用已经被压瘪或严重弯曲的管线 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀, 大大降低管线的耐压能力 경고 : 가압폴리머튜브로작업할경우에는주의하십시오. 가압폴리머튜브근처에서는항상보호안경을착용하십시오. 근처의화기를모두끄십시오. 심하게변형되거나꼬인튜브는사용하지마십시오. 비금속 (Nonmetallic) 튜브를테트라히드로푸란 (Tetrahydrofuran: THF) 또는농축질산또는황산과함께사용하지마십시오. 염화메틸렌 (Methylene chloride) 및디메틸술폭시드 (Dimethyl sulfoxide) 는비금속튜브를부풀려튜브의파열압력을크게감소시킬수있으므로유의하십시오. 警告 : 圧力のかかったポリマーチューブを扱うときは 注意してください 加圧されたポリマーチューブの付近では 必ず保護メガネを着用してください 近くにある火を消してください 著しく変形した または折れ曲がったチューブは使用しないでください 非金属チューブには テトラヒドロフラン (THF) や高濃度の硝酸または硫酸などを流さないでください 塩化メチレンやジメチルスルホキシドは 非金属チューブの膨張を引き起こす場合があり その場合 チューブは極めて低い圧力で破裂します February 6, 2014, Rev. A 135

136 A Safety Advisories Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: L utilisateur doit être informé que si le matériel est utilisé d une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d être défectueuses. Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwenddung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren. Attenzione: si rende noto all'utente che l'eventuale utilizzo dell'apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall'apparecchiatura. Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes. 警告 : 使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用, 那麼該設備所提供的保護將被消弱 警告 : 使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用, 那么该设备所提供的保护将被削弱 경고 : 제조업체가명시하지않은방식으로장비를사용할경우장비가제공하는보호수단이제대로작동하지않을수있다는점을사용자에게반드시인식시켜야합니다. 警告 : ユーザーは 製造元により指定されていない方法で機器を使用すると 機器が提供している保証が無効になる可能性があることに注意して下さい 136 February 6, 2014, Rev. A

137 Electrical and handling symbols Electrical and handling symbols Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse February 6, 2014, Rev. A 137