ACQUITY UPLC Column Manager - Active and Column Manager - Auxiliary

Transcription

1 ACQUITY UPLC Column Manager - Active and Column Manager - Auxiliary Overview and Maintenance Guide Revision A Copyright Waters Corporation 2016 All rights reserved

2 General information Copyright notice 2016 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER. The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com). Trademarks ACQUITY is a registered trademark of Waters Corporation. ACQUITY UPLC is a registered trademark of Waters Corporation. Empower is a registered trademark of Waters Corporation. MassLynx is a registered trademark of Waters Corporation. MP35N TM is a trademark of SPS Technologies Inc. THE SCIENCE OF WHAT'S POSSIBLE is a registered trademark of Waters Corporation. TORX is a registered trademark of Camcar Division, Textron Inc. Tygon is a registered trademark of Saint-Gobain Performance Plastics Corporation. UNIFI is a registered trademark of Waters Corporation. Waters is a registered trademark of Waters Corporation. All other trademarks or registered trademarks are the sole property of their respective owners. Customer comments Waters Technical Communications organization invites you to report any errors that you encounter in this document or to suggest ideas for otherwise improving it. Help us better Page ii

3 understand what you expect from our documentation so that we can continuously improve its accuracy and usability. We seriously consider every customer comment we receive. You can reach us at Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information Contacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide. Visit Telephone and fax From the USA or Canada, phone , or fax For other locations worldwide, phone and fax numbers appear in the Waters Web site. Conventional mail Waters Corporation Global Support Services 34 Maple Street Milford, MA USA Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical, biological, or radiological hazards (or any combination thereof). You must know the potentially hazardous effects of all substances you work with. Always follow Good Laboratory Practice (GLP), and consult your organization s standard operating procedures as well as your local requirements for safety. Safety hazard symbol notice Documentation needs to be consulted in all cases where the symbol is used to find out the nature of the potential hazard and any actions which have to be taken. Page iii

4 Considerations specific to the CM-A and CM-Aux Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Warning: To avoid combustion, do not use the column compartment with solvents whose autoignition temperatures are below 150 ºC. Warning: To avoid electric shock, do not remove protective panels from system modules. The components within are not user-serviceable. Warning: To avoid electrical shock, observe these precautions: Use SVT-type power cord in the United States and HAR-type power cord, or better, in Europe. For requirements elsewhere, contact your local Waters distributor. Inspect the power cord for damage, and replace it, if necessary. Power-off and unplug each module before performing any maintenance operation on it. Connect each module to a common ground. Warning: To avoid spinal and muscular injury, do not attempt to lift a system module without assistance. See also: For safety considerations regarding specific system modules, consult the appropriate information on the user documentation CD. FCC radiation emissions notice Changes or modifications not expressly approved by the party responsible for compliance, could void the user's authority to operate the equipment. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. Canada spectrum management emissions notice This class A digital product apparatus complies with Canadian ICES-001. Cet appareil numérique de la classe A est conforme à la norme NMB-001. Electrical power safety notice Do not position the instrument so that it is difficult to disconnect the power cord. Page iv

5 Equipment misuse notice If equipment is used in a manner not specified by its manufacturer, protections against personal injury inherent in the equipment s design can be rendered ineffective. Safety advisories Consult the "Safety advisories" appendix in this publication for a comprehensive list of warning advisories and notices. Operating this device When operating this device, follow standard quality-control (QC) procedures and the guidelines presented in this section. Applicable symbols Symbol Definition Manufacturer Date of manufacture Authorized representative of the European Community Confirms that a manufactured product complies with all applicable European Community directives Australia EMC compliant or Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements Consult instructions for use Alternating current Page v

6 Symbol Definition Electrical and electronic equipment with this symbol may contain hazardous substances and should not be disposed of as general waste. For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions. Serial number REF Part number catalog number Audience and purpose This guide is intended for individuals who install, operate, or maintain the column manager with the active preheater (CM-A) and auxiliary column manager (CM-Aux). It gives an overview of the technology and operation of the CM-A and CM-Aux. Intended use of the CM-A and CM-Aux Waters designed the CM-A and CM-Aux for use in liquid chromatography applications. The CM-A and CM-Aux are not intended for use in diagnostic applications. Calibrating To calibrate LC systems, adopt acceptable calibration methods using at least five standards to generate a standard curve. The concentration range for standards must include the entire range of QC samples, typical specimens, and atypical specimens. Quality control Routinely run three QC samples that represent subnormal, normal, and above-normal levels of a compound. If sample trays are the same or very similar, vary the location of the QC samples in the trays. Ensure that QC sample results fall within an acceptable range, and evaluate precision from day to day and run to run. Data collected when QC samples are out of range might not be valid. Do not report these data until you are certain that the instrument performs satisfactorily. Page vi

7 EMC considerations Canada spectrum management emissions notice This class A digital product apparatus complies with Canadian ICES-001. Cet appareil numérique de la classe A est conforme à la norme NMB-001. ISM classification: ISM group 1 class B This classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and Medical (ISM) instrument requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radiofrequency energy that is necessary for the internal functioning of the equipment. Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network. EC authorized representative Address Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX UK Telephone Fax Contact Quality manager Page vii

8 Table of contents General information...ii Copyright notice... ii Trademarks... ii Customer comments... ii Contacting Waters...iii Safety considerations...iii Safety hazard symbol notice...iii Considerations specific to the CM-A and CM-Aux... iv FCC radiation emissions notice... iv Canada spectrum management emissions notice... iv Electrical power safety notice... iv Equipment misuse notice... v Safety advisories... v Operating this device... v Applicable symbols... v Audience and purpose... vi Intended use of the CM-A and CM-Aux... vi Calibrating... vi Quality control... vi EMC considerations...vii Canada spectrum management emissions notice...vii ISM classification: ISM group 1 class B...vii EC authorized representative...vii 1 Overview Major components Column manager Column manager operation APH operation Column-switching valves ecord technology Column configurations...19 Page viii

9 1.4 Column manager system stack configurations Preparation and operation Column compartment installation Stacking system modules without interlocking features Repositioning CM-A and CM-Aux modules in the system stack Waste management Installing the leak sensor Column compartment plumbing connections Installation recommendations for fittings Preparation Connecting the tubing Connecting the column ecord fobs Column compartment power and electronic connections Making rear-panel electronic connections Connecting to a cart's electricity source Connecting to a wall electricity source Monitoring the CM-A and CM-Aux LEDs Column compartment configuration and control Specifying the column position settings Advanced mode Specifying the preheater settings Enabling the leak sensor Controlling the column compartment Responding to a leak-sensor alarm Maintenance Contacting Waters Technical Service Recommended maintenance schedule Recommended CM-A and CM-Aux routine maintenance schedule Spare parts Safety and handling Configuring maintenance warnings Replacing the column manager's leak sensor...83 Page ix

10 3.7 Replacing the ferrule on the column-inlet fitting Replacing the column manager switching-valve cartridge Removing a column Replacing the APH assembly Cleaning the exterior of the equipment...95 A Safety advisories...96 A.1 Warning symbols...96 A.1.1 Specific warnings...97 A.2 Notices...98 A.3 Bottles Prohibited symbol...98 A.4 Required protection...99 A.5 Warnings that apply to all Waters instruments and devices...99 A.6 Warnings that address the replacing of fuses A.7 Electrical symbols A.8 Handling symbols B Specifications B.1 CM-A and CM-Aux physical specifications B.2 CM-A and CM-Aux environmental specifications B.3 CM-A and CM-Aux electrical specifications B.4 CM-A and CM-Aux input/output specifications B.5 CM-A and CM-Aux performance specifications B.6 CM-A and CM-Aux wetted materials of construction C External valve installation and configuration C.1 Physical arrangement C.2 Connecting an external valve Page x

11 C.3 Configuring an external valve C.4 Controlling an external valve via method settings Page xi

12 1 Overview The Column Manager - Active (CM-A) and Column Manager - Auxiliary (CM-Aux) are columncompartment modules that manage and maintain the column temperature for ACQUITY UPLC systems. When the system includes both CM-A and CM-Aux modules, the CM-A module acts as master to as many as two CM-Aux modules. The CM-A module is fitted with two column-switching valves for column-switching applications, as well as two-dimensional applications. For two-dimensional applications, see the ACQUITY UPLC Systems with 2D Technology Capabilities Guide. The CM-Aux module has no switching valves. All plumbing connections are made to the switching valves in the CM-A module. Both CM-A and CM-Aux modules are equipped with active pre-heater (APH) devices that can heat incoming solvent before it enters the column. Additionally, the modules each have two independently controlled heating troughs (or heating zones) that can regulate the temperature of as many as four columns, two per trough in the CM-A, and one or two columns, one per trough in the CM-Aux. The CM-A and CM-Aux modules can accommodate columns with inside diameters as large as 4.6 mm and lengths up to 150 mm. For additional information about supported columns, see Column configurations. Typical column manager system configurations range from a single-stack layout with a single CM-A, to several dual-stack and triple-stack layouts with a CM-A and one or two CM-Aux modules. In the following example of a dual-stack, four-column configuration, the CM-A and CM- Aux modules are positioned above the detector and to the right-hand side of the solvent manager and sample manager. Page 12

13 Figure 1 1: Example of a CM-A with an upper CM-Aux in an ACQUITY UPLC system, dualstack configuration CM-Aux module CM-A module Detector Solvent manager Sample manager For additional information about supported column manager configurations, see Column manager system stack configurations. 1.1 Major components The following figures show the major components of the CM-A and the CM-Aux. Page 13

14 Figure 1 2: CM-A front view, with door closed On/off switch Powers the module on and off Power LED - Indicates the power-on or power-off status of the module. This LED is green when power is on and unlit when power is off. Run LED - Indicates the run status. A steady green run LED indicates that the column compartment is actively controlling the column temperature. Door Page 14

15 Figure 1 3: CM-A front view, with door open and compartment cover removed ecord receptacles Column-heating troughs Contains the column with optional column in-line filter or guard column and active pre-heater (APH) assemblies. Provides two, column-heating zones per module where column-compartment temperatures are controlled and maintained according to the settings specified in the chromatography data software. Drip tray with leak sensor Captures any leakage from the column or column connections and routes it from right to left within the compartment into the drip management system. Left-hand and right-hand APH-control ports used for connecting the APH assembly, and for directing the flow within the column-heating trough. Additionally, each control port is fitted with a retainer clip that secures the APH assembly in the control port. Column-switching valves Enables programmable switching access to any column within the CM-A, CM-Aux, or external, 30-cm column compartment that is plumbed to the columnswitching valves, as well as to column-bypass mode and to waste. Page 15

16 Figure 1 4: CM-Aux front view, with door closed Power LED - Indicates the power-on or power-off status of the module. This LED is green when power is on and unlit when power is off. The CM-Aux module's power state is controlled by the master CM-A module. Run LED - Indicates the run status. A steady green run LED indicates that the column compartment is actively controlling the column temperature. Door Figure 1 5: CM-Aux front view, with door open and compartment cover removed Column-heating troughs Contains the column with optional column in-line filter or guard column and active pre-heater (APH) assemblies. Provides two, column-heating zones per module where column-compartment temperatures are controlled and maintained according to the settings specified in the chromatography data software. Location of two, ecord receptacles on right-hand side of CM-Aux module chassis Page 16

17 Column-compartment waste collection and drain tube Captures any leakage from the column or column connections and routes it into the drip management system. Left-hand and right-hand APH-control ports used for connecting the APH assembly, and for directing the flow within the column-heating trough, Additionally, each control port is fitted with a retainer clip that secures the APH assembly in the control port. 1.2 Column manager Each CM-A and CM-Aux module consists of two independently heated and cooled column troughs. You can set the column compartment temperature from 4.0 to 90.0 ºC, in 0.1 ºC increments. The compartment controllable temperatures range from 25 ºC below ambient to 90.0 ºC. Four ecord receptacles within the CM-A module, and two ecord receptacles on the side of the CM-Aux module, receive the column's ecord fob, if one is present. The ecord chip within the fob stores column manufacturing and usage data that is accessible from the console. For more information about the recorded column data, see ecord technology. Both the CM-A and the CM-Aux modules have their own power supply. Although the CM-Aux modules have their own power supply, the CM-A controls their power state Column manager operation The column manager module controls the ambient air temperature around the column as well as the solvent before it enters the column. When the temperature is set, either directly from the console or within a method, a command is sent to the column manager to switch the compartment heating or cooling element on or off. On the basis of feedback from the compartment thermistor, the heating or cooling element continues to heat or cool until the compartment achieves the specified temperature set point. The column-heating troughs are enclosed by the compartment cover and the door, to insulate the column from changes in ambient temperature. Recommendation: When sample and column temperature are important to an application, in addition to specifying explicit temperature set points in the method, specify appropriate temperature limits. Together, these settings ensure that system operation occurs only within the defined limits and that any occurrence of an unacceptable deviation from the set points is flagged by an error message acknowledging the variance APH operation The standard, APH assembly provides thermal equilibration by heating the incoming mobile phase and injected sample to the specified column temperature before it enters the column. The assembly uses an electric heating element to directly heat the column-inlet tubing. The rapid Page 17

18 heating, low volume design reduces gradient delay and extra-column band-spreading. It ensures that high-flow rate separations occur at the desired column temperature, not at the ambient temperature of the incoming solvent. By actively controlling the temperature of the mobile phase at the point of use the column inlet sensitivity to ambient temperature swings is greatly reduced. When pre-heating is unnecessary, the APH functionality can be disabled via the console or the method editor in the chromatography data software. Figure 1 6: Typical APH assembly Protective cap covering tubing and ferrule Gold, dual-threaded fitting with locking cap nut for connecting to column or column in-line filter APH circuit board Strain-relief sleeve APH-inlet tubing SST sleeve Long, flat compression screw fitting for connecting to sample-manager injector Protective cap covering tubing and ferrule Column-switching valves The CM-A is fitted with two, internal, 9-port column-switching valves and offers a bypass channel and automated, programmable switching between columns for method development. To manage the column inlet and outlet connections, one of the column-switching valves is designated as the inlet-switching valve and the other valve as the outlet-switching valve. If necessary, you can exchange the inlet-switching valve and outlet-switching valve cartridges to accommodate different column configurations or system stack layouts. For additional information about column manager configurations, see Column manager system stack configurations. Page 18

19 1.2.4 ecord technology ACQUITY UPLC columns include an ecord column chip that tracks the usage history of the column. The ecord column chip interacts with the system software, recording information for as many as 50 sample queues run on the column. In regulated environments, the ecord column chip provides documentation of the column used in the validation method. The ecord column chip provides documentation of the column used for each chromatographic run and records the following information: The name of the sample set (or sample list) run on the column. Number of injections onto the column. Number of samples injected onto the column. The highest pressure that the column has experienced (and the date). The highest temperature the column has experienced (and the date). In addition to the variable column usage data, the ecord column chip also stores fixed column manufacturing data, including: unique column identification. certificate of analysis. QC test data. When you attach the column's ecord fob to the receptacle on the column compartment, the chip automatically records and stores system information. You need take no further action. 1.3 Column configurations You can configure the CM-A module for use with two columns (in any combination of 50-mm, 100-mm, or 150-mm columns), or with as many as four, 50-mm columns. To accommodate additional 100-mm or 150-mm columns, the ACQUITY UPLC system can include one or two CM- Aux modules (for a maximum of six columns per system). Note: Columns are not provided with the column manager. Table 1 1: Supported column configurations for CM-A and CM-Aux Quantity Description 2 length of column = 150 mm, maximum; column ID = 4.6 mm, maximum; column guard or column in-line filter = 20 mm, maximum 4 (CM-A only) length of column = 50 mm; column ID = 4.6 mm, maximum; no column guard or column in-line filter For H-Class and H-Class Bio systems, these column configurations are supported: Page 19

20 2 columns, using one CM-A 4 columns, using a CM-Aux stacked above a CM-A 4 columns, using one CM-A (fitted with 50-mm columns only) 6 columns, using one CM-A stacked between two CM-Aux For I-Class systems, only two columns, using one CM-A is supported. 1.4 Column manager system stack configurations To reduce dead volume, and ensure optimal performance, you must plumb all column manager configurations using pre-configured tubing lengths. Therefore, the modules must occupy specific positions in the system stack. One CM-Aux can rest atop the CM-A, and the CM-A can rest atop a second CM-Aux. Additionally, the placement of the inlet-switching valve in the column compartment corresponds to the arrangement of the sample manager and detector modules in the system stack. When the detectors are positioned above, or to the left of the sample manager, the inlet-switching valve is installed on the right-hand side. When the detectors are positioned to the right of the sample manager in dual-stack or triple-stack layouts, the inlet-switching valve is installed on the left-hand side. The following figure shows examples of various, 2-column, 4-column, and 6-column CM-A and CM-Aux configurations in single-stack, dual-stack, and triple-stack system layouts. For additional information on how to arrange the modules in an ACQUITY H-Class or I-Class system stack, consult your Waters technical service representative. Note: For ACQUITY UPLC I-Class systems, the only supported column manager configuration is a CM-A module configured with one or two columns. Page 20

21 Figure 1 7: Examples of typical column manager system stack configurations Dual stack ELS PDA TUV CM-A* Single stack TUV CM-Aux CM-A* Sample manager Solvent manager CM-Aux CM-A PDA Sample manager Solvent manager Sample manager Solvent manager CM-Aux CM-A CM-Aux PDA CM-Aux CM-A CM-Aux Sample manager Solvent manager Sample manager Solvent manager TUV CM-Aux CM-A* ELS TUV CM-Aux CM-A* CM-A* - Configured with a right-hand inlet-switching valve. SQD Sample manager Solvent manager SQD Sample manager Solvent manager Triple stack ELS PDA Sample manager Solvent manager CM-Aux CM-A CM-Aux ELS PDA Sample manager Solvent manager CM-Aux CM-A CM-Aux SQD Sample manager Solvent manager PDA CM-Aux CM-A CM-Aux ELS SQD Page 21

22 2 Preparation and operation Before running samples, you must prepare the CM-A and CM-Aux for operation. To prepare the CM-A and CM-Aux for operation, you must first prepare the sample manager, solvent manager, and detectors. To prepare the CM-A and CM-Aux modules for operation, perform these tasks: Task For a new installation or a system stack reconfiguration, complete these tasks, as necessary: Determine the type of column manager configuration that you require. Install the CM-A and CM-Aux modules onto the system stack. If necessary, reposition the system modules for a dual-stack or triple-stack configuration. Verify that the waste management for the column manager modules is properly configured. Connect the column manager rear-panel control and Ethernet cabling. Connect the column manager modules to the electricity source. Power-on and monitor the column manager's operation status from the front-panel LEDs. For additional information See these sections: Column configurations and Column manager-system stack configurations Stacking system modules without interlocking features and Repositioning the CM-A and CM- Aux modules in the system stack Waste management and Installing the leak sensor Making the rear-panel electronic connections Connecting to a cart's electricity source or Connecting to a wall electricity source Monitoring the CM-A and CM-Aux LEDs Page 22

23 Task For plumbing connections, complete these tasks, as necessary: Identify the components and connections that you require to plumb the column configuration. Verify that the inlet and outlet switching-valves and the APH assemblies are correctly positioned within the column compartments for the prescribed column configuration. Make the CM-A switching-valve connections and connect the columns. Optionally, connect as many as three, external valve modules to the CM-A module. Connect the columns' ecord fobs to their designated ecord receptacles. For configuration and control of the column manager, complete these tasks, as necessary: Specify the column compartment's column position and preheater settings in the chromatography data software. Control the column manager from the console or instrument (or inlet) method in the chromatography data software. For additional information See these sections: Column configurations and Column manager-system stack configurations Identifying the plumbing components and connections. Exchanging the inlet and outlet switching-valve cartridges and Repositioning the APH assemblies Connecting the tubing External valve installation and configuration Connecting the column ecord fobs See these sections: Specify the column position settings and Specify the preheater settings Controlling the column compartment 2.1 Column compartment installation If the CM-A and CM-Aux modules are not installed as part of the system stack, do as follows: Stack the modules Set up waste management Install the leak sensor Stacking system modules without interlocking features This procedure applies to system modules that are not equipped with interlocking features. Warning: To avoid spinal and muscular injury, do not attempt to lift a system module without assistance. Page 23

24 Warning: To avoid crushing your fingers beneath or between modules, use extreme care when installing a module in the system stack. To stack the modules: 1. Align the front and rear feet of the module that you are adding with the corresponding indents in the top of the chassis of the previously added module in the system stack. Figure 2 1: Aligning feet with indents Feet on underside of module being stacked Indents on top side of previously added module 2. Carefully lower the module so that the feet rest in the indents. Important: To maintain the integrity of the system stack and integrated waste system, ensure that the feet of the upper module rest in the indents of the lower module. 3. Repeat steps 1 and 2 for the remaining system modules Repositioning CM-A and CM-Aux modules in the system stack Follow these steps when you must reposition the CM-A and CM-Aux modules in the system stack. Tip: When repositioning the modules, ensure the bottommost column module, the CM-A or the CM-Aux, is fitted with the CM-A drip tray equipped with a leak detector. For details, see Waste management. Page 24

25 Required tools and materials 1/4-inch open-end wrench For tightening or loosening stainless steel (gold-plated) fittings with 2-piece ferrule. 1/2-inch open-end wrench For tightening or loosening dual-threaded fitting with locking cap nut. Chemical-resistant, powder-free gloves Right-angle nut extender For tightening or loosening one-piece PEEK fittings used for H- Class low-pressure outlet connections, such as to detector or mass spectrometer. To reposition the modules in the system stack: 1. Power-off the column manager (CM-A) and detector. 2. Disconnect the power cables from the rear panels of the CM-A, upper CM-Aux and lower CM-Aux (if present), and detector. 3. Disconnect the Ethernet cables from the rear panel of the CM-A and detector. 4. Disconnect the power and control cables from the CM-Aux modules. Warning: To avoid injuries arising from solvent spills, remove the solvent bottles from the solvent tray before removing the solvent tray. 5. Remove all solvent bottles from the bottle tray. 6. Remove the solvent tray from the system stack. 7. Disconnect the waste tubing from the detector and the inlet tubing from the CM-A outlet. 8. Remove the detector from the system stack. 9. Remove the waste tubing that is connected to the waste port on the inlet-switching valve from the solvent manager drip tray waste port. 10. Remove the sample manager outlet tubing from port 6 of the injector. 11. Arrange the system modules in the new configuration. 12. Set up the waste management for the new configuration. Note: For instructions on setting up waste management, see Waste management. 13. Place the solvent tray in the appropriate location in the system stack. 14. Place the solvent bottles in the solvent tray. 15. Reconnect the power cables to the CM-A, upper CM-Aux and lower CM-Aux (if present), and detector. Note: For instructions on connecting the power cables, see Connecting to a cart's electricity source or Connecting to a wall electricity source. 16. Reconnect the external control cables to the rear of the CM-A and CM-Aux modules. Page 25

26 Note: For instructions on connecting the external control cables, see Making the rearpanel electronic connections. 17. Reconnect the Ethernet cables at the rear panels of the CM-A and detector Waste management When setting up waste management for CM-A and CM-Aux configurations, follow these guidelines: When stacking the CM-A atop the sample manager, the CM-A waste drips into the sample manager's upper drip tray. If desired, leak detection is afforded by a leak sensor installed in the sample manager's upper drip tray. For all other column manager configurations, leak detection requires having a leak sensor installed in the bottommost column compartment's drip tray. When stacking a CM-Aux atop the CM-A, you must connect a waste tube from the CM-Aux module's drip tray to the CM-A module's drip block. When stacking both CM-A and CM-Aux atop a detector, you must install a swivel drain and extended feet on the CM-A, and direct the column compartment waste to a suitable waste container. When stacking a CM-A between an upper and lower CM-Aux, you must perform these tasks: Install the extended feet on the lower CM-Aux. Swap drip trays between the CM-A and lower CM-Aux. Connect the convoluted drip tubing segments between the upper and lower CM-Aux's drip trays and the CM-A. Install a swivel drain and waste tubing on the lower CM-Aux. Direct the column compartment waste from the lower CM-Aux to a suitable waste container. The topics in this section convey instructions for setting up waste management to accommodate the various CM-A and CM-Aux configurations Setting up waste management for a CM-A with upper CM-Aux When stacking a CM-Aux atop the CM-A, you must connect a waste tube from the CM-Aux module's drip tray to the CM-A module's drainage block. Additionally, if you stack the CM-A atop a detector, you must also install the extended feet and swivel drain on the CM-A. For instructions on installing the extended feet and swivel drain on the lower CM-Aux, see Installing the extended feet and Installing the swivel drain and waste tubing. Required tools and materials CM-A 4-column tubing kit Page 26

27 To set up the waste management components for a CM-A with upper CM-Aux: 1. Obtain the 10.4-cm (4.125-in) convoluted drip tube from the CM-A 4-column tubing kit. 2. Install the convoluted drip tube from the drip tray of the CM-Aux to the front waste port of the drainage block within the CM-A s tubing guide assembly. Tip: To avoid leaks, ensure that the convoluted drip tube is fully inserted into the front waste port of the CM-A s drainage block. Figure 2 2: Installing the convoluted drip tube between the CM-Aux and CM-A CM-Aux Convoluted drip tube CM-A tubing guide assembly (with integrated drainage block) Drip tube inserted into front waste port in drainage block Setting up waste management for a CM-A with an upper and lower CM-Aux When stacking a CM-A between an upper and lower CM-Aux, you must perform these tasks: Install the extended feet on the lower CM-Aux. Swap drip trays between the CM-A and lower CM-Aux. Connect the convoluted drip tubing segments between the upper and lower CM-Aux's drip trays and the CM-A. Install a swivel drain and waste tubing on the lower CM-Aux. Direct the column compartment waste from the lower CM-Aux to a suitable waste container. Page 27

28 Required tools and materials CM-A 4-column tubing kit CM-A 6-column tubing kit CM-A start-up kit T20 TORX driver To set up the waste management components for a CM-A with upper and lower CM-Aux: 1. Install the extended feet on the lower CM-Aux as described in Installing the extended feet. 2. Stack the upper CM-Aux, CM-A, and lower CM-Aux modules on a bench top, to the right of the sample manager and solvent manager. Note: For details about repositioning the modules in the system stack, see Repositioning CM-A and CM-Aux modules in the system stack. Figure 2 3: CM-A and CM-Aux modules stacked beside sample manager and solvent manager Sample manager Solvent manager CM-Aux CM-A CM-Aux Requirement: To ensure adequate ventilation for the system modules, when placing the modules on the bench top, maintain a 7.6-cm (3-in) gap between the module stacks. Note: If the leak sensor is installed in the CM-A, uninstall it from the CM-A. After completing this procedure, install it in the lower CM-Aux's drip tray as described in Installing the leak sensor. 3. Use the T20 TORX driver to loosen the upper and middle screws that secure the CM-A tubing guide assembly to the module. Page 28

29 Figure 2 4: Loosening the upper, tubing guide assembly screw 1 Upper screw Figure 2 5: Loosening the middle, tubing guide assembly screw 1 Middle screw 4. Using the T20 TORX driver, remove the lower screw, which secures the CM-A s drip tray to the module. Page 29

30 Figure 2 6: Loosening the lower, tubing guide assembly screw 1 Lower screw 5. Remove the tubing guide assembly from the CM-A, separate the drip tray from the tubing guide assembly, and then set the tubing guide and drip tray aside. Figure 2 7: Separating the drip tray from the tubing guide assembly 1 2 Tubing guide assembly Drip tray 6. Repeat step 3 through step 5, to remove and separate the drip tray and tubing guide assembly for the lower CM-Aux module. 7. Insert the CM-Aux drip tray into the CM-A tubing guide assembly, and then insert the CM-A drip tray into the CM-Aux tubing guide assembly. Page 30

31 Figure 2 8: CM-A and CM-Aux tubing guide assemblies and drip trays CM-Aux tubing guide assembly CM-A drip tray CM-A tubing guide assembly CM-Aux drip tray 8. Reinstall the CM-A tubing guide assembly (with CM-Aux drip tray) into the CM-A module, and then secure it in place using the 3, T20 TORX screws. Requirement: Tighten the upper and middle screws, and replace the drip tray screw. 9. Obtain the 10.4-cm (4.125-in) convoluted drip tube from the CM-A 4-column tubing kit. 10. Install the convoluted drip tube from the drip tray of the upper CM-Aux to the front waste port of the drainage block within the CM-A's tubing guide assembly. Tip: To avoid leaks, ensure that the convoluted drip tube is fully inserted into the front waste port of the CM-A s drainage block. Page 31

32 Figure 2 9: Installing the convoluted drip tube between the CM-Aux and CM-A CM-Aux 10.4-cm (4.125-in) convoluted drip tube CM-A tubing guide assembly (with integrated drainage block) Drip tube inserted into front waste port in drainage block 11. Obtain the 5.1-cm (2-in) convoluted drip tube from the CM-A 6-column tubing kit. 12. Install the convoluted drip tube onto the CM-A drip tray. Figure 2 10: Installing the convoluted drip tube onto the CM-A drip tray CM-A Lower CM-Aux Page 32

33 CM-A drip tray 5.1-cm (2-in) convoluted drip tube 13. Insert the open end of the convoluted drip tube into the front waste port of the drainage block on the lower CM-Aux s tubing guide assembly, and carefully reinstall the tubing guide assembly with drip tray into the lower CM-Aux, as shown in the figure below. Figure 2 11: Reinstalling the drip block assembly onto the lower CM-A 1 2 Tips: CM-A drip tray Lower CM-Aux tubing guide assembly (with integrated drainage block and drip tray) Use one hand to support the drip tray. You can loosen or tighten the two, T20 TORX securing screws slightly, so that the screw heads can fit into the slots in the drip block. If the internal drip tube extends into the drainage block, use needle-nose pliers to extend the tube outward approximately 0.64 cm (0.25 in). Page 33

34 Figure 2 12: Extending the drip tube outward 1 Extending the tube approximately 0.64 cm (0.25 in). 14. Install the swivel drain and waste tubing on the lower CM-Aux as described in Installing the swivel drain and waste tubing Installing the extended feet on the bottommost column module Installation of the extended feet allows room for the swivel drain that is required when the column compartment is located atop a detector or bench top. Required tools and materials Column manager start-up kit T20 TORX driver To install the extended feet on a CM-A or a CM-Aux: 1. Place the CM-A or CM-Aux so that it is positioned on it s side. 2. Using the T20 TORX driver, remove the screws that secure the four feet to the bottom of the CM-A or CM-Aux. Page 34

35 Figure 2 13: Removing column manager feet 1 Screws (4) 3. From the CM-A start-up kit, obtain the 4 extended feet and 4 M4 25-mm screws. 4. Using the T20 TORX driver and 4 M4 25-mm screws, fasten the short and long rubber feet with washers onto the bottom of the CM-A or CM-Aux. Figure 2 14: Installing the extended feet Washer with short rubber foot (4) Long rubber foot (4) M4 25-mm screw (4) T20 TORX driver 5. Place the CM-A or CM-Aux with the extended feet on the bench, to the right of the solvent manager, and ensure that at least a 7.6-cm (3-in) space remains between the modules, for ventilation. Page 35

36 Installing the swivel drain and waste tubing To collect waste from the upper column compartments and route it to a suitable waste container, you must install a swivel drain and waste tubing onto the bottommost column module (the CM-A or CM-Aux) that is fitted with the CM-A drip tray. Required tools and materials T8 TORX driver Tubing cutter Methanol Sufficient length of 0.25-in ID Tygon tubing, for connecting the drain fitting to the waste container (included in the start-up kit) Suitable waste container Column manager start-up kit To install the swivel drain and waste tubing: 1. Push the latches atop the column compartment cover inward, to disengage them from the latch pins. 2. Tilt the column compartment cover forward, just enough to clear the valve drip tray. Figure 2 15: Slightly opened column compartment (example showing CM-A) Valve drip tray Column compartment cover Page 36

37 Latches Latch pins 3. Pull the column compartment cover up and away from the module. 4. Obtain the swivel drain kit and Tygon tubing from the CM-A start-up kit. 5. Remove the mounting screw and washer from the swivel drain cup. Figure 2 16: Swivel drain cup 1 2 Mounting screw Washer Notice: To avoid leaks, ensure the O-ring is properly seated in the swivel drain cup. 6. Install the swivel drain cup on the bottom of the drip tray, and thread the mounting screw through the hole in the top of the drip tray. Page 37

38 Figure 2 17: Threading the mounting screw through the drip tray Mounting screw and washer Opening in top of drip tray CM-A drip tray O-ring Swivel drain cup 7. Use the T8 TORX driver to minimally tighten the mounting screw. Requirement: Do not overtighten the screw. Tip: If you cannot swivel the drain cup, the mounting screw is too tight. Loosen the mounting screw until the drain cup swivels freely. 8. Using methanol, wet the barbed portion of the fitting. 9. Slide one end of the tubing over the barbed portion of the fitting. Page 38

39 Figure 2 18: Routing the waste tubing through the drain fitting 1 Drain fitting 10. Cut the opposite end of the tubing to the proper length, and then connect it to the waste container. 11. Route the tubing, ensuring that liquid can drain through it freely to the waste container. Warning: To avoid a hazardous condition resulting from leakage or spilling of waste fluid, ensure that the drain tube is properly routed so that it is not crimped or bent. A crimp or bend can prevent adequate flow to the waste container. Warning: To avoid spills, empty the waste container at regular intervals. Notice: To avoid fluid backup and false leak-sensor alarms, ensure proper drainage of waste: Place the waste container below the system stack, to permit gravity flow. Ensure that the waste and degasser vent tubes do not crimp or bend. A crimp or bend can impede flow to the waste container. Ensure that the exit of the waste and degasser vent tubes is not immersed in waste solvent. If necessary, shorten each tube so that no portion of it drops below the top of the waste container. Page 39

40 Figure 2 19: Representation of a suitable waste container Waste tubing (from stack of system modules) Waste container vent tubing (to fume hood) Waste container Installing the leak sensor For leak detection with the column manager configuration, install a leak sensor in the integrated drip tray beneath the bottommost column manager (CM-A or CM-Aux). Note: In a single-stack configuration with the CM-A positioned atop the sample manager, the CM-A module's waste drips into the sample manager's upper drip tray. Accordingly, for leak detection, install the leak sensor in the sample manager's upper drip tray. Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Page 40

41 Required tools and materials Needle-nose pliers Chemical-resistant, powder-free gloves Leak sensor assembly To install the leak sensor: 1. Power-off the column manager. 2. Carefully unpack the leak sensor. 3. Open the column compartment door. 4. In the drip tray at the bottom of the compartment, locate the slot in the leak-sensor reservoir. Figure 2 20: Locating slot in leak-sensor reservoir Slot in leak-sensor reservoir Leak sensor reservoir 5. Align the leak-sensor T-bar with the slot in the side of the leak-sensor reservoir, and slide the leak sensor into place. Figure 2 21: Aligning the leak-sensor T-bar Leak-sensor T-bar Page 41

42 Figure 2 22: Installing leak sensor in reservoir Leak-sensor installed in leak-sensor reservoir 6. Using the needle-nose pliers to hold the connector, plug the leak-sensor cable connector into the receptacle on the front of the column manager. Figure 2 23: Connecting the leak-sensor cable to the receptacle on front of column manager Leak-sensor connector receptacle 7. Tuck the leak-sensor cable below the door latch and under the column compartment. 8. Before operating the column compartment, enable the leak sensor. For instructions, see Enabling the leak sensor. 2.2 Column compartment plumbing connections For ACQUITY UPLC systems that are configured with a CM-A or CM-A and CM-Aux modules, you must plumb the column compartments for automated column switching by connecting the columns to the CM-A module's switching valves using pre-configured tubing assemblies. When making the column tubing connections, heed these warnings: Page 42

43 Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Warning: Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials. Consult the Material Safety Data Sheets regarding the solvents you use. Additionally, consult the safety representative for your organization regarding its protocols for handling such materials. Warning: To avoid personal contamination with biohazards, wear clean, chemicalresistant, powder-free gloves when performing this procedure. Warning: To avoid personal contamination with toxic materials, wear clean, chemicalresistant, powder-free gloves when performing this procedure Installation recommendations for fittings When making the column compartment plumbing connections, consult the topics in this section for detailed instructions about assembling and tightening the various fittings used to connect the column compartment to the system. These three types of tubing and fittings connect the column compartment to the system, depending on its configuration: SST (stainless steel) tubing and fittings MP35N (a nickel-cobalt-chromium-molybdenum alloy) tubing, for H-Class Bio applications PEEK (polymer-based) tubing and fittings, for low-pressure outlet connections to detectors or mass spectrometers When connecting tubing, heed the following recommendations for installing and tightening fittings. Recommendations: To prevent bandspreading, ensure that the tubing bottoms in its fitting before you tighten the compression screw. Whenever you loosen fittings during maintenance, examine them for cracks, stripped threads, and deformations. Do not reuse stainless steel fittings more than six times Assembling new fittings For metallic (SST or MP35N) fitting assemblies with ferrules not previously assembled or set to tubing, you must mark the compression screw and fitting body, and ensure that the two marks line up when you tighten them. Page 43

44 Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Required tools and materials 1/4-inch open-end wrench For tightening or loosening stainless steel (gold-plated) fittings with 2-piece ferrule. Permanent marker Chemical-resistant, powder-free gloves To assemble the new fittings: 1. Insert the end of a tube into the hexagonal end of the compression screw. 2. Insert the tube into the larger end of the ferrule. 3. Insert the tube into the fitting body. 4. Rotate the compression screw, clockwise, into the fitting body until the screw is finger-tight. 5. Using a permanent marker, mark the compression screw at the 12 o clock position. 6. Mark the fitting body at the 9 o clock position. 7. Ensure that the tubing makes contact with the bottom of the fitting body, and use the 1/4- inch open-end wrench to rotate the compression screw clockwise 3/4-turn until the two marks line up Stainless steel (gold-plated) fitting with long flats and 2-piece stainless-steel ferrule First use Long flats Compression screw 2-piece stainless-steel ferrule Tighten the fitting finger-tight plus an additional 3/4-turn using a 1/4-inch open-end wrench. For detailed instructions about assembling new fittings, see Assembling new fittings. Page 44

45 Tip: To prevent band spreading, ensure that the tubing is fully bottomed in the fitting before tightening the compression screw. First use tightening Re-installed Long flats Compression screw 2-piece stainless-steel ferrule Tighten the fitting finger-tight plus as much as an additional 1/6-turn using a 1/4-inch open-end wrench. Re-installed tightening Page 45

46 Stainless steel (gold-plated) fitting with short flats and 2-piece stainless-steel ferrule First use Short flats Compression screw 2-piece stainless steel ferrule Tighten the fitting finger-tight plus an additional 3/4-turn using a 1/4-inch open-end wrench. For detailed instructions about assembling new fittings, see Assembling new fittings. Tip: To prevent band spreading, ensure that the tubing is fully bottomed in the fitting before tightening the compression screw. First use tightening Re-installed Page 46

47 Short flats Compression screw 2-piece stainless steel ferrule Tighten the fitting finger-tight plus as much as an additional 1/6-turn using a 1/4-inch open-end wrench. Re-installed tightening Dual-threaded fitting with locking cap nut Required tools and materials 1/2-inch open-end wrench Figure 2 24: First use or re-installed Locking cap nut Gold-plated, locking compression screw Back-locking or standard PEEK ferrule Page 47

48 To tighten the fitting: 1. Loosen the cap nut from the compression screw. 2. Slide the compression screw together with the ferrule into the inlet of the column (or in-line filter). 3. Finger-tighten the compression screw into the inlet of the column (or in-line filter). Important: To prevent band spreading, ensure that the tubing bottoms in its fitting before you tighten the compression screw. 4. Engage the column (or in-line filter), and tighten the column (or in-line filter) onto the compression screw. 5. Tighten the cap nut onto the compression screw. Tip: If you are operating the system at 103,421 kpa (1034 bar, 15,000 psi) or higher, finger-tighten the cap nut onto the compression screw, and then use the 1/2-inch open-end wrench to tighten the compression screw an additional 1/8 to 1/6-turn. Tip: When reinstalling the fitting, examine the captive ferrule for damage, and replace it, if necessary; always loosen the locking cap nut before re-connecting the fitting One-piece PEEK fitting First use or re-installed Compression screw Ferrule Tighten the fitting finger-tight. Page 48

49 V-detail fitting (for use with small, fused silica capillary tubing) First use Short flats Compression screw 2-piece stainless steel ferrule To tighten the fitting: Tighten the fitting finger-tight plus an additional 3/4-turn using a 1/4-inch open-end wrench. Tip: To prevent band spreading, ensure that the tubing is fully bottomed in the fitting before tightening the compression screw. First use tightening Re-installed Page 49

50 Short flats Compression screw 2-piece stainless steel ferrule To tighten the fitting: Tighten the fitting finger-tight plus as much as an additional 1/6-turn using a 1/4-inch open-end wrench. Re-installed tightening Preparation Before you can connect the CM-A module's switching-valve tubing, you must prepare the column compartment and ensure that you have the correct column tubing and switching valves for your application Identifying the plumbing components and connections Before you connect the column manager plumbing components, ensure that you have the column tubing and switching valve kits that are required for your ACQUITY UPLC system type and column configuration. These column tubing kits are available for the CM-A: 2-column tubing kit for H-Class 2-column tubing kit for I-Class 4-column tubing kit 4-column 50mm tubing kit 6-column tubing kit The tubing kits contain the pre-configured tubing assemblies, APH assemblies, and fittings in 316 SST or MP35N, for bio configurations. The switching valve kits are 316 stainless steel or titanium, for bio configurations. Page 50

51 Important: 4-column and 6-column configurations require multiple tubing kits to provide all of the necessary tubing components. Accordingly, 4-column CM-A and CM-Aux configurations require both the 2-column and 4-column kits. 6-column CM-A and CM-Aux configurations require the 2-column, 4-column, and 6-column kits. The following table lists the features that identify standard and bio system switching valves and APH assemblies. Tip: Tubing contained in the SST or MP35N column tubing kits does not have an identification sleeve. Table 2 1: Switching valve and APH assembly identification features Component H-Class system identification features H-Class Bio system identification features I-Class system identification features 9-position switching valve Stainless steel valve cartridge, stator ID = 11 Titanium valve cartridge, stator ID = 12 Stainless steel valve cartridge, stator ID = 22 APH assembly White sleeve, labeled 316SST Blue sleeve, labeled MP35N White sleeve, labeled. 003 The following column configuration diagrams show the connection details for each tubing kit as well as the location of the APH assemblies and the inlet and outlet-switching valves. Tips: The tubing kits contain connection diagrams on magnetic labels that you can install inside the front doors of the CM-A and upper and lower CM-Aux modules and use for reference when making the switching-valve connections. The numbers identifying the ecord receptacles in the connection diagrams correlate to the valve ports used to connect the columns. As such, they indicate which ecord receptacle to use when connecting a column's ecord fob. For more information, see Connecting the column ecord fobs. The column positions referenced in the diagrams are the default positions that are specified in the console software when you configure the modules. You can change the column positions in the compartment, as necessary. When you do so, ensure that you change the corresponding column positions in the console. For instructions on specifying the column positions, see Specifying the column position settings. Page 51

52 Figure 2 25: 2-column CM-A - right inlet To detector 3 B 4 2 O W Outlet switching valve ecord receptacles 3 4 Inlet switching valve 3 B 4 2 I W CM-A Column position 1 Column position 2 APH APH To waste Sample manager 6 Injector Figure 2 26: 4-column 50 mm CM-A left inlet In Inlet switching valve ecord receptacles 4 Outlet switching valve Out APH Column position 1 Column position 2 APH CM-A APH Column position 3 Column position 4 APH Page 52

53 Figure 2 27: 4-column CM-A and CM-Aux right inlet To detector Upper CM-Aux Column position 1 Column position 2 APH APH Tub ing Router 1 ecord receptacles 2 3 B 4 2 O W Outlet switching valve ecord receptacles 3 4 Inlet switching valve 3 B 4 2 I W CM-A Column position 3 Column position 4 APH APH To waste Sample manager 6 Injector Page 53

54 Figure 2 28: 6-column CM-A and (2) CM-Aux left inlet To detector Tubing Router APH APH Column position 1 Column position 2 Upper CM-Aux 1 ecord receptacles 2 From inject valve 3 B 4 2 I W Inlet switching valve ecord receptacles 3 4 Outlet switching valve 3 B 4 2 O W APH APH Column position 3 Column position 4 CM-A To waste Tubing Router APH APH Column position 5 Column position 6 Lower CM-Aux 5 ecord receptacles 6 Table 2 2: 2-column configuration plumbing connection details From To Description Length Material CM-A inlet valve, port B CM-A outlet valve, port B Bypass tubing, in ID (0.004-in ID for I-Class) 36.8 cm (14.5 in) 316 SST CM-A inlet valve, port W Solvent manager front drip tray waste port Waste tubing, inch ID (0.004-in ID for I-Class) Cut to length 316 SST Page 54

55 Table 2 2: 2-column configuration plumbing connection details (continued) From To Description Length Material CM-A inlet valve, port 3 Column 1 inlet APH assembly with inch ID inlet tubing (0.003-inch ID inlet tubing for I-Class) 31.8 cm (12.5 in) 316 SST or MP35N (Bio) CM-A inlet valve, port 4 Column 2 inlet APH assembly with inch ID inlet tubing (0.003-inch ID inlet tubing for I-Class) 31.8 cm (12.5 in) 316 SST or MP35N (Bio) Column 1 outlet CM-A outlet valve, port 3 Pre-configured tubing assembly, inch ID (0.003-inch ID inlet tubing for I-Class) 48.3 cm (19.0 in) 316 SST or MP35N (Bio) Column 2 outlet CM-A outlet valve, port 4 Pre-configured tubing assembly, inch ID (0.003-inch ID inlet tubing for I-Class) 48.3 cm (19.0 in) 316 SST or MP35N (Bio) Sample manager injection valve, port 6 CM-A inlet valve, center port Pre-configured tubing assembly, inch ID (0.003-inch ID inlet tubing for I-Class) 36.8 cm (14.5 in) 316 SST or MP35N (Bio) Detector inlet CM-A outlet valve, center port Pre-configured tubing assembly, inch ID (0.003-inch ID inlet tubing for I-Class). Alternatively, PEEK tubing using one-piece PEEK fittings for H- Class or V-detail fitting for I- Class 36.8 cm (14.5 in), or cut to length for PEEK 316 SST, MP35N (Bio), or PEEK Table 2 3: 4-column or 6-column configuration plumbing connection details From To Description Length Material CM-A inlet valve, port B CM-A outlet valve, port B Bypass tubing, in ID 36.8 cm (14.5 in) 316 SST CM-A inlet valve, port W Solvent manager front drip tray waste port Waste tubing, inch ID Cut to length 316 SST CM-A inlet valve, port 1 (upper CM- Aux) Column 1 inlet APH assembly with inch ID inlet tubing 31.8 cm (12.5 in) 316 SST or MP35N (Bio) CM-A inlet valve, port 2 (upper CM- Aux) Column 2 inlet APH assembly with inch ID inlet tubing 31.8 cm (12.5 in) 316 SST or MP35N (Bio) Page 55

56 Table 2 3: 4-column or 6-column configuration plumbing connection details (continued) From To Description Length Material CM-A inlet valve, port 3 (CM-A) CM-A inlet valve, port 4 (CM-A) CM-A inlet valve, port 5 (lower CM- Aux) CM-A inlet valve, port 6 (lower CM- Aux) Column 1 outlet (upper CM-Aux) Column 2 outlet (upper CM-Aux) Column 3 outlet (CM-A) Column 4 outlet (CM-A) Column 5 outlet (lower CM-Aux) Column 6 outlet (lower CM-Aux) Sample manager injection valve, port 6 Detector inlet Column 3 inlet Column 4 inlet Column 5 inlet Column 6 inlet CM-A outlet valve, port 1 CM-A outlet valve, port 2 CM-A outlet valve, port 3 CM-A outlet valve, port 4 CM-A outlet valve, port 5 CM-A outlet valve, port 6 CM-A inlet valve, center port CM-A outlet valve, center port APH assembly with inch ID inlet tubing APH assembly with inch ID inlet tubing APH assembly with inch ID inlet tubing APH assembly with inch ID inlet tubing Pre-configured tubing assembly, inch ID Pre-configured tubing assembly, inch ID Pre-configured tubing assembly, inch ID Pre-configured tubing assembly, inch ID Pre-configured tubing assembly, inch ID Pre-configured tubing assembly, inch ID Pre-configured tubing assembly, inch ID (0.003-inch ID inlet tubing for I-Class) Pre-configured tubing assembly, inch ID (0.003-inch ID inlet tubing for I-Class) Alternatively, PEEK tubing with one-piece PEEK fittings for H-Class or V-detail fitting for I- Class 31.8 cm (12.5 in) 31.8 cm (12.5 in) 47 cm (18.5 in) 47 cm (18.5 in) 48.3 cm (19.0 in) 48.3 cm (19.0 in) 48.3 cm (19.0 in) 48.3 cm (19.0 in) 57 cm (22.5 in) 57 cm (22.5 in) 36.8 or 48.3 cm (14.5 or 19 in) 36.8, 48.3, or 57 cm (14.5, 19, or 22.5 in), or cut to length for PEEK 316 SST or MP35N (Bio) 316 SST or MP35N (Bio) 316 SST or MP35N (Bio) 316 SST or MP35N (Bio) 316 SST or MP35N (Bio) 316 SST or MP35N (Bio) 316 SST or MP35N (Bio) 316 SST or MP35N (Bio) 316 SST or MP35N (Bio) 316 SST or MP35N (Bio) 316 SST or MP35N (Bio) 316 SST, MP35N (Bio), or PEEK Page 56

57 Removing the existing valve fittings and column components Follow this procedure when you must reconfigure the plumbing connections made to the CM-A module's inlet and outlet switching-valves. You can also use this procedure to remove the fittings before replacing a switching valve. Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Notice: To avoid damaging the pre-heater assembly, turn the column onto the pre-heater assembly when installing or removing the column to prevent the pre-heater tubing from twisting; do not grasp and turn the pre-heater assembly; do not suspend or hang the pre-heater tubing. Required tools and materials 1/4-inch open-end wrench Right-angle nut extender For loosening one-piece PEEK fittings used by H-Class systems for low-pressure outlet connections, such as those used to connect to a detector or mass spectrometer Permanent marker For marking fittings with their valve port location Chemical-resistant, powder-free gloves To remove the valve fittings and column components from the CM-A: 1. Stop any running mobile phase. 2. After allowing sufficient time for cooling, open the column compartment door. 3. Power-off the CM-A and the detector. 4. Disconnect the power and Ethernet cables from the rear panel of the CM-A and detector. Warning: To avoid injuries arising from solvent spills, remove the solvent bottles from the solvent tray before removing the solvent tray. Page 57

58 5. If the solvent tray is positioned above the CM-A, remove all solvent bottles from the bottle tray, and then remove the solvent tray from the system stack. 6. Push the latches atop the CM-A s column compartment cover inward, to disengage them from the latch pins. Figure 2 29: Opening the column compartment cover Latch pins Valve drip tray Column compartment cover Latches 7. Tilt the column compartment cover forward, just enough to clear the valve drip tray. 8. Pull the column compartment cover up and away from the CM-A. 9. For each fitting installed on the inlet and outlet switching-valves of the CM-A that you must remove, use a pen to mark the fitting with the number of the port to which it is connected. Note: If you must change the location of the inlet-switching valve as part of your plumbing reconfiguration, you can do so before you remove any fittings by exchanging the inlet and outlet switching-valve cartridges. For instructions, see Exchanging the CM-A inlet and outlet-switching valve cartridges. 10. Using the 1/4-inch wrench, disconnect all fittings required for reconfiguration from the inlet and outlet switching-valves. 11. For each installed column that you are removing, detach the column's ecord fob from the ecord receptacle in the CM-A module. Page 58

59 12. Remove the connected column components (column, column in-line filter, and APH assembly) in their entirety from the column troughs. 13. Continue with the appropriate instructions in this section for making the required plumbing connections for your column configuration Exchanging the CM-A inlet and outlet switching-valve cartridges In a multiple stack configuration, you can place the CM-A module to the right of the sample manager. In such a configuration, you must locate the inlet switching-valve on the left-hand side of the module. To simplify the inlet and outlet-valve exchange, you can exchange the valve cartridges with the existing fittings remaining connected to the valve ports. Tip: Exchanging the switching-valve cartridges with fittings connected is optional, but doing so ensures that all the fittings originally installed on a valve are reinstalled on the same valve in the new configuration. Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Required tools and materials 2-mm hex wrench Chemical-resistant, powder-free gloves To exchange the CM-A inlet and outlet switching-valve cartridges: 1. In the console, select Column Manager from the system tree. 2. In the column manager information window, click Column selection. 3. In the Column Manager Set Column dialog box, select Waste. 4. Use the 2-mm hex wrench to remove the hex screw at the 10 o clock position on the switching-valve cartridge. Page 59

60 Figure 2 30: Switching-valve cartridge Lock ring Lock ring stop Stator ID 2-mm hex screw 5. Remove the switching-valve cartridge from the switching-valve assembly by pulling it straight outward. 6. Label the inlet switching-valve cartridge. 7. Repeat step 1 through step 7 for the outlet switching-valve cartridge. 8. For both inlet and outlet switching-valve cartridges, ensure that the groove in the cartridge housing aligns with the groove on the drive clamp. Requirement: If the grooves fail to align, turn the drive clamp until they do align, taking care to avoid scratching the drive clamp or housing. Figure 2 31: Aligning the cartridge housing and drive clamp grooves Drive clamp Aligned grooves Page 60

61 Switching-valve cartridge housing 9. Insert the inlet switching-valve cartridge into the switching-valve cartridge chamber on the opposite side of the column manager. Requirement: Ensure that the inlet cartridge is oriented in the same position as the previously installed outlet cartridge. It must slide fully into the switching-valve assembly. If it does not, contact Waters technical service. 10. Insert the 2-mm hex screw at the 10 o clock position on the switching-valve cartridge, and tighten the screw using the 2-mm hex wrench. 11. Insert the outlet switching-valve cartridge into the switching-valve cartridge chamber on the opposite side of the column manager. Requirement: Ensure that the outlet cartridge is oriented in the same position as the previously installed inlet cartridge. It must slide fully into the switching-valve assembly. If it does not, contact your Waters service representative. 12. Insert the 2-mm hex screw at the 10 o clock position on the switching-valve cartridge, and tighten the screw using the 2-mm hex wrench Repositioning the APH assembly During initial setup, or when changing column configurations, ensure that the APH retainer and the APH assembly are installed in their proper locations in the column compartment (see Identifying the plumbing components and connections). If you must later move these components, for example, to change to a column configuration that requires the APH assembly to be installed in a left-hand control port, do so as explained below. Page 61

62 Figure 2 32: APH assembly and retainer (example showing location for a right-hand control port) Right-hand control port APH retainer APH assembly Page 62

63 Figure 2 33: APH retainer and APH assembly locations in the CM-A and CM-Aux Left-hand and right-hand APH control port locations in CM-Aux Left-hand and right-hand APH control port locations in CM-A Guidelines: For a 2-column setup in the CM-A or CM-Aux, install only one APH assembly and retainer per heating trough in the left or right control port. For a 4-column 50 mm setup in the CM-A, install an APH assembly and retainer in each of the four control ports. Page 63

64 When the inlet-switching valve is installed in the left-hand side of the CM-A compartment, install the APH assembly in left control port of each column trough that you intend to use. Doing so directs the flow from left to right within the CM-A and CM-Aux compartments. When the inlet-switching valve is installed in the right-hand side of the compartment, install the APH assembly in right control port of each column trough. Doing so directs the flow from right to left within the CM-A and CM-Aux compartments. The following procedure describes how to reposition the APH assembly to a right or left control port, as necessary. Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Required tools and materials Needle-nose pliers Chemical-resistant, powder-free gloves To reposition the APH assembly to the right or left control port in the column trough: 1. Remove the APH assembly from the retainer in the control port. 2. If necessary, use the needle-nose pliers to gently compress the left and right sides of the APH retainer to remove it from the control port. 1 Gently compress the retainer at these locations to remove it from the control port. 3. Ensure that the destination control port location is fitted with an APH retainer. 4. Insert the APH assembly into its retainer that is installed in the destination control port. Page 64

65 2.2.3 Connecting the tubing Follow these steps to connect the tubing to the CM-A switching valves. Required tools and materials 1/4-inch open-end wrench For tightening or loosening stainless steel (gold-plated) fittings with 2-piece ferrule. 1/2-inch open-end wrench For tightening or loosening dual-threaded fitting with locking cap nut. Permanent marker For marking the compression screw and fitting when assembling new fittings. Chemical-resistant, powder-free gloves Stainless steel tubing cutter For cutting the waste tubing to length. Right-angle nut extender For tightening or loosening one-piece PEEK fittings used for lowpressure outlet connections to a detector or a mass spectrometer. Tubing and fittings supplied with the 2-column, 4-column, and 6-column tubing kits, depending on your CM-A column configuration. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. To connect the tubing: 1. Refer to the figures and tables in Identifying the plumbing components and connections for the specific plumbing details on your configuration. 2. Obtain the tubing and fittings required to make all of the valve connections on the inlet and outlet switching valves according to your configuration. 3. Open the column compartment doors, and remove the column compartment covers from the CM-A and CM-Aux (if present). 4. If necessary, reposition the CM-A inlet and outlet switching-valve cartridges for a right-inlet or left-inlet configuration. Note: For instructions, see Exchanging the CM-A inlet and outlet switching-valve cartridges. 5. Prepare the column manager modules for reconfiguration by removing the fittings, tubing, and column components that are currently connected to the CM-A switching valves, as necessary. Note: For instructions, see Removing the valve fittings and column components from the CM-A. Page 65

66 6. For valve connections, pull the column manager's switching-valve forward, approximately 2 cm (0.75 in), and on each valve, rotate the lock ring clockwise until it rests against the stopper. Tip: Before connecting the waste tubing and sample manager injector-valve tubing to the switching valve, do these tasks: Straighten the waste tubing, and then route it through the tubing guide and into the designated hole in the solvent manager drip tray. If necessary, use the tubing cutter to cut off excess waste tubing. Route the injector-valve tubing through the clip in the right-hand side of the column compartment and into cutout in the top of the sample manager door, and then connect the tubing to port 6 on the sample manager's injector valve. Figure 2 34: Routing the injector-valve tubing through the cutout in the sample manager door injector-valve tubing routed through top of sample manager door Notice: To avoid pinching the tubing, do not route the tubing behind the samplemanager door. 7. Using the 1/4-inch wrench, connect the tubing to the switching valves according to your configuration starting with the waste (port W located at the 3 o'clock position on the inletswitching valve) and bypass (port B): Important: Always use port W located at the 3 o'clock position on the inlet-switching valve when connecting the waste tubing to the solvent manager's drip tray. Tips: To attach tubing with new fittings, see Assembling new fittings. To attach tubing with existing fittings, use the 1/4-inch wrench to tighten the fittings 1/6 turn beyond finger tight. Page 66

67 After connecting the bypass tubing to the bypass ports, bend the bypass tubing so that it fits within the column compartment. Use a pen to coil the excess column tubing close to the switching valves so that any leakage coming from the valve connection drips into the valve drip tray. Plug all unused ports on the inlet and outlet switching valves using pin plugs. 8. When all valve connections are made, rotate the valves' lock rings counterclockwise, to the 12 o'clock position. 9. Push the switching-valve assembly inward until it stops. 10. Connect the tubing from the outlet-switching valve outlet to the detector or mass spectrometer inlet according to the instructions in the instrument's overview and maintenance guide. 11. For each column that you are connecting, repeat the remaining steps. 12. Remove the plug from the inlet of the column (or column in-line filter). 13. Loosen the cap nut from the gold fitting on the APH assembly. 14. Slide the gold fitting with ferrule into the inlet of the column (or in-line filter). Note: For instructions explaining how to install the column in-line filter, see the document ACQUITY UPLC Column In-Line Filter Unit Installation Instructions. 15. Rotate the column onto the gold fitting and finger-tighten into the inlet of the column (or inline filter). 16. Tighten the cap nut onto the gold fitting of the APH assembly. Important: If you are operating the system at 103,421 kpa (1034 bar, 15,000 psi) or higher, finger-tighten the cap nut onto the compression screw, and then use the 1/2-inch open-end wrench to tighten the compression screw an additional 1/8 to 1/6-turn. 17. Insert the column-outlet tubing (pre-configured SST assembly or PEEK tubing) into the outlet of the column. 18. While holding the column, tighten the fitting for the column outlet according to the type of fitting used. Note: For tightening instructions, see Installation recommendations for fittings. 19. Press the column-outlet fitting into the clip, and rotate the clip so that the opening faces forward. 20. Carefully install the APH assembly into the APH retainer, aligning the column (or in-line filter with column) inside the trough. 21. Attach the column's ecord fob to the designated receptacle on the inside of the CM-A or on the right-hand side of the CM-Aux. Page 67

68 Note: For details on which ecord receptacles you must use to connect your column ecord fobs, see Connecting the column ecord fobs. 22. Route the column's ecord lanyard and tubing through the column compartment's grooves in the sides, top, or bottom of the column compartment, as appropriate. Important: Before operating the column manager with active pre-heating, verify that the column preheater settings in the console are set to Active. For instructions, see Specifying the preheater settings. Figure 2 35: Location of grooves for routing tubing 1 2 Location of grooves on right-hand side and top of column compartment Location of grooves on left-hand side and bottom of column compartment Connecting the column ecord fobs To record the column ecord data, attach each column's ecord fob to the designated receptacle in the CM-A, or on the right-hand side of the CM-Aux, according to the figures below. Note: Before the ecord chip can transfer the recorded data to the console software, you must enable the ecord in the console. For instructions on enabling the ecord, see the console's online Help. Page 68

69 Figure 2 36: ecord fob locations in the CM-A For a 4-column 50-mm configuration Top left column (connected to valve port 5). For a 2-column configuration Top column (connected to valve port 3). For a 4-column 50-mm configuration Top right column (connected to valve port 3). For a 2-column configuration Bottom column (connected to valve port 4). For a 4-column 50-mm configuration Bottom right column (connected to valve port 4). For a 4-column 50-mm configuration Bottom left column (connected to valve port 6). Figure 2 37: ecord fob locations on right-hand side of CM-Aux For an upper CM-Aux configuration Top column (connected to valve port 1). For a lower CM-Aux configuration Top column (connected to valve port 5). Page 69

70 For an upper CM-Aux configuration Bottom column (connected to valve port 2). For a lower CM-Aux configuration Bottom column (connected to valve port 6). 2.3 Column compartment power and electronic connections Both the CM-A and the CM-Aux modules require their own power supply from an external ac source. Although the CM-Aux modules have their own power supply, the CM-A controls their power state when the CM-Aux is connected to the CM-A via a control cable. Communication with the chromatography data software is achieved by an Ethernet cable connection between the CM- A and the sample manager. The topics in this section convey instructions for making these power and electronic connections Making rear-panel electronic connections To operate a CM-A and CM-Aux configuration, you must connect the control cabling to the rear panel of the CM-A and CM-Aux modules. For the CM-A and CM-Aux modules to communicate with the chromatography data software, you must connect an Ethernet cable from an Ethernet port on the sample manager's rear panel to the Ethernet port on the CM-A rear panel. Note: If you are connecting external switching valves, see External valve installation and configuration for instructions on how to connect the control cable to the Com B communication port on the CM-A rear panel. Page 70

71 Figure 2 38: Example CM-A and CM-Aux configuration showing rear-panel electronic connections CM-Aux control port in upper CM-Aux Control cable connecting CM-A to upper CM-Aux Lower CM-Aux control port Control cable connecting CM-A to lower CM-Aux CM-Aux control port in lower CM-Aux Upper CM-Aux control port Ethernet port Required tools and materials Flat-blade screwdriver CM-A and CM-Aux start-up kits Page 71

72 To make the rear-panel connections: 1. If your configuration includes an upper CM-Aux, connect a control cable from the CM-A module's upper CM-Aux control port to the CM-Aux control port on the upper CM-Aux. 2. If your configuration includes a lower CM-Aux, connect a control cable from the CM-A module's lower CM-Aux control port to the CM-Aux control port on the lower CM-Aux. 3. Using the flat-head screwdriver, tighten the retaining screws to secure the control cable connectors to the receptacles on the CM-A and CM-Aux rear panel. 4. Connect an Ethernet cable from an Ethernet port on the sample manager's rear panel to the Ethernet port on the CM-A rear panel Connecting to a cart's electricity source If your system includes the optional FlexCart or micro cart, follow this procedure to connect each module to a power source. Warning: To avoid electrical shock, observe these precautions: Use SVT-type power cord in the United States and HAR-type power cord, or better, in Europe. For requirements elsewhere, contact your local Waters distributor. Inspect the power cord for damage, and replace it, if necessary. Power-off and unplug each module before performing any maintenance operation on it. Connect each module to a common ground. Recommendation: Use a line conditioner and uninterruptible power supply (UPS) for optimum, long-term, input-voltage stability. To connect to a cart's electricity source: 1. Connect the female end of the cart's electrical cables (included in the startup kit) to the receptacle on the rear panel of each system module. 2. Connect the hooded, male end of the cart's electrical cables to the power strips on its back. 3. Connect each power strip's cable to a wall outlet operating on its own circuit. Page 72

73 2.3.3 Connecting to a wall electricity source Warning: To avoid electrical shock, observe these precautions: Use SVT-type power cord in the United States and HAR-type power cord, or better, in Europe. For requirements elsewhere, contact your local Waters distributor. Inspect the power cord for damage, and replace it, if necessary. Power-off and unplug each module before performing any maintenance operation on it. Connect each module to a common ground. Recommendation: Use a line conditioner and uninterruptible power supply (UPS) for optimum, long-term, input-voltage stability. To connect to a wall electricity source: 1. Connect the female end of the power cord to the receptacle on the rear panel of the module. 2. Connect the male end of the power cord to a suitable wall outlet. 2.4 Monitoring the CM-A and CM-Aux LEDs Light emitting diodes on the CM-A and each CM-Aux indicate the instrument s state of functioning. These include the power LED and run LED. The CM-A s power LED, on the left-hand side of the front panel, is steady green to indicate that the instrument is powered on. The CM-Aux s power LED, also on the left-hand side of the front panel, is steady green after the CM-Aux is configured (in the console) and the CM-A is rebooted. The power LED is unlit after the CM-Aux is removed from the instrument configuration and the CM-A is rebooted. The run LED, on the right-hand side of the power LED on the CM-A s and CM-Aux s front panel, is steady green when the instrument with the program-selected column (either the CM-A or CM- Aux) is running a method. The run LED is unlit when the instrument is idle and functioning normally. 2.5 Column compartment configuration and control Before you can operate a column manager with active pre-heating, you must configure it in the console to ensure that the column manager is ready to operate according to your application. You control the column manager via the console or instrument method in the chromatography data software. Page 73

74 2.5.1 Specifying the column position settings To specify the column position settings: 1. Power-on the CM-A and sample manager. 2. In the instrument console, select Column Manager > Configure > Instrument. Figure 2 39: Four-column configuration Page 74

75 Figure 2 40: Six-column configuration Page 75

76 Figure 2 41: Four-column configuration using 50-mm columns 3. Enable the upper and lower CM-Aux, as necessary, and then click OK. Tip: When you enable the upper CM-Aux, the default CM-A column numbers change to 3 and In the Configuration Confirmation window, click OK, reboot the CM-A, and then click OK to restart the console. 5. From the console, click Configure > Arrange Instrument Modules, and then click and drag the instrument modules to appropriate locations, if necessary Advanced mode For 2-dimensional applications, you can directly control the CM-A's two, internal, columnswitching valves through the Advanced Mode features in the method editor. For additional details about using the advanced mode of operation, consult a Waters Technical Service representative. Page 76

77 2.5.3 Specifying the preheater settings Before operating the system, for each enabled temperature zone or column trough that has an APH assembly installed, you must specify a column preheater setting of Active, to enable active pre-heating for that temperature zone. When enabled and set to Active, active pre-heating raises the temperature of the incoming mobile phase (and injected sample) to the same temperature as that of the temperature zone. Requirement: To support active pre-heating, connect columns with APH assemblies installed in the APH control ports. To heat solvent before it enters the column, a column's APH assembly must be both connected and enabled. Additionally, before running samples, you can change the preheater setting to enable, or disable, active pre-heating, as necessary, to suit different applications. Important: If you enable active pre-heating in the console, and then remove the APH assembly from the compartment, a system alarm occurs, and the error message Column active preheater configured but not detected appears. To avoid this condition, before removing the APH assembly, change the console's column preheater setting to Active, disabled. To specify the preheater settings: 1. From the console's Column Manager instrument configuration page, select Configure > Preheater. 2. In the Preheater box, specify the Preheater setting as follows: Select Active, to enable active pre-heating. During operation, the APH heats the incoming solvent to the temperature specified for the temperature zone (or column trough) in the console or instrument method. Select Active, disabled, to disable active pre-heating. During operation, if the APH is installed (present), active pre-heating is disabled. If the APH is uninstalled (not present), this setting prevents the associated system alarm from occurring Enabling the leak sensor The location for leak detection varies according to the column manager configuration. In a singlestack configuration with the CM-A positioned atop the sample manager, the CM-A module's waste drips into the sample manager's upper drip tray. Accordingly, leak detection is afforded by the leak sensor in the sample manager's upper drip tray. For instructions on how to enable the leak sensor for a leak sensor installed in the sample manager's upper drip tray, refer to the sample manager's overview and maintenance guide. For all other configurations, leak detection relies on a leak sensor installed in the drip tray of the lowermost column manager module. Page 77

78 To enable the column manager's leak sensor: 1. Power-on the column manager. 2. In the console, select System from the system tree. 3. Click Control > Leak Sensors, and select Column Manager, to enable the column compartment leak sensor Controlling the column compartment To control the CM-A and CM-Aux modules, enter the compartment temperature settings using the column manager control panels or method editor in the chromatography data software. For details, refer to the Waters online Help. Recommendation: When sample and column temperature are important to an application, in addition to specifying explicit temperature set points in the method, specify appropriate temperature limits. Together, these settings ensure that system operation occurs only within the defined limits and that any occurrence of an unacceptable deviation from the set points is flagged by an error message acknowledging the variance. 2.6 Responding to a leak-sensor alarm After approximately 1.5 ml of liquid accumulates in the leak-sensor reservoir, an alarm sounds, indicating that the leak sensor detected a leak. The location for leak detection varies according to the column manager configuration. In a singlestack configuration with the CM-A positioned atop the sample manager, the CM-A module's waste drips into the sample manager's upper drip tray. Accordingly, leak detection is afforded by the leak sensor in the sample manager's upper drip tray. For instructions on how to respond to the leak-sensor alarm for the sample manager, refer to the sample manager's overview and maintenance guide. For all other configurations, leak detection relies on a leak sensor installed in the drip tray of the lowermost column manager module. Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Page 78

79 Notice: To avoid scratching or otherwise damaging the leak sensor, do not allow buffered solvents to accumulate and dry on it; do not submerge it in a cleaning bath. Note: Small column leaks can be undetectable because the liquid leaked can evaporate before reaching the leak-sensor reservoir. Tools and materials Chemical-resistant, powder-free gloves Cotton swabs Nonabrasive, lint-free wipes To restore the column manager's leak sensor to service: 1. Power-off the column manager. 2. Open the column manager door. 3. Locate the source of the leak, and make the repairs necessary to stop it. Tip: Liquid from a detector leak can accumulate in the column manager leak sensor reservoir and cause the column manager leak sensor alarm to sound. Always inspect the detector when attempting to locate the source of a leak. 4. Remove the leak sensor from its reservoir, grasping it by its serrations, and pulling upward. Figure 2 42: Leak-sensor serrations Serrations 5. Use a nonabrasive, lint-free wipe to dry the leak-sensor prism. Page 79

80 Figure 2 43: Leak-sensor prism Leak-sensor prism 6. Roll up a nonabrasive, lint-free wipe, and use it to absorb the liquid from the leak-sensor reservoir and its surrounding area. 7. With a cotton swab, absorb any remaining liquid from the corners of the leak-sensor reservoir. 8. Align the leak-sensor T-bar with the slot in the side of the leak-sensor reservoir, and slide the leak sensor into place. Figure 2 44: Column manager leak sensor installed Leak sensor installed in leak-sensor reservoir 9. In the console, select Column Manager from the system tree. 10. In the column manager information window, click Control > Reset CM, to reset the column manager. Page 80

81 3 Maintenance Perform the procedures in this section during routine maintenance or when you discover a problem with a column-module component. For information about isolating problems in the column compartment, consult the troubleshooting section in the Waters console Help. 3.1 Contacting Waters Technical Service If you are located in the USA or Canada, report malfunctions or other problems to Waters Technical Service ( ). From elsewhere, phone the Waters corporate headquarters in Milford, Massachusetts (USA), or contact your local Waters subsidiary. The Waters Web site includes phone numbers and addresses for Waters locations worldwide. Visit When you contact Waters, be prepared to provide this information: Error message (if any) Nature of the symptom Serial number of the system module and its firmware version, if applicable Flow rate Operating pressure Solvent(s) Detector settings (sensitivity and wavelength) Type and serial number of column(s) Sample type and diluent Chromatography data software version and serial number System workstation model and operating system version Note: For an explanation about how to report shipping damages and submit claims, see the document Waters Licenses, Warranties, and Support Services. 3.2 Recommended maintenance schedule Perform the following routine maintenance on the module, to ensure reliable operation and accurate results. When using the system throughout the day (and on nights and weekends), or Page 81

82 when using aggressive solvents such as buffers, perform these maintenance tasks more frequently Recommended CM-A and CM-Aux routine maintenance schedule Maintenance procedure Replace the column Replace the ferrule on the column fitting Replace the APH assembly Replace the switching valve cartridge Replace the leak sensor Replace the column in-line filter unit frit Clean the instrument with a soft, lint-free cloth, or paper dampened with water Frequency During scheduled routine maintenance or as needed During scheduled routine maintenance or as needed As necessary As necessary As necessary During scheduled routine maintenance or as needed As necessary 3.3 Spare parts To ensure that your system operates as designed, use only Waters Quality Parts. Visit for information about Waters Quality Parts, including how to order them. 3.4 Safety and handling Observe these warning and caution advisories when you perform maintenance operations on your system. Warning: Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials. Consult the Material Safety Data Sheets regarding the solvents you use. Additionally, consult the safety representative for your organization regarding its protocols for handling such materials. Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Page 82

83 Warning: To avoid electric shock, do not remove protective panels from system modules. The components within are not user-serviceable. Notice: To avoid damaging the electronic components of the sample manager and the column heater or column heater/cooler, always power-off the sample manager and column heater/cooler before connecting or disconnecting the interconnect cable. 3.5 Configuring maintenance warnings Maintenance counters, if available for a particular component, provide information about real-time usage that can help you determine when to schedule routine maintenance for specific components. You can specify usage thresholds and maintenance warnings that alert you when a component reaches a specified threshold. Thus you can minimize unexpected failures and unscheduled downtime during important work. For information explaining how to specify maintenance warnings, consult the Waters console Help. 3.6 Replacing the column manager's leak sensor Perform this procedure if you must replace the leak sensor in the CM-A or the CM-Aux. If your system contains only a CM-A or a CM-A and one CM-Aux, the leak sensor is located on the CM-A. If your system contains a CM-A and two CM-Aux modules, the leak sensor is located on the lower CM-Aux. Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Required tool and materials: Chemical-resistant, powder-free gloves New leak sensor To replace the column manager's leak sensor: 1. Power-off the column manager. 2. Open the column manager door. 3. Press down on the tab, to detach the leak sensor connector from the front of the instrument. Page 83

84 Figure 3 1: Connecting the leak-sensor cable to the receptacle on front of column manager Leak-sensor connector receptacle Notice: To avoid damaging the leak sensor, do not grasp it by the ribbon cable. 4. Remove the leak sensor from its reservoir, grasping it by its serrations, and pulling upward on it. Leak-sensor serrations 5. Unpack the new leak sensor. 6. Align the leak sensor s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place. Figure 3 2: Aligning the leak-sensor T-bar Page 84

85 Leak-sensor T-bar Figure 3 3: Installing leak sensor in reservoir Leak sensor installed in leak-sensor reservoir 7. Insert the leak-sensor connector into the receptacle on the front of the instrument. 8. Power-on the column manager. 9. In the console, select Column Manager from the system tree. 10. In the column manager information window, click Control > Reset CM, to reset the column manager. 3.7 Replacing the ferrule on the column-inlet fitting Replace the column-inlet ferrule on the APH assembly fitting if the column is leaking. Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Required tools and materials Chemical-resistant, powder-free gloves Replacement ferrule Page 85

86 To replace the ferrule on the column fitting: 1. Unscrew the cap nut from the fitting. Figure 3 4: Unscrewing cap nut from fitting Cap nut Fitting 2. Slide the fitting off the tubing. Figure 3 5: Sliding fitting off tubing Tubing Fitting Note: If the assembly contains a captive ferrule, the ferrule remains locked in the fitting. 3. If the assembly contains a captive ferrule, remove the ferrule from the fitting. Figure 3 6: Removing ferrule from fitting Fitting Ferrule (captive ferrule shown) 4. Discard the used ferrule. Page 86

87 5. Install the new ferrule on the fitting. 6. Slide the fitting and ferrule onto the tubing. Figure 3 7: Sliding fitting and ferrule onto tubing Tubing Fitting Note: If it is a captive ferrule, the ferrule locks into the fitting. 7. Screw the cap nut onto the fitting. Figure 3 8: Screwing cap nut onto fitting Cap nut Fitting 3.8 Replacing the column manager switching-valve cartridge Before replacing the switching-valve cartridge, ensure that you have the correct part for your system type (ACQUITY regular or Bio). For a description of the correct parts for your system, see Identifying the plumbing components and connections. Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Page 87

88 Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Required tools and materials: Chemical-resistant, powder-free gloves New switching-valve cartridge 1/4-inch open-end wrench 2-mm hex wrench To replace the column manager switching-valve cartridge: 1. In the console, select Column Manager from the system tree. 2. In the column manager information window, click Column Selection. 3. In the Column Manager Set Column dialog box, select Waste. 4. Pull the valve forward, approximately 2 cm, and rotate the lock ring clockwise until it rests against the lock ring stop. Figure 3 9: Switching-valve cartridge Lock ring Lock ring stop Stator ID 2-mm hex screw Page 88

89 5. Use the 1/4-inch wrench to remove the fittings attached to the switching-valve cartridge. Tip: For instructions on removing fittings, see Removing the existing fittings and column components. 6. Rotate the lock ring counterclockwise, and use the 2-mm hex wrench to remove the hex screw at the 10 o clock position on the switching-valve cartridge. 7. Remove the switching-valve cartridge from the switching-valve assembly by pulling the cartridge straight forward. 8. Unpack the new switching-valve cartridge. 9. Ensure that the groove in the cartridge housing aligns with the groove on the drive clamp. Requirement: If the grooves fail to align, turn the drive clamp until they do align, taking care to avoid scratching the drive clamp or housing. Figure 3 10: Aligning the cartridge housing and drive clamp grooves Drive clamp Aligned grooves Switching-valve cartridge housing 10. Insert the new switching-valve cartridge into the switching-valve cartridge chamber. Requirement: Ensure that the new cartridge assumes the same orientation as the old cartridge. It must slide fully into the switching-valve assembly. If it does not, contact Waters Technical Service. 11. Insert the 2-mm hex screw at the 10 o clock position on the switching-valve cartridge, and tighten the screw using the 2-mm hex wrench. 12. Pull the valve forward, approximately 2 cm, and rotate the lock ring clockwise until it rests against the stopper. 13. Use the 1/4-inch wrench to reattach all fittings, and tighten them 1/4-turn beyond fingertight for existing fittings, or 3/4-turn beyond finger-tight for new fittings. 14. Rotate the lock ring counterclockwise to the 12 o clock position. Page 89

90 15. Push the switching-valve assembly in until it stops. 16. Ensure that the tubing is properly oriented so that the column compartment door is unobstructed when closed. 3.9 Removing a column During scheduled preventive maintenance or when changing methods, you can remove the column from the column compartment before replacing it with a new column of the same or different type. Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Required materials: 1/2-inch open-end wrench Chemical-resistant, powder-free gloves Replacement column To remove the column: 1. Stop any running mobile phase. 2. After allowing sufficient time for cooling, open the column manager door. 3. Push the latches atop the column compartment cover inward, to disengage them from the latch pins. 4. Tilt the column compartment cover forward, just enough to clear the valve drip tray. Page 90

91 Figure 3 11: Slightly opened column compartment (example showing CM-A) Valve drip tray Column compartment cover Latches Latch pins 5. Pull the column compartment cover up and away from the module. 6. Detach the column's ecord fob from the receptacle on the right-hand side of the column heater. 7. Remove the connected column components (column, column in-line filter, and APH assembly) in their entirety from the column compartment. 8. While holding the column, disconnect the column-outlet tubing. 9. Loosen the cap nut from the gold fitting on the APH assembly. Tip: If necessary, use the 1/2-inch open-end wrench to loosen the gold-plated, locking compression screw that attaches the APH assembly to the in-line filter or to the column. 10. Rotate the column (or column and in-line filter) off the gold fitting on the APH assembly. 11. To install the replacement column, continue with the instructions for connecting columns in Connecting the tubing. Page 91

92 3.10 Replacing the APH assembly Before replacing the APH assembly in the CM-A or CM-Aux, ensure that you obtained the correct replacement assembly for the system type, application (SST or MP35N), and length of the APHinlet tubing (32 cm or 47 cm) (12.5 in or 18.5 in) that you intend to use. When replacing the APH assembly, heed the following information: Warning: To avoid personal contamination with biohazards or compounds that are toxic, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment's internal temperature, to ensure all components are cool. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. Notice: To avoid damaging the pre-heater assembly, turn the column onto the pre-heater assembly when installing or removing the column to prevent the pre-heater tubing from twisting; do not grasp and turn the pre-heater assembly; do not suspend or hang the pre-heater tubing. Required tools and materials Chemical-resistant, powder-free gloves 1/4-inch open-end wrench 1/2-inch open-end wrench required when operating the system at 103,421 kpa (1034 bar, 15,000 psi) or higher Replacement APH assembly To replace the APH assembly: 1. Stop any running mobile phase. 2. After allowing sufficient time for cooling, open the column manager door. 3. Push the latches atop the column compartment cover inward, to disengage them from the latch pins. 4. Tilt the column compartment cover forward, just enough to clear the valve drip tray. Page 92

93 Figure 3 12: Slightly opened column compartment (example showing CM-A) Valve drip tray Column compartment cover Latches Latch pins 5. Pull the column compartment cover up and away from the module. 6. Using the 1/4-inch open-end wrench, disconnect the APH-inlet tubing from its assigned port on the CM-A module's inlet-switching valve. Tip: The port varies depending on which APH assembly you are replacing. For plumbing details, see Identifying the plumbing components and connections. 7. Gently pull the APH assembly from its retainer, and remove the assembled APH assembly, column (or column and in-line filter), and column-outlet tubing in their entirety from the compartment. Page 93

94 Figure 3 13: APH assembly and column components installed in CM-A or CM-Aux column trough APH assembly APH-inlet tubing APH gold fitting cap nut Column Column-outlet fitting Column-outlet tubing 8. While holding the column, disconnect the column-outlet tubing from the outlet of the column. 9. Loosen the cap nut from the gold fitting on the APH assembly. 10. Holding the gold fitting on the APH assembly, rotate the column (or the in-line filter and column) off the fitting. 11. Set aside the APH assembly for discarding. 12. Unpack the new APH assembly. 13. Using the 1/4-inch open-end wrench, connect the long, flat compression-screw end of the new APH assembly to the designated port on the CM-A module's inlet-switching valve. For detailed instructions about assembling the new fitting, see Assembling new fittings. 14. Route the APH-inlet tubing through the appropriate grooves in the column compartment to the inlet switching valve. 15. Loosen the cap nut from the gold fitting on the APH assembly. 16. Slide the gold fitting with ferrule into the inlet of the column, or the in-line filter. Note: For instructions explaining how to install the column in-line filter, see the document ACQUITY UPLC Column In-Line Filter Unit Installation Instructions. Page 94

95 17. Rotate the column or in-line filter onto the gold fitting of the APH assembly, and fingertighten it. 18. Tighten the cap nut onto the gold fitting of the APH assembly. Important: If you are operating the system at 103,421 kpa (1034 bar, 15,000 psi) or higher, finger-tighten the cap nut onto the compression screw, and then use the 1/2-inch open-end wrench to tighten the compression screw an additional 1/8 to 1/6-turn. 19. Reconnect the column outlet to the column-outlet tubing. 20. Carefully install the APH assembly into its retainer, aligning the column (or the in-line filter with column) inside the trough (see the figure above). 21. Press the column-outlet fitting into the clip, and rotate the clip so that the opening faces forward. 22. Carefully route the column-outlet tubing through the compartment door. 23. Start the mobile phase, examine the assembled components for leaks, and then tighten the fittings, if necessary Cleaning the exterior of the equipment Warning: To avoid electric shock, ensure that the electrical power to the equipment is interrupted; when cleaning the surface of the equipment, apply water to a cloth, and then wipe the instrument or device. Do not spray or otherwise apply water directly onto any equipment surface. Warning: To avoid personal injury, use eye and hand protection during the cleaning process. Requirement: Use eye protection when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. To clean the exterior of the equipment: Clean surfaces of the equipment using only a clean, soft, lint-free paper or clean cloth dampened with water. Page 95

96 A Safety advisories Waters instruments and devices display hazard symbols that alert you to the hidden dangers associated with a product s operation and maintenance. The symbols also appear in product manuals where they accompany statements describing the hazards and advising how to avoid them. This appendix presents the safety symbols and statements that apply to all of Waters product offerings. A.1 Warning symbols Warning symbols alert you to the risk of death, injury, or seriously adverse physiological reactions associated with the misuse of an instrument of device. Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices. The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device or component of an instrument or device. When one of these symbols appear in a manual s narrative sections or procedures, an accompanying statement identifies the applicable risk and explains how to avoid it. Warning: (General risk of danger. When this symbol appears on an instrument, consult the instrument s user documentation for important safety-related information before you use the instrument.) Warning: (Risk of burn injury from contacting hot surfaces.) Warning: (Risk of electric shock.) Warning: (Risk of fire.) Warning: (Risk of sharp-point puncture injury.) Warning: (Risk of hand crush injury.) Page 96

97 Warning: (Risk of injury caused by moving machinery.) Warning: (Risk of exposure to ultraviolet radiation.) Warning: (Risk of contacting corrosive substances.) Warning: (Risk of exposure to a toxic substance.) Warning: (Risk of personal exposure to laser radiation.) Warning: (Risk of exposure to biological agents that can pose a serious health threat.) Warning: (Risk of tipping.) Warning: (Risk of explosion.) A.1.1 Specific warnings A Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized: Wear eye protection. Extinguish all nearby flames. Do not use tubing that is, or has been, stressed or kinked. Do not expose nonmetallic tubing to compounds with which it is chemically incompatible: tetrahydrofuran, nitric acid, and sulfuric acid, for example. Be aware that some compounds, like methylene chloride and dimethyl sulfoxide, can cause nonmetallic tubing to swell, significantly reducing the pressure at which the tubing can rupture. Page 97

98 A Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans. Warning: To avoid infection with potentially infectious, human-sourced products, inactivated microorganisms, and other biological materials, assume that all biological fluids that you handle are infectious. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, Biosafety in Microbiological and Biomedical Laboratories (BMBL). Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the biohazard safety representative for your organization regarding the proper use and handling of infectious substances. A Biohazard and chemical hazard warning This warning applies to Waters instruments and devices that can process biohazards, corrosive materials, or toxic materials. Warning: To avoid personal contamination with biohazards, toxic materials, or corrosive materials, you must understand the hazards associated with their handling. Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Management of Chemical Hazards. Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the safety representative for your organization regarding its protocols for handling such materials. A.2 Notices Notice advisories appear where an instrument or device can be subject to use or misuse that can damage it or compromise a sample s integrity. The exclamation point symbol and its associated statement alert you to such risk. Notice: To avoid damaging the instrument s case, do not clean it with abrasives or solvents. A.3 Bottles Prohibited symbol The Bottles Prohibited symbol alerts you to the risk of equipment damage caused by solvent spills. Page 98

99 Prohibited: To avoid equipment damage caused by spilled solvent, do not place reservoir bottles directly atop an instrument or device or on its front ledge. Instead, place the bottles in the bottle tray, which serves as secondary containment in the event of spills. A.4 Required protection The Use Eye Protection and Wear Protective Gloves symbols alert you to the requirement for personal protective equipment. Select appropriate protective equipment according to your organization s standard operating procedures. Requirement: Use eye protection when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. A.5 Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Warning: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment. Avertissement : Toute modification sur cette unité n ayant pas été expressément approuvée par l autorité responsable de la conformité à la réglementation peut annuler le droit de l utilisateur à exploiter l équipement. Warnung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen. Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all'utilizzo dell'apparecchiatura da parte dell'utente. Advertencia: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo. Page 99

100 警告 未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作 該設備的權利 警告 未经有关法规认证部门明确允许对本设备进行的改变或改装 可能会使使用者丧 失操作该设备的合法性 경고: 규정 준수를 책임지는 당사자의 명백한 승인 없이 이 장치를 개조 또는 변경할 경우, 이 장치를 운용할 수 있는 사용자 권한의 효력을 상실할 수 있습니다. 警告: 規制機関から明確な承認を受けずに本装置の変更や改造を行うと 本装置のユーザーとして の承認が無効になる可能性があります Warning: Use caution when working with any polymer tubing under pressure: Always wear eye protection when near pressurized polymer tubing. Extinguish all nearby flames. Do not use tubing that has been severely stressed or kinked. Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids. Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic tubing to swell, which greatly reduces the rupture pressure of the tubing. Avertissement : Manipulez les tubes en polymère sous pression avec precaution: Portez systématiquement des lunettes de protection lorsque vous vous trouvez à proximité de tubes en polymère pressurisés. Eteignez toute flamme se trouvant à proximité de l instrument. Evitez d'utiliser des tubes sévèrement déformés ou endommagés. Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane (THF) ou de l'acide sulfurique ou nitrique concentré. Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture. Page 100

101 Warnung: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht: In der Nähe von unter Druck stehenden Polymerschläuchen stets Schutzbrille tragen. Alle offenen Flammen in der Nähe löschen. Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind. Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder konzentrierte Salpeter- oder Schwefelsäure verwenden. Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert. Avvertenza: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. Spegnere tutte le fiamme vive nell'ambiente circostante. Non utilizzare tubi eccessivamente logorati o piegati. Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico o nitrico concentrati. Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di rottura dei tubi stessi. Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión: El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos de polímero sometidos a presión. Si hubiera alguna llama las proximidades. No se debe trabajar con tubos que se hayan doblado o sometido a altas presiones. Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano (THF) o ácidos nítrico o sulfúrico concentrados. Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos. Page 101

102 警告 當在有壓力的情況下使用聚合物管線時 小心注意以下幾點 當接近有壓力的聚合物管線時一定要戴防護眼鏡 熄滅附近所有的火焰 不要使用已經被壓癟或嚴重彎曲管線 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸 要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹 大大降低管線的耐壓能 力 警告 当有压力的情况下使用管线时 小心注意以下几点 当接近有压力的聚合物管线时一定要戴防护眼镜 熄灭附近所有的火焰 不要使用已经被压瘪或严重弯曲的管线 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀 大大降低管线的耐压能 力 경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오. 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오. 근처의 화기를 모두 끄십시오. 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오. 비금속(Nonmetallic) 튜브를 테트라히드로푸란(Tetrahydrofuran: THF) 또는 농축 질 산 또는 황산과 함께 사용하지 마십시오. 염화 메틸렌(Methylene chloride) 및 디메틸술폭시드(Dimethyl sulfoxide)는 비금속 튜브를 부풀려 튜브의 파열 압력을 크게 감소시킬 수 있으므로 유의하십시오. 警告: 圧力のかかったポリマーチューブを扱うときは 注意してください 加圧されたポリマーチューブの付近では 必ず保護メガネを着用してください 近くにある火を消してください 著しく変形した または折れ曲がったチューブは使用しないでください 非金属チューブには テトラヒドロフラン(THF)や高濃度の硝酸または硫酸などを流さないでくださ い 塩化メチレンやジメチルスルホキシドは 非金属チューブの膨張を引き起こす場合があり その場 合 チューブは極めて低い圧力で破裂します This warning applies to Waters instruments fitted with nonmetallic tubing. This warning applies to instruments operated with flammable solvents. Page 102

103 Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Avertissement : L utilisateur doit être informé que si le matériel est utilisé d une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d être défectueuses. Warnung: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwendung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren. Avvertenza: si rende noto all'utente che l'eventuale utilizzo dell'apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall'apparecchiatura. Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes. 警告 使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用 那麼該設備所 提供的保護將被消弱 警告 使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用 那么该设备所 提供的保护将被削弱 경고: 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다. 警告: ユーザーは 製造元により指定されていない方法で機器を使用すると 機器が提供している 保証が無効になる可能性があることに注意して下さい A.6 Warnings that address the replacing of fuses The following warnings pertain to instruments and devices equipped with user-replaceable fuses. Information describing fuse types and ratings sometimes, but not always, appears on the instrument or device. Finding fuse types and ratings when that information appears on the instrument or device: Warning: To protect against fire, replace fuses with those of the type and rating printed on panels adjacent to instrument fuse covers. Page 103

104 Avertissement : pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués sur le panneau à proximité du couvercle de la boite à fusible de l'instrument. Warnung: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert auf den Tafeln neben den Sicherungsabdeckungen des Geräts gedruckt sind. Avvertenza: per garantire protezione contro gli incendi, sostituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate sui pannelli adiacenti alla copertura fusibili dello strumento. Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo y características impresos en los paneles adyacentes a las cubiertas de los fusibles del instrumento. 警告 為了避免火災 更換保險絲時 請使用與儀器保險絲蓋旁面板上所印刷之相同類 型與規格的保險絲 警告 为了避免火灾 应更换与仪器保险丝盖旁边面板上印刷的类型和规格相同的保险 丝 경고: 화재의 위험을 막으려면 기기 퓨즈 커버에 가까운 패널에 인쇄된 것과 동일한 타 입 및 정격의 제품으로 퓨즈를 교체하십시오. 警告: 火災予防のために ヒューズ交換では機器ヒューズカバー脇のパネルに記載されているタイプお よび定格のヒューズをご使用ください Finding fuse types and ratings when that information does not appear on the instrument or device: Warning: To protect against fire, replace fuses with those of the type and rating indicated in the Replacing fuses section of the Maintenance Procedures chapter. Avertissement : pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués dans la rubrique "Remplacement des fusibles" du chapitre traitant des procédures de maintenance. Warnung: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert im Abschnitt "Sicherungen ersetzen" des Kapitels "Wartungsverfahren" angegeben sind. Avvertenza: per garantire protezione contro gli incendi, sostituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate nel paragrafo "Sostituzione dei fusibili" del capitolo "Procedure di manutenzione". Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo y características indicados en la sección "Sustituir fusibles". Page 104

105 警告 為了避免火災 更換保險絲時 應使用 維護步驟 章節中 更換保險絲 所指 定之相同類型與規格的保險絲 警告 为了避免火灾 应更换 维护步骤 一章的 更换保险丝 一节中介绍的相同类 型和规格的保险丝 경고: 화재의 위험을 막으려면 유지관리 절차 단원의 퓨즈 교체 절에 설명된 것과 동 일한 타입 및 정격의 제품으로 퓨즈를 교체하십시오. 警告: 火災予防のために ヒューズ交換ではメンテナンス項目の ヒューズの交換 に記載されているタ イプおよび定格のヒューズをご使用ください A.7 Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument s front or rear panels. Symbol Description Electrical power on Electrical power off Standby Direct current Alternating current Alternating current (3 phase) Safety ground Frame, or chassis, terminal Fuse Functional ground Input Output Page 105

106 A.8 Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped. Symbol Description Keep upright! Keep dry! Fragile! Use no hooks! Upper limit of temperature Lower limit of temperature Temperature limitation Page 106

107 B Specifications The reproducibility of the specifications presented in this document depends on the conditions in individual laboratories. Contact the Waters Technical Service organization for additional information about specifications. B.1 CM-A and CM-Aux physical specifications Attribute Height Width Depth Weight Access to columnswitching valves Access to APH assembly, column inline filter, column, and column-outlet tubing Column heating and cooling Number of columns Column sizes Column selection Specification CM-A: 20.0 cm (7.8 in) CM-Aux: 13.7 cm (5.4 in) 34.3 cm (13.5 in) 61.0 cm (24.0 in) CM-A: 21.0 kg (46.0 lb) CM-Aux: 11.3 kg (25.0 lb) Inside CM-A module via access door Inside column compartment via removal of compartment cover Two column troughs (heating and cooling zones) per module with right and left APH control ports for installation of APH assembly, supporting active pre-heating Two, 150-mm columns with column guard or in-line filter Four, 50-mm columns with no column guard or in-line filter (CM-A only) Six columns, maximum, for a CM-A configuration with upper and lower CM-Aux modules 4.6-mm ID, maximum Column guards or in-line filters, 20-mm length, maximum Automated via two 9-port, 8-position valves; provides programmable, automatic, random access switching, waste and bypass positions for rapid solvent changeover Page 107

108 B.2 CM-A and CM-Aux environmental specifications Attribute Specification Acoustic noise <58 dba Ambient operating temperature 4 to 40 C Ambient operating humidity 20 to 80%, noncondensing Ambient shipping and storage temperature -30 to 60 C Ambient shipping and storage humidity 20 to 85%, noncondensing B.3 CM-A and CM-Aux electrical specifications Attribute Protection class a Overvoltage category b Specification Class I II Pollution degree c 2 Moisture protection d Line voltages, nominal Power requirements Line frequency Power consumption Normal (IPXO) Grounded AC 100 to 240 Vac 50 to 60 Hz 400 VA a. Protection Class I The insulating scheme used in the instrument to protect from electrical shock. Class I identifies a single level of insulation between live parts (wires) and exposed conductive parts (metal panels), in which the exposed conductive parts are connected to a grounding system. In turn, this grounding system is connected to the third pin (ground pin) on the electrical power cord plug. b. Overvoltage Category II Pertains to instruments that receive their electrical power from a local level such as an electrical wall outlet. c. Pollution Degree 2 A measure of pollution on electrical circuits that can produce a reduction of dielectric strength or surface resistivity. Degree 2 refers only to normally nonconductive pollution. Occasionally, however, expect a temporary conductivity caused by condensation. d. Moisture Protection Normal (IPXO) IPXO means that no Ingress Protection against any type of dripping or sprayed water exists. The X is a placeholder that identifies protection against dust, if applicable. Page 108

109 B.4 CM-A and CM-Aux input/output specifications Attribute Specification Upper and lower control connectors Control and communication between the CM-A and the CM-Aux Ethernet (CM-A only) Communication with chromatography data software B.5 CM-A and CM-Aux performance specifications Item Settable temperature range Controllable temperature range Specification 4.0 to 90.0 C, in 0.1 C increments (Ambient -25 C) to 90.0 C Temperature Tested to ±0.5 C accuracy a Test conditions: Door closed No column installed No flow Measurement taken with traceable, external temperature measurement device Measurement taken after 1 hour of thermal equilibration at set point Measurement taken at column compartment sensor location Tested at 35 C, 55 C, and 90 C Time to return to steady-state temperature on door open/close 12 minutes maximum Test conditions: No column installed No flow Measurement taken with internal temperature sensor Measurement taken after 1 hour of thermal equilibration at set point Door is opened for 30 seconds Tested at 35 C, 55 C, and 85 C Page 109

110 Item Temperature precision Specification Tested to ±0.1 C Test conditions: Door closed No column installed No flow Temperature stability Tested to ±0.3 C Test conditions: Measurement taken with traceable, external temperature measurement device Measurement taken at column compartment sensor location Temperature is ramped from ambient to 90 C Measurement taken after 1 hour of thermal equilibration Temperature is returned to ambient Test is repeated four additional cycles Door closed No column installed No flow Measurement taken with traceable, external temperature measurement device Measurement taken for 1 hour after thermal equilibration at set point Measurement taken at column compartment sensor location Tested at 35 C, 60 C, and 90 C Ambient temperature stability Pre-heater temperature (not user-settable) Solvent conditioning Leak control Column tracking Within 2.0 C/60 minute maximum Defined by set point of the column zone Active pre-heating Compartment drip tray with leak sensor installed under compartment. Single exit drain manages leaks to waste. ecord technology column information management tracks and archives column usage history a. Operating with active pre-heating via the APH assembly Page 110

111 B.6 CM-A and CM-Aux wetted materials of construction Component Material System tubing and fittings, APH assembly 316 SST, MP35N (Bio) Column-switching valve cartridge (CM-A only) 316 SST, Titanium (Bio) Column outlet tubing and fittings 316 SST, MP35N (Bio), PEEK Drip tray Polyethylene Drain tubing Tygon 2475 Page 111

112 C External valve installation and configuration The CM-A supports as many as three external valves. The information presented in this appendix provides specifications and basic instructions about how to install and configure the valves. Consult Waters Technical Service for additional information about operating the CM-A with external valves. The following table lists the supported external valves that you can connect to a CM-A. Table C 1: CM-A external valves External valve 10-port, 2-position, Titanium 7-port, 6-position, PEEK 11-port, 10-position, stainless steel C.1 Physical arrangement You can install the external valves above, or beside, the system stack. However, because they have no integrated leak management, you must place the valves in a drip tray that can channel any leakage to a suitable waste container, in accordance with your laboratory practices. C.2 Connecting an external valve You connect as many as three external valves to the column manager using a 3-gang, RS-232 serial-adapter cable. Each external valve must have a unique address (0, 1, or 2). Required tools and materials: Waters external valve (up to three) 3-gang, RS-232 serial-adaptor cable Female-to-female, serial cable coupler adapter (up to three, one per external valve) 1/8-inch flat-blade screwdriver Page 112

113 To connect one or more external valves to the column manager: 1. Depending on whether you are using one external valve or two or more valves, specify the valve addresses as follows: If you are using one external valve and its address is not already 0, use the flat-blade screwdriver to turn the screw on the back panel of the valve, setting the address to 0. If you are using more than one external valve, set the address of the second valve to 1 and that of a third valve to 2. Important: The turn-screw on the back of the external valve must align precisely with the address number. Otherwise, the external valve cannot operate. 2. Connect the standard end of the 3-gang, RS-232 serial-adapter cable labeled "RS-232 B" to the COM B RS-232 port on the column manager's rear panel. Figure C 1: CM-A rear panel showing COM B RS-232 port connection COM B RS-232 port Standard end of the 3-gang, RS-232 serial-adapter cable 3. Connect the external valve's serial cable to a female-to-female serial cable coupler adapter, and then connect the coupler adapter to the connector labeled "1" on the 3-gang RS-232 serial-adaptor cable. Figure C 2: Serial cable connections Page 113

114 External valve's serial cable connector Female-to-female serial cable coupler adapter Connector on 3-gang RS-232 serial-adaptor cable Note: If you are connecting more than one external valve, connect the second external valve's serial-adaptor cable to a female-to-female serial cable coupler adapter, and then connect the coupler adapter to the connector labeled "2" on the 3-gang RS-232 serialadaptor cable. Connect a third external valve's serial-adapter cable to a female-to-female serial cable coupler adapter, and then connect the coupler adapter to the connector labeled "3" on the 3-gang RS-232 serial-adaptor cable. 4. Connect the male end of each external valve's power cord to a suitable ac power outlet. C.3 Configuring an external valve To configure one or more external valves: 1. With the external valves connected to the ac power outlet, power-on the column manager. 2. In the console, from the system tree, select Column Manager. 3. For each external valve, cycle power to the external valve by disconnecting its ac power cord from the ac outlet, and then reconnecting it to the ac outlet. 4. In the column manager information window, click Control > Reset CM. 5. In the column manager information window, click Configure > Operating Mode. The Operating Mode Configuration dialog box appears. Page 114

115 Figure C 3: Operating Mode Configuration dialog box 6. In the Operating Mode Configuration dialog box, under "Select valve configuration", select the valves that you are configuring (External 1, External 2, or External 3). 7. For each external valve, specify the associated positions setting according to the valve's capability (2, 6, or 10). 8. Click OK, and then reboot the column manager when prompted. C.4 Controlling an external valve via method settings After you configure the external valves in the console, you can manually operate the valve by selecting the valve position from the console's column manager instrument page. To control the external valves via method settings, you must program the external valve position that you want for each external valve that is configured with the Column Manager mode selected for "Column Selection". Page 115

116 Figure C 4: Column Manager instrument method settings for external valve operation Page 116