Replacing the Column Stabilizer Tube with Finger-tight Fittings

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Carol Gibson
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1 , REV. H 1 OF 9 Replacing the Column Stabilizer Tube with Finger-tight Fittings This document explains how to replace the column stabilizer tube assembly with finger-tight fittings in the ACQUITY UltraPerformance LC System column heater and column heater/ cooler. a It includes instructions for: replacing the column stabilizer tube reconnecting the column stabilizer tube to a new column installing the column outlet fitting installing and disassembling the finger-tight fitting NOTE: Use this procedure when replacing a column stabilizer tube. If you are installing a new column stabilizer tube, column, or column in-line filter, refer to the ACQUITY UPLC System Operator s Guide (PN ). NOTE: For information on installing finger-tight reusable fittings on a column manager, see Waters procedure Installing Finger-tight Fittings in the Column Manager, available in the Waters Support Center ( Parts Required This procedure requires the column stabilizer kit for 50/100-mm columns or 150-mm columns (Table 1 and Figure 1). Use the part that corresponds to your column size. NOTE: For spare parts related to finger-tight fittings, see page 2. Table 1: Column Stabilizer Tube Assembly Part Number Description Quantity Column Stabilizer Kit, 50/100 mm Column Stabilizer Kit, 150 mm 1 Figure 1 - Column Stabilizer Kits (Use the Part Corresponding to Column Size) a. To be certain you have the latest version of this procedure, please visit the Support Center at Waters.com.

2 , REV. H 2 OF 9 Finger-tight Fitting Spare Parts (Order as Necessary) Tables 2, 3, and 4 list the spare parts available for finger-tight fittings (also see Figures 2 and 3); order them as needed. The collet and ferrule are the most commonly replaced parts. NOTE: Ferrules can be reused indefinitely as long as they function properly. Replace the ferrule only if it is damaged or fails to hold pressure. Table 2: Finger-tight Reusable Fitting Kit a Part Number Description Quantity Finger-tight Reusable Fitting Kit 1 Kit Components: 1/16 PEEK Ferrule (HPFT) 1 Collet (HPFT) 1 Gold Compression Screw (HPFT) 1 Collet Separator (original design, with no spiked end) 1 a. Use this kit when updating the column stabilizer tubing from the old-style inlet fitting to the finger-tight fitting. Table 3: Finger-tight Reusable Fitting Spare Parts a /16 PEEK Ferrule (HPFT) 10/pk Collet (HPFT) 2/pk Gold Compression Screw (without holes) 2/pk Collet and Compression Screw Multi-tool (Collet Separator) Column Support Clip (Column Heater) 10/pk Column Support Clip (Column Heater/ Cooler) 10/pk a. Order these parts when replacing any of the existing finger-tight fitting components. Table 4: Outlet Fitting Spare Parts Part Number Description Quantit y One-piece Black Fitting (High-temperature Outlet Fitting) Flangeless Right-angle Nut Extender (Outlet Fitting Wrench) 10/pk 1

3 , REV. H 3 OF 9 Tools/Materials Required Figure 2 - Finger-tight Fittings ACQUITY UPLC System Operator s Guide (PN ) 5 / 16 wrench Collet separator (included in kit) (Figure 3) Flangeless right-angle nut extender (outlet fitting wrench) (Figure 3) Procedure Figure 3 - Finger-tight Fitting Tools The column stabilizer tube is connected to a column or in-line filter on the column end, and to port 6 on the injector end. This procedure will outline the steps for: Replacing a column stabilizer tube Installing the outlet tube Reconnecting an old column stabilizer tube to a new column or in-line filter Replacing the Column Stabilizer Tube 1. Open the column compartment door 2. To gain access to the column heater tray:

715000926, REV. H 4 OF 9 a. (In a column heater): Lift up the metal latch and tilt the column heater tray downward (Figure a). Figure 4 - Accessing the Column Heater Tray b.

4 , REV. H 4 OF 9 a. (In a column heater): Lift up the metal latch and tilt the column heater tray downward (Figure a). Figure 4 - Accessing the Column Heater Tray b. (In a column heater/cooler): Swing the column retainer rods out to the sides (Figure 5). Figure 5 - Accessing the Column Stabilizer Tube (Column Heater/Cooler) Removing the Old Column Stabilizer Tube (if Necessary) 1. If there is an old-style column stabilizer tube installed, remove it: a. Disconnect the outlet fitting from the column outlet. b. Disconnect the inlet fitting to the column or in-line filter. c. Disconnect the old column stabilizer from injector port 6 (Figure 6).

6 1. Remove the clear laminate from the new thermal gasket adhesive back.

(Figure 7). NOTE: The gasket color may vary from that shown in the image.

5 , REV. H 5 OF 9 Installing the New Stabilizer Figure 6 - Disconnecting the Column Stabilizer from Port 6 1. Remove the clear laminate from the new thermal gasket adhesive back. Then, firmly apply the adhesive side of the gasket around the new stabilizer tubing block (Figure 7). NOTE: The gasket color may vary from that shown in the image. Figure 7 - Positioning the Thermal Gasket 2. Insert the column stabilizer tubing block into the opening on the right end of the column heater or heater/cooler tray (Figure 8). Figure 8 - Installing the Column Stabilizer Tubing Block

715000926, REV. H 6 OF 9 3. Remove the plastic retainer from the inlet end of the column stabilizer tube, and then connect the inlet tube to port 6 of the injector (Figure 9).

Remove the plastic retainer from the outlet end of the column stabilizer tube; then make sure that the ferrule is about 3 mm away from the outlet tube and that the collet is touching the back of the

6 , REV. H 6 OF 9 3. Remove the plastic retainer from the inlet end of the column stabilizer tube, and then connect the inlet tube to port 6 of the injector (Figure 9). Installing the Finger-tight Fitting Figure 9 - Connecting the Column Stabilizer Tube 1. Remove the plastic retainer from the outlet end of the column stabilizer tube; then make sure that the ferrule is about 3 mm away from the outlet tube and that the collet is touching the back of the ferrule (Figure 10). Figure 10 - Correct Positioning of Finger-tight Fittings 2. Push the outlet tubing into the column or in-line filter inlet until it stops. 3. Holding the finger-tight reusable fitting in place, rotate the column onto the fitting until it is snug. 4. Finger-tighten the fitting until it is snug. Then add a quarter-turn. CAUTION: TO PREVENT DAMAGE TO THE TUBING, USE ONLY YOUR FINGERS TO TIGHTEN THE FITTING.

715000926, REV. H 7 OF 9 Installing the Outlet Fitting 1. To reconnect the outlet tubing, insert the PEEK tubing into the high-temperature outlet fitting until it stops (Figure 11).

7 , REV. H 7 OF 9 Installing the Outlet Fitting 1. To reconnect the outlet tubing, insert the PEEK tubing into the high-temperature outlet fitting until it stops (Figure 11). CAUTION: TO PREVENT BANDSPREADING, ENSURE THAT THE TUBE IS FULLY BOTTOMED IN THE FITTING HOLE BEFORE TIGHTENING IT. Figure 11 - Installing the Column 2. Holding the PEEK tubing, thread the high-temperature outlet fitting to the right until it is finger-tight. 3. Using a 5 / 16 wrench to hold the column steady, use the high-temperature outlet fitting wrench to give the outlet fitting a quarter turn (Figure 12) CAUTION: TO AVOID DAMAGING THE OUTLET FITTING, DO NOT OVER-TIGHTEN IT WITH THE OUTLET FITTING WRENCH. Figure 12 - Tightening the High-temperature Fitting

8 , REV. H 8 OF 9 4. Place a second high-temperature column support clip between the finger-tight reusable fitting and column, rotating the clip so that the opening faces forward (Figure 13). Figure 13 - Installing the Second Column Support Clip 5. Dress the PEEK tubing back into the slot on the left-hand side of the column channel. 6. Secure the tube: a. (Column heater): Close the column heater tray and push down the metal latch. b. (Column heater/cooler): Swing the column retainer rod to the left to fasten it. Reconnecting a Column Stabilizer to a New Column NOTE: You must disassemble the finger-tight fitting before reconnecting it to a new column or in-line filter. Replacing the Column 1. Using the outlet fitting wrench, disconnect the high-temperature outlet fitting. Figure 14 - Connecting the Column Stabilizer to a New Column

9 , REV. H 9 OF 9 2. Pull the column stabilizer with attached column out of the column tray. 3. Grip the gold compression screw and rotate the column to detach it from the screw. CAUTION: TO AVOID DAMAGING THE TUBING, DO NOT ROTATE THE COMPRESSION SCREW OFF THE COLUMN. 4. Replace the column (see Waters ACQUITY UPLC System Operator s Guide). Disassembling the Finger-tight Fitting You must disassemble the finger-tight fitting before reconnecting it to a column. 1. Place the forked end of the collet separator over the collet on the column stabilizer tube, and gently pry the collet loose from the gold compression screw. Figure 15 - Disassembling the Finger-tight Fitting 2. Replace the ferrule only if it is damaged or fails to hold pressure. Ferrules can be reused indefinitely as long as they function properly. CAUTION: DO NOT REPLACE THE FERRULE WITH ANY OTHER TYPE OF FERRULE. USE ONLY THE 5 / 16 HPFT PEEK FERRULE ( ). 3. Follow the steps for installing the finger-tight fitting (page 6). 4. Follow the steps for installing the outlet fitting (page 7).

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