Dilutor Module for TriPlus RSH
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1 Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual Additional section of the TriPlus RSH User Guide and Hardware Manual Revision A December 2015
2 2015 Thermo Fisher Scientific Inc. All rights reserved. TriPlus RSH, TRACE 1300, TRACE 1310, TRACE GC Ultra and FOCUS GC are trademarks of Thermo Fisher Scientific Inc., and its subsidiaries. Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana Rodano-Milan, Italy Tel: ; Fax: Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc. The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser. This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents. Release history: Revision A, December 2015 Original Instructions For Research Use Only. Not for use in diagnostic procedures.
3 C Contents Chapter 1 Dilutor Module for TriPlus RSH Safety Alerts, Important Information and Warnings Module Description Naming Conventions Dilutor Module Specifications Hardware and Software Requirements Unpacking the Components Getting Started Installing the Dilutor Valve Installing the Dilutor Syringe Installing the Dilutor Syringe Module Installing the Dilutor Tool Connecting the Transfer Tubing Electrical Connections Setting Up the Dilutor Syringe Module Dilutor Module Parameters Options Menu Items for Dilutor (x) Setting Up the Dilutor Tool Dilutor Tool Parameters Options Menu Items for DIL (x) Calibrating the Dilutor Tool Routine Operation and Limitations Maintenance and Troubleshooting Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual iii
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5 This manual is an additional section of the TriPlus RSH User Guide (P/N ) and TriPlus RSH Hardware Manual (PN ). It contains information for installing, operating, and maintaining the Dilutor module. Contents Safety Alerts, Important Information and Warnings Module Description Naming Conventions Dilutor Module Specifications Hardware and Software Requirements Unpacking the Components Getting Started Installing the Dilutor Valve Installing the Dilutor Syringe Installing the Dilutor Syringe Module Installing the Dilutor Tool Electrical Connections Setting Up the Dilutor Syringe Module on page 15 Setting Up the Dilutor Tool on page 20 Calibrating the Dilutor Tool on page 23 Routine Operation and Limitations on page 25 Maintenance and Troubleshooting on page 26 Note Items highlighted in green are only visible in the access level Extended User. Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 1
6 Safety Alerts, Important Information and Warnings Safety Alerts, Important Information and Warnings About the safety alerts, the important information and the series of warning refer to the chapter Preface of the TriPlus RSH User Guide and TriPlus RSH Hardware Manual. Module Description The Dilutor Module is an optional device for the TriPlus RSH System. Its main purpose is typically solvent handling, such as adding a solvent for dilution, a reagent for derivatization, spiking with an internal standard, and so on. Another widely used application is sampling an exact volume of an aliquot and pipetting it into another vessel for further processing. See Figure 1. Figure 1. Dilutor Module Two versions are available: Single Dilutor With a 3-port valve for 1/4-in.-28 fittings Multi Dilutor With a 7-port (6+) ceramic valve for 1/4-in.-28 fittings Naming Conventions Naming conventions for the Dilutor Module and its parts are listed below: Dilutor Module Describes the entire assembly of the Module. Dilutor Syringe Module Active module carrying the Dilutor Syringe and the Dilutor Valve. Dilutor Tool Describes the tool itself as part of the Dilutor Module. Dilutor Valve It is placed on the Dilutor Syringe Module and one of its ports is connected to the Dilutor Syringe. Two types are available: 2 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
7 Dilutor Module Specifications 3-Port (3P) PTFE Valve 7-Port (6+) Ceramic Valve Mounting Bracket Fixes the Dilutor Module to the X-Axis of the TriPlus RSH. Contains two holders for the Guiding Wire. Solvent Reservoir Kit Consists of one reservoir bottle (1 L) with tubing (PFA) and one glass frit with a pore size of 40 μm. Transfer Tubing Kit Connects the Dilutor Module and Dilutor Tool. Consists of the PFA Tubing, the Guiding Wire and connection fittings. Guiding Wire The Wire integrated into the Transfer Tubing. Dilutor Module Specifications The specifications of the Dilutor Module are: Dilutor Module Single Dilutor or Multi Dilutor Dilutor Valve 3-port (3P) valve for ¼-in.-28 fittings for Single Dilutor; 7-port (6+) Ceramic (Alumina) valve for ¼-in.-28 fittings for Multi Dilutor Maximum Permissible Pressure 3 bar (300 kpa; 43.5 psig) Dilutor Syringe 1 ml, Scale 60 mm; Optional 0.1 ml, 5 ml, 10 ml Transfer Tubing PFA tubing ID mm (1/32 ) / OD 1.58 mm (1/16 ) Including Connection Fittings and Guiding Wire Length of transfer tubing: 1530 mm Needle Gauge 22, Pst 3, Length 51 mm; Optional Gauge 23, Pst AS, Length 51 mm Solvent Reservoir Kit (1 piece for Single Dilutor; 5 pieces for Multi Dilutor) 1000 ml borosilicate glass including: Glass Filter, 40 μm pore size (1000-SV) Aspiration Tubing (PFA) Wetted Parts Tubing from Reservoir Bottle to Dilutor Syringe Module. Tubing: PFA Frit: Glass Filter Dilutor Syringe Module: Syringe: Borosilicate Glass Valve 3-port: PTFE / Kel-F Valve 7-port: Ceramic (Alumina) Transfer tubing from Pump to LCP Tool: Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 3
8 Hardware and Software Requirements Tubing: PFA Electrical Connectors Two BUS connectors for daisy chaining; M12, 4 position, male, reserved for future use. Firmware Version or higher The Dilutor Module dimensions, weights, operating and environmental requirements are detailed in Table 1 and Table 2. Table 1. Dilutor - Dimensions and Mass Length Dimension in mm (inches) Width 115 (4.52); 175 (6.89) including cable connectors Depth 260 (10.24) Height 430 (16.93) 550 (21.65) including transfer tubing Mass Dimension in kg (lbs) Dilutor Syringe Module 1.31 (2.89) Mounting Bracket (0.49) Associated Cable 0.15 (0.33) Solvent Reservoir Kit Aspiration Kit: 0.06 (0.13) Reservoir Bottle 1000 ml: 0.58 (1.28) Total Weight of Single Dilutor 2.32 (5.11) Total Weight of Multi Dilutor 4.88 (10.76) Table 2. Dilutor - Operating and Environmental Requirements Length Dimension in mm (inches) Operating Temperature Range 4 to 40 C Maximum Relative Humidity 80%, non-condensing Bench Space Consider bench space for the Wash Solvent reservoir bottles, size 1000 ml (or as required by customer) Altitude Limitations 3000 meters above sea level Hardware and Software Requirements Observe the compatibility between the Device Firmware and the RSH Firmware. The Dilutor Module used for the TriPlus RSH System is an active module which requires its own specific Device Firmware. 4 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
9 Unpacking the Components The Device Firmware is part of the archive file with the extension *.pack. The TriPlus RSH System already has the necessary software packages pre-loaded when shipped from the factory. The Dilutor Module requires the RSH Firmware or higher. Unpacking the Components The Dilutor Module is shipped in a dedicated box separated from the TriPlus RSH system. The box also includes the Solvent Reservoir Kit(s), each consisting of a 1000 ml glass bottle, a glass frit, and the corresponding Tubing Kit. The components of Single Dilutor and Multi Dilutor contained into the relevant shipping box are respectively detailed in Table 3 and Table 4. Table 3. Single Dilutor Components Component Quantity Dilutor Syringe Module 1 Syringe for Dilutor Syringe Module 1 ml, Scale 60 mm (pre-mounted) 1 Dilutor Valve 3-Port (1/4-28-in. Fitting) 1 Pre-mounted Mounting Bracket 1 Dilutor Tool 1 Solvent Reservoir Kit containing: 1 1 x Glass Bottle 1000 ml 1 x Aspiration Tubing Kit, consisting of: Tubing (PFA) Glass Frit 40 μm pore size Nut with feed-through holes Transfer Tubing Kit for Dilutor (1/16-in.) 1 Needle Gauge 22 Pst 3 (for syringes 5 μl 100 μl) (Set of 3) 3 (1 pre-mounted) Bus Daisy Chain Cable, length 850 mm 1 Table 4. Multi Dilutor Components (Sheet 1 of 2) Component Dilutor Syringe Module 1 Syringe for Dilutor Syringe Module 1 ml, Scale 60 mm 1 (pre-mounted) Dilutor Valve 7-Port ceramic (1/4-28-in. -Fitting) 1 Quantity Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 5
10 Getting Started Table 4. Multi Dilutor Components (Sheet 2 of 2) Component Quantity Mounting bracket 1 Dilutor Tool 1 Solvent Reservoir Kit containing: 5 1 x Glass Bottle 1000 ml 1 x Aspiration Tubing Kit, consisting of: Tubing (PFA) Glass Frit 40 μm pore size Nut with feed-through holes Transfer Tubing Kit for Dilutor (1/16-in.) 1 Needle Gauge 22 Pst 3 for syringes 5 μl 100 μl 3 (1 pre-mounted) BUS Daisy Chain Cable, length 850 mm 1 Getting Started For properly installing the Dilutor Module see the following sections: Installing the Dilutor Valve on page 6 Installing the Dilutor Syringe on page 8 Installing the Dilutor Syringe Module on page 9 Installing the Dilutor Tool on page 11 Installing the Dilutor Valve The valve is pre-mounted to the Dilutor Syringe Module. If for any reason it is necessary to change the valve, see the following procedures: To install the 3-port valve on page 6 To install the 7-port valve on page 7 To install the 3-port valve The Single Dilutor is equipped with a 3-port valve for enabling the handling of one solvent/reagent. A port is reserved for the syringe and another one for the tool. The 3-port valve is fixed to the valve drive of the Dilutor using the two provided screws. The valve coupling of the valve drive has one flat side, ensuring the correct installation of the valve. 6 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
11 Installing the Dilutor Valve 1. Attach the Dilutor Valve to the valve coupling, while slightly turning it to the left or to the right until it slides completely on the valve coupling. See Figure Turn the valve with the Dilutor Valve coupling until the single port points downwards and the holes in the housing match with those in the valve. See Figure Fix the valve with the two provided screws. See Figure 2. Figure 2. 3-Port Valve Installation Valve Drive Flat Side To install the 7-port valve The Multi Dilutor is equipped with a 7-port valve for enabling the handling of up to five solvents/reagents. A port is reserved for the syringe and another one for the tool. In most cases it is recommended to specify one of the five free ports as the waste port for flushing the valve. The 7-port valve is fixed to the valve drive of the Dilutor using the two screws integrated into the valve. These screws can be accessed from the front of the valve through the small holes using the provided Allen wrench. CAUTION Do not loosen the two screws on the front of the valve, otherwise the valve may leak. The valve coupling of the valve drive has one flat side, ensuring the correct installation of the valve. 1. Attach the valve to the valve coupling, while slightly turning it to the left or to the right, until it slides completely on the valve coupling. See Figure Turn the valve with the valve coupling until the single port points downwards and the holes in the housing match with those in the valve. See Figure Fix the valve with the two provided screws. See Figure 3. Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 7
12 Installing the Dilutor Syringe Figure 3. 7-Port Valve Installation Valve Drive Flat Side Installing the Dilutor Syringe This section provides the instructions for installing the syringe to the Dilutor Syringe Module. Note The syringe is pre-mounted to the Dilutor Syringe Module. To install the dilutor syringe 1. Screw the syringe clockwise from below into the lower port of the valve. See 1 of Figure Unscrew the screw from the valve drive and pull the plunger of the screw out until the hole in the plunger matches the hole in the valve drive. 3. Fix the plunger with the screw to the valve drive. See 2 of Figure 4. Figure 4. Dilutor Syringe Installation 8 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
13 Installing the Dilutor Syringe Module Installing the Dilutor Syringe Module The Dilutor Syringe Module is attached to the X-Axis using the provided mounting bracket. Only one mounting bracket can be installed onto a TriPlus RSH system. The mounting bracket can either hold up to two Dilutor Syringe Modules. See 1 of Figure 5. ATTENTION If the Safety Guard is already mounted to the TriPlus RSH system, you must remove it before installing the mounting bracket for the Dilutor Module. The Safety Guard bracket on the left side of the TriPlus RSH system will be replaced by the mounting bracket, so this also must be removed beforehand. For installing only one Dilutor Module use the front mounting position, close to the X-Axis. See 2 of Figure 5. Figure 5. Example of Dilutor Mounting Positions To install the dilutor syringe module on the TriPlus RSH system 1. Install the mounting bracket for the Dilutor on the left side of the X-Axis. The bracket is installed underneath the side cover of the X-Axis using the two holes at the back side. See 1 of Figure 6. The two front holes are reserved for the mounting bracket of the Handheld controller (terminal). 2. Screw the outer (higher) holder for the Guiding Wire into the bracket. See 2 of Figure 6. Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 9
14 Installing the Dilutor Syringe Module Figure 6. Mounting Bracket Installation 3. Attach the Safety Guard to the mounting bracket and fix it with the two screws provided. The Safety Guard Bracket is used on the right side of the X-Axis. See Figure 7. Figure 7. Installation of the Safety Guard to the Mounting Bracket Safety Guard Bracket 10 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
15 Installing the Dilutor Tool 4. Install the Dilutor Syringe Module to the front position of the mounting bracket, closer to the X-Axis, and fix it using the two screws provided. See Figure 8. Figure 8. Installation of the Dilutor Syringe Module to the Mounting Bracket Installing the Dilutor Tool Note For installing the Dilutor Tool, the ATC Station must be placed on the outermost left position of the X-Axis. The left leg of the system must be placed on the right side of the ATC Station. It might be useful to connect the ATC Station at the end of a daisy chain in doing so only one Bus connector is used and the ATC Station can be placed further left due to the free right connector. The front or back mounting position of the Dilutor Syringe Module on the mounting bracket corresponds to the position of the Dilutor Tool in the ATC Station. If only one Dilutor is added to the TriPlus RSH system, the Dilutor Syringe Module must be installed on the front position, of the mounting bracket, close to the X-Axis. The corresponding Dilutor Tool is set to Slot 1 of the ATC Station. The Guiding Wire is fixed to the lower holder on the mounting bracket. Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 11
16 Installing the Dilutor Tool If a second Dilutor is added, the second Dilutor Syringe Module must be installed on the back position of the mounting bracket. The corresponding tool must be set in Slot 2 of the ATC Station. The Guiding Wire is fixed to the higher holder on the mounting bracket. Figure 9 shows the installation of two Dilutor Modules. See also Connecting the Transfer Tubing on page 12. Figure 9. Installation of Two Dilutor Modules Connecting the Transfer Tubing To properly connecting the Transfer Tubing, see the following procedure: To connect the transfer tubing on page Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
17 Installing the Dilutor Tool To connect the transfer tubing 1. Connect the Transfer Tubing to the Dilutor Syringe Module. a. Place the end of the Guiding Wire in the lower holder of the mounting bracket. See 1 of Transfer Tubing Connection to the Dilutor Syringe ModuleFigure 10. b. Connect the Transfer Tubing to the Dilutor Valve. See 2 of Figure 10. c. Connect the Aspiration Tubing from the reservoir bottle to the Dilutor Valve. See 3 of Figure 10. Figure 10. Transfer Tubing Connection to the Dilutor Syringe Module Note The Guiding Wire of a second Dilutor Module must be placed in the higher holder of the mounting bracket. Note Never place the reservoir bottle(s) on a higher level than the TriPlus RSH system itself to avoid draining of the liquid in case of any leakage. WARNING The reservoir bottle(s) must be placed at the side of to the GC system away from any heated zones. 2. Connect the Transfer Tubing to the Dilutor Tool. Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 13
18 Installing the Dilutor Tool a. Insert the free end of the Guiding Wire into the corresponding holder on the Dilutor Tool and fix it with the integrated screw. See 1 and 2 of Figure 11. Figure 11. Transfer Tubing Connection to the Dilutor Tool b. Connect the free end of the Transfer Tubing to the needle in the Dilutor Tool. See 1 of Figure 12. Figure 12. Transfer Tubing Connection to the Needle of the Dilutor Tool c. Clamp the tubing in the groove on the back of the tool. 14 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
19 Electrical Connections Electrical Connections This section provides instructions for making the electrical connections of the Dilutor Module. WARNING Before starting the electrical connections make sure that the TriPlus RSH system is powered Off. Connect the supplied Dilutor Module control cable from one of the daisy chain connectors on the back side of the module to the TriPlus RSH Control Interface connector marked BUS. If the BUS connector is already occupied, connect the cable to another Active module with an open connector. For details see Figure 15. Figure 13. Dilutor Module Electrical Connections BUS Connector Daisy Chain Connectors Reserved Connector Not Used Setting Up the Dilutor Syringe Module Note There are two different ways to access the steps for setting up the Dilutor Syringe Module. 1. Using the Options menu from the Start Screen. See First Path on page Selecting the menu item Dilutor x from the Start Screen. See Second Path on page 17. Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 15
20 Setting Up the Dilutor Syringe Module First Path Use the Options Pull-up menu under the Start Screen. Select the access level Extended User then navigate to the Start Screen. See Figure 14. Figure 14. Dilutor Module Start Screen and List Items (1) The last two figures reflect the List Items of a Dilutor, while the Dilutor Valve is not yet specified. In the case of a 3-port Valve two Fluid Ports are configurable in the case of a 7-port Valve six Fluid Ports, are configurable. 16 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
21 Setting Up the Dilutor Syringe Module Second Path The Dilutor Syringe Module List Items are accessible directly from the Start Screen. See Figure 15. Figure 15. Dilutor Module Start Screen and List Items (2) Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 17
22 Setting Up the Dilutor Syringe Module Dilutor Module Parameters Table 5 lists Dilutor Module parameters. ] Table 5. Dilutor Module Parameters Item Description Dil. Bubble Detector Dil. Plunger Drive Dil. Valve Drive Fluid Port 1-8 Syringe Type Valve Type Dilutor Valve Current Pos. Port Home Pos. Port Number of Ports Plunger Homing Pos. Tool Pos. Port Offset Port 1-8 Switch Time Updates the parameters of the Bubble Detector. The actual position of the Plunger in μm. Read-only parameter. Shows the Actual Position in degree and the Valve Type of the Dilutor Valve. Read-only parameter. Specifies a name for each liquid at the corresponding port. The number of Fluid Ports corresponds to the number of ports of the Dilutor Valve installed. Selects the type of the Syringe in the Dilutor Syringe Module. Selects the Type of the Dilutor Valve in the Dilutor Syringe Module. Shows some read-only parameters of the Dilutor Valve. The currently selected port. The port defined as Home Position. The number of ports. The port used for homing the plunger. The port the Dilutor Tool is connected to. The offset in degrees for each port. Define the time for switching between the ports. Options Menu Items for Dilutor (x) Selecting the pull-up menu Options opens up the following tasks. See Table 6 and Figure 16. ] Table 6. Dilutor Module Pull-up menu Options (Sheet 1 of 2) Item Change Syringe Setup Module Description The plunger of the Dilutor is moved down to enable the exchange of the syringe. Sets up the configuration parameters for the Dilutor module and the Dilutor tool. 18 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
23 Setting Up the Dilutor Syringe Module Table 6. Dilutor Module Pull-up menu Options (Sheet 2 of 2) Item Prime Dilutor Home Plunger Home Drive Enable Drive Disable Drive Description A selectable Solvent Port of the Dilutor is primed. Specifies the Solvent Port as well as the total liquid volume for priming. The number of cycles is calculated by the total volume divided by the volume of the Transfer Tubing. The Plunger is moved to the home position and the Z-Axis is referenced. Moves the Dil. Plunger Motor to Home position. Enables the selected motor after it has been disabled. Disables the selected motor to permit moving it manually. Figure 16. Dilutor Module Options Menu (1) Selecting the item Setup Module from the Options Pull-up Menu allows you to setup the Dilutor Module and the corresponding Tool in one menu. Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 19
24 Setting Up the Dilutor Tool All parameters can also be adapted separately within the corresponding sub-menus of the Dilutor Tool and the Dilutor Module. Using the Options Pull-up menu in the sub-menus Dil. Plunger Drive and Dil. Valve Drive, the corresponding motor (drive) can be disabled or enabled. This functionality is useful if the plunger or the valve motor must be moved manually. See Figure 17. Figure 17. Menu Options (2) Setting Up the Dilutor Tool Note There are two different ways to access the steps for setting up the Dilutor Syringe Module. One path uses the Options menu from the Start Screen. See First Path on page 20. The second path is by selecting the menu item DIL x from the Start Screen. See Second Path on page 22. First Path The Options Pull-up menu under the Start Screen is used. Select the access level Extended User then navigate to the Start Screen. See Figure Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
25 Setting Up the Dilutor Tool Figure 18. Dilutor Tool Start Screen and List Items (1) Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 21
26 Setting Up the Dilutor Tool Second Path The Dilutor Tool List Items can be accessed directly from the Start Screen. See Figure 19. Figure 19. Dilutor Tool Start Screen and List Items (2) Dilutor Tool Parameters Table 7 lists the Dilutor Tool parameters. ] Table 7. Dilutor Tool Parameters Item Location Dilutor Description The location of the tool. Read-only parameter. Selects the corresponding Dilutor Module to which the tool will be connected. Instrument Length Length Ndl Guide Type Needle Type Trsf. Tube Vol. The total length of the instrument without the height of the instrument base plate. Read-only parameter. The total length of the instrument including the height of the instrument base plate. Read-only parameter. Selects the Type of the Needle Guide. Selects the needle type to use. Specifies the volume of the Transfer Tubing. The Volume is calculated using the following formula: V = π r 2 l where: V = volume of the tubing. r = half of the inner diameter of the tubing l = length of the tubing Typical values for the volume of the transfer tubing are in the range of 800 μl. 22 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
27 Calibrating the Dilutor Tool Options Menu Items for DIL (x) Selecting the pull-up menu Options opens up following tasks. See Table 8. ] Table 8. Dilutor Tool Pull-up menu Options Item Description Calibrate Tool Check Tool Calibration Select Tool Park Tool Calibrates the tool installed in the head. Checks the calibration point of the Tool installed in the head. Selects another tool. The currently attached tool is first parked and the newly-required tool is picked up. Parks a Tool without picking up another Tool from the ATC Station. Calibrating the Dilutor Tool Note Refer also to the section Calibrate Tools in the TriPlus RSH User Guide. The calibration of the motors is basically a check to determine whether the mechanical stop points of the various axes can be detected reliably. A clearance distance (offset) from the mechanical stop point is assigned and stored as the electronic stop point. This calibration of the axes is mandatory and ensures reliable performance. This step must be repeated if a motor has failed, has been replaced, or when any other major error has occurred. This calibration task brings the robot back to reliable operation. To calibrate the dilutor tool 1. Select the access level Extended User, then from the Start Screen select the item Dilutor Tool DIL1. See Figure 20. Figure 20. Dilutor Tool Calibration (1) 2. Press Options, select Calibrate Tool and follow the wizard. See Figure 21. Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 23
28 Calibrating the Dilutor Tool Figure 21. Dilutor Tool Calibration (2) Alternatively the Dilutor Tool can also be calibrated from the start screen using the path Options Service Installation Calibrate Tools. The wizard will guide you through the calibration process. Note It is recommended to create a backup file when a module has been added or moved within the TriPlus RSH system. 24 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
29 Routine Operation and Limitations Routine Operation and Limitations Routine Operation Always filter freshly prepared washing solvents. Recommended mesh size is 0.47 mm or finer. If pure water is used, be aware of possible biological growth. To avoid biological growth, one can add an organic modifier. The maximum concentration allowed depends upon the application, but it should not exceed 15 vol% of methanol, 10 vol% acetonitrile, or 5 vol% 2-propanol. After refilling or changing the solvent(s) in the reservoir bottle, always use the priming functionality to ensure that all lines are completely bubble-free and that the solvent has been properly exchanged. If biological samples are analyzed, ensure that the first wash step is performed with aqueous solvent. The second wash step can be done with an organic solvent, but the final wash step must be with an aqueous solvent again. Not observing this procedure may lead to protein precipitation and as a consequence, clogging of the lines. The final wash step is important to prepare the system for the next sampling, and to ensure that the needle is free from protein precipitate. WARNING When filling-up standard reservoirs or replacing a solvent, such as a washing solvent, remove the solvent reservoir bottle from the system to avoid a possible spill over the instrument. Depending on the physical, chemical, and hazardous properties of the solvent, use the appropriate protective measures for handling. Limitations Currently, the following limitations are known: The Dilutor Valve is designed to bear a back-pressure of approximately 3 bar (300 kpa; 43.5 psig). In case of a higher back-pressure leakage of the valve may occur. The 7-Port Ceramic Dilutor Valve should not be operated with highly pure water otherwise a degradation of the Ceramic Structure may result. The Size of the Dilutor Syringe is limited to the following assortment of offered Syringe Sizes: 0.1 ml, 1 ml, 5 ml, and 10 ml. Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 25
30 Maintenance and Troubleshooting Maintenance and Troubleshooting Routine Maintenance Regular maintenance procedures help to ensure the accuracy and precision of the TriPlus RSH system. Suggested intervals of maintenance procedures are included to ensure uninterrupted operations. If you use the system extensively, for example, nights and weekends, or if you use corrosive solvents, you must perform the maintenance procedures more frequently. See Table 9. WARNING Before starting a maintenance make sure that the TriPlus RSH is powered Off. Table 9. Maintenance Steps Maintenance Steps Clean the outside of the instrument. Use only a soft, lint-free cloth dampened with mild soap and water. Check the integrity of the lines and connections. Check the flow of the solvent and check for any blockage. Check the cleanliness of the solvent frit in the solvent reservoir bottle. Check the integrity of the Dilutor Syringe. Interval Weekly or as needed. Daily or as needed. Daily or as needed. Daily or as needed. Daily or as needed. Troubleshooting This section gives a quick overview of possible causes and recommended actions which can be taken to eliminate an erratic behavior. See Table 10. Table 10. Troubleshooting (Sheet 1 of 2) Symptom Syringe Needle bending. Solvent flow not sufficient. Air bubbles in solvent lines. Recommended Actions Check the teaching position for X-, Y-, and Z-Axes. Check the cleanliness of the solvent frits. Check the solvent levels in the reservoir bottles. Check the cleanliness of the solvent frits. Check the flow by using the task PrimeWashLiner. 26 Dilutor Module for TriPlus RSH Instruction Manual Thermo Scientific
31 Maintenance and Troubleshooting Table 10. Troubleshooting (Sheet 2 of 2) Symptom Solvent flow blocked. Recommended Actions Check the solvent level in the reservoir bottles. Check the cleanliness of the solvent frits. Check the integrity of the lines and connections. Dilutor Module not available for TriPlus RSH system. Low Precision in dispensing solvent. Guiding Wire with Transfer Tubing catches against other parts of the system. Dilutor Syringe worn out within short time. Activate the priming function (path described above) and check systematically for the blockage. Check the electrical connections from the module to the Bus connector (or daisy chaining to the next module). Check the back-pressure of the Transfer Line to the Needle. The Dilutor Valve is tight to approximately 3-4 bar ( kpa; psig). Check for air bubbles reduce the speed of Aspirating/ Dispensing in case of air bubbles. Check the position of the Dilutor Syringe Module. Check if the Guiding Wire is twisted. Check for a correct installation of the Dilutor Tool. See Installing the Dilutor Tool on page 11. Check for particles in the Dilutor Syringe and in the Liquid Filter. Thermo Scientific Dilutor Module for TriPlus RSH Instruction Manual 27
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