Array I: Schematic Diagram of TranSignal Protein/DNA Array I
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- Clementine Marsh
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1 TranSignal Protein/ rrays User anual Page P-1 P-1 P-2 P-2 P-1 P-1 R R rn-3 rn-3 P P c-yb c-yb P-1 P-1 P-2 P-2 P-1 R P-1 R rn-3 rn-3 P P c-yb c-yb R R R 2-1 R ts ts ts-1/pea-3 ts-1/pea R R ST-1 ST-1 S/SR S/SR P-2 P-2 R R R R R R ts ts ts1/pea3 ts1/pea3 ST-1 ST-1 S/SR S/SR P-2 P-2 T T R/PR R/PR -4-4 R-1 R yc/ax yc/ax -1 T -1 T R/PR R/PR R-1 R-1-2 yc/ax yc/ax T-1 T k k -1/YY1 T T Pax-5 Pax-5-1/YY1 Pbx-1 Pbx-1 T-1 T-1-1/YY1-1/YY k k T T Pax-5 Pax-5 Pbx-1 Pbx-1 PPR PPR PR PR RR/R-5 RR/R-5 RXR/R-1 RXR/R-1 S S Smad/S Smad/S S-3/4 S-3/4 PPR PPR PR PR RR/R-5 RR/R-5 RXR/R-1 RXR/R-1 S S Smad/S Smad/S S-3/4 S-3/4 Sp-1 Sp-1 Sp-1 SR TR Sp-1 TR SR TR/R-4 TR TR TR/R-4 SR SR TR/R-4 TR/R-4 rray : Schematic iagram of TranSignal Protein/ rray US-1 US-1 US-1 US-1 VR/R-3 VR/R-3 VR/R-3 VR/R-3 rray V: rey rows and columns are spotted with biotinylated and are used as postive controls for the assay. Pink shaded Rows are the same T s as the T s above in the white rows except that they are spotted at a 1:10 dilution. or some Transcription actors, mutliple consensus sequences for the same T have been publsihed in the literature and where appropriate, we have provided those variations on our arrays. or the T s with mutliple consensus sequences, they have been denoted with numbers in parenthesis, ie,, etc. To obtain the consensus sequences and references for the T s, used in our arrays, please call our Technical Support group at or at techsupport@panomics.com. S S S S R R R R T T T T
2 Page 16 TranSignal Protein/ rrays User anual R-1-1 R-2 V-1 fi-1-1 SR T-ox R PPR Tax/R R-1-1 R-2 V-1 fi-1-1 SR T-ox R PPR Tax/R rray : Schematic iagram of TranSignal Protein/ rray R/RT -2 R-3 R S S -3 SR US-1 p300 PPR T/ R/RT -2 R-3 R S S -3 SR US-1 p300 PPR T/ -R TP/R R-4 R T-1 S -3β - Z-1 PRP RR X-1 -R TP/R R-4 R T-1 S -3β - Z-1 PRP RR X-1 ntioxident R UP-T R-5 fxh/ oxo4 T-1/2 S/S -4 -Y PX-2 RR XR ntioxident R UP-T R-5 fxh/ oxo4 T-1/2 S/S -4 -Y PX-2 RR XR P-3 R-1 47 reac-2 T / UP-T 2 kx-2.5 PX-3 RSR4 Z P-3 R-1 47 reac-2 T / UP-T 2 kx-2.5 PX-3 RSR4 Z P-4 c-r 4-4 reac-2 T-3-2 karos 2 PS-2 PX-4 S Z P-4 c-r 44 reac-2 T-3-2 karos 2 PS-2 PX-4 S Z β-response element R-0 4/T reac-4 T-4-3 R-1/2-3 S/ X-1 PX-6 Skn Z β-response element rray : rey rows and columns are spotted with biotinylated and are used as postive controls for the assay. or some Transcription actors, mutliple consensus sequences for the same T have been publsihed in the literature and where appropriate, we have provided those variations on our arrays. or the T s with mutliple consensus sequences, they have been denoted with numbers in parenthesis, ie,, etc. To obtain the consensus sequences and references for the T s, used in our arrays, please call our Technical Support group at or at techsupport@panomics.com. R-0 4/T reac-4 T-4-3 R-1/2-3 S/ X-1 PX-6 Skn Z dx/ X2 R-1 lk-1 reak-7 T-6-8 SR SP-1 Z-3 PX-8 SRY Z dx/ X2 R-1 lk-1 reak-7 T-6-8 SR SP-1 Z-3 PX-8 SRY Z
3 TranSignal Protein/ rrays User anual Page 17-1 T/R sl-1 y-1 Z-1 p55 R SYR WT rray : Schematic iagram of TranSignal Protein/ rray -1 T/R sl-1 y-1 Z-1 p55 R SYR WT -1/ RP-1 T P1/T/ T -1 P-1 Z -4 P3/P RX-1/2/3 T WT 1/ RP-1 T P1/T/ T -1 P-1 Z -4 P3/P RX-1/2/3 T WT P-1 P X4 TP-1 T-Zf -ma P RP-3a1 T-3 WT P-1 P X4 TP-1 T-Zf -ma P RP-3a1 T-3 WT / T R-2 4T X 8/9/10 R-1 T -ma P-2 S-1 Tf- X2 / T R-2 4T X 8/9/10 R-1 T -ma P-2 S-1 Tf- X2-1 S -1 X8-1 yo --2 S-2 TT-3 X-1/ X2-1 S -1 X8-1 yo --2 S-2 TT-3 X-1/ X2 P-2/YY1 28R T -8/ 3 U X8 R-1 yo -Y S-3 T-1 Z174 P-2/YY1 28R T -8/ 3 U X8 R-1 yo -Y S-3 T-1 Z174 R-6 28R 12 i X- 9/10 y yt R-1 Pur-1 SP-1/SP TR-1/2 rray : rey rows and columns are spotted with biotinylated and are used as postive controls for the assay. or some Transcription actors, mutliple consensus sequences for the same T have been publsihed in the literature and where appropriate, we have provided those variations on our arrays. or the T s with mutliple consensus sequences, they have been denoted with numbers in parenthesis, ie,, etc. To obtain the consensus sequences and references for the T s, used in our arrays, please call our Technical Support group at or at techsupport@panomics.com. R-6 28R 12 i X- 9/10 y yt R-1 Pur-1 SP-1/SP TR-1/2 RP dx-2-40/45-40/45 S y Z-1 P53 PYR SR/SP v-af RP dx-2 S y Z-1 P53 PYR SR/SP v-af
4 Page 18 TranSignal Protein/ rrays User anual P-2 ZP c-ts-1 R 44 T 4T R- 2/im1 rray V: Schematic iagram of TranSignal Protein/ rray V P-2 ZP c-ts-1 R 44 T 4T R- 2/im1-1 P-3 c-fos enhancer YP11-80 TV4/ β/ 11α -4α 2/1 S S -1 P-3 c-fos enhancer YP11-80 TV4/ β/ 11α -4α 2/1 S S P-4 P c-myb -1-2/ -2/ mu3/y3 mu3/y3 ra-1/u ra-1/u -1 X4 k- XR-1 P-4 P c-myb -1-1 X4 k- XR-1 P apo 1 PR (4) c-yc dioxin receptor gastrin R i- S distal lactoferrin S-1 P apo 1 PR (4) c-yc dioxin receptor gastrin R i- S distal lactoferrin S-1-2/3/4 Tadelta (5) UP-T 12/47 R-1 T-1 i-/ 1T1 S proximal /3/ α-p Tadelta actor 1 T-α T-α β-globin (5) UP-T P-1 P-1 P-1 12/ T-1-1/2/3-1/2/3 Y5 Y5 R-1 R-2 R-2 2-1/2/3 i-/ -1 i-3 i-3 1T1 a/b/c S proximal -1-1 S/T -2-1 S/T α-p β-globin actor 1 P-1 2-1/2/3-1 a/b/c rray V: rey rows and columns are spotted with biotinylated and are used as postive controls for the assay. or some Transcription actors, mutliple consensus sequences for the same T have been publsihed in the literature and where appropriate, we have provided those variations on our arrays. or the T s with mutliple consensus sequences, they have been denoted with numbers in parenthesis, ie,, etc. To obtain the consensus sequences and references for the T s, used in our arrays, please call our Technical Support group at or at techsupport@panomics.com. -2
5 TranSignal Protein/ rrays User anual Page 19 mck PR 1 yb - k PX-2 PT-1 SP-1b b tp X-1 rray V: Schematic iagram of TranSignal Protein/ rray V mck PR 1 yb - k PX-2 PT-1 SP-1b b tp X-1 myc/1 T-1 -ras PX-5 PU.1 Snail Surf-2 transferrin XR myc/1 T-1 -ras PX-5 PU.1 Snail Surf-2 transferrin XR myc/pr -tp T PX-6 P- PUR SPR-1 T3R TT-1 Y-1 myc/pr -tp T PX-6 P- PUR SPR-1 T3R TT-1 Y-1-2a -tx T P PR-c RR/RXR 1/67 SR Tat TT-1-2a -tx T P PR-c RR/RXR 1/67 SR Tat TT-1 fh P PR-1 TxR fh P PR-1 RX-1/2/3 RX-1/2/3 SR SR T-3 T-3 TxR gene PR W ox -1-6/ P-1 P-2 PR RR SSP T UR gene PR W ox -1-6/ P-1 P-2 PR RR SSP T UR msx-1/2/3 msx-1/2/ rray V: rey rows and columns are spotted with biotinylated and are used as postive controls for the assay. or some Transcription actors, mutliple consensus sequences for the same T have been publsihed in the literature and where appropriate, we have provided those variations on our arrays. or the T s with mutliple consensus sequences, they have been denoted with numbers in parenthesis, ie,, etc. To obtain the consensus sequences and references for the T s, used in our arrays, please call our Technical Support group at or at techsupport@panomics.com. PR= Promoter, = inding Protein. -3 k PX1 PP PR R PT-1β RV /3 Thy-1 WP -3 k PX1 PP PR R PT-1β RV /3 Thy-1 WP
6 Page 20 TranSignal Protein/ rrays User anual P P-1 P-2 R m-3 P c-yb R 2-1 R R ts ts/p3 ast-1 S/ SR T R/PR R yc-ax -1 T-1-1/ YY1-2 k T PX-5 Pbx1 PPR PR RR/ R-5 RXR/ R-1 S S/ S S- 3/4 Sp-1 SR omborray: Schematic iagram of Protein/ omborray Stat 6 T TR TR/ R-4 US-1 VR/ R-3 S R R-1 hr/mt 4/T -R ntioxident R P-3 P-4 eta-r dx/ X UP-T c-rel lk-1 V-1 T-6 - PX-3 R R fxh/ oxo4 reac-2 reac-2 reac-4 TP-R reac-7 S T-1 T-2 R-/2 p300 T-3 T-4-1 PX / UP-T karos SR SR -3 T-ox US-1 Z-1 -Y kx-2.5 PS-2 Z-3 R PRP PX-2 PX-6-2 SP-1 PX-8 PPR PPR RR S Skn SRY Tax/ R T/ X-1 XR -1-1 P-1 / -1 R-6 RP RSR4 T/R T T TP-1-4 RP3a1-1 R-1 28R dx-2 P1/T /T P X4 Z P R-2 S T 12 28R -8/3 /U i -1 X-8 X-8 S sl-1 P S-2 R-1 y y y T-Zf T yo yo yt Z1 Z1 -ma -Y R-1 p55 P-2 PYR R RX-1/ 2/3 S-1 S-3 SP-1/ SP T SR SP T3 Tf- TT-3 T-1 Pur-1 TR-1/2 v-af WT WT WT X2 X-1 X2 Z174-1 P -1 P-2 P-3 T adelta T-a β glob factor 1 ZP (4) (5) c-ts-1 c-os c-myb c-yc P-1 P-1 R R-1 R-2 T ra-1/ U UP-T T-1-1/2 /3/Y5 4T /47 i- i-/ T-1 i/3-1 a/b/c -4a 2/1-6- R- S k- R- -1 T- 1/2-40/45 X- 8/9/10 X- 9/10 alpha- P - 1/2/3 - YP11 11/11a actoferrin / im-1 S XR X-4-2a omborray: rey rows and columns are spotted with biotinylated and are used as postive controls for the assay. or some Transcription actors, mutliple consensus sequences for the same T have been publsihed in the literature and whe appropriate, we have provided those variations on our arrays. or the T s with mutliple consensus sequences, they have been denoted with numbers in parenthesis, ie,, etc. To obtain the consensus sequences and references for the T s, used in our arrays, please call our Technical Support group at or at techsupport@panomics.com. R= Respnose lement = inding Protein fh-1 W box msx- 1/2/3 yb myc/1 myc/pr -1-1/ -3 T-1 -tp -tx -2-6/ P1 k k -ras T PX-1 PX-2 PX-5 PX-6 P P PP PR P- PR-c PR- 1 PR PT-1β PT-1 PU.1 PUR RX-1/ 2/3 RR RV Snail SPR-1 SR SSP /3 b Surf-2 T3R Tat T-1/ P-5 T1 T-3 T Thy-1 Transferrin TT-1 TxR - UR v-rel 50-55k WP X-1 Y-1 P
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