QuickSplit TM Adjustable Flow Splitters Analytical Splitters Analytical range, 0.1 ml/min. to 5 ml/min. input flow, calibration flow rate 1.0 ml/min.

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1 Adjustable Flow ters Applications Post-column splitting is fairly straight forward. Like pre-column flow splitting, any significant additional pressure (resistance) down stream from the splitter may affect the split ratio. Post-column devices also contribute chromagraphic dispersion so care must given connecting tubing and fittings, especially at low flow rates. Use the back pressure vs split ratio chart on page 17 and 18 select a Quick Adjustable Flow ter that will provide the desired split ratio range and back pressure. These charts correspond several flow rates and solvent systems. The back pressure is directly proportional flow rate and viscosity. The back pressure estimates on these charts only apply post-column applications. ters are shipped complete with the resisr cartridge installed. To assure optimum pressure drop across the splitter, please specify the actual inlet flow rate when the splitter is ordered if it is significantly different from the calibration flow rate. Cusm Ratios ratios and resisr cartridges other than those listed below can be ordered from ASI cusm configure the Quick Adjustable Flow ter. Please contact technical support for additional information about cusm splitters. We will gladly assist you in determining the best splitter configuration for your application. Quick TM Adjustable Flow ters ters range, 0.1 ml/min. 5 ml/min. input flow, calibration flow rate 1.0 ml/min PO PO PO PO-06 Cusm 600-PO-CS 1 Replacement Resisr Cartridges Distribur: Adjustable Flow ter Resisr Cartridge, Adjustable Flow ter Resisr Cartridge, Adjustable Flow ter Resisr Cartridge, Adjustable Flow ter Resisr Cartridge, Adjustable Flow ter Resisr Cartridge, Cusm CS ERC GmbH Ot-Hahn-Strasse D Riemerling

2 Adjustable Flow ters Applications continued ters Semi-Prep range, 5 ml/min. 40 ml/min. input flow, calibration flow rate 20.0 ml/min. 1 20,000: PO ,000: PO : PO PO20-03 Cusm 600-PO20-CS Replacement Resisr Cartridges Adjustable Flow ter Resisr Cartridge, Adjustable Flow ter Resisr Cartridge, Adjustable Flow ter Resisr Cartridge, Adjustable Flow ter Resisr Cartridge, Adjustable Flow ter Resisr Cartridge, 1 20,000: ,000: : Cusm CS 2 Preparative ters Prep range, 40 ml/min. 1,000 ml/min. input flow, cusm calibration flow rate Preparative Adjustable Flow ter,, Input Flow Range: ml/min. Preparative Adjustable Flow ter,, Input Flow Range: ml/min. Preparative Adjustable Flow ter,, Input Flow Range: - 1,000 ml/min. Preparative Replacement Resisr Cartridges Preparative Adjustable Flow ter Resisr Cartridge,, Input Flow Range: ml/min. Preparative Adjustable Flow ter Resisr Cartridge,, Input Flow Range: ml/min. Preparative Adjustable Flow ter Resisr Cartridge,, Input Flow Range: - 1,000 ml/min. Distribur: Cusm Cusm Cusm Cusm Cusm Cusm ERC GmbH Ot-Hahn-Strasse D Riemerling 600-PO30-CS 600-PO40-CS 600-PO60-CS CS CS CS ERC GmbH Ot-Hahn-Strasse D Riemerling

3 Adjustable Flow ters Quick Adjustable Flow ter Selection Charts 00 Back pressure vs ratio for water at 1 ml/min. 600-PO range 600-PO PO PO PO-04 Figure ratio 3 00 Back pressure vs ratio for water at 20 ml/min. 600-PO20 Semi-prep range 600-PO PO PO PO20-02 Distribur: Figure ratio ERC GmbH Ot-Hahn-Strasse D Riemerling ERC GmbH Ot-Hahn-Strasse D Riemerling

4 Adjustable Flow ters Quick Adjustable Flow ter Selection Charts Back pressure vs ratio for 50/50 ACN/water at 1 ml/min. 600-PO range PO PO PO PO-04 1 Figure ratio 4 Back pressure vs ratio for 50/50 ACN/water at 0.5 ml/min. 600-PO range PO PO PO PO ratio Figure 15 Distribur: ERC GmbH Ot-Hahn-Strasse D Riemerling ERC GmbH Ot-Hahn-Strasse D Riemerling

5 Adjustable Flow ters Pre-Column Applications Pre-column splitting is used for micro, capillary, and nano HPLC applications, where the flow from the pump is split from analytical flow rates down microliter or nanoliter flows. It is important note that even though the split ratio created by the splitter valve will remain constant, the split ratio will change when a HPLC column is installed. This is due the added resistance on the low flow rate channel from the HPLC column. This added resistance must be facred in make sure the fluid resisr selected for the flow splitter provides the correct split ratio. Please contact ASI if you need assistance. The charts on page 17 and 18 only estimate the pressure drop across the splitter for post column applications and do not include the HPLC column back pressure. ters are shipped complete with the resisr cartridge installed. When ordering a pre-column flow splitter, please provide ASI with the column flow rate and back pressure. If the inlet flow rate or column pressure specification is not provided, ASI will configure pre-column flow splitters assuming a 0.5 ml/min. inlet flow rate and a pressure drop across the column of 1,500 PSI. Cusm Ratios ratios and resisr cartridges other than those listed below can be ordered from ASI cusm configure the Quick Adjustable Flow ter. Please contact technical support for additional information about cusm splitters. We will gladly assist you in determining the best splitter configuration for your application. Quick TM Adjustable Flow ters ters range, 0.25 ml/min. 1 ml/min. input flow, calibration flow rate 0.5 ml/min. These splitters will produce under 3,500 PSI backpressure with water at 0.5 ml/min. Adjustable Flow ter, Pre-Column Adjustable Flow ter, Pre-Column Adjustable Flow ter, Pre-Column Adjustable Flow ter, Pre-Column Adjustable Flow ter, Pre-Column PR PR PR PR-06 Cusm 600-PR-CS 5 Replacement Resisr Cartridges Adjustable Flow ter Resisr Cartridge, Pre-Column Adjustable Flow ter Resisr Cartridge, Pre-Column Adjustable Flow ter Resisr Cartridge, Pre-Column Adjustable Flow ter Resisr Cartridge, Pre-Column Adjustable Flow ter Resisr Cartridge, Pre-Column PR PR PR PR00-06 Cusm 600-PR00-CS Distribur: ERC GmbH Ot-Hahn-Strasse D Riemerling

6 Adjustable Flow ters Adjustable Flow ter Accessories Replacement Inlet Filters Inlet Filter, 2 micron.063" dia. 5/Pack, 1 µl Volume Inlet Filter, micron.125" dia. 5/Pack, 4 µl Volume Straight Thru Hole, No Filter 5/Pack, 1 µl Volume Flow ter Adjustable FS - Semi-Prep Range & Prep Range for 600-PO30, Adjustable FS - Post/Pre-column Range Adjustable FS - Prep Range for 600-PO Capillary Resisr Capillary Resisr Cusm 600-PR00-CP Flow Measurement Kits Flow Rate Range 50 nl/min. Flow Rate Range 5 µl/min. 5 µl/min. 25 µl/min. tubing 360 µm OD fused silica 1/16 OD PEEK tubing S S 6 Flow Rate Range µl/min. µl/min. 1/16 OD PEEK tubing 600-0S Flow Rate Range 25 µl/min. 500 µl/min. 1/16 OD PEEK tubing S Distribur: ERC GmbH Ot-Hahn-Strasse D Riemerling

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