LC Instrument Replacement Parts

Transcription

1 LC Instrument Replacement Parts /SSI Parts /SSI Pump Replacement Parts Alltech Pump Replacement Parts Misc. Pump Supplies Agilent Parts & Supplies for Agilent Instrumentation 1260 Infinity Isocratic LC Supplies Purge Valves Inlet & Outlet Vales Piston Seals Seal Wash Solvent Reservoirs Solvent Filters Miscellaneous Capillaries Vacuum Degassers Injection Valves Needles & Needle Seats Metering Device Supplies Autosampler Trays Fraction Collector Supplies Manual Injection Valves Thermostatted Column Compartment Supplies Detector Supplies Variable Wavelength Detector (VWD) Supplies Diode Array Detector (DAD)/Multiple Wavelength Detector (MWD) Supplies Refractive Index Detector (RID) Supplies Fluorescence Detector (FLD) Supplies LC/MS Supplies & Chemicals LC/MS Foreline Pump Supplies LC/MS Common Supplies LC/MS Calibrant Mixes Laboratory Solvent Bottles Instrument Replacement Parts by Instrument Vendor Introduction to ASI, Analytical Scientific Instruments Introduction to Optimize Technologies For Agilent/HP Instruments For Beckman/Altex Instruments For PerkinElmer Instruments For Shimadzu Instruments For Thermo Scientific Instruments For Waters Instruments

2 Featured Products 331 Alltech Pump Replacement Parts Agilent Solvent Filters Agilent Long Life Lamps Have an Alltech Pump? has original manufacturered replacement parts Contact Support Desk for help choosing parts CHROM TECH RELATED PRODUCTS A-242 A A B JT-PP12755 JT-TF12752-CC1 6 Port Manifold Vapor Filter Solvent Waste Container Kit, page 262 Upchurch Scientific General Use Inlet Stainless Steel Solvent Filters, page 268 ASI Static Mixers, page 245 Female Ports are Coned accept 1/16" OD tubing Standard Assemblies are rated to 5,000 PSI. UHPLC Assemblies are rated to 15,000 PSI Notes from the Support Desk (continued on next page) LC Instrument Replacement Parts

3 Notes from the Support Desk HPLC Pump Trouble Shooting Guide The following trouble shooting guide has been prepared by ASI for the novice as well as experienced service technicians. Often it is more expensive to simply replace old parts with new ones and get back to wrok, but, for the chromatographer who doesn t have the budget for that, or who is down on a weekend with no spare parts, we hope these tips get you HPLC pump up and running, and keep it running. PROBLEM CAUSE REMEDY Erratic Pressure Dirty inlet valve Remove inlet valve and flush with 50 ml of clean HPLC grade solvent. Dirty outlet valve Clogged solvent intake filter Leak at solvent inlet line Replace with new valve (an inlet cartridge can be substituted for an outlet if no outlet valves are available). If there is no filter on the outlet, then it may be cleaned in the same manner as an inlet. Replace with new filter. Tighten fitting. Replace ferrule and fitting if no longer serviceable. Note: This problem may not be easy to detect! On the intake side of the pump there is a slight vacuum, so air will leak into the pump rather than solvent leaking out. There will be no visible sign of a leak. To detect this problem make sure that there is no air in the intake lines, then open the outlet fitting and watch for air bubbles coming out (place a drop of solvent at the outlet fitting to observe air bubbles). If you continue to observe air bubbles after 60 seconds, then air is probably entering the head from the inlet fitting, a leaky pump seal, or an insufficiently tightened inlet housing. Wom pump seal or piston Replace seal. Inspect piston and replace if worn. Note: If your piston is worn, you can sometimes get a PTFE seal to work for a short time, until a new piston can be obtained. PTFE will generally conform to and seal with a worn piston better than UHMW-PE, but not for long. Also, PTFE will generate copious amounts of wear material when used with a worn piston. Replace the worn piston ASAP. Air in pump head Purge pump for 30 seconds, then operate at 1,000 PSI or higher. The air will dissolve in the solvent and be expelled within about 30 minutes. Note: This symptom is normal after replacing a pump seal or piston. Wet the seal and inside of the pump head with IPA to reduce the amount of time it takes to eliminate air from the head. Frequent Check Valve Failure Not using degassed solvents Air bubbles in inlet line Contaminated solvent No solvent inlet filter Degas, preferably using vacuum and sonication Degas solvent. Replace solvent inlet filter. Make sure solvent botttle still has solvent! Use clean HPLC grade solvent. Always use 10 micron or finer solvent filter. Notes from the Support Desk Wom pump seal or Piston Replace seal. Inspect piston and replace if worn. Note: If the seal is badly worn, then the pump head will be contaminated with seal wear material. Remove the check valves and seal from head and sonicate the head in 20% nitric acid for 30 minutes. Rinse thoroughly, then sonicate for 10 minutes in DI water. Wet seal and pump head with IPA prior to reassembly. The inlet valve should be flushed with 50 ml of HPLC grade IPA or Water, and the outlet valve filter inspected (if there is no filter on the outlet, then flush with 50 ml HPLC solvent). Service life of check valves has been exceeded Install new valves. Failure to Prime Excess back pressure in pump head Open purge valve, or open fittings at outlet check valve. Valve installed upside down Clogged solvent inlet filter Clogged outlet valve filter Verify that arrow faces up for both inlet and outlet valve. Clean or replace with new filter. Replace outlet valve filter. Note: The outlet valve filter should last at least 2 years in normal service. However, if piston seals are wearing out frequently, then wear material from the seal may cause premature clogging of the outlet frit, as well as contaminating the inlet valve. Replace the outlet filter, and inspect the sapphire piston for wear or score marks. 328 your chromatography specialists

4 Notes from the Support Desk HPLC Pump Trouble Shooting Guide (cont.) PROBLEM CAUSE REMEDY Operating Pressure is Lower than Normal Seal Life Unusually Short Worn seal or piston Air in pump head or intake line Clogged solvent inlet filter Dirty inlet valve Dirty outlet valve Leaky fitting Replace seal. Inspect piston and replace if worn. Purge pump for 30 seconds, then operate at 1,000 PSI or higher. The air will dissolve in the solvent and be expelled within about 30 minutes. Clean or replace with new filter. Remove inlet valve and flush with 50 ml of clean HPLC grade solvent. Replace with new valve (an inlet cartridge can be substituted for an outlet if no outlet valves are available). If there is no filter on the outlet, then it may be cleaned in the same manner as an inlet. Tighten fitting. Replace ferrule if badly deformed. Note: If a slight amount of additional tightening does not slow stop the leak, then replace the ferrule. Over-tightening the fitting can damage the seat, strip the threads, or worse, cause the nut to shear off. Never exceed 15 in-lbs of torque. Worn or scratched piston Replace with new piston. Note: It is not always obvious by visual inspection if a piston is worn or scratched. Use a magnifying glass to look for a glazed appearance, or axial grooves and scratches. The piston is badly worn if any such conditions are visible. Build-up of salts on piston Mobile phase incompatible with seal material Use the piston flush option if available. Always run DI water through the pump before shutting down for the day when running buffers. Use a PTFE seal. ASI Replacement Piston Seals Selecting the Best Material for your Application UHMWPE Compound This material is based on ultra-high molecular weight polyethylene, and is compounded with other proprietary polymers to provide better lubricity and solvent resistance than pure UHMWPE. This material is a self-lubricating compound that exhibits excellent wear characteristics, low coefficient of friction, and good resistance to cold-flow (extrusion). It is often used for dry bearings and seals, where lubrication by oil or other fluids is not permitted. It is resistant to most HPLC solvents except for methylene chloride and toluene. UHMWPE seals should only be used intermittently when using these solvents in 100% concentration. ASI offers two different formulations of this material. One is a gold color and the other is white. The gold material is slightly stiffer than the white material, while the white material exhibits a slightly lower coefficient of friction. The formulation that we feel is most suitable for a specific pump model is the only formulation of UHMWPE that we offer for that pump model. There are many different UHMWPE formulations that appear the same color, but they are not all the same. PTFE Compound This material is PTFE with a filler added to it for the purpose of increasing resistance to cold flow and improving wear characteristics. It is absolutely inert to any hplc solvent, but does not have the high resistance to wear and cold-flow that the UHMWPE compounds have. It is more flexible that UHMWPE, and therefore will conform better with pistons that are not aligned well, or which are worn flat. The color of our formulation is black. There are dozens of PTFE compounds, many of which are black, but they are not the all same. Some offer an extremely low coefficient of friction but wear rapidly, while others last longer but can rapidly wear the sapphire piston (such as ceramic filled PTFE). The selection of the PTFE compound that we use is based on thousands of hours of testing of compounds from several different suppliers, and we are confident that it offers the best performance. Conclusion In general we recommend using our UHMWPE compound. It generates less wear material than PTFE, tends to act as a better bearing to help align the piston, and has a much longer service life than PTFE. Always inspect the piston for signs of scoring or wear when replacing seals. If your piston is worn or scratched, replace it, because nothing will destroy a seal faster than a worn or scratched piston. Notes from the Support Desk

5 LC INSTRUMENT REPLACEMENT PARTS /SSI Parts /SSI Pump Replacement Parts MAX PUMP FLOW RATES 5-6 ml/min ml/min 10 ml/min ml/min 40 ml/min FOR LEGACY PUMPS: P-1500, Q-Grad, Essence, P-2500 P-1010(B), P-2010(B), P-1524CP, P-2024(B), Series 2, Series 3, Constant Pressure, Flash 2000 P-010(S), Series 1 P-1040(B), P-2040(B), P-2100 (B), P-1100, Series 2, 3, Flash 2000, Prep 100 P-040(S), Series 1 PUMP HEAD MODEL L HEAD M HEAD MM HEAD H HEAD MH HEAD FOR NEXT GENERATION PUMPS: PBD-LST05(S/B), PB-LST05(S/B), P-LL05(S/B), P-LST05(S/B), P-LS05(S/B), P-CP12S P-ML10(S/B), P-MX10(S/B), P-MXT10(S/B), P-MXT10B-MS, P-M110(S/B) P-ML40(S/B), P-MX40(S/B), P-MXT40-(S/B), P-M140(S/B) PBD-LST10(S/B), PB-LST10(S/B), P-LL10(S/B), P-LST10(S/B), P-LS10(S/B), P-LD12(S/B), P-LD36(S/B), P-CP24S PBD-LST40(S/B), PB-LST40(S/B), P-LL40(S/B), P-LST40(S/B), P-LS40(S/B), P-LD100(S/B), P-PR100(S/B), P-CP100S, P-0100F(B) Piston assembly, ea P-1597 P-1097 P-0101 P-1198 P-1298 Standard piston seal kit, ea P-1595 P-1095 P-0103 P-1096 P-1295 Standard piston seals, 10/pk P-1591 P-1090 P-3003 Biocompatible piston seal kit*, ea P-1595B P-1095B P-0103B P-1096B P-1295B Piston seal kit, Organic, ea P-1599 P-1099 P-0109 P-3009 P-1299 Biocompatible Piston Seal Kit, Organic, ea P-1099B P-0109B Check valve SS (inlet & outlet) P P-1085 P P-1085 P Check valve PEEK kit (inlet & outlet) P-1086 P-1086 P-1086 P-1086 P-1086 Replacement capsules, SS (10/pk) P-1075 P-1075 P-1075 P-1075 P-1075 Replacement capsules, PEEK (10/pk) P-1076 P-1076 P-1076 P-1076 P-1076 Self flush check valve kit P-1084 P-1084 P-1084 Self flush assembly, SS P-1082 P-1081 Self flush assembly PEEK P-1088 P-1089 Inlet filter assy (fitting & element) Inlet filter element, 20 µm (2/pk) P-1170 P-1170 P-1170 P-1170 P-1170 Replacement outlet filter element, SS (2/pk) P-1175 P-1175 P-1175 P-1172 P-1172 Replacement outlet filter elements for PEEK P-1171 P-1171 P-1171 P-1171 P-1171 Pumps, ea Prime purge valve rebuild kit, SS P-1177 P-1177 P-1177 P-1177 P-1177 Prime purge valve rebuild kit, PEEK P-1178 P-1178 P-1178 P-1178 P-1178 Prime purge valve assy, SS, ea Prime purge valve assy, PEEK, ea Pulse dampener w/transducer, SS for P-1061 P-1061 P-1061 P-1061 P-1061 models with real time pressure monitoring Pulse dampener w/transducer, PEEK for P-1062 P-1062 P-1062 P-1062 P-1062 models with real time pressure monitoring Pulse dampener (no transducer), SS P-1065 P-1065 P-1065 Pulse dampener (no transducer), PEEK P-1066 P-1066 P-1066 Pulse dampener rebuild kit P-1063 P-1063 P-1063 P-1063 P-1063 *Seal kits include: Self flush seal, O-ring, primary seal, removal tool TECH TIP Seal Options Standard Seals Ultra High Molecular Weight Polyethylene Organic seals Graphite-filled PTFE for use with harsh chemicals NEW! Biocompatible Seal Option Now offering biocompatible seals which contain a fluoropolymer energizer (vs the Stainless Steel Energizer) 330 your chromatography specialists

6 /SSI Parts LC INSTRUMENT REPLACEMENT PARTS Alltech Pump Replacement Parts ALLTECH PUMP REPLACEMENT PARTS ALLTECH PUMP MODELS ALLTECH CHROM TECH NEXT GEN REPL PUMP SEAL KIT CHECK VALVE KIT PISTON Alltech 301 SS Pump, 10 ml/min P-LS10S P-1095 P-1085 P-1097 Alltech 301 SS Pump, 40 ml/min P-LS40S P-1096 P-1085 P-1198 Alltech 301 PEEK Pump, 10 ml/min P-LS10B P-1095B P-1086 P-1097 Alltech 301 PEEK Pump, 40 ml/min P-LS40B P-1096B P-1086 P-1198 Alltech 426 SS Pump, 10 ml/min P-LST10S P-1095 P-1085 P-1097 Alltech 426 SS Pump, 40 ml/min P-LST40S P-1096 P-1085 P-1198 Alltech 426 PEEK Pump, 10 ml/min P-LST10B P-1095B P-1086 P-1097 Alltech 426 PEEK Pump, 40 ml/min P-LST40B P-1096B P-1086 P-1198 Alltech 626 SS Pump, 12 ml/min P-LD12S P-1595 P-1085 (single cap) P (double cap) Alltech 626 PEEK Pump, 12 ml/min P-LD12B P-1595B P-1086 P-1597 Alltech 627 SS Pump, 100 ml/min P-PR100S P-1096 P-1085 P-1198 Alltech 627 PEEK Pump, 100 ml/min P-PR100B P-1096B P-1086 P-1198 Check Valves NEW! P-1597 Biocompatible Seals Now offering biocompatible seals which contain a fluoropolymer energizer (vs the Stainless Steel Energizer) P-1085 P-1087 P-1088 P-1075 P-1076 P-1086 Filter Elements P-1170 P-1172 P-1175 Prime Purge Valve Rebuild Kits P-1177 Other Pump Supplies MISCELLANEOUS SUPPLIES FOR CHROM TECH PUMPS DESCRIPTION REPLACEMENT LOW PRESSURE INLET TUBING & FITTINGS T /8" OD FEP Fluoropolymer tubing, 1/16" ID, 10' length XP-301 1/4-28 Flat-Bottom Delrin Nut & ETFE Ferrule (P-300), for 1/8" OD tubing, ea HIGH PRESSURE OUTLET TUBING & FITTINGS FOR 1/16" OD TUBING /16" OD PEEK tubing, 0.010" ID, 5' length U-106 1/16" OD SS tubing, 0.010" ID, 5' length U , SS Nut for 1/16" OD Tubing, ea U-401X SS Ferrule for 1/16" OD Tubing, 10/pk , PEEK Nut for 1/16" OD Tubing, ea SS Ferrule for 1/16" OD Tubing, 10/pk U-400/U

7 LC INSTRUMENT REPLACEMENT PARTS Agilent Parts & Supplies Agilent 1260 Infinity Isocratic LC Supplies 332 AGILENT 1260 INFINITY ISOCRATIC LC SUPPLIES PUMP SUPPLIES DESCRIPTION G Purge Valve Long with PTFE Frit PTFE frits, 5/pk G Passive inlet valve G Cartridge for active inlet valve, 600 bar G Valve, outlet, 600 bar Seal cap Sapphire piston Pump seal (reversed-phase) 2/pk Wash seal (reversed-phase) Seal keeper Wash seal gasket, 6/pk Ergonomic solvent reservoir, 1 L Ergonomic solvent reservoir, amber, 1 L G Bottle head assembly Glass filter, solvent inlet, 20 μm pore size Frit adaptor, PTFE, 3 mm, 4/pk A-Line Stay Safe cap (GL45), 1-port for 3.2 mm OD tubing. Includes vent valve with time strip. AUTOSAMPLER SUPPLIES μl loop for G1329A/B Rotor seal for injection valve (two position/six port 600 bar) Stator for injection valve (two position/six port 600 bar) G Needle assembly for G1329B G Standard needle seat, PEEK 0.17 mm ID capillary, 2.3 μl AGILENT TUBING, CONNECTIONS, & TOOLS Capillary/fitting starter kit, 0.17 mm ID Tubing, PEEK, 1.6 mm OD 0.18 mm ID 1.5 m G SS capillary, 400 x 0.17 mm, m/m, ps/ps G Capillary to metering device Tubing, stainless steel, 0.17 mm ID, 105 mm, no fittings Tubing, stainless steel, 0.17 mm ID, 150 mm, no fittings Tubing, stainless steel, 0.17 mm ID, 200 mm, no fittings Tubing, stainless steel, 0.17 mm ID, 280 mm, no fittings Tubing, stainless steel, 0.17 mm ID, 400 mm, no fittings Union, zero-dead-volume, stainless steel, no fittings Front and back ferrules, 1/16" SS fitting, 10/pk Finger-tight PEEK fittings, 1/16", 10/pk Finger-tight PEEK fittings, 1/16" mixed colors, 10/pk Tubing cutter, for plastic or PEEK tubing Rheotool socket wrench, 1/4" Multifunction tool AGILENT VIALS & CLOSURES Vial, screw top, amber with write-on spot, 2 ml, certified, 100/pk Vial, screw top, amber with write-on spot, 2 ml, certified, 1,000/pk Vial, screw top, clear, 2 ml, certified, 1,000/pk Cap, screw, blue, certified, PTFE/red silicone septum, 1,000/pk Cap, screw, blue, PTFE/red silicone septum, 100/pk Cap, screw, green, PTFE/white silicone septum, 100/pk your chromatography specialists (includes time strip) TECH TIP The capillary/fitting starter kit includes stainless steel capillaries (4 x 105 mm, 4 x 150 mm, 2 x 180 mm, 1 x 400 mm, 10 x 1/16" stainless steel fittings and ferrule, 10 x 1/16" PEEK finger-tight fittings, 10 x 1/16" colored PEEK fittings, 5' 1/16" PEEK tubing, (3) stainless steel unions, plastic tubing cutter, 1/4" Rheotool socket wrench, & multifunction tool / The new bottles feature an ergonomic design with a slimmer profile and ergonomically shaped gripping zones on both sides of the bottle, promoting easier and safer usage

8 Agilent Parts & Supplies LC INSTRUMENT REPLACEMENT PARTS Agilent Purge Valves AGILENT PURGE VALVES DESCRIPTION COMMENTS G Purge valve, long, with PTFE Frit For G1310, G1311, G1312, G1376, G2226 G Purge valve with PTFE frit For 1120 G Purge valve, short, with PTFE frit For 1120, 1220, G7110, G7111 pumps PTFE frit For G1310A, G1311A, G1312A, G1376A, G2226A, 1120, 1220,1220, G1310B, G1311B, G1312B, G1311C, G1312C, G4202A Seal cap Not suitable for outlet ball valves w/integrated seal G Bio-inert purge valve, long For 1260 bio-inert systems, G5611A position / 7 port rotor seal For G4220A, G4220B position / 7 port stator For G4220A, G4220B Multi purpose valve head For G4204A Stator 8pos/9port 1200 bar For G4204A Rotor for multi purpose valve head G4204A Stainless steel filter assembly with PEEK ring, 2 µm pore size For G1361A Open end wrench, 14 mm To remove purge valve G Agilent Inlet and Outlet Valves AGILENT INLET & OUTLET VALVES DESCRIPTION COMMENTS INLET VALVES G Active inlet valve without cartridge For G1310A, G1311A, G1312A, G1376A, G2226A, G1312B, G1312C Cartridge for active inlet valve 400 bar For G1310A, G1311A, G1312A, G1376A, G2226A, G1312C G Cartridge for active inlet valve 600 bar For G1312B G Bio-inert active inlet valve For G5611A G Bio-inert cartridge for active inlet valve, 600 bar For G5611A G Inlet valve for 1290 quaternary pump For G4204A G Valve assemblies (inlet/outlet) for prep pumps For G1361A G * Passive inlet valve For G1310B, G1311B, G1311C, 1120, 1220, G4302A G * Passive inlet valve For G4220A, G4220B Seal cap Not suitable for outlet ball valves w/integrated seal OUTLET VALVES G Outlet ball valve For G1310A, G1311A, G1312A, G1376A, G2226A, 1120, 1220, G1310B, G1311B G1311C, G1312B, G1312C, G4302A G Outlet ball valve For G4220A, G4220B, G4204A G Bio-inert outlet ball valve For G5611A G Valve assemblies (inlet/outlet) for prep pumps For G1361A Seal cap Not suitable for outlet ball valves w/integrated seal 1) Inlet valve with integrated seal 2) Outlet ball valve with integrated seal G G G G Authorized Distributor

9 LC INSTRUMENT REPLACEMENT PARTS Agilent Parts & Supplies Agilent Pistons & Seals AGILENT PISTONS & SEALS DESCRIPTION COMMENTS PISTONS Sapphire plunger For G5611A, 4302A Sapphire plunger For G1310A, G1311A, G1312A, G1376A, G2226A, 1120, 1220, G1310B, G1311B, G1312B, G1311C, G1312C G Sapphire plunger For G1361A SEALS G Bio-inert piston seal For G5611A Piston seal polyethylene For G4220A, G4220B, G4204A Piston seal graphite filled PTFE (reversed-phase) (2/pk) Piston seal Polyethylene (normal phase) Piston seal For G1361A SPECIFIC PARTS FOR 1290 INFINITY LC PUMPS For G1310A, G1311A, G1312A, G1376A, G2226A, 1120, 1220, G1310B, G1311B, G1312B, G1311C, G1312C, G4302A For G1310A, G1311A, G1312A, G1376A, G2226A, 1120, 1220, G1310B, G1311B, G1312B, G1311C, G1312C, G4302A G Jet Weaver 35 µl100 µl For G4220A, G4220B, G4204A G Jet Weaver 380 µl For G4220A, G4220B, G4204A G Clamp for in-line filter For G4204A G Outlet filter 1290 quaternary pump µm in-line filter For G4204A µm replacement frit, 5/pk For , Outlet valve for flush pump Tubing kit 270 mm, 2/pk G Tubing kit 140 mm, 2/pk Shut-off valve For G4220A, G4220B, G4204A G Inline pressure relief valve kit For G4220A, G4220B Solvent selection valve bridge tube G Pressure relief valve For G4220A, G4220B G PEEK seal for inlet weaver assembly G Seal holder integrated for 1290 pump G Seal holder integrated for 1290 pump Sapphire Piston & Seals For G4204A For G4220A, G4220B For G4220A, G4220B Agilent Seal Wash AGILENT SEAL WASH DESCRIPTION COMMENTS Peristaltic pump cassette with silicone tubing Silicone tubing, 1 mm ID, 3 mm OD, 5 m Peristaltic pump with ChemSure tubing ChemSure tubing for peristaltic pump WASH SEAL AND WASH KEEPER Solvent Reservoir and Supplies For 1100/1200/1200 RRLC and 1260 For 1100/1200/1200 RRLC and Wash seal For 1100/1200/1200 RRLC, 1120 and 1260/ Seal keeper For 1100/1200/1200 RRLC and 1120 G Bio-inert seal keeper For G5611A Wash seal gasket, 6/pk For 1100/1200/1200 RRLC, 1120 and 1260/ Wash seal PE For 1290/1260/1220 G Support ring For 1290/1260/1220 G Support ring integrated for 1290 pump G Seal holder integrated for 1290 pump Agilent Solvent Reservoirs AGILENT SOLVENT RESERVOIRS DESCRIPTION L reservoir, clear L reservoir, amber NEW! ERGONOMIC SOLVENT RESERVOIRS ml reservoir, clear ml reservoir, clear, with cap ml reservoir, amber, with cap L reservoir, clear L reservoir, clear, with cap L reservoir, amber Identification Silicone ring (8/pk with 4 different colors) Sticker for solvent bottles, 100/pk For 1290 without seal wash For 1290/1260/ The new bottles feature an ergonomic design with a slimmer profile and ergonomically shaped gripping zones on both sides of the bottle, promoting easier and safer usage. 334 your chromatography specialists

10 Agilent Parts & Supplies LC INSTRUMENT REPLACEMENT PARTS Agilent Solvent Filters If the filter is in good condition, the solvent will freely drip out of the solvent tube (hydrostatic pressure). If the solvent filter is partially blocked, only very little solvent will drip out of the solvent tube. Caution: Small particles can permanently block the capillaries and valves of the module Always filter solvents Never use the module without solvent inlet filter Agilent Solvent Filters AGILENT SOLVENT FILTERS DESCRIPTION Glass filter, solvent inlet, 20 µm pore size Glass filter, solvent inlet, 40 µm pore size Glass filter, solvent inlet, 40 µm pore size Glass filter, solvent inlet, 40 µm pore size RECOMMENDED USE SOLVENT FILTER FRIT ADAPTER FRIT INLET I D (mm) TUBE O D (mm) Analytical scale, micro scale Frit adapter, PTFE, 3 mm, 4/pk Preparative LC Frit adapter, PTFE for 4.7 mm OD G Preparative LC Frit adapter, PTFE, 4 mm 7 4 G For G2258A Dual Loop Autosampler Solvent inlet filter, stainless steel Capillary and nano systems Agilent Frits & Adapters Frit adapter, PTFE for 3.2 mm OD G FRIT AGILENT FRITS & ADAPTERS DESCRIPTION G Solvent mixer, 1100 Series Solvent mixer, short, 200 µl G Frit adapter, PTFE, for 4.7 mm OD tubing G Frit adapter, PTFE, for 3.2 mm OD tubing, for G2258A Dual Loop Autosampler O-ring, Viton, 30 mm Stainless steel filter assembly with PEEK ring, 2 µm pore size Agilent Miscellaneous Capillaries MISCELLANEOUS CAPILLARIES (WITHOUT FITTING) MATERIAL ID (mm) LENGTH (mm) OD (mm) A* OD (mm) B* COLOR SS /16" 1/16" Red SS /16" 1/16" Red SS /16" 1/16" Red SS /16" 1/16" Red SS /16" 1/16" Red SS /16" 1/16" Red SS /16" 1/16" Green SS /16" 1/16" Green SS /16" 1/16" Green SS /16" 1/16" Green SS /16" 1/16" Green SS /16" 1/16" Green SS /16" 1/16" Green SS /16" 1/16" Green (Shown with fitting not included) Authorized Distributor

11 LC INSTRUMENT REPLACEMENT PARTS Agilent Parts & Supplies Agilent Vacuum Degassers A vacuum degasser is recommended for: Maximum sensitivity in the low UV wavelength range High injection precision High retention time reproducibility Flow rates below 0.5 ml/min 1200 Series Vacuum Degasser AGILENT VACUUM DEGASSERS DESCRIPTION COMMENTS G Tubing kit, degasser to pump, 4/pk, 30 cm pieces of tubing with screws and bushings For G1322A, G1379A/B Inlet tubing For Agilent 1260 Infinity Quaternary Pump VL Mounting tool for flangeless nut For G1322A, G1379A/B Plastic tubing cutter For G1322A, G1379A/B Tefzel ferrules and SS lock rings, 1/8", 10/pk For G1322A, G1379A/B PPS nuts, 1/8", 1/4-28 thread, 10/pk For G1322A, G1379A/B Union, 1/4-28 thread, polypropylene For G1322A, G1379A/B PTFE solvent tubing, 5 m, 1.5 mm ID, 3 mm OD For G1322A, G1379A/B Disposable syringes, 20 ml, 100/pk For G1322A Syringe adapter, 1/16" OD, 2" long For G1322A G Micro vacuum degasser tubing kit For G1379A Micro vacuum degasser tubing kit For G1379B G Online degasser accessory kit, Includes 8 screws, 8 bushings, 4 markers, tubing, syringe and syringe adapter For G1322A, G1379A/B Vacuum chamber (2 channel) For G1379A, G1379B Tubing kit for vacuum chamber For G1379A, G1379B Vacuum chamber replacement kit Includes two and one For G1379A, G1379B TECH TIP Vacuum Degasser Care To generally clean the vacuum degasser tubing, flush the system with isopropanol Flush the degasser with water after using buffers Speed solvent changes by drawing solvent through the degasser and tubing with syringe adapter kit Agilent Injection Valves AGILENT INJECTION VALVES VALVE DESCRIPTION USE WITH VALVE 2 position/6 port injection valve, 400 bar 2 position/6 port micro injection valve, 400 bar 2 position/6 port injection valve, 600 bar 2 position/6 port MBB injection valve, 400 bar 2 position/6 port ultra high pressure valve, 1200 bar *Includes seal and stator face G1313A, G1329A, G1367A/B, 1120 G1377A, G1389A G1329B, G1367C SL, G1367D SL Plus, G1367E, 1220 Infinity LC RHEBUILD KIT ROTOR SEAL MATERIAL ROTOR SEAL STATOR STATOR FACE Vespel Tefzel PEEK Vespel PEEK G2260A Peek * G4226A Vespel your chromatography specialists

12 Agilent Parts & Supplies LC INSTRUMENT REPLACEMENT PARTS Agilent Needles & Needle Seats The needle should be replaced when it becomes bent, burred or blunt, or when it is leaking or plugged. You should suspect a leak if you notice a trail of buffer crystals on the needle seat. The needle seat can become blocked if the sample contains particulates, as this is the first restriction that the sample experiences. If this occurs, try backflushing the needle seat capillary. AGILENT NEEDLES AND NEEDLE SEATS AGILENT AUTOSAMPLER G1313A, G1329A/B, 1120, 1220 Infinity LC (automated sampler only) G1313A, G1329A, 1120, 1220 Infinity LC (automated sampler only) G1313A, G1329A/B, 1120, 1220 Infinity LC (automated sampler only) NEEDLE ASSEMBLY DESCRIPTION COMPATIBLE WITH NEEDLE SEAT Standard autosampler G Standard, PEEK 0.17 mm ID capillary, 2.3 µl G Standard, PEEK 0.12 mm ID capillary, 1.2 µl G For use with PEEK seat G Standard, PEEK 0.17 mm ID capillary, 2.3 µl G µl upgrade G Standard, 0.17 mm ID capillary, 2.3 µl G G1389A Micro LC autosampler G Micro LC, 100 µm ID capillary, 1.2 µl G G1367A/B G1367C SL and G1367D SL Plus G1367E Well plate autosampler (green) Well plate autosampler (blue) Well plate autosampler (black) 1290/1260 Infinity LC autosampler Micro LC, 50 µm ID capillary, 0.3 µl G G Vespel, well plate autosampler 0.17 mm ID capillary, 2.3 µl G Vespel, well plate autosampler 0.12 mm ID capillary, 1.2 µl G bar, w/seat capillary 0.17 mm ID x 100 mm, 0.8 mm OD PEEK 600 bar, w/seat capillary 0.12 mm ID x 100 mm, 0.8 mm OD G PEEK 600 bar, w/seat capillary 0.12 mm ID x 100 mm, 0.8 mm OD G G G G G G1377A Micro well plate sampler G Micro w/seat capillary, 100 µm G Micro w/seat capillary, 75 µm G Micro w/seat capillary, 50 µm G G2258A Dual loop autosampler G Twin, dual loop autosampler G G2260A Prep autosampler G Prep autosampler, 0.5 mm ID, 20 µl G G4226A G4226A 1290/1260 Infinity LC autosampler 1290/1260 Infinity LC autosampler G Seat assembly, Vespel 0.12 mm, 1290 Infinity LC autosampler G G Ultra low dispersion G G5667A Bio-inert needle, 600 bar G Bio-inert assembly, 600 bar G G G G G G ACCESSORIES DESCRIPTION USE WITH G Seat adapter G1313A, G1329A, G1389A, G2260A, 1120 and 1220 Infinity LC Finger caps, for Autosampler Gripper, 15/pk G Tool for micro seat capillary mounting G1377A G1313A, G1329A, G1389A, G2260A, 1120 and 1220 Infinity LC Authorized Distributor

13 LC INSTRUMENT REPLACEMENT PARTS Agilent Parts & Supplies Agilent Metering Device Supplies Infrequently, the metering device seal and piston may need replacement if you see loss in injection volume precision or metering device leaking. AGILENT METERING DEVICE SUPPLIES PISTON DESCRIPTION USE WITH PISTON SEAL DESCRIPTION SEAL Sapphire piston, 40 µl Sapphire plunger, 100 µl G1367D, G1389A, G1377A, G4226A G1313A, G1329A/B, G1367A/B/C, G1367E Piston seal, 2 mm, for G1367D, G1389A, G1377A Piston seal for G4226A Piston seal graphite filled PTFE (reversed-phase), 2/pk Sapphire plunger, 100 µl G5667A Bio-inert piston seal G Sapphire piston, 900 µl G1313A, G1329A/B, G1367E Metering valve seal Piston, 5 ml G2258A G Piston seal Agilent Loop Capillaries AGILENT LOOP CAPILLARIES DESCRIPTION AGILENT AUTOSAMPLER PEEK, SS Clad, Bioinert, 100 µl G5667A G Stainless steel loop capillary, 100 µl G1313A G1329A/B Infinity LC G1367A/B/C G G4226A G1367E Stainless steel loop capillary, 900 µl G1329A/B G G2260A Loop capillary, 40 µl G1367D G G4226A G1367E G1377A G G1389A G Loop capillary, 20 µl G4226A G G1367E Loop capillary, 8 µl G1389A G G1377A G Loop capillary, 5 µl G2260A G G your chromatography specialists

14 Agilent Parts & Supplies LC INSTRUMENT REPLACEMENT PARTS Agilent Autosampler Trays AGILENT AUTOSAMPLER TRAYS DESCRIPTION FOR G1313A, G1329A/B, 1120,1220 INFINITY LC SAMPLERS G position tray for 2 ml vials G position tray for 2 ml vials, thermostattable G position tray for 2 ml vials G position tray for 6 ml vials G External vial tray for 17 vials (disposal position) G Disposal tube for external vial tray FOR G1367A/B/C/D/E, G2258A, G4226A G Well plate tray, 2 well plates, 10 vials (supports 50 mm plates) G Vial plate for 54 x 2 ml vials, 6/pk Vial plate for 15 x 6 ml vials FOR G1367A/B/C/D/E G position tray for micro vials Also for G4226A Plate for 27 Eppendorf Safe-lock tubes, 0.5/1.5/2mL G CHROM TECH RELATED PRODUCTS Agilent Certified Vials, page 18 NEW! Safer Agilent Laboratory Solvent Bottles AGILENT SOLVENT BOTTLES DESCRIPTION Solvent bottle, clear, 125 ml Solvent bottle, clear, 500 ml with cap Solvent bottle, amber, 500 ml with cap Solvent bottle, clear, 1000 ml Solvent bottle, clear, 1000 ml with cap Solvent bottle, amber, 1000 ml Identification Silicone ring (8/pk with 4 different colors) Sticker for solvent bottles, 100/pk The new bottles feature an ergonomic design with a slimmer profile and ergonomically shaped gripping zones on both sides of the bottle, promoting easier and safer usage. Authorized Distributor

15 LC INSTRUMENT REPLACEMENT PARTS Agilent Parts & Supplies Agilent Fraction Collector Supplies Agilent fraction collectors are designed to process data in real-time for instantaneous and precise fraction collection, while increasing throughput on your purification system. So you can be certain that you are getting the highest degree of recovery and purity for your fractions even with low flow rates. Agilent Collecting Tubes & Trays AGILENT COLLECTING TUBES & TRAYS TRAY HOLE DIAMETER (mm) NO OF TUBES TUBE DIMENSIONS TUBE QTY G x 100mm /pk 30 x 48 mm /pk G x 100 mm /pk G x 100 mm /pk 16 x 48 mm /pk G x 100 mm /pk 12 x 48 mm /pk G Funnel tray 40 Any size G Agilent Well Plate Trays AGILENT WELL PLATE TRAYS TRAY DESCRIPTION WELL PLATE DESCRIPTION UNIT G Tray for 4 well plates, cooled G Tray for 4 well plates, adjustable, cooled G Tray for 2 well plates, 10 funnels, cooled Tray for 2 well plates, 10 vials, 2 ml well plates, 0.5 ml, polypropylene 120/pk well plates, 0.5 ml, polypropylene 10/pk deep well plates, 1 ml, polypropylene 50/pk Closing mats for 96-well plates, silicone, pre-slit, fits 96-well plates P/N and only 50/pk well plates, 90 µl, polypropylene 30/pk deep well collection plates with glass inserts, caps, and septa, pre-assembled, 0.35 ml Glass inserts, 350 µl 1000/pk Caps/septa for glass inserts 1000/pk well plates, 150 µl, conical, polypropylene 25/pk G Vial plate for 54 x 2 ml vials 6/pk Plate for 27 Eppendorf Safe-lock tubes, 0.5/1.5/2 ml Vial plate for 15 x 6 ml vials G Agilent Fraction Collector Capillary Kits & Needles AGILENT FRACTION COLLECTOR CAPILLARY KITS AND NEEDLES MODULE MAX FLOW RATE TUBE SIZE TUBING KIT NEEDLE LENGTH NEEDLE TYPICAL USE G1364B 100 ml/min 0.8 mm ID G G Tubes (max 100 mm) G1364C 1 ml/min 0.15 mm ID G mm G Tubes (max 48 mm), well plates, vials 10 ml/min 0.25 mm ID G mm G ml/min 0.25 mm ID G mm G Funnel tray (tubes max 75 mm) 100 ml/min 0.8 mm ID G mm G G1364D 4 µl/min 25 µm ID G MALDI targets, well plates 4-30 µl/min 50 µm ID G µl/min 100 µm ID G your chromatography specialists

16 Agilent Parts & Supplies LC INSTRUMENT REPLACEMENT PARTS Agilent Manual Injection Valves with Position Sensing Switches Agilent Manual Injection Valve Replacement Parts Rotor seals wear with use and need routine replacement Stators only need replacement if the ports are damaged PEEK rotor seals are incompatible with concentrated nitric and sulfuric acids AGILENT MANUAL INJECTION VALVES WITH POSITION SENSING SWITCHES DESCRIPTION COMMENTS position/ 6 port valve, 400 bar, for G1328A/B Manual FL-Injection Valve, 600bar position/ 6 port, 600 bar, for position/ 6 port valve, 600 bar, for Bio-inert position/ 6 port valve, 400 bar PEEK Prep, SST 400 bar, for 3725i Manual prep injection valve, PEEK ROTOR SEAL MATERIAL ROTOR SEAL STATOR FACE STATOR HEAD BEARING RING ISOLATION SEAL REPAIR KIT Analytical Tefzel Vespel PEEK Analytical PEEK NEEDLE PORT ADAPTER Analytical PEEK Bio PEEK Analytical Tefzel Preparative PEEK Preparative PEEK Agilent Thermostatted Column Compartment Supplies See Sample Loops (Page 271) AGILENT THERMOSTATTED COLUMN COMPARTMENT SUPPLIES DESCRIPTION RapID Resolution High Throughput capillary kit Used for converting an Agilent 1200 instrument to the RRLC configuration, to enable use of high efficiency columns (to 600 bar). Can also be used for Agilent 1100 instruments. G capillary kit for 0.12 mm ID G Low dispersion heat exchanger, double, 1.6 µl, for G1316C G High temperature heat exchanger, 1.6 µl, 0.12 mm ID, "R" G High temperature heat exchanger, 1.6 µl, 0.12 mm ID, "L" G Heat exchanger/cooler, 1.5 µl, 0.12 mm ID G Carrier for heat exchanger 1290 Infinity TCC and 1200 Series TCC SL Column Identification Module (CIM), 3/pk Column clamp, 6/pk Column holder for micro LC columns G Column holder clips (lamella type), 2/pk G Column connecting capillary with fittings, 7 cm, 0.12 mm ID, 1/16" male/male G Column connecting capillary with fittings, 9 cm, 0.17 mm ID, 1/16" male/male G Column connecting capillary with fittings, 18 cm, 0.12 mm ID, 1/16" male/male G Column connecting capillary with fittings, 18 cm, 0.17 mm ID, 1/16" male/male PEEK tubing, 1/32" OD, 0.4 mm ID, 450 mm, Micro valve to waste G Thermal column insulation enclosure kit G G G Authorized Distributor

17 LC INSTRUMENT REPLACEMENT PARTS Agilent Parts & Supplies Agilent Detector Supplies Agilent wavelength detectors combine exceptional flexibility with superior instrument control, data communication, and analytical capabilities. This section shows you how to maintain your detector's high level of selectivity and sensitivity. DETECTOR MAINTENANCE TIPS SYMPTOM WHAT TO DO ADDITIONAL INFORMATION Lamp does not ignite Exchange the lamp Perform a wavelength calibration test and an intensity test after lamp replacement Noise exceeds application limit Exchange the flow cell Perform a wavelength calibration test after flow cell replacement Drift exceeds application limit Exchange the lamp Perform a wavelength calibration test and a pressure tightness test after flow cell replacement Leaky flow cell (For G4212 only) Leaky flow cell (For all G1314/G1315/G1365 detectors) Lower intensity (For G4212 only) Lower intensity (For all G1314/G1315/G1365 detectors) Exchange the flow cell Clean or exchange the flow cell Exchange the flow cell Clean or exchange the flow cell Perform a wavelength calibration test after flow cell replacement Perform a wavelength calibration test and a pressure tightness test after flow cell replacement Perform a wavelength calibration test after flow cell replacement Perform a wavelength calibration test and a pressure tightness test after flow cell replacement Agilent Certified Lamps All lamps are tested for noise and drift specifications, correct operating voltage, light intensity and proper alignment Improved coating process increases Agilent lamp lifetimes up to 50% Agilent deuterium lamps are designed with a much narrower aperture providing increased light intensity and decreased noise translating into an appreciably higher signal-to-noise ratio By providing higher sensitivity, Agilent lamps can extend detection capabilities and improve qualification trace levels for more than 2,000 hours of use Agilent's lamps are manufactured in an ISO9001 certified environment and are fully traceable throughout every step of the production process. Each lamp is then tested to ensure it meets Agilent's performance specifications. Test equipment is regularly calibrated using optical standards certified by NIST (National Institute of Standards and Technology) or PTB (Physikalisch-Technische Bundesanstalt). AGILENT DETECTOR LAMPS DESCRIPTION COMMENTS VARIABLE WAVELENGTH DETECTOR (VWD) G Long life Deuterium lamp with RFID tag For G1314D/E/F and G7114A/B G Long life Deuterium lamp For G1314A/B/C, 1120 and 1220 Infinity LC DIODE ARRAY DETECTOR (DAD)/MULTIPLE WAVELENGTH DETECTOR (MWD) Long life HiS Deuterium lamp (8-pin) with RFID tag For G4212A/B and G7117A/B/C Long life Deuterium lamp with RFID tag For G1315C/D, G1365C/D, G7115A and G7165A 1220 LC with DAD Long life Deuterium lamp For G1315A/B and G1365A/B Long life Deuterium lamp For G1315A/B and G1365A/B * Deuterium lamp For G1315A/B and G1365A/B G Tungsten lamp For G1315A/B/C/D and G1365A/B/C/D *Standard lamp for 1000 hours of use only G your chromatography specialists

18 Agilent Parts & Supplies LC INSTRUMENT REPLACEMENT PARTS Agilent Variable Wavelength Detector (VWD) Supplies AGILENT VWD FLOW CELL SELECTION TYPICAL COLUMN LENGTH (cm) TYPICAL PEAK WIDTH RECOMMENDED FLOW CELL < = Micro Flow Cell High Pressure Flow ml/min Semi-micro Flow Cell Cell For Pressure Above 100 bar Standard Flow Cell > = Typical Flow Rate ml/min ml/min ml/min 1-2 ml/min ml/min Internal Column Diameter 1.0 mm Agilent Flow Cell and Repair Kits for VWD AGILENT FLOW CELL AND REPAIR KITS FOR VWD DESCRIPTION USE WITH SPECIFICATIONS REPAIR KIT G Standard flow cell, RFID G1314D/E/F 10 mm, 14 µl, 40 bar G G Standard "D" type flow cell G1314A/B/C 10 mm, 14 µl, 40 bar G G Semi-micro flow cell, RFID G1314D/E/F 6 mm, 5 µl, 40 bar G G Semi-micro flow cell G1314A/B/C 6 mm, 5 µl, 40 bar G G Micro flow cell, 3 mm, RFID G1314D/E/F 2 µl, 120 bar G G Micro flow cell, 3 mm G1314A/B/C 2 µl, 120 bar G G Micro flow cell, 5 mm G1314A/B/C 1 µl, 40 bar G G High pressure flow cell, RFID G1314D/E/F 10 mm, 14 µl, 400 bar G G High pressure flow cell G1314A/B/C 10 mm, 14 µl, 400 bar G Variable Wavelength Detector Agilent Capillaries for VWD AGILENT CAPILLARIES FOR VWD FLOW CELL FLOW CELL DESCRIPTION INLET CAPILLARY OUTLET CAPILLARY Standard flow cell, RFID Standard "D" type flow cell Semi-micro flow cell, RFID Semi-micro flow cell Semi-micro flow cell 3 mm, RFID Semi-micro flow cell 3 mm Semi-micro flow cell 5 mm High pressure flow cell, V WD, 10mm, 14ul,RFID High pressure flow cell, V WD, 10mm, 14ul,RFID G Inlet capillary, 0.17 mm ID, 600 mm long Waste capillary, PEEK, 0.25 mm ID G /16" finger-tight PEEK fitting, 2/pk G Inlet capillary, 0.12 mm ID, 400 mm long Waste capillary, PEEK, 0.25 mm ID G /16" finger-tight PEEK fitting, 2/pk G Inlet capillary, 0.12 mm ID, 310 mm long G G Inlet capillary, 0.12 mm ID 400 mm long G Inlet capillary, 0.17 mm ID, 380 mm long G G Outlet capillary, 0.17 mm ID, 120 mm long Outlet capillary, 0.17 mm ID, 120 mm long G Outlet capillary, 0.17 mm ID, 120 mm long G G G Authorized Distributor

19 LC INSTRUMENT REPLACEMENT PARTS Agilent Parts & Supplies Agilent Diode Array Detector (DAD)/Multiple Wavelength Detector (MWD) Supplies Agilent Flow Cell and Repair Kits for DAD/MWD AGILENT FLOW CELL AND REPAIR KITS FOR DAD/MWD DESCRIPTION USE WITH SPECIFICATION REPAIR KIT G Standard flow cell w/rfid tag G1315C/D, G1365C/D 10 mm, 13 µl, 120 bar G G Standard flow cell G1315A/B, G1365A/B 10 mm, 13 µl, 120 bar G Semi-micro flow cell, RFID G1315C/D, G1365C/D 6 mm, 5 µl, 120 bar G G Semi-micro flow cell G1315A/B, G1365A/B 6 mm, 5 µl, 120 bar G Micro flow cell, RFID G1315C/D, G1365C/D 3 mm, 2 µl, 120 bar G G Micro high-pressure flow cell G1315A/B, G1365A/B 6 mm, 1.7 µl, 400 bar G nl flow cell 10 mm, 50 bar G nl flow cell 6 mm, 50 bar G Preparative flow cell G1315A/B, G1365A/B 3 mm, 120 bar, SS G G Preparative flow cell 0.3 mm, 20 bar, quartz G Preparative flow cell 0.06 mm, 20 bar, quartz G Max-Light cartridge cell G4212A/B Infinity LC DAD 10 mm, 1.0 µl, 60 bar G Max-Light cartridge cell G4212A/B Infinity LC DAD 60 mm, 4.0 µl, 60 bar G Max-Light cartridge test cell Must be used to perform detector build-in tests G Max-Light ultra low dispersion flow cell G Max-Light High Dynamic Range (HDR) flow cell G4212A/B Infinity LC DAD 10 mm V(σ) = 0.6 µl G4212A/B Infinity LC DAD 3.7 mm, V(σ) =0.9 µl G G G Agilent Capillaries for DAD/MWD AGILENT CAPILLARIES FOR DAD/MWD FLOW CELL FLOW CELL DESCRIPTION INLET CAPILLARY OUTLET CAPILLARY Std flow cell w/rfid tag G Inlet capillary w/heat exchanger, 0.17 mm ID, 590 mm long G mm ID, 200 mm long G Std flow cell G Semi-micro flow cell w/rfid tag G DAD heat exchanger capillary, 0.17 mm ID, 310 mm long G mm ID, 200 mm long G mm ID, 200 mm long G Semi-micro flow cell G mm ID, 200 mm long G mm ID, 200 mm long G Micro flow cell w/rfid tag G DAD heat exchanger capillary, 0.12 mm ID, 310 mm long G mm ID, 200 mm long G mm ID, 200 mm long G Micro high-pressure flow cell G Inlet capillary w/heat exchanger, 0.12 mm ID, 290 mm long G mm ID, 200 mm long G CHROM TECH RELATED PRODUCTS 0.5 µm Poroshell 120 Particle Expanded Offering! Now available in three particle sizes: 1.9 µm (NEW!) 2.7 µm 4 µm JT-PP12755 JT-TF12752-CC1 Agilent InfinityLab Poroshell 120 LC Columns, page 60 Solvent Waste Container Kit, page 262 Agilent Certified Vials, page your chromatography specialists

20 Agilent Parts & Supplies LC INSTRUMENT REPLACEMENT PARTS Agilent Diode Array Detector (DAD)/Multiple Wavelength Detector (MWD) Supplies Agilent 80 nl & 500 nl Flow Cell Supplies AGILENT 80 n L AND 500 n L FLOW CELL SUPPLIES DESCRIPTION QTY Fitting screw 10/pk Double winged nuts and 1/32" ferrules 10/pk /32" ferrule and stainless steel lock ring, lite touch 10/pk Union adjustment tool 2/pk Universal ZDV union, stainless steel, no fittings 2/pk Open end wrench, 4 mm ea Agilent Flow Cells & Replacement Parts AGILENT FLOW CELLS AND REPLACEMENT PARTS DESCRIPTION COMMENTS 500 n L FLOW CELL AND REPLACEMENT PARTS G nl flow cell Contains quartz flow cell with 10 mm path length and 500 nl volume and connecting capillaries, max 50 bar pressure G Quartz cell body, 10 mm G Cell seal assembly. 500 nl G Fused silica/peek capillary, 100 µm ID, 30 cm long Inlet G Fused silica/peek capillary, 50 µm ID, 40 cm long Inlet G Fused silica/peek capillary, 100 µm ID, 12 cm long Outlet G Fused silica/peek capillary, 50 µm ID, 12 cm long Outlet 80 n L FLOW CELL AND REPLACEMENT PARTS G nl flow cell Contains quartz flow cell with 6 mm path length and 80 nl volume and connecting capillaries, max 50 bar pressure G Quartz cell body, 80 nl, 6 mm path length G Fused silica/peek capillary, 50 µm ID, 40 cm long Inlet G Fused silica/peek capillary, 50 µm ID, 12 cm long Outlet G Fused silica/peek capillary, 25 µm ID, 20 cm long Inlet G Fused silica/peek capillary, 25 µm ID, 60 cm long Outlet PREPARATIVE FLOW CELL AND REPLACEMENT PARTS G Preparative flow cell, 0.3 mm, 20 bar, quartz G Preparative flow cell, 0.06 mm, 20 bar, quartz G PTFE tubing, 0.8 mm ID, 2 m G PTFE tubing, 0.5 mm ID, 0.8 m G Cell housing /16" finger-tight PEEK fitting, 2/pk G Quartz body, 0.3 mm G Prep flow cell, stainless steel, 3 mm, 120 bar G Stainless steel connecting capillary, 0.5 mm, 250 mm Authorized Distributor

21 LC INSTRUMENT REPLACEMENT PARTS Agilent Parts & Supplies Agilent G1362A 1100/1200 Series Refractive Index Detector (RID) Supplies AGILENT G1362A 1100/1200 SERIES REFRACTIVE INDEX DETECTOR (RID) SUPPLIES DESCRIPTION G Interface tubing kit: Includes 1/8" ferrule, 1/3" nut, PTFE tubing G Interface capillary, 400 mm, 0.17 mm ID G Restriction capillary, 0.17 mm ID Agilent G1321A/B 1100/1200 Series Fluorescence Detector (FLD) Supplies AGILENT G1321A/B 110/1200 SERIES FLUORESCENCE DETECTOR (FLD) SUPPLIES DESCRIPTION Detector lamp G Flow cell, 8 µl, 20 bar G Flow cell, 4 µl G Cuvette kit, 8 µl, 20 bar: Includes tubing, stainless steel fitting, front and back ferrule, PEEK fitting, syringe needle and syringe CUT-OFF FILTER KIT: Corrugated tubing, polypropylene, 6.5 mm ID, 5 m PTFE tubing, FEP, 0.7 mm ID, 5 m /16" finger-tight PEEK fitting, 2/pk G Column connecting capillary with fittings, 380 x 0.17 mm /16" stainless steel front ferrule, 10/pk /16" stainless steel back ferrule, 10/pk /16" stainless steel fitting, 10/pk Fluorescence detector calibration sample, 1 g glycogen Open end wrench, 1/4 and 5/16" Glass syringe Syringe needle G your chromatography specialists

22 Agilent Parts & Supplies LC INSTRUMENT REPLACEMENT PARTS Agilent LC/MS Supplies & Chemicals AGILENT LC/MS SUPPLIES LC/MS SUPPLIES DESCRIPTION 6100 SERIES SINGLE QUADRUPOLE 6200 SERIES TOF 6300 SERIES ION TRAP 6400 SERIES TRIPLE QUADRUPOLE 6500 SERIES ACCURATE- MASS Q-TOF G Tested ESI nebulizer assembly G2427A ES nebulizer needle (original) replacement kit G Nebulizer (needle SST316 replacement) kit G Tested APCI nebulizer assembly G2428A APCI nebulizer needle replacement kit G Corona needle APCI/Multimode G Capillary cap, high temperature, 3.0 mm G Capillary, 0.5 mm ID, dielectric* Capillary, 0.6 mm ID, dielectric* G Capillary, 0.6 mm ID, resistive, fast polarity switching* Spring, canted coil, 0.25" ID, 0.53 mm /6" tee, low dead volume, stainless steel Syringe adapter /16" finger-tight PEEK fitting PEEK tubing Syringe, 1 ml, PTFE, fixed Luer lock G HED assembly (G6140A, G6460A, G6530A) Electron multiplier replacement horn G Replacement horn and dynode LC/MS CHEMICALS G ES/APCI positive ion performance standard G ESI+APCI LC demo sample G2425A APCI negative ion performance standard, 5 x 1 ml ampoules G ES/APCI positive ion performance standard G ES/APCI positive ion performance standard G ES-TOF reference mix, 6 x 2 ml ampoules G ES-TOF biopolymer reference standard kit G Flushing solvent *Dielectric capillary supports standard polarity switching only. Resistive capillary supports fast polarity switching. CHROM TECH RELATED PRODUCTS G µm Poroshell 120 Particle Expanded Offering! Now available in three particle sizes: 1.9 µm (NEW!) 2.7 µm 4 µm Agilent InfinityLab Poroshell 120 LC Columns, page 60 Agilent Certified Vials, page 18 Authorized Distributor

23 LC INSTRUMENT REPLACEMENT PARTS Agilent Parts & Supplies Agilent LC/MS Foreline Pump Supplies AGILENT LC/MS FORELINE PUMP SUPPLIES DESCRIPTION Oil mist filter kit for E1M18/E2M28 G Oil mist cartridge filter for MS Oil return kit Foreline pump (rotary pump) oil, Inland 45, 1 L, for E1M18/E2M Foreline pump (rotary pump) oil, 4 L, for E1M18/E2M Oil mist filter element for E2M KF25 clamp, stainless steel KF25 coseal (inside clamp) G6011A Quiet Cover MS for vacuum pump model: Agilent MS40+ G3199B Quiet Cover 2.0 for vacuum pump models: BOC Edwards E2M28, E2M18, E1M18 Adding foreline pump (rotary pump) oil, 4 L G6011A Quiet Cover for vacuum pump Removable oil tray Easy access to pump Agilent LC/MS Common Supplies AGILENT LC/MS COMMON SUPPLIES* COMMON PARTS DESCRIPTION G Universal inlet filter replacement kit, includes: inlet filter, flexible capillaries 105 mm, stainless steel capillary 130 x 0.12 mm, union, column connect capillary 150 x 0.12 mm Rotor seal, Tefzel, ph Rotor seal, Vespel, ph Spring, canted coil CLEANING SUPPLIES Abrasive mesh, 4000 grit G Capillary cleaning wire for dip tube Cleaning powder, dielectric capillary, Alconox Cloths, lint-free Cotton swabs, 100/pk GAS PURIFIERS BHT-4 BMT-4 RMSN-4 RMSN-2 TOOLS G LC/MS tool kit Big hydrocarbon trap, 1/4" fittings Big moisture trap, 1/4" fittings Big universal trap, 1/4" fittings Big universal trap, 1/8" fittings, Nitrogen G Nebulizer Adjustment Kit Plastic tubing cutter Open end wrench, 1/4 and 5/16" Wrench, 1/2 and 7/16" Needle nose pliers, pointed serrated jaws *These parts are common to all LC/MS systems 0.5 µm Poroshell 120 Particle CHROM TECH RELATED PRODUCTS Expanded Offering! Now available in three particle sizes: 1.9 µm (NEW!) 2.7 µm 4 µm Agilent InfinityLab Poroshell 120 LC Columns, page 60 Agilent Certified Vials, page your chromatography specialists

24 Agilent Parts & Supplies LC INSTRUMENT REPLACEMENT PARTS Agilent LC/MS Calibrant Mixes AGILENT LC/MS CALIBRANT MIXES DESCRIPTION G Calibrant, ES G2432A APCI/APPI calibrant solution, 100 ml G2431A ESI tuning mix for ion trap, 100 ml G ES-TOF tuning mix, 100 ml G APCI-L low concentration tuning mix 100 ml G MMI-L low concentration tuning mix, 100 ml LC/MS Calibrant Mix and Source Compatibility Matrix LC/MS CALIBRANT MIX AND SOURCE COMPATIBILITY MATRIX SOURCE 6100 SERIES SINGLE QUADRUPOLE* 6140A/6150B SINGLE QUADRUPOLE 6200 SERIES TOF 6300 SERIES ION TRAP 6400 SERIES TRIPLE QUADRUPOLE 6500 SERIES ACCURATE-MASS Q-TOF ESI G G G G2431A G G APCI G2432A G G G2432A G G APPI G2432A G2432A G G2432A G2432A 2 G MMI G2432A G G G2432A G G NanESI G G2431A G HPLC-Chip Cube G G G2431A 1 G G *G6110A, G6120A/B, G6130A/B 1) 5X dilution suggested 2) No autotune 3) ESI positive tune only 4) Calibration only NEW! Safer Agilent Laboratory Solvent Bottles Agilent's new solvent bottles have been redesigned for easier use and safer handling, lowering the risk of accidents in the lab. The new bottles feature an ergonomic design with a slimmer profile and ergonomically shaped gripping zones on both sides of the bottle, promoting easier and safer usage. A predefined labeling area compatible with self-adhesive bottle labels, which stay on when you need them to, and colorful silicone bottle tags mean that you can clearly and unambiguously identify solvent compositions. An easy to read durable white ceramic print layout clearly indicates the nominal volume of the bottle and allos you to quickly determine the rest contents of the bottle. AGILENT SOLVENT BOTTLES DESCRIPTION Solvent bottle, clear, 125 ml Solvent bottle, clear, 500 ml with cap Solvent bottle, clear, 1000 ml Solvent bottle, clear, 1000 ml with cap Solvent bottle, amber, 500 ml with cap Solvent bottle, amber, 1000 ml Identification Silicone ring (8/pk with 4 different colors) Sticker for solvent bottles, 100/pk Authorized Distributor

25 LC INSTRUMENT REPLACEMENT PARTS ASI Replacement Parts for HPLC Pumps All check valves are self-priming, no external priming valve/syringe required Designed to deliver superior flow stability and accuracy Crush proof cartridge makes routine maintenance quick and easy Pistons are engineered to insure a high quality surface finish Seals are designed for long life, wide range of solvent compatibility and optimum performance Whether they are check valves, pistons or pump seals, all ASI replacement parts are designed to exceed the manufacturers specifications. All parts are subjected to rigorous engineering analysis and quality standards to insure the parts you purchase provide maximum life and superior chromatographic performance. Replacement parts are available for most HPLC pumps. Optimize Technologies Replacement Parts for HPLC Instrumentation Optimax Universal Cartridge for Opti-Max Housings A single Opti-Max cartridge is interchangeable across multiple pump brands and can function in either the inlet of outlet position. The same Opti-Max cartridge will fit into Opti-Max housings for pump manufactured by Waters, Shimadzu, Agilent/HP, and many other OEM solvent delivery systems. SS Cartridge PEEK Cartridge CARTRIDGE MATERIAL OPTIONS (2/PK) DESCRIPTION 1/32" Cartridge fits in all housings that will take 1/16" and 1/8" cartridges. CARTRIDGE BODY BALL AND SEAT PEEK/PPS Ruby/Sapphire /16" Recommended: Microbore cartridge SS Ceramic /16" UHPLC Microbore cartridge PEEK Ceramic Microbore cartridge SS Ruby/Sapphire Microbore cartridge PEEK Ruby/Sapphire Single ball & seat cartridge, rated to 18,000 PSI SS Ceramic Double ball & seat cartridge, rated to 20,000 PSI SS Ceramic /8" Recommended: Standard cartridge SS Ceramic Standard cartridge PEEK Ceramic Standard cartridge SS Ruby/Sapphire Standard cartridge PEEK Ruby/Sapphire *We cannot guarantee the Opti-Max check valve cartridge will fit in OEM housings. Opti-Max cartridges must be used with Opti-Max housing for optimal results. Ceramic Ball & Seat Ruby Ball w/sapphire Seat TECH TIP Ceramic Ball & Seat Ruby Ball w/sapphire Seat Cartridge Material Recommendations For high mechanical strength, stainless steel cartridges are the best choice. Ceramic ball & seat sets have higher density, which can make the valve more responsive and help the ball seat faster in lighter solvents. For biocompatible HPLC systems with nonmetallic flow paths or high percentages of THF, use PEEK cartridges with ceramic balls & seats. Optimax Free Turn Housing Most Opti-Max check valve housings feature the Free-Turn design. To see if they are available for your system, look for the Free-Turn logo next to the Opti-Max listings for your HPLC. Optimax FREE TURN housings can be removed with the tubing still attached, accelerating check valve maintenance while reducing fitting wear. Place wrench on the hex nut and turn. The hex nut or thread ring will turn independently of the fitting connection. Continue to unscrew the thread ring until the entire check valve assembly is removed from the pump head. Discard old check valve cartridge and replace with new cartridge. Install entire assembly into pump head. 350 your chromatography specialists