InertSustainSwift. Achieving separations faster than ever before

Transcription

1 InertSustainSwift TM C8 Achieving separations faster than ever before

2 InertSustainSwift C8 HPLC Columns Increasing Efficiency, Throughput and Productivity InertSustainSwift C8 columns are designed to deliver rapid separations with symmetric peaks to minimize analysis time as much as possible. InertSustainSwift C8 is also ideal for LC-MS and LC-MS/MS methods which offer highly sensitive results and enables MS compatible buffers to be used due to the extremely inert silica gel. Physical Properties Silica : ES (Evolved Surface) Silica Gel Particle Size :.9 μm, μm, μm Surface Area : 0 m /g Pore Size : 0 Å ( nm) Pore Volume :.00 ml/g Bonded Phase : Octadecyl Groups End-capping : Complete Carbon Loading : 9.0 % USP Code : L ph Range :.0 to Benefits Rapid elution of samples in isocratic methods Rapid column equilibration Highly inert packing material results in less tailing of peaks for virtually any type of analytes Deliver extreme sensitivity analysis in LC-MS and LC-MS/MS methods with low column bleed Extreme resistance to low and high ph mobile phases Excellent stability to 00 % aqueous mobile phases Endlessly reproducible from column-to-column and batch-to-batch

3 InertSustainSwift C8 HPLC Columns Rapid Elution of Samples in Isocratic Methods The retentivity of InertSustainSwift C8 is weaker even comparing with a conventional C8 column, which makes it ideal for methods requiring maximum throughput and efficiency InertSustainSwift C8 InertSustain C8 Inertil ODS-SP Eluent Column Size Flow Rate Col. Temp. : 40 Detection : UV 4 nm Sample : A) CH OH B) H O A/B = 80/, v/v : μm, 0 x. mm I.D. : 0. ml/min :. Uracil. Toluene. Ethylbenzene 4. Propylbenzene. n-butylbenzene 6. n-amylbenzene Inertil ODS InertSustain C Inertsil ODS- Sample No.6, n-amylbenzene was used to determine the hydrophobic property of columns. As shown above, InertSustainSwift C8 delivers rapid elution of samples in isocratic methods.

4 InertSustainSwift C8 HPLC Columns Rapid Analysis of Dissolution Test of Formulations InertSustainSwift C8 is optimally bonded offering moderate retentivity of analytes without accumulating strong hydrophobic contaminants in the column. Most importantly, the optimized surface area (0 m /g) and carbon load (9.0 %) to the silica gel enables rapid elution of samples in isocratic methods which creates the column to be highly efficient for dissolution test of formulations. Column Size : μm, 0 x 4.6 mm I.D. Eluent : CH CN/H O/CH COOH = 00/00/, v/v/v Flow Rate :.0 ml/min Col. Temp. : 0 Inj.Vol. : 00 μl Detection : UV nm Sample :. Valsartan InertSustainSwift C8 The adsorption of peak leads to poor peak shape with longer analysis time. Other Brands C8 Column Rapid Column Equilibration When columns are not equilibrated enough in gradient methods, irreproducible retention time of analytes, baseline drift and lower sensitivity in LC-MS and LC-MS/MS methods can be experienced. When discussing about rapid analysis in gradient methods, reducing the column equilibration time is significantly important to inject the next sample to achieve total rapid analysis. As shown below, InertSustainSwift C8 provide rapid column equilibration. Gradient Time Program Equilibration for min Equilibration for 0 min Baseline Drift Organic Solvent (%) Equilibration Time minutes 0 minutes minutes minutes Full Column Equilibration InertSustainSwift C8 XBridge C8. Methamidophos. Acephate

5 InertSustainSwift C8 HPLC Columns Benefits of Highly Inert Packing Material InertSustainSwift C8 employs a highly inert packing material which provides pure hydrophobic interaction between analytes without generating any secondary interaction delivering rapid results. Poorly end-capped columns often show tailing of peaks due to the presence of silanols resulting in longer analysis time InertSustainSwift C8.9 μm, 0 x. mm I.D. Eluent Flow Rate Col. Temp. : 40 Detection : UV 0 nm Injection Vol : 0. μl Sample : A) CH CN B) mm K HPO 4 (ph 7.0, KH PO 4 ) A/B = 0/70,v/v : 0.4 ml/min : :Uracil :Pyridine :Phenol 4:Berberine chloride :Dextromethorphan Titan C8.9 μm, 0 x. mm I.D. L column ODS μm, 0 x. mm I.D. References Test compounds for detecting the silanol effect on the elution of ionized amines in reversed-phase LC Nobuo Tanaka, Kensuke Okusa, Yuki Suita, Yukio Otsuka, Mineo Tahara, Tohru Ikegami, Masayoshi Ohira, Masakazu Takahashi Journal of Separation Science, Volume Issue, Pages 48 8, February 0 The effectiveness of several basic compounds for testing silica-based stationary phases was reviewed by applying them to recent columns for reversed-phase HPLC. Most octadecylsilylated (C8) stationary phases, prepared as a base-deactivated material from high-purity silica gel with endcapping, provided excellent peak shape and column efficiency for the bases including benzylamine and amitriptyline that once caused problems and were subsequently employed for testing silanol activities. However, a cyclic tertiary amine, dextrometorphan, was eluted as an acceptable peak from only a few columns at neutral ph. Such a more sensitive probe is expected to contribute to further improvement of the stationary phase for reversed-phase HPLC.

6 InertSustainSwift C8 HPLC Columns Extreme Sensitivity Analysis in LC-MS and LC-MS/MS Methods Mobile phases for LC/MS and LC-MS/MS must be volatile. Appropriate buffers are formate and acetate, while phosphate buffers are not compatible. Poorly end-capped columns contain silanols which often negatively influences separations and peak shapes or sensitivity. Such columns should use high concentrated buffers to prevent poor peak shapes, however, it generally show low sensitivity and can contaminate the instrument quickly. As shown in the following experiment, InertSustainSwift C8 maintains excellent peak shapes and high sensitivity even at low concentration buffer which leads to less harm to the instrument. Effects of Buffer Concentration (Ammonium Acetate) on Peak Shapes using Basic Drugs O N O CH CH N N CH CH N S N CH CH Column size : μm, 0. mm I.D. Eluent : A) Ammonium Acetate in H O B) Ammonium Acetate in CH OH :A/B = 0/70,v/v Flow Rate : 0. ml/min Col. Temp. : 40 Detection : LC/MS/MS (ESI, Positive, MRM) Sample : Each 0. (μg / ml) Dibucaine (pka 8.) Promethazine (pka 9.) Q > Q Dibcaine : 4. > 7. (+) Promethazine : 87.0 > 86. (+) 0 mm (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) mm (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) mm (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) CAPCELLPAK MG lll C min.0 min.0 min (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) Hypersil GOLD C min.0 min.0 min (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) Zorbax Eclipse Plus C min.0 min.0 min (x00,000).0 4:4.>7.(+) 7:87.0>86.(+). (x00,000).0 4:4.>7.(+) 7:87.0>86.(+). (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) InertSustainSwift C min.0 min.0 min

7 InertSustainSwift C8 HPLC Columns Low Bleed for LC-MS and LC-MS/MS Compatibility HPLC column bleed is a major source of background signal in LC-MS, LC-MS/MS analyses. This phase bleed occurs when the bonded phase or unreacted reagent elutes from the column during the analysis. Generally, highly endcapped columns often show high column bleed in LC-MS, LC-MS/MS applications. As shown below, not only InertSustainSwift C8 is a highly end-capped column, but also provides low column bleed which makes it compatible for LC-MS, LC-MS/MS methods (x,000,000,000) TIC(+)@ YMC-Triart C8 Column Size : μm, 0 x. mm I.D. Eluent : A) 0. % HCOOH in H O B) 0. % HCOOH in CH CN A/B = 9/ - min - 0/00 min Blank 0/00 0. min - 9/ min - 9/,v/v InertSustainSwift C8 Detection : LC/MS (ESI, Positive, Mass Range: 0-000) Flow Rate : 0. ml/min Col. Temp. : Q SCAN : m/z min

8 InertSustainSwift C8 HPLC Columns Extreme Resistance to Low and High ph Mobile Phases Dissolution testing is a very important testing in the pharmaceutical industry, which is widely used in formulation development monitoring the manufacturing process and quality control. The dissolution method development generally includes a ph solubility profile of the drug substance. In these testing, from low to neutral ph dissolution media are generally used. However, low ph dissolution media tends to cause poor column lifetime due to the acid hydrolysis of the bonded phase. As shown below, InertSustainSwift C8 not only show high resistance to low ph but also to high ph media due to the usage of a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties. Low ph Resistance Test % Initial Retention Time ph.0 ph.0 Purging Solvent : % TFA (ph.0)/ch CN = 90/0, v/v Purging Solvent : 0. % TFA (ph.0)/ch CN = 90/0, v/v Column Temp. : 60 Sample : Naphthalene Testing Procedure ) Purging solvent is introduced into column. ) The column is then flushed with 0 % CH CN. ) Naphthalene is used to verify the % initial retention. Hours of Purging (h) High ph Resistance Test % Initial Retention Time ph 9.0 ph Purging Solvent : 0 mm TEA (ph 9.0)/CH OH = 70/0, v/v Purging Solvent : 0 mm TEA (ph )/CH OH = 70/0, v/v Column Temp. : 0 Sample : Naphthalene Testing Procedure ) Purging solvnet is introduced into column. ) The column is then flushed with 0 % CH OH. ) Naphthalene is used to verify the % initial retention. Hours of Purging (h)

9 InertSustainSwift C8 HPLC Columns Excellent Stability to 00 % Aqueous Mobile Phases When analyzing hydrophilic compounds under water rich mobile phase condition, once the pump is stopped, the hydrophobic bonded group pushes the aqueous mobile phase out off the pore in an irreversible fashion, in what has become known as the dewetting phenomenon. As shown in the following test, InertSustainSwift C8 demonstrates excellent stability to dewetting guaranteeing highly stable, reliable and reproducible chromatograms. Dewetting Test First Injection Column : μm, 0 x 4.6 mm I.D. Eluent : 00 % H O Flow rate :.0 ml/min Col. Temp. : 40 Detection : UV 4 nm Sample :.Cytosine.Uracil.Guanine 4.Thymine.Adenine InertSustainSwift C8 Second Injection Variation: 99.6 % Testing Procedure ) 00 % water is introduced into column over 60 minutes. ) Conduct analysis. ) Stop flow for minutes. 4) 00 % water is introduced again into column over 0 minutes. ) Stop flow for minutes again. 6) Conduct analysis. First Injection First Injection First Injection Second Injection Variation: 9.0 % Second Injection Variation: 64. % Second Injection Variation: 46.7 % Luna C8() XBridge C8 Hypersil BDS C8 As shown above, other columns are apparently not compatible with 00 % aqueous mobile phase as it showed a sudden loss of retention due to a dewetting phenomenon.

10 InertSustainSwift C8 HPLC Columns Endlessly Reproducible from Column-to-Column and Batch-to-Batch Rigorous quality control of physical properties and strict chromatographic tests for inertness, contribute to the production of InertSustainSwift C8 with an outstanding reproducibility and long column lifetime. To maintain precise product reproducibility, strict chromatographic tests for inertness, durability, theoretical plates and reproducibility of retention time are employed. Column : um, 0 x 4.6 mm I.D. Strong Basic Compound. Dextromethorphan Strong Acidic Compound. Brilliant Blue FCF Strong Chelating Compound. Hinokitiol Lot Tf =.8 Tf =.8 Tf =.44 Lot Tf =. Tf =.4 Tf =.48 Lot Tf =. Tf =. Tf =

11 InertSustainSwift C8 HPLC Columns Comparison of Performance By conducting the tests below, we can evaluate the degree of inertness of the column. To strictly evaluate all columns, the below mentioned samples were used, which are all known as strong adsorptive compounds. Strong Basic Compound Test Dextromethorphan and Berberine Chloride are strong basic compounds. Severe tailing can be confirmed when the packing material contains residual silanol groups. O O N OCH OCH H CO N H CH Column : μm, 0 x. mm I.D. Eluent : A) CH CN B) mm K HPO 4 (ph 7.0, KH PO 4 ) A/B = 0/70,v/v Flow Rate : 0. ml/min Col. Temp. : 40 Detection : UV 0 nm Injection Vol : μl Sample : :Uracil :Pyridine :Phenol 4:Berberine Chloride :Dextromethorphan 4:Berberine Chloride :Dextromethorphan Strong Acidic Compound Test Brilliant Blue FCF has three sulfonic groups in its chemical structure, severe tailing will occur when the surface of the packing material is slightly basic. - O S SO - SO - Column : μm, 0 x. mm I.D. Eluent : A) CH CN B) 0. % H PO 4 A/B = /7,v/v Flow Rate : 0. ml/min Col. Temp. : 40 Detection : UV 4 nm Injection Vol : μl Sample : :Brilliant Blue FCF :4-Ethylbenzoic acid :Salicylic acid N N + :Brilliant Blue FCF Strong Chelating Compound Test Hinokitiol is a strong chelating compound, which coordinately binds with the surface of residual trace metal impurities, resulting in severe tailing. However, the peak shape improves as the injection increases since the surface of the packing material of the adsorption active sites eventually become masked. O OH Column : μm, 0 x. mm I.D. Eluent : A) CH CN B) 0. % H PO 4 A/B = 40/60 Flow Rate : 0. ml/min Col. Temp. : 40 Detection : UV 4 nm Injection Vol : μl Sample : : β-thujaplicin (Hinokitiol) : β-thujaplicin (Hinokitiol)

12 0 0 InertSustainSwift C8 HPLC Columns Comparison of Performance Strong Basic Compound Test Strong Acidic Compound Test Strong Chelating Compound Test InertSustainSwift C8 : Uracil : Pyridine : Phenol 4: Berberine chloride : Dextromethorphan : Brilliant Blue FCF : 4-Ethylbenzoic acid : Salicylic acid : Hinokitiol Gemini NX-C Luna C8()

13 0 0 InertSustainSwift C8 HPLC Columns Comparison of Performance Strong Basic Compound Test Strong Acidic Compound Test Strong Chelating Compound Test InertSustainSwift C8 : Uracil : Pyridine : Phenol 4: Berberine chloride : Dextromethorphan : Brilliant Blue FCF : 4-Ethylbenzoic acid : Salicylic acid : Hinokitiol Hypersil GOLD C8 4. No peak XBridge C Zorbax Eclipse Plus C

14 InertSustainSwift C8 HPLC Columns InertSustainSwift C8 HPLC columns are Excellent for Tablet/Capsule Dissolution Test Bioequivalence Test Continent Uniformity Test LC-MS, LC-MS/MS Methods Appropriate for Peptide and Oligonucleotide samples as well Analytical Columns Cat No InertSustainSwift C8.9 µm.x0mm InertSustainSwift C8.9 µm.x00mm InertSustainSwift C8.9 µm.x0mm InertSustainSwift C8.9 µm.0x0mm InertSustainSwift C8.9 µm.0x00mm InertSustainSwift C8.9 µm.0x0mm InertSustainSwift C8 HP µm.x0mm InertSustainSwift C8 HP µm.x00mm InertSustainSwift C8 HP µm.x0mm InertSustainSwift C8 HP µm.x0mm InertSustainSwift C8 HP µm.0x0mm InertSustainSwift C8 HP µm.0x00mm InertSustainSwift C8 HP µm.0x0mm InertSustainSwift C8 HP µm.0x0mm InertSustainSwift C8 HP µm 4.6X0mm InertSustainSwift C8 HP µm 4.6x00mm InertSustainSwift C8 HP µm 4.6x0mm InertSustainSwift C8 HP µm 4.6x0mm 0 * End-fittings are /6 Parker style.

15 InertSustainSwift C8 HPLC Columns Analytical Columns Cat No InertSustainSwift C8 µm.0x 0mm InertSustainSwift C8 µm.0x 0mm InertSustainSwift C8 µm.0x 7mm InertSustainSwift C8 µm.0x00mm InertSustainSwift C8 µm.0x0mm InertSustainSwift C8 µm.0x0mm InertSustainSwift C8 µm.x 0mm InertSustainSwift C8 µm.x 0mm InertSustainSwift C8 µm.x 7mm InertSustainSwift C8 µm.x00mm InertSustainSwift C8 µm.x0mm InertSustainSwift C8 µm.x0mm InertSustainSwift C8 µm.x 0mm 0-88 InertSustainSwift C8 µm.x 0mm InertSustainSwift C8 µm.x 7mm InertSustainSwift C8 µm.x00mm 0-88 InertSustainSwift C8 µm.xmm InertSustainSwift C8 µm.x0mm InertSustainSwift C8 µm.x0mm 0-88 InertSustainSwift C8 µm.0x 0mm 0-88 InertSustainSwift C8 µm.0x 0mm 0-88 InertSustainSwift C8 µm.0x 7mm InertSustainSwift C8 µm.0x00mm InertSustainSwift C8 µm.0xmm 0-88 InertSustainSwift C8 µm.0x0mm InertSustainSwift C8 µm.0x0mm InertSustainSwift C8 µm 4.0x 0mm InertSustainSwift C8 µm 4.0x 0mm InertSustainSwift C8 µm 4.0x 7mm InertSustainSwift C8 µm 4.0x00mm 0-88 InertSustainSwift C8 µm 4.0xmm InertSustainSwift C8 µm 4.0x0mm InertSustainSwift C8 µm 4.0x0mm InertSustainSwift C8 µm 4.6x 0mm InertSustainSwift C8 µm 4.6x 0mm InertSustainSwift C8 µm 4.6x 7mm InertSustainSwift C8 µm 4.6x00mm InertSustainSwift C8 µm 4.6xmm InertSustainSwift C8 µm 4.6x0mm InertSustainSwift C8 µm 4.6x0mm * End-fittings are /6 Waters-compatible.

16 InertSustainSwift C8 HPLC Columns Analytical Columns Cat No InertSustainSwift C8 µm.0x 0mm InertSustainSwift C8 µm.0x 0mm InertSustainSwift C8 µm.0x 7mm InertSustainSwift C8 µm.0x00mm InertSustainSwift C8 µm.0x0mm InertSustainSwift C8 µm.0x0mm InertSustainSwift C8 µm.x 0mm InertSustainSwift C8 µm.x 0mm InertSustainSwift C8 µm.x 7mm InertSustainSwift C8 µm.x00mm InertSustainSwift C8 µm.x0mm InertSustainSwift C8 µm.x0mm InertSustainSwift C8 µm.x 0mm InertSustainSwift C8 µm.x 0mm InertSustainSwift C8 µm.x 7mm InertSustainSwift C8 µm.x00mm InertSustainSwift C8 µm.xmm InertSustainSwift C8 µm.x0mm InertSustainSwift C8 µm.x0mm InertSustainSwift C8 µm.0x 0mm InertSustainSwift C8 µm.0x 0mm InertSustainSwift C8 µm.0x 7mm InertSustainSwift C8 µm.0x00mm InertSustainSwift C8 µm.0xmm InertSustainSwift C8 µm.0x0mm InertSustainSwift C8 µm.0x0mm InertSustainSwift C8 µm 4.0x 0mm InertSustainSwift C8 µm 4.0x 0mm InertSustainSwift C8 µm 4.0x 7mm InertSustainSwift C8 µm 4.0x00mm 0-88 InertSustainSwift C8 µm 4.0xmm InertSustainSwift C8 µm 4.0x0mm InertSustainSwift C8 µm 4.0x0mm InertSustainSwift C8 µm 4.6x 0mm InertSustainSwift C8 µm 4.6x 0mm InertSustainSwift C8 µm 4.6x 7mm InertSustainSwift C8 µm 4.6x00mm 0-88 InertSustainSwift C8 µm 4.6xmm InertSustainSwift C8 µm 4.6x0mm InertSustainSwift C8 µm 4.6x0mm * End-fittings are /6 Waters-compatible.

17 InertSustainSwift C8 HPLC Columns Guard Columns for UHPLC Cat No Holder for Guard Columns for UHPLC - Guard Columns for UHPLC, Holder/Cartridge Set, Holder with pcs.x0 mm Cartridges, InertSustainSwift C8.9 µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, Holder with pcs.x0 mm Cartridges, InertSustainSwift C8.9 µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8.9 µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8.9 µm Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8.9 µm Guard Columns for UHPLC,.0x0 mm, pcs, InertSustainSwift C8.9 µm Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8 µm Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8 µm Guard Columns for UHPLC,.0x0 mm, pcs, InertSustainSwift C8 µm 80 Cartridge Guard Column Ei (Non-Metal) Cat# Holder for 0 mm, Cartridge Guard Column Ei Cartridge Ei Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm Cartridge Ei Holder/Cartridge Set, Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm Cartridge Ei Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm Cartridge Ei Holder/Cartridge Set, Holder with pcs 4.0x0 mm Cartridges, InertSustainSwift C8 µm Cartridge Ei Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm Cartridge Ei Replacement Cartridges, pcs.x0mm, InertSustainSwift C8 µm 0-88 Cartridge Ei Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0-88 Cartridge Ei Replacement Cartridges, pcs 4.0x0mm, InertSustainSwift C8 µm * End-fittings are /6 Waters-compatible.

18 InertSustainSwift C8 HPLC Columns Cartridge Guard Column E Cat No Holder for 0 mm, Cartridge Guard Column E Holder for mm, Cartridge Guard Column E Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0-88 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm Holder with pcs 4.0x0 mm Cartridges, InertSustainSwift C8 µm Holder with pcs.0x mm Cartridges, InertSustainSwift C8 µm Holder with pcs 4.0x mm Cartridges, InertSustainSwift C8 µm Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm Holder with pcs 4.0x0 mm Cartridges, InertSustainSwift C8 µm 0-88 Holder with pcs.0x mm Cartridges, InertSustainSwift C8 µm Holder with pcs 4.0x mm Cartridges, InertSustainSwift C8 µm Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0-88 Cartridge E Replacement Cartridges, pcs.x0mm, InertSustainSwift C8 µm Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm Cartridge E Replacement Cartridges, pcs 4.0x0mm, InertSustainSwift C8 µm 0-88 Cartridge E Replacement Cartridges, pcs.0xmm, InertSustainSwift C8 µm 0-88 Cartridge E Replacement Cartridges, pcs 4.0xmm, InertSustainSwift C8 µm Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm Cartridge E Replacement Cartridges, pcs.x0mm, InertSustainSwift C8 µm Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm Cartridge E Replacement Cartridges, pcs 4.0x0mm, InertSustainSwift C8 µm 0-88 Cartridge E Replacement Cartridges, pcs.0xmm, InertSustainSwift C8 µm Cartridge E Replacement Cartridges, pcs 4.0xmm, InertSustainSwift C8 µm * End-fittings are /6 Waters-compatible.

19 InertSustainSwift C8 HPLC Columns GL Cart Guard Column Cat No. * End-fittings are /6 Waters-compatible Holder for GL Cart Multipurpose Type GL Cart Holder/Cartridge Set, Holder with pcs 4.6x mm Cartridges, InertSustainSwift C8 µm GL Cart Replacement Cartridges, 0 pcs 4.6x mm, InertSustainSwift C8 µm 0-88 GL Cart Holder/Cartridge Set, Holder with pcs 4.6x mm Cartridges, InertSustainSwift C8 µm 0-88 GL Cart Replacement Cartridges, 0 pcs 4.6x mm, InertSustainSwift C8 µm Conventional Guard Column Cat No Guard Column.0xmm InertSustainSwift C8 µm Guard Column.0x0mm InertSustainSwift C8 µm Guard Column.xmm InertSustainSwift C8 µm Guard Column.x0mm InertSustainSwift C8 µm Guard Column.xmm InertSustainSwift C8 µm Guard Column.x0mm InertSustainSwift C8 µm Guard Column.0xmm InertSustainSwift C8 µm 0-88 Guard Column.0x0mm InertSustainSwift C8 µm 0-88 Guard Column 4.0x0mm InertSustainSwift C8 µm Guard Column 4.0xmm InertSustainSwift C8 µm 0-88 Guard Column 4.0x0mm InertSustainSwift C8 µm Guard Column 4.6xmm InertSustainSwift C8 µm 0-88 Guard Column 4.6x0mm InertSustainSwift C8 µm Guard Column.0xmm InertSustainSwift C8 µm Guard Column.0x0mm InertSustainSwift C8 µm Guard Column.xmm InertSustainSwift C8 µm Guard Column.x0mm InertSustainSwift C8 µm Guard Column.xmm InertSustainSwift C8 µm Guard Column.x0mm InertSustainSwift C8 µm Guard Column.0xmm InertSustainSwift C8 µm Guard Column.0x0mm InertSustainSwift C8 µm Guard Column 4.0x0mm InertSustainSwift C8 µm Guard Column 4.0xmm InertSustainSwift C8 µm Guard Column 4.0x0mm InertSustainSwift C8 µm Guard Column 4.6xmm InertSustainSwift C8 µm Guard Column 4.6x0mm InertSustainSwift C8 µm * End-fittings are /6 Waters-compatible.

20 InertSustainSwift C8 HPLC Columns Preparative Columns Cat No InertSustainSwift C8 µm 6.0x 0mm InertSustainSwift C8 µm 6.0x00mm InertSustainSwift C8 µm 6.0x0mm InertSustainSwift C8 µm 6.0x0mm InertSustainSwift C8 µm 7.6x 0mm InertSustainSwift C8 µm 7.6x00mm InertSustainSwift C8 µm 7.6x0mm InertSustainSwift C8 µm 7.6x0mm InertSustainSwift C8 µm 0x 0mm InertSustainSwift C8 µm 0x00mm InertSustainSwift C8 µm 0x0mm InertSustainSwift C8 µm 0x0mm InertSustainSwift C8 µm 4x 0mm InertSustainSwift C8 µm 4x00mm InertSustainSwift C8 µm 4x0mm InertSustainSwift C8 µm 4x0mm InertSustainSwift C8 µm x 0mm InertSustainSwift C8 µm x00mm InertSustainSwift C8 µm x0mm InertSustainSwift C8 µm x0mm * End-fittings are /6 Waters-compatible. Guard Columns for Preparative Columns Cat No InertSustainSwift C8 µm 6.0x 0mm Guard InertSustainSwift C8 µm 7.6x 0mm Guard InertSustainSwift C8 µm 0x 0mm Guard InertSustainSwift C8 µm 4x 0mm Guard InertSustainSwift C8 µm x 0mm Guard * End-fittings are /6 Waters-compatible. Cartridge Guard Columns for Preparative Columns Cat No Holder for Prep Guard Cartridge Prep Guard Cartridges, pcs, 7.6x0 mm, InertSustainSwift C8 µm Prep Guard Holder/Cartridge Set, Holder with pcs 7.6x0 mm Cartridges, InertSustainSwift C8 µm * End-fittings are /6 Waters-compatible.

21 InertSustainSwift C8 HPLC Columns Capillary Columns Cat No Capillary EX-NANO x 0mm InertSustainSwift C8 µm Capillary EX-NANO x0mm InertSustainSwift C8 µm Capillary EX-NANO x0mm InertSustainSwift C8 µm Capillary EX-NANO 7x 0mm InertSustainSwift C8 µm Capillary EX-NANO 7x0mm InertSustainSwift C8 µm Capillary EX-NANO 7x0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm Capillary EX 0.x 0mm InertSustainSwift C8 µm Capillary EX 0.x0mm InertSustainSwift C8 µm Capillary EX 0.x 0mm InertSustainSwift C8 µm Capillary EX 0.x0mm InertSustainSwift C8 µm Capillary EX 0.7x 0mm InertSustainSwift C8 µm Capillary EX 0.7x0mm InertSustainSwift C8 µm EX Micro Guard (Metal) 0.xmm InertSustainSwift C8 µm Capillary EX-NANO x 0mm InertSustainSwift C8 µm Capillary EX-NANO x0mm InertSustainSwift C8 µm Capillary EX-NANO x0mm InertSustainSwift C8 µm Capillary EX-NANO 7x 0mm InertSustainSwift C8 µm Capillary EX-NANO 7x0mm InertSustainSwift C8 µm Capillary EX-NANO 7x0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm Capillary EX 0.x 0mm InertSustainSwift C8 µm Capillary EX 0.x0mm InertSustainSwift C8 µm Capillary EX 0.x 0mm InertSustainSwift C8 µm Capillary EX 0.x0mm InertSustainSwift C8 µm Capillary EX 0.7x 0mm InertSustainSwift C8 µm Capillary EX 0.7x0mm InertSustainSwift C8 µm EX Micro Guard (Metal) 0.xmm InertSustainSwift C8 µm EX Micro Guard (Non-Metal) 0.xmm InertSustainSwift C8 µm * End-fittings are Valco /6 (0- UNF). * Valco / (6-40 UNF) end-fittings are available, indicate / when ordering.

22 The GL Sciences name, the GL Sciences logo and the following registered trademark or trademark are the property of GL Sciences Inc. Inertsil InertSustain InertSustainSwift All other trademarks or service marks are the property of their respective owners. The specification and the column type are subject to change without notice due to continual improvements.