Glass Columns for Laboratory and Pilot Scale Liquid Chromatography

Transcription

1 Glass Columns for Laboratory and Pilot Scale Liquid Chromatography 1

2 Content 1. ECO PLUS Glass Columns Highlights Characteristics Available Versions Ordering Information Column Coupler & Glass Body ECO Glass Columns Highlights Characteristics Available Versions Ordering Information Column Coupler & Glass Body BioCart Cartridge Columns Highlights Characteristics Available Versions Ordering Information Cartridge Ordering Information Cartridge Holder Ordering Information Packing Adaptor Classic Glass Columns Highlights Characteristics Available Versions Ordering Information Pilot Columns Highlights Characteristics Ordering information Extract of reference list Packing Techniques for Glass Columns Packing the column with rigid media Packing the column with soft gels Quality control...19 Questionnaire for Packing Service of Glass Columns...20 Questionnaire for Kronlab Glass Columns...21 Questionnaire for Kronlab Pilot Columns

3 1. ECO PLUS Glass Columns ECO PLUS is the multi-purpose glass column for almost all types of liquid chromatography application. Adjustable length plungers at both ends and a totally inert triple chevron sealing system support the complete spectrum of applications from classical normal phase and reversed phase chromatography right through to biochromatography. The Quick-Lock connection system makes it possible to open and seal the column simply and quickly. 1.1 Highlights Biocompatible Universal application Solvent resistant Height adjustable plunger at both ends Easy to use Low temperature version available in polyethylene 1.2 Characteristics Pressure limit: Wetted parts: Diameter: Length: Connections: Height adjustment: Seal: bar Borosilicate glass, AB-Version: Polyethylene and EPDM; SR-Version: PTFE and sintered glass or stainless steel 5mm, 10mm, 15mm, 25mm, 35mm, 50mm 125mm, 250mm, 500mm Eight ¼ 28 fittings (four for 1 / 16 and four for 1 / 8 capillaries). The column is supplied with two M6 adaptors to allow direct connection to any LC-system. Multi-variable plunger at each end, each providing over 7cm bed height adjustment with Quick-Lock bayonet system AB version: EPDM O-Ring SR version: PTFE triple chevron 3

4 1.3 Available Versions ECO PLUS series glass columns AB (aqueous buffer) Adjustable length plunger at both ends Temperature range: 4-40 ºC Plunger: Polyethylene Seal: O-Ring EDPM (ethylene polymer elastomer) Frit: ID 5-50mm: polyethylene (5 or 10 µm) ECO PLUS series glass columns SR (solvent resistant) Adjustable length plunger at both ends Temperature range: ºC Plunger: PTFE Seal: PTFE triple chevron Frit: ID 5-15mm: sinter glass (2 or 10 µm) ID 25-50mm: stainless steel (2 or 10 µm) 1.4 Ordering Information Internal Diameter [mm] Min bed length [mm] Max. bed length [mm] Min. [ml] Max. [ml] Pressure Limit [bar] Frit porosity [µm] AB SR , TAC05/125PE0-AB TAC05/125G0-SR , or 5 TAC05/125PE5-AB TAC05/125G2-SR ,2 4, TAC05/250PE0-AB TAC05/250G0-SR ,2 4, or 5 TAC05/250PE5-AB TAC05/250G2-SR , TAC05/500PE0-AB TAC05/500G0-SR , or 5 TAC05/500PE5-AB TAC05/500G2-SR TAC10/125PE0-AB-1 TAC10/125G0-SR or 5 TAC10/125PE5-AB-1 TAC10/125G2-SR TAC10/250PE0-AB-1 TAC10/250G0-SR or 5 TAC10/250PE5-AB-1 TAC10/250G2-SR TAC10/500PE0-AB-1 TAC10/500G0-SR or 5 TAC10/500PE5-AB-1 TAC10/500G2-SR TAC15/125PE0-AB-1 TAC15/125G0-SR or 5 TAC15/125PE5-AB-1 TAC15/125G2-SR TAC15/250PE0-AB-1 TAC15/250G0-SR or 5 TAC15/250PE5-AB-1 TAC15/250G2-SR TAC15/500PE0-AB-1 TAC15/500G0-SR or 5 TAC15/500PE5-AB-1 TAC15/500G2-SR TAC25/125PE0-AB-1 TAC25/125S0-SR or 5 TAC25/125PE5-AB-1 TAC25/125S2-SR TAC25/250PE0-AB-1 TAC25/250S0-SR or 5 TAC25/250PE5-AB-1 TAC25/250S2-SR TAC25/500PE0-AB-1 TAC25/500S0-SR or 5 TAC25/500PE5-AB-1 TAC25/500S2-SR TAC35/125PE0-AB-1 TAC35/125S0-SR or 5 TAC35/125PE5-AB-1 TAC35/125S2-SR TAC35/250PE0-AB-1 TAC35/250S0-SR or 5 TAC35/250PE5-AB-1 TAC35/250S2-SR TAC35/500PE0-AB-1 TAC35/500S0-SR or 5 TAC35/500PE5-AB-1 TAC35/500S2-SR-1 4

5 Internal diameter [mm] Min. bed length [mm] Max. bed length [mm] Min. [ml] Max. [ml] Pressure Limit [bar] Frit porosity [µm] AB SR TAC50/125PE0-AB-1 TAC50/125S0-SR or 5 TAC50/125PE5-AB-1 TAC50/125S2-SR TAC50/250PE0-AB-1 TAC50/250S0-SR or 5 TAC50/250PE5-AB-1 TAC50/250S2-SR TAC50/500PE0-AB-1 TAC50/500S0-SR or 5 TAC50/500PE5-AB-1 TAC50/500S2-SR Column Coupler & Glass Body ECO PLUS series packing adaptors consist of a column coupler and an empty glass body. These must be of the same diameter as the column to be packed but the length of the additional glass body used should be selected to allow: Double the of slurry compared to the required packed bed if packing silica materials. Three times the for softer packing materials. Internal Diameter Description Length Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions TAC05KU-AS TAC10KU-AS TAC15KU-AS TAC25KU-AS TAC35KU-AS TAC50KU-AS 5 Glass Body for use with Column Coupler 125 TAC05/125 5 Glass Body for use with Column Coupler 250 TAC05/250 5 Glass Body for use with Column Coupler 500 TAC05/ Glass Body for use with Column Coupler 125 TAC10/ Glass Body for use with Column Coupler 250 TAC10/ Glass Body for use with Column Coupler 500 TAC10/ Glass Body for use with Column Coupler 125 TAC15/ Glass Body for use with Column Coupler 250 TAC15/ Glass Body for use with Column Coupler 500 TAC15/ Glass Body for use with Column Coupler 125 TAC25/ Glass Body for use with Column Coupler 250 TAC25/ Glass Body for use with Column Coupler 500 TAC25/ Glass Body for use with Column Coupler 125 TAC35/ Glass Body for use with Column Coupler 250 TAC35/ Glass Body for use with Column Coupler 500 TAC35/ Glass Body for use with Column Coupler 125 TAC50/ Glass Body for use with Column Coupler 250 TAC50/ Glass Body for use with Column Coupler 500 TAC50/500 5

6 2. ECO Glass Columns ECO is a competitively priced glass column for almost all types of soft gel and low pressure liquid chromatography application. The choice of adjustable length plunger and seal composition support the complete spectrum of applications from classical normal phase and reversed phase chromatography right through to biochromatography. 2.1 Highlights 2 adjustable length plungers supplied on request Biocompatible Water- jacketed version available Solvent resistant Easy to use Low temperature application 2.2 Characteristics Pressure limit: Wetted parts: Diameter: Length: Connections: Height adjustment: Seal: bar Borosilicate glass, AB-Version: POM, sintered glass frit and Viton seal; SR-Version: PVDF, sintered glass frit and Kalrez seal 10mm, 15mm, 20mm, 25mm, 32mm, 50mm 120mm, 200mm, 450mm, 750mm, 1000mm ID mm Two ¼ 28 fittings for 1 / 16 capillaries ID mm: Two ¼ 28 fittings for 1 / 8 capillaries Adjustable plunger provides up to 120mm bed height adjustment (2 adjustable plunger on request). Viton or Kalrez O-Ring 6

7 2.3 Available Versions ECO series glass columns aqueous applications Single adjustable length plunger Temperature range: 4-40 ºC Plunger: POM Seal: Viton O-Ring Frit: porous glass ECO series glass columns solvent resistant Single adjustable length plunger Temperature range: ºC Plunger: PVDF Seal: Kalrez O-Ring Frit: porous glass 2.4 Ordering Information Internal Diameter Minimum bed length Maximum bed length Minimum (ml) Maximum (ml) Pressure Limit (bar) Frit Porosity (µm) Viton seal AB Kalrez seal SR ECO10/120V3V ECO10/120V3K ECO10/120V0V ECO10/120V0K ECO10/200V3V ECO10/200V3K ECO10/200V0V ECO10/200V0K ECO10/450V3V ECO10/450V3K ECO10/450V0V ECO10/450V0K ECO10/750V3V ECO10/750V3K ECO10/750V0V ECO10/750V0K ECO10/999V3V ECO10/999V3K ECO10/999V0V ECO10/999V0K ECO15/120V3V ECO15/120V3K ECO15/120V0V ECO15/120V0K ECO15/200V3V ECO15/200V3K ECO15/200V0V ECO15/200V0K ECO15/450V3V ECO15/450V3K ECO15/450V0V ECO15/450V0K ECO15/750V3V ECO15/750V3K ECO15/750V0V ECO15/750V0K ECO15/999V3V ECO15/999V3K ECO15/999V0V ECO15/999V0K ECO20/120V3V ECO20/120V3K ECO20/120V0V ECO20/120V0K ECO20/200V3V ECO20/200V3K ECO20/200V0V ECO20/200V0K ECO20/450V3V ECO20/450V3K ECO20/450V0V ECO20/450V0K ECO20/750V3V ECO20/750V3K ECO20/750V0V ECO20/750V0K ECO20/999V3V ECO20/999V3K ECO20/999V0V ECO20/999V0K continue... 7

8 Internal Diameter Minimum bed length Maximum bed length Minimum (ml) Maximum (ml) Pressure Limit (bar) Frit porosity (µm) Viton seal AB Kalrez seal SR ECO25/120V3V ECO25/120V3K ECO25/120V0V ECO25/120V0K ECO25/200V3V ECO25/200V3K ECO25/200V0V ECO25/200V0K ECO25/450V3V ECO25/450V3K ECO25/450V0V ECO25/450V0K ECO25/750V3V ECO25/750V3K ECO25/750V0V ECO25/750V0K ECO25/999V3V ECO25/999V3K ECO25/999V0V ECO25/999V0K ECO32/120V3V ECO32/120V3K ECO32/120V0V ECO32/120V0K ECO32/200V3V ECO32/200V3K ECO32/200V0V ECO32/200V0K ECO32/450V3V ECO32/450V3K ECO32/450V0V ECO32/450V0K ECO32/750V3V ECO32/750V3K ECO32/750V0V ECO32/750V0K ECO32/999V3V ECO32/999V3K ECO32/999V0V ECO32/999V0K ECO50/120V3V ECO50/120V3K ECO50/120V0V ECO50/120V0K ECO50/200V3V ECO50/200V3K ECO50/200V0V ECO50/200V0K ECO50/450V3V ECO50/450V3K ECO50/450V0V ECO50/450V0K ECO50/750V3V ECO50/750V3K ECO50/750V0V ECO50/750V0K ECO50/999V3V ECO50/999V3K ECO50/999V0V ECO50/999V0K 2.5 Column Coupler & Glass Body ECO series packing adaptors consist of a column coupler and an empty glass body. These must be of the same diameter as the column to be packed but the length of the additional glass body used should be selected to allow: Double the of slurry compared to the required packed bed if packing silica materials. Three times the for softer packing materials. Internal Diameter Description Length Column Coupler (no Glass Body) including Viton O-Ring Column Coupler (no Glass Body) including Viton O-Ring Column Coupler (no Glass Body) including Viton O-Ring Column Coupler (no Glass Body) including Viton O-Ring ECO10KU/V ECO15KU/V ECO20KU/V ECO25KU/V 8

9 32 50 Column Coupler (no Glass Body) including Viton O-Ring Column Coupler (no Glass Body) including Viton O-Ring ECO32KU/V ECO50KU/V 10 Glass Body for use with Column Coupler 120 ECO10/ Glass Body for use with Column Coupler 200 ECO10/ Glass Body for use with Column Coupler 450 ECO10/ Glass Body for use with Column Coupler 750 ECO10/ Glass Body for use with Column Coupler 1000 ECO10/ Glass Body for use with Column Coupler 120 ECO15/ Glass Body for use with Column Coupler 200 ECO15/ Glass Body for use with Column Coupler 450 ECO15/ Glass Body for use with Column Coupler 750 ECO15/ Glass Body for use with Column Coupler 1000 ECO15/ Glass Body for use with Column Coupler 120 ECO20/ Glass Body for use with Column Coupler 200 ECO20/ Glass Body for use with Column Coupler 450 ECO20/ Glass Body for use with Column Coupler 750 ECO20/ Glass Body for use with Column Coupler 1000 ECO20/ Glass Body for use with Column Coupler 120 ECO25/ Glass Body for use with Column Coupler 200 ECO25/ Glass Body for use with Column Coupler 450 ECO25/ Glass Body for use with Column Coupler 750 ECO25/ Glass Body for use with Column Coupler 1000 ECO25/ Glass Body for use with Column Coupler 120 ECO32/ Glass Body for use with Column Coupler 200 ECO32/ Glass Body for use with Column Coupler 450 ECO32/ Glass Body for use with Column Coupler 750 ECO32/ Glass Body for use with Column Coupler 1000 ECO32/ Glass Body for use with Column Coupler 120 ECO50/ Glass Body for use with Column Coupler 200 ECO50/ Glass Body for use with Column Coupler 450 ECO50/ Glass Body for use with Column Coupler 750 ECO50/ Glass Body for use with Column Coupler 1000 ECO50/999 9

10 3. BioCart Cartridge Columns BioCart is the multi-purpose glass cartridge column designed for medium and high pressure liquid chromatography applications. An adjustable length plunger and easily replaceable cartridges allows a wide variety of applications. The totally inert triple chevron sealing system allows applications from classical normal phase and reversed phase chromatography whilst the Viton O -ring sealing system allows aqueous buffers and cold room applications common to biochromatography. The quick connection system makes it possible to change the cartridge simply and without the need to unpack the cartridge. For usage of the BioCart cartridge one holder is essential. 3.1 Highlights Biocompatible Medium to high pressure application Solvent resistant Height adjustable plunger Easy to use Low temperature, aqueous buffer version available Choice of long (120mm) or short (60mm) adjustable plungers Packing adaptor available for user packing 3.2 Characteristics Pressure limit: Wetted parts: Diameter: Length: Connections: Height adjustment: Seal: bar Borosilicate glass, AB-Version: Polyethylene and Viton O-Ring; SR-Version: PTFE and stainless steel or sintered glass frit 5mm, 10mm, 15mm, 20mm, 25mm, 50mm 120mm, 260mm, 500mm ¼ 28 fittings for 1 / 16 to allow direct connection to any LC-system; ¼ 28 fittings for 1 / 8 (ID 50 mm) Adjustable plunger providing up to 12cm bed height adjustment. Quick release system without the need for tools AB version: Viton O-Ring SR version: PTFE triple chevron 10

11 3.3 Available Versions BioCart Glass Cartridges AB (aqueous buffer) Adjustable length plunger at one end Temperature range: 2-40 ºC Plunger: Polyethylene Seal: Viton O-Ring Frit: ID 5-50mm: polyethylene BioCart Glass Cartridges SR (solvent resistant) Adjustable length plunger at one end Temperature range: ºC Plunger: PTFE Seal: Frit: PTFE triple chevron ID 5-15mm: sinter glass ID 25-50mm: stainless steel Cartridge + Holder 3.4 Ordering Information Cartridge Internal Diameter Minimum bed length Maximum bed length Minimum (ml) Maximum (ml) Plunger type Pressure limit (bar) Frit porosity (µm) AB range (aqueous buffer) SR range (solvent resistant) long BC05/120L0-AB BC05/120L long 90 2 or 5 BC05/120L5-AB BC05/120L short BC05/120S0-AB BC05/120S short 90 2 or 5 BC05/120S5-AB BC05/120S long BC05/260L0-AB BC05/260L long 90 2 or 5 BC05/260L5-AB BC05/260L short BC05/260S0-AB BC05/260S short 90 2 or 5 BC05/260S5-AB BC05/260S long BC05/500L0-AB BC05/500L long 90 2 or 5 BC05/500L5-AB BC05/500L short BC05/500S0-AB BC05/500S short 90 2 or 5 BC05/500S5-AB BC05/500S long BC10/120L0-AB-1 BC10/120L long 80 2 or 5 BC10/120L5-AB-1 BC10/120L short BC10/120S0-AB-1 BC10/120S short 80 2 or 5 BC10/120S5-AB-1 BC10/120S long BC10/260L0-AB-1 BC10/260L long 80 2 or 5 BC10/260L5-AB-1 BC10/260L short BC10/260S0-AB-1 BC10/260S short 80 2 or 5 BC10/260S5-AB-1 BC10/260S long BC10/500L0-AB-1 BC10/500L long 80 2 or 5 BC10/500L5-AB-1 BC10/500L short BC10/500S0-AB-1 BC10/500S short 80 2 or 5 BC10/500S5-AB-1 BC10/500S2-1 11

12 Internal Diameter Minimum bed length Maximum bed length Minimum (ml) Maximum (ml) Plunger type Pressure limit (bar) Frit Porosity (µm) AB range (aqueous buffer) SR range (solvent resistant) long BC15/120L0-AB-1 BC15/120L long 70 2 or 5 BC15/120L5-AB-1 BC15/120L short BC15/120S0-AB-1 BC15/120S short 70 2 or 5 BC15/120S5-AB-1 BC15/120S long BC15/260L0-AB-1 BC15/260L long 70 2 or 5 BC15/260L5-AB-1 BC15/260L short BC15/260S0-AB-1 BC15/260S short 70 2 or 5 BC15/260S5-AB-1 BC15/260S long BC15/500L0-AB-1 BC15/500L long 70 2 or 5 BC15/500L5-AB-1 BC15/500L short BC15/500S0-AB-1 BC15/500S short 70 2 or 5 BC15/500S5-AB-1 BC15/500S long BC20/120L0-AB-1 BC20/120L long 60 2 or 5 BC20/120L5-AB-1 BC20/120L short BC20/120S0-AB-1 BC20/120S short 60 2 or 5 BC20/120S5-AB-1 BC20/120S long BC20/260L0-AB-1 BC20/260L long 60 2 or 5 BC20/260L5-AB-1 BC20/260L short BC20/260S0-AB-1 BC20/260S short 60 2 or 5 BC20/260S5-AB-1 BC20/260S long BC20/500L0-AB-1 BC20/500L long 60 2 or 5 BC20/500L5-AB-1 BC20/500L short BC20/500S0-AB-1 BC20/500S short 60 2 or 5 BC20/500S5-AB-1 BC20/500S long BC25/120L0-AB-1 BC25/120L long 50 2 or 5 BC25/120L5-AB-1 BC25/120L short BC25/120S0-AB-1 BC25/120S short 50 2 or 5 BC25/120S5-AB-1 BC25/120S long BC25/260L0-AB-1 BC25/260L long 50 2 or 5 BC25/260L5-AB-1 BC25/260L short BC25/260S0-AB-1 BC25/260S short 50 2 or 5 BC25/260S5-AB-1 BC25/260S long BC25/500L0-AB-1 BC25/500L long 50 2 or 5 BC25/500L5-AB-1 BC25/500L short BC25/500S0-AB-1 BC25/500S short 50 2 or 5 BC25/500S5-AB-1 BC25/500S long BC50/120L0-AB-1 BC50/120L long 35 2 or 5 BC50/120L5-AB-1 BC50/120L short BC50/120S0-AB-1 BC50/120S short 35 2 or 5 BC50/120S5-AB-1 BC50/120S long BC50/260L0-AB-1 BC50/260L long 35 2 or 5 BC50/260L5-AB-1 BC50/260L short BC50/260S0-AB-1 BC50/260S short 35 2 or 5 BC50/260S5-AB-1 BC50/260S long BC50/500L0-AB-1 BC50/500L long 35 2 or 5 BC50/500L5-AB-1 BC50/500L short BC50/500S0-AB-1 BC50/500S short 35 2 or 5 BC50/500S5-AB-1 BC50/500S2-1 12

13 3.5 Ordering Information Cartridge Holder Cartridge ID Cartridge Bed length Description BioCart Cartridge Holder short. BC05CH/S BioCart Cartridge Holder, long. BC05CH/L BioCart Cartridge Holder, superlong. BC05CH/SL BioCart Cartridge Holder short. BC10CH/S BioCart Cartridge Holder, long. BC10CH/L BioCart Cartridge Holder, superlong. BC10CH/SL BioCart Cartridge Holder short. BC15CH/S BioCart Cartridge Holder, long. BC15CH/L BioCart Cartridge Holder, superlong. BC15CH/SL BioCart Cartridge Holder short. BC20CH/S BioCart Cartridge Holder, long. BC20CH/L BioCart Cartridge Holder, superlong. BC20CH/SL BioCart Cartridge Holder short. BC25CH/S BioCart Cartridge Holder, long. BC25CH/L BioCart Cartridge Holder, superlong. BC25CH/SL BioCart Cartridge Holder short. BC50CH/S BioCart Cartridge Holder, long. BC50CH/L BioCart Cartridge Holder, superlong. BC50CH/SL 3.6 Ordering Information Packing Adaptor Includes cartridge holder for slurry container assembly; slurry container and coupling device Cartridge ID Cartridge Bed length Length of slurry container mm Description BioCart Packing Adaptor complete. BC05PA/SK BioCart Packing Adaptor complete. BC05PA/LK BioCart Packing Adaptor complete. BC05PA/SLK BioCart Packing Adaptor complete. BC10PA/SF BioCart Packing Adaptor complete. BC10PA/LF BioCart Packing Adaptor complete. BC10PA/SLF BioCart Packing Adaptor complete. BC15PA/SF BioCart Packing Adaptor complete. BC15PA/LF BioCart Packing Adaptor complete. BC15PA/SLF BioCart Packing Adaptor complete. BC20PA/SF BioCart Packing Adaptor complete. BC20PA/LF BioCart Packing Adaptor complete. BC20PA/SLF BioCart Packing Adaptor complete. BC25PA/SF BioCart Packing Adaptor complete. BC25PA/LF BioCart Packing Adaptor complete. BC25PA/SLF BioCart Packing Adaptor complete. BC50PA/SF BioCart Packing Adaptor complete. BC50PA/LF BioCart Packing Adaptor complete. BC50PA/SLF 13

14 4. Classic Glass Columns Classic columns meet the needs of today s chromatographers whilst anticipating future technology trends to higher performance stationary phases with more rigid gels and smaller particle sizes and higher back pressures. The adjustable length plunger and totally inert triple chevron sealing system makes this an ideal column for classic flash and normal phase chromatography. The Quick-Lock connection system makes it possible to open and seal the column simply and quickly. 4.1 Highlights For use with all rigid and soft high performance gels No dead due to PTFE chevron sealing technology Solvent resistant Height adjustable plunger Autoclavable Easy to use Conical tube design 4.2 Characteristics Pressure limit: Wetted parts: Diameter: Length: Connections: Height adjustment: Seal: bar Borosilicate glass, PTFE, stainless steel frit 30mm, 40mm, 60mm 220 mm, 340mm, 410mm, 660mm Two ¼ 28 fittings ( 1 / 8 capillaries). One adjustable plunger, over 6cm height adjustment Quick-Lock bayonet system PTFE triple chevron 14

15 4.3 Available Versions Classic series glass columns adjustable length plunger at one end- Temperature range: Plunger: Seal: Frit: 18-60ºC PTFE PTFE triple chevron stainless steel 4.4 Ordering Information Internal Diameter Bed length Volume (ml) Pressure Limit (bar) Pressure Limit (psi) Frit porosity (µm) Part No CL30/220S CL30/340S CL30/410S CL30/660S CL40/220S CL40/340S CL40/410S CL40/660S CL60/220S CL60/340S CL60/410S CL60/660S 15

16 5. Pilot Columns Pilot columns are designed for use in pilot and production scale applications with organic solvents (version SR) or aqueous buffers (version AB) which makes them applicable for all common methods in chromatography including normal phase, reversed phase or biochromatography. High precision CNC manufacturing of plungers and distribution plates provides outstanding flow distribution for a linear flow distribution profile. Precise and reliable bed height adjustment is achieved by hand wheel adjusters on the plunger. Versatility of mounting includes fixed stand, wheeled trolley or direct into plant. All materials are in compliance with FDA regulations, certification and full documentation is available for validation purposes. 5.1 Highlights biocompatible solvent resistant height adjustable plunger heating jackets available custom tailored for each application full stainless steel versions with extended pressure ratings available 5.2 Characteristics Pressure limit: Wetted parts: Diameter: Length: Column body: Connections: depending on column body borosilicate glass, PTFE, Polyethylene or stainless steel EPDM, Viton or Kalrez, seals, stainless steel or polyethylene frits 60mm, 100mm, 140mm, 200mm, 300mm, 450mm, 600mm (other on demand) 400mm, 750mm, others on demand borosilicate glass, acrylic glass or stainless steel ¼ tubing (stainless steel SR version or polyethylene - AB version) or larger, depending on diameter Height adjustment: Multi-variable plunger providing up to 400mm bed height adjustment. 16

17 5.3 Ordering information Internal Diameter Minimum Maximum bed length bed length Minimum (l) Maximum Volume (l) Pressure limit (bar) AB range (aqueous buffer) SR range (solvent resistant) ,0 1,1 30 PI60/400-AB PI60/400-SR ,0 2,1 30 PI60/750-AB PI60/750-SR ,0 3,1 8,7 PI100/400-AB PI100/400-SR ,8 5,9 8,7 PI100/750-AB PI100/750-SR ,0 6,2 6 PI140/400-AB PI140/400-SR ,4 11,5 6 PI140/750-AB PI140/750-SR ,0 12,5 4 PI200/400-AB PI200/400-SR ,0 23,5 4 PI200/750-AB PI200/750-SR ,0 28,3 1.5 PI300/400-AB PI300/400-SR ,7 53,0 1.5 PI300/750-AB PI300/750-SR ,0 63,6 1 PI450/400-AB PI450/400-SR ,7 119,3 1 PI450/750-AB PI450/750-SR ,0 113,1 1 PI600/400-AB PI600/400-SR ,0 212,1 1 PI600/750-AB PI600/750-SR Kronlab offers special solutions for individual needs regarding: Materials Design Size System integration Please fill in the questionnaire on page 22 for a tailored column design! 5.4 Extract of reference list Bayer AG (columns in validated processes), diameter 100, 200, 300 Bayer AG, Dr. Grosser; best distribution and repeatability HKI Jena, Dr. Gura Novartis AG, Switzerland, Mr. Kurt Auer, diameter 150, 200, 300 Genetics Institute, USA (validated process) Abbott GmbH (several diameter) Roche Diagnostics GmbH (several diameter) 17

18 6. Packing Techniques for Glass Columns 6.1 Packing the column with rigid media Introduce a few ml of solvent or buffer (see instructions on packing material) into the packing device, so that the lower frit is moistened and free from air bubbles. Next the slurry is shaken carefully until it has a uniform consistency and quickly poured into the packing device without introducing any air bubbles. The slurry container must not have any air bubbles at all in it. If necessary, it can be topped up with solvent. The packing device is then sealed and packed as quickly as possible using a pump: this means that the flow rate should be set at the pressure limit of the column, so if necessary packing is carried out at the pump s maximum flow rate. A narrow PEEK capillary at the column outlet may improve packing quality, as it will act like a backpressure regulator and prevent the slurry entering the column too quickly at first. Pumping must continue at least until a constant pressure is reached. The flow rate during packing should always be considerably higher (>20%) than the flow rate needed for later use. After packing, the filling tube is unscrewed. Care must be taken when opening the column outlet so that any remaining pressure is released completely. The piston is introduced carefully, without allowing any particles to get between the glass and the piston seal. The column is now re-attached to the pump, the pump is started at low pressure and the flow gradually increased to the pressure limit of the column. At this point dead may occur between the variable piston and the column bed, which can be removed by moving the variable piston towards the column bed. For this, the column must not be under pressure, i.e. the pump must be turned off and the column inlet opened. Next the column is conditioned with the relevant eluents and is ready to be used. 6.2 Packing the column with soft gels Only degassed and filtered solvents or buffers may be used when packing chromatography columns. The lower frit is dampened and covered with approx. 1cm solvent. Next the slurry is introduced carefully and quickly, ensuring that no air bubbles occur. The column outlet should be open while the column is being filled; the solvent can also be sucked from the column outlet with a peristaltic pump at the same time. When the slurry has all been poured in, the gel must be allowed to settle and the solvent sink to approx cm above the packing level in the gel bed. The gel bed must not be allowed to run dry. The column outlet is closed or the peristaltic pump stopped. Next the variable piston is inserted, without allowing particles to come between the seal and the column body. By turning the lock slowly, the piston can be moved towards the gel bed. At the same time, all the air above the gel bed should be forced out of the column inlet. It is essential that the gel bed is not compressed when moving the piston towards it. Now the column can be equilibrated with the appropriate buffer or solvent. 18

19 Dead can occur between the gel bed and the piston during normal use, but this can be removed by moving the piston inwards. 6.3 Quality control We recommend that you determine plate count and peak symmetry with a suitable (non-adsorbent) test substance after packing the column. By repeating this test frequently, the quality and durability of the packing material can be recorded efficiently. Amount of theoretical plates (N): N = 5.54 x (T 1 / W 1/2 )² T 1 : retention time (sec) W 1/2 : peak width (sec) at half peak height HETP = L / N L: column length in mm Peak symmetry (S): S = A / B A : peak width to the right of the peak median B : peak width to the left of the peak median 19

20 Questionnaire for Packing Service of Glass Columns Kronlab provides packing service for all commercially available packing materials: Each column is tested individually and will be supplied with a column inspection report. Available for all types of Kronlab s glass columns: ECO, ECO Plus, Classic and BioCart. Kronlab recommends high purity silica of YMC Europe. A complete overview of bulk packing materials for RP- & NP-chromatography can be requested from: YMC Europe GmbH Schöttmannshof 19 D Dinslaken Tel: +49 (0) 2064 / Fax: +49 (0) 2064 / info@ymc.de internet: Ask for your individual quotation of your prepacked glass column: column type: inner diameter : bed length : bed (ml): packing material: particle size distribution [µm]: pore size [ ]: solvents biocompatibility: Customer s address: name: company/institute: department: phone: fax: address: country: 20

21 Questionnaire for Kronlab Glass Columns YMC Europe GmbH, Kronlab Chromatography Technology, Schöttmannshof 19, D Dinslaken, phone: +49 (0) 2064 / 427-0, fax: +49 (0) 2064 / info@kronlab.com inner diameter : min. bed length : max. bed length : bed (ml): max. pressure (bar): column design: cylindrical conical sealing: O-Ring PTFE triple chevron biocompatibility: yes no frit material: PE Glass Stainless steel porosity in µm: o-ring material: EPDM Viton Kalrez environment: cold storage room Room temperature solvents: packing material: quotation for packing device required: yes no Customer s address: name: company/institute: department: phone: fax: address: country: 21

22 Questionnaire for Kronlab Pilot Columns YMC Europe GmbH, Kronlab Chromatography Technology, Schöttmannshof 19, D Dinslaken, phone: +49 (0) 2064 / 427-0, fax: +49 (0) 2064 / info@kronlab.com inner diameter : min. bed length : max. bed length : max. pressure (bar): material column body: Glass Acrylglass SS O-ring material: EPDM Viton Kalrez biocompatible: yes no frit material: Polyethylene SS Titanium porosity (µm): capillary connections: Polyethylene PTFE SS capillary outer diamter: 1/8 1/4 1/2 capillary connection with valves: yes no tempering jacket: yes no temperature range: packing device: yes no packing material: solvents: Customer s address: name: company/institute: department: phone: fax: address: country: 22