Agilent 9000 Intuvo GC Applications. Matthew Giardina, Ph.D. James McCurry, Ph.D. Rebecca Veeneman, Ph. D.

Transcription

1 Agilent 9000 Intuvo GC Applications Matthew Giardina, Ph.D. James McCurry, Ph.D. Rebecca Veeneman, Ph. D.

2 Summary of Intuvo Applications by Industry Environmental EPA Method 8270 Semivolatile Organic Compounds (SVOC) Ultra-Fast Total Petroleum Hydrocarbons (TPH) Energy and Chemical Ultra-Fast SimDis using ASTM D7798 Monocyclic Aromatic Purity using ASTM D7504 Sulfur in Light Petroleum Liquids Food Multiresidue Pesticide Analysis in Food Pharma Extractables and Leachables Consumer Products Longevity of Phthalates Fragrance Impurities using Intuvo with OpenLab Match/Compare 1

3 Summary of Intuvo Applications by Industry Environmental EPA Method 8270 Semivolatile Organic Compounds (SVOC) Ultra-Fast Total Petroleum Hydrocarbons (TPH) Energy and Chemical Ultra-Fast SimDis using ASTM D7798 Monocyclic Aromatic Purity using ASTM D7504 Sulfur in Light Petroleum Liquids Results Equivalency Food Multiresidue Pesticide Analysis in Food Pharma Extractables and Leachables Consumer Products Longevity of Phthalates Fragrance Impurities using Intuvo with OpenLab Match/Compare 2

4 Summary of Intuvo Applications by Industry Environmental EPA Method 8270 Semivolatile Organic Compounds (SVOC) Ultra-Fast Total Petroleum Hydrocarbons (TPH) Energy and Chemical Ultra-Fast SimDis using ASTM D7798 Monocyclic Aromatic Purity using ASTM D7504 Sulfur in Light Petroleum Liquids Food Multiresidue Pesticide Analysis in Food Resilience to Matrix and Difficult Compounds Pharma Extractables and Leachables Consumer Products Longevity of Phthalates Fragrance Impurities using Intuvo with OpenLab Match/Compare 3

5 Summary of Intuvo Applications by Industry Environmental EPA Method 8270 Semivolatile Organic Compounds (SVOC) Ultra-Fast Total Petroleum Hydrocarbons (TPH) Energy and Chemical Ultra-Fast SimDis using ASTM D7798 Monocyclic Aromatic Purity using ASTM D7504 Sulfur in Light Petroleum Liquids Low Level Detection Food Multiresidue Pesticide Analysis in Food Pharma Extractables and Leachables Consumer Products Longevity of Phthalates Fragrance Impurities using Intuvo with OpenLab Match/Compare 4

6 Summary of Intuvo Applications by Industry Environmental EPA Method 8270 Semivolatile Organic Compounds (SVOC) Ultra-Fast Total Petroleum Hydrocarbons (TPH) Energy and Chemical Ultra-Fast SimDis using ASTM D7798 Monocyclic Aromatic Purity using ASTM D7504 Sulfur in Light Petroleum Liquids Ease of Use Food Multiresidue Pesticide Analysis in Food Pharma Extractables and Leachables Consumer Products Longevity of Phthalates Fragrance Impurities using Intuvo with OpenLab Match/Compare 5

7 Summary of Intuvo Applications by Industry Environmental EPA Method 8270 Semivolatile Organic Compounds (SVOC) Ultra-Fast Total Petroleum Hydrocarbons (TPH) Energy and Chemical Ultra-Fast SimDis using ASTM D7798 Monocyclic Aromatic Purity using ASTM D7504 Sulfur in Light Petroleum Liquids Speed and High Boilers Food Multiresidue Pesticide Analysis in Food Pharma Extractables and Leachables Consumer Products Longevity of Phthalates Fragrance Impurities using Intuvo with OpenLab Match/Compare 6

8 Agilent 9000 Intuvo GC Equivalency to

9 Semivolatiles GC Methods GC (7890 & Intuvo) Injection volume 1 µl Inlet Split/Splitless 300 C Pulsed splitless 60 psi until 0.5 min Purge 50 ml/min at 0.5 min Septum purge switched flow mode 3mL/min Liner UI Splitless liner with a single taper and glass wool at the bottom Guard chip (Intuvo only) 60 C for 2 min, 20 C/min to 260 C, 6 C/min to 330 C hold min (standards) or min (soil extract) Column Agilent DB-5ms UI 30 m x 0.25 mm x 0.5 µm Flow 2 ml/min constant flow Column temperature 40 C for 2 min, 20 C/min to 260 C, 6 C/min to 330 C hold min (standards) or min (soil extract) MSD (5977) Transferline temperature 330 C Drawout plate 6 mm Ion source temperature 330 C Quadrupole temperature 200 C Scan 35 to 550 m/z Gain factor 1 Threshold 50 A/D samples 2 8

10 Chromatographic Comparison Which Is Intuvo? 8270 Calibration Standard 77 Acids/Bases/Neutrals (PAHs), 6 ISTDs Intuvo

11 Relative Retention Time Retention Time Comparison Relative Retention Time = RT target / RT ISTD Compound Number 10

12 Relative Retention Time Retention Time Comparison Relative Retention Time = RT target / RT ISTD Intuvo Compound Number Average relative retention time difference =

13 Response Factor Response Comparison Response Factor = (Area target * Concentration ISTD )/(Area ISTD * Concentration target ) Intuvo Compound Number Average response factor difference = 4.6% 12

14 Sulfur in Gasoline GC Methods GC (7890 & Intuvo) Injection volume 1 µl Inlet Split/Splitless 300 C Split 10:1 Septum purge flow 3mL/min Liner UI Splitless liner with a single taper and glass wool at the bottom Guard chip (Intuvo only) 300 C Column Agilent DB-1 30 m x 0.32 mm x 1 µm Flow 2 ml/min constant flow Column temperature 40 C for 0.71 min, 14.1 C/min to 250 C hold 1 min SCD (8355) Burner 800 C Base 280 C (7890: 250 C) Oxidizer 50 ml/min (7890: 60 ml/min) Upper H 2 38 ml/min (7890: 40 ml/min) Lower H 2 8 ml/min Ozone 36 ml/min Range (stand alone) 6 13

15 Light Petroleum Sulfur Analysis A 20ppb standard containing 23 compounds was evaluated with a 10:1 split. Most of the analytes can be differentiated from the baseline at 20ppb making the practical limit of detection 2ppb. pa FID1B, BackSignal (AQM-SCD\SCD_160503\20pb_23S_iso_ D) pa Intuvo , SCD1 A, Front Signal (Chromatogr..2_DB1-1um_ \DB1_1um_O \20ppb-july_19oct2.D) , min # Analyte SNR (20ppb) 1 ethanethiol dimethyl sulfide carbon disulfide propanethiol methyl-2-propanethiol propanethiol ethylmethyl sulfide butanethiol thiophene coleution 10 2-methyl-1-propanethiol diethyl sulfide butanethiol dimethyl disulfide methylthiophene methylthiophene chlorothiophene bromothiophene diethyl disulfide di-tert-butyl disulfide thianaphthene methylbenzothiophene methylbenzothiophene diphenyl sulfide ISTD min 14

16 Active Compounds Resilience to Matrix Low Level Detection 15

17 Normailzed Response Semivolatiles Suitability Standard Test system acid/base activity, inertness and MSD tuning ratios (25 µg/ml) Pentachlorophenol DFTPP Benzidine DDT Criteria: IE fragments within specified ion ratios Criteria: TF < 10% height Criteria: TF < 10% height Criteria: Breakdown to DDD and DDE < 20% Time (min) 16

18 Normailzed Response Semivolatiles Suitability Standard Test system acid/base activity, inertness and MSD tuning ratios (25 µg/ml) Pentachlorophenol DFTPP Benzidine DDT Pass Ion ratios in spec Pass TF = Pass TF = 1.0 Pass Breakdown = 1.4% Time (min) 17

19 Matrix Study for Semivolatiles Decision Flow Diagram Initial QC and Calibration Inject Matrix Set (N=20) Yes Check QC / CCV / ISTD Pass Check? No Corrective Maintenance Attempt 1? No Corrective Maintenance Attempt 2 Yes Yes No Change septum, liner Replace Guard Chip Yes Pass Check? Check QC / CCV / ISTD New Column No Check QC / CCV / ISTD MSD Maintenance 18

20 Semivolatiles Matrix Extracts Composite soil extracts in methylene chloride from ESC Lab Sciences (Mt. Juliet, TN) 20 sequential injections on a wool packed liner Typical of their Worst Matrix 19

21 Tailing Factor at 10% Peak Height Semivolatiles Suitability Standard - Matrix Study Pentachlorophenol and Benzidine Tailing Factor After Liner Change Guard Chip Replacement Pentachlorophenol Benzidine 0 Number of Matrix Injections 20

22 Calibration Check % Error Semivolatiles Calibration Verification Matrix Study Continuing Calibration Verification 40 After Calibration Compound Number 21

23 Calibration Check % Error Semivolatiles Calibration Verification Matrix Study Continuing Calibration Verification 40 After Calibration After 680 Matrix Injections Nitrophenol Pentachlorophenol -40 Hexachlorocyclopentadiene Compound Number 22

24 Calibration Check % Error Semivolatiles Calibration Verification Matrix Study Continuing Calibration Verification After Calibration After 680 Matrix Injections After Column Replacement Benzidine Nitrophenol Pentachlorophenol -40 Hexachlorocyclopentadiene Compound Number 23

25 Normalized Internal Standard Area Semivolatiles ISTDs Matrix Study Internal Standard Response ,4-Dichlorobenzene-d4 Acenaphthene-d10 Chrysene-d12 Naphthalene-d8 Phenanthrene-d10 Perylene-d Number of Matrix Injections 24

26 Semivolatiles Comparison to 7890 Matrix Study Parallel studies carried out on 7890 using three separate columns Column Number Number of Liners and Septa Replaced Number of Inlet Seals Replaced Number of Column Trims (30.5 cm each) Total Number of Matrix Injections Mechanisms of failure: Column 1 and 3 - ISTD response below 50% and greater than 10% CCV failure - ISTD response recovered with source cleaning Column 2 greater than 10% CCV failure - CCV failure recovered after column replacement 25

27 Multiresidue Pesticides in Food - Experimental Seven different matrices were prepared with a QuEChERS method including dspe (honey, rice, olive oil, onion, cucumber, black tea, and orange) 21 pesticides and 6 deuterated PAHs (ISTDs) were used for calibration and recovery assessment Intuvo GC was configured with a SSL inlet, simple MS flow path, and 7000C 8 level calibration curve (1ppb to 1000ppb) was performed followed by a cal check with the 50ppb standard then ~60 additional injections with the 50ppb standard 3-layer sandwich injections used standard, analyte protectants, and matrix After calibration, cal check, and matrix analysis maintenance was performed: Septum replacement Liner replacement Guard chip replacement 26

28 Recovery Average recoveries for 60 matrix injections 160 Average recoveries for 60 injections with the seven extracts are shown. Average recovery was 82% RSD was 6.3% 70% and 120% recovery thresholds are noted Honey Rice Orange Black Tea Olive Oil Onion Cucumber 27

29 Intuvo vs. 7890: A longevity comparison Using a spinach matrix, longevity of the Intuvo GC was compared to a 7890 Splitless injection, 15m HP5-MSUI column, simple flow path configuration (no backflush) Iprodione-Intuvo Pre-spinach After 30 spinac matrix inj h After liner replacement t The 7890 lasted 192 matrix injections before being unrecoverable The 15m column prevented significant column trimming in order to maintain a retention time locked system 3 liner changes and 2 column trims were performed Iprodione-7890 The Intuvo lasted 302 matrix injections before being stopped 4 liner changes and 1 guard chip change was performed Pre-spinach After 30 matrix inj After liner replacement Liner replacements often bring the system back into spec 28

30 Intuvo Guard Chip and No-trim Columns No retention time shifts after maintenance Conventional Column Intuvo Column Shifts beyond retention time window Maintaining retention times 0.5 min 0.5 min Pesticide mix injected before and after 300 mm column trim Pesticide mix injected before and after guard chip change 29

31 Speed of Analysis and Recovery of High Boiling Point Compounds 30

32 Total Petroleum Hydrocarbon Analysis Using Ultra- Fast GC Total Petroleum Hydrocarbon Analysis (TPH) C 10 to C 40 hydrocarbon contamination in the environment (water soil, sediment) GC analysis of extracts with FID Measure TOTAL hydrocarbons, speciation not needed Ultra-Fast Gas Chromatography (UFGC) Run times are typically 3-5 minutes - Rapid column heating (> 100 o C/min over entire oven temp range) - Short columns ( 3-5 m) - High carrier flow rates (> 5 ml/min) Challenges - Retention Time Precision precise column temperature control precise flow control during fast column heating 31

33 Intuvo GC Conditions for UFGC TPH Analysis Intuvo GC Conditions: ALS with 10 ml syringe, 1 ml injection SSL Inlet, splitless mode, UI liner, 400 o C, Merlin Microseal Guard Chip, 350 o C D1 Flow Path, 350 o C DB-1ht column, 5 m x 0.32 mm ID x 0.1 mm - helium carrier 10 ml/min constant flow - oven program, 40 o C for 0.5 min, 250 o C/min to 350 o C, hold 0.5 min FID, 350 o C TPH Samples - Certified Reference Materials from Bundesanstalt für Materialforschung und prüfung (BAM) Soil and river sediment sample matrices Real samples with certified consensus TPH values Samples prepared in LFS using ISO protocol - Ultrasonic liquid/solid extraction, centrifuge fines, liquid/liquid cleanup, SPE final cleanup 32

34 UFGC TPH Discrimination Performance Test pa C10 C12 C14 C16 C18 C20 C22C24 C26 C28 C30 C32 C34 C36 C38 C40 Peak Area Recovery C C C C C C20* C C C C C C C C C C C40 recovery must be > min. 33

35 UFGC TPH Repeatability Test 3 Runs of Mid-Level Calibration Standard pa FID1 A, Front Signal (C:\CHEM32\...TPH_APP_160225\AP73_TPH_APP_ \Check Std 03.D) FID1 A, Front Signal (C:\CHEM32\...TPH_APP_160225\AP73_TPH_APP_ \Check Std 04.D) FID1 A, Front Signal (C:\CHEM32\...TPH_APP_160225\AP73_TPH_APP_ \Check Std 05.D) C 10 C44 Std 3 Run Peak Area Mean Std Dev RSD 1.7% 400 ISO RSD <= 5% 200 TPH Standard min 34

36 UFGC TPH Analysis in Soil Sample Analysis Precision pa pa C10 min pa C min Retention Time (min.) C10 C40 Avg StdDev RSD 0.109% 0.018% min 35

37 UFGC TPH Analysis for Soil and Sediment Samples pa BAM-U021 Certified Reference Sample Mineral Oil Contaminated Soil 2000 C TPH C 44 min pa BAM-U022 Certified Reference Sample Mineral Oil Contaminated Sediment C TPH C 44 min 36

38 TPH Sample Performance Using the Intuvo UFGC U021 A U021 B U022 A U022 B Run mg/kg mg/kg mg/kg mg/kg Mean Cert. Value / /- 550 Std Dev RSD 0.82% 0.15% 0.37% 0.60% r (exp) r* (ref) *r = ISO single lab precision requirement Intuvo Delivers: Method Accuracy Instrument Precision Method Precision 37

39 Ease of Use 38

40 Why Intuvo for D7504 Analysis of Aromatic Solvent Purity D7504 is designed as an easy-to-use method - no sample prep or calibration standards Why not have an easy-to use GC for an easy-to-use method? Intuvo s FID is ideally suited to using Effective Carbon Number response - uses the Agilent s FID with autoranging electronics detector response is linear for peaks from ppm to 99+ % in a single run Intuvo is an easy to use - Intuvo click and run connections for easy column install - Intuvo Smart ID Key - Intuvo Health Report and Early Maintenance Feedback (EMF) 39

41 D7504 Analysis of Purified Toluene Precisely measures very large components (99.97%) and very small components (18 ppm) in a single run pa pa pa toluene non-aromatics 0.16 pa min C9+ aromatics ethylbenzene min 40

42 Summary of Intuvo Applications by Industry Environmental EPA Method 8270 Semivolatile Organic Compounds (SVOC) Ultra-Fast Total Petroleum Hydrocarbons (TPH) Energy and Chemical Ultra-Fast SimDis using ASTM D7798 Monocyclic Aromatic Purity using ASTM D7504 Sulfur in Light Petroleum Liquids Visit Agilent s Website to Download the Application Notes and Briefs! Food Multiresidue Pesticide Analysis in Food Pharma Extractables and Leachables Consumer Products Longevity of Phthalates Fragrance Impurities using Intuvo with OpenLab Match/Compare 41