New Integrated GC Guard Columns for Built-In Protection

Transcription

1 New Integrated GC Guard Columns for Built-In Protection

2 GUARDIAN: Quick and Easy to Set-up With Guardian you get your column plus 5 or 10 meters of additional integrated deactivated tubing. However, it s quick and easy to replace your existing column with a Guardian. GC s with Electronic Pressure Control (EPC) A GC with EPC controls typically asks you to manually enter your new column dimensions so that it may calculate the flow rate. It is necessary to account for the additional 5 or 10 meters of guard column and input accordingly. For instance, if you were using a typical 30-meter column and you added a 10-meter Guardian, you should enter the overall column length as a 40-meter column. Next, inject a standard test mix, evaluate the changes in chromatography, and further optimize your new Guardian by increasing or decreasing head pressure as necessary.* GC s without Electronic Pressure Control (EPC) Using an older system where column head pressure is fixed and flow rate is manually calculated - at a specific temperature - requires a slightly different adjustment. We suggest you increase starting head pressure by about 15% for 5-meter Guardian and 30% for 10-meter Guardian columns. Next, inject a standard test mix, evaluate the changes in chromatography, and further optimize your new Guardian by increasing or decreasing head pressure as necessary.* * Please remember these are intended only as guidelines. We strongly recommend you actually measure linear flow rate by injection of an unretained peak such as methane or by using a flow meter. For more information on how to measure flow rate, please contact your local Phenomenex representative.

3 Advantages of New Guardian TM Technology vs. Traditional Union Connected Guard Column 4Complete deactivation 4Minimal affect on chromatography 4Quick set-up 4Simple installation 4Out-of-box Built-in column protection Zebron s NEW Guardian eliminates traditional guard column problems because it does away with manual installations and the need for capillary unions. vs. Easy to Recognize Guardian integrated guards are easily recognized. Small identification labels are placed at the guard end of every column. Besides labeling, the entire 5-meter or 10-meter guard section is left ungrouped as a small loop of heat resistant string surrounding the rest of the column; you will always be able to instantly identify which end of your column is the Guardian end. Additional 5-10 meter Guard Column Section Analytical Column Section

4 Benefits of Using a Guard Column 4 Extends column lifetime 4 Traps non-volatile contaminants 4 Prevents stationary phase damage 4 Increases focusing for low boiling compounds Guardian extends column lifetime when analyzing samples that are acidic/basic, contain inorganic salts, or contain other impurities. Contaminants are trapped within the first 4-6 inches of the integrated guard section of column, thus allowing easy removal of contaminated guard section during normal maintenance procedures Since the guard section of column does not contain stationary phase, separation is not affected by maintenance trimming. As a result, column lifetime is typically increased by at least 20%. Effect of Guard Column on Chromatography The guard portion of the column doesn t significantly effect separation or retention as is shown in the examples below. Polyaromatic Hydrocarbon (PAH) separations exhibit similar resolution without changes in retention time. Little or No Affect on chromatography APP ID: Polynuclear Aromatic Hydrocarbons (PAHs) Column: Zebron ZB-5 Gold Seal: Phenomenex single Dimensions: 30meter x 0.25mm x 0.25µm groove (AG0-7518) Order No.: 7HG-G Liner: Single taper/gooseneck Injection: Split 260 C, 1µL liner (AG0-4680) Carrier Gas: Constant Flow Helium 1.4mL/min Septum: Phenomenex Oven Program: 170 C to 240 C at 15 C/min to 275 C PhenoRed-400 at 4 C/min to 320 C at 10 C/min for 5 min (AG0-4690) Detector: MSD@240 C Sample 1. Naphthalene 2. Acenaphthene 3. Acenaphthylene 4. Fluorene 5. Phenanthrene 6. Anthracene 7. Fluoranthene 8. Pyrene 9. Benz[a]anthracene 10. Chrysene 11. Benzo[b]fluoranthene 12. Benzo[k]fluoranthene 13. Benzo[a]pyrene 14. Indeno[1,2,3-cd]pyrene 15. Dibenz[a,h]anthracene 16. Benzo[g,h,i]perylene Notes: All analytes are 100µg/mL in methylene chloride. APP ID: Polynuclear Aromatic Hydrocarbons (PAHs) Column: Zebron ZB-5 with 5 meter Guardian Gold Seal: Phenomenex single Dimensions: 30meter x 0.25mm x 0.25µm groove (AG0-7518) Order No.: 7HG-G GGA Liner: Single taper/gooseneck Injection: Split 260 C, 1µL liner (AG0-4680) Carrier Gas: Constant Flow Helium 1.4mL/min Septum: Phenomenex Oven Program: 170 C to 240 C at 15 C/min to 275 C PhenoRed-400 at 4 C/min to 320 C at 10 C/min for 5 min (AG0-4690) Detector: MSD; amu Sample 1. Naphthalene 2. Acenaphthene 3. Acenaphthylene 4. Fluorene 5. Phenanthrene 6. Anthracene 7. Fluoranthene 8. Pyrene 9. Benz[a]anthracene 10. Chrysene 11. Benzo[b]fluoranthene 12. Benzo[k]fluoranthene 13. Benzo[a]pyrene 14. Indeno[1,2,3-cd]pyrene 15. Dibenz[a,h]anthracene 16. Benzo[g,h,i]perylene Notes: All analytes are 100µg/mL in methylene chloride. Should you be using a guard column? If you are working with samples that contain unknown impurities, chances are you should be using a guard. Guard columns help protect your expensive analytical column by: 1 Trapping non-volatiles that would otherwise deposit on the column 2 Prevent acidic and/or basic matrix compounds from stripping the stationary phase 3 Reduces retention time shifts due to column maintenance. Bottom line, if you want longer column life, you should be using a guard column!

5 APP ID: EPA Method 8270C: Semivolatiles Column: Zebron ZB-5 with 5 meter Guardian Dimensions: 30meter x 0.25mm x 0.25µm Order No.: 7HG-G GGA Injection: Split 250 C, 1µL Carrier Gas: Constant Flow Helium 0.9 ml/min Oven Program: 50 C for 2 min to 70 C at 20 C/min to 195 C at 16 C/min to 285 C at 30 C/min to 320 C at 6 C/min for 4min Detector: MSD; amu min , ,70 84,85 103, , , , , , , N-Nitrosodimethylamine 2. Pyridine 3. 2-Picoline 4. N-Nitrosomethylethylamine 5. Methyl methanesulfonate 6. N-Nitrosodiethylamine 7. Ethyl Methanesulfonate 8. Phenol 9. Aniline 10. Bis(2-chloroethyl)ether Chlorophenol 12. 1,3-Dichlorobenzene 13. 1,4-Dichlorobenzene 14. Benzyl Alcohol 15. 1,2-Dichlorobenzene Methylphenol 17. Bis(chloroisopropyl)ether Methylphenol; 3-Methylphenol 19. N-Nitrosopyrrolidine 20. Acetophenone 21. N-Nitrosomorpholine 22. N-Nitroso-di-n-propylamine Toluidine 24. Hexachloroethane 25. Nitrobenzene 26. N-Nitrosopiperidine 27. Isophorone Nitrophenol 29. 2,4-Dimethylphenol 30. Benzoic Acid 31. Bis(2-chloroethoxy)methane 32. 2,4-dichlorophenol 33. µ,µ-dimethylphenylamine 34. 1,2,4-Trichlorobenzene 35. Naphthalene Chloroaniline 37. 2,6-Dichlorophenol 38. Hexachloropropene 39. Hexachloro-1,3-Butadiene 40. 1,4-Phenylenediamine 41. N-Nitrosodibutylamine Chloro-3-methylphenol 43. Safrole Methylnaphthalene Methylnaphthalene 46. 1,2,4,5-Tetrachlorobenzene 47. Hexachlorocyclopentadiene 48. 2,4,6-Trichlorophenol 49. 2,4,5-Trichlorophenol 50. Isosafrole (isomer) Chloronaphthalene Nitroaniline 53. 1,4-Naphthoquinone 54. Dimethyl phthalate 55. 1,3-Dinitrobenzene 56. 2,6-Dinitrotoluene 57. Acenaphthylene Nitroaniline 59. Acenaphthene 60. 2,4-Dinitrophenol Nitrophenol 62. Dibenzofuran 63. Pentachlorobenzene 64. 2,4-Dinitrotoluene Naphthalenamine Naphthalenamine 67. 2,3,4,6-Tetrachlorophenol 68. Diethyl Phthalate 69. Fluorene Chlorophenyl phenyl ether Methyl-5-nitroaniline Nitroaniline Methyl-4,6-dinitrophenol 74. Diphenlamine 75. Azobenzene 76. 1,3,5-Trinitrobenzene 77. Phenacetin Bromophenyl phenyl ether 79. Hexachlorobenzene Aminobiphenyl 81. Pentachlorophenol 82. Pentachloronitrobenzene 83. Phenanthrene 84. Dinoseb 85. Anthracene 86. Di-n-butylphthalate Nitroquinoline-n-oxide 88. Methapyrilene 89. Isodrin 90. Fluoranthene 91. Benzidine 92. Pyrene 93. Aramite (isomer) 94. Aramite (isomer) 95. Dimethylaminoazobenzene 96. Chlorobenzilate 97. 3,3-Dimethylbenzidine 98. Benzyl butyl phthalate 99. Kepone Acetylaminofluorene ,3 -Dichlorobenzidine 102. Benz[a]anthracene 103. Bis(2-ethylhexyl)phthalate 104. Chrysene 105. Di-n-octyl phthalate 106. Benzo[b]fluoranthene 107. Benzo[k]fluoranthene 108. Benzo[a]pyrene Methylcholanthrene 110. Indeno[1,2,3-cd]pyrene 111. Dibenz[a,h]anthracene 112. Benzo[g,h,i]perylene

6 PRINTED MATTER: If Zebron does not provide you with equivalent separations as compared to any other GC column of the same phase and comparable dimensions, send in your comparative data within 45 days and keep the Zebron column for FREE! Ordering Information GC Column Phase Dimensions 5m Guardian TM Order No. Price 10m Guardian TM Order No. Price ZB-1ms Zebron ZB-1ms 30 meter x 0.25 mm x 0.25 df(μm) 7HG-G GGA 7HG-G GGC ZB-5ms Zebron ZB-5ms 15 meter x 0.25 mm x 0.25 df(μm) 7EG-G GGC Zebron ZB-5ms 30 meter x 0.25 mm x 0.25 df(μm) 7HG-G GGA 7HG-G GGC Zebron ZB-5ms 30 meter x 0.25 mm x 0.50 df(μm) 7HG-G GGA Zebron ZB-5ms 30 meter x 0.25 mm x 1.00 df(μm) 7HG-G GGC Zebron ZB-5ms 30 meter x 0.32 mm x 1.00 df(μm) 7HM-G GGA ZB-5 Zebron ZB-5 30 meter x 0.25 mm x 0.25 df(μm) 7HG-G GGA 7HG-G GGC Zebron ZB-5 30 meter x 0.25 mm x 0.50 df(μm) 7HG-G GGA 7HG-G GGC Zebron ZB-5 60 meter x 0.25 mm x 0.25 df(μm) 7KG-G GGA Zebron ZB-5 60 meter x 0.25 mm x 0.50 df(μm) 7KG-G GGA 3320_I