Productivity Improvements for Dual Column Applications: USP <467> and Blood Alcohol Analysis

Transcription

1 Productivity Improvements for Dual Column Applications: USP <467> and Blood Alcohol Analysis Rebecca Veeneman, PhD Applications Chemist Agilent Technologies

2 The GC Usability Gap Usability lags features and performance Over the years, GC features and performance have matured and largely serve today s needs Usability has lagged and does not meet today s expectations, especially as expertise more scarce Time Features and performance Usability

3 A Time for Change Shifting Demographics Seasoned operators retiring; highly skilled replacements harder to find GC troubleshooting skills not necessarily located at point of use Operators assuming multiple responsibilities Challenging Economics Budgets squeezed, expectations increased Capital purchase decisions made on business NOT technical basis

4 The GC Community Voice We listened and responded Improve the User Experience Install and setup Use and maintain Make GC more practical for today s busy lab enterprise Prepare for Next Generation of Users Its not only about better analytical performance its about better lab outcomes

5 Make GC Easier

6 Innovating a New Path to GC Productivity A whole new way to GC Easier Faster Smaller Smarter Greener

7 Innovating a New Path to GC Productivity A whole new way to GC Direct heating

8 Innovating a New Path to GC Productivity A whole new way to GC No-trim column Direct heating

9 Innovating a New Path to GC Productivity A whole new way to GC Ferrule-free click-andrun connections No-trim column Direct heating

10 Innovating a New Path to GC Productivity A whole new way to GC Disposable Guard chip Ferrule-free click-andrun connections No-trim column Direct heating

11 Innovating a New Path to GC Productivity A whole new way to GC Modular Intuvo flow chips Disposable Guard chip Ferrule-free click-andrun connections No-trim column Direct heating

12 Flexible Compatible Design Configurable to any application SSL, MMI, GSV, LSV inlets FID, TCD, ECD, NPD, FPD, NCD, SCD detectors SQ and TQ mass spectrometers Headspace sampler, CTC PAL 16-, 50-, 150-position auto-injectors and trays Software: OpenLAB and MassHunter

13 Innovating the GC Flow Path Conventional flow path Inlet Gold seal Nuts and ferrules Classic capillary column To detector

14 Innovating the GC Flow Path Conventional flow path Intuvo flow path Inlet Inlet Guard chip Gold seal Nuts and ferrules Classic capillary column To detector

15 Innovating the GC Flow Path Conventional flow path Intuvo flow path Inlet Inlet Click-andrun direct connections Guard chip Gold seal Nuts and ferrules To detector Flow chip Classic capillary column Intuvo planar column To detector

16 Click-and-Run Direct Connections Eliminating connection uncertainty No more ferrules Direct face seal connections Audible and tactile click lets you know connection is made Easier to train Less unplanned downtime Fewer batch reruns and precious samples lost

17 Autonomous Leak Checking Avoiding unplanned downtime Microfluidic-enabled 6 th generation EPC modules allow hands-free leak checking to confirm and document leak-free operation autonomously.

18 Intuvo Graphical User Interface System status

19 Intuvo Graphical User Interface System status Real-time chromatograms pa min.

20 Intuvo Graphical User Interface System status Real-time chromatograms Step-by-step user maintenance and troubleshooting

21 Intuvo Graphical User Interface System status Real-time chromatograms Find Inlet Parts Replace Inlet Liner Step-by-step user maintenance and troubleshooting Inlet Liner Part Number Finding parts fast

22 Internet of Things A full suite of customer support Intuvo serves up a web homepage User help for Maintenance, troubleshooting and diagnostics Accessible through any approved PC or mobile device securely on internal network

23 Agilent CrossLab Services for Intuvo A streamlined service experience Intuvo 9000 GC Health Report Installation and familiarization Compliance services Education

24 A simplified approach to dual column analyses USP <467> and Blood Alcohol Analysis

25 Dual Column Configuration Headspace to SSL Inlet splitter flow chip equally splits to two columns Two columns to two FID detectors Inlet FID 1 FID 2 Guard Chip Inlet splitter flow chip Column 2 Column 1

26 USP <467> Residual Solvent Requirements Analysis of residual solvent is a critical application in the pharmaceutical industry. The choice of solvent during manufacturing can improve yield or affect chemical properties of the product synthesized. Solvents do not enhance the product s efficacy and must be removed as completely as possible to meet product specification and good manufacturing practices. USP467 specifies a single column analysis A secondary analysis is performed if the solvent is found above limit detection. With an Intuvo 9000 GC configured with an inlet splitter both analyses can be performed in one analytical run. USP 40, general chapter USP <467> Residual Solvents

27 USP <467> Residual Solvent Methods Class 1, Class 2A, and Class 2B residual solvents were prepared at their limit concentrations in water. Class 1 ( ) was prepared in the following manner: ml stock and 9 ml of DMSO was diluted to 100 ml with water ml from step 1 was diluted to 100 ml with water ml from step 2 was diluted to 100 ml with water Class 2A ( ) was prepared by diluting 1 ml of stock to 100 ml with water Class 2B ( ) was prepared by diluting 1 ml of stock to 100 ml with water Headspace vials were prepared with 1 ml of each solvent and 5 ml of water. Samples were prepared in 10 ml HS vials

28 7697 Headspace parameters 7697 Headspace Sampler Setpoint Oven 85 C Loop 85 C Transfer line 100 C Vial equilibration time Injection duration Vial size Vial shaking Vial fill mode Vial fill pressure Loop ramp rate Loop final pressure Loop equilibration time 40 min 0.5 min 10 ml Level 2, 25 shakes/min Default (50 ml/min to 15 psi (0.1 min)) 15 psi 20 psi/min 0 psi 0.05 min

29 Intuvo Parameters Intuvo 9000 GC Oven Setpoint 40 C (5 min) 15 C/min to 180 C (3 min) Split/Splitless Inlet Split 5:1, 140 C DB-624 Select UI ( UI-INT) 30m x 320µm x 1.8µm DB-WAX UI ( UI-INT) 30m x 320µm x 0.25µm Constant flow 2 ml/min Controlled by column 1 FID (Front and Back) 250 C H2 Air 30 ml/min 400 ml/min N2 (makeup) 25 ml/min Jumper chip 140 C Bus 200 C Front/Back Signal 20 Hz Jumper chip set to the inlet temperature Bus temperature set to default

30 Response [pa] Class 1, DB-624 Select UI x10 1 DFID_Mix1_01 FID3A 1. 1,1-dichloroethane 2. 1,1,1-trichloroethane 3. carbon tetrachloride 4. benzene 5. 1,2-dichloroethane Retention time [min]

31 Response [pa] Class 1, DB-WaxUI 1. 1,1-dichloroethane 2. 1,1,1-trichloroethane 3. carbon tetrachloride 4. benzene 5. 1,2-dichloroethane x DFID_Mix1_01 FID4B 1 2, Retention time [min]

32 Class 1 Repeatability Compound DB-624 RT RSD DB-624 Area RSD DB-Wax RT RSD DB-Wax Area RSD 1,1-dichloroethane 0.031% 3.3% 0.074% 2.1% 1,1,1- trichloroethane carbon tetrachloride 0.026% 2.6% 0.037% 1.8% 0.11% 4.8% Coelutes with 1,1,1- trichloroethane Coelutes with 1,1,1- trichloroethane benzene 0.017% 2.2% 0.055% 1.5% 1,2-dichloroethane 0.016% 3.9% 0.067% 1.8% Excellent RT repeatability for both columns Area RSD is 5% or better

33 Response [pa] Class 2A, DB-624 Select UI 1. methanol 2. acetonitrile 3. methylene chloride 4. trans-1,2-dichloroethene 5. cis-1,2-dichloroethene 6. tetrahydrofuran 7. cyclohexane 8. methylcyclohexane 9. 1,4-dioxane 10.toluene 11.chlorobenzene 12.ethylbenzene 13.m-xylene 14.p-xylene 15.o-xylene x DFID_Mix2a_01 FID3A , Retention time [min]

34 Response [pa] Class 2A, DB-Wax UI 1. methanol 2. acetonitrile 3. methylene chloride 4. trans-1,2-dichloroethene 5. cis-1,2-dichloroethene 6. tetrahydrofuran 7. cyclohexane 8. methylcyclohexane 9. 1,4-dioxane 10.toluene 11.chlorobenzene 12.ethylbenzene 13.m-xylene 14.p-xylene 15.o-xylene x DFID_Mix2a_01 FID4B , Retention time [min] 11

35 Class 2A Repeatability Compound DB-624 RT RSD DB-624 Area RSD DB-Wax RT RSD DB-Wax Area RSD methanol acetonitrile methylene chloride 0.23% 1.3% 0.22% 1.3% 0.023% 2.1% 0.039% 2.0% 0.018% 0.98% 0.033% 0.78% trans-1,2- dichloroethene 0.016% 0.85% 0.023% 0.72% cis-1,2- dichloroethene 0.012% 0.76% 0.039% 0.78% tetrahydrofuran cyclohexane methylcyclohexane 1,4-dioxane toluene chlorobenzene ethylbenzene m-xylene p-xylene o-xylene coleutes with 0.018% 0.95% methylene chloride coelutes with methylene chloride 0.011% 0.96% 0.013% 0.96% % 0.98% 0.018% 0.96% 0.012% 1.9% 0.025% 1.7% % 0.81% 0.029% 0.80% % 0.69% 0.024% 0.69% % 0.84% 0.016% 0.86% % 0.82% 0.017% 0.82% coleutes with m-xylene coelutes with m-xylene 0.015% 0.83% % 0.81% 0.026% 0.81% Excellent RT repeatability for both columns Area RSD is 2% or better

36 * peak@7.815min * peak@8.476min peak@10.642min peak@6.230min peak@9.247min peak@11.334min Response [pa] peak@16.463min Class 2B, DB-624 Select UI 1. n-hexane 2. nitromethane 3. chloroform 4. 1,2-dimethoxyethane 5. trichloroethane 6. pyridine 7. 2-hexanone 8. tetralin x DFID_Mix2b_01 FID3A Retention time [min]

37 Response [pa] Class 2B, DB-Wax UI 1. n-hexane 2. nitromethane 3. chloroform 4. 1,2-dimethoxyethane 5. trichloroethane 6. pyridine 7. 2-hexanone 8. tetralin x DFID_Mix2b_01 FID4B Retention time [min] 6 8

38 Class 2B Repeatability Compound DB-624 RT RSD DB-624 Area RSD DB-Wax RT RSD DB-Wax Area RSD hexane 0.021% 4.4% 0.039% 4.5% nitromethane 0.42% 4.9% 0.018% 2.5% chloroform % 4.8% 0.026% 3.2% 1,2- dimethoxyethane 0.013% 3.6% 0.089% 3.6% trichloroethane 0.010% 3.4% 0.028% 3.1% pyridine 0.020% 1.7% 0.034% 1.7% 2-hexanone % 2.7% 0.020% 0.74% tetralin % 2.1% 0.016% 2.1% Excellent RT repeatability for both columns Area RSD is 5% or better

39 Blood Alcohol Concentration Application Requirements Determination of blood alcohol concentration requires rigorous control. Many labs use flame ionization detection (FID) which lacks identification capabilities Often a second system with a column having different retentive properties is used to confirm analyte identification Blood alcohol concentration determination and confirmation can be achieved simultaneously with the Intuvo 9000 GC. An Intuvo 9000 GC system was equipped with a 7697A Headspace Sampler and configured with an inlet splitter allowing dual column, dual FID analysis. NEW BAC UI columns ( UI-INT and UI- INT) were used which improve resolution of critical analytes

40 7697 Headspace parameters 7697 Headspace Sampler Set point Oven 70 C Loop 70 C Transfer line 90 C Vial equilibration time Injection duration Vial size Vial shaking Vial fill mode Vial fill pressure Loop ramp rate Loop final pressure Loop equilibration time 7 min 0.5 min 20 ml Off Default (50 ml/min to 15 psi (0.1 min)) 15 psi 30 psi/min 1.5 psi 0.05 min

41 Intuvo Parameters Intuvo 9000 GC Oven Set point 40 C (6.5 min) Split/Splitless Inlet Split 10:1, 110 C DB-BAC1 UI ( UI-INT) 30m x 320µm x 1.8µm DB-BAC2 UI ( UI-INT) 30m x 320µm x 1.2µm Constant pressure 21 psi Controlled by column 1 FID (Front and Back) 250 C H2 Air 30 ml/min 400 ml/min N2 (makeup) 25 ml/min Jumper chip 110 C Bus Default (On 200 C) Front/Back Signal 20 Hz Jumper chip set to the inlet temperature Bus temperature set to default

42 Response Response Calibration Results Calibration standards were made in house 0.8% to 0.01% methanol, acetaldehyde (not quantified), acetone, ethanol, and isopropanol. Headspace vials were made in triplicate at each level consisting of 450 µl of internal standard (0.3% n- propanol) and 50µL of standard. Calibration curves for methanol, ethanol, acetone, and isopropanol are shown right. Ethanol calibration curves yield or better for the dual column ensemble. Slope difference between the two column/detector pairs is 6.3% BAC UI 1 y = x R² = y = x R² = y = x R² = Concentraion (mg/dl) BAC UI 2 y = x R² = y = x R² = y = x R² = Concentraion (mg/dl) y = 0.616x R² = Isopropanol Acetone Ethanol Methanol y = x R² = Isopropanol Acetone Ethanol Methanol

43 Calibration Accuracy Verification Ethanol Standard Calculated Concentration DB-BAC1 UI Pass/Fail Calculated Concentration DB-BAC2 UI Pass/Fail Agilent Ethanol Standards were evaluated on the Intuvo 9000 GC. The concentration of the ethanol standards was calculated based on the calibration curves and compared to the expected concentration. Pass or fail was determined with ± 6% error tolerance. 20 mg/dl ( ) 50 mg/dl ( ) 80 mg/dl ( ) 100 mg/dl ( ) 150 mg/dl ( ) 200 mg/dl ( ) 300 mg/dl ( ) 400 mg/dl ( ) 19.8 mg/dl Pass 19.3 mg/dl Pass 50.0 mg/dl Pass 47.1 mg/dl Pass 79.3 mg/dl Pass 76.8 mg/dl Pass 96.7 mg/dl Pass 94.4 mg/dl Pass 152 mg/dl Pass 149 mg/dl Pass 197 mg/dl Pass 193 mg/dl Pass 302 mg/dl Pass 302 mg/dl Pass 384 mg/dl Pass 386 mg/dl Pass

44 Area Repeatability Area repeatability for the 80mg/dL standard as well as the Agilent Blood Alcohol Checkout mix ( ) was determined for five replicate headspace vials. Analyte DB-BAC1 UI DB-BAC2 UI Ethanol 80 mg/dl standard 3.70% 2.80% Methanol 4.10% 1.40% Acetaldehyde 2.80% 3.00% Ethanol 2.30% 1.10% Isopropanol 3.30% 1.90% t-butanol 2.80% 2.70% Propanal 3.40% 3.00% 4.1% or better! n-propanol 3.10% 2.10% Acetone 3.40% 2.90% Acetonitrile 2.30% 2.80% 2-Butanol 2.00% 3.00% Ethyl acetate 3.20% 3.10% 2-Butanone 3.10% 3.00%

45 Retention Time Repeatability Retention time repeatability for the 80mg/dL standard and the Agilent Blood Alcohol Checkout mix ( ) was determined for five replicate headspace vials. Analyte DB-BAC1 UI DB-BAC2 UI Ethanol 80 mg/dl standard 0.04% 0.10% Methanol 0.01% 0.02% Acetaldehyde 0.01% 0.02% Ethanol 0.02% 0.05% Isopropanol 0.02% 0.04% t-butanol 0.03% 0.04% Propanal 0.01% 0.02% 0.1% or better! n-propanol 0.03% 0.04% Acetone 0.02% 0.03% Acetonitrile 0.02% 0.03% 2-Butanol 0.04% 0.04% Ethyl acetate 0.02% 0.03% 2-Butanone 0.02% 0.03%

46 DB-BAC1 UI Blood Alcohol Checkout Mix New columns resolve t-butanol and n-propanol from analytes of interest 2 Average resolution = Methanol 2. Acetaldehyde 3. Ethanol 4. Isopropanol 5. t-butanol 6. Propanal 7. n-propanol 8. Acetone 9. Acetonitrile 10.2-Butanol 11.Ethyl Acetate 12.2-Butanone

47 DB-BAC2 UI Blood Alcohol Checkout Mix Elution order changes allow confirmation of analyte identification Methanol 2. Acetaldehyde 3. Ethanol 4. Isopropanol 5. t-butanol 6. Propanal 7. n-propanol 8. Acetone 9. Acetonitrile 10.2-Butanol 11.Ethyl Acetate 12.2-Butanone

48 Conclusions Dual column analyses can be easily accomplished with Intuvo Analysis of USP <467> and blood alcohol can be easily accomplished with Intuvo Inlet splitting capability enables analysis and confirmation in a single run on two columns of different phases Can be configured as an Agilent Analyzer as well Includes a factory method and report template New columns (DB-624 Select UI, DB-Wax UI, DB-BAC 1 UI and DB-BAC 2 UI) yield excellent resolution of the analytes of interest in their application space. Excellent linearity, retention time repeatability, and area repeatability were achieved with both applications configured with the inlet splitter.

49 Thank you for your attention Any questions?