Think GC/MS Couldn t Get Any Better? Think Again!
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1 Think GC/MS Couldn t Get Any Better? Think Again! Maggie Ostrowski, PhD & Rebecca Veeneman, PhD Think GC/MS Couldn t Get Any Better? Think Again! June 7, 2017
2 Introducing Intuvo 9000 GC System A completely re-imagined integrated system of GC consumables, MS services and software New paradigm streamlining the way GC is done Better Business Outcome
3 An Ideal Partner for Mass Spectrometry Guard chip protection of column and MS ion source Retention time stability after changing guard chip Easy and reliable backflush Leak-free click-and-run connections Fast direct heating and cooling
4 Innovating a New Path to GC Productivity A whole new way to GC Direct heating
5 Innovating a New Path to GC Productivity A whole new way to GC No-trim column Direct heating
6 Innovating a New Path to GC Productivity A whole new way to GC Ferrule-free click-and-run connections No-trim column Direct heating
7 Innovating a New Path to GC Productivity A whole new way to GC Disposable Guard chip Ferrule-free click-and-run connections No-trim column Direct heating
8 Innovating a New Path to GC Productivity A whole new way to GC Modular Intuvo flow chips Disposable Guard chip Ferrule-free click-and-run connections No-trim column Direct heating
9 Innovating the GC Flow Path Conventional flow path Intuvo flow path Inlet Inlet Click-and-run direct connections Guard chip Gold seal Nuts and ferrules To detector Flow chip Classic capillary column Intuvo planar column To detector
10 Click-and-Run Direct Connections Eliminating connection uncertainty No more ferrules Direct face seal connections Audible and tactile click lets you know connection is made Easier to train Less unplanned downtime Fewer batch reruns and precious samples lost
11 Autonomous Leak Checking Avoiding unplanned downtime Microfluidic-enabled 6 th generation EPC modules allow hands-free leak checking to confirm and document leak-free operation autonomously.
12 Fast Direct Heating and Cooling Every single minute counts Fast, efficient heating Ballistic cooling Smaller footprint
13 Chromatographic Comparison: Which is Intuvo? 8270 Calibration Standard 77 Acids/Bases/Neutrals (PAHs), 6 ISTDs??
14 Chromatographic Comparison: Which is Intuvo? 8270 Calibration Standard 77 Acids/Bases/Neutrals (PAHs), 6 ISTDs Intuvo 7890
15 Column Trims Can Substantially Impact Retention Times 1 22, , Blue trace: original chromatogram Red trace: after trimming 300mm , , ,4-dichlorobenzene-d4 2. Naphthalene-d8 3. Methacrifos 4. Acenaphthene-d10 5. Ethalfluralin 6. Sulfotep 7. Demeton-S 8. Simazine 9. Lindane 10. Phenthrene-d Chlorpyrifos methyl 12. Fenitrothion 13. Aldrin 14. Pendimethalin 15. Tolyfluanid 16. Dieldrin 17. Bupirimate 18. Triazophos 19. Chrysene-d Iprodione 21. EPN 22. Phosalone 23. Mirex 24. Coumaphos 25. Perylene-d Pryaclostrobin 27. Deltamethrin June 14,
16 Guard Chip Changes Do Not Affect Retention Times 1 Red trace: original chromatogram Blue trace: after guard chip replacement 22, , , ,4-dichlorobenzene-d4 2. Naphthalene-d8 3. Methacrifos 4. Acenaphthene-d10 5. Ethalfluralin 6. Sulfotep 7. Demeton-S 8. Simazine 9. Lindane 10. Phenthrene-d Chlorpyrifos methyl 12. Fenitrothion 13. Aldrin 14. Pendimethalin 15. Tolyfluanid 16. Dieldrin 17. Bupirimate 18. Triazophos 19. Chrysene-d Iprodione 21. EPN 22. Phosalone 23. Mirex 24. Coumaphos 25. Perylene-d Pryaclostrobin 27. Deltamethrin
17 lidocaine bis(2-hydroxyethyl) terephthalate methylparaben polyparaben methylparaben polyparaben lidocaine bis(2-hydroxyethyl) terephthalate Complex Samples Can Be Easily Evaluated Film Lidocaine patch A 5% lidocaine transdermal patch and the film was extracted in dichloromethane Paraben A, paraben B, lidocaine (active ingredient), and a terephthalate was found in both samples via extracted ion chromatograms
18
19 GC-MS Systems Are Hindered by Maintenance 1. Vent MS (wait for system to cool and reach acceptable vacuum levels) 2. Replace liner and trim column 3. Pump down MS 4. Air and water check 5. Verify chromatogram 6. Re-lock retention times 7. Return to running samples
20 Trimming The Column Results In Retention Time Shifts June 14,
21 I m going to be late Oh no! Why? Maintenance on the GC-MS Ok. See you when you get here. I ll let you know when I m on my way
22 I m going to be late Oh no! Why? Maintenance on the GC-MS Ok. See you when you get here. I ll let you know when I m on my way
23
24 Intuvo Systems Simplify Maintenance 1. Press maintenance button 2. Replace liner and guard chip 3. Air and water check 4. Verify chromatogram 5. Return to running samples
25 Replacing the Guard Chip Does Not Affect Retention Times
26 What about the MSD? JetClean Self-cleaning ion source Patented Consistent response for months or even years Reduces or eliminates the downtime for manual source cleaning Available on single and triple quad GC/MS systems, including Intuvo
27 JetClean Scrubs Away Contamination Contamination example After JetClean JetClean eliminates heavy contamination from the lens
28 JetClean for PAH Analysis 62 PAHs, archetypally difficult Range 1-10,000pg/μl, R 2 >0.998 Non-reactive, nonpolar compound classes are generating exceptional results in the Acquire & Clean operational mode 5% of true values, over many months Mean limits of LODs / pg/μl
29 Manual Source Cleaning Frequency Is Significantly Reduced Standard system August September October November 2014 December January February March JetClean system August September October November 2014 December January February March April May June July April May June July August September October November 2015 December January February March August September October November 2015 December January February March April May June April May June Monthly No Cleaning in 23 months!
30 Finishing up on my way You re early Yep Intuvo Ok, see you soon!
31 Finishing up on my way You re early Yep Intuvo Ok, see you soon!
32 Finishing up on my way You re early Yep Intuvo Ok, see you soon!
33 Difficulty Intuvo/MSD with JetClean Can Save Time and Money Conventional GC/MS maintenance Intuvo/JetClean maintenance Time
34 Thank You! Questions?
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