Same Column and Gas Type Try Different/Faster Velocities. Same Column, Switch He to H2 Carrier Then Try Faster Velocities

Transcription

1 : What Are You Willing To Do? Daron Decker Chromatography Technical Specialist Page 1 Agilent Restricted 3 Options Same Column and Gas Type Try Different/Faster Velocities Same Column, Switch He to H2 Carrier Then Try Faster Velocities Different Column Dimensions(H2 Carrier) Then Try Faster Velocities Page 2 1

2 Considerations If I want to try -Higher carrier gas linear velocity -Utilizing Hydrogen as carrier gas -Smaller I.D./shorter length columns I ll need to -Properly adjust the temperature programs Method Translation Software to the Rescue Page 3 Method Translation Software Input Screen Page 4 2

3 CLP Standard Compound List 1. TCMX 12. 4,4 DDE 2. Alpha BHC 13. Dieldrin 3. Gamma BHC 14. Endrin 4. Beta BHC 15. 4,4 DDD 5. Delta BHC 16. Endosulfan II 6. Heptachlor 17. 4,4 DDT 7. Aldrin 18. Endrin Aldehyde 8. Heptachlor Epoxide 19. Endosulfan Sulfate 9. Gamma Chlordane 20. Methoxychlor 10. Alpha Chlordane 21. Endrin Ketone 11. Endosulfan I22. DCB Page 5 Original Improved Method 0.32mm I.D., Helium Carrier Gas 1 2 GC D\ECD2B 3 4 5, Column: DB-XLB 30m x 0.32mm i.d., 0.25µm Carrier: He, constant flow, 38 cm/s at 120 C Injector: Pulsed Splittless, 220 C Pulse pressure & time: 35psi for 1.15min 2µL, 50ppb Oven: 120 C for 1.17min 120 C to 160 C at 25 /min 160 C to 260 C at 10 /min 260 C to 300 C (4min) at 15 /min Detector: µ-ecd, 320 C Ar/CH4 (P5) makeup gas at 60mL/min <16 minutes Page 6 3

4 Input Original Method Parameters Page 7 Same Column and Gas Type Best Efficiency Transfer same dimensions New Velocity New Temp. Program Page 8 4

5 Same Column and Gas Type Best Efficiency Still Co-eluting Better Resolution Page 9 Same Column and Gas Type Best Efficiency Better Resolution NEW Original 23 minutes! Page 10 5

6 Same Column and Gas Type Fast Analysis New Velocity New Temp. Program Page 11 Same Column and Gas Type Fast Analysis Co-elution Slight loss of Resolution <14 minutes Page 12 6

7 Same Column and Gas type None mode, Try higher velocities New calculated temp. program Page 13 Same Column and Gas type None mode, Try higher velocities Co-elution 80 cm/sec Co-elution <8 minutes 65 cm/sec Close elution <10 minutes 50 cm/sec Page 14 7

8 van Deemter Curves 1.00 Excessive Diffusion µ opt.. He Poor Mass Transfer H 2 *µ minimum µ (cm/sec) Page 15 Same Column, Hydrogen Carrier Gas Translate Only Close elution 11 minutes Page 16 8

9 Same Column, H2 Carrier Gas Fast Analysis Close elution <10 minutes Page 17 Same Column, H2 Carrier Gas, Higher Velocities Page 18 9

10 Same Column, H2 Carrier Gas, Higher Velocities Co-elution 85 cm/sec Co-elution 7 minutes 80 cm/sec Co-elution <8 minutes 70 cm/sec Page 19 New Column Dimensions, H2 Gas, Translate Only Input NEW dimensions Close enough (got lucky) New Velocity New Temp. Program Page 20 10

11 New Column Dimensions, H2 Gas, Translate Only Page 21 New Column Dimensions, H2 Gas, Translate Only Page 22 11

12 New Column Dimensions, H2 Gas, Fast Analysis New Velocity New Temp. Program Page 23 New Column Dimensions, H2 Gas, Fast Analysis Page 24 12

13 New Column Dimensions, H2 Gas, Higher Velocities Page 25 New Column Dimensions, H2 Gas, Higher Velocities 105 cm/sec? 5.5 minutes! 95 cm/sec <6 minutes 85 cm/sec Page 26 13

14 Flow Ramp (EPC) for Late Eluters and Faster Bake Outs Page 27 Flow Ramp No Flow Ramp Begin of Flow Ramp Under 5 minutes! Page 28 14

15 Final Method Used at EPA Column: DB-XLB 20m x 0.18mm i.d., 0.18µm Carrier: H2, constant flow, 77.3cm/s at 120 C Injector: Pulsed Splittless, 220 C Pulse pressure & time: 35psi for 0.5min Flow ramp at 6.25min of 99mL/min 2 to 3mL/min 2mm i.d. liner 0.5µL, 50ppb Oven: 120 C for 0.49min 120 C to 160 C at 59.4 /min C to 260 C at 23.7 /min C to 300 C (1.69min) at 35.6 /min 9 Detector: µ-ecd, 320 C 13 Ar/CH4 (P5) makeup gas at 60mL/min Page 29 Final Method Used at EPA DB-XLB DB-17ms Page 30 15

16 Flow Ramp (EPC) for Late Eluters and Faster Bake Outs Original Method with Helium Page 31 Original Method with Helium GC D\ECD2B No Flow Ramp With Flow Ramp Page 32 16

17 Other Applications PCBs PAHs Steamed Cracked Naphtha Solvents in Drugs Drugs of Abuse Page PCB Congeners High MSD Pumping Capacity Needed 1X 4X Page 34 17

18 39 PCB Congeners HP5-MS, 30 m 1X HP5-MS, 15 m 4X Page PCB Congeners Overlay of 1X and 4X analyses 1X 4X Same chromatograms as on the previous slide Page 36 18

19 PAH s Page 37 PAH s 2.2e+07 2e e e e e+07 1e X 1X Page 38 19

20 PAH s HP5-MS, 30 m HP5-MS, 15 m e e e e X X 40 min 10 min Page 39 More PAH s Page 40 20

21 TIC: 12-04SCN.D More PAH s 16 PAH standards 20 ug/ml - BEFORE PAH 20 ug/ml - AFTER Page 41 TIC: 12-04SCN.D More PAH s 16 PAH standards 20 ug/ml - BEFORE PAH 20 ug/ml - AFTER Page 42 21

22 Steam Cracked Naphtha Page 43 Steam Cracked Naphtha DB-1, 60m x 0.32mm x 0.25um Helium Fast Analysis/Hydrogen Translate to Hydrogen Page 44 22

23 Solvents in Drugs Column: RTX 1701 ; 30 m x 0.32 mm; 1.0 µm film Injection: 1 ml split injection, 25:1, temperature 200 o C, Liner: deactivated quartz tube with glass wool Flow: 10 psi, constant flow Temperature program: 30 o C (7min) 7 o C/min to 80 o C, 15 o C/min to 180 o C (2min) Detection: FID 40 ml/min / air@450 ml/min) Temperature: 240 o C , , Methanol 2. Pentane 3. Ethanol 4. Diethylether 5. Acetone 6. Isopropanol 7. Acetonitrile 8. Methylenechloride 9. t-butanol 10. Methyltertbutylether 11. Hexane 12. n-propylalcohol 13. Ethylacetate 14. Tetrahydrofuran 15. Cyclohexane Isobutylalcohol 17. Isopropylacetate 18. Heptane 19. n-butanol 20. Methylcyclohexane 21. 1,4-Dioxane 22. Methylisobutylketone 23. Toluene 24. Isobutylacetate 25. Ethyleneglycol 26. n-butylacetate 27. Dimethylformamide 28a,b,c. Xylenes 29a,b,c. Dimethylacetamide 30. N-Methylpyrilidone Page Helium as Carrier Gas DB m x 0.25mm x 1.4µm Injector Temperature 225 C Pressure 16.2 psi Split Ratio 25 Split Flow 37 Total Flow 42.2 Flow 1.5 ml/min Mode Constant Flow Oven C/min Temp. (C) Time (min.) Detector FID Hydrogen Flow40 ml/min Air Flow 400 ml/min Mkup 20 ml/min Solvents in Drugs a 28b 28c 29a 1. Methanol 29b 2. Pentane 3. Ethanol 4. Diethylether 5. Acetone 6. Isopropanol 7. Acetonitrile 8. Methylenechloride 9. t-butanol 10. Methyltertbutylether 11. Hexane 12. n-propylalcohol 13. Ethylacetate 14. Tetrahydrofuran Cyclohexane 16. Isobutylalcohol 17. Isopropylacetate 18. Heptane 19. n-butanol 20. Methylcyclohexane 21. 1,4-Dioxane 22. Methylisobutylketone 23. Toluene 24. Isobutylacetate 25. Ethyleneglycol 26. n-butylacetate 27. Dimethylformamide 29C28a,b,c. Xylenes 29a,b,c. Dimethylacetamide 30. N-Methylpyrilidone Page 46 23

24 Solvents in Drugs Page Helium to Hydrogen saves an additional 5 minutes (33%) Page 48 24

25 Resolution Check min 10 min Page 49 Drugs of Abuse Page 50 25

26 Drugs of Abuse HP-5ms 1. Bupropion 2. Bupropion metabolite 1 ml/min 100 o C (1 min) 20 o C/min to 300 o C (5 min) 3. Diphenhydramine 4. Amitriptyline 5. Citalopram 6. Ethyl morphine 2 ml/min 100 o C (0.5 min) 30 o C/min to 300 o C (3 min) Page 51 Conclusions USING Higher gas velocities Hydrogen as carrier gas (instead of Helium) Smaller I.D./shorter length columns Method Translation Software (properly adjusts temperature programs) ALLOWS Page 52 26

27 Fast Track to Fast GC Input the original column dimensions, method parameters and if the situation is... Same Carrier Gas Type Same Column Try Higher Gas Velocity Same Column Try Different Carrier Gas Type (Helium to Hydrogen) Same Carrier Gas Type Try Smaller Column i.d. (or other new dimensions) Step 1 Input same column dimensions. If below OPGV, click fast analysis mode. In translate only mode, click on Hydrogen carrier and set instrument parameters with new velocity and temperature program. In translate only mode input new column dimensions and set instrument with new velocity and temperature program. Step 2 Step 3 If above OPGV, click none mode and input 10-20cm/sec faster than currently using. Look at new temperature program given and evaluate if equipment is capable of performing it. If instrumentation can perform new conditions, run and evaluate chromatogram for resolution. Evaluate chromatogram for resolution. If plenty of resolution, click on none mode and try higher velocities until resolution is not adequate. Evaluate chromatogram for resolution. If plenty of resolution, click on none mode and try higher velocities until resolution is not adequate. If instrumentation can t perform new conditions try a slower velocity until the instrument can perform the new conditions. Page 53 Acknowledgements Lisa Wool of EPA Houston Raquel Olegario of Philip Morris Colleen DuRousseau of Exxon-Mobil Andy Clausen of Merck Al Elian of MA State Police Crime Lab YOUR NAME HERE Page 54 27

28 TECHNICAL SUPPORT , #3, #3, # (Daron) (toll free) Page 55 28