SPE-DEX 3100 Solid Phase Extraction System

Transcription

1 SPE-DEX 3100 Solid Phase Extraction System Service Manual

2 16 Northwestern Drive, Salem, NH Telephone: (603) Toll-Free: (800) USA Only Website: Copyright 2016 by Horizon Technology, Inc. All rights reserved. Horizon Technology, Inc. reserves the right to change the information in this document without notice. No part of this work may be processed, reproduced, or transmitted in any form or by any means, electronic or mechanical, including photocopying and recording, or by any information storage or retrieval system, except as may be expressly permitted in writing by Horizon Technology, Inc. SPE-DEX is a trademark of Horizon Technology, Inc. All other product names herein are used for identification purposes only and are recognized as properties (including trademarks, registered trademarks, and referenced copyrighted materials) of their respective holders. P/N: REV A (September 2016) 1

3 Contents Preface... 4 Manual Audience and Intent... 4 Technical Support Nomenclature Recommended System Maintenance Daily Maintenance Weekly Maintenance Troubleshooting Preventative Maintenance Kits and Tools Preventative Maintenance Procedures Sensor Height Adjustment Procedure ( ) Sensor Installation Procedure ( , ) Check Valve Installation Procedure ( ) Water Inlet Valve Rinse Rod Replacement ( ) Platform Vacuum Fitting Replacement ( ) Using the Hydrophilic Solution ( ) Waste Fitting Replacement ( ) Service Kits and Parts List Service Replacement Procedures Prewet or Rinse Valve Installation Procedure ( ) Water Waste Valve Installation Procedure ( ) Sample Collect Valve Installation Procedure ( ) Solvent Waste Valve Installation Procedure ( ) Water Inlet Valve Actuator Installation Procedure ( ) Collection Adapter Installation Procedure ( ) Circuit Board Installation Procedure ( ) Exhaust Fan Installation Procedure ( ) Pressure Regulator Installation Procedure ( ) Schematics Nitrogen and Solvent Delivery System Waste and Vacuum System

4 8.3. Internal Plumbing Schematic Available Service Documents

5 Preface Manual Audience and Intent This manual, which is intended for all service technicians with Horizon Technology product service training, provides the information needed to perform maintenance on the SPE-DEX The information may contain typographical errors or technical inaccuracies and is subject to change without notice. Modifications or enhancements may also be made to the product at any time. For the most current information, consult Horizon Technical Support. Product Safety The SPE-DEX 3100 is designed with operator safety in mind. However, the product use is at the discretion and risk of the operator or laboratory supervisor/manager. Do not attempt to disassemble or repair the unit before reviewing this guide. It is recommended that the SPE-DEX 3100 only be serviced by trained service technicians and with factory approved components. Electro-Static Discharge To reduce the risk of electrical shock, an Electro-Static discharge mat should be used when troubleshooting or repairing the SPE-DEX Personal Protective Equipment Due to the nature of use for the SPE-DEX 3100, it is recommended that proper Personal Protective Equipment (PPE) be used when servicing it. This should include safety glasses and gloves, but may include additional PPE based on your safety requirements. Technical Support Visit the Horizon website for technical information in addition to that provided in this guide: If you have questions about the SPE-DEX 3100 solid phase extraction system that are not fully addressed in this guide or our website, please contact the Horizon Technology Technical Customer Support Center: Phone: (603) Fax: (603) Customer Support Center hours are Monday through Friday, 8:00 a.m. to 5:00 p.m. EST. The Center provides expert technical support including troubleshooting, repair instructions, service and installation scheduling, and replacement part information. 4

6 Notes: 5

7 1. Nomenclature Throughout this document, the following terms will be used which require definition: Preventative Maintenance (PM): A PM is preformed yearly and is designed to maintain the instrument through the measurement of various conditions and the replacement of commonly wearable parts. 6

8 2. Recommended System Maintenance 2.1. Daily Maintenance On a daily basis when the instrument is in routine operation: Clean up any spills immediately using a soft, dry cloth. Follow that by wiping the area with a cloth made damp with a mild nonabrasive general purpose laboratory cleaning solution. Do not spray a cleaning solution directly onto the instrument. Flush the Water-Waste, Solvent-Waste, and Elution vacuum pathways using warm water and the Clean System method. Clean accessory components like Disk Holders, Cap Adapters, and Collection Vessels using a mild nonabrasive general purpose laboratory cleaning solution. Follow this cleaning with a rinse of the final elution solvent for best results. Ensure that all gaskets are in place and in good condition. Verify that the Water-Waste, Solvent-Waste, and Hexane/Methanol canisters are free from defects. If needed, replace them prior to placing them under pressure or vacuum Weekly Maintenance When the instrument is in routine operation: Check the gas supply and tubing to ensure that all of the fittings are properly seated and that the tubing is not damaged in any way. Check the solvent tubing and ensure that all fittings are properly seated and there is no damage. Check the exhaust ducting and ensure that the attachments at both ends are secure and free of obstruction. Verify that the ducting is not crimped or crushed by other instruments or heavy objects. Flush the Water-Waste, Solvent-Waste, and Elution vacuum pathways using warm water and the Clean System method. 7

9 3. Troubleshooting SYMPTOM PROBABLE CAUSE SOLUTION Extractor does not power on. Power not supplied to the Supply power to the Controller. Controller. Inline Transformer not connected to Power Cord. Connect the Power Cord to the Inline Transformer. Controller not connected to extractor. Connect the Data Cable to the Extractor from the Controller. Controller displays message Extractor Communication Lost. Data Cable not attached. Ensure that the Data Cable is properly seated on both the Extractor s and the Controller s Data Port. Bad Data Cable. Replace the Data Cable. Prewet solvent does not dispense. Unfamiliar method. Low/No pressure. Low/No solvent in connected bottle. Kinked Solvent Delivery line Valve not actuating. 8 Confirm method steps to ensure that the prewet is scheduled. Confirm that the method has been loaded onto the extractor in use. Verify that nitrogen source is set correctly. Verify that the Solvent Canister Pressure is set correctly. Verify that all nitrogen lines are free from kinks. Verify that the Canister cap is tight and that the gasket is present and undamaged. Verify that enough solvent is contained within the Solvent Canister. Inspect all Solvent Delivery lines, replace if needed. Replace valve. Prewet solvent is not drained. Insufficient vacuum. Verify that the vacuum source is set correctly. Verify that the Water-Waste Carboy Manifold is correctly seated. Close the Water-Waste Carboy Drain. Verify the Solvent Waste vacuum is set correctly. Prewet solvent is drained immediately. Solvent-Waste valve is not opening. Waste valve is stuck open. Write a short method with only 1 prewet in it and run. Replace valve if no actuation is seen. Place DI Water into Disk Holder attached to a Station. Watch the

10 SYMPTOM PROBABLE CAUSE SOLUTION waste containers and identify which one the water is pulled into. Check Valve is stuck open. Use the Check Valve Tool to cycle the Check Valve. Place DI Water into a Disk Holder attached to a Station. Replace Check Valve if necessary. Prewet solvent drips from the Prewet Arm. Prewet valve stuck open Attempt to flush the valve by writing a method which dispenses that solvent and run it numerous times. Replace the valve if this does not work. Sample does not dispense. Low/No pressure. Verify that the nitrogen source is set correctly. Ensure that the internal pressure reading on the left gauge reads 50 psi. Water Inlet Valve Actuator is stuck closed. Water Inlet valve needs to be treated (shaking the valve allows sample to flow through for a limited time). Actuate the Water Inlet Valve manually. Treat the valve using Hydrophilic Solution. Sample overflows. Air leak. Ensure that all cap adapters contain an appropriate gasket which is undamaged. Verify that the sample bottle being used does not have defects on the threads or the rim. Replace the Cap Adapter. Sample flows slowly or not at all. Insufficient vacuum. Verify that the vacuum source is set correctly Verify that the Water-Waste Carboy Manifold is correctly seated. Close the Water-Waste Carboy Drain. Insufficient prefilters used. Use additional prefilters to increase the flow rate through the SPE Disk. Disk Holder Support Screen is off-center. Open the disk holder and verify that the imprint of the Support Screen on the bottom of the SPE disk is off-center. 9

11 SYMPTOM PROBABLE CAUSE SOLUTION Water-Waste valve is not opening. With no disk in the disk holder, run DI Water using Drain Sample method to confirm that the valve is not firing and needs replacement. Water Waste line is kinked Verify that all waste lines are System advances to Air Dry without having processed the entire sample. System does not advance to Air Dry. Fluid leaking from between the Water Inlet Valve and the Station Arm. Slow sample flow rate through SPE disk. Bad Thermistor. Thermistors remained covered. Rinse Rod O-Rings need replacement. Rinse Rod needs replacement. free from kinks. Verify Sensor heights using the Sensor Height Setting Tool. Investigate other prefiltering options. Verify Sensor heights using the Sensor Height Setting Tool. Ensure that Sensor is connected to the Control Circuit Board correctly. Raise the Prewet Arm so it is not touching anything within the Disk Holder. Replace the O-Rings. Replace the Rinse Rods using the appropriate Service Kit Rinses not dispensed. Unfamiliar method. Confirm method steps Confirm that the method has been loaded onto the station in use. Low/No pressure. Low/No solvent in connected Canister. Solvent Monitor Not Reset Kinked delivery line. Verify that nitrogen source is set correctly. Verify that the Solvent Canister pressure gauges read between 13 and 16 psi. Tighten Canister lids if it does not. Verify that all nitrogen lines are free from kinks. Verify that the pressure reading on the right gauge internally is between 14 and 17 psi. Verify that enough solvent is contained within the Canister. Reset Solvent Monitor to indicate that there is solvent in the attached Canisters. Inspect all lines. 10

12 SYMPTOM PROBABLE CAUSE SOLUTION Valve not actuating. Ensure that the connecter is properly attached to Main Circuit Board. Fluid drips into the Collection Vessel. Check Valve needs cleaning. Use Check Valve Tool to free Check Valve Fill the Disk Holder with warm water and run Drain Elute. Check Valve needs to be Replace Check Valve. replaced. Low/No Elution volume. Unfamiliar method. Confirm method steps. Confirm that the method has been loaded onto the station in use. Check Valve is stuck. Rinse solvent was dispensed, but not pulled into the Disk Holder. Use Check Valve Tool to free Collection Vessel improperly attached. Insufficient vacuum. Waste valve is stuck open. Check Valve. Attach Collection Vessel to Collection Adapter using an upwards-twisting motion to ensure a good seal. Verify that the vacuum source is set correctly. Verify that the Water-Waste Carboy Manifold is correctly seated. Close the Water-Waste Carboy Drain. Verify the Solvent Waste bottle is closed. With the extractor idle, fill a disk holder with water and verify that the fluid level does not decrease. If it does, examine the Waste vessels to determine which valve is stuck open and replace that valve. 11

13 4. Preventative Maintenance Kits and Tools This is a list of the standard preventative maintenance part and kits that can be purchased from Horizon Technology. To ease the process of determining which parts are needed to perform a yearly preventative maintenance (PM), there are two pre-built kits available: The PM Kit with PN is designed for those customers who use binder-less SPE disks to perform their extractions such as Horizon s Pacific Premium disks. The PM Kit with PN is designed for those customers who do not use binder-less SPE disks for their extractions. The latter of the two kits contains more fittings and tubing to ensure that any binder which may have deposited within them is removed and the vacuum is kept at an optimal level. Some of the parts contained within the PM Kits are available by themselves if needed, however many of the individual parts are not. In addition, there are certain tools which are needed in the daily running of the SPE-DEX 3100 or in the diagnoses of problems with the instrument s internal pathways. This section will list the applicable tools for re-order purposes. Part # Description Sensor Height Adjustment Tool Liquid Level Thermistor 12

14 Overflow Thermistor Check Valve Tool Check Valve Vacuum Gauge Kit This tool allows for the measurement and analysis of the condition of the subsystems for a SPE-DEX 4790 system & Vacuum Gauge 3100 Adapter Allows for the Vacuum Gauge Kit to be used on a SPE-DEX Hydrophilic Solution 13

15 Solvent Rod Waste Fittings Platform Vacuum Fitting Solvent Rod O-Ring Internal (Pack of 100) Solvent Rod O-Ring External (Pack of 100) Water Inlet Valve Gasket - External NA NA Water Inlet Valve Gasket - Internal Disk Holder O-Ring Water-Waste Bottle Manifold NA Solvent-Waste Bottle Manifold Vacuum Line Kit 14

16 Waste Lines SPE-DEX 3100 PM Kit for Pacific Premium Disks This kit is designed to service a SPE-DEX 3100 that uses a binder-free SPE disk. o Solvent Waste Bottle Gasket o Check Valve Tool o Liquid Level Sensor Kit (3 Included) o Sensor Height Adjustment Tool 15

17 o Hydrophilic Solution o Collection Adapter O-Rings (9 Total, 3 of each Size) o Check Valve (3 Included) o Check Valve Screws (3 Sets of 3 Screws Included) o Collection Adapter Screws (3 Sets of 2 Screws Included) o Prewet Arm Nylon Screws (6 Included) o Waste Fittings o Waste Lines 16

18 o Vacuum Line Kit SPE-DEX 3100 PM Kit for Non-Pacific Premium Disks This kit is designed to service a SPE-DEX 3100 that does not use a binder-free SPE disk. o Check Valve Tool o o o Water-Waste Internal Tubing (15 feet Included, 1/8 inch ID, 3/16 inch OD) Solvent Waste Internal Tubing (30 feet Included, 1/16 inch ID, 1/8 inch OD) Liquid Level Sensor Kit (3 Included) NA NA o Sensor Height Adjustment Tool o Hydrophilic Solution 17

19 o Collection Adapter O-Rings (9 Total, 3 of each Size) o Check Valve (3 Included) o Check Valve Screws (3 Sets of 3 Screws Included) o Collection Adapter Screws (3 Sets of 2 Screws Included) o Prewet Arm Nylon Screws (6 Included) o Headless Nut & Ferrules (9 Included) o Water & Solvent Waste T-Fitting (3 Included) o Platform Waste Vacuum Fitting (3 Included) o Waste Fittings 18

20 o Solvent Waste Bottle Manifold o Water Waste Bottle Manifold o Waste Lines o Vacuum Line Kit 19

21 5. Preventative Maintenance Procedures 5.1. Sensor Height Adjustment Procedure ( ) WORK INSTRUCTION: Rev A 02/22/2016 PURPOSE: The purpose of this procedure is to provide instruction on the use of the Sensor Height Adjustment tool to adjust either the Overflow or the Liquid Level Sensor on the SPE-DEX TOOLS: - SPE-DEX 3100 Sensor Height Adjustment Tool Inch Hex (Allen) Wrench PARTS REQUIRED: SPE-DEX 3100 Sensor Height Adjustment Tool PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. Liquid Level Sensor Overflow Sensor 20

22 1. Position the Sensor Height Adjustment Tool next to the Prewet Arm. The Liquid Level Sensor should be able to pass over the bottom tier (labeled with the word Load) on the Tool and just barely touch the surface. NOTE: For the correct adjustment of the Sensor, the Sensor Height Adjustment Tool should be sitting on the raised portion of the Platform, next to the connection point for the Disk Holder. 2. If needed, using a 0.05 inch hex (Allen) wrench, loosen the set screws in the Prewet Arm that hold the Liquid Level Sensor in place and adjust the sensor to the correct height. When the correct height is achieved, re-tighten the screws. Liquid Level Sensor Set Screws 3. The Overflow sensor has two positions, one for a 47 mm Disk Holder and one for a 90 or 100 mm Disk Holder. Position the Sensor Height Adjustment Tool next to the Prewet Arm. The Overflow Sensor should be able to pass over the tier labeled with the applicable Disk Holder on the Tool and just barely touch the surface. NOTE: For the correct adjustment of the Sensor, the Sensor Height Adjustment Tool should be sitting on the raised portion of the Platform, next to the connection point for the Disk Holder. 21

23 For 90 or 100 mm Disk Holders For 47 mm Disk Holders 4. If needed, using a 0.05 inch hex (Allen) wrench, loosen the set screws in the Prewet Arm that hold the Overflow Sensor in place and adjust the sensor to the correct height. When the correct height is achieved, re-tighten the screws. Overflow Sensor Set Screws 5. End of procedure. 22

24 5.2. Sensor Installation Procedure ( , ) WORK INSTRUCTION: Rev A 02/05/2016 PURPOSE: The purpose of this procedure is to provide instruction for the replacement either the Overflow or the Liquid Level Sensor on the SPE-DEX TOOLS: - ¼ Inch Nut Driver/ Socket Wrench - Medium Flat Blade Screwdriver (Optional) Inch Hex (Allen) Wrench - 5/32 Inch Hex (Allen) Wrench - Small Phillips Head Screwdriver - Medium Phillips Head Screwdriver - Wire Cutters PARTS REQUIRED: Overflow Sensor Service Kit Contains: (1) Overflow Sensor with Terminations (1) Connector Housing OR Liquid Level Sensor Service Kit Contains: (1) Liquid Level Sensor with Terminations (1) Connector Housing SPE-DEX 3100 Sensor Height Adjustment Tool PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, drain the solvent from the internal lines and disconnect all external lines going into the instrument. 1. Move the instrument to a location where both the rear and the front of the instrument are easily accessed. 2. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the (11) screws from the rear cover of the instrument. 23

25 3. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in and remove the Exhaust Fan wire from the Main Circuit Board. 4. Using a 0.05 inch hex (Allen) wrench, loosen the set screws in the Prewet Arm that hold the Sensor being replaced in the correct location. There are (3) screws that retain the Liquid Level Sensor and (2) that hold the Overflow Sensor. Liquid Level Sensor Set Screws Overflow Sensor Set Screws 5. Using a small Phillips head screwdriver, loosen the (2) plastic screws on the top of the Prewet Arm. 24

26 6. Slide the Sensor up and out of the Prewet Arm. 7. Using a pair of wire cutters, cut and remove the wire ties holding the Sensor Wires to the Prewet Tubing under the front Platform. Be careful not to cut any of the wires or tubing when removing the wire ties. 8. Inside of the instrument, identify the correct Sensor wire: Label Connector Station 1: Overflow 11 J1 Station 1: Liquid Level 1 J4 Station 2: Overflow 12 J2 Station 2: Liquid Level 2 J5 Station 3: Overflow 13 J3 Station 3: Liquid Level 3 J6 25

27 Liquid Level Sensors Overflow Sensors 9. Unplug the Sensor which will be changed and clip the connector off using the wire cutters. 10. Follow the Sensor Wire Bundle and clip off any wire ties using the wire cutters. 11. If needed, use a medium Phillips screwdriver and a 5/32 inch hex (Allen) wrench to remove the Wire Organizers from the Sensor Wire Bundle. For newer units, the Wire Bundle will slide out of the Wire Organizer if pulled upwards. 12. Pull the Sensor Wire out through the front of the instrument and up the Prewet Arm Pole. 13. Install the replacement Sensor in the correct location, using the Sensor Height Adjustment Tool to set the correct height. Tighten the set screws on the Prewet Arm when the correct height is achieved. 14. Push the end of the Sensor Wire down, through the Prewet Arm Pole being careful not to damage the connectors. 15. Push the Sensor Wire under the plastic screws on the Top of the Prewet Arm and tighten to hold the Wire in place. 16. Under the platform, push the Sensor Wire through the hole containing the remaining Sensor Wire and the two Prewet Solvent Lines being careful not to damage the connectors. 17. Apply wire ties to the external Sensor Wires and the Prewet Solvent Line. Generally (3) to (4) is best. 18. Inside the instrument, run the Sensor Wire through the same pathway as the previous Sensor Wire and secure using Wire Organizers that were previously removed. 19. Slide the connector into the Plastic Connecter Housing that came with the Service Kit, apply the appropriate label to the connector, and install onto the Main Circuit Board. 20. Re-install the cover onto the chassis being sure to connect the Exhaust Fan to the Main Circuit Board. 21. End of procedure. 26

28 5.3. Check Valve Installation Procedure ( ) WORK INSTRUCTION: Rev D 04/28/2016 PURPOSE: The purpose of this procedure is to provide instruction on how to replace the Check Valve on a SPE-DEX 1000XL, SPE-DEX 3000XL, SPE-DEX 3100, and SPE-DEX TOOLS: - Small Flat Head Screwdriver - Pliers PARTS REQUIRED: Check Valve Service Kit PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. 1. Locate the Check Valve on the instrument in question: 1000XL 3000XL

29 2. Looking upwards at the Check Valve, remove the three screws which attach the Check Valve to the Collection Adapter and set aside for re-use Front 1000XL Front 3000XL 3100 Rear Rear NOTE: Prior the removal of the screws, note the position of the Vacuum Port on the Check Valve. 3. Using pliers, pull downwards on the Check Valve to remove it from the Collection Adapter. 4. Install the new Check Valve in the Collection Adapter being sure to line the Vacuum Port up correctly (see the above pictures). NOTE: If the Vacuum Port is not correctly aligned, the elution of solvent from the Disk Holder to the Collection Vessel will not be possible. 5. Install the (3) screws which were previously removed being careful not to overtighten. 6. Use the appropriate Check Valve Tool to ensure that the internal valve moves freely. 7. End of procedure. 28

30 5.4. Water Inlet Valve Rinse Rod Replacement ( ) WORK INSTRUCTION: Rev A 03/29/2016 PURPOSE: The purpose of this procedure is to provide instruction for replacing the rinse rods in a 3100 water in valve. PARTS REQUIRED: Valve Service Kit Contains: (2) new rinse rod assemblies (with o-rings) (2) replacement spring pins (1) pin removal tool (2) 5/32 diameter check ball PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. 1. Using Pin Removal Tool gently push the Spring Pin out of valve body and discard 2. Remove Rinse Rod and Check Ball. Discard both 3. Insert new Check Ball on valve body 4. Orient new Rinse Rod with square o-ring toward valve body and insert onto valve 5. Push new Spring Pin into pin hole 6. Utilizing Pin Tool carefully push Spring Pin into valve body until flush with surface 7. Repeat for other Rinse Rod. 8. End of procedure. Rounded end Square 29

31 5.5. Platform Vacuum Fitting Replacement ( ) WORK INSTRUCTION: Rev A 06/24/2016 PURPOSE: The purpose of this procedure is to provide instruction for the replacement of the Platform Vacuum Fittings on a SPE-DEX TOOLS: - ¼ Inch Nut Driver - 3/16 Inch Hex (Allen) Wrench - 7/16 Inch Nut Driver - 7/16 Inch Open-Ended Wrench - Wire Cutters PARTS REQUIRED: Platform Vacuum Fittings Service Kit Contains: (3) Platform Vacuum Fittings PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, drain the solvent from the internal lines and disconnect all external lines going into the instrument. 1. Move the instrument to a location where both the rear and the front of the instrument are easily accessed. 2. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the (11) screws from the rear cover of the instrument. 3. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in and remove the Exhaust Fan wire from the Main Circuit Board. 30

32 4. Inside the instrument, use a 3/16 Inch Hex (Allen) Wrench to loosen and remove the (4) bolts. 5. On the outside of the instrument, under the front platform, locate and remove the (4) bolts holding the Platform to the instrument using the 7/16 Inch Nut Driver. 6. Gently pull the Platform away from the metal chassis of the instrument to expose the Platform Vacuum Fittings. 31

33 7. Using the wire cutters, cut the tubing as close to the Vacuum Fitting as possible. NOTE: When cutting the tubing, ensure that all cuts are made square across the width of the tubing. Uneven cuts may result in vacuum loss at the fitting later. 8. Using the 7/16 Inch open-ended wrench, unscrew the Platform Vacuum Fitting from the Platform. 9. Install the replacement Vacuum Fitting into the Platform. NOTE: Do not over-tighten the Vacuum Fitting as this may strip the threads from the Platform itself and require that the entire Platform be replaced. 10. Attach the Vacuum Line onto the Vacuum Fitting. 11. Gently push the Platform backwards until it touches the chassis. 12. Inside the instrument, re-install the (4) bolts that hold the Platform onto the instrument. Make sure that each bolt has (1) washer on it. NOTE: Do not over-tighten the bolts as this may strip the threads from the Platform itself and require that the entire Platform be replaced. 13. Re-install the (4) bolts under the Platform making sure that each bolt has a single washer on it. 14. Re-install the cover onto the chassis being sure to connect the Exhaust Fan to the Main Circuit Board. 15. End of procedure. 32

34 5.6. Using the Hydrophilic Solution ( ) WORK INSTRUCTION: Rev A 06/24/2016 (excerpt) PURPOSE: The purpose of this procedure is to provide instruction on coating the Water Inlet Valve components on SPE-DEX systems using Hydrophilic Solution. TOOLS: - Tape (SPE-DEX 1000XL, 3000XL, and 3100) PARTS REQUIRED: Hydrophilic Solution Contains: (1) Hydrophilic Solution, 250 ml (3) Plastic Vials (1) MSDS PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. 1. Place a piece of tape over the bottom of each Rinse Rod on the Water Inlet Valve which will be treated. 2. Inserting the Downtube into a Plastic Vial and place the assembly onto one of the Bottle Holders on the instrument. 33

35 3. Open the Water Inlet Valve by rotating the Driveshaft fully clockwise. 4. Fill the valve to the top with Hydrophilic Solution. 5. Open and close the valve using the Driveshaft to ensure that all parts are coated. 6. Leave the valve open part of the way and allow it to soak for at least 2 hours. 7. After the two hours, discard the used Solution and remove the tape from the valve. 8. Flush the valve use DI Water while opening and closing the valve to ensure that all wetted areas have been rinse. 9. Using the Water Inlet Valve, run at least (2) Purges of the instrument to ensure that all wetted areas have been rinsed. 10. End of procedure. 34

36 5.7. Waste Fitting Replacement ( ) WORK INSTRUCTION: Rev A 05/20/2016 PURPOSE: The purpose of this procedure is to provide instruction for the replacement of the Solvent and Water Waste Fittings on a SPE-DEX TOOLS: - ¼ Inch Nut Driver/ Socket Wrench - ¾ Inch Box Wrench - 5/8 Inch Box Wrench - Wire Cutters PARTS REQUIRED: Waste Fittings Service Kit Contains: (1) Water Waste Fitting Assembly (1) Solvent Waste Fitting Assembly PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, drain the solvent from the internal lines and disconnect all external lines going into the instrument. 1. Move the instrument to a location where both the rear and the front of the instrument are easily accessed. 2. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the (11) screws from the rear cover of the instrument. 3. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in and remove the Exhaust Fan wire from the Main Circuit Board. 35

37 4. Using a ¾ Inch Wrench, loosen and remove the nut on the Water Waste Fitting and pull into the open space in front of the Regulator for easy access. 5. Using a 5/8 Inch Wrench, loosen and remove the nut on the Solvent Waste Fitting and pull into the open space in front of the Regulator for easy access. 6. Using a pair of Wire Cutters, cut through the tubing attached to both the Solvent Waste manifold and the Water Waste manifold. NOTE: When cutting the tubing, ensure that all cuts are made square across the width of the tubing. Uneven cuts may result in vacuum loss at the fitting later. 36

38 7. Install the tubing onto the new Waste Assemblies pressing tight to ensure a good seal. If needed, place the tubing into a beaker of warm water for seconds to make the material more pliable before attempting to push the tube onto the barb. NOTE: The position of the tubing on each of the manifolds does not matter. 8. Re-install each of the two assemblies onto the chassis making sure to place to included lock-washer on each of the assemblies before pushing it through the chassis from the inside. 9. Place the appropriate nut onto each of the assemblies and tighten using the correct wrench. 10. End of procedure. 37

39 6. Service Kits and Parts List This is a list of the service kits that can be purchased from Horizon Technology. Part # Description Prewet Or Rinse Valve Water Waste Valve Sample Collect Valve Solvent-Waste Valve Water Inlet Valve Actuator 38

40 Collection Adapter Interface Circuit Board Replacement Fan Pressure Regulator Kit Water Inlet Valve Solvent-Waste & Elution Tubing (Sold Per Foot) 1/16 inch ID, 1/8 inch OD Water-Waste Internal Tubing (Sold Per Foot) 1/8 inch ID, 3/16 inch OD 39

41 7. Service Replacement Procedures Due to the nature of use for the SPE-DEX 3100, it is recommended that proper Personal Protective Equipment (PPE) be used when servicing the System. This is to include safety glasses and gloves, but may include additional PPE based on your safety requirements Prewet or Rinse Valve Installation Procedure ( ) WORK INSTRUCTION: Rev B 06/01/2016 PURPOSE: The purpose of this procedure is to provide instruction on replacing a Prewet or Rinse valve on a SPE-DEX 3000XL or SPE-DEX TOOLS: - ¼ Inch Nut Driver/ Socket Wrench - Medium Flat Blade Screwdriver (Optional) - Needle Nose Pliers - Pick - Wire Cutters PARTS REQUIRED: Prewet or Rinse Valve Service Kit Contains: (1) Prewet or Rinse Valve with Terminations (2) Gaskets PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, drain the solvent from the internal lines and disconnect all external lines going into the instrument. 1. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the screws that hold the rear cover on. 2. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in and remove the Exhaust Fan wire from the Main Circuit Board. 40

42 3. Identify the correct valve: Front Station 3 Station 2 Station 1 Rear Front Rear 4. Using the Needle Nose Pliers, loosen the white nut at the bottom of the valve. 5. Using the Wire Cutters, remove any wire ties from the valve s wires and unplug the valve from the Circuit Board. 41

43 NOTE: Extra care should be taken to prevent damaging the wires contained within any wire tie within the SPE-DEX system when cutting them off. It is recommended to cut through the head of the tie and to pull apart the clip to unbundle the wires. 6. With the valve removed, ensure that the black sealing gaskets are removed from within the valve manifold block. If they are not, use the Pick to remove them from within the manifold block. 7. Install the new gaskets into the valve manifold block. It is helpful to use the Pick to install the replacement gaskets. 8. Install the replacement valve into the manifold block and ensure that the wires are extended in the same direction as the other valves in the block. 9. Plug the wire connector into the appropriate position on the Circuit Board. Valve Wire Label Circuit Board Connections Station 1 Station 2 Station 3 Prewet 1 1 J8 J17 J26 Prewet 2 2 J9 J18 J27 Rinse 1 5 J13 J22 J31 Rinse 2 6 J14 J23 J Bundle the wires together using a wire tie (not included). 11. Reinstall the rear cover ensuring that the fan is plugged into the Circuit Board. 12. End of procedure. 42

44 7.2. Water Waste Valve Installation Procedure ( ) WORK INSTRUCTION: Rev F 04/26/2016 PURPOSE: The purpose of this procedure is to provide instruction on the replacement of a Water Waste Valve on a SPE-DEX TOOLS: - ¼ Inch Nut Driver/ Socket Wrench - 11/32 Inch Nut Driver/ Socket Wrench - Wire Cutters PARTS REQUIRED: Water Waste Valve Service Kit PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, drain the solvent from the internal lines and disconnect all external lines going into the instrument. 1. Move the instrument to a location where the rear of the instrument is easily accessed. 2. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the rear cover: a. For a SPE-DEX 1000XL, remove the (8) screws. b. For a SPE-DEX 3000XL, remove (14) screws. c. For a SPE-DEX 3100, remove (11) screws. 3. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in. a. On a SPE-DEX 3000XL or 3100, remove the Exhaust Fan wire from the Main Circuit Board. b. On a SPE-DEX 1000XL, separate the wiring at the connector. 43

45 4. Looking inside the instrument, locate the appropriate Water Waste Valve. NOTE: A SPE-DEX 1000XL has with only (1) of the valves which are pointed out in the below picture. Front Station 3 Valve Station 2 Valve Station 1 Valve Rear 5. Remove the two 11/32 inch nuts that secure the Valve to the Panel. 6. Remove the black Nitrogen Tubing from the Valve by pulling it off of the valve. Trim approximately 1/8 inch (3 mm) off of the Nitrogen Tubing. 7. Looking below the Valve Panel, locate the bottom of the afflicted Water Waste Valve and the stainless steel T-fitting. 44

46 Station 3 Valve Station 2 Valve Station 1 Valve NOTE: The SPE-DEX 1000XL has only (1) set of the tubing pointed out in the above picture. To: Solvent Waste Valve To: Bulkhead Fitting From: Disk Holder (Flow directionality is noted in Green) 8. Using the wire cutters, cut through the lines connected to the T-fitting and remove the Valve from the instrument. NOTE: It may be necessary to remove wire ties to free up the tubing so it can be cut. If this is necessary, it is recommended that the head of the wire tie be cut off to ensure that the tubing is not unnecessarily punctured. 45

47 Cut Here 9. Lift up on the valve and cut through the remaining Waste line. 10. Remove the Water Waste Valve. 11. Using the replacement valve, attach the Waste line that was removed in step 9 to the barb located on the side of the valve labeled SMC. 12. Set the replacement valve in place feeding the stainless steel T-fitting through the hole in the valve panel. NOTE: On a SPE-DEX 1000XL, it is necessary to turn the top of the valve to ensure that the nitrogen supply tube can be attached. 13. Attach the black Nitrogen Tubing to the top plastic fitting on the Water Waste Valve. 14. Below the valve panel, attach the large diameter tubing which comes from the Disk Holder to the lower barb on the T-fitting. 15. Attach the small diameter tubing which goes to the Solvent Waste Valve to the remaining barb on the T-fitting. 16. Re-install the two 11/32 nuts to secure the Water Waste Valve to the Valve Panel. 17. Re-install the cover onto the chassis being sure to connect the Exhaust Fan to the Main Circuit Board or the wire bundle at the connector. 18. End of procedure. 46

48 7.3. Sample Collect Valve Installation Procedure ( ) WORK INSTRUCTION: Rev B 06/03/2016 PURPOSE: The purpose of this procedure is to provide instruction for the replacement of the Sample-Collect Valve in a SPE-DEX 3000XL or SPE-DEX TOOLS: - ¼ Inch Nut Driver/ Socket Wrench - Small Flat Blade Screwdriver (SPE-DEX 3000XL) - Small Phillips Head Screwdriver (SPE-DEX 3100) - Needle-Nose Pliers - Wire Cutters PARTS REQUIRED: Sample-Collect Service Kit Contains: (1) Sample-Collect Valve (1) Connector Housing PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, drain the solvent from the internal lines and disconnect all external lines going into the instrument. 1. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the screws that hold the rear cover on. 2. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in and remove the Exhaust Fan wire from the Main Circuit Board. 47

49 3. Identify the correct valve: Front Station 3 Station 2 Station 1 Rear 4. Under the Valve Panel, remove the two screws that hold the valve to the Valve Panel: 5. Remove the two tubing connectors from the valve using the needle-nose pliers: 48

50 6. Follow the green electrical wires and unplug the wire from the Circuit Board; remove any wire ties which may be on the wires. NOTE: Extra care should be taken to prevent damaging the wires contained within any wire tie within the SPE-DEX system when cutting them off. It is recommended to cut through the head of the tie and to pull apart the clip to unbundle the wires. 7. Using the replacement valve, plug the electrical connection into the Circuit Board. NOTE: The electrical connection for the Sample-Collect Valves on the Circuit Board are all labeled as 0. Station 1 connects to J15 Station 2 connects to J24 Station 3 connects to J33 8. Attach the two tubing fittings to the replacement valve ensuring that: The NO port is open. The NC port has the tubing which extends to the Solvent Waste Bulkhead Fitting. The COMM port has the tubing which extends to through the front chassis of the instrument. 9. Install the two screws through the Valve Panel into the bottom of the replacement valve. 10. Reattach the cover ensuring that the Fan is plugged into the Circuit Board. 11. End of procedure. 49

51 7.4. Solvent Waste Valve Installation Procedure ( ) WORK INSTRUCTION: Rev B 06/06/2016 PURPOSE: The purpose of this procedure is to provide instruction for the replacement of the Solvent-Waste Valve in a SPE-DEX 3000XL or SPE-DEX TOOLS: - ¼ Inch Nut Driver/ Socket Wrench - Small Flat Blade Screwdriver (SPE-DEX 3000XL) - Small Phillips Head Screwdriver (SPE-DEX 3100) - Needle-Nose Pliers - Wire Cutters PARTS REQUIRED: Solvent-Waste Service Kit Contains: (1) Solvent-Waste Valve (1) Connector Housing PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, drain the solvent from the internal lines and disconnect all external lines going into the instrument. 1. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the screws that hold the rear cover on. 2. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in and remove the Exhaust Fan wire from the Main Circuit Board. 50

52 3. Identify the correct valve: Front Station 3 Station 2 Station 1 Rear 4. Under the Valve Panel, remove the two screws that hold the valve to the Valve Panel: 5. Remove the two tubing connectors from the valve using the needle-nose pliers: 51

53 6. Follow the green electrical wires and unplug the wire from the Circuit Board; remove any wire ties which may be on the wires. NOTE: Extra care should be taken to prevent damaging the wires contained within any wire tie within the SPE-DEX system when cutting them off. It is recommended to cut through the head of the tie and to pull apart the clip to unbundle the wires. 7. Using the replacement valve, plug the electrical connection into the Circuit Board. NOTE: The electrical connection for the Solvent-Waste Valves on the Circuit Board are all labeled as 8. Station 1 connects to J10 Station 2 connects to J19 Station 3 connects to J28 8. Attach the two tubing fittings to the replacement valve ensuring that: The INLET port has the tubing which extends to the Solvent Waste Bulkhead Fitting. This is the vacuum supply. The OUTLET port has the tubing which extends below the Valve Panel. 9. Install the two screws through the Valve Panel into the bottom of the replacement valve. 10. Reattach the cover ensuring that the Fan is plugged into the Circuit Board. 11. End of procedure. 52

54 7.5. Water Inlet Valve Actuator Installation Procedure ( ) WORK INSTRUCTION: Rev A 03/03/2016 PURPOSE: The purpose of this procedure is to provide instruction on how to replace the Water Inlet Valve Actuator on the SPE-DEX TOOLS: - ¼ Inch Nut Driver/ Socket Wrench - Medium Flat Blade Screwdriver (Optional) - Medium Phillips Screwdriver - 3/32 Inch Hex (Allen) Wrench PARTS REQUIRED: Water Inlet Valve Actuator PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, drain the solvent from the internal lines and disconnect all external lines going into the instrument. 1. Move the instrument to a location where both the rear and the front of the instrument are easily accessed. 2. On the front of the instrument, locate the Actuator Key for the station which will have its Actuator replaced (all Actuator Keys are circled below). 53

55 3. Using a 3/32 inch Hex Wrench, loosen the set screws on the top and the bottom of the actuator key. 4. Once loosened, pull the Actuator Key off of the Actuator Shaft and set aside for reinstallation at a later time. 5. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the (11) screws from the rear cover of the instrument. 6. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in and remove the Exhaust Fan wire from the Main Circuit Board. 54

56 7. Looking into the instrument from the rear of the unit, locate the Actuator which is in need of replacement (all Actuators are circled below). 8. Remove the black nitrogen supply lines connected to the Actuator by first compressing the orange flange while pushing the nitrogen tubing into the fitting and then, while continuing to compress the flange, pull the nitrogen tubing out. Compress this flange NOTE: It is helpful at this point to mark the top nitrogen tube for later re-installation. A piece of tape is usually sufficient for this purpose. 9. With the nitrogen tubing removed, remove the (4) screws that hold the Actuator onto the chassis. NOTE: The screw used on the lower right of the Actuator is also used to hold a Tubing Clamp in the correct position; while this screw can be removed, it is easiest to leave it within the instrument attached to the plastic Clamp while the repair is being performed. 55

57 10. Remove the Actuator from the instrument by pulling it back enough so that the Actuator Shaft clears the chassis. 11. Using a 3/32 inch Hex Wrench, loosen the set screws on the top and bottom of the Actuator Shaft and remove the Actuator Shaft for re-use. 12. Install the Actuator Shaft onto the new Actuator and tighten the (2) set screws using a 3/32 inch Hex Wrench. 13. Attach the new Actuator to the instrument using the (4) screws which were previously removed. NOTE: Care should be taken to ensure that the new Actuator is installed in the correct orientation. The two nitrogen connections should face towards the right side of the valve as shown below. If the Actuator is installed incorrectly, the Water Inlet Valve may open in the incorrect direction. 56

58 14. To reattach the (2) nitrogen lines to the actuator, push the tubing into the fitting and then, once it has gone in all the way, pull back gently. NOTE: When reattaching the (2) nitrogen lines to the Actuator, ensure that the correct line from the Actuator Pressure Manifold is selected. The Pressure Manifold has (3) valves on it, each with two channels. The valve furthest to the right is used to control the Station 1 Actuator, while the valve furthest to the left controls the Station 3 Actuator. For each valve, there is a front and a rear channel (the rear being the channel closest to the chassis). The front channel (Marked A on the Pressure Manifold)) on all valves should connect to the top nitrogen fitting on the Actuator, while the rear channel (marked Channel B on the Pressure Manifold) should connect to the bottom fitting on the Actuator. Station 3 Station 2 Station 1 Rear Channel (B) Front Channel (A) 15. Re-install the cover onto the chassis being sure to connect the Exhaust Fan to the Main Circuit Board. 16. On the front of the instrument, reinstall the Actuator Key and ensure that the key is able to be rotated from the vertical position shown clockwise 90 degrees. If the correct path is not followed, the Water Inlet Valve may not open correctly. 57

59 Closed Position Open Position 17. End of procedure. 58

60 7.6. Collection Adapter Installation Procedure ( ) WORK INSTRUCTION: Rev A 05/19/2016 PURPOSE: The purpose of this procedure is to provide instruction for the replacement of the Collection Adapter on a SPE-DEX TOOLS: - Medium Phillips Head Screwdriver - ¼ Inch Wrench - Small Needle Nose Pliers (Optional) - Small Flat Blade Screwdriver - Teflon Tape PARTS REQUIRED: Collection Adapter Service Kit Contains: (1) Collection Adapter (1) #10 O-Ring (1) 22 mm O-Ring (1) 1 mm O-Ring 1 mm O-Ring 22 mm O-Ring #10 O-Ring PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. 1. Locate the Collection Adapter which will be changed and look down into the Disk Holder Recess on the Platform. 59

61 2. Remove the two screws using a Medium Phillips Screwdriver. 3. With the screws removed, grab the collection adapter and pull it out of the recess. 4. Using a ¼ inch Wrench, unscrew the stainless steel fitting from the rear of the Collection Adapter. NOTE: The o-rings which are normally attached to the Collection Adapter may remain inside of the Platform when the Adapter is removed. If this occurs, use a small pair of needle-nose pliers to remove the o-rings. 60

62 5. Remove the Check Valve from the Collection Adapter by using a small flat-bladed screwdriver. If desired, set aside for re-installation in the replacement Adapter. 6. Using the new Collection Adapter, push the #10 O-Ring into the Collection Adapter and either install the Check Valve which was previously removed or install a new Check Valve into the Collection Adapter. Vacuum Port NOTE: If the Vacuum Port is not correctly aligned, the elution of solvent from the Disk Holder to the Collection Vessel will not be possible. NOTE: Do not over-tighten the three screws as doing so may strip the threads of the holes on the Collection Adapter. If this occurs, a new Collection Adapter will need to be purchased. 61

63 7. Using Teflon Tape, tape the threads to the stainless steel fitting and reinstall on the Collection Adapter using the ¼ Inch Wrench. 8. Place the 22 mm O-Ring up into the Platform where the Collection Adapter will be installed. 9. Place the 1 mm O-Ring onto the end of the Collection Adapter and push the Adapter up into the Platform recess. 10. Using a Phillips Screwdriver, reattach the Collection Adapter to the Platform. NOTE: Do not over-tighten the two screws as doing so may strip the threads on the Collection Adapter. If this occurs, a new Collection Adapter will need to be purchased. 11. End of procedure. 62

64 7.7. Circuit Board Installation Procedure ( ) WORK INSTRUCTION: Rev A 06/01/2016 PURPOSE: The purpose of this procedure is to provide instruction for the replacement of the Circuit Board within a SPE-DEX TOOLS: - ¼ Inch Nut Driver/ Socket Wrench - Medium Phillips Head Screwdriver - Wire Cutters PARTS REQUIRED: Circuit Board Service Kit Contains: (1) Circuit Board (1) Ethernet Cable PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, disconnect all power/communication lines going to the instrument. 1. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the (11) screws that hold the rear cover on. 2. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in and remove the Exhaust Fan wire from the Main Circuit Board. 63

65 3. Looking inside the instrument, locate and remove the (8) screws that hold the Circuit Board to the Chassis of the SPE-DEX With the older Circuit Board loose, move the electrical connectors onto the new Circuit Board. To accomplish, place the new Circuit Board in as illustrated below and move one connector at a time. For Reference, a pinout is contained at the end of this document. New Board Old Board 5. Attach the new Circuit Board to the Chassis using the (8) screws removed previously. 6. Reattach the cover being sure to plug the Exhaust Fan into the Circuit Board. 64

66 7. End of procedure. Table 1: Pinout Used Jumper Wire Label Connected-To J1 1,1 Overflow Sensor Station 1 J2 2,1 Overflow Sensor Station 2 J3 3,1 Overflow Sensor Station 3 J4 1 Liquid Level Sensor Station 1 J5 2 Liquid Level Sensor Station 2 J6 3 Liquid Level Sensor Station 3 J8 1 Hexane Prewet Station 1 J9 2 Methanol Prewet Station 1 J10 8 Solvent Waste Station 1 J11 9 Sample Inlet Station 1 J12 7 Sample Waste Station 1 J13 5 Methanol Rinse Station 1 J14 6 Hexane Rinse Station 1 J15 0 Elution Valve Station 1 J16 None Power Indicator LED J17 1 Hexane Prewet Station 2 J18 2 Methanol Prewet Station 2 J19 8 Solvent Waste Station 2 J20 9 Sample Inlet Station 2 J21 7 Sample Waste Station 2 J22 5 Methanol Rinse Station 2 J23 6 Hexane Rinse Station 2 J24 0 Elution Valve Station 2 J26 1 Hexane Prewet Station 3 J27 2 Methanol Prewet Station 3 J28 8 Solvent Waste Station 3 65

67 J29 9 Sample Inlet Station 3 J30 7 Sample Waste Station 3 J31 5 Methanol Rinse Station 3 J32 6 Hexane Rinse Station 3 J33 0 Elution Valve Station 3 J35 None Exhaust Fan J45 -- Top Comm. Port J46 -- Comm. Port Jumper (Closed) J47 -- Bottom Comm. Port J48 None Nitrogen Supply Valve Table 2: Pinout Unused Jumper Connected-To Jumper Connected-To J7 Not Used (Open) J39 Not Used (Open) J25 Not Used (Open) J40 Not Used (Open) J34 Not Used (Open) J41 Not Used (Open) J36 Not Used (Open) J42 Not Used (Open) J37 Not Used (Open) J43 Not Used (Open) J38 Not Used (Open) J44 Not Used (Open) 66

68 7.8. Exhaust Fan Installation Procedure ( ) WORK INSTRUCTION: Rev A 02/25/2016 PURPOSE: The purpose of this procedure is to provide instruction for the replacement of the Exhaust Fan on the SPE-DEX TOOLS: - ¼ Inch Nut Driver/ Socket Wrench - 5/16 Inch Open Wrench PARTS REQUIRED: Fan Assembly PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, drain the solvent from the internal lines and disconnect all external lines going into the instrument. 1. Move the instrument to a location where both the rear and the front of the instrument are easily accessed. 2. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the (11) screws from the rear cover of the instrument. 3. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in and remove the Exhaust Fan wire from the Main Circuit Board. 67

69 4. With the cover removed, use a 5/16 inch wrench to remove the nut in each of the four corners of the fan. 5. Install the Replacement Fan into the appropriate position. Ensure that the labeled side faces the exterior of the instrument and that the electrical wires extend upwards (as in the above picture). NOTE: If the fan is installed incorrectly, it will pull air into the chassis. 6. Re-install the cover onto the chassis being sure to connect the Exhaust Fan to the Main Circuit Board. 7. End of procedure. 68

70 7.9. Pressure Regulator Installation Procedure ( ) WORK INSTRUCTION: Rev A 06/06/2016 PURPOSE: The purpose of this procedure is to provide instruction for the replacement of the Pressure Regulator within a SPE-DEX TOOLS: - ¼ Inch Nut Driver/ Socket Wrench - 3/8 Inch Nut Driver/ Socket PARTS REQUIRED: Pressure Regulator Service Kit Contains: (1) Pressure Regulator with Relief Valve PROCEDURE: NOTE: It is recommended that proper personal protective equipment (PPE) be worn at all times during this procedure. The exact type (lab coat, safety glasses, etc.) is up to the safety policies of the individual laboratory. NOTE: Prior to beginning, drain the solvent from the internal lines and disconnect all external lines going into the instrument. 1. Using a ¼ inch nut driver or a medium flat blade screwdriver, remove the screws that hold the rear cover on. 2. Remove the rear cover from the instrument by sliding the top outwards and separating the cover from the chassis until there is sufficient space to reach in and remove the Exhaust Fan wire from the Main Circuit Board. 69

71 3. On the Pressure Regulator Assembly, locate and remove the (2) 3/8 Inch nuts. There is one on the front-right of the assembly and one on the rear-left of the assembly. Both are located on top of the installed Chassis Standoffs. 4. Remove all (4) of the black nitrogen tubes from the Regulator Assembly. 5. Reattach the tubing to the new Pressure Regulator. 6. Using the (2) 3/8 Inch nuts, attach the Pressure Regulator to the Chassis. 7. Reattach the cover ensuring that the Fan is plugged into the Circuit Board. 8. End of procedure. 70