SPE-DEX 4790 Solid Phase Extraction System

Transcription

1 SPE-DEX 4790 Solid Phase Extraction System Service Manual

2 16 Northwestern Drive, Salem, NH Telephone: (603) Toll-Free: (800) USA Only Website: Copyright 2015 by Horizon Technology, Inc. All rights reserved. Horizon Technology, Inc. reserves the right to change the information in this document without notice. No part of this work may be processed, reproduced, or transmitted in any form or by any means, electronic or mechanical, including photocopying and recording, or by any information storage or retrieval system, except as may be expressly permitted in writing by Horizon Technology, Inc. SPE-DEX is a trademark of Horizon Technology, Inc. All other product names herein are used for identification purposes only and are recognized as properties (including trademarks, registered trademarks, and referenced copyrighted materials) of their respective holders. P/N: REV A (August 2015) 1

3 Contents Preface... 4 Manual Audience and Intent... 4 Technical Support Nomenclature Recommended System Maintenance Daily Maintenance Weekly Maintenance Troubleshooting Preventative Maint. Kits/ Miscellaneous Accessories Preventative Maintenance Procedures Pre-Maintenance Inspection Preventative Maintenance: Module Replacing the Check Valve Replacing the Thermistors Replacing the Bottle Holder O-Ring Replacing the Rinse Needle Replacing the Collection Adapter Screws Replacing the Modified Water Plug Replacing the Module Feet Replacing the Solvent and Water Waste Lines Preventative Maintenance: System Check Valve Tool Thermistor Height Adjustment Tool Hydrophilic Solution Water-Waste Manifold Vacuum Line Kit Post-Maintenance Inspection Service Kits and Parts List Service Replacement Procedures Replacing the Bottle Holder (PN ) Replacing the Water Inlet Valve (PN )

4 7.3. Replacing the Downtube Assembly (PN ) Replacing the Sample Collect Valve ( ) Replacing the Solvent- or Sample-Waste Valve ( ) Replacing a Prewet or Rinse Valve ( ) Replacing the Water Inlet Valve Actuator ( ) Replacing the Collection Adapter ( ) Replacing the MAC Valve ( ) Replacing the Exhaust Fan (PN ) Replacing the Spring Motor (PN ) Replacing the IR Photointerruptor (PN ) Replacing the SPE-DEX 4790 Keypad (PN CE) Replacing the Control Circuit Board (PN CE) Schematics Nitrogen and Solvent Delivery System Waste and Vacuum System Plumbing Schematics MAC Valve and Water Inlet Valve Actuator Prewet and Rinse Plumbing Vacuum Plumbing Electrical Systems Ribbon Cable Control Circuit Board Valve Indicators Operation Code Prewet Cycle Sample Processing Cycle Wash Cycle Air Dry Cycle Rinse Cycle Available Service Documents

5 Preface Manual Audience and Intent This manual, which is intended for all service technicians with Horizon Technology product service training, provides the information needed to perform maintenance on the SPE-DEX The information may contain typographical errors or technical inaccuracies and is subject to change without notice. Modifications or enhancements may also be made to the product at any time. For the most current information, consult Horizon Technical Support. Product Safety The SPE-DEX 4790 is designed with operator safety in mind. However, the product use is at the discretion and risk of the operator or laboratory supervisor/manager. Do not attempt to disassemble or repair the unit before reviewing this guide. It is recommended that the SPE-DEX 4790 only be serviced by trained service technicians and with factory approved components. Electro-Static Discharge To reduce the risk of electrical shock, an Electro-Static discharge mat should be used when troubleshooting or repairing the SPE-DEX Personal Protective Equipment Due to the nature of use for the SPE-DEX 4790, it is recommended that proper Personal Protective Equipment (PPE) be used when servicing it. This should include safety glasses and gloves, but may include additional PPE based on your safety requirements. Technical Support Visit the Horizon website for technical information in addition to that provided in this guide: If you have questions about the SPE-DEX 4790 solid phase extraction system that are not fully addressed in this guide or our website, please contact the Horizon Technology Technical Customer Support Center: Phone: (603) Fax: (603) Customer Support Center hours are Monday through Friday, 8:00 a.m. to 5:00 p.m. EST. The Center provides expert technical support including troubleshooting, repair instructions, service and installation scheduling, and replacement part information. 4

6 Notes: 5

7 1. Nomenclature Throughout this document, the following terms will be used which require definition: Module: A single SPE-DEX 4790 position capable of running a single water sample and collecting an extract. Controller: The control interface for the SPE-DEX This controller may be either the stand-alone 4700 Controller or a PC-connected Envision Controller. Both are capable of controlling up to eight Modules. System: A collection of up to eight (8) SPE-DEX 4790 Modules connected to a single controller and waste system. Preventative Maintenance (PM): A PM is preformed yearly and is designed to maintain a full System and through the measurement of various conditions and the replacement of commonly wearable parts. 6

8 2. Recommended System Maintenance 2.1. Daily Maintenance On a daily basis when the instrument is in routine operation: Clean up any spills immediately using a soft, dry cloth. Follow that by wiping the area with a cloth made damp with a mild nonabrasive general purpose laboratory cleaning solution. Do not spray a cleaning solution directly onto the instrument. Flush the Water-Waste, Solvent-Waste, and Elution vacuum pathways using warm water. Clean accessory components like Disk Holders, Cap Adapters, and Collection Vessels using a mild nonabrasive general purpose laboratory cleaning solution. Follow this cleaning with a rinse of the final elution solvent for best results. Ensure that all gaskets are in place and in good condition. Verify that all Plastic-Coated Solvent Bottles are free from defects. If needed, replace them prior to pressurizing Weekly Maintenance At the end of the week when the instrument is in routine operation: Check the gas supply and tubing to ensure that all of the fittings are properly seated and that the tube is not damaged in any way. Check the solvent tubing and ensure that all fittings are properly seated and there is no damage. Check the exhaust ducting, if needed, and ensure that the attachments at both ends are secure and free of obstruction. Verify that the ducting is not crimped or crushed by other instruments or heavy objects. Flush the Water-Waste, Solvent-Waste, and Elution vacuum pathways using acetone. Follow this with warm water. 7

9 3. Troubleshooting SYMPTOM PROBABLE CAUSE SOLUTION Data Cable not attached. Module not displayed on the Envision Operation Page. Prewet solvent does not dispense. Fault 80: Extractor Fault: Prewet Solvent Delivery Fault: Check Extractor Bad Data Cable. Unfamiliar method. Low/No pressure. Low/No solvent in connected bottle. Solvent Sip Tube not attached to cap. Clogged Solvent Filter Kinked Solvent Delivery line Valve not actuating. Solvent does not reach the top thermistor in under 1 min. Ensure that the Data Cable is properly seated on both the Module s and the Envision s Data Port. Connect the Data Cable to a different port on the Envision and see if communication can be established. Replace Cable if necessary. Connect the Data Cable to a different extractor Confirm method steps to ensure that the prewet is scheduled. Confirm that the method has been loaded onto the extractor in use. Verify that nitrogen source is set correctly. Verify that the Solvent Bottle Pressure is set correctly. Verify that all nitrogen lines are free from kinks. Verify that the bottle cap is tight and that both gaskets are present and undamaged. Verify that enough solvent is contained within the solvent bottle to cover the bottom of the solvent filter. Ensure that the sip tube is tightened into the cap. Cut or pull Solvent Filter off and verify that solvent is able to be dispensed. Replace if needed. Inspect all Solvent Delivery lines, replace if needed. Ensure that the valve pins are properly seated within the Molex Connector. Other valves not actuating; replace the fuse on Control Circuit Board Refer to "Prewet solvent does not dispense." 8

10 SYMPTOM PROBABLE CAUSE SOLUTION Prewet solvent overflows. Incorrectly set Thermistors Use the Thermistor Height Tool to ensure the recommended height is set. Pressure is set too high. Verify the nitrogen source pressure is set correctly. Verify that the Solvent Bottle Pressure is set correctly. Bad Thermistors. Replace the Top Thermistor. Prewet solvent is not drained. Insufficient vacuum. Verify that the vacuum source is set correctly. Verify that the Water-Waste Carboy Manifold is correctly seated. Close the Water-Waste Carboy Drain. Verify the Solvent Waste vacuum Prewet solvent is drained immediately. Extractor continually drains after first Prewet. Prewet solvent drips from the Downtube. Solvent-Waste valve is not opening. Waste valve is stuck open. Check Valve is stuck open. Incorrectly set Thermistor. Thermistors heights reversed Bad lower Thermistor. Prewet valve stuck open is set correctly. Write a short method with only 1 prewet in it and run. Replace valve if no actuation is seen. Place DI Water into Disk Holder attached to a module. Watch the waste containers and identify which one the water is pulled into. Use the Check Valve Tool to cycle the Check Valve. Place DI Water into a Disk Holder attached to a Module. Replace Check Valve if necessary. Use the Thermistor Height Tool to ensure the recommended height. Verify that the higher of the two Thermistors is connected to J6 on the Control Circuit Board. Replace Thermistor. Increase the Solvent Bottle Pressure to 17 psi and run a method that fires only the affected valve. Replace the valve if necessary. Sample does not dispense. Low/No pressure. Verify that the nitrogen source is set correctly. Verify that the Extractor Pressure is set correctly. Water Inlet Valve Actuator is Increase Extractor Pressure to 9

11 SYMPTOM PROBABLE CAUSE SOLUTION stuck closed psi and run the Sample Drain Method. Actuate the Water Inlet Valve manually. Verify that the MAC valve is firing by disconnecting the nitrogen line on the top port of the Actuator and running the Sample Drain method (this is line A at the MAC valve). If the line does not pulse nitrogen, replace the actuator. Water Inlet valve needs to be treated (shaking the extractor Treat the valve using Hydrophilic Solution. allows sample to flow through the valve for a limited time). Sample overflows. Air leak. Ensure that all cap adapters contain an appropriate gasket which is undamaged. Verify that the sample bottle being used does not have defects on the threads or the rim. Replace the Bottle Holder O- Ring. Replace the Cap Adapter. Sample flows slowly or not at all. Insufficient vacuum. Verify that the vacuum source is set correctly Verify that the Water-Waste Carboy Manifold is correctly seated. Close the Water-Waste Carboy Drain. Insufficient prefilters used. Use additional prefilters to increase the flow rate through the SPE Disk. Use the EZ Flow Holder for more prefiltering surface area. Disk Holder Support Screen is off-center. Water-Waste valve is not opening. Water Waste line is kinked 10 Open the disk holder and verify that the imprint of the Support Screen on the bottom of the SPE disk is off-center. With no disk in the disk holder, run DI Water using the Sample Drain method to confirm that the valve needs replacement. Verify that all waste lines are free from kinks.

12 SYMPTOM PROBABLE CAUSE SOLUTION Slow sample flow rate through SPE disk. System advances to Air Dry without having processed the entire sample. System does not advance to Air Dry. Bad Thermistor. Thermistors remained covered. 11 Verify Thermistor heights using the Thermistor Height Setting Tool. Investigate other prefiltering options. Verify Thermistor heights using the Thermistor Height Setting Tool. Ensure that Thermistors are connected to the Control Circuit Board correctly. Replace if necessary. Raise the Thermistor Clip so it is not touching anything within the Disk Holder. Upgrade the chip. System times out after 60 minutes. Old version of Extractor Software Code. Water Inlet Valve (WIV) drips. Damaged Water Inlet Valve. Look down into the WIV and confirm that there are scratches on the Spool. Replace WIV. Incorrectly set tension on WIV. Look down into the WIV and run the Sample Drain method. WIV should open in 1 second. Opening faster indicates the tension may be insufficient. Fluid leaking from Rinse Rod. Rinse Valve is stuck open. Increase the Solvent Bottle Pressure to 17 psi and run a method that fires only the affected valve. If doing this does not work, replace the valve. Rinses not dispensed. Unfamiliar method. Confirm method steps Confirm that the method has been loaded onto the module in use. Low/No pressure. Low/No solvent in connected bottle Verify that nitrogen source is set correctly. Verify that the Solvent Bottle Pressure is set correctly. Verify that all nitrogen lines are free from kinks. Verify that the bottle cap is tight and that both gaskets are present and undamaged. Verify that enough solvent is contained within the solvent bottle to cover the bottom of the solvent filter.

13 SYMPTOM PROBABLE CAUSE SOLUTION Solvent Sip Tube not attached to cap. Ensure that the Sip Tube is tightened into the cap. Clogged Solvent Filter Cut or pull Solvent Filter off and verify that solvent is able to be dispensed. Kinked delivery line. Inspect all lines. Rinse Rod is blocked. Pull the Rinse Rod out of the Bottle Holder and examine by holding it up to the light. Sonicate or replace if necessary. Valve not actuating. Ensure that the valve pins are properly seated within the Molex Connector. Other valves not actuating; replace the fuse on Control Circuit Board. Rinses and Washes are 2 seconds IR Sensor had failed. Replace the IR sensor. even though the 8-Second Magnet is used. Fluid drips into the Collection Vessel. Check Valve needs cleaning. Use Check Valve Tool to free Check Valve Fill the Disk Holder with warm water and press and hold Abort then press Purge. Check Valve needs to be Replace Check Valve. replaced. Low/No Elution volume. Unfamiliar method. Confirm method steps. Confirm that the method has been loaded onto the extractor in use. Check Valve is stuck. Rinse solvent was dispensed, but not pulled into the Disk Holder. Use Check Valve Tool to free Check Valve. Collection Vessel improperly attached. Insufficient vacuum. 12 Attach Collection Vessel to Collection Adapter using an upwards-twisting motion to ensure a good seal. Verify that the vacuum source is set correctly. Verify that the Water-Waste Carboy Manifold is correctly seated. Close the Water-Waste Carboy Drain. Verify the Solvent Waste vacuum is set correctly.

14 SYMPTOM PROBABLE CAUSE SOLUTION Waste valve is stuck open. With the extractor idle, fill a disk holder with water and verify that the fluid level does not decrease. If it does, examine the Waste vessels to determine which valve is stuck open and replace that valve. Keypad lights have dimmed or gone out. LEDs in Keypad are burned out. Replace Keypad. 13

15 4. Preventative Maint. Kits/ Miscellaneous Accessories This is a list of the standard preventative maintenance kits & accessories that can be purchased from Horizon Technology. To ease the process of performing a yearly preventative maintenance (PM), the parts required are bundled into two Kits. A Module Kit allows for the maintenance of a single SPE-DEX 4790 module; Service Engineers will need to have one kit for each extractor Module. The System Kit allows for the maintenance of the external components and accessories of the System; only one kit is needed per waste container set. If a user has a single controller, but multiple waste containers, an additional kit will be needed. Some of the parts contained within the PM Kits are available by themselves if needed, however many of the individual parts are not. Part # Description Check Valve Tool Thermistor Height Adjustment Tool Hydrophilic Solution 14

16 Check Valve Thermistor (set of two) Bottle Holder O-Ring Rinse Needle (pack of two) Thermistor Clip Solvent-Waste Manifold Water-Waste Manifold 15

17 EX-THRU EX-END Solvent Manifold - Through Block Includes: (1) Solvent Manifold: Through Block (18) Manifold Plugs (18) Solvent Manifold Delivery Lines (9) Manifold-to-Manifold Delivery Lines Solvent Manifold - End Block Includes: (1) Solvent Manifold: End Block (18) Manifold Plugs (18) Solvent Manifold Delivery Lines (9) Manifold-to-Manifold Delivery Lines Solvent Manifold Delivery Lines Solvent Bottle Delivery Lines (includes eight solvent lines and one nitrogen line) Solvent Bottle Tops (pack of eight) 16

18 Solvent Stone (includes ferrule) Manifold-to-Manifold Delivery Line (single line only; nine needed for complete set) Solvent Manifold: End Block Solvent Manifold: Through Block 17

19 SPE-DEX 4790 System PM Kit This kit is designed to service the external components which are attached to a single waste collection system or controller. o Check Valve Tool o Thermistor Height Adjustment Tool o Hydrophilic Solution o Water-Waste Manifold o Vacuum Line Kit SPE-DEX 4790 Module PM Kit This kit is designed to service a single SPE-DEX 4790 module. For systems containing multiple modules, the same number of kits will be ordered. 18

20 o Check Valve o Check Valve Screws (3 Included) o Thermistors (set of two) o Bottle Holder O-Ring o Solvent and Water Waste Lines o Rinse Needle o Collection Adapter Screws (three Included) o Modified Water Plug 19

21 o Module Feet (six included) Vacuum Gauge Kit This tool allows for the measurement and analysis of the condition of the subsystems for a SPE-DEX

22 5. Preventative Maintenance Procedures 5.1. Pre-Maintenance Inspection Prior to starting any work, it is recommended that the Service Engineer evaluate the System using the Instrument Repair Report, available upon request from Horizon Technology. This section will explain the Pre- Inspection Testing section on this form and will require that the Service Engineer is able to run the System. Note: The following procedures will run the SPE-DEX System through a complete run where solvent is used. It is recommended that the Service Engineer use Safety Glasses and Gloves when using the equipment. System Serial Numbers 1. Record the serial numbers for all instruments. Their serial numbers can be used to log the parts and service work which has been completed on that unit for future reference. Pre-Maintenance Inspection Testing 2. Turn on the computer which is used to run the System. 3. Turn the Envision s power on. 4. Turn on the Nitrogen Source and regulate it to between 60 and 80 psi (4.1 to 5.5 bar). 5. Turn on the Vacuum Source and regulate it to between -23 and -26 Hg (-779 to -880 mbar). 6. Record the vacuum and nitrogen readings in the table. 7. Using the computer, connect to the Envision and ensure that all Modules connected are displayed. 8. Within the Controller, write a method similar to the following: Note: Solvent names have not been included as the solvent used should be the one that the end-user already has connected to the units. Step Soak Time Dry Time Prewet 1 0 sec 30 sec Prewet 2 0 sec 30 sec Prewet 3 0 sec 30 sec Prewet 4 0 sec 30 sec Prewet 5 0 sec 30 sec Sample Process Air Dry 60 sec Rinse 1 0 sec 30 sec Rinse 2 0 sec 30 sec Rinse 3 0 sec 30 sec 9. Load the method to all Modules. 10. Prepare all Modules by loading an empty Disk Holder, a Sample Container with ml of DI Water in it, and an empty Collection Vessel. 21

23 11. Starting with Module 1, run all the Modules one at a time and record their responses in the table in the Instrument Repair Report. a. For Prewet valves, ensure that the solvent dispenses until the level reaches the top Thermistor and then stops. Solvent should be pulled to the Solvent-Waste Container. b. When the Sample Loading step occurs, ensure that the Water-Inlet-Valve (WIV) opens and that the flow of the water into the Disk Holder is steady. Water should be pulled through the instrument and deposited into the Water-Waste Container. c. Each Rinse step should deliver a steady stream of solvent to the center of the sample container. It is acceptable for the rinse stream to be off-center by the radius of a U.S. Quarter coin or a Euro- Zone 2 coin. d. The Solvent-Waste valve should activate during the prewet steps to remove the solvent. e. The Water-Waste valve should activate during the Sample Processing step to remove the water. f. The Elution valve should activate during the Rinse steps to pull the solvent into the Collection Vessel. 12. Using the Vacuum Test apparatus and the T-Fitting, measure the vacuum as follows for each Module: a. Turn off the nitrogen and de-pressurize the Solvent Bottles. This will prevent solvent from moving during the next tests. b. Fill a beaker or other cup partially with tap water. c. Slide the Thermistor Clip, with the Thermistors attached, off of the Downtube as shown in the picture. d. Create a method within the Envision system as follows: Step Soak Time Dry Time Prewet 1 0 sec 60 sec Sample Process Air Dry 60 sec Rinse 1 0 sec 60 sec e. Load the method onto all Modules. f. With the Vacuum Test gauge installed into the Module Platform, press Start g. Using the beaker or cup of water, after 15 seconds, put the Thermistors into the water. This will trick the SPE-DEX into moving from dispensing solvent to draining the solvent, activating the Solvent-Waste valve. h. Measure and record the Solvent-Waste vacuum. The nominal value for this reading is the value that can be read on the Solvent-Waste Manifold (typically -15 Hg ± 2 Hg). i. When the Sample Process begins, wait 10 seconds, and then place the Thermistor Clip into the beaker or cup of water. This will trick the thermistors and activate the Water-Waste pathway. j. Measure and record the Water-Waste vacuum. The nominal value for this reading is the value that can be read on the Water-Waste Manifold (typically -24 Hg ± 2 Hg). k. After the rinse has occurred (no solvent will spray out) measure and record the Elution vacuum. The nominal value for this reading is -6 to -9 Hg. 22

24 5.2. Preventative Maintenance: Module For each Module which a PM is to be performed on, a single SPE-DEX 4790 Module PM Kit (PN ) will be required. The following sections are meant to help ensure that the parts included are installed correctly Replacing the Check Valve 1. Remove the three screws which hold the Check Valve into the Collection Adapter and discard them. 2. Using pliers, pull the Check Valve out of the Collection Adapter and discard. 3. Install the new Check Valve into the Collection Adapter ensure that the vacuum port is located to the left side of the Module as shown in the picture. 4. Use the new screws provided to install the Check Valve. Vacuum Port Note: It is critical that the vacuum port be correctly located for the elution process to function. Note: The Collection Adapter is made of soft plastic and can have the screw holes stripped if the screws are tightened too forcefully. Care should be taken Replacing the Thermistors 1. Remove the five (5) screws on the back of the Module using a 1/4 nut driver or screwdriver. 2. Remove the Module cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 3. Slide the Thermistor Clip off of the Down tube leaving the Thermistors attached. 4. Follow the wires for one of the Thermistors to the board and disconnect it. 5. Pull the wires through the Module and disconnect the Thermistor from the Thermistor Clip. 6. Discard the Thermistor. 7. Using the new Thermistor, route the wires back through the chassis and attach the wires to the Main Circuit Board. Top Thermistor When Facing Extractor Thermistor Height Main Circuit Board Connector Right Side Top J6 Left Side Bottom J7 Bottom Thermistor 23

25 8. Attach the Thermistor to the Thermistor Clip. 9. Do the same for the other Thermistor. 10. Set the height of the Thermistors using the Thermistor Height Adjustment Tool (see the appropriate section below). 11. Replace the cover and screws on the Module. Note: The connection to the Main Circuit Board dictates the height of the Thermistor. If improperly set, the Prewet and Sample Processing steps may generate errors. It is recommended that the Service Engineer use a plastic zip tie or other mechanism to indicate either the side or the height so that, if the Thermistor is knocked out of the Thermistor Clip, they can be reset with confidence Replacing the Bottle Holder O-Ring The Bottle Holder O-Ring is responsible for maintaining a seal with the Cap Adapter. If this gasket is worn too far, then air leaks may develop and sample overflows will occur. 1. Using a pick or other similar tool, hook the O-Ring contained within the Bottle Holder and pull the O-Ring out. 2. Replace the O-Ring with the new one by hand. Note: The Bottle Holder is made of soft plastic and can be scored easily if care is not taken in the removal process. It is recommended that the O-Ring be removed by puncturing it slightly with a pick and pulling gently Replacing the Rinse Needle 1. Using long-nose pliers, remove the Rinse Needle by gripping the needle and pulling straight up. 2. Install the new Rinse Needle into the hole in the Bottle Holder. Push straight down until the Needle hits the bottom of the hole. Note: The Rinse Needle has a single hole in the side at the bottom. It is important that this hole be positioned facing the rear of the instrument or Rinse and Wash solvents will not be dispensed. 24

26 Replacing the Collection Adapter Screws 1. Unscrew the three screws which hold the Collection Adapter to the Module Platform. 2. Use the screws provided to reattach the Collection Adapter to the Platform. Note: The Collection Adapter is made of soft plastic and can have the screw holes stripped if the screws are tightened too forcefully. Care should be taken Replacing the Modified Water Plug The Modified Water Plug is a Leur Fitting that has had a specific size hole put in it so that the vacuum contained within the Water-Waste lines are relieved after a run. Doing this removes the load from the valves and prolongs their lifetime. With time, this hole can become occluded and the flow rate of a sample will increase and may lead to poor results. 1. Remove the five (5) screws on the back of the Module using a ¼ nut driver or screwdriver. 2. Remove the Module cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 3. At the top of the Module, there is a T-fitting that ends with a plugged line as shown in the picture. 4. Unscrew the Modified Plug and replace with the one provided in the kit. 25

27 5. Replace the cover and screws on the Module Replacing the Module Feet 1. Remove the exhaust tubing from the rear of the instrument. 2. Lay the instrument on its side, being careful not to kink any of the external solvent tubing. 3. Remove the old feet using either a pair of pliers to pull them off or by scraping the feet off using a knife. 4. Install the new feet as shown in the picture below: Replacing the Solvent and Water Waste Lines The waste lines can have solid material which builds up over time. The replacement of these lines yearly will maintain the proper flow rate of fluid through them. 1. On the rear of the instrument, remove and discard the Waste-Waste and the Solvent-Waste lines. 2. Install the new Solvent-Waste line (the one with the black collar on it) onto the Solvent-Waste port. 3. Install the new Water-Waste line (the one with no collar on it) onto the Water-Waste port. 4. Trim both lines to the appropriate length. 5. Install barb fittings into both of the lines. 6. Connect the Solvent-Waste line to an open port on the Solvent-Waste Manifold. 7. Connect the Water-Waste line to an open port on the Water-Waste Manifold. 26

28 5.3. Preventative Maintenance: System A specific point to the System PM Kit is to replace those tools which are vital to the running of the System every day. The following sections will detail the replacement of those parts which are mutual to all Modules Check Valve Tool This tool allows users to ensure that the Check Valve is free. This tool is often misplaced by the end users and is included due to its importance. 1. Push down on the Platform on the front of the 4790 to allow more room between the Downtube and the Platform. 2. While holding the Platform down, place the long, thin end of the Check Valve Tool into the hole. 3. Tap the top of the Check Valve Tool gently. The Tool should bounce freely up and down with no resistance. Note: The Check Valve contains a small ceramic ball which may be damaged if a substitute is made for the Check Valve Tool. Materials which are harder than the ceramic may chip or gouge the ball Thermistor Height Adjustment Tool The height of the Thermistors determines the amount of solvent which is dispensed during a Prewet step as well as indicates to the instrument when sample matrix is no longer present in the Disk Holder. The optimum height of the Thermistors are easily set using this tool. 1. Remove the Thermistor Clip from the Downtube. 2. Using the Side of the Tool, adjust the height of the Thermistors relative to each other and the Thermistor Clip. 3. Slide the Thermistor Clip back onto the Downtube. 4. Using the top of the Adjustment Tool, position the Thermistors so that the bottom Thermistor just touches Tool. 27

29 Hydrophilic Solution Since the Water Inlet Valves are made of hydrophobic material, they are treated with a Hydrophilic Solution to ensure the rapid and complete delivery of the sample from the Sample Container to the Disk Holder. Without this treatment, the sample may not completely flow into the Disk Holder. 1. Remove the five (5) screws on the back of the Module using a ¼ nut driver or screwdriver. 2. Remove the Module cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 3. Slide the Thermistor Clip off of the Downtube. 4. Place one of the included vials on the Downtube. 5. Within the Module, locate the Water Inlet Valve Actuator and open the valve by turning the shaft that protrudes from the rear of it using a wrench. 6. Pour the Hydrophilic Solution into the Bottle Holder until it comes up to just below the Bottle Holder Gasket. 7. Open and Close the Water Inlet Valve manually two or three times. 8. Let the Module soak for at least two hours, overnight is best. 9. Drain the Hydrophilic Solution back into the bottle it came in to be used again later. 10. Reinstall the cover and screws on the Module Water-Waste Manifold As a system ages, the Stopper on the Water-Waste Manifold wears. In addition, the elbow fittings may become occluded with solid material. Both of these causes may lead to a lower vacuum rating and slower flow rate. 1. Disconnect all lines from the old Water-Waste Manifold and discard the Manifold. 2. Install all lines onto the new Manifold making sure that the lines attached to the Vacuum Pump and the Solvent-Waste Container are on top. 28

30 Note: It is critically important that vacuum supply lines are located above all lines which carry fluid to prevent the aspiration of fluid into the vacuum pump Vacuum Line Kit Through constant use, the Vacuum Lines may become occluded with solid material leading to lower vacuum and slower flow rates. 1. Disconnect the T-Fitting from the top of the Water-Waste Manifold. 2. Remove the Vacuum Line from the rear of the Solvent-Waste Manifold. 3. Remove the Vacuum Line from the Vacuum Pump. 4. Discard all Lines and the T-Fitting. 5. Attach the new T-Fitting to the top of the Water-Waste Manifold. 6. Attach one end of the larger diameter Vacuum Line to the Vacuum Pump. 7. Attach the other end to one port of the T-Fitting. 8. Attach one end of the smaller diameter Vacuum Line to the rear of the Solvent-Waste Manifold. 9. Attach the other end to one port on the T-Fitting. 29

31 5.4. Post-Maintenance Inspection After the completion of the PM, it is recommended that the Service Engineer re-evaluate the System using the Instrument Repair Report. This section will explain the Post-Maintenance Inspection Testing section on this form and will require that the Service Engineer is able to run the System. Note: The following procedures will run the SPE-DEX System through a complete run where solvent is used. It is recommended that the Service Engineer use Safety Glasses and Gloves when using the equipment. 1. Turn on the computer which is used to run the System. 2. Turn the Envision s power on. 3. Turn on the Nitrogen Source and regulate it to between 60 and 80 psi (4.1 to 5.5 bar). 4. Turn on the Vacuum Source and regulate it to between -23 and -26 Hg (-779 to -880 mbar). 5. Record the vacuum and nitrogen readings in the table. 6. Using the computer, connect to the Envision and ensure that all Modules connected are displayed. 7. Within the Controller, write a method similar to the following: Note: Solvent names have not been included as the solvent used should be the one that the end-user already has connected to the units. Step Soak Time Dry Time Prewet 1 0 sec 30 sec Prewet 2 0 sec 30 sec Prewet 3 0 sec 30 sec Prewet 4 0 sec 30 sec Prewet 5 0 sec 30 sec Sample Process Air Dry 60 sec Rinse 1 0 sec 30 sec Rinse 2 0 sec 30 sec Rinse 3 0 sec 30 sec 8. Load the method to all Modules. 9. Prepare all Modules by loading an empty Disk Holder, a Sample Container with ml of DI Water in it, and an empty Collection Vessel. 10. Starting with Module 1, run all the Modules one at a time and record their responses in the table in the Instrument Repair Report. a. For Prewet valves, ensure that the solvent dispenses until the level reaches the top Thermistor and then stops. Solvent should be pulled to the Solvent-Waste Container. b. When the Sample Loading step occurs, ensure that the Water-Inlet-Valve (WIV) opens and that the flow of the water into the Disk Holder is steady. Water should be pulled through the instrument and deposited into the Water-Waste Container. c. Each Rinse step should deliver a steady stream of solvent to the center of the sample container. It is acceptable for the rinse stream to be off-center by the radius of a U.S. Quarter coin or a Euro- Zone 2 coin. d. The Solvent-Waste valve should activate during the prewet steps to remove the solvent. 30

32 e. The Water-Waste valve should activate during the Sample Processing step to remove the water. f. The Elution valve should activate during the Rinse steps to pull the solvent into the Collection Vessel. 11. Using the Vacuum Test apparatus and the T-Fitting, measure the vacuum as follows for each Module: a. Turn off the nitrogen and de-pressurize the Solvent Bottles. This will prevent solvent from moving during the next tests. b. Fill a beaker or other cup partially with tap water. c. Slide the Thermistor Clip, with the Thermistors attached, off of the Downtube as shown in the picture. d. Create a method within the Envision system as follows: Step Soak Time Dry Time Prewet 1 0 sec 60 sec Sample Process Air Dry 60 sec Rinse 1 0 sec 60 sec e. Load the method onto all Modules. f. With the Vacuum Test gauge installed into the Module Platform, press Start g. Using the beaker or cup of water, after 15 seconds, put the Thermistors into the water. This will trick the SPE-DEX into moving from dispensing solvent to draining the solvent, activating the Solvent-Waste valve. h. Measure and record the Solvent-Waste vacuum. The nominal value for this reading is the value that can be read on the Solvent-Waste Manifold (typically -15 Hg ± 2 Hg). i. When the Sample Process begins, wait 10 seconds, and then place the Thermistor Clip into the beaker or cup of water. This will trick the thermistors and activate the Water-Waste pathway. j. Measure and record the Water-Waste vacuum. The nominal value for this reading is the value that can be read on the Water-Waste Manifold (typically -24 Hg ± 2 Hg). k. After the rinse has occurred (no solvent will spray out) measure and record the Elution vacuum. The nominal value for this reading is -6 to -9 Hg. 31

33 6. Service Kits and Parts List This is a list of the service kits that can be purchased from Horizon Technology. Part # Description Bottle Holder (includes Bottle Holder O-Ring and Rinse Needle) Water Inlet Valve Downtube Assembly Sample Collect Valve Solvent-Waste Valve or Sample-Waste Valve Prewet and Rinse Valve (24 V) 32

34 Water Inlet Valve Actuator Collection Adapter MAC Valve (includes connectors and barbs) Exhaust Fan (includes Molex connectors) Spring Motor (includes bolt and nut) IR Photointerrupter 33

35 CE SPE-DEX 4790 Key Pad CE Control Circuit Board External Nitrogen Regulator 34

36 7. Service Replacement Procedures Due to the nature of use for the SPE-DEX 4790, it is recommended that proper Personal Protective Equipment (PPE) be used when servicing the System. This is to include safety glasses and gloves, but may include additional PPE based on your safety requirements Replacing the Bottle Holder (PN ) PURPOSE: This work instruction will replace the Bottle Holder on a SPE-DEX 4790 Module. TOOLS: - None PARTS REQUIRED: Bottle Holder CAUTIONS: Prior to beginning, the Module being worked on should have all rinse solvents drained and the lines flushed to prevent spills. Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. PROCEDURE: 1. Pull up on the Bottle Holder to detach it from the Water Inlet Valve. 2. Unscrew the (3) Rinse Lines from the rear of the Bottle Holder. 3. Install the (3) Rinse Lines into the new Bottle Holder 4. Push the Bottle Holder onto the Water Inlet Valve making sure to align the front post. 5. Procedure complete. 35

37 7.2. Replacing the Water Inlet Valve (PN ) PURPOSE: This work instruction will replace the Water Inlet Valve on a SPE-DEX 4790 Module. TOOLS: - 5/32 inch Hex Wrench - Phillips-head Screwdriver PARTS REQUIRED: Water Inlet Valve CAUTIONS: Prior to beginning, the Module being worked on should have all rinse solvents drained and the lines flushed to prevent spills. Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. PROCEDURE: 1. Pull up on the Bottle Holder to detach it from the Water Inlet Valve. Do not remove the Rinse lines from the Bottle Holder. 2. Remove the (4) Hex screws that hold the Water Inlet Valve and the Downtube Assembly onto the Module. 3. Locate and unscrew the two phillips-head screws on the bottom of the Downtube Assembly. Note: Care should be taken not to kink the Prewet lines that are attached to the Downtube Assembly when unscrewing it. 4. Install the new Water Inlet Valve onto the Downtube Assembly. 5. Attach the Water Inlet Valve to the Module using the (4) Hex screws. 6. Push the Bottle Holder onto the Water Inlet Valve making sure to align the front post. 7. Procedure complete. 36

38 7.3. Replacing the Downtube Assembly (PN ) PURPOSE: This work instruction will replace the Downtube Assembly on a SPE-DEX 4790 Module. TOOLS: - 5/32 inch Hex Wrench - Phillips-head Screwdriver PARTS REQUIRED: Downtube Assembly CAUTIONS: Prior to beginning, the Module being worked on should have all prewet and rinse solvents drained and the lines flushed to prevent spills. Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. PROCEDURE: 1. Pull up on the Bottle Holder to detach it from the Water Inlet Valve. Do not remove the Rinse lines from the Bottle Holder. 2. Remove the (4) Hex screws that hold the Water Inlet Valve and the Downtube Assembly onto the Module. 3. Locate and unscrew the two phillips-head screws on the bottom of the Downtube Assembly. Set the Water Inlet Valve aside for reuse. Note: Care should be taken not to kink the Prewet lines that are attached to the Downtube Assembly when unscrewing it. 4. Unscrew the (5) prewet lines from the rear of the Downtube Assembly. 5. Attach the (5) prewet lines to the new Downtube Assembly. 6. Install the Water Inlet Valve onto the new Downtube Assembly. 7. Attach the Water Inlet Valve to the Module using the (4) Hex screws. 8. Push the Bottle Holder onto the Water Inlet Valve making sure to align the front post. 9. Procedure complete. 37

39 38

40 7.4. Replacing the Sample Collect Valve ( ) PURPOSE: This work instruction will outline the process to replace Sample Collect Valve within a SPE-DEX 4790 Module. TOOLS: - ¼ inch Nut Driver - 5/16 inch Wrench - Molex Pin Extraction Tool ( ) - Small Standard Screwdriver - Small Phillips Screwdriver PARTS REQUIRED: Sample Collect Valve Molex Pin Extraction Tool CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. PROCEDURE: 1. Remove the five (5) screws from the rear of the Module using the ¼ inch Nut Driver. 2. Remove the cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. Sample Collect Valve 3. Locate the mounting bracket. The Sample Collect Valve is located on the rear side of the mounting bracket. 4. Loosen the 5/16 hexagonal nut and the screw, circled in red. 5. Depress the locking mechanism and separate the two halves of the Molex connector. 6. Carefully cut the head off of all the wire ties on the yellow electrical lines. 7. The pin layout for the Molex connector half attached to the valves is shown below. Note that the locking mechanism is shown up. 8. Remove pins 1 and 2 for the Sample Collect Valve. Refer to the pictures below for the proper removal technique. 39

41 Press Here to Unlock Rotate the valve bracket and remove the screws for the valve. 10. Unscrew the vacuum supply from the top and side of the valve. 11. Using the replacement valve supplied in the kit, attach the bottom vacuum line to the port on the valve labeled NC. 12. Attach the side vacuum line to the port labeled COMM. 13. Using the removed screws, attach the replacement valve to the valve bracket. 14. Plug the electrical pins into the Molex connector taking care to orient the pin such that the u-channel aligns with the post inside the Molex connector. The pin will only fit in one direction. 15. Attach both halves of the Molex connector together. 16. Replace the bracket screw, but do not fully tighten. Tighten the 5/16 hexagonal nut while holding the bracket to ensure that the bracket is in place. Tighten the bracket screw. 17. Place the extractor cover on to the extractor, making sure that the front tabs are in place. 18. Starting with the top middle hole, replace the screws, but so not fully tighten until all screws are in place. 40

42 7.5. Replacing the Solvent- or Sample-Waste Valve ( ) PURPOSE: This work instruction will outline the process to replace either the Solvent-Waste Valve or the Sample- Waste Valve within a SPE-DEX 4790 Module. The valves are identical in nature but are connected to the vacuum and electrical supplies differently. TOOLS: - ¼ inch Nut Driver - 5/16 inch Wrench - Molex Pin Extraction Tool ( ) - Small Standard Screwdriver - Small Phillips Screwdriver PARTS REQUIRED: Solvent-Waste / Sample-Waste Valve Molex Pin Extraction Tool CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. PROCEDURE: 19. Remove the five (5) screws from the rear of the Module using the ¼ inch Nut Driver. 20. Remove the cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. Solvent to Waste Valve Water to Waste Valve 21. Locate the mounting bracket and identify the valve which needs to be replaced using the labeled picture. 22. Loosen the 5/16 hexagonal nut and the screw, circled in red. 23. Depress the locking mechanism and separate the two halves of the Molex connector. 24. Carefully cut the head off of all the wire ties on the yellow electrical lines. 25. The pin layout for the Molex connector half attached to the valves is shown below. Note that the locking mechanism is shown up. 41

43 26. Remove the pins for valve that needs to be replaced. For the solvent to waste valve, remove pins 3 and 4. For the water to waste valve, remove pins 5 and 6. Refer to the pictures below for the proper removal technique. Press Here to Unlock Rotate the valve bracket and remove the screws for the appropriate valve. 28. Unscrew the vacuum supply from the top and bottom of the valve. 29. Using the replacement valve supplied in the kit, attach the bottom vacuum line to the port on the valve labeled INLET. 30. Attach the top vacuum line to the port labeled OUTLET. 31. Using the removed screws, attach the replacement valve to the valve bracket. 32. Plug the electrical pins into the Molex connector taking care to orient the pin such that the u-channel aligns with the post inside the Molex connector. The pin will only fit in one direction. 33. Attach both halves of the Molex connector together. 34. Replace the bracket screw, but do not fully tighten. Tighten the 5/16 hexagonal nut while holding the bracket to ensure that the bracket is in place. Tighten the bracket screw. 35. Place the extractor cover on to the extractor, making sure that the front tabs are in place. 36. Starting with the top middle hole, replace the screws, but so not fully tighten until all screws are in place. 42

44 7.6. Replacing a Prewet or Rinse Valve ( ) PURPOSE: This work instruction will replace a Prewet or Rinse Valve within in a SPE-DEX 4790 Module. The valves are identical in nature. TOOLS: - ¼ inch Nut Driver - 5/32 inch Hex Wrench - Molex Pin Extraction Tool ( ) - Needle Nose Pliers PARTS REQUIRED: Prewet and Rinse Valve (24 V) Molex Pin Extraction Tool CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. The nitrogen supply should be turned off and the solvent lines purged of solvents to prevent spills. PROCEDURE: 1. Remove the five (5) screws from the rear of the Module using the ¼ inch Nut Driver. 2. Remove the cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 3. Locate the valve which needs to be replaced on the manifold. Prewet 1 Prewet 2 Prewet 3 Prewet 4 Prewet 5 Rinse 1 Rinse 2 Rinse 3 4. Disconnect the Molex connector. 5. Carefully clip the wire ties which secure the valve wiring together. 6. Follow the wires from the afflicted valve to the Molex connector to determine the wires which will need to be removed from the connector. 7. Using the Molex tool, remove the appropriate wires from the connector. 43

45 8. Using needle-nose pliers, unscrew the afflicted valve from the valve block by turning the collar counter-clockwise. If this is not possible, remove the external solvent delivery lines from the unit and, using the 5/32 inch hex wrench, unscrew the entire valve block from the SPE-DEX This will allow easier access to the afflicted valve. When the valve is removed, be sure that the black gaskets do not remain in the block. 9. Install the replacement gaskets into the recess within the valve block and press the replacement valve into position over them. The direction of the valve does not matter. 10. Tighten the valve collar by turning it clockwise. 11. If removed, reinstall the valve block into the SPE-DEX Plug the electrical pins into the Molex connector taking care to orient the pin such that the u-channel aligns with the post inside the Molex connector. The pin will only fit in one direction. 13. Attach both halves of the Molex connector together. 14. It is recommended that, prior to putting the cover on the system be pressurized and run to verify that no leaks are present. If a leak occurs, a gasket may have seated incorrectly or the valve may not be fully tightened. 15. Place the extractor cover on to the extractor, making sure that the front tabs are in place. 16. Starting with the top middle hole, replace the screws, but so not fully tighten until all screws are in place. 44

46 7.7. Replacing the Water Inlet Valve Actuator ( ) PURPOSE: This work instruction will replace the Water Inlet Valve Actuator in a SPE-DEX 4790 Module. TOOLS: - ¼ inch Nut Driver - Phillips Screwdriver - 3/32 inch Hex Wrench PARTS REQUIRED: Water Inlet Valve Actuator CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. The nitrogen supply should be turned off and the lines vented to atmosphere. PROCEDURE: 1. Remove the five (5) screws from the rear of the Module using the ¼ inch Nut Driver. 2. Remove the cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 3. Remove the (4) screws holding the Actuator to the Front Panel. 4. Loosen the set-screw on the Actuator Key and remove from the Actuator. 5. Install the Actuator Key onto the new Actuator. 6. Twist the Actuator Key so that the flat side of the Actuator Shaft is positioned upwards. 7. Install the new Actuator into the SPE-DEX Remove the nitrogen hoses from the old Actuator and install on the new Actuator. It is recommended that this be done one at a time. If both were removed earlier, follow one of the nitrogen lines to the MAC Valve. The line coming from the A port on the MAC valve goes into the top port on the Actuator, while the B hose goes into the lower port on the Actuator. 45

47 46

48 7.8. Replacing the Collection Adapter ( ) PURPOSE: This work instruction will replace the MAC Valve in a SPE-DEX 4790 Module. TOOLS: - ¼ inch Wrench or Adjustable Wrench - Small Standard Screwdriver - Small Phillips Screwdriver - Small Wire Cutters PARTS REQUIRED: Collection Adapter CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. PROCEDURE: 1. Using a standard screwdriver, remove the (3) screws which hold the Check Valve into the Collection Adapter and set the Check Valve aside if it is going to be used again. 2. Press down on the front platform on the SPE-DEX 4790 and, using a Phillips screwdriver, unscrew the Collection Adapter from the platform. 3. Cut through one of the two vacuum lines attached to the Collection Adapter. 4. Remove the remaining tubing on the barb fitting so the barb may be reused. 5. Using a Wrench, unscrew both barb fittings from the Collection Adapter. 6. Screw both barb fittings into the new Collection Adapter and reattach the trimmed tubing to the barb. 7. Reattach the Collection Adapter to the platform. Care should be taken not to overtighten the screws or they may strip the threads within the hole. 8. Install a Check Valve into the Collection Adapter. 47

49 7.9. Replacing the MAC Valve ( ) PURPOSE: This work instruction will replace the MAC Valve in a SPE-DEX 4790 Module. TOOLS: - ¼ inch Nut Driver - 5/16 inch Wrench - Molex Pin Extraction Tool ( ) - Small Standard Screwdriver - Small Phillips Screwdriver - Small Wire Cutters PARTS REQUIRED: MAC Valve Molex Pin Extraction Tool CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. The nitrogen supply should be turned off and the lines vented to atmosphere. PROCEDURE: 9. Remove the five (5) screws from the rear of the Module using the ¼ inch Nut Driver. 10. Remove the cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 11. Locate the MAC valve and follow the black wires to the Molex connector. 12. The pin layout for the Molex connector half attached to the valves is shown below. Note that the locking mechanism is shown up. 48

50 Press Here to Unlock Using the Molex Extraction Tool, remove pins 7 and 8 (the pins which go to the black wires). 14. Using wire cutters, clip the black tubing going into the MAC Valve. 15. Remove the two screws that hold the MAC Valve onto the SPE-DEX 4790 chassis. 16. Install the nitrogen tubing onto the MAC Valve: a. The line going into port A on the MAC Valve should come from the top fitting on the Water-Inlet Valve Actuator. b. The line going into port B on the MAC Valve should come from the bottom fitting on the Water-Inlet Valve Actuator. c. The bottom line extends to the gas supply port on the bulkhead of the SPE-DEX With the tubing attached, replace the screws which hold the MAC Valve in place. 18. Plug the electrical pins into the Molex connector taking care to orient the pin such that the u-channel aligns with the post inside the Molex connector. The pin will only fit in one direction. 19. Attach both halves of the Molex connector together. 20. Set the MAC Valve by performing the following: 49

51 a. Locate the IN (left one shown in photo) and EX brass screws on the MAC Valve. b. The IN screw controls the rate of open motion of the rotary actuator. c. Turn the brass colored EX screw completely counter-clockwise. d. Next, turn the brass colored IN screw completely clockwise, and then back out one turn. 21. Reattach the nitrogen source to the extractor and the controller and power on the unit. 22. Within the Envision Controller, load and run the Sample Drain method. Observe the Water-Inlet Valve Actuator Shaft (directly in the middle of the Actuator) and confirm that the shaft rotates 90 degrees in one second. If necessary, adjust the IN screw in quarter-turn increments to increase the speed that the valve opens. 23. Place the extractor cover on to the extractor, making sure that the front tabs are in place. 24. Starting with the top middle hole, replace the screws, but so not fully tighten until all screws are in place. 50

52 7.10. Replacing the Exhaust Fan (PN ) PURPOSE: This work instruction will replace the Exhaust Fan in a SPE-DEX 4790 Module. TOOLS: - ¼ inch Nut Driver - 5/16 inch Wrench PARTS REQUIRED: Exhaust Fan CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. PROCEDURE: 1. Remove the five (5) screws from the rear of the Module using the ¼ inch Nut Driver. 2. Remove the cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 3. Locate the Exhaust Fan and, following the power wires, unplug the Fan from the Control Circuit Board. 4. Remove the four nuts that hold the Exhaust Fan to the Chassis using the Wrench. 5. Install the new Exhaust Fan making sure to run the power wires through the retention clip. 6. Plug the Exhaust Fan into the Control Circuit Board 7. Reinstall the cover using the screws removed in step Procedure complete. 51

53 7.11. Replacing the Spring Motor (PN ) PURPOSE: This work instruction will replace the Spring Motor in a SPE-DEX 4790 Module. TOOLS: - ¼ inch Nut Driver - 5/16 inch Hexagonal Wrench - Short Phillips Screwdriver PARTS REQUIRED: Spring Motor CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. Any glassware attached as a Collection Vessel should be removed. PROCEDURE: 1. Remove the five (5) screws from the rear of the Module using the ¼ inch Nut Driver. 2. Remove the cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 3. Remove the screw from the rear of the Platform mount as seen in the above picture. Care should be taken with this as the Spring Motor Attachment is what holds the Platform up. 4. Allow the Spring Motor Wire to retract past the Pivot Wheel until the spring rests again the Spring Motor. 52

54 5. Locate the mounting bracket and loosen the 5/16 hexagonal nut and the screw, circled in red. 6. Swing the valve mounting bracket out of the way and, using a screwdriver, remove the four screws from the Spring Motor. 7. Install the new Spring Motor. 8. Pull the Spring Motor Wire over the Pivot Wheel and attach to the Platform. 9. Reinstall the mounting bracket and replace the cover. 53

55 7.12. Replacing the IR Photointerruptor (PN ) PURPOSE: This work instruction will replace the IR Photointerruptor in a SPE-DEX 4790 Module. TOOLS: - ¼ inch Nut Driver PARTS REQUIRED: IR Photointerruptor CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. PROCEDURE: 1. Remove the five (5) screws from the rear of the Module using the ¼ inch Nut Driver. 2. Remove the cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 3. Locate the Photointerruptor and remove the two nuts using the ¼ inch Nut Driver. 4. Unplug the old Photointerruptor and install the new Photointerruptor using the nuts. 5. Reinstall the cover using the screws removed in step Procedure complete. 54

56 7.13. Replacing the SPE-DEX 4790 Keypad (PN CE) PURPOSE: This work instruction will replace the Keypad on a SPE-DEX 4790 Module. TOOLS: - ¼ inch Nut Driver - Phillips Screwdriver PARTS REQUIRED: CE Keypad Overlay CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. PROCEDURE: 1. Remove the five (5) screws from the rear of the Module using the ¼ inch Nut Driver. 2. Remove the cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 3. Unplug the Keypad from the Control Circuit Board. 4. Using a screwdriver, remove the two screws on the front end of the Control Circuit Board; loosen the two screws in the rear. 5. With the screwdriver, push on the rear of the Keypad until it pulls away from the sheet metal. 55

57 6. Pull the old Keypad Ribbon upwards and out through the hole in the chassis. 7. Insert and run the Keypad Ribbon through the chassis and route it behind the Control Circuit board. 8. Tighten or replace all screws to the Control Circuit Board making sure to attach the KeyPad s Grounding Wire to the bottom front screw. 9. Replace the cover. 56

58 7.14. Replacing the Control Circuit Board (PN CE) PURPOSE: This work instruction will replace the Control Circuit Board on a SPE-DEX 4790 Module. TOOLS: - ¼ inch Nut Driver - Phillips Screwdriver PARTS REQUIRED: CE Keypad Overlay CAUTIONS: Prior to beginning, the Controller should be turned off and the Module should have the data/power cable unplugged. PROCEDURE: 1. Remove the five (5) screws from the rear of the Module using the ¼ inch Nut Driver. 2. Remove the cover by holding the bottom of each side and pushing the bottom backwards to disengage the front locking tabs. Lift the cover off. 3. Unplug the Keypad, Ribbon cable, both Thermistors, the IR Sensor, and the Exhaust Fan from the Control Circuit Board. 4. Using a screwdriver, remove the four (4) screws holding the Control Circuit Board 5. Replace the Control Circuit Board with the new part and tighten all screws making sure to attach the KeyPad s Grounding Wire to the bottom front screw. 6. Attach the Keypad, Ribbon cable, the IR Sensor, and the Exhaust Fan to their labeled ports on the Control Circuit Board. 7. When replacing the Thermistors, refer to the table below: When Facing Extractor Thermistor Height Main Circuit Board Connector Right Side Top J6 Left Side Bottom J7 8. Replace the cover. 57

59 8. Schematics 8.1. Nitrogen and Solvent Delivery System 58

60 8.2. Waste and Vacuum System 59

61 8.3. Plumbing Schematics MAC Valve and Water Inlet Valve Actuator B A Water Inlet Valve Actuator MAC Valve Supply Water Inlet Valve Status MAC Valve Line A MAC Valve Line B Closed Not Pressurized Pressurized Open Pressurized Not Pressurized 60

62 Prewet and Rinse Plumbing Valve Manifold Water Inlet Valve Assembly PW 1 PW 2 PW 3 Supply PW 5 PW 4 R 1 R 2 R 3 61

63 Vacuum Plumbing Modified Luer Plug Collection Adapter Solvent Waste Valve Water Waste Valve Elution Valve To Waste 62

64 8.4. Electrical Systems Ribbon Cable All circuits within this table are run at 24 Volts. Wire Color Conn. A Pins Conn. B Pins Conn. C Pins Conn. D Pins Use Brown 1 Not Used Red 2 1 Not Used Orange 3 3 Prewet 2 Yellow 4 2 Not Used Green 5 2 Solvent-Collect Blue 6 8 MAC Violet 7 9 Prewet 5 Grey 8 6 Water-Waste White 9 15 Rinse 3 Black 10 6, J-12* Prewet 3, Rinse 1 Brown 11 1 Prewet 1 Red 12 8, J-14* Prewet 4, Rinse 2 Orange 13 3 Solvent-Waste Yellow 14 4 Solvent-Waste Green 15 7 Prewet 4 Blue 16 2, J-16* Prewet 1, Rinse 3 Violet Rinse 2 Grey 18 4 Prewet 2 White 19 Not Used Black Prewet 5 Brown 21 5 Water-Waste Red 22 1 Solvent-Collect Orange 23 5 Prewet 3 Yellow 24 7 MAC Green Rinse 1 Blue 26 Not Used *Wire utilizes a jumper to connect the circuits. 63

65 Conn. D Conn. C Conn. A Conn. B Conn. A Conn. B Conn. C Conn. D 64

66 Control Circuit Board Valve Indicators There are 16 LEDs in the top right of the Control Circuit Board. These LEDs can be used to tell if a signal is being given for a valve to fire. The LEDs are highlighted in red below. LED 1 Rinse 4 LED 9 Not Used LED 2 Sample-Collect LED 10 Prewet 2 LED 3 Rinse 3 LED 11 Prewet 5 LED 4 Solvent-Waste LED 12 Prewet 1 LED 5 Rinse 2 LED 13 Prewet 4 LED 6 Water-Waste LED 14 Not Used LED 7 Rinse 1 LED 15 Prewet 3 LED 8 Water Inlet Valve LED 16 Prewet 6 65