Come spingere al limite le misure degli ormoni steroidei per LC-MS/MS.

Transcription

1 Come spingere al limite le misure degli ormoni steroidei per LC-MS/MS. Bruno Casetta AB Sciex Consultant Clinical Applications Mass Spectrometry AB SCIEX 1

2 Il problema della accuratezza degli IAs L ack o f S p ecificity o f Im m u n o assays D ata fro m C A P P T P ro g ram A n a lytes L ow H ig h 17 O H P rogesterone(ng/dl) (5.2) E stradiol (pg/m L) (17.1) P rogesterone (ng/m L) (3.0) AB SCIEX Il pattern metabolico degli steroidi AB SCIEX 2

3 Steroid profiling by MS/MS in blood spots Cortisol d8-17oh-progesterone (IS) Control D4-Androstenedione 17OH-Progesterone False positive for CAH CAH From: Jean M. Lacey, Carla Z. Minutti, Mark J. Magera, Angela L. Tauscher, Bruno Casetta, Mark McCann, James Lymp, Si Houn Hahn, Piero Rinaldo,and Dietrich Matern, Clin Chem 2004; 50: AB SCIEX??? AB SCIEX TOF/TOF Systems AB SCIEX TripleTOF 5600 System QTRAP 5500 System 4000 QTRAP System itraq reagents Scheduled MRM Algorithm mtraq reagents AB SCIEX 3

4 Il Triplo Quadrupolo Q0 Q1 Q2 Q3 Q0 Q1 Q2 Q3 LIT Mol.Ion Fragmention Fragm.Ions AB SCIEX Stable and unstable Ion trajectories At any RF/DC potential setting only ions of one particular mass/charge ratio have a stable trajectory AB SCIEX 4

5 Multiple Reaction Monitoring Pass Precursor CAD Check for marker Signal after Q3 Q1 LINAC Q2 Q3 m/z Ionized Analyte Specific fragment Nel Quadrupolo in SIM o SRM, il Duty Cycle è vicino al 100%: tutti gli ioni prodotti dalla sorgente sono utilizzati per acquisire la misura AB SCIEX The workflow for measurements with MSMS Affordable Sample Prep LC desires/requ. BLOOD LC/MS/MS instrument with maximum Sensitivity and Robustness Specimen Amount AB SCIEX 5

6 17-OHP performances Kushnir 2006b Serum/Plasma SPE, NH2OH, LLE API 4000-TIS-pos 200 ul 40 pg/ml 50 pg 2 Ceglarek 2008 Serum PPT, 2D API 4000-APCI-pos 100 ul 33 pg/ml 100 pg 3.3 Lacey 2004 DBS-4.8mm LLE API 3000-TIS-pos 10 ul 4 pg/ml 1900 pg 7.6 Guo 2006 Serum PPT, false2d API 5000-APPI-pos 200 ul 135 pg/ml 1.5 pg Rauh 2006 Serum/Plasma/saliva PPT, 2D API 4000-APCI-pos 100 ul 20 pg/ml 100 pg 2 Janzen 2008 Serum/DBS PPT, dry W Ultima-ESI-pos 15 ul 12 pg/ml 325 pg 3.9 Fanelli 2010 Serum PPT,off-line SPE,2D API 4000-APCI-pos 900 ul 600 pg/ml 9.8 pg 1.7 imethod Serum PPT, 1D diverted API 5X00-APCI-pos 200 ul 33 pg/ml 100 pg 3.3 PE-Kit Serum PPT, dry API 5000-APCI-pos 100 ul 80 pg/ml 38 pg 3.04 LOQ AB SCIEX 17-OHP performances Kushnir 2006b Serum/Plasma SPE, NH2OH, LLE API 4000-TIS-pos 200 ul 40 pg/ml 50 pg 2 Ceglarek 2008 Serum PPT, 2D API 4000-APCI-pos 100 ul 33 pg/ml 100 pg 3.3 Lacey 2004 DBS-4.8mm LLE API 3000-TIS-pos 10 ul 4 pg/ml 1900 pg 7.6 Guo 2006 Serum PPT, false2d API 5000-APPI-pos 200 ul 135 pg/ml 1.5 pg Rauh 2006 Serum/Plasma/saliva PPT, 2D API 4000-APCI-pos 100 ul 20 pg/ml 100 pg 2 Janzen 2008 Serum/DBS PPT, dry W Ultima-ESI-pos 15 ul 12 pg/ml 325 pg 3.9 Fanelli 2010 Serum PPT,off-line SPE,2D API 4000-APCI-pos 900 ul 600 pg/ml 9.8 pg 1.7 imethod Serum PPT, 1D diverted API 5X00-APCI-pos 200 ul 33 pg/ml 100 pg 3.3 PE-Kit Serum PPT, dry API 5000-APCI-pos 100 ul 80 pg/ml 38 pg 3.04 Instrument AB SCIEX 6

7 17-OHP performances Kushnir 2006b Serum/Plasma SPE, NH2OH, LLE API 4000-TIS-pos 200 ul 40 pg/ml 50 pg 2 Ceglarek 2008 Serum PPT, 2D API 4000-APCI-pos 100 ul 33 pg/ml 100 pg 3.3 Lacey 2004 DBS-4.8mm LLE API 3000-TIS-pos 10 ul 4 pg/ml 1900 pg 7.6 Guo 2006 Serum PPT, false2d API 5000-APPI-pos 200 ul 135 pg/ml 1.5 pg Rauh 2006 Serum/Plasma/saliva PPT, 2D API 4000-APCI-pos 100 ul 20 pg/ml 100 pg 2 Janzen 2008 Serum/DBS PPT, dry W Ultima-ESI-pos 15 ul 12 pg/ml 325 pg 3.9 Fanelli 2010 Serum PPT,off-line SPE,2D API 4000-APCI-pos 900 ul 600 pg/ml 9.8 pg 1.7 imethod Serum PPT, 1D diverted API 5X00-APCI-pos 200 ul 33 pg/ml 100 pg 3.3 PE-Kit Serum PPT, dry API 5000-APCI-pos 100 ul 80 pg/ml 38 pg 3.04 Sample preparation AB SCIEX 17-OHP performances Kushnir 2006b Serum/Plasma SPE, NH2OH, LLE API 4000-TIS-pos 200 ul 40 pg/ml 50 pg 2 Ceglarek 2008 Serum PPT, 2D API 4000-APCI-pos 100 ul 33 pg/ml 100 pg 3.3 Lacey 2004 DBS-4.8mm LLE API 3000-TIS-pos 10 ul 4 pg/ml 1900 pg 7.6 Guo 2006 Serum PPT, false2d API 5000-APPI-pos 200 ul 135 pg/ml 1.5 pg Rauh 2006 Serum/Plasma/saliva PPT, 2D API 4000-APCI-pos 100 ul 20 pg/ml 100 pg 2 Janzen 2008 Serum/DBS PPT, dry W Ultima-ESI-pos 15 ul 12 pg/ml 325 pg 3.9 Fanelli 2010 Serum PPT,off-line SPE,2D API 4000-APCI-pos 900 ul 600 pg/ml 9.8 pg 1.7 imethod Serum PPT, 1D diverted API 5X00-APCI-pos 200 ul 33 pg/ml 100 pg 3.3 PE-Kit Serum PPT, dry API 5000-APCI-pos 100 ul 80 pg/ml 38 pg 3.04 Starting Sample Volume Sample Volume consumed On-column pg for LOQ AB SCIEX 7

8 A Steroids commercial Kit XIC of -MRM (4 pairs): Period 1, / Da ID: ALDO 1 from Sample 2 (Well 2-CoLOW) of 0307-Plate1-row3.wiff (Heated Nebulizer),... x 20.0 Max cps. 2.2e5 2.1e5 2.0e5 1.9e5 1.8e5 CORT 1.7e5 1.6e5 1.5e5 1.4e5 1.3e5 DHEAS Inte ns ity, c ps 1.2e5 1.1e5 1.0e5 9.0e4 8.0e4 7.0e4 6.0e4 5.0e4 4.0e4 ALDO CCONE 11-DECOL ADIONE TESTO 17-OHP DHEA 3.0e4 2.0e4 PROG 1.0e Time, min AB SCIEX Corticosteroids Positive Ion Product Ion Spectra +M S2 (347.30): 20 M CA scans from Sample 1 (21 Desoxy Pos) of APCI Product Ion wiff (Heated Nebulizer) Max. 2.5e5 cps e 5 2.4e 5 21-Desoxycortisol 2.2e 5 2.0e 5 1.8e 5 1.6e 5 1.4e 5 1.2e 5 1.0e e e e e m /z, am u +M S2 (347.50): 20 MCA scans from Sam ple 3 (Compound B pos) of APCI Product Ion wiff (Heated Nebulizer) Max. 1.9e6 cps. 1.9 e Corticosterone 1.8 e6 1.6 e6 1.4 e6 1.2 e6 1.0 e e e e e m /z, am u +M S2 (347.30): 20 MCA scans from Sample 5 (Com pound S ) of APCI Product Ion wiff (Heated Nebulizer) Max. 3.1e6 cps. 11-Desoxycortisol e6 2.8e6 2.6e e6 2.2e6 2.0e6 1.8e6 1.6e6 1.4e6 1.2e6 1.0e6 8.0e5 6.0e e e m /z, am u AB SCIEX 8

9 LC separation, why do we need it? 1.0e5 8.0e4 6.0e4 4.0e4 2.0e DESOX Isobaric Steroids Separation 17 OH PREGNENOLONE CORTICOSTERONE 11 DESOX Time, ANDROSTANEDIOL AB SCIEX Sample preparation for Steroids measurements with MSMS Off-line SPE Just Sample dilution LLE PPT and On-line purification AB SCIEX 9

10 Multiplex FIA - LC/MS/MS for NBS-CAH Autosampler AB Sciex 5500 System withturbov TIS Source Pump (FIA) Pump (LC) AB SCIEX The sequence FIA - LC/MS/MS Autosampler FIA (NBS) LC (CAH) FIA (NBS) AB SCIEX 10

11 +NL (46.00): Period 1, Exp 1, to min from Sample 14 (Sample5-Vial2) of ag06-protocol.wiff (Turbo Spray) 5.3e5 5.0e5 4.5e5 4.0e5 3.5e5 3.0e5 2.5e5 2.0e5 1.5e5 1.0e5 5.0e Max. 5.3e5 cps m/z, Da Principle of multiplexing NBS and CAH XIC of +MRM (5 pairs): Period 2, 363.3/121.1 Da ID: Cortisol from Sample 3 (Mix80nM-Phe1uM) of ag03-fia8020-a0_3b1_1unwashed.wiff (Turbo... x 5.0 Max. 3.1e4 cps. 6.9e5 6.5e5 6.0e5 5.5e5 5.0e5 4.5e5 4.0e5 3.5e5 3.0e5 2.5e5 2.0e5 1.5e e5 5.0e Time, min AB SCIEX XIC of +MRM (5 pairs): Period 2, 363.3/121.1 Da ID: Cortisol from Sample 14 (Sample5-Vial2) of ag06-protocol.wiff (Turbo Spray), Smoothed, S... x e5 2.4e5 Cortisol 21-deoxycortisol Max cps. 2.2e5 2.0e5 1.8e5 1.6e deoxycortisol Androstenedione 1.4e5 1.2e5 1.0e5 17-OH-Progest. 8.0e e4 4.0e4 2.0e4 0.0 Underiv. AA and AC Time, min AB SCIEX 11

12 Comparison with performances retreived from published papers. ANALYTE Reference Matrix Strategy Instrument Starting Specimen Volume Injected Specimen Volume ul LOQ units value On column units value Progestagens (21C) 17-Hydroxyprogesterone (17OHP) Kushnir 2006b Serum/Plasma SPE, NH2OH, LLE API 4000-TIS-pos 200 ul 40 pg/ml 50 pg 2 17-Hydroxyprogesterone (17OHP) Ceglarek 2008 Serum PPT, 2D API 4000-APCI-pos 100 ul 33 pg/ml 100 pg Hydroxyprogesterone (17OHP) Lacey 2004 DBS-4.8mm LLE API 3000-TIS-pos 10 ul 4 pg/ml 1900 pg Hydroxyprogesterone (17OHP) Guo 2006 Serum PPT, false2d API 5000-APPI-pos 200 ul 135 pg/ml 1.5 pg Hydroxyprogesterone (17OHP) Rauh 2006 Serum/Plasma/Saliva PPT, 2D API 4000-APCI-pos 100 ul 20 pg/ml 100 pg 2 17-Hydroxyprogesterone (17OHP) NBS-CAH DBS-5mm Extr. DBS, Mpx API 5500-TIS-pos 12 ul 1 pg/ml 2000 pg AB SCIEX Vitamin D analysis by LC/MS/MS 12

13 7-dehydrocholesterol Cholecalciferol (Vitamin D3) LIVER 25(OH)D 3 UVB 25(OH)D is the accepted measure of vitamin D nutritional status 7-dehydrocholesterol UVB LIGHT Cholecalciferol (Vitamin D3) LIVER 25(OH)D KIDNEY 1,25(OH) 2 Vitamin D 3 is the Calcium regulating hormone 1000 x LOWER then 25(OH)vitamin D AB SCIEX Colecalciferol Calcifediol Calcitriol AB SCIEX 13

14 Classical RIA methodology performances Imprecision: Sensitivity: Dynamic range: Reference range: 10 % CV to 15 % CV (between run) 24 pmol/l (10 pg/ml) pmol/l ( pg/ml) pmol/l (20-80 pg/ml) Sample volume: 1.5 ml serum RIA liquid scintillation counting (tritium) UZ Leuven Gasthuisberg Belgium AB SCIEX MATERIALS The hardware configuration as depicted in the schematic includes: - an AB SCIEX Triple Quad 5500 Mass Spectrometer equipped with a Turbo V source and the Electrospray probe, and with - an external 10 port-2 way Valco-valve - an LC-stack including: - 2 binary pumps - autosampler - a Perfusion POROS column R1/ x 50 mm. - two Onyx columns 3 x 100 mm AB SCIEX 14

15 1,25(OH) 2 -Vitamin D 2/3 (and optionally 25(OH)-Vitamin D 2/3 ) measurement with Sample On-Line Cleanup. Monolithic columns Autosampler Waste POROS column H 2 O / MetOH + Li + AB SCIEX Triple Quad 5500 MASS SPECTROMETER WITH ESI SOURCE H 2 O / ACN-MetOH Mix LOADING Pump SEPARATION Pump AB SCIEX The Di-Hydroxy-Vit.D 3 isomers separation (S),26(OH) 2 -Vit.D 3 d6-1α,25(oh) 2 -Vit.D (R),25(OH) 2 -Vit.D 3 1α,25(OH) 2 -Vit.D Time, min Time, min AB SCIEX 15

16 Data from external Lab. Imprecision (n=11 between day) Concentration (pg/ml) %CV Stability of the medium control (120 pmol/l 50 pg/ml) Start of series Mid series End of series UZ Leuven Gasthuisberg Belgium Method comparison n=76 UZ Leuven Gasthuisberg Belgium UZ Leuven Gasthuisberg Belgium AB SCIEX Performance keypoints A reduced sample size of only 30 ul of plasma is consumed for the reliable quantitation of calcitriol The LOQ achievable is < 10 pg/ml (< 24 pmol/l), The LOD is ~ 3 pg/ml (< 7 pmol/l) The intra-run precisions have been < pg/ml (48 pmol/l) and better than 5 % at 100 pg/ml (240 pmol/l). Bruno CASETTA, Ivo JANS, Jaak BILLEN, Dirk VANDERSCHUEREN and Roger BOUILLON European Journal of Mass Spectrometry, Volume 16, (2009) AB SCIEX 16

17 Serum E1 and E2. A first methodology for the E1 and E AB SCIEX 17

18 The 5 pg/ml-spiked serum sample AB SCIEX The approach with a 2D Sample Focusing Injection and On-Line Cleanup for LLE. Trapping column Analytical column Built-in Valve B Autosampler A Waste H 2 O + F- / MetOH AB SCIEX TRIPLE QUADRUPOLE MASS SPECTROMETER WITH ESI SOURCE H 2 O (A) / MetOH-ACN (B) LOADING Pump SEPARATION Pump AB SCIEX 18

19 Low serum sample XIC chromatogram as integrated by MultiQuant software for E AB SCIEX Calibration with New Method on E2-spiked serum samples AB SCIEX 19

20 Correlation New Method with IA 300 pg/ml LC-MSMS y = x R 2 = Series1 Linear (Series1) pg/ml IA AB SCIEX Sample Prep. 1 Vol. Serum 2 Vol. ACN AB SCIEX 20

21 Endogenous E1, E2 in Plasma by PPT and 2D- XIC of of -MRM (4 pairs): / / Da ID: d4e1 from Sample 22 (PlasmaACN) of of Plasma-C8.wiff (Turbo Spray), Smoothed, Smoothe... XIC of -MRM (4 pairs): / / Da ID: E2 E1 from Sample 2 (PlasmaACN) (Ex 25 ppb) of of pg-C8.wiff Plasma-C8.wiff (Turbo (Turbo Spray) Spray), Smoothed,... approach, Option 1 3.1e6 3.0e XIC of MRM 1300 (4 pairs): / Da ID: E1 from e6 3.1e e e e e6 2.6e e e e6 2.2e e e e61.6e e6 1.4e e e6 1.2e e6 1.0e e e e5 6.0e E1 E1 E2 E Max. 3.1e6 cps. I n t e n s i t y, c p s I n t e n s i t y, c p s I n t e n s i t y, c p s XIC of -MRM (4 pairs): / Da ID: d4e1 f... Max cps Time, min XIC of -MRM (4 pairs): / Da ID: E1 fro... Max cps. 4.0e5 4.0e e5 2.0e Time, 7.5 min Time, 10.4 min Time, min Time, min Time, min XIC of -MRM (4 pairs): / Da ID: E2 fro... Max cps Plasma E1 E Max cps. Max. Max e6 cps AB SCIEX Endogenous E1, E2 in Plasma by PPT and 2Dapproach, Option 2 Intensity, cps XIC of -MRM 2800 (4 pairs): / Da ID: E1 from e4 9.5e5 1.08e6 1.05e e6 9.0e5 1.00e4 1.00e e e5 8.0e5 9.00e e5 8.00e5 7.50e5 7.00e5 6.50e5 6.00e5 5.50e5 5.00e5 4.50e5 4.00e5 3.50e5 3.00e5 2.50e5 2.00e5 1.50e5 1.00e5 5.00e XIC of -MRM (4 pairs): / Da ID: E2 from Sample 2 (PlasmaACN) of Plasma-PF35t90i6.wiff (Turbo Spray), Sm... XIC of -MRM (4 pairs): / Da ID: d4e2 from Sample 2 (PlasmaACN) of Plasma-PF35t90i6.wiff (Turbo Spray), S... XIC of -MRM (4 pairs): / Da ID: E1 from Sample 4 (Ex 25 ppb) of pg-PF35t90i6.wiff (Turbo Spray), Smoothe e e e e e e e e e e e e e e5 E E1 E2 Max. 1.1e6 cps E2 I n t e n s i t y, c p s I n t e n s i t y, c p s I n t e n s i t y, c p s XIC of -MRM (4 pairs): / Da ID: E1 fro... Max cps Time, min XIC of -MRM (4 pairs): / Da ID: E1 fro... Max cps e min Time, 7.5 min Time, 9.5 min Time, Time, min Time, min AB SCIEX 1.00e Time, min XIC of -MRM (4 pairs): / Da ID: E2 fro... Max cps Plasma E2 E E Max cps. Max. 4.5e4 cps. Max. 9.5e5 cps

22 SelexION Ion Mobility Technology: Introducing a New Dimension of Selectivity for LC-MS/MS Analysis SelexION Ion Mobility Device Separates analyte ions based upon differences in their low-field and high-field mobilities. New hardware The application of alternating low and high electric fields in the region between two planar electrodes causes analyte ions to migrate towards one of the electrodes. Ions are steered back towards the center-line using a compound-specific DC compensation voltage AB SCIEX 22

23 SelexION Ion Mobility Device Separates analyte ions based upon differences in their low-field and high-field mobilities. The application of alternating low and high electric fields in the region between two planar electrodes causes analyte ions to migrate towards one of the electrodes. Ions are steered back towards the center-line using a compound-specific DC compensation voltage. ions in transport gas to mass spectrometer Compensation Voltage (COV) AB SCIEX Tuning the SelexION Ion Mobility Device t 1 t 2 α=0 α>0 α<0 K( E) K(0) α( E) = K(0) Recall that ions migrate towards one of the planar electrodes If an ion s high-field mobility K(E) is larger than its low-field mobility K(0), α>0 If an ion s low-field mobility K(0) is larger than it high-field mobility K(E), α<0 Any ion can be steered back onto the center-line, by application of a compound-specific DC compensation voltage (CV) AB SCIEX 23

24 Tuning the SelexION Ion Mobility Device 100% Rel. Int. (%) 50% 0% Compensation Voltage (CV) In infusion mode, the compensation voltage (CV) parameter is ramped in order to determine the optimized value. Note that this is analogous to tuning any other compound-specific parameter, e.g. the collision energy (CE) AB SCIEX LC/MS/MS Analysis of Prostaglandins using SelexION Ion Mobility Technology for Enhanced Selectivity (a) SelexION device off PGF 2α 8-iso-PGF 2α (b) SelexION device on COV = 10 PGF 2α PGF 2α HO OH O CH 3 HO OH (c) SelexION device on COV = 7 8-iso-PGF 2α 8-iso PGF 2α HO OH O CH 3 HO OH AB SCIEX 24

25 Optimization of the SelexION Ion Mobility Device Optimization of the SelexION device for isomers is accomplished by ramping the COV parameter. In the example shown, at COV=10, only PGF 2α is transmitted. At COV=7, only 8-iso-PGF 2α is transmitted. 8-iso PGF 2α OH PGF 2α OH O O HO HO CH 3 CH 3 HO OH HO OH Chromatographic run-time (minutes) AB SCIEX Testosterone Female Serum Sample: PPT Enhanced Selectivity using SelexION Technology DMS OFF 289/109 Peak of interest DMS OFF 289/97 Peak of RT 3.37 min DMS ON 289/109 DMS ON 289/ AB SCIEX 25

26 Application examples using SelexION Ion Mobility Technology AB SCIEX Enhanced separations using chemical modifier Modifier is introduced by a precision liquid delivery pump Typical modifiers include: isopropanol, acetone, acetonitrile, methanol Nitrogen-only transport gas Peak Capacity compounds: methylhistamine, minoxidil, ephedrine, norfentanyl, acyclovir, clenbuterol, tramadol, quinoxifen, pamaquin, fendiline, buscopan % isopropanol in transport gas Peak Capacity 40 Normalized Signal CV (V) 2011 AB SCIEX 26

27 Effects of Different Modifiers Normalized Signal Isopropanol Separation Normalized Signal CV (V) Acetone Separation Red: Ephedrine Black: Acyclovir Green: Norfentanyl Blue: Clenbuterol Purple: Imipramine Brown: Diazepam CV (V) Pink: Quinoxifen Normalized Signal Acetonitrile Separation CV (V) AB SCIEX Thank you bruno.casetta@absciex.com 27