Introducing the SCIEX Triple Quad and QTRAP LC-MS/MS System with optional SelexION + Technology
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1 RUO-MKT For Research Use Only. Not for use in diagnostic procedures Introducing the SCIEX Triple Quad and QTRAP LC-MS/MS System with optional SelexION + Technology SCIEX, Concord, Ontario (Canada)
2 Our Best Triple Quad Just Got Better the Most Sensitive and Fastest SCIEX Triple Quad System The New SCIEX Series with IonDrive Technology New Improved IonDrive detection system Detect analytes across a wide scope of chemistries, low mass or high mass, positive or negative polarity Quantify with lowest limits of quantitation and up to 6 orders of linear dynamic range Ultra fast polarity switching with Scheduled MRM Pro algorithm for multi-target quantitation Elevated SelexION technology Jet Injection technology with improved transmission and no effect on resolution Audible noise reduction New New Industry proven performance of IonDrive Turbo V ion source and QJet ion guide Results in less time fast, high-throughput, simplified data processing tools Service and support resources for success AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
3 Hardware Overview RUO-MKT For Research Use Only. Not for use in diagnostic procedures
4 Improved IonDrive Detection System New detector Larger detection area New power supply High energy dynode at 15 kv improves sensitivity Enables faster 5 msec polarity switching Floated design Ions in negative polarity directed straight into the detector This benefits low mass ions which are detected much more efficiently than in the 6500 system Contributes to significant signal gains in negative mode Q3 fragment ion <100 Da S/N (LOQ) improvements are compound, matrix and LC dependent Improved data quality in screening applications with ultra-fast 5 msec polarity switching AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
5 Sensitivity of System in Positive Polarity 0.1 pg/ml Testosterone (with SelexION + Technology, 50 µl Injection) AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
6 Linearity of System in Positive Polarity 0.1 to 100 pg/ml Testosterone (with SelexION + Technology) 0.1 to 5 pg/ml Accuracies: 92 to 109% %CV: 3.8% at 0.1 pg/ml AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
7 Linearity of System in Positive Polarity 1 to 100,000 pg/ml Flurazepam (with SelexION + Technology) 1 to 1000 pg/ml 5 orders r > AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
8 Sensitivity of System in Negative Polarity 1 ng/l (1 part-per-trillion) PFOA and PFOS (Linearity 0.5 to 5000 ng/l) PFOA 413/ / /219 Quantifier (blue) Qualifier 1 (purple) Qualifier 2 (orange) PFOS 499/80 499/99 Quantifier (blue) Qualifier 1 (purple) AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
9 Sensitivity of System in Negative Polarity 100 ppt PFBS QTRAP 5500 LC-MS/MS system QTRAP LC-MS/MS system MRM / 80.0 ~ 2x S/N improvement MRM / 99.0 ~ 2.5x S/N improvement AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
10 Sensitivity of System in Negative Polarity 0.1 ng/ml (100 ppt) Monofluoroacetate (Linearity ng/ml) 77/57 (blue) 77/33 (purple) Internal standard 81/60 (blue) 81/35 (purple) Accuracies: 96.1 to 110.9% %CV: 4.99% at 0.1 pg/ml (n = 10) AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
11 Ultra Fast Polarity Switching of System Multi-Pesticide Screening for ~300 Analytes with 5 msec Switching 552 MRM transitions Positive ESI Flufenacet CV = 4.37% Methoxyfenozide CV = 1.52% 58 MRM transitions Negative ESI AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
12 Multi-Pesticide Screening with 5 msec Polarity Switching Pesticide Standard and Sample Extracts (Positive Polarity) 1 ng/ml Tomato (10x) Cucumber (10x) Banana (10x) Strawberry (10x) Lemon (10x) Arugula (20x) AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
13 Multi-Pesticide Screening with 5 msec Polarity Switching Pesticide Standard and Sample Extracts (Negative Polarity) 1 ng/ml Tomato (10x) Cucumber (10x) Banana (10x) Strawberry (10x) Lemon (10x) Arugula (10x) AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
14 Software Overview RUO-MKT For Research Use Only. Not for use in diagnostic procedures
15 Quantitation and Ion Ratio in MultiQuant Software QuEChERS Extract of Cucumber (dspe with PSA) and 10 x Dilution Propamocarb Std. 1 Lemon Tomato Cucumber Strawberry Arugula AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
16 QTRAP Library Searching in MasterView Software QuEChERS Extract of Cucumber (dspe with PSA) and 10 x Dilution Sample Standard MS/MS FIT = 100% AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
17 Quantitation and Ion Ratio in MultiQuant Software QuEChERS Extract of Strawberry (dspe with PSA) and 10 x Dilution Dimethoate Std. 1 Lemon Tomato Cucumber Strawberry Arugula AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
18 QTRAP Library Searching in MasterView Software QuEChERS Extract of Strawberry (dspe with PSA) and 10 x Dilution Sample Standard MS/MS FIT = 100% AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
19 Quantitation and Ion Ratio in MultiQuant Software QuEChERS Extract of Strawberry (dspe with PSA) and 10 x Dilution Fludioxonil (-) Std. 1 Lemon Tomato Cucumber Strawberry Arugula AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
20 SelexION + Technology Differential Mobility Spectrometry (DMS) RUO-MKT For Research Use Only. Not for use in diagnostic procedures
21 Lens Lens SelexION + Ion Mobility Device with Jet Injector Technology New DMS cell designed to improve ion transmission by ~2x without loss in selectivity or resolution Addition of lens increases ion velocities into cell Reduces transit times through detrimental fringing fields SelexION cell Lens DMS Electrode 1 DMS Electrode 1 DMS Electrode 2 DMS Electrode 2 DMS Electrode 1 DMS Electrode 1 Only available on platform SelexION + cell Lens DMS Electrode 2 DMS Electrode 2 Continually driving selectivity improvements AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
22 Detection of Triazole with and without DMS with DMS DMS removed S/N ~ 60 Standard 0.5 ng/l N NH N Spiked and extracted fruit (10 µg/kg) S/N ~ 390 IS = 3000 V; TEM = 550ºC AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
23 Linearity and Repeatability (0.01 mg/kg 50 mg/kg; n = 3) Accuracy % %CV < 2% AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
24 Added Selectivity to Simplify Sample Extraction Example: 1 µg/kg Imidacloprid and Metabolites in Bee Pollen Analyzed using QTRAP System with SelexION + Technology Imidacloprid Imidacloprid-IS 5-OH Olefin Pollen extract 4x dilution, average sensitivity gain over 6500 with DMS = 3x AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
25 Detection of Somatostatin with and without SelexION with DMS DMS removed 100 pg/ml Somatostatin, a cyclic peptide with poor MS/MS fragmentation is acquired using MIM (819.7/ 819.7) assay in rat plasma showing high background noise limiting the S/N ratio and LOQ Highly reduced background noise enabling desirable LOQ of 100 pg/ml using new SelexION + jet injector on System (S/N ratio = 12, accuracy = 101% and %CV < 10% ) AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
26 Industry Proven Robustness and Ruggedness SelexION +, IonDrive Turbo V Ion Source and QJet Ion Guide Alprazolam %CV = 3.5% Buprenorphine %CV = 7.9% Clenbuterol %CV = 2.9% Methamphetamine %CV = 2.8% Repeatability of 4000 injections (crashed human plasma (1:3) diluted 1:2) AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
27 Industry Proven Robustness and Ruggedness 1. Injection Injection Injection Injection Injection Alprazolam Buprenorphine Clenbuterol Methamphetamine Repeatability of 4000 injections (crashed human plasma (1:3) diluted 1:2) AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
28 Audible Noise Reduction Active (source exhaust modifications) Source exhaust flow minimized to match gas load Current shipping design assumes maximum incoming source gas load at all times Passive (quieter tubing) Eliminate whistling noise Quieter system operation than 6500 Noticeable under most source conditions No impact to sensitivity Decreased audible noise for a more comfortable work environment AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
29 The New SCIEX Series and SelexION + technology Industry proven performance: IonDrive Turbo V ion source and QJet ion guide Significant sensitivity boost in low mass negative mode with new HED + detector technology Enhanced SelexION + performance with new jet injector DMS cell without compromising resolution Fast polarity switching speed (5 msec) Audible noise reduction AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
30 Trademarks/Licensing For Research Use Only. Not for use in diagnostic procedures AB Sciex. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX is being used under license AB Sciex RUO-MKT For Research Use Only. Not for use in diagnostic procedures
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