European Reference Laboratory - FV Amadeo R. Fernández-Alba Carmen Ferrer

Size: px

Start display at page:

Download "European Reference Laboratory - FV Amadeo R. Fernández-Alba Carmen Ferrer"

Grant Mitchell
5 years ago
Views:

6410 / 6490 Comparison Fenhexamid 302 > 55 Orange at 0.

3 6410 / 6490 Comparison Fenhexamid 302 > 55 Orange at mg/kg LC-QQQ-MS/MS > 55 Orange at mg/kg dilx10 LC-QQQ-MS/MS 6490 S/N: 10.7 S/N: > > 97

4 6410 / 6490 Comparison Aldicarb 213 > 89 Orange at mg/kg LC-QQQ-MS/MS > 89 Orange at mg/kg dilx10 LC-QQQ-MS/MS > > 116 S/N: 0.8 S/N: 21.1

6410 / 6490 Comparison Thiabendazole Apple at 0.010 mg/kg LC-QQQ-MS/MS 6410 Apple at 0.

5 6410 / 6490 Comparison Thiabendazole Apple at mg/kg LC-QQQ-MS/MS 6410 Apple at mg/kg dilx10 LC-QQQ-MS/MS > > 175 S/N: - S/N: > > 130

7 Fipronil MRL: mg/kg Standards in baby food Fipronil mg/kg LC-QQQ-MS/MS 6410 Fipronil mg/kg Dilx5 LC-QQQ-MS/MS 6490 MRM2 only noise S/N: 8.7

8 Fipronil MRL: mg/kg Standards in baby food Fipronil 0.001mg/kg Dilx5 Fipronil-desulfinyl 0.001mg/kg Dilx5 S/N: 8.7 S/N: 7.1

9 Cadusafos MRL: mg/kg 0.001mg/kg standard in baby food Dilx5 S/N: 24.2

10 Ethoprophos MRL: mg/kg mg/kg standard in baby food Dilx5 S/N: 11.3

11 Fensulfothion Shall not be used (Concentration level below mg/kg) mg/kg standard in baby food Dilx5 S/N: 5.7

12 Omethoate Shall not be used (Concentration level below mg/kg) mg/kg standard in baby food Dilx5 S/N: 6.5

13 Dilution of black tea extract Results for fuberidazole 1:50 Fuberidazole 1:10 1:5 good S/N even in 1:50 dilution increased signal 1:2 5 µg/kg 40 fg o.c. 200 fg o.c. 400 fg o.c. 1 pg o.c. 2 pg o.c. WEBEX triggered MRM October 23, 2012

Results for Fuberidazole Calibration in solvent and recoveries in different matrices Matrix Concentration Response in % Solvent 0.94 Black tea 0.

14 Results for Fuberidazole Calibration in solvent and recoveries in different matrices Matrix Concentration Response in % Solvent 0.94 Black tea Cucumber Lemon Pepper Rocket Tomato WEBEX triggered MRM October 23, 2012 Wheat flower

15 Results for Fuberidazole Recoveries for dilution in black tea Matrix Concentration Response in % Black tea Black tea 1 in Black tea 1 in Black tea 1 in WEBEX triggered MRM October 23, 2012

16 Dilution 5 times / without dilution comparison Etoprofos at mg/kg LC-QQQ-MS/MS > Tomato without dilution Tomato diluted 5 times

17 Dilution 5 times / without dilution comparison Dimethoate at mg/kg LC-QQQ-MS/MS > Orange without dilution Orange diluted 5 times 2.1

18 Dilution 5 times / without dilution comparison Pymetrozine at mg/kg LC-QQQ-MS/MS > Tomato without dilution Tomato diluted 5 times

19 Dilution 5 times / without dilution comparison Pymetrozine at mg/kg LC-QQQ-MS/MS > Orange without dilution Orange diluted 5 times 3.7

solvent) 140 120 100 80 60 40 undiluted 1:2 1:10 20 0 < 20% 20% - 40% 40% - 60% 60% - 80% 80% - 100% 100% -

20 Dilution of black tea extract Response (%) after dilution black tea spiked with 300 pesticides 1pg/µl recovery of different diluted extracts compared to solvent (calculated by comparing with the appropriate level in solvent) undiluted 1:2 1: < 20% 20% - 40% 40% - 60% 60% - 80% 80% - 100% 100% - 120% 120% - 150% 150% - 200% > 200% not found overall increase in recovery by dilution WEBEX triggered MRM October 23, 2012

Co-extracted matrix components of orange.

orange matrix compounds and Isoproturon Isoproturon

22 Co-extracted matrix components of orange. LC-TOF-MS 8017 Matrix compounds LC-TOF-MS XIC of orange matrix compounds and Isoproturon Isoproturon (100 µg kg -1 ) Co-extracted matrix components of tomato. LC-TOF-MS 2408 Matrix compounds LC-TOF-MS XIC of tomato matrix compounds and Isoproturon Isoproturon (100 µg kg -1 )

23 Co-extracted matrix components of orange. LC-TOF-MS No Dilution 8017 Matrix compounds Dilution 1/ Matrix compounds

24 Dilution 5 times / without dilution comparison Azoxystrobyn at mg/kg LC-QQQ-MS/MS > 344 Tomato diluted 5 times Pepper diluted 5 times Orange diluted 5 times Tomato without dilution Pepper without dilution Orange without dilution

25 Dilution 5 times / without dilution comparison Methomyl at mg/kg LC-QQQ-MS/MS > 106 Tomato diluted 5 times Pepper diluted 5 times Orange diluted 5 times Tomato without dilution Pepper without dilution Orange without dilution

26 Dilution 5 times / without dilution comparison Pirimicarb at mg/kg LC-QQQ-MS/MS > 72 Tomato diluted 5 times Pepper diluted 5 times Orange diluted 5 times Tomato without dilution Pepper without dilution Orange without dilution

27 Dilution 5 times / without dilution comparison Dimethoate at mg/kg LC-QQQ-MS/MS > 199 Tomato diluted 5 times Pepper diluted 5 times Orange diluted 5 times Tomato without dilution Pepper without dilution Orange without dilution

30 Introduction The main challenge is in obtaining an extract which contains the target analytes but possibly low amount of fat. The most common methods for fat removal from extract are: low temperature precipitation (freezing-out), gel permeation chromatography (GPC) adsorption (dispersive solid-phase extraction, solid-phase extraction). Avocado is an example of high oil commodity, it contains up to 30% of fat. The major components of avocado fat are fatty acids (oleic, palmitic and linoleic) and triglycerides.

31 Introduction Average composition of avocado: water (66.5%); lipids (23.5%); protein (1.9%); sugars (0.4%). QuEChERS Ethyl acetate method miniluke

32 Sorbent selection for QuEChERS Interaction of phospholipid molecule with empty d-orbital of zirconium atom.

33 Selected compounds # Compound tr (min) SRM1 CE1 (V) SRM2 CE2 (V) 1 Acephate 3, / / Acetamiprid 10, / / Aldicarb 13, / / , / / Azoxystrobin 20, / / Bitertanol 21, / / Boscalid 21, / / Bromuconazole 20,26 ; 21, / / Bupirimate 19, / / Buprofezin 24, / / Carbaryl 16, / / Carbendazim 3, / / Chlorantranilipr ole 18, / / Chlorpyriphos 28, / / , / / Clofentezine 24, / / Cymoxanil 12, / / Cyproconazole 19, / / Cyprodinil 18, / / Cyromazine 2, / / Diazinon 25, / / Dicrotophos 5, / / Diethofencarb 20, / / Difenoconazole 23, / / Dimethoate 10, / / Dimethoate-d6 10, / / Dimethomorph 18,56 ; 18, / / Diniconazole 22, / / Diphenylamine 22, / / Dodine 17, / / Ethion 28, / / Ethirimol 6, / / Ethoprophos 21, / / Fenamidone 21, / / Fenamiphos 20, / / Fenarimol 20, / / Fenazaquin 27, / / Fenbuconazole 21, / / Fenoxycarb 22, / / # Compound tr (min) SRM1 CE1 (V) SRM2 CE2 (V) 40 Fenpropathrin 28, / / Fenpropimorph 15, / / Fenpyroximate 27, / / Fenthion 24, / / Fluazifop 20, / / Flufenoxuron 27, / / Fluquinconazole 21, / / Flusilazole 21, / / Flutriafol 16, / / Formetanate 2, / / Haloxyfop 22, / / Hexythiazox 28, / / Imazalil 13, / / Imidacloprid 9, / / Indoxacarb 25, / / Iprovalicarb 20, / / Azinphosmethyl Chlorpyriphosmethyl Isofenphos- Methyl 25, / / Isoprocarb 18, / / Kresoxim-methyl 23, / / Linuron 20, / / Malathion 22, / / Malathion-d10 22, / / Mandipropamid 21, / / Metalaxyl 17, / / Metconazole 22, / / Methamidophos 3, / / Methidathion 20, / / Methiocarb 19, / / Methoxyfenozide 22, / / Metobromuron 18, / / Monocrotophos 4, / / Myclobutanil 20, / / Nitenpyram 4, / / Oxamyl 4, / / , / / Paclobutrazole 19, / / Pencycuron 25, / / Pendimethalin 27, / / Phenthoate 24, / / # Compound tr (min) SRM1 CE1 (V) SRM2 CE2 (V) 79 Phosalone 25, / / Phosmet 20, / / Phoxim 25, / / Pirimicarb 6, / / , / / Prochloraz 19, / / Profenofos 25, / / Propamocarb 3, / / Propiconazole 22, / / Propoxur 16, / / Propyzamide 21, / / Pymetrozine 2, / / Pyraclostrobin 24, / / Pyrethrins 29, / / Pyridaben 29, / / Pyrimethanil 15, / / Pyriproxyfen 27, / / Quinoxyfen 25, / / Rotenone 22, / / Spinosyn A 17, / / Spinosyn D 18, / / Spirodiclofen 30, / / Spiromesifen 30, / / Tebuconazole 21, / / Tebufenozide 23, / / Tebufenpyrad 26, / / Tetraconazole 21, / / Trichlorfon 8,30 257/ / Thiabendazole 3, / / Thiacloprid 12, / / Thiamethoxam 7, / / Oxydemetonmethyl Pirimiphosmethyl Thiophanatemethyl 15, / / TPP 24, / / Triadimefon 21, / / Triazophos 22, / / Triflumuron 23, / / Triticonazole 19, / / Zoxamide 24, / /

%B Agilent Zorbax Eclipse XDB C8 150 4.6mm; d.i.:5 µm Mobile phase: Acetonitrile and water with 0.1 % formic acid Gradient: 20% AcN isocratic mode for 3 min. Lineal increasing until el 100% in 27 min.

34 %B Agilent Zorbax Eclipse XDB C mm; d.i.:5 µm Mobile phase: Acetonitrile and water with 0.1 % formic acid Gradient: 20% AcN isocratic mode for 3 min. Lineal increasing until el 100% in 27 min. and 3 min. more on isocratic mode at 100% AcN Injection volumen: 10µl Flow: 0.6 ml/min Gradient Time (min) Agilent 6490 Triple Quadrupole Gas Temp 120ºC Gas Flow 15 l/min Nebulizer 35 psi Sheat Gas Temp 375ºC Sheat Gas Flow 12 l/min Capillary 3500 V Nozzle voltaje 300 V Source Ailent Jet Stream: ESI (+) and ESI (-) ifunnel Parameters High Pressure RF: 150 V Low Pressure RF: 60 V

35 Precentage of total number of pesticides Sorbent selection for QuEChERS QuEChERS recoveries with different clean-up sorbents 100% 90% 80% 70% 60% Percentage of pesticides with recoveries in the % range 50% 40% 34% 42% 47% 46% 30% 20% 10% 0% QuEChERS Z-Sep+ QuEChERS PSA/C18 QuEChERS SiO2 QuEChERS Z-Sep evaporation of 0.50 ml, reconstitution in 0,50 ml of H 2 O (10% AcN)

36 Precentage of total number of pesticides Sorbent selection for QuEChERS QuEChERS recoveries with different clean-up sorbents 100% Percentage of pesticides with recoveries in the % range 95% 90% 80% 70% 60% 54% 59% 68% 50% 40% 34% 42% 47% 46% 30% 20% 10% 0% QuEChERS Z-Sep+ QuEChERS PSA/C18 QuEChERS SiO2 QuEChERS Z-Sep evaporation of 0.50 ml, reconstitution in 0,50 ml of H 2 O (10% AcN) evaporation of 0.50 ml, reconstitution in 1 ml of H 2 O (30% AcN)

37 Sorbent selection for QuEChERS Z-Sep provides cleaner QuEChERS extracts than combination of PSA and C18 Z-Sep PSA/C18

38 Co-extracted matrix components of avocado with Z-Sep and PSA+C18. LC-TOF/MS Absolute height counts Z-Sep PSA+C18 PSA+C matrix compounds Z-Sep 2807 matrix compounds Mass Profiler Professional Agilent Tech.

39 miniluke method 10 g of sample Mix 30s by Polytron Add 20mL of Acetone Add 30 g Na 2 SO 4 and 40 ml Petroleum ether: Dichloromethane (1:1) Mix 30s by Polytron Addition of Dilution Internal Standard-Linuron-d6 Addition of Injection Internal Standard-Dimethoate-d6 Centrifugue 5 min. at 3500 r.p.m. Analysis Evaporate to dryness and reconstitute

40 Ethyl acetate multiresidue method 10 g of sample Shake 3 s by hand Add 10 ml ethyl acetate and 1.5 g of NaCl and 8 g of MgSO 4 Shake automatically in Agitax 15 min Addition of Dilution Internal Standard Linuron-d6 Centrifugue 5 min r.p.m. Analysis Addition of Injection Internal Standard-Dimethoate-d6

41 Modified QuEChERS method 10 g of sample Shake automatically in Agitax Shake automatically in Agitax Add 4 g MgSO g NaCl + 1 g NaCitrate 2 H 2 O g NaCitrate 3/2 H 2 O Centrifugue 5 min r.p.m. Clean-up with 175 mg Z-Sep mg MgSO 4 Add 10 ml acetonitrile Take 5 ml aliquot Shake 30 second in Vortex Addition of Dilution Internal Standard-Linuron-d6 Addition of Injection Internal Standard-Dimethoate-d6 Centrifugue 5 min r.p.m. Evaporate to dryness and reconstitute Analysis

42 Comparison of different MRMs Fat content in the final avocado extracts Ethyl acetate method miniluke QuEChERS with Z-Sep

43 Precentage of total number of pesticides Comparison of different MRMs 100% Percentage of pesticides with recoveries in the % range 96% 90% 80% 70% 60% 54% 62% 70% 76% 50% 40% 42% 30% 20% 10% 0% MiniLuke Z+ Fat 150 mg silica EtAc Z-Sep miniluke QuEChERS Z-Sep+ QuEChERS PSA/C18 QuEChERS 150 mg silica Ethyl acetate QuEChERS Z-Sep

44 Matrix effects Suppression in particular extracts of avocado 57% 18% 26% 0%-20% 21%-50% 51%< 30% 0%-20% 26% 44% 21%-50% 51%< Ethyl acetate method miniluke 39% 17% 44% 0%-20% 21%-50% 51%< QuEChERS with Z-Sep

45 Matrix effects Calibration plots of diazinon (304.6 > 169.0) 4.E+08 3.E+08 3.E+08 Solvent QuEChERS with Z-Sep miniluke Ethyl acetate 2.E+08 2.E+08 1.E+08 5.E+07 0.E Concentration [ppb]

46 Matrix effects Calibration plots of cyprodinil (225.8 > 93.0) 1.E+08 1.E+08 1.E+08 Solvent QuEChERS with Z-Sep miniluke Ethyl acetate 8.E+07 6.E+07 4.E+07 2.E+07 0.E Concentration [ppb]

47 Matrix effects Calibration plots of pymetrozine (118.1 > 105.0) 3.E+07 2.E+07 2.E+07 Solvent QuEChERS with Z-Sep miniluke Ethyl acetate 1.E+07 5.E+06 0.E Concentration [ppb]

48 Validation of QuEChERS with Z-Sep in avocado and almond

7 H 2 O Sample amount: 5 g Water addition: 5 ml Evaporation:

49 Validation Sample amount: 10 g Water addition: no Evaporation: 0.5 ml Reconstitution: 0.3 ml ACN H 2 O Sample amount: 5 g Water addition: 5 ml Evaporation: 0.5 ml Reconstitution: 0.4 ml ACN H 2 O Dilution factor: 2 Dilution factor: 4

50 Percentage of total number of pesticides Validation Recoveries 50% 50% 40% 40% 30% 20% 10% 30% 20% 10% Avocado Almond 0% 0% mg/kg mg/kg Percentage of pesticides with recoveries in the % range: -avocado 95% -almond 81% Percentage of pesticides with recoveries in the % range: -avocado 95% -almond 83%

51 Recovery Validation 120% 120% 70% 70% 0% % Water solubility [mg/l] Water solubility [mg/l] Almond, fat content around 50% Avocado, fat content up to 30%

52 Percentage of total number of pesticides LOQs Avocado Almond mg/kg mg/kg > mg/kg

53 Percentage of total number of pesticides Validation Relative standard deviation (n = 5) 100% 100% 80% 80% 60% 40% 60% 40% Avocado Almond 20% 20% 0% 0% 1-5% 6-10% 11-15% 16-20% 1-5% 6-10% 11-15% 16-20% mg/kg mg/kg

54 Percentage of total number of pesticides Validation Linearity Matrix effects 90% 80% 70% 60% 90% 80% 70% 60% 50% 40% 30% 20% 50% 40% 30% 20% Avocado Almond 10% 10% 0% Linearity range (ppb) 0% < 20% 21% - 50% 50% < Suppression

55 Recovery 120% 110% 100% 90% 80% Malathion-d10 TPP 70% 60% Days

56 STD 14% 12% 10% 8% 6% 4% Dimethoate-d6 Malathion-d10 TPP 2% 0% Days

57 Collaborative study with tea

58 Co-extracted matrix components of green tea. LC-TOF-MS Absolute height counts AOAC method 2641 compounds Modified QuEChERs with CaCl compounds Mass Profiler Professional Agilent Tech.

Tea Extraction method 5g tea sample Add 15 ml acetonitrile Homogenize at 13500r/min for 1min, and centrifuge at

combined and then concentrated to about 1 ml Cleanert TPT Cartridge (2g, 12mL) or Envi-Carb/PSA(0.5g/0.

5 ml Add IS Heptachlor epoxide for GC/MS-SIM, GC/MS/MS or Chlorpyrifos-methyl for LC-MS/MS.

59 Tea Extraction method 5g tea sample Add 15 ml acetonitrile Homogenize at 13500r/min for 1min, and centrifuge at 4200r/min for 5 min before the supernatants are taken, while the dregs are re-extracted and the extracts are combined and then concentrated to about 1 ml Cleanert TPT Cartridge (2g, 12mL) or Envi-Carb/PSA(0.5g/0.5g,6mL) for clean up. Elute with 25 ml acetonitrile + toluene (3+1), concentrated to about 0.5 ml Add IS Heptachlor epoxide for GC/MS-SIM, GC/MS/MS or Chlorpyrifos-methyl for LC-MS/MS. Evaporate to dryness with nitrogen at 35º Make up to 1.5 ml with hexane Make up to 1.5 ml with acetonitrile + water (3+2) GC/MS Determination 3.33 times concentration LC-MS/MS Determination

60 Chromatographic and acquisition parameters HPLC Parameters Brand name(type) Agilent (1200) Column temperature 40ºC Mobile phase A: H2O 0.1% formic acid - B: AcN Flow rate(ml/min) 0.4 Gradient program (min-mobile phase A%) Min - A(%) Injection volume 10 µl Brand name(type) Agilent Zorbax C8 Specification 5um, 4.6x150 mm MS/MS Parameters Brand name(type) Agilent QQQ 6490 Source mode ESI Ion spray voltage 3500 Dry gas temperature 120ºC Dry gas flow rate 15 l/min Sheat Gas gas temperature 375ºC Sheat gas pressure 12 l/min Compounds Quantifying transition Qualifying transition Chlorpyrifos-methyl 321.9> >289.9 Acetochlor 270.2> >148.1 Benalaxyl 326> >294 Bensulide 398.1> >314.5 Butralin 296.2> >222.2 Chlorpyriphos 349.9> >97 Clomazone 240.1> >89 Diazinon 305> >153 Ethoprophos 243.1> >214.9 Flutolanil 324.1> >282.2 Imidacloprid 256> >175 Indoxacarb 528.1> >150 Kresoxim methyl 314.1> >206 Monolinuron 215> >148.1 Picoxystrobin 368.1> >145 Pirimiphos-methyl 306.2> >108.2 Propoxur 210.1> >168.1 Quinoxyfen 308.1> >271.9 Tebufenpyrad 334.2> >147.1 Triadimefon 294.2> >69.1 Trifloxystrobin 409.2> >206.2

61 TIC Green Tea Spiked with 50uL working solution + Chlorpyrifos methyl TIC Oolong Tea Spiked with 50uL working solution + Chlorpyrifos methyl

Green tea validation data No. Pesticides Retention time/ min Calibration curve Spike levels (µg/kg) R 2 Slope Intercept Recovery/% RSD/% IS Chlorpyrifos-methyl 17.8 1.9 1 Acetochlor 14.8 0.9996 1253.

62 Green tea validation data No. Pesticides Retention time/ min Calibration curve Spike levels (µg/kg) R 2 Slope Intercept Recovery/% RSD/% IS Chlorpyrifos-methyl Acetochlor Benalaxyl Bensulide Butralin Chlorpyriphos Clomazone Diazinon Ethoprophos Flutolanil Imidacloprid Indoxacarb Kresoxim methyl Monolinuron Picoxystrobin Pirimiphos-methyl Propoxur Quinoxyfen Tebufenpyrad Triadimefon Trifloxystrobin

63 Oolong tea validation data No. Pesticides Retention time/ min Calibration curve Spike levels (µg/kg) R 2 Slope Intercept Recovery/% RSD/% IS Chlorpyrifos-methyl Acetochlor Benalaxyl Bensulide Butralin Chlorpyriphos Clomazone Diazinon Ethoprophos Flutolanil Imidacloprid Indoxacarb Kresoxim methyl Monolinuron Picoxystrobin Pirimiphos-methyl Propoxur Quinoxyfen Tebufenpyrad Triadimefon Trifloxystrobin

64 Samples Butralin in green tea at mg/kg Chlorpyrifos in oolong tea at mg/kg

65 Pooling Samples

66 Pooling samples (Tomato, Cucumber and Lettuce) Azinphos-methyl mg/kg in Tomato LC-QQQ-MS/MS > ppb Tomato without dilution Pooling samples (Ratio 1:1:1) Pooling samples (Ratio 1:1:1 diluted 5 times) 3.3 ppb 0.8 ppb

67 Pooling samples (Tomato, Cucumber and Lettuce) Methomyl mg/kg in Tomato LC-QQQ-MS/MS > ppb Tomato without dilution Pooling samples (Ratio 1:1:1) Pooling samples (Ratio 1:1:1 diluted 5 times) 3.4 ppb 0.7 ppb

68 Co-extracted matrix components. LC-TOF-MS Absolute height counts Cucumber 4559 compounds Lettuce 3718 compounds Tomato 3775 compounds Cucumber+Lettuce+Tomato 4462 Matrix compounds

Validation Report 21

EURL for Cereals and Feeding stuff National Food Institute Technical University of Denmark Validation Report 21 Determination of pesticide residues in maize for livestock feed by GC-MS/MS and LC-MS/MS