Effects of high pco2 on early life development of pelagic spawning marine fish

Transcription

1 Marine and Freshwater Research, 2017, 68, CSIRO 2017 Supplementary material Effects of high pco2 on early life development of pelagic spawning marine fish Ana M. Faria A,E,1, Soraia Filipe B,1, Ana F. Lopes A, Ana P. Oliveira C, Emanuel J. Gonçalves A and Laura Ribeiro D A Marine and Environmental Sciences Centre (MARE), ISPA Instituto Universitário, Rua Jardim do Tabaco, 34, PT Lisboa, Portugal. B Superior School of Tourism and Sea Technology, Santuário de Nossa Senhora dos Remédios, PT Peniche, Portugal. C Instituto Português do Mar e da Atmosfera (IPMA, IP), Division of Oceanography and Environmental Bioprospecting, Avenida Brasília, 6, PT-1200 Lisboa, Portugal. D Instituto Português do Mar e da Atmosfera (IPMA, IP), Aquaculture Research Station, Avenida 5 de Outubro, PT Olhão, Portugal. E Corresponding author. address: afaria@ispa.pt Page 1 of 8

2 Table S1. Generalised linear mixed-effects (GLME) analysis for multiple descriptive parameters of Solea senegalensis, Diplodus sargus and Argyrosomus regius eggs and larvae, from two batches (A and B), maintained at three experimental pco 2 conditions (control, medium and high) P-values in bold indicate statistically significant differences to control pco 2 condition and Batch A (P < 0.01) Solea senegalensis Hatching rate Intercept Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Survival rate Intercept Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Growth rate Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Dry weight Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Egg diameter Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Diplodus sargus Hatching rate Intercept Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Survival rate Intercept Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Page 2 of 8

3 Growth rate Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Dry weight Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Egg diameter Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Argyrosomus regius Hatching rate Intercept Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Survival rate Intercept Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Growth rate Intercept Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Dry weight Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Egg diameter Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Page 3 of 8

4 Table S2. Generalised linear mixed-effects (GLME) analysis for morphometric traits of Solea senegalensis, Diplodus sargus and Argyrosomus regius larvae at 0 days post-hatching, from two batches (A and B), maintained at three experimental pco 2 conditions (control, medium and high) P-values in bold indicate statistically significant differences to control pco 2 condition and Batch A (P < 0.05) Solea senegalensis Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Yolk sac area Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Diplodus sargus Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Yolk sac area Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Argyrosomus regius Intercept < Treatment Medium < Treatment High Batch B < Batch B Treatment Medium Batch B Treatment High Page 4 of 8

5 Yolk sac area Intercept < Treatment Medium < Treatment High Batch B < Batch B Treatment Medium Batch B Treatment High Intercept < Treatment Medium < Treatment High Batch B < Batch B Treatment Medium < Batch B Treatment High Page 5 of 8

6 Table S3. Generalised linear mixed-effects (GLME) analysis for morphometric traits of Solea senegalensis, Diplodus sargus and Argyrosomus regius larvae at 3 days post-hatching, from two batches (A and B), maintained at three experimental pco 2 conditions (control, medium and high) P-values in bold indicate statistically significant differences to control pco 2 condition and Batch A (P < 0.05) Solea senegalensis Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Diplodus sargus Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Argyrosomus regius Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Page 6 of 8

7 Table S4. Generalised linear mixed-effects (GLME) analysis for otolith morphometrics of Solea senegalensis, Diplodus sargus and Argyrosomus regius larvae at 3 days post-hatching, from two batches (A and B), maintained at three experimental pco 2 conditions (control, medium and high) P-values in bold indicate statistically significant differences to control pco 2 condition and Batch A (P < 0.05) Solea senegalensis Area Intercept < Treatment Medium Treatment High Batch B < Batch B Treatment Medium Batch B Treatment High Perimeter Intercept < Treatment Medium Treatment High Batch B < Batch B Treatment Medium Batch B Treatment High Width Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Diplodus sargus Area Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Perimeter Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Width Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Argyrosomus regius Area Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Page 7 of 8

8 Perimeter Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Width Intercept < Treatment Medium Treatment High Batch B Batch B Treatment Medium Batch B Treatment High Page 8 of 8