sensitive simple small From the Leader in Evaporative Light Scattering Detection Grace Davison Discovery Sciences Brochure #531 GEN

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1 sensitive Most sensitive ELSD available simple Easiest to use and maintain small Integrates easily with any HPLC Grace Davison Discovery Sciences Brochure #5 From the Leader in Evaporative Light Scattering Detection GEN Copyright 006 Alltech Associates, Inc.

2 Introduction To ELSD Leading the Way in ELSD Technology for More than 0 Years No one has more experience designing and manufacturing ELSDs than Alltech, now a part of Grace. Our customers' needs continue to drive the evolution of our ELSD design and development programs. 985 MK II 988 MK IIA 99 MK III 994 MK IIIA 996 ELSD ELSD LTA 000 ELSD ELSD ELSD 000ES ELSD* *patent pending ELSD Advantages Universal Have confidence you re seeing everything in your sample Improve quantitative measurements Estimate purity more accurately Sensitive Detection limits in the low nanogram range Gradient Compatible Maintain stable baselines during steep gradients Alltech ELSDs simplify analysis of difficult compounds: pharmaceuticals impurities phospholipids fatty acids surfactants nutraceuticals excipients amino acids triglycerides polymers carbohydrates ELSD Detection Process:. Nebulization Column effluent passes through a needle and mixes with nitrogen gas to form a dispersion of droplets.. Evaporation Droplets pass through a heated drift tube where the mobile phase evaporates, leaving a fine mist of dried sample particles in solvent vapor.. Detection The sample particles pass through a cell and scatter light from a laser beam. The scattered light is detected, generating a signal. Copyright 006 Alltech Associates, Inc.

3 ELSD Advantages ELSD Advantages Over Other LC Detectors ELSD RI UV MS CHART KEY Sensitivity Gradient Capability Baseline Stability Solvent Interference Mass Balance Excellent: Good: Poor: ELSD improves baseline stability and detection sensitivity compared to refractive index (RI) ELSD shows what may be missing from your UV chromatogram Solvent Front Sugar Alcohols. Xylitol (00ppm). Sorbitol (00ppm). Dulcitol (00ppm) Plant Oil Extract ELSD ELSD RI Min. UV at 54nm Min. Column: Alltech Prevail Carbohydrate ES, 5µm, 50 x 4.6mm, (Part No. 50) Mobile Phase: Acetonitrile:Water (79:) Flow Rate:.0 ml/min Column Temp: 0 C Use ELSD in parallel with UV/Vis and MS for maximum structural and concentration information Column: Alltech Alltima C8, μm, 50 x.mm, (Part No. 485) Mobile Phase: A: 0.% Formic Acid B: Methanol Gradient: Time: 0 40 %B: Flow Rate: 0.5 ml/min Column Temp: 0 C ELSD obtains a more accurate representation of sample mass than UV/Vis Steroid Hormone Formulation. Povidone (8 µg). Chlormadinone Acetate (.5 µg). Mestranol ( µg) Min. ELSD MS UV at 54nm Mephobarbital (500ppm). Secobarbital (500ppm) : Mixture Min. ELSD UV at 0nm Column: Vydac Denali 8DE C8, µm, 50 x.mm, (Part No. 8DE05) Mobile Phase: 0.% Formic Acid in Acetonitrile:Water (55:45) Flow Rate: 0. ml/min Column: Alltech Alltima HP C8 HL, 5µm, 50 x 4.6mm, (Part No ) Mobile Phase: Acetonitrile:Water (0:70) Flow Rate:.0 ml/min Column Temp: 45 C Copyright 006 Alltech Associates, Inc.

4 Sensitive & Reproducible With reduced noise and greater baseline stability, the 00 ELSD is more sensitive than any other ELSD available. Methyl Paraben (00ng). Propyl Paraben (00ng). Butyl Paraben (00ng) Competitor S Competitor P Competitor W Butyl Paraben Model Limit of Detection (LOD) 00 ELSD 5ng Competitor S Competitor P Competitor W Competitor X 00ng 00ng 50ng 50ng Competitor X 00 ELSD Column: Alltech Alltima C8, 5µm, 50 x 4.6mm, (Part No ) Mobile Phase: Water:Acetonitrile (40:60) Flow Rate:.0 ml/min Min. The 00 ELSD's near ambient temperature evaporation maximizes sensitivity for semi-volatiles The 00 ELSD is very reproducible as demonstrated by 0 identical injections of four ginkgolide components. Ibuprofen (00ppm) Component % RSD. Bilobalide.7. Ginkgolide C.. Ginkgolide A.6 4. Ginkgolide B.0 0 Min. 0 C.5 C 5 C 7.5 C 40 C 4.5 C Column: Alltech Alltima HP C8, µm, 00 x.mm, (Part No ) Mobile Phase: Acetonitrile: 0.%TFA in Water (80:0) Flow Rate: 0. ml/min Min. Column: Alltech Platinum C8, 5µm, 50 x 4.6mm, (Part No. 064) Mobile Phase: Water:Methanol:THF (65:5:0) Flow Rate:.0 ml/min 4 Copyright 006 Alltech Associates, Inc.

5 Easy To Use A Windows -based user interface makes navigating through menus intuitive and easy. All critical operating parameters and a scrolling chromatogram trace are shown on the large, full color LCD. A simple, step-by-step method wizard guides you through detector setup Select from seven different languages for the user interface: English Español Francais Deutsch Italiano Japanese Chinese An informative help function is available in each screen Additional Features: Control and collect data digitally through Agilent ChemStation and EZchrom chromatography data software Ensure GLP compliance with password protected screen and error log Store up to 00 methods for fast set up Save gas and extend optics lifetime by programming unit to enter stand by mode at end of run coming soon Copyright 006 Alltech Associates, Inc.

6 Easy To Maintain Maintenance Made Simple! Parts requiring cleaning or service are accessible from the front Remove the nebulizer with a simple twist and pull Clean the drift tube easily with the included custom brush Onboard diagnostics automate the troubleshooting process Full IQ/OQ/PQ Protocols Included with each ELSD We make it easy for you to integrate the ELSD into a qualified HPLC system. The 00 ELSD is thoroughly tested before shipment and calibration and performance documents are included with each instrument. Detailed IQ/OQ/PQ procedures are included with the detector to help you perform your own qualification. We also provide the option of onsite qualification performed by one of our service engineers. Copyright 006 Alltech Associates, Inc.

7 Applications Stable Baselines with Fast, Steep Gradients 0 Min.. Acetylsalicylic Acid. Diphenhydramine. Trimipramine 0658 Column: Alltech Alltima HP C8, µm, x 7mm, (Part No. 8767) Mobile Phase: A: 0.%TFA in Water B: 0.% TFA in Acetonitrile Gradient: Time: 0 %B: 0 90 Flow Rate:.5 ml/min Column Temp: 0 C Simplify Detection of Weak Absorbing Polymers. mpeg-sba 066 Use ELSD to Trigger Collection of UV Transparent Compounds in Prep Applications ,8 9, Min. Column: Vydac Denali 8DE C8, 0µm, 50 x mm, (Part No. 8DE05) Mobile Phase: A: Acetonitrile B: Dichloromethane Gradient: Time: %B: Flow Rate: 0 ml/min Column Temp: 5 C. LLLn. LLL. LLO 4. PLL 5. LOO 6. PLO 7. PLP 8. OOO 9. SLO 0. POO 0679 Easily Detect Non-Chromophoric Drug Compounds Spectinomycin Min. Column: Alltech ProSphere 00, 5µm, 50 x 4.6mm, (Part No. 5560) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 5 %B: Flow Rate:.0 ml/min Detect Difficult Samples without Derivatizing Biodiesel Derived from Soy Bean Oil Min Mono-acylglycerol (MAG). MAG. MAG 4. Fatty Acid Methyl Ester (FAME) 5. MAG 6. FAME 7. FAME 8. MAG 9. FAME 0. Di-acylglycerol (DAG). Tri-acylglycerol (TAG) Column: Alltech Alltima HP C8 HiLoad, 5µm, 50 x 4.6mm, (Part No ) Mobile Phase: A: Acetonitrile B: Dichloromethane Gradient: Time: %B: Flow Rate:.0 ml/min Min. Column: Alltech Alltima HP C8 Amide, µm, 50 x 4.6mm, (Part No. 8789) Mobile Phase: 0mM Ammonium Carbonate:Acetonitrile (98:) Flow Rate:.0 ml/min Improve Sensitivity for Carbohydrates Robitussin Flu Syrup Min. Column: Alltech Prevail Carbohydrate ES, 5µm, 50 x 4.6mm, (Part No. 50) Mobile Phase: A: Acetonitrile B: Water Gradient: Time: %B: Flow Rate:.0 ml/min Sucralose. Fructose. Sorbitol 4. Glucose 5. Sucrose 6. Maltose Copyright 006 Alltech Associates, Inc.

8 Ordering Information Description Alltech 00 ELSD* Part No. 00 ELSD 0V 40V 5584 *Patent Pending Description Services for the Alltech 00 ELSD Installation and Training Qualification IQ/OQ at Customer Site PQ at Customer Site IQ/OQ/PQ at Customer Site Part No. INSTALL Q000 Q000 Q000 Our dedicated applications chemists and technical support professionals are constantly adding new applications to our vast technical library. Visit to search and download applications or one of our applications brochures. Lipids Carbohydrates Pharmaceuticals Alltech 00 ELSD Specifications Light Source: Laser diode with collimating optics, 650nm, 0mW output, class IIIB Detector Element: Silicon photodiode Temperature Range: Ambient to 0ºC in ºC increments Nebulizer Gas: Nitrogen preferred; Calibrated to 4.0L/ min Inlet Pressure: 60 80psig Typical Operating Range:.0.0L/min Flow Control: Digital Mass Flow Control Mobile Phase Flow Rate: To.0mL/min Analog Output: V or 0mV full scale Communications: Inputs: TTL/contact closure for auto zero, gas shutoff, start, and standby Outputs: Contact Closure for pump shutdown in error conditions, general events Data Ports: USB, RS, Ethernet User Interface: Full color Microsoft Windows based Graphical LCD with alphanumeric keypad Power Requirements: 0/40V, 50/60Hz Dimensions:.6" H x 0." W x 9.5" D (9.5cm H x 6.cm W x 49.5cm D) Weight: 0lbs (6Kg) IEC/FDA Classification: Class Laser Product; Complies with CFR Subpart J Manufacturing Standards: Meets all applicable safety and EMC certifications; CE, UL, and CSA certified Warranty: year parts and labor See how the 00 ELSD can benefit your analysis! Visit and click on "Contacts". Ask about sample analysis or a product demonstration in your lab. ALLTECH, ALLTIMA, DENALI, FLEXIT, GROM, JONES CHROMATOGRAPHY, MODCOL, PLATINUM, PREVAIL, PROSPHERE, and VYDAC are trademarks, registered in the United States and/or other countries, of Alltech Associates, Inc. DAVISIL, GRACE and GRACE DAVISON DISCOVERY SCIENCES are trademarks, registered in the United States and/or other countries, of W. R. Grace & Co.-Conn. MICROSOFT WINDOWS and WINDOWS are trademarks, registered in the United States and/or other countries, of Microsoft Corporation. ROBITUSSIN is a trademark, registered in the United States and/or other countries, of American Home Products Corp. This trademark list has been compiled using available published information as of the publication date of this brochure and may not accurately reflect current trademark ownership. Grace Davison Discovery Sciences is a product group of W. R. Grace & Co.-Conn. Alltech Associates, Inc. is a wholly owned subsidiary of W. R. Grace & Co.-Conn. Copyright 006 Alltech Associates, Inc. All rights reserved. The information presented herein is derived from our testing and experience. It is offered, free of charge, for your consideration, investigation and verification. Since operating conditions vary significantly, and since they are not under our control, we disclaim any and all warranties on the results which might be obtained from the use of our products. You should make no assumption that all safety or environmental protection measures are indicated or that other measures may not be required. This product(s) may be covered by patents or patents pending. Grace reserves the right to change prices and/or specifications without prior notification. Printed in the USA. Grace Davison Discovery Sciences Headquarters 05 Waukegan Road Deerfield, Illinois Tel: Fax: contact.alltech@grace.com Web: 09/06 #5 Grace Davison Discovery Sciences Copyright 006 Alltech Associates, Inc.