221 and 222 XL Liquid Handlers User s Guide

Transcription

1 221 and 222 XL Liquid Handlers User s Guide

2 Errata 221/222 XL User s Guide page Appendix A-2 Model 221XL Model 222XL Part Number Qty Part Number Qty Description Constricting needle, 316L,160.5 x 1.5 x 1.1 mm... page Appendix A-13 Model 221XL Model 222XL Part Number Part Number Description Constricting needle 316L, x 1.5 x 1.1 mm... Ref : LT801483/A

3 221 and 222 XL Liquid Handlers User s Guide LT801205/H Gilson SAS All rights reserved April 2003

4

5 Contents Table of Contents 0 Safety 1 Introduction 2 Description Unpacking Warranty Customer Service Mechanical Assembly Pumps Electrical Assembly Electrical Control Socket Microswitch Settings Rinsing Stations Installing a Liquid Handler Power Connection/Fuses Voltage Selection Fuses Connecting the Power Cord Installing the Vertical Arm Needle/Liquid Level Detector Principle of the Liquid Level Detector Sensitivity Limits Installation Gilson Sampler Controller Keypad Pumps Syringe Pump Dilutor Minipuls 3 Peristaltic Pump Installing The Additional Accessories Heightening Kit Metallic Front Tray Assembly Installation Levelling the Tray Platform Vertical Arms Needles Constricting Needles Teflon Needles Double Needles Table of Contents-1

6 TransferPorts Installation Injection Ports Installation Thermostated Racks Installation Perforated Covers for Thermostated Racks Universal Rack Free Racks Solvent Racks Needle Wiper Low Pressure Valve Maintenance Appendices Cleaning Lubrication Transportation Position Alignment Check A - Parts and Accessories Lists... Appendix A-1 Standard Equipment... Appendix A-2 Accessories... Appendix A-3 Gilson Sample Racks... Appendix A-4 FEP Tubing... Appendix A-11 Parts Lists for 221 and 222 XL Generics... Appendix A-12 B - GSIOC... Appendix B-1 Immediate Commands... Appendix B-2 Buffered Commands... Appendix B-5 C - Technical Data...Appendix C-1 Hydraulics...Appendix C-2 Mechanics...Appendix C-3 Electrical...Appendix C-4 Control...Appendix C-5 Programs...Appendix C-6 Environmental Conditions...Appendix C-7 D - Reference Information... Appendix D-1 Chemical Inertness of Plastic Used... Appendix D-2 Rack Compatibility Table... Appendix D-3 Table of Contents-2

7 Safety 0 Safety Read this section carefully before installing and operating the instrument. For safe and correct use of the instrument, it is essential that both operating and service personnel follow generally accepted safety procedures as well as the safety instructions given in this document, the 221 and 222 XL Liquid Handlers User s Guide. The instruments described in this document are liquid handlers and should be used for automatic liquid transfer operations only. They should only be used in the laboratory or similar indoor environment for analytical purposes, by qualified personnel. If an instrument is used in a manner not specified by Gilson, the protection provided by the instrument may be impaired. When the vertical arm and needle have been installed, it is necessary to check that nothing hinders the movement of the instrument parts (i.e. needle and arms). Nothing should be placed in or around the field of movement of either the horizontal or vertical arms. Due to the fact that the needle installed on the vertical arm may contain a dangerous substance, you are strongly advised against interfering in the work area of the instrument until the instrument has come to a complete stop. Voltages present inside the instrument are potentially dangerous. If there is a problem with the instrument, the power cable should be removed until qualified service personnel have repaired it. This is to prevent anyone from inadvertently using the instrument, thus causing possible harm to themselves, or damage to the instrument itself. The leakage current of this instrument is within the limits allowed by international safety standards for laboratory equipment. An efficient ground connection is imperative for the physical protection of the user. Power supply cord reference is for use in France and Germany. Power supply cord reference is for use in USA and Canada. For other countries contact your local Gilson distributor. You must only use the type of fuse described in this document: 0.5 Amp type T slow blow. Safety-1

8 Safety Symbol ~ I O! Explanation Alternating current PROTECTIVE CONDUCTOR TERMINAL On (Supply switch) Off (Supply switch) Caution, risk of electric shock Caution (refer to User s Guide) Adequate protection including clothing and ventilation must be provided if dangerous liquids are used in the analytical work. In case of incidental spillage, carefully wipe with a dry cloth, taking into account the nature of the spilled liquid and the necessary safety precautions. Cleaning, dismantling, maintenance, adjustment and repair should only be performed by personnel trained in such work, and who are aware of the possible dangers involved. These instruments must not be sterilized, using an autoclave, or any other mechanical device. When you need to clean an instrument, use one of the three following methods: 1 - a clean dry cloth, 2 - a cloth dampened with water, 3 - a cloth dampened with soapy water. If a cloth dampened with soapy water is used to clean the instrument, only domestic soap may be used. No other form of detergent or chemical may be used. These electronic and hazard symbols appear on the 221/222: Safety-2

9 Introduction 1 Unpacking...2 Warranty...3 Customer Service...4 This manual is intended for users of model 221 and 222 liquid handlers. Only procedures described in this manual should be carried out by the user. 221 and 222 liquid handlers are designed to work in a GSIOC environment. GSIOC (Gilson Serial Input Output Channel) is a communications channel which links various Gilson modules together in a system. Liquid handlers are slave XYZ robots which can be controlled either by computer or the Gilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations and sample preparation. They can be configured as stand alone preparation devices, easy to use sample transfer instruments or as systems for complete automation of sample preparation and transfer. 1-1

10 Introduction 1 Unpacking Unpacking Liquid handlers are delivered in a single box containing both the instrument and its standard parts (e.g. needles, tubing, etc...). The original box and packing assembly should be kept in case the equipment has to be returned to the factory and especially while the instrument is under warranty. All parts contained in the box must be checked off by the user against the packing list supplied. This should be performed as soon as possible even if the instrument is not to be put to immediate use. The supplier should be notified immediately of any inconsistencies between the box contents and the packing list and/or of damage to parts. When unpacking your instrument: Remove and open the upper compartment containing the standard parts (cables, needles, etc.), Slide the unit and packing assembly out of the box, Remove the locking screw, Remove packing assembly. Horizontal arm Arm-locking screw When delivered, the horizontal arm of the liquid handler is fixed in its transportation position by means of a screw. This screw must be removed before the unit is installed. It is located behind a plastic plug situated on the right-hand side-panel (refer to the opposite figure). The screw should be replaced whenever the liquid handler is transported, as failure to do so could result in severe mechanical damage. If this screw is lost, a replacement screw has been provided and is situated at the top lefthand corner of the rear of the unit. Plastic plug 1-2

11 Introduction 1 Warranty If the instrument does not appear to function correctly, first verify the electrical connections are correct and that the instrument is switched ON. Warranty Contact your Gilson distributor for technical advice or an eventual service visit. Any service required will be given within the warranty conditions assured by your Gilson distributor. 1-3

12 Introduction 1 Customer Service Customer Service Gilson and its worldwide network of authorized representatives provide you with four types of assistance: sales, technical, applications and service. The service personnel of Gilson's representatives are able to serve you more efficiently if you provide the following information: 1) The serial number and model number of the equipment involved. 2) Computer model (if used), available memory, microprocessor and operating software version(s) in operation. 3) The installation procedure you used. 4) A concise list of the symptoms. 5) A list of operating procedures and conditions you were using when the problem arose. 6) A list of other devices connected to the system and a system diagram showing the connections. 7) A list of other electrical connections in the room. 1-4

13 Description 2 This chapter describes the different mechanical, electrical and hydraulic components of the liquid handlers. 2-1

14 Description 2 Mechanical Assembly Mechanical Assembly Figures show the 221 and 222 in their standard configuration. To see which racks are compatible with the different possible configurations, refer to the table on the last page of this User's Guide. Vertical arm Needle holder Liquid level detector cable Horizontal arm Carriage casting Needle guide Rinsing station Support bar Tray holding bar 2-2

15 Description 2 Mechanical Assembly Rinsing station Support bar Tray rail Hole containing spare arm-securing screw Power socket 6-pin FCC type RS232 socket for sampler controller keypad Input/output sockets Microswitches GSIOC connector 25-pin D-type RS232 socket 2-3

16 Description 2 Pumps 401 Dilutor Pumps The 221 and 222 units can be used with a 402 Syringe Pump, 401 Dilutor or Minipuls 3 peristaltic pump connected to the needle. Single Syringe and Valve Configuration All three pumps are shown in the following diagrams. The pump outlet should be connected to the needle via the spiral transfer tubing (described in the installation section). The Minipuls 3 cannot be used to aspirate from a vial. Dual Syringe and Tee Junction Configuration Minipuls 3 2-4

17 Description 2 Electrical Assembly The rear panel of the liquid handler includes: 1. One sector block containing the power socket, fuse holder and ON/OFF switch. 2. One 6-pin FCC type socket to which is connected the sampler controller keypad via the RS232 coiled cable. Electrical Assembly 3. Four electrical control sockets- four inputs, seven outputs (including three relay type) and one analog input, one 12 V DC output and four ground connections. 4. Eight microswitches which serve to identify the machine and to activate certain functions such as changing the baud rate, local clock, etc. 5. One GSIOC connector through which the 402 Syringe Pump, 401 Dilutor or Minipuls 3 peristaltic pump is connected. 6. One 25-Pin D-type RS232 socket for computer connection. Never connect a liquid handler to both a sampler controller keypad and a computer simultaneously. 2-5

18 Description 2 Electrical Assembly Electrical Control Socket Located at the rear of the unit are four electrical control sockets which consist of four contact inputs and seven outputs (including three relay type outputs). They also consist of one analog input which can be configured to serve as a fifth contact input (refer to Microswitch Settings ). The signal voltage of the analog input is + or - 100mV. Use any of the cables supplied in the cable kit for electrical connections (ref ) to coordinate any peripheral instruments with the liquid handler. Four barrier strip connectors are provided for this purpose. no nc c no nc c no nc c ground ground +12V ground ground outputs analog input/ 5th contact input open collector outputs contact inputs For liquid handlers, the analog input is normally intended for connection to a detector but may be used for other specific user applications. Microswitch Settings The eight micro-switches on the rear panel of the liquid handler serve two purposes. They are used to address the liquid handler and to activate certain functions such as the baud rate, local clock, etc... Microswitch Function Up Down no 7 Clock Local clock is selected only if Local clock is always enabled RS-232 socket is being used 6 Baud speed Baud speed = bps Baud speed = 9600 bps 5 Analog Input Input is normal Input acts as 5th contact 4 Unit ID number (in binary), 16 0 most significant bit 3 Unit ID Unit ID Unit ID Unit ID number (in binary), 1 0 least significant bit 2-6

19 Description 2 Each switch is off when up and on when down. The unit is factory set at 10. The diagram below represents microswitches which have been set at a GSIOC address of up down Electrical Assembly Default setting 2-7

20 Description 2 Rinsing Stations Rinsing well for level sensing Rinsing station plug Rinsing well Drain Rinsing Stations Liquid handlers are equipped with rinsing stations situated at the extremities of the support bar*. For the 221 model there is only one rinsing station located on the left hand-side of the support bar. As the 222 model is larger, 2 rinsing stations are provided, one at either end of the support bar. The reason for this is to speed up the rinsing process. When the needle is left of center of the support bar during operation the needle is rinsed in the rinsing station to the left of the bar and in the rinsing station to the right of the bar if right of center. Although this configuration is standard for a number of Gilson applications, the user can, if desired, program the needle to be rinsed in one rinsing station only. Rinsing stations serve to clean the needle both externally and internally. * There are three positions for mounting the rinsing stations on the support bar: A, left side, B, the right of the center, and C on the right end of the support bar. Positions A and C are compatible with older versions of 222. However, in this configuration, the right station is close to the right valve. Position B offers better access to the right injection valve and is recommended as the standard installation for the right rinsing station on new samplers. The rinsing process is performed as follows: 1) A predetermined volume of rinsing solvent is flushed through the needle, removing bubbles from the FEP tubing and flushing any insoluble matter from the needle. This solvent is dispensed in the rinsing station drain. 2) The needle is then plunged into the rinsing well containing the solvent, which serves to rinse the needle externally. Another volume of rinsing solvent is then dispensed in the rinsing well. Excess solvent in the rinsing well overflows into the drain leading to a waste receptacle. When the liquid level detector is activated the smaller rinsing well is used. The user has the possibility of removing the larger rinsing well and replacing it with a special plug if the liquid level detector is to be used for long periods. This prevents the larger rinsing well from filling up with sediment. Whenever the standard support bar is replaced, the user must remember to remove the rinsing station from the standard support bar and install it on the replacement bar. 2-8

21 Installing a Liquid Handler 3 The liquid handler is delivered, factory-fitted with support bar, rinsing station (1 for the 221 model and 2 for the 222 model) and tray holding bar (refer to the figures on the next page). For safety purposes, the vertical arm is not installed but is supplied in the parts compartment of the packing. The vertical arm must be installed by the user (refer to the procedure described in chapter Installing the Vertical Arm ). 3-1

22 Installing a Liquid Handler XL Rinsing station 221 front panel Support bar Tray Tray holding bar 222 XL 222 front panel Support bar Rinsing station Tray Tray rail 3-2

23 Installing a Liquid Handler 3 Power Connection/Fuses Voltage Selection The sector block situated on the rear panel of the unit contains the ON/OFF switch, power socket and fuse holder/voltage selector. Both the 221 and 222 models are pre-set to operate on 240V. In order to change the voltage configuration, check that the ON/OFF switch is in the OFF position and the power cord is not connected to the machine. Then, using a small screw-driver or similar, pry the fuse holder open and remove it from the power receptacle. Power Connection/Fuses The voltages, either 110/120V or 220/240V indicated at the rear of the fuse holder can be changed by simply turning over the fuse holder, such that the arrow corresponding to the required voltage, points to the alignment mark on the power receptacle body. It is important to check that the voltage selected corresponds to that of the power supply used. Fuses The instrument requires two fuses to be installed. The type of fuses required are 0.5 Amp type T slow blow. For safety reasons, liquid handlers are delivered without fuses installed. Fuses must be installed by the user upon delivery. Connecting the power cord Make sure the ON/OFF switch is in the OFF (down) position. Connect the power cord to the power socket at the rear of the unit and then plug in to the power supply source. When the power cord has been connected and the ON/OFF switch is put to the ON position, a green LED on the front carriage comes on. 3-3

24 Installing a Liquid Handler 3 Installing the Vertical Arm a) b) This diagram shows the underside of the horizontal arm and slider. c) d) e) Installing the Vertical Arm Before installing or changing the vertical arm, check that the ON/OFF switch is in the OFF (down) position and that the power cord has been disconnected from the unit s power socket. Check also that the horizontal arm locking screw has been removed. a) Remove the black nylon screw from the tip of the horizontal arm by turning it counterclockwise. b) Carefully extract the hexagonal control rod by pulling it outwards. c) Move the slider to an intermediate position on the horizontal arm. d1)insert the vertical arm into the horizontal slider. When viewed from above, the needle holder must be on the right-hand side. d2)firmly secure the vertical arm by inserting the two screws supplied, into the underside of the slider and vertical arm. d3)connect the power cord and switch the ON/OFF switch to its ON position. Wait while the motors are reset to zero. d4)when the arm has moved to its zero position, move the needle holder upwards gently until it stops. e1) Insert the hexagonal rod into the horizontal arm so that it passes through the vertical arm s gearing and into the motor drive socket. e2) Move the needle holder downwards by about 1 mm from the upper limit, at the same time gently push the rod until it clicks into position. e3) Replace and tighten the black nylon screw. Switch the ON/OFF switch to its OFF position. f) If the size of the arm installed is not the same as the one offered by Gilson in the standard package, the Z-arm must be calibrated. Use the Z-arm calibration software for XL samplers, or call the local Gilson distributor for calibration. The assembly process must be performed in the order specified above so that the mechanical and electrical components of the unit are coordinated. The unit cannot operate correctly if the needle holder is significantly more than 1 mm away from the vertical arm s upper limit. 3-4

25 Installing a Liquid Handler 3 Needle/Liquid Level Detector The liquid level detector cable is an accessory. When it is installed, the instrument no longer conforms to EMC requirements. A special feature of 221 and 222 Liquid Handlers is the liquid level detector. It is used to detect when the needle has reached the liquid surface which permits the instrument to calculate the change in the liquid level so that the tip of the needle always remains a predetermined distance below the surface. It can operate only when the liquid handler is used in conjunction with a 402 Syringe Pump or a 401 Dilutor. It does not function when the Minipuls 3 peristaltic pump is used. The purpose of the liquid level detector is to reduce the surface of the needle coming into contact with the liquid thus reducing contamination Needle/Liquid Level Detector Principle of the Liquid Level Detector The needle descends into the tube until it reaches the surface of the liquid. When this happens, a signal is sent to the processor. From the moment the processor receives this signal, it starts to calculate the vertical speed required for the needle to follow the liquid level as it changes. The vertical speed of the needle is calculated as a function of the flow rate and the cross sectional area of the tube entered. Once the processor knows the rate at which the level in the vial changes, it can adjust the speed of the needle to remain a constant distance below the level of the liquid. Sensitivity Limits The liquid level detector can only detect certain liquids which have a dielectric constant greater than 18 (for a sensitivity value of 3). This means that almost all water-soluble solvents (i.e. aqueous solutions, common alcohols, acetone and acetonitrile) are easily detected by the liquid level detector. Among the solvents which cannot be detected are hydrocarbons such as hexane, cyclohexane and benzene and chlorinated solvents such as chloroform and dichloromethane. 3-5

26 Installing a Liquid Handler 3 Needle/Liquid Level Detector During normal operation, the recommended immersion depth for the needle is 2 mm. It is important to note that: The liquid level detector is not compatible with septum piercing. The reason for this being that with septum piercing, the needle sleeve must always enter the tube to compensate for the decrease of air pressure within the tube when the liquid is aspirated. The liquid level detector does not function for code 8 racks. The liquid level detector only functions with code 9 racks if the 402 Syringe Pump or 401 Dilutor is installed with either a 0.5 ml or 1 ml syringe. The maximum recommended aspiration flow rates in order to avoid immersion errors, are: - 7 ml/min for small vials (in general, vials of volume 4 ml or less), - 10 ml/min for open tubes, - 20 ml/min for 20 ml vials. When the liquid level detector is activated, a precise value must be entered for the inner diameter of the vial. The vial diameter is measured in tenths of millimeters. The Gilson program offers a default value for each vial diameter at the programming stage of the operation. As the rate at which the needle descends or rises in the vial depends on the inner diameter of the vial, it is important to enter a value of within one tenth of a millimeter. Below are some examples of possible errors generated by entering the wrong internal tube diameter: For a code 21 rack, the default tube diameter is 11.3 mm. If there is an error of +/- 0.1 mm entered for the tube diameter, then the resulting immersion error will be +/- 1.5 mm. If there is an error of +/- 0.5 mm entered for the tube diameter then the resulting immersion error is +/- 7 mm. For a code 9 rack, the default tube diameter is 6.1 mm. If there is an error of +/- 0.5 mm entered for the tube diameter, then the resulting immersion error will be +/- 4 mm. The liquid level detector sensitivity can be adjusted by the user at the programming stage of the operation. For further information on sensitivity levels, etc. refer to the software manual. 3-6

27 Installing a Liquid Handler 3 Installation To install the needle/liquid level detector: 1) Slide the needle into the tubing fitting. 2) Select the FEP (polytetrafuoroethylene) transfer tubing for the unit to dilutor connection which corresponds to the volumes to be transferred. 3) Connect the selected tubing to the fitting. 4) Slide the needle assembly into the needle holder on the vertical arm so that it passes through the needle guide then connect the other end of the tubing to the outlet of the 402 Syringe Pump, 401 Dilutor or the Minipuls 3 Peristaltic Pump. Ring contact Needle FEP tubing Tubing fitting Needle holder Needle/Liquid Level Detector 5) Slide the ring contact of the liquid level detector wire into the slot provided on the needle holder. Check that the contact enters the slot fully so that the tightening screw passes through the ring. This is important as failure to do so could lead to liquid level detection errors. Liquid level detector wire 6) Connect the liquid level detector wire to the coaxial socket situated just above the power-on LED at the front of the carriage casting. 7) If larger diameter needles or probes such as teflon coated probes are used, you may need to mount specific needle holders and needle guides instead of the standard ones. Needle guide 3-7

28 Installing a Liquid Handler 3 Gilson Sampler Controller Keypad Gilson Sampler Controller Keypad The Liquid Handlers are available with either 735 Sampler Controller Software or with the Keypad Controller and the 720 software. For installation of the 735 Sampler Controller Software, refer to the relevant User s Guide. The Sampler Controller Keypad supplied in the parts compartment of the packing assembly controls the liquid handler and connected pumps. The keypad is connected to the instrument via an RS232 coiled cable. Characters are displayed in white on a blue background. The keypad consists of: - an eight-line, 40 character visual display unit, - five menu soft-touch keys just below the display unit, - a numerical keypad with an ENTER soft-touch key, - four cursor direction soft-touch keys, - a group of three function soft-touch keys, HELP, ESC., CLEAR. - a 3.5" diskette drive with protective cover. Type of diskettes used are 3.5" high density diskettes. For information on using the keypad refer to the software manual. 3-8

29 Installing a Liquid Handler 3 Pumps Liquid handlers can operate in conjunction with a 402 Syringe Pump, 401 Dilutor or a Gilson Minipuls 3 Peristaltic Pump. Pumps 402 Syringe Pump The 402 Syringe Pump is a slave pump and is connected to either the 221 or 222 Liquid Handler via a GSIOC (Gilson Serial Input/Output Channel) connector located on the rear panel of the 402 Syringe Pump. Refer to the 402 User's Guide. 401 Dilutor The 401 Dilutor is a slave pump and is connected to either the 221 or 222 Liquid Handler via a GSIOC (Gilson Serial Input/Output Channel) connector located on the rear panel of the 401 Dilutor. Refer to the 401 User s Guide. Minipuls 3 Peristaltic Pump The Minipuls 3 Peristaltic Pump is connected to 221 and 222 Liquid Handlers via a GSIOC connector. It is important to note that the liquid level detector does not function with a Minipuls 3 Peristaltic Pump, and that you cannot aspirate with the Minipuls 3. Refer to the Minipuls 3 Peristaltic Pump User's Guide. 3-9

30 Installing a Liquid Handler

31 Installing the Additional Accessories 4 The reference and descriptions of the additional accessories are available in Appendix A, Accessories. 4-1

32 Installing the Additional Accessories 4 Heightening Kit Heightening kit This kit is used to heighten the unit so that longer tubes may be used. When this kit is used, a 183 mm stroke arm must be installed. Using this kit means that racks are used in the lower position. When the heightening kit is installed, racks of codes 22, 33 and 34 should be entered as 92, 93 and 94 respectively. It is available for Model 222 Liquid Handlers only. It contains 4 heightening legs and two tray rail support bars. The legs are fitted to the base of the liquid handler. To install these legs simply place the liquid handler on its side and locate the four holes situated at each corner of the base of the unit. Once located, simply screw-in the four heightening legs and redress the unit. The two tray rail support bars are fitted to the front panel of the liquid handler using the four screws supplied. The tray rail is fitted to the tray rail support bars via the hole situated near the base of each tray rail support bar. Support bar Tray rail Heightening legs Tray rail support bar 4-2

33 Installing the Additional Accessories 4 Metallic Front Tray Assembly The metallic front tray assembly is a heightening and support kit which is available for 222 Liquid Handlers only. It is used for certain applications (i.e. pathological tissue staining, glue dropping, etc.) where it is necessary to have a flat working surface. The metallic front tray assembly consists of: - a tray platform, - two platform support arms, - two adjustable supports, - four heightening legs (65 mm). Metallic Front Tray Assembly Installation The user may install the tray platform in either the upper or lower position, depending on the application, and on the length of the vertical arm installed on the liquid handler (lower position for the 183 mm arm). Four screws, with black plastic knurled heads, are provided for the purpose of attaching the tray platform to the platform support arms. The tray platform is fixed either above the platform support arms for the 'upper' position, or below the platform support arms with the tray rail uppermost and nearest to the 222 for the 'lower' position. Support bar Platform support arm Tray rail Tray platform Adjustable support Heightening legs 4-3

34 Installing the Additional Accessories 4 Metallic Front Tray Assembly To install the legs simply place the liquid handler on its side and locate the four holes situated at each corner of the base of the unit. Once located, simply screw-in the four heightening legs and redress the unit. The two metallic platform support arms are fitted to the front panel of the unit using the four screws supplied. When a 183 mm vertical arm is used the tray platform must be in the lower position. This is to avoid damage to the needle during operation. When a 123 mm vertical arm is used, the tray platform must be in the upper position. Installing the Platform in the Upper Position To install the tray platform simply: 1) Remove the two plastic headed screws from the top of the support arm. 2) Place the platform on the arm, with the tray rail uppermost and nearest the arm angle. Replace the two screws by passing them through the two corresponding holes in the platform. Secure them manually. 3) Repeat the procedure for the other arm. 4) Install the platform and arm assembly onto the front panel of the machine using the two screws provided on each arm. Installing the platform in the lower position For some applications (notably, when using the 183 mm stroke arm) it is necessary to install the platform in its lower position, beneath the support arms as follows: 1) Remove the two plastic headed screws and the adjustable support from the support arm. 2) Position the platform beneath the support arm with the tray rail uppermost and nearest the angle. (i.e. the tray rail towards the machine when the assembly is installed). 3) Locate the two smaller holes in the base of the support arm and position the arm such that these holes correspond to the two smaller holes in the platform. 4-4

35 Installing the Additional Accessories 4 4) Secure the platform to the arm from beneath using the two plastic headed screws. 5) Locate the third hole in the base of the platform and insert the adjustable support. 6) Repeat the procedure for the other arm. 7) Install the platform and arm assembly onto the front panel of the machine using the two screws provided on the arm. Levelling the tray platform The tray platform must be horizontal for correct needle operation. Two adjustable supports are provided on the underside of each support arm for this purpose. Metallic Front Tray Assembly Adjustment is performed as follows: 1) Manually loosen the support s locking collars located at the top of each support, by turning them clockwise. 2) Turn the foot of each support to level the tray platform. Check, using a spirit level, that the workbench, liquid handler and platform are level. However, this adjustment is not so critical as to require an absolutely level reference surface. 3) Manually tighten the support s locking collars, by turning them counterclockwise (when viewed from above). Installing the rack-tray Place the rack-tray on top of the tray platform with the groove on the underside of the rack-tray firmly slotted over the tray rail. 4-5

36 Installing the Additional Accessories 4 Vertical Arms Vertical Arms The installation procedure for the long and short arms and needle kits is as described in Chapter 3. When the user has changed the size of the vertical stroke arm, it is necessary to call the local Gilson distributor for recalibration of the machine as the machine will take the arm size as being 123 mm by default. 4-6

37 Installing the Additional Accessories 4 Needles Various needle types are available for use with both 221 and 222 Models. They are: Needles Constricting needles When using TransferPorts, beveled edge constricting needles must be used. These needles are specifically designed for dealing with small volumes. They have a specially designed tip which reduces the risk of cross contamination between samples and increases measuring accuracy when dealing with small volumes. Refer to Chapter 3 for installation. Teflon needles Teflon needles are chemically inert needles. They are used for applications where metal traces such as stainless steel and titanium, from which needles are normally made, should be avoided. Refer to Chapter 3 for installation. Double needles Double needles consist of both an inner and an outer tube which act as two separate needles. They can either be used to mix two different samples in the same vial or for sample agitation or evaporation. Sample agitation and evaporation is performed by sending a gas into the vial through either the inner or outer tube and the sample through the remaining tube. The gas creates bubbles which agitate the sample. Refer to the instructions leaflet supplied with the double needle kit for installation. 4-7

38 Installing the Additional Accessories 4 TransferPorts TransferPort holding bar Plastic cap Connector Connector tubing Yellow cap TransferPorts In some configurations, TransferPorts may be used to aspirate large volumes of either a sample or a solvent. The 221 has 8 possible positions and the 222 has 15 possible TransferPort positions. As TransferPorts are an additional accessory, it is necessary to replace the standard support bar with the specific TransferPort holding bar (ref for 221 and for 222) which consists of a series of numbered holes. To change the standard support bar simply unscrew the knurled screws securing it and replace it with the transfer port holding bar. A bevelled edge constricting needle must be installed by the user if TransferPorts are to be used. Installation 1) Users are advised to place the bottles containing the samples which are to be aspirated through the TransferPorts, at the same level or higher, than the rack tray. 2) When working with Transfer- Ports, the user must always use a bevelled edge constricting needle. 3) TransferPorts must be primed using the program before being used. TransferPort is a trademark of Gilson. TransferPorts are installed as follows: 1) Place the TransferPort in the appropriate mounting hole on the transfer port holding bar and screw the TransferPort and tubing connector together. 2) Fit a yellow cap to the top of the TransferPort. 3) Connect the other end of the male tubing to a filter, and place it in a solvent or sample bottle. 4) Fix the plastic clip onto the TransferPort. 4-8

39 Installing the Additional Accessories 4 Injection Ports When a sample is prepared, it can be injected into a peripheral instrument via an injection port which can be installed on the support bar. Installation Injection Ports The injection port is installed in the hole supplied to the right hand side of the standard support bar as follows: 1) Separate the upper part of the injection port, (with hexagonal head) from the main body by unscrewing it. 2) Insert the upper part of the injection port into the specific hole to the right of the support bar and screw in the main body from beneath. When the seal ( ) is worn, simply extract it using a needle or similar and replace it. 4-9

40 Installing the Additional Accessories 4 Thermostated Racks Thermostated Racks Thermostated racks consist of an aluminium rack and a thermostating cuvette. There are two types of thermostating cuvette: one regulated by a circulating fluid, and the other regulated by Peltier effect cells, electronically controlled by the Gilson 832 Temperature Regulator. They are used to regulate the sample temperature, e.g. cooling for the preservation of biological samples and moderate heating for chemical derivatization. Possible temperatures range from 4 to 60 C for the fluid regulated cuvette and 4 to 40 C for the peltier effect thermostating cuvette. The fluid regulated cuvette is available under reference The Peltier effect thermostating cuvette is available under reference and must be used in conjunction with the Gilson 832 Temperature Regulator, reference Installation When the aluminum thermostating racks are used on the 222, some of them can be placed different positions. As a result, the identification code used for the rack changes so that the different rack and vial coordinates can be recognized by the software. The rack code is the number by which the rack is known to the software. This is extremely important to remember at the programming stage of the application. Refer to Appendix A for the correct code number, reference number and corresponding position of all racks. The unperforated rack must be declared as a custom rack. Rack code Actual code Position Equipment required for software on rack 30 (30) Normal Metallic Holder ( ) and thermostating cuvette. 31 (31) Normal Metallic Holder ( ) and thermostating cuvette. 32 (32) Normal Metallic Holder ( ) and thermostating cuvette. 33 (33) Normal Metallic Holder ( ) and thermostating cuvette. 34 (34) Normal Metallic Holder ( ) and thermostating cuvette. 93 (33) Low* Heightening kit for sampler and thermostating cuvette. 94 (34) Low* Heightening kit for sampler and thermostating cuvette. Custom rack no code Normal Metallic Holder ( ) and thermostating cuvette. This rack ( ) is unperforated (a free rack) and must be drilled according to your requirements, then declared in the PC software tray configuration. 4-10

41 Installing the Additional Accessories 4 Installing a Thermostated Rack on the 221 XL 1) Remove the standard support bar and install the specific thermostated rack holding bar (reference M). 2) Install the rack in the thermostating cuvette. 3) Position the cuvette containing the rack, on the rack holding bar. 4) Connect the hydraulic connections/connect the control cable to the 832 if the thermostating cuvette is the Peltier effect type. Installing a Thermostated Rack on the 222 XL 1) For the racks installed on the tray, install the heightening kit on the sampler (reference ). Follow the instructions supplied with the kit. 2) For the racks in the normal position, replace the standard support bar with the rack holding bar supplied in the rack holder kit (reference ). Fix the rack holder to the tray rail following the instruction supplied with the kit. 3) Loosen the two nylon screws on the front panel of the rack holder. 4) Tilt the front panel forwards. 5) Place the rack inside the thermostating cuvette. * Racks in the normal position are placed in the thermostating cuvette and mounted on the metallic rack holding bar. Racks in the low position are placed in the thermostating cuvette and mounted on the tray. The sampler is in the raised position using the heightening kit, reference Thermostated Racks Rack holding bar supplied in the rack holder kit Thermostated rack holder Tray rail (installed on instrument) When installing a thermostated rack on the 222 model, there are two positions: 'normal' for a rack installed on the rack holding bar (kit reference ) 'low' for a rack installed on the tray the sampler must be fitted with a heightening kit (kit reference ). 4-11

42 Installing the Additional Accessories 4 Thermostated Racks 6) Install the cuvette in one of the five possible positions. Each position is marked by two opposite pins which serve to locate and secure the cuvette. One pin is situated on the holding bar, the other at the rear of the front panel of the rack holder. Position the notches on the polypropylene cuvette over the locating pins. 7) Tighten the two black nylon screws on the front panel of the rack holder. 8) Connect the hydraulic connections/connect the control cable to the 832 if the thermostating cuvette is the Peltier effect type. Perforated Covers for Thermostated Racks When thermostated racks are used to cool samples below the ambient temperature, condensation forms on the surface of the rack and falls into the rack wells. This water accumulates in the wells and forces the vials in the rack to rise. If this happens, needle movement may be obstructed and the needle damaged. For this reason stainless steel perforated covers have to be installed. They are clipped onto the rack handles as shown. These covers are available for rack codes 30A, 31A, 33A and 34A. Below a list of the perforated covers available. Reference Description A rack cover, stainless steel, 60 holes A rack cover, stainless steel, 108 holes A rack cover, stainless steel, 14 holes A rack cover, stainless steel, 36 holes 4-12

43 Installing the Additional Accessories 4 Universal Rack Universal racks (code 22U) are designed to receive 44 vessels of varying sizes. The sizes of these vessels range from 10 mm to 18 mm external diameter and from 100 to 180 mm in length. Each of the reception cavities contains 4 positioning and retaining clips. Universal Rack 4-13

44 Installing the Additional Accessories 4 Free Racks Free Racks Free racks are blank racks with no vial positions drilled. These racks can only be used with softwares that offer free rack programming (custom rack option), essentially PC based softwares (735 and 719). Reference is a free rack in polypropylene. Reference is a free thermostating rack in aluminum. Drill the vial positions according to your method requirements, then declare the coordinates in the software configuration. 4-14

45 Installing the Additional Accessories 4 Solvent Racks Solvent racks code 60 (reference ) and 61 (reference ) hold up to four solvent bottles. Gilson supplies two types of solvent bottles, 250 ml polypropylene bottles and 180 ml glass bottles. Solvent Racks Select bottles which are comecure the bottles in position and ensure that the needle penetrates each bottle cap correctly. When using rack code 61, a 183 mm vertical stroke arm, corresponding needle and the heightening kit must be installed. Code 60 Code

46 Installing the Additional Accessories 4 Needle Wiper Needle wiper Needle wipers are used to reduce contamination risks when using rack codes 20, 21, 28 or 29. They wipe excess liquid from the exterior of the needle as it is being withdrawn from the sample. Needle wipers consist of three perforated plastic plates between which are held two sheets of absorbent paper. Refer to the opposite figures for the needle wiper assembly. 4-16

47 Installing the Additional Accessories 4 Low Pressure Valve The low pressure valve is an electronically operated (12 VDC) 3-port, 2-position valve and is used for the purpose of evaporating a solvent with an inert gas or mixing liquids contained in a vial by permitting the passage of air into the liquids (the MIX by air method - refer to the software User s Guide. Low Pressure Valve The valve has three connections: two inlets, a normally closed (NC) inlet, and a normally open (NO) inlet; and one common (COM) outlet. This is the default position of an unpowered valve. The valve receives its power from the connector on the carriage casting. Installation 1) Remove the screw situated on the upper left hand corner of the front panel of the vertical arm. 2) Slide the valve attachment piece onto the front panel of the vertical arm until the hole at the top left hand corner of the attachment piece corresponds to the hole left in the arm by the removal of the screw. 3) Fix the valve to the vertical arm using the attachment screw supplied. 4) Connect the 402 Syringe Pump, 401 Dilutor or Minipuls 3 Peristaltic Pump to the NO (normally open) inlet port of the valve. 5) Connect the FEP tubing, (already connected to the needle assembly), to the valve outlet port. 6) Connect the air supply (or inert gas or alternative fluid) to the valve via the NC (normally closed) inlet port. 7) Once hydraulic and pneumatic connections have been made, the valve can be connected to the 12 VDC output situated just below the power-on LED at the front of the carriage casting. The maximum allowable working pressure of the valve is MPa. 4-17

48 Installing the Additional Accessories

49 Maintenance 5 The ON/OFF switch must always be switched to OFF and the unit disconnected from the mains whenever a maintenance operation is to be performed. Only maintenance operations described here should be carried out by the user. Other maintenance operations not described here should be referred to qualified service personnel. 5-1

50 Maintenance 5 Cleaning Cleaning Cleaning, dismantling, maintenance, adjustment and repair should only be performed by personnel trained in such work, and who are aware of the possible dangers involved. These instruments must not be sterilized, using an autoclave, or any other mechanical device. When you need to clean an instrument, use one of the three following methods: 1) A clean dry cloth. 2) A cloth dampened with water. 3) A cloth dampened with soapy water. If a cloth dampened with soapy water is used to clean the instrument, only domestic soap may be used. No other form of detergent or chemical may be used. 5-2

51 Maintenance 5 Lubrication Model 221 and 222 Liquid Handlers should never be lubricated. The user is strongly advised against oiling or greasing any moving part such as guiding rods, gears, belts etc. Lubrication 5-3

52 Maintenance 5 Transportation Transportation If the liquid handler is to be transported, the user is advised to remove the vertical arm and replace the horizontal arm locking screw. Failure to do so could result in misalignment or belt breakage. When transporting over shorter distances (i.e. within the laboratory etc...) the liquid handler may be carried by its support bar. Liquid handlers must, on no account, be picked up or carried by either their vertical or horizontal arms as belt breakage or misalignment could occur. 5-4

53 Maintenance 5 Position Alignment Check Liquid handlers leave the factory perfectly aligned and do not normally require checking. As previously mentioned, misalignment may be caused by transporting the unit without having previously secured the horizontal arm or by carrying the unit by its arms. If misalignment should occur, it is possible to check alignment by examining the placement of the needle in the rack position. If there is any doubt about alignment call service. in the recess using a soft cloth or an air line. Position Alignment Check 5-5

54 Maintenance 5 5-6

55 Parts & Accessories Lists Appendix A Parts lists for the 221 and 222 XL Liquid Handlers, consisting of Standard equipment, Accessories, Racks and Spare parts. Appendix A-1

56 Parts & Accessories Lists Appendix A Standard Equipment Standard Equipment Model 221XL Model 222XL Part Number Qty Part Number Qty Description XL Liquid Handler, V XL Liquid Handler with 735 Software, V XL Liquid Handler, V XL Liquid Handler with 735 Software, V Gilson 720 sampler controller software package (only with and ) Needle tubing fitting F F PVDF union connector for 1/4-28 TPI fittings FEP tubing, 500 x 2.5 x 1.5 mm, 800 µl FEP tubing, 1000 x 2.5 x 1.5 mm, 1600 µl Bevelled edge needle, 316L, x 1.5 x 1.1 mm Constricting needle, 316L,160.5 x 1.5 x 1.1 mm Rinsing station drain tubing, 1000 x 6.5 x 4 mm Needle sleeve holder, polyacetal, 2.1 mm hole with screws Rinsing station plug Adhesive retainer for transfer tubing Polypropylene tray mm stroke vertical arm Sampler controller keypad with RS 232 coiled cable GSIOC cable for 22X to 401, 402 or Minipuls connection RS232 cable for connecting sampler to PC Self-adhesive label for GSIOC identification Barrier strip connectors, 6-pin for electrical control socket Liquid level detector wire (400 mm for 221, 480 mm for 222) Power cord V, Hz Power cord V, Hz Fuses 0.5 Amp type T slow blow LT LT and 222 Liquid Handlers User s Guide Appendix A-2

57 Parts & Accessories Lists Appendix A Accessories Arms and needle related items With 221 and 222 Liquid Handlers, there is the possibility of replacing the standard 123 mm stroke vertical arm with either a 56 mm or 183 mm stroke arm. Accessories The 56 mm stroke arm is used for racks in the upper position containing vials with septa. The 183 mm stroke arm is for use with longer tubes. Below is a list of the various accessories which can be used in conjunction with each of the three arms: Model 221XL Model 222XL Part Number Part Number Description For the 56 mm stroke arm mm stroke vertical arm Bevelled edge constricting needle, for use with TransferPorts, 316L, 93.5 x 1.5 x 1.1 mm Constricting needle, 316L, 93.5 x 1.5 x 1.1 mm For the 123 mm stroke arm Side aperture needle for septum piercing and/or liquid mixing, 316L, x 1.5 x 1.1 mm Flat tipped needle for drop transfer, 316L, 165 x 0.7 x 0.4 mm Needle sleeve for , 155 mm, flat tipped without V -cut Teflon needle kit for applications where contact with metal is undesirable (contains: Teflon needle, x 0.9 i.d. ; Special needle holder ; Needle guide) Double needle kit (contains: Flat tipped needle, x 1.5 x 1.1 mm ; Triangular flat tipped needle, x 2.5 x 1.1 mm ; PVDF connector ; Special needle holder ; Needle guide) For the 183 mm stroke arm mm stroke vertical arm Bevelled edge needle, 316L, x 1.5 x 1.1 mm Constricting needle, 316L, x 1.5 x 1.1 mm Bevelled edge constricting needle, for use with TransferPorts, 316L, x 1.5 x 1.1 mm Side aperture needle, for septum piercing and/or liquid mixing 316L, x 1.5 x 1.1 mm Teflon needle for applications where contact with metal is undesirable (contains: Teflon needle, x 0.9 mm i.d. ; Special needle holder ; Needle guide) Heightening kit (contains: 4 heightening legs, 65 mm ; 2 tray rail support bars) Appendix A-3

58 Parts & Accessories Lists Appendix A Accessories Model 221XL Model 222XL Part Number Part Number Description Other Accessories Injection port TransferPort holding bar (221: 8 tubes, 222: 16 holes) TransferPort accessory kit (contains: Polyamide transfer port ; 2800 µl, FEP tubing, 1000 x 3 x 2 mm, fitted with filter 316L, 40 µm ; Sealing caps) Holding bar for racks series 0 to Metallic front tray assembly (contains: Tray platform and arms reference ; 4 heightening legs, 65 mm) Needle wiper for code 20 and 28 racks Needle wiper for code 21 and 29 racks Sheets of wiping paper (pack of 500) port, low pressure valve kit µl FEP tubing (600 x 1.6 x 0.5 mm) µl FEP tubing (100 x 1.6 x 0.8 mm) µl FEP tubing (500 x 2.5 x 1.5 mm) µl FEP tubing (1000 x 2.5 x 1.5 mm) µl FEP tubing (1000 x 3 x 2 mm) ml coiled FEP tubing (2200 x 1.6 x 0.8 mm) ml coiled FEP tubing (1.5 mm i.d.) ml coiled FEP tubing (1.5 mm i.d) ml coiled FEP tubing (1.5 mm i.d.) Cable kit for electrical connections (4 bare-ended wires 1.7 m) pin terminal block for electrical connection M Rack holding bar for one thermostating cuvette Rack holder kit for thermostating cuvettes and series 30 polypropylene racks Guiding bar for rack code A rack cover, stainless steel, 60 holes A rack cover, stainless steel, 108 holes A rack cover, stainless steel, 14 holes A rack cover, stainless steel, 36 holes x 250 ml polypropylene solvent bottles x 180 ml glass solvent bottles glass bottle centering clips polyethylene cap assemblies for solvent bottles FEP injection port seal Polypropylene rinsing well (85 mm) Rinsing station plug Appendix A-4

59 Parts & Accessories Lists Appendix A Gilson Sample Racks Part Number Code Material Max Nb Capacity and type of vials or tubes PP 80 2 ml glass vial PP ml Eppendorf plastic vial, 11 x 40 mm without lids PP 80 9 ml glass tube, 13 x 100 mm POM 96 Standard disposable microtitration plate PP ml glass vial PP ml glass vial PP ml glass tube, 12 x 65 mm K 10 PVDF ml glass tube, 12 x 65 mm Gilson Sample Racks PP ml glass tube, 10 x 100 mm PP 60 9 ml glass tube, 13 x 100 mm , 92 PP or 25 ml glass tube, 18 x 180* or 150 mm U PP 44 Variable size tube: 10 mm to 18* mm diameter, stainless steel, 100* mm to 180 mm height PP 44 8 ml glass or plastic scintillation minivial,17 x 65 mm PP ml glass or plastic scintillation vial, 28 x 60 mm PP or 3.5 ml glass tube, 10 x 65 or 75* mm PP ml glass tube, 12 x 65 mm, 6 ml, 12 x 75* mm Al 60 2 ml glass or plastic scintillation vial, 12 x 32 mm P 30 PP 60 2 ml glass or plastic scintillation vial, 12 x 32 mm Al ml minivial, 7 x 40 mm P 31 PP ml minivial, 7 x 40 mm Al ml glass tube, 12 x 65* mm, 6 ml, 13 x 75 mm or 9 ml, 13 x 100 mm , 93 Al ml glass or plastic scintillation vial, 28 x 60 mm P 33 PP ml glass or plastic scintillation vial, 28 x 60 mm , 94 Al 36 4 ml vial, 15 x 45 mm P 34 PP 36 4 ml vial, 15 x 45 mm SS ml polypropylene or 180 ml glass solvent bottle SS ml polypropylene or 180 ml glass solvent bottle PP variable variable, unperforated free rack for use with PC software Al variable variable, unperforated thermostating free rack for use with PC software PP= Polypropylene; Al= Aluminum; PVDF= Polyvinylydene fluoride; POM= Polyacetal * Only this size of tube can be used with level detection. 1) Racks of codes 20, 21, 28 and 29 are equipped to receive the needle wiper. 2) Racks of codes 92, 93 and 94 for the 222 model only are physically identical to racks of codes 22, 33 and 34 respectively. These codes are used to define the position in which the rack is to be used. Codes 22, 33 and 34 are for the normal position and 92, 93 and 94 are for the lower position when used with a 183 mm stroke arm and heightening kit. Appendix A-5

60 Parts & Accessories Lists Appendix A Gilson Sample Racks Code 0 rack Code 0 rack Code 1 rack Code 7 rack Code 8 rack Code 9 rack Code 10 rack ( = 27) K Code 20 rack Code 21 rack Code 22 rack Code 23 rack Code 24 rack Code 28 rack Code 29 rack Appendix A-6

61 Parts & Accessories Lists Appendix A Code 30 rack (4 x 15 = 60) Code 31 rack (6 x 18 = 108) Code 32 rack (4 x 15 = 60) Gilson Sample Racks Code 33 rack (2 x 7 = 14) Code 34 rack (3 x 12 = 36) Unperforated Aluminium rack Code 30 polypropylene rack (4 x 15 = 60) P Code 31 polypropylene rack (6 x 18 = 108) P Code 33 polypropylene rack (2 x 7 = 14) P Code 34 polypropylene rack (3 x 12 = 36) P Unperforated polypropylene rack Code 60 solvent rack Code 61 solvent rack Appendix A-7