Flash Chromatography Column Guide Medicinal Chemistry Natural Products Proteins & Peptides

Transcription

1 Flash Chromatography Column Guide Medicinal Chemistry Natural Products Proteins & Peptides

2 PuriFlash HC Silica Columns Interchim uses spherical shaped particles instead of conventional flash media (irregular shaped 40-63u). Spherical silica provides 500% higher loading with 70% less solvent and reduces collection volume by 300% PuriFlash HC columns contain a new advanced silica technology based on ultra-high surface area spherical silica. In this example a 25 gram puriflash column purified the same amount of sample loaded on a conventional 40 gram column. For higher loading and increased resolution the 25µ and 15µ columns are recommended. The smaller particle sizes increase resolution and provide up to 5 times the loading compared to conventional flash columns 50µ 25µ Conventional flash columns contain irregular shaped particles Sample Load: 1.0g / 2.5% 40g Conventional Flash 15µ PuriFlash columns contain spherical silica 500% higher loading 70% less solvent 300% less collection volume Sample Load: 1.0g / 4% 25g PuriFlash HC50 Sample Load 1.5g / 6% 25g PuriFlash HC25 50% Less Solvent 30% Less Solvent Partial Purity Sample Load 1.25g / 3% 100% Purity Column 40g Conventional 25g PuriFlash HC50 25g PuriFlash HC25 25g PuriFlash HC15 Collected Sample Load Tubes Volume 1.0 g ml 1.0 g ml 1.5 g ml 3.0 g ml Sample Load 3.0g / 12% 25g PuriFlash HC15 Partial Purity Sample Load 1.25g / 5% Solvent Consumed 480 ml 340 ml 360 ml 460 ml Solvent savings and higher loading based on purifying the equivalent amount of sample on a conventional flash column. 68% Less Solvent Partial Purity / verload Condition Column 40g Conventional PuriFlash HC50 Test Conditions Sample Load 1.25 g 1.25 g Tubes 13 8 Sample: 500 mg/ml dibutyl and diethyl phthalate Flow rate: 20 Solvents: A: Heptane B: EtAc Gradient: 0-7 CV 5% B, 7-13 CV 5-8% B Collected Volume 298 ml 186 ml Solvent Consumed 540 ml 25g 360 ml UV: 254 nm Tube volume: 25 ml % Loading: Sample / silica (w/w)

3 Flash Chromatography Column Guide Medicinal Chemistry Natural Products Proteins & Peptides 1 Column Selection Guidelines General Guidelines Column Selection Chart Column Selection Guide 3 TLC Screening for Flash ptimization 7 Columns Silica HC: High Capacity, HP: High Performance 8, 23 Alumina ALN: Neutral, ALB: Basic 10 Carbon Columns for Decolorization 10 Dry Load Empty or Partial Fill: Celite, Silica, C18 11 Cyano, Diol, HILIC, DEAP, NH2 12, 13 Ion Exchange SCX, SAX, MM1 14 Natural Products P6 14 Reverse Phase C18HC, C18HP, C18HQ 15, 16 Reverse Phase Polymers Atoll X, 302H 16 Reverse Phase High Aqueous RPAQ, C18AQ 17 Biopurification Reverse Phase Bonded Silica C4, C8, C18 (200 & 300Å) Cellufine Ion Exchange, Affinity, HIC, Endotoxin Removal 21, 22 Normal Phase Jumbo Packs 23 Reverse Phase, Linear Scale-up from Analytical to Flash Column Hardware Physical Specifications 25 Column Loading Chart Back Cover

4 2 Column Selection Chart Sample Column Selection Charged Reverse Phase C18HP, C18HC C18HQ, CN Normal Phase HILIC Ion Exchange SCX, MM1, NH2HC, DEAP Acid Sensitive Normal Phase Diol, CN, ALN Reverse Phase Diol Acidic Normal Phase SIHC, SIHP, ALN Reverse Phase C18HP, C18HC C18HQ, CN, P6 Ion Exchange SAX, NH2HC, DEAP Basic Normal Phase SIHC, SIHP, ALN, ALB, CN, NH2HC, DEAP Reverse Phase C18HP, C18HC C18HQ, CN, NH2HC ALB, MM1, DEAP Ion Exchange MM1 Low to Mid Polarity Reverse Phase C18AQ, C18HP, X C18HC, C18HQ, CN, NH2HC, MM1, DEAP Normal Phase Diol, CN High Polarity Normal Phase SIHC, SIHP, ALN, Diol, HILIC Reverse Phase RPAQ, Diol, 302H, NH2HC, DEAP General Guidelines Normal Phase: In normal-phase chromatography, the stationary phase is polar and the mobile phase is less polar. The more polar the solvent, the greater the elution strength. To determine optimum conditions, please refer to TLC screening on page 7. Includes: SIHC, SIHP, ALB, ALN, NH2HC, NH2, DEAP, HILIC, DIL, CN Use: It is possible to re-use puriflash HC & HP columns by flushing with 100% methanol after each run and then completely remove the methanol by purging with air. Storage: Store dry after purging with methanol and air. Do not use hexane as it will soften the polypropylene column hardware. Reverse Phase: In reverse phase chromatography, the stationary phase is non-polar or weakly polar and the mobile phase is more polar. A less polar solvent has higher elution strength. Combinations of water with either methanol or acetonitrile are most often used for gradient elution. Compared to normal phase, reverse phase provides higher efficiency and improved selectivity but lower sample loading capacity. Includes: C18HC, C18HP, C18HQ, C18AQ, RPAQ, 302H, X, CN, NH2HC, NH2, MM1, ALB, P6, DEAP Use: It is possible to re-use puriflash reverse phase columns by flushing with 100% organic after each run. Storage: Remove organic modifiers, buffer salts and flush with 75% Methanol or Acetonitrile in water with end caps in place. Ion Exchange: In ion exchange chromatography, the stationary phase is bonded with ionic functional groups that retain the solute ions of opposite charge. Includes: SCX, SAX, MM1, NH2HC, NH2, DEAP To Retain: Anion Exchange - pka of the target compound < ph < pka of the sorbent Cation Exchange - pka of the sorbent < ph < pka of the target compound To Elute: Anion Exchange - pka of the sorbent < ph < pka of the target compound Cation Exchange - pka of the target compound < ph < pka of the sorbent Storage: Remove buffer salts by flushing with water and store in 100% Acetonitrile with end caps in place.

5 PuriFlash Column Selection Guide 3 Normal Phase, Bare Silica Alumina and Dry-Load Normal Phase: Bare Silica, Alumina, Dry-Load and Decolorization Type Application Capacity Base Material SIHC Non-ionic polar organic compounds. Methanol stable, High Capacity 20% high loading capacity and high resolution. SIHP ALB ALN Non-ionic polar organic compounds. Methanol stable, high separation efficiency at high flow rates. Basic compound purification. Eliminate the use of TEA (triethylamine) to avoid streaking or tailing under normal or reverse phase conditions. Acid sensitive compounds that would degrade if exposed to normal phase silica. 10% 10% 10% High Performance Granular Basic Alumina Granular Neutral Alumina ph Stability Particle Sizes 15, 25 & 50µ 15, 30 & 50µ µ µ Dry-Load Hand tight, no adapters, stable to 15 bar / 215 PSI NA Silica or Celite µ Carbon Decolorization, no exposure to carbon dust 10% Activated Carbon µ Page HC, HP Silica Si H Alumina Basic Al Al Na Na Alumina Nuetral Al Al Normal Phase, Bonded Silica Normal Phase, Bonded Silica Type Application Capacity Base Material NH2HC Polar amine compounds with reduced amine-silanol interaction. Suitable for carbohydrates. High Capacity, High Capacity 7% for higher loading. Eliminate the use of TEA (triethylamine) for basic compounds. NH2 HILIC DIL CN DEAP Polar amine compounds with reduced amine-silanol interaction. Suitable for carbohydrates. Eliminate the use of TEA (triethylamine) for basic compounds. Very polar water soluble compounds; dyes, polyphenols, nucleotides and alkaloids using aqueous solvent systems. Reduce the use of chlorinated solvents using 100% MeH or H2. Reduced rotavap time by using less water. Enhanced selectivity and higher retention for low to medium polarity compounds vs. bare silica. 5% 7% 6% Mid range polarity between bare silica and C18. 6% DiethylAminoPropyl bonded silica. A less polar alternative to NH2. Compatible with MeH and water for use in normal or reverse phase. High Performance High Performance High Performance High Performance ph Stability Particle Sizes µ & 50µ µ & 50µ µ Granular Silica 60µ Page NH2 & NH2 HC CN Diol HILIC Si H Si (CH 2 ) 3 N H H Si H Si (CH 2 ) 3 C N H H Si (CH 2 ) 3 CH 2 CH CH 2 H H H Si (C x ) x +

6 4 PuriFlash Column Selection Guide Reverse Phase, for small molecules Reverse Phase Bonded Silica and Polymers Type Application Capacity Base Material C18HP High Performance Non polar and ionic compounds. Fully end-capped. 3% C18HC C18HQ C18AQ RPAQ Non polar and ionic compounds. Fully end-capped. 4% 3% Non polar and ionic compounds. Fully end-capped. 3% Mid polar compounds. Start gradient with 100% water. 3% Polar compounds. Start gradient with 100% water. 3% High Capacity HPLC Prep Grade, Spherical Silica High Performance High Performance ph Stability Particle Sizes 15, 30 & 50µ & 30µ µ & 30µ & 30µ DEAP DiethylAminoPropyl bonded silica. A less polar alternative to NH2. Compatible with MeH and water for use in normal or reverse phase. Granular Silica 60µ 302H Hydrophilic/hydrophobic polymer for polar and non polar compounds. MW <3 kda. High capacity reverse phase. >25% SDVB Polymer & 70µ Atoll X Atoll X is a universal polymer for mid & non polar compounds, MW <3 kda. High capacity reverse phase. >20% SDVB Polymer µ P6 Amide functionality for aromatic and natural products; flavonoids, chalkones, anthraquinones, aromatic nitro compounds, DNP amino acids, phenols, carbonic acids, acid amides, sulphonic acids and amides of sulphonic acids and amines. 5% Polyamide µ The following phases can also be used for reverse phase: CN, NH2, ALB (page 3) and MM1 (page 5) Page C18HP, C18HC, C18HQ, Si H Si C 18 H 37 RPAQ P H Si C n H n2+1 DEAP H Si (CH 2 ) 3 NH + (CH 2 ) 2 C18AQ Atoll X 302H P6 P H Si C 18 H 37 CH 2 CH CH 2 CH CH 2 CH CH 2 C CH 2 CH 2 CH x P y H N (CH 2 ) 5 C n

7 PuriFlash Column Selection Guide 5 Ion Exchange, Bonded Silica Ion Exchange, Bonded Silica Type Application Capacity Base Material ph Stability Particle Sizes SCX Sulfonic acid functionality which retains weak basic High Performance compounds that have one or more positive charges. 0.3 meq/g Remove or isolate basic compounds µ SAX Quaternary amine functionality which retains strong (pka <1) or weak acids (pka >2). 0.3 meq/g Granular Silica µ MM1 NH2HC NH2 DEAP Unique selectivity of basic, charged and non-polar compounds via ion exchange and hydrophobic chains (SCX/C8). Compounds that possess basic functionality are retained by ion exchange. A washing step with an appropriate ph, elutes ionizable compounds. Retained compounds from hydrophobic bonding are eluted with an organic solvent. Weak anion exchange for strong acids 0.09 meq/g 0.3 meq/g Weak anion exchange for strong acids 0.3 meq/g High Performance High Capacity High Performance µ µ & 50µ Weak anion exchange (secondary amino, pka 10.5) 0.3 meq/g Granular Silica 60µ Page SCX MM1 NH2 & NH2 HC H H Si (CH 2 ) 3 S -H + H H Si (CH 2 ) 3 Si C n H n2+1 S -H + Si H Si (CH 2 ) 3 N H H SAX H H Si (CH N + 2 ) 3 ( ) 3 DEAP H Si (CH 2 ) 3 NH + (CH 2 ) 2

8 H H H R R R R R R 6 PuriFlash Column Selection Guide Reverse Reverse Phase, Phase for for macro-molecules Biopurification Type Application Capacity Base Material PP-C18T Moderately hydrophobic peptides & oligopeptides. 300Å Spherical 2% <40 kda Silica PT-C18T Weak hydrophobic peptides/oligopeptides. <50 kda 2% 200Å Spherical Silica PP-C8 Hydrophobic polypeptides & peptides. <80 kda 2% 300Å Spherical Silica ph Stability Particle Sizes & 30µ & 30µ & 30µ Page PT-C8 Moderately hydrophobic peptides & oligopeptides. <60 kda 2% 200Å Spherical Silica & 30µ 20 PP-C4 Hydrophobic proteins & polypeptides kda 2% PT-C4 Hydrophobic peptides & oligopeptides. <60 kda 2% 300Å Spherical Silica 200Å Spherical Silica & 30µ & 30µ C18T C8 Bio C4 Bio Si Si C 18 H 37 Si Si C 8 H 17 Si Si C 4 H 9 Cellulose Cellulose media Media for biopurification for Biopurification Type Application Capacity Base Material 90A500 (DEAE) 90Q500 (QA) 90C500 (CM) Weak anion exchange, intermediate purification of proteins, peptides and enzymes up to 500 kda. Strong anion exchange, intermediate purification of proteins, peptides and enzymes up to 500 kda. Weak cation exchange, intermediate purification of proteins, peptides and enzymes up to 500 kda. >60 mg/ml BSA >10 mg/ml BSA >70 mg/ml Lysozyme ph Stability Particle Sizes Cellulose Beads µ Cellulose Beads µ Cellulose Beads µ Page S4 (Sulfate) Affinity purification of Virus and heparin binding protein up to 3 kda. >3 mg/ml Lysozyme Cellulose Beads µ 21 90EL Affinity, endotoxin removal up to 2000 kda. 480 µg/ml Cellulose Beads µ 75ES Affinity, endotoxin removal up to 2 kda. 185 µg/ml Cellulose Beads µ 90C4 (butyl) 90PH (phenyl) Hydrophobic interaction for hydrophobic proteins up to 4000 kda Hydrophobic interaction for hydrophobic proteins and enzymes up to 4000 kda 25 mg/ml BSA 30 mg/ml BSA Cellulose Beads µ Cellulose Beads µ

9 TLC Screening for Flash ptimization 7 Prior to running a sample on a flash instrument/column it is important to determine the optimum solubility, selectivity and resolution by TLC. The following solvent systems provide a wide range of selectivities and are a useful screening tool. Valuable information is obtained by running the sample in several mobile phases and will improve the odds of obtaining purity using Flash. It is also useful to create a weak and strong polarity for each of the mobile phases to obtain additional data points. The recommended TLC plate which best correlates to puriflash HC & HP Silica columns is provided by EM Merck, Part number , 2.5 x 7.5cm. Solvent System Compound Characteristics TLC conditions Comments EtAc:Hexanes Lipophilic molecules without ionizable groups Start at 1:1 and adjust until Rf of target is Mixture applied to flash column should be soluble or partially soluble in the mobile phase. Adding DCM will aid solubility but reduce retention. DCM:MeH Lipophilic molecules without ionizable groups If above conditions do not move target at 100% EtAC, add MeH to DCM at 1% up to 15%. If you have an ester functional group in your product, transesterification is possible. This happens when the product ester is exchanged with the solvent alcohol used in the mobile phase - if you have an ethyl ester in your product and you use MeH in the mobile phase you may end up with a small amount of methyl ester in your product. Silica can be acidic and will promote this type of reaction. DCM:MeH + NH3 Molecules with basic amine functionality 90:10 DCM:MeH and adjust MeH until Rf of target is It is common for streaking to occur with basic amines. To reduce streaking/band broadening add an amine modifier (a few drops of NH3 or a solution of NH3 in MeH). Adding an amine modifier can improve compound recovery from the column. DCM:Hexanes: MeH + NH3 Molecules with basic amine functionality 90:10 DCM MeH and adjust Hexanes until Rf of target is If you need to use DCM:MeH but it is still too polar you can add hexanes (or replace the DCM with hexanes). This will improve the Rf without changing the nature of the column and retain the solubilizing power of the mobile phase. Diethyl ether: Hexanes or THF Non-polar molecules Start at 1:1 and adjust until Rf of target is Use methyl tetrahydrofuran instead of THF which does not readily form peroxides.

10 8 PuriFlash Normal Phase HC: High Capacity Silica PF-15SIHCKA PuriFlash Column Kit,15µ Si HC, 4 EACH; 4G, 12G, 25G, 40G $325 KIT PF-15SIHCKB PuriFlash Column Kit,15u Si HC, 1 EACH;80g, 120g, 200g, 300g $625 KIT PF-15SIHCKC PuriFlash Column Kit,15µ Si HC, 2 EACH; 4G, 12G, 25G, 40G $165 KIT PF-25SIHCKA PuriFlash Column Kit, 25µ Si HC, 4 EACH; 4G, 12G, 25G, 40G $195 KIT PF-25SIHCKB PuriFlash Column Kit, 25µ Si HC, 2 EACH; 80G, 120G, 200G, 300G $675 KIT PF-25SIHCKC PuriFlash Column Kit, 25µ Si HC, 1 EACH; 80G, 120G, 200G, 300G $350 KIT PF-50SIHCKA PuriFlash Column Kit, 50µ Si HC, 4 EACH; 4G, 12G, 25G, 40G $135 KIT PF-50SIHCKB PuriFlash Column Kit, 50µ Si HC 2 EACH; 80G, 120G, 200G, 300G $395 KIT PF-15SIHC/4G PuriFlash Column, 15µ Si HC, 4G $220 20/PK PF-15SIHC/12G PuriFlash Column, 15µ Si HC, 12G $530 20/PK PF-15SIHC/25G PuriFlash Column, 15µ Si HC, 25G $485 12/PK PF-15SIHC/40G PuriFlash Column, 15µ Si HC, 40G $715 12/PK PF-15SIHC/80G PuriFlash Column, 15µ Si HC, 80G $505 4/PK PF-15SIHC/120G PuriFlash Column, 15µ Si HC, 120g $725 4/PK PF-15SIHC/200G PuriFlash Column, 15µ Si HC, 200g $620 2/PK PF-15SIHC/300G PuriFlash Column, 15µ Si HC, 300g $775 2/PK PF-15SIHC/800G PuriFlash Column, 15µ Si HC, 800g $1,225 EA PF-15SIHC/1600G PuriFlash Column, 15µ Si HC, 1600g $2,350 EA PF-25SIHC/4G PuriFlash Column, 25µ Si HC, 4g $370 40/PK PF-25SIHC/12G PuriFlash Column, 25µ Si HC, 12g $385 30/PK PF-25SIHC/25G PuriFlash Column, 25µ Si HC, 25g $495 25/PK PF-25SIHC/40G PuriFlash Column, 25µ Si HC, 40g $530 20/PK PF-25SIHC/80G PuriFlash Column, 25µ Si HC, 80g $415 10/PK PF-25SIHC/120G PuriFlash Column, 25µ Si HC, 120g $450 8/PK PF-25SIHC/200G PuriFlash Column, 25µ Si HC, 200g $400 4/PK PF-25SIHC/300G PuriFlash Column, 25µ Si HC, 300g $635 4/PK PF-25SIHC/800G PuriFlash Column, 25µ Si HC, 800g $445 EA PF-25SIHC/1600G PuriFlash Column, 25µ Si HC, 1600g $785 EA PF-50SIHC/4G PuriFlash Column, 50µ Si HC, 4g $275 40/PK PF-50SIHC/12G PuriFlash Column, 50µ Si HC, 12g $305 30/PK PF-50SIHC/25G PuriFlash Column, 50µ Si HC, 25g $320 25/PK PF-50SIHC/40G PuriFlash Column, 50µ Si HC, 40g $320 20/PK PF-50SIHC/80G PuriFlash Column, 50µ Si HC, 80g $335 10/PK PF-50SIHC/120G PuriFlash Column, 50µ Si HC, 120g $370 8/PK PF-50SIHC/200G PuriFlash Column, 50µ Si HC, 200g $310 4/PK PF-50SIHC/300G PuriFlash Column, 50µ Si HC, 300g $425 4/PK PF-50SIHC/800G PuriFlash Column, 50µ Si HC, 800g $260 EA PF-50SIHC/1600G PuriFlash Column, 50µ Si HC, 1600g $530 EA PF-50SIHC-KG PuriFlash 50µ Si HC Bulk $160 KG

11 PuriFlash Normal Phase HP: High Performance Silica 9 PF-15SIHPKA PuriFlash Column Kit, 15µ Si HP, 4 EACH; 4G, 12G, 25G, 40G $475 KIT PF-15SIHPKB PuriFlash Column Kit, 15µ Si HP, 1 EACH; 80G, 120G, 200G, 300G $985 KIT PF-15SIHPKC PuriFlash Column Kit, 15µ Si HP, 2 EACH; 4G, 12G, 25G, 40G $240 KIT PF-30SIHPKA PuriFlash Column Kit, 30µ Si HP, 4 EACH; 4G, 12G, 25G, 40G $195 KIT PF-30SIHPKB PuriFlash Column Kit, 30µ Si HP, 2 EACH; 80G, 120G, 200G, 300G $675 KIT PF-30SIHPKC PuriFlash Column Kit, 30µ Si HP, 1 EACH; 80G, 120G, 200G, 300G $340 KIT PF-50SIHPKA PuriFlash Column Kit, 50µ Si HP, 4 EACH; 4G, 12G, 25G, 40G $145 KIT PF-50SIHPKB PuriFlash Column Kit, 50µ Si HP 2 EACH; 80G, 120G, 200G, 300G $460 KIT PF-15SIHP/4G PuriFlash Column, 15µ Si HP, 4g $220 20/PK PF-15SIHP/12G PuriFlash Column, 15µ Si HP, 12g $530 20/PK PF-15SIHP/25G PuriFlash Column, 15µ Si HP, 25g $485 12/PK PF-15SIHP/40G PuriFlash Column, 15µ Si HP, 40g $715 12/PK PF-15SIHP/80G PuriFlash Column, 15µ Si HP, 80g $505 4/PK PF-15SIHP/120G PuriFlash Column, 15µ Si HP, 120g $725 4/PK PF-15SIHP/200G PuriFlash Column, 15µ Si HP, 200g $620 2/PK PF-15SIHP/300G PuriFlash Column, 15µ Si HP, 300g $775 2/PK PF-15SIHP/800G PuriFlash Column, 15µ Si HP, 800g $1,225 EA PF-15SIHP/1600G PuriFlash Column, 15µ Si HP, 1600g $2,350 EA PF-30SIHP/4G PuriFlash Column, 30µ Si HP, 4g $370 40/PK PF-30SIHP/12G PuriFlash Column, 30µ Si HP, 12g $385 30/PK PF-30SIHP/25G PuriFlash Column, 30µ Si HP, 25g $495 25/PK PF-30SIHP/40G PuriFlash Column, 30µ Si HP, 40g $530 20/PK PF-30SIHP/80G PuriFlash Column, 30µ Si HP, 80g $415 10/PK PF-30SIHP/120G PuriFlash Column, 30µ Si HP, 120g $450 8/PK PF-30SIHP/200G PuriFlash Column, 30µ Si HP, 200g $400 4/PK PF-30SIHP/300G PuriFlash Column, 30µ Si HP, 300g $635 4/PK PF-30SIHP/800G PuriFlash Column, 30µ Si HP, 800g $445 EA PF-30SIHP/1600G PuriFlash Column, 30µ Si HP, 1600g $785 EA PF-50SIHP/4G PuriFlash Column, 50µ Si HP, 4g $265 40/PK PF-50SIHP/12G PuriFlash Column, 50µ Si HP, 12g $295 30/PK PF-50SIHP/25G PuriFlash Column, 50µ Si HP, 25g $310 25/PK PF-50SIHP/40G PuriFlash Column, 50µ Si HP, 40g $310 20/PK PF-50SIHP/80G PuriFlash Column, 50µ Si HP, 80g $325 10/PK PF-50SIHP/120G PuriFlash Column, 50µ Si HP, 120g $360 8/PK PF-50SIHP/200G PuriFlash Column, 50µ Si HP, 200g $275 4/PK PF-50SIHP/300G PuriFlash Column, 50µ Si HP, 300g $415 4/PK PF-50SIHP/800G PuriFlash Column, 50µ Si HP, 800g $250 EA PF-50SIHP/1600G PuriFlash Column, 50µ Si HP, 1600g $390 EA

12 10 PuriFlash Alumina Basic & Neutral PF-ALB/7G PuriFlash Column, 50µ Al Basic, 7g $60 8/PK PF-ALB/25G PuriFlash Column, 50µ Al Basic, 25g $45 4/PK PF-ALB/40G PuriFlash Column, 50µ Al Basic, 40g $60 4/PK PF-ALB/65G PuriFlash Column, 50µ Al Basic, 65g $80 4/PK PF-ALB/135G PuriFlash Column, 50µ Al Basic, 135g $82 2/PK PF-ALB/200G PuriFlash Column, 50µ Al Basic, 200g $115 2/PK PF-ALB/355G PuriFlash Column, 50µ Al Basic, 355g $183 2/PK PF-ALB/550G PuriFlash Column, 50µ Al Basic, 550g $138 EA PF-ALB/1445G PuriFlash Column, 50µ Al Basic, 1445g $342 EA PF-ALB/2900G PuriFlash Column, 50µ Al Basic, 2900g $568 EA PF-ALN/7G PuriFlash Column, 50µ Al Neutral, 7g $59 8/PK PF-ALN/25G PuriFlash Column, 50µ Al Neutral, 25g $56 4/PK PF-ALN/40G PuriFlash Column, 50µ Al Neutral, 40g $77 4/PK PF-ALN/65G PuriFlash Column, 50µ Al Neutral, 65g $106 4/PK PF-ALN/135G PuriFlash Column, 50µ Al Neutral, 135g $109 2/PK PF-ALN/200G PuriFlash Column, 50µ Al Neutral, 200g $156 2/PK PF-ALN/355G PuriFlash Column, 50µ Al Neutral, 355g $253 2/PK PF-ALN/550G PuriFlash Column, 50µ Al Neutral, 550g $194 EA PF-ALN/1445G PuriFlash Column, 50µ Al Neutral, 1445g $483 EA PF-ALN/2900G PuriFlash Column, 50µ Al Neutral, 2900g $857 EA PuriFlash Carbon Columns De-Colorization PF-AC/3G puriflash Carbon 3g, 12 x 64 mm (PF-CARB/4G) $105 16PK PF-AC/10G puriflash Carbon 10g, 21 x 83 mm (PF-CARB/12G) $95 8PK PF-AC/18G puriflash Carbon 18g, 21 x 133 mm (PF-CARB/25G) $125 8PK PF-AC/30G puriflash Carbon 30g, 27 x 133 mm (PF-CARB/40G) $165 8PK PF-AC/60G puriflash Carbon 60g, 31 x 205 mm (PF-CARB/80G) $190 4PK PF-AC/90G puriflash Carbon 90g, 36 x 223 mm (PF-CARB/120G) $255 4PK PF-AC/160G puriflash Carbon 160g, 60 x 161 mm (PF-CARB/200G) $405 4PK PF-AC/250G puriflash Carbon 250g, 60 x 236 mm (PF-CARB/300G) $310 2PK PF-AC/650G puriflash Carbon 650g, 78 x 345 mm $420 EA PF-AC/1300G puriflash Carbon 1300g, 105 x 390 mm $695 EA

13 PuriFlash Dry Load Columns Empty or partially filled with Silica, Celite or C18 11 PF-DLE/4G PuriFlash Dryload Column, Empty 7 ml, 12 x 65 mm (F8880) $80 20/PK PF-DLE/12G PuriFlash Dryload Column, Empty 30 ml, 21 x 80 mm (F8890) $95 20/PK PF-DLE/25G PuriFlash Dryload Column, Empty 75 ml, 27 x 130 mm (FL9521) $125 20/PK PF-DLE/40G PuriFlash Dryload Column, Empty 45 ml, 21 x 130 mm (F8900) $135 20/PK PF-DLSIHC08/4G PuriFlash Dryload Column, HC Silica 3g, 7 ml, 12 x 65 mm $95 20/PK PF-DLSIHC08/12G PuriFlash Dryload Column, HC Silica 11g, 30 ml, 21 x 80 mm $140 20/PK PF-DLSIHC05/25G PuriFlash Dryload Column, HC Silica 20g, 45 ml, 21 x 130mm $340 20/PK PF-DLSIHC05/40G PuriFlash Dryload Column, HC Silica 30g, 75 ml, 27 x 130 mm $195 20/PK PF-50SIHC-CET/200G puriflash Sample loading column, 200G, 50/50 HC/Celite $125 EA PF-DLCET08/4G PuriFlash Dryload Column, Celite 2g, 7 ml, 12 x 65 mm $95 20/PK PF-DLCET08/12G PuriFlash Dryload Column, Celite 5g, 30 ml, 21 x 80 mm $140 20/PK PF-DLCET08/25G PuriFlash Dryload Column, Celite 10g, 45 ml, 21 x 130mm $180 20/PK PF-DLCET08/40G PuriFlash Dryload Column, Celite 15g, 75 ml, 27 x 130 mm $210 20/PK PF-DLSIHC08/25G PURIFLASH-DRY-LAD SILICA-HC-80% 25G FLASH-CLUMN $157 20/PK PF-DLSIHC08/40G PURIFLASH-DRY-LAD SILICA-HC-80% 40G FLASH-CLUMN $189 20/PK PF-DLSIHC05/4G PURIFLASH-DRY-LAD SILICA-HC-50% 4G FLASH-CLUMN $76 20/PK PF-DLSIHC0512G PURIFLASH-DRY-LAD SILICA-HC-50% 12G FLASH-CLUMN $108 20/PK PF-DLSIHC0525G PURIFLASH-DRY-LAD SILICA-HC-50% 25G FLASH-CLUMN $143 20/PK PF-DLIRC808/4G PURIFLASH-DRY-LAD C18-STD-80% 4G FLASH-CLUMN $51 5/PK PF-DLIRC1808/12G PURIFLASH-DRY-LAD C18-STD-80% 12G FLASH-CLUMN $146 5/PK PF-DLIRC1808/25G PURIFLASH-DRY-LAD C18-STD-80% 25G FLASH-CLUMN $232 5/PK PF-DLIRC1808/40G PURIFLASH-DRY-LAD C18-STD-80% 40G FLASH-CLUMN $348 5/PK PF-DLIRC1805/4G PURIFLASH-DRY-LAD C18-STD-50% 4G FLASH-CLUMN $43 5/PK PF-DLIRC180512G PURIFLASH-DRY-LAD C18-STD-50% 12G FLASH-CLUMN $105 5/PK PF-DLIRC1805/40G PURIFLASH-DRY-LAD C18-STD-50% 40G FLASH-CLUMN $240 5/PK PURIFICATIN INSTRUMENTATIN FR ALL Interchim offers the most comprehensive range of instruments from Flash to preparative HPLC HPLC pumps for reliability Detection with UV, ELSD Same software for Flash & HPLC Flash, Evo Series 150, 150 PSI 200, 435 PSI 250, 750 PSI 800, 150 PSI 19" x 17" x 26" Prep HPLC, Spot II 50, 4350 PSI 200, 3300 PSI 500, 1600 PSI 24" x 23" x 26"

14 12 PuriFlash Normal Phase Diol, HILIC & CN Bonded Silica PF-30DIL/6G PuriFlash Column, 30µ Diol, 6g $290 4/PK PF-30DIL/20G PuriFlash Column, 30µ Diol, 20g $285 2/PK PF-30DIL/35G PuriFlash Column, 30µ Diol, 35g $160 EA PF-30DIL/55G PuriFlash Column, 30µ Diol, 55g $200 EA PF-30DIL/120G PuriFlash Column, 30µ Diol, 120g $390 EA PF-30DIL/175G PuriFlash Column, 30µ Diol, 175g $575 EA PF-30DIL/300G PuriFlash Column, 30µ Diol, 300g $1,075 EA PF-30DIL/450G PuriFlash Column, 30µ Diol, 450g $1,655 EA PF-30DIL/1250G PuriFlash Column, 30µ Diol, 1250g $3,410 EA PF-30DIL/2500G PuriFlash Column, 30µ Diol, 2500g $6,825 EA PF-50DIL/6G PuriFlash Column, 50µ Diol, 6g $75 4/PK PF-50DIL/20G PuriFlash Column, 50µ Diol, 20g $109 2/PK PF-50DIL/35G PuriFlash Column, 50µ Diol, 35g $85 EA PF-50DIL/55G PuriFlash Column, 50µ Diol, 55g $130 EA PF-50DIL/120G PuriFlash Column, 50µ Diol, 120g $277 EA PF-50DIL/175G PuriFlash Column, 50µ Diol, 175g $406 EA PF-50DIL/300G PuriFlash Column, 50µ Diol, 300g $701 EA PF-50DIL/450G PuriFlash Column, 50µ Diol, 450g $1,081 EA PF-50DIL/1250G PuriFlash Column, 50µ Diol, 1250g $2,818 EA PF-50DIL/2500G PuriFlash Column, 50µ Diol, 2500g $5,537 EA PF-30HILIC/6G PuriFlash Column, 30µ Hilic, 6g $78 4/PK PF-30HILIC/20G PuriFlash Column, 30µ Hilic, 20g $122 2/PK PF-30HILIC/35G PuriFlash Column, 30µ Hilic, 35g $95 EA PF-30HILIC/55G PuriFlash Column, 30µ Hilic, 55g $149 EA PF-30HILIC/120G PuriFlash Column, 30µ Hilic, 120g $319 EA PF-30HILIC/175G PuriFlash Column, 30µ Hilic, 175g $467 EA PF-30HILIC/300G PuriFlash Column, 30µ Hilic, 300g $813 EA PF-30HILIC/450G PuriFlash Column, 30µ Hilic, 450g $1,250 EA PF-30HILIC/1250G PuriFlash Column, 30µ Hilic, 1250g $3,289 EA PF-30HILIC/2500G PuriFlash Column, 30µ Hilic, 2500g $6,488 EA PF-50CN/6G PuriFlash Column, 50µ CN 6G $89 4PK PF-50CN/20G PuriFlash Column, 50µ CN 20G $138 2PK PF-50CN/35G PuriFlash Column, 50µ CN 35G $111 EA PF-50CN/55G PuriFlash Column, 50µ CN 55G $173 EA PF-50CN/120G PuriFlash Column, 50µ CN 120G $370 EA PF-50CN/175G PuriFlash Column, 50µ CN 175G $543 EA PF-50CN/300G PuriFlash Column, 50µ CN 300G $945 EA PF-50CN/450G PuriFlash Column, 50µ CN, 450g $1,455 EA PF-50CN/1250G PuriFlash Column, 50µ CN, 1250g $3,823 EA PF-50CN/2500G PuriFlash Column, 50µ CN, 2500g $7,563 EA

15 PuriFlash Normal Phase NH2 & DEAP Bonded Silica 13 PF-30NH2/6G PuriFlash Column, 30µ NH2, 6g $280 4/PK PF-30NH2/20G PuriFlash Column, 30µ NH2, 20g $275 2/PK PF-30NH2/35G PuriFlash Column, 30µ NH2, 35g $165 EA PF-30NH2/55G PuriFlash Column, 30µ NH2, 55g $195 EA PF-30NH2/120G PuriFlash Column, 30µ NH2, 120g $409 EA PF-30NH2/175G PuriFlash Column, 30µ NH2, 175g $598 EA PF-30NH2/300G PuriFlash Column, 30µ NH2, 300g $1,037 EA PF-30NH2/450G PuriFlash Column, 30µ NH2, 450g $1,602 EA PF-30NH2/1250G PuriFlash Column, 30µ NH2, 1250g $4,185 EA PF-30NH2/2500G PuriFlash Column, 30µ NH2, 2500g $8,281 EA PF-50NH2/6G PuriFlash Column, 50µ NH2, 6g $95 4/PK PF-50NH2/20G PuriFlash Column, 50µ NH2, 20g $125 2/PK PF-50NH2/35G PuriFlash Column, 50µ NH2, 35g $100 EA PF-50NH2/55G PuriFlash Column, 50µ NH2, 55g $150 EA PF-50NH2/120G PuriFlash Column, 50µ NH2, 120g $320 EA PF-50NH2/175G PuriFlash Column, 50µ NH2, 175g $455 EA PF-50NH2/300G PuriFlash Column, 50µ NH2, 300g $795 EA PF-50NH2/450G PuriFlash Column, 50µ NH2, 450g $1,200 EA PF-50NH2/1250G PuriFlash Column, 50µ NH2, 1250g $3,050 EA PF-50NH2/2500G PuriFlash Column, 50µ NH2, 2500g $6,500 EA PF-50NH2HC/6G PuriFlash Column, 50µ NH2 HC, 6g $43 4/PK PF-50NH2HC/20G PuriFlash Column, 50µ NH2 HC, 20g $57 2/PK PF-50NH2HC/35G PuriFlash Column, 50µ NH2 HC, 35g $43 EA PF-50NH2HC/55G PuriFlash Column, 50µ NH2 HC, 55g $68 EA PF-50NH2HC/120G PuriFlash Column, 50µ NH2 HC, 120g $146 EA PF-50NH2HC/175G PuriFlash Column, 50µ NH2 HC, 175g $211 EA PF-50NH2HC/300G PuriFlash Column, 50µ NH2 HC, 300g $362 EA PF-50NH2HC/450G PuriFlash Column, 50µ NH2 HC, 450g $556 EA PF-50NH2HC/1250G PuriFlash Column, 50µ NH2 HC, 1250g $1,461 EA PF-50NH2HC/2500G PuriFlash Column, 50µ NH2 HC, 2500g $2,822 EA PF-50DEAP/6G PuriFlash Column, 50µ DEAP, 6g $138 6/PK PF-50DEAP/20G PuriFlash Column, 50µ DEAP, 20g $415 6/PK PF-50DEAP/35G PuriFlash Column, 50µ DEAP, 35g $651 6/PK PF-50DEAP/55G PuriFlash Column, 50µ DEAP, 55g $168 EA PF-50DEAP/120G PuriFlash Column, 50µ DEAP, 120g $362 EA PF-50DEAP/175G PuriFlash Column, 50µ DEAP, 175g $527 EA PF-50DEAP/300G PuriFlash Column, 50µ DEAP, 300g $913 EA PF-50DEAP/450G PuriFlash Column, 50µ DEAP, 450g $1,411 EA PF-50DEAP/1250G PuriFlash Column, 50µ DEAP, 1250g $3,684 EA PF-50DEAP/2500G PuriFlash Column, 50µ DEAP, 2500g $7,274 EA

16 14 PuriFlash Ion Exchange SCX & SAX Bonded Silica PF-50SCX/6G PuriFlash Column, 50µ SCX, 6g $165 4/PK PF-50SCX/20G PuriFlash Column, 50µ SCX, 20g $268 2/PK PF-50SCX/35G PuriFlash Column, 50µ SCX, 35g $212 EA PF-50SCX/55G PuriFlash Column, 50µ SCX, 55g $336 EA PF-50SCX/120G PuriFlash Column, 50µ SCX, 120g $719 EA PF-50SCX/175G PuriFlash Column, 50µ SCX, 175g $1,051 EA PF-50SCX/300G PuriFlash Column, 50µ SCX, 300g $1,832 EA PF-50SCX/450G PuriFlash Column, 50µ SCX, 450g $2,830 EA PF-50SCX/1250G PuriFlash Column, 50µ SCX, 1250g $7,416 EA PF-50SCX/2500G PuriFlash Column, 50µ SCX, 2500g ###### Quote EA PF-50SAX/6G PuriFlash Column, 50µ SAX, 6g $275 4/PK PF-50SAX/20G PuriFlash Column, 50µ SAX, 20g $375 2/PK PF-50SAX/35G PuriFlash Column, 50µ SAX, 35g $355 EA PF-50SAX/55G PuriFlash Column, 50µ SAX, 55g $540 EA PF-50SAX/120G PuriFlash Column, 50µ SAX, 120g $865 EA PF-50SAX/175G PuriFlash Column, 50µ SAX, 175g $1,075 EA PF-50SAX/300G PuriFlash Column, 50µ SAX, 300g $1,905 EA PF-50SAX/450G PuriFlash Column, 50µ SAX, 450g $3,060 EA PF-50SAX/1250G PuriFlash Column, 50µ SAX, 1250g $8,260 EA PF-50SAX/2500G PuriFlash Column, 50µ SAX, 2500g $16,450 EA PuriFlash Ion Exchange MM1 Bonded Silica PF-50MM1/6G PuriFlash Column, 50µ MM1 (RP/SCX), 6g $95 4/PK PF-50MM1/20G PuriFlash Column, 50µ MM1 (RP/SCX), 20g $144 2/PK PF-50MM1/35G PuriFlash Column, 50µ MM1 (RP/SCX), 35g $115 EA PF-50MM1/55G PuriFlash Column, 50µ MM1 (RP/SCX), 55g $177 EA PF-50MM1/120G PuriFlash Column, 50µ MM1 (RP/SCX), 120g $377 EA PF-50MM1/175G PuriFlash Column, 50µ MM1 (RP/SCX), 175g $551 EA PF-50MM1/300G PuriFlash Column, 50µ MM1 (RP/SCX), 300g $954 EA PF-50MM1/450G PuriFlash Column, 50µ MM1 (RP/SCX), 450g $1,475 EA PF-50MM1/1250G PuriFlash Column, 50µ MM1 (RP/SCX), 1250g $3,849 EA PF-50MM1/2500G PuriFlash Column, 50µ MM1 (RP/SCX), 2500g $7,610 EA PuriFlash Reverse Phase P6 for Natural Products PF-100P6/2G PuriFlash Column, 100µ P6 Polyamide, 2g $30 4/PK PF-100P6/8G PuriFlash Column, 100µ P6 Polyamide, 8g $40 2/PK PF-100P6/12G PuriFlash Column, 100µ P6 Polyamide, 12g $60 2/PK PF-100P6/20G PuriFlash Column, 100µ P6 Polyamide, 20g $85 2/PK PF-100P6/45G PuriFlash Column, 100µ P6 Polyamide, 45g $88 EA PF-100P6/65G PuriFlash Column, 100µ P6 Polyamide, 65g $124 EA PF-100P6/115G PuriFlash Column, 100µ P6 Polyamide, 115g $209 EA PF-100P6/175G PuriFlash Column, 100µ P6 Polyamide, 175g $321 EA PF-100P6/465G PuriFlash Column, 100µ P6 Polyamide, 465g $819 EA PF-100P6/940G PuriFlash Column, 100µ P6 Polyamide, 940g $1,531 EA

17 PuriFlash Reverse Phase C18HP & C18HQ Bonded Silica 15 PF-15C18HP/6G PuriFlash Column, 15µ C18 HP, 6g $390 4/PK PF-15C18HP/20G PuriFlash Column, 15µ C18 HP, 20g $390 2/PK PF-15C18HP/35G PuriFlash Column, 15µ C18 HP, 35g $225 EA PF-15C18HP/55G PuriFlash Column, 15µ C18 HP, 55g $285 EA PF-15C18HP/120G PuriFlash Column, 15µ C18 HP, 120g $545 EA PF-15C18HP/175G PuriFlash Column, 15µ C18 HP, 175g $736 EA PF-15C18HP/300G PuriFlash Column, 15µ C18 HP, 300g $1,281 EA PF-15C18HP/450G PuriFlash Column, 15µ C18 HP, 450g $1,976 EA PF-15C18HP/1250G PuriFlash Column, 15µ C18 HP, 1250g $5,168 EA PF-15C18HP/2500G PuriFlash Column, 15µ C18 HP, 2500g $10,252 EA PF-30C18HP/6G PuriFlash Column, 30µ C18 HP, 6g $340 4/PK PF-30C18HP/20G PuriFlash Column, 30µ C18 HP, 20g $340 2/PK PF-30C18HP/35G PuriFlash Column, 30µ C18 HP, 35g $195 EA PF-30C18HP/55G PuriFlash Column, 30µ C18 HP, 55g $240 EA PF-30C18HP/120G PuriFlash Column, 30µ C18 HP, 120g $490 EA PF-30C18HP/175G PuriFlash Column, 30µ C18 HP, 175g $540 EA PF-30C18HP/300G PuriFlash Column, 30µ C18 HP, 300g $990 EA PF-30C18HP/450G PuriFlash Column, 30µ C18 HP, 450g $1,490 EA PF-30C18HP/1250G PuriFlash Column, 30µ C18 HP, 1250g $3,445 EA PF-30C18HP/2500G PuriFlash Column, 30µ C18 HP, 2500g $6,995 EA PF-50C18HP/6G PuriFlash Column, 50µ C18 HP, 6g $110 4/PK PF-50C18HP/20G PuriFlash Column, 50µ C18 HP, 20g $118 2/PK PF-50C18HP/35G PuriFlash Column, 50µ C18 HP, 35g $91 EA PF-50C18HP/55G PuriFlash Column, 50µ C18 HP, 55g $141 EA PF-50C18HP/120G PuriFlash Column, 50µ C18 HP, 120g $300 EA PF-50C18HP/175G PuriFlash Column, 50µ C18 HP, 175g $439 EA PF-50C18HP/300G PuriFlash Column, 50µ C18 HP, 300g $760 EA PF-50C18HP/450G PuriFlash Column, 50µ C18 HP, 450g $1,172 EA PF-50C18HP/1250G PuriFlash Column, 50µ C18 HP, 1250g $3,054 EA PF-50C18HP/2500G PuriFlash Column, 50µ C18 HP, 2500g $6,011 EA PF-15C18HQ/6G PuriFlash Column, 15µ C18 HQ, 6g $400 4/PK PF-15C18HQ/20G PuriFlash Column, 15µ C18 HQ, 20g $400 2/PK PF-15C18HQ/35G PuriFlash Column, 15µ C18 HQ, 35g $235 EA PF-15C18HQ/55G PuriFlash Column, 15µ C18 HQ, 55g $295 EA PF-15C18HQ/120G PuriFlash Column, 15µ C18 HQ, 120g $595 EA PF-15C18HQ/175G PuriFlash Column, 15µ C18 HQ, 175g $855 EA PF-15C18HQ/300G PuriFlash Column, 15µ C18 HQ, 300g $1,482 EA PF-15C18HQ/450G PuriFlash Column, 15µ C18 HQ, 450g $2,282 EA PF-15C18HQ/1250G PuriFlash Column, 15µ C18 HQ, 1250g $6,003 EA PF-15C18HQ/2500G PuriFlash Column, 15µ C18 HQ, 2500g $11,925 EA

18 16 PuriFlash Reverse Phase C18HC Bonded Silica PF-15C18HC/6G PuriFlash Column, 15µ C18 HC, 6g $390 4/PK PF-15C18HC/20G PuriFlash Column, 15µ C18 HC, 20g $390 2/PK PF-15C18HC/35G PuriFlash Column, 15µ C18 HC, 35g $225 EA PF-15C18HC/55G PuriFlash Column, 15µ C18 HC, 55g $285 EA PF-15C18HC/120G PuriFlash Column, 15µ C18 HC, 120g $545 EA PF-15C18HC/175G PuriFlash Column, 15µ C18 HC, 175g $736 EA PF-15C18HC/300G PuriFlash Column, 15µ C18 HC, 300g $1,281 EA PF-15C18HC/450G PuriFlash Column, 15µ C18 HC, 450g $1,976 EA PF-15C18HC/1250G PuriFlash Column, 15µ C18 HC, 1250g $5,168 EA PF-15C18HC/2500G PuriFlash Column, 15µ C18 HC, 2500g $10,252 EA PF-30C18HC/6G PuriFlash Column, 30µ C18 HC, 6g $340 4/PK PF-30C18HC/20G PuriFlash Column, 30µ C18 HC, 20g $340 2/PK PF-30C18HC/35G PuriFlash Column, 30µ C18 HC, 35g $195 EA PF-30C18HC/55G PuriFlash Column, 30µ C18 HC, 55g $240 EA PF-30C18HC/120G PuriFlash Column, 30µ C18 HC, 120g $490 EA PF-30C18HC/175G PuriFlash Column, 30µ C18 HC, 175g $540 EA PF-30C18HC/300G PuriFlash Column, 30µ C18 HC, 300g $990 EA PF-30C18HC/450G PuriFlash Column, 30µ C18 HC, 450g $1,490 EA PF-30C18HC/1250G PuriFlash Column, 30µ C18 HC, 1250g $3,445 EA PF-30C18HC/2500G PuriFlash Column, 30µ C18 HC, 2500g $6,995 EA PuriFlash Reverse Phase High Capacity Polymers PF-X/2G PuriFlash Column, Atoll X 40µ 2G $103 4/PK PF-X/7G PuriFlash Column, Atoll X 40µ 7G $159 2/PK PF-X/10G PuriFlash Column, Atoll X 40µ 10G $124 EA PF-X/18G PuriFlash Column, Atoll X 40µ 18G $186 EA PF-X/40G PuriFlash Column, Atoll X 40µ 40G $421 EA PF-X/55G PuriFlash Column, Atoll X 40µ 55G $608 EA PF-X/100G PuriFlash Column, Atoll X 40µ 100G $1,061 EA PF-X/150G PuriFlash Column, Atoll X 40µ 150G $1,628 EA PF-X/400G PuriFlash Column, Atoll X 40µ 400G $4,282 EA PF-X/800G PuriFlash Column, Atoll X 40µ 800G $8,492 EA PF-302H/2G PuriFlash Column, 302H 30µ 2G $143 4/PK PF-302H/7G PuriFlash Column, 302H 30µ 7G $232 2/PK PF-302H/10G PuriFlash Column, 302H 30µ 10G $181 EA PF-302H/18G PuriFlash Column, 302H 30µ 18G $278 EA PF-302H/40G PuriFlash Column, 302H 30µ 40G $621 EA PF-302H/55G PuriFlash Column, 302H 30µ 55G $896 EA PF-302H/100G PuriFlash Column, 302H 30µ 100G $1,577 EA PF-302H/150G PuriFlash Column, 302H 30µ 150G $2,417 EA PF-302H/400G PuriFlash Column, 302H 30µ 400G $6,359 EA PF-302H/800G PuriFlash Column, 302H 30µ 800G ###### Quote EA

19 PuriFlash Reverse Phase High Aqueous C18 Bonded Silica 17 PF-15C18AQ/6G PuriFlash Column, 15µ C18AQ, 6g $390 4/PK PF-15C18AQ/20G PuriFlash Column, 15µ C18AQ, 20g $390 2/PK PF-15C18AQ/35G PuriFlash Column, 15µ C18AQ, 35g $225 EA PF-15C18AQ/55G PuriFlash Column, 15µ C18AQ, 55g $285 EA PF-15C18AQ/120G PuriFlash Column, 15µ C18AQ, 120g $545 EA PF-15C18AQ/175G PuriFlash Column, 15µ C18AQ, 175g $736 EA PuriFlash Column, 15µ C18AQ, 300g $1,281 EA PF-15C18AQ/450G PuriFlash Column, 15µ C18AQ, 450g $1,976 EA PF-15C18AQ/1250G PuriFlash Column, 15µ C18AQ, 1250g $5,168 EA PF-15C18AQ/2500G PuriFlash Column, 15µ C18AQ, 2500g $10,252 EA PF-30C18AQ/6G PuriFlash Column, 30µ C18AQ, 6g $340 4/PK PF-30C18AQ/20G PuriFlash Column, 30µ C18AQ, 20g $340 2/PK PF-30C18AQ/35G PuriFlash Column, 30µ C18AQ, 35g $195 EA PF-30C18AQ/55G PuriFlash Column, 30µ C18AQ, 55g $240 EA PF-30C18AQ/120G PuriFlash Column, 30µ C18AQ, 120g $490 EA PF-30C18AQ/175G PuriFlash Column, 30µ C18AQ, 175g $540 EA PF-30C18AQ/300G PuriFlash Column, 30µ C18AQ, 300g $990 EA PF-30C18AQ/450G PuriFlash Column, 30µ C18AQ, 450g $1,490 EA PF-30C18AQ/1250G PuriFlash Column, 30µ C18AQ, 1250g $3,445 EA PF-30C18AQ/2500G PuriFlash Column, 30µ C18AQ, 2500g $6,995 EA PF-15RPAQ/6G PuriFlash Column, 15µ RPAQ, 6g $390 4/PK PF-15RPAQ/20G PuriFlash Column, 15µ RPAQ, 20g $390 2/PK PF-15RPAQ/35G PuriFlash Column, 15µ RPAQ, 35g $225 EA PF-15RPAQ/55G PuriFlash Column, 15µ RPAQ, 55g $285 EA PF-15RPAQ/120G PuriFlash Column, 15µ RPAQ, 120g $545 EA PF-15RPAQ/175G PuriFlash Column, 15µ RPAQ, 175g $736 EA PF-15RPAQ/300G PuriFlash Column, 15µ RPAQ, 300g $1,281 EA PF-15RPAQ/450G PuriFlash Column, 15µ RPAQ, 450g $1,976 EA PF-15RPAQ/1250G PuriFlash Column, 15µ RPAQ, 1250g $5,168 EA PF-15RPAQ/2500G PuriFlash Column, 15µ RPAQ, 2500g $10,252 EA PF-30RPAQ/6G PuriFlash Column, 30µ RPAQ, 6g $340 4/PK PF-30RPAQ/20G PuriFlash Column, 30µ RPAQ, 20g $340 2/PK PF-30RPAQ/35G PuriFlash Column, 30µ RPAQ, 35g $195 EA PF-30RPAQ/55G PuriFlash Column, 30µ RPAQ, 55g $240 EA PF-30RPAQ/120G PuriFlash Column, 30µ RPAQ, 120g $490 EA PF-30RPAQ/175G PuriFlash Column, 30µ RPAQ, 175g $540 EA PF-30RPAQ/300G PuriFlash Column, 30µ RPAQ, 300g $990 EA PF-30RPAQ/450G PuriFlash Column, 30µ RPAQ, 450g $1,490 EA PF-30RPAQ/1250G PuriFlash Column, 30µ RPAQ, 1250g $3,445 EA PF-30RPAQ/2500G PuriFlash Column, 30µ RPAQ, 2500g $6,995 EA

20 18 PuriFlash Reverse Phase Biopurification 300Å C18 and C8 Bonded Silica PP-15C18T/6G PuriFlash Column, 15µ PP 300Å C18T, 6g $210 2/PK PP-15C18T/20G PuriFlash Column, 15µ PP 300Å C18T, 20g $210 EA PP-15C18T/35G PuriFlash Column, 15µ PP 300Å C18T, 35g $345 EA PP-15C18T/55G PuriFlash Column, 15µ PP 300Å C18T, 55g $545 EA PP-15C18T/120G PuriFlash Column, 15µ PP 300Å C18T, 120g $1,166 EA PP-15C18T/175G PuriFlash Column, 15µ PP 300Å C18T, 175g $1,708 EA PP-15C18T/300G PuriFlash Column, 15µ PP 300Å C18T, 300g $2,989 EA PP-15C18T/450G PuriFlash Column, 15µ PP 300Å C18T, 450g $4,618 EA PP-15C18T/1250G PuriFlash Column, 15µ PP 300Å C18T, 1250g $12,113 EA PP-15C18T/2500G PuriFlash Column, 15µ PP 300Å C18T, 2500g $24,178 EA PP-30C18T/6G PuriFlash Column, 30µ PP 300Å C18T, 6g $170 2/PK PP-30C18T/20G PuriFlash Column, 30µ PP 300Å C18T, 20g $170 EA PP-30C18T/35G PuriFlash Column, 30µ PP 300Å C18T, 35g $205 EA PP-30C18T/55G PuriFlash Column, 30µ PP 300Å C18T, 55g $245 EA PP-30C18T/120G PuriFlash Column, 30µ PP 300Å C18T, 120g $490 EA PP-30C18T/175G PuriFlash Column, 30µ PP 300Å C18T, 175g $598 EA PP-30C18T/300G PuriFlash Column, 30µ PP 300Å C18T, 300g $1,040 EA PP-30C18T/450G PuriFlash Column, 30µ PP 300Å C18T, 450g $1,602 EA PP-30C18T/1250G PuriFlash Column, 30µ PP 300Å C18T, 1250g $4,191 EA PP-30C18T/2500G PuriFlash Column, 30µ PP 300Å C18T, 2500g $8,290 EA PP-15C8/6G PuriFlash Column, 15µ PP 300Å C8, 6g $210 2/PK PP-15C8/20G PuriFlash Column, 15µ PP 300Å C8, 20g $210 EA PP-15C8/35G PuriFlash Column, 15µ PP 300Å C8, 35g $330 EA PP-15C8/55G PuriFlash Column, 15µ PP 300Å C8, 55g $521 EA PP-15C8/120G PuriFlash Column, 15µ PP 300Å C8, 120g $1,119 EA PP-15C8/175G PuriFlash Column, 15µ PP 300Å C8, 175g $1,640 EA PP-15C8/300G PuriFlash Column, 15µ PP 300Å C8, 300g $2,868 EA PP-15C8/450G PuriFlash Column, 15µ PP 300Å C8, 450g $4,429 EA PP-15C8/1250G PuriFlash Column, 15µ PP 300Å C8, 1250g $11,621 EA PP-15C8/2500G PuriFlash Column, 15µ PP 300Å C8, 2500g $23,198 EA PP-30C8/6G PuriFlash Column, 30µ PP 300Å C8, 6g $170 2/PK PP-30C8/20G PuriFlash Column, 30µ PP 300Å C8, 20g $170 EA PP-30C8/35G PuriFlash Column, 30µ PP 300Å C8, 35g $205 EA PP-30C8/55G PuriFlash Column, 30µ PP 300Å C8, 55g $245 EA PP-30C8/120G PuriFlash Column, 30µ PP 300Å C8, 120g $490 EA PP-30C8/175G PuriFlash Column, 30µ PP 300Å C8, 175g $545 EA PP-30C8/300G PuriFlash Column, 30µ PP 300Å C8, 300g $1,005 EA PP-30C8/450G PuriFlash Column, 30µ PP 300Å C8, 450g $1,515 EA PP-30C8/1250G PuriFlash Column, 30µ PP 300Å C8, 1250g $3,687 EA PP-30C8/2500G PuriFlash Column, 30µ PP 300Å C8, 2500g $7,283 EA

21 PuriFlash Reverse Phase Biopurification 300Å C4 and 200Å C18 Bonded Silica 19 PP-15C4/6G PuriFlash Column, 15µ PP 300Å C4, 6g $210 2/PK PP-15C4/20G PuriFlash Column, 15µ PP 300Å C4, 20g $215 EA PP-15C4/35G PuriFlash Column, 15µ PP 300Å C4, 35g $345 EA PP-15C4/55G PuriFlash Column, 15µ PP 300Å C4, 55g $545 EA PP-15C4/120G PuriFlash Column, 15µ PP 300Å C4, 120g $1,166 EA PP-15C4/175G PuriFlash Column, 15µ PP 300Å C4, 175g $1,708 EA PP-15C4/300G PuriFlash Column, 15µ PP 300Å C4, 300g $2,989 EA PP-15C4/450G PuriFlash Column, 15µ PP 300Å C4, 450g $4,618 EA PP-15C4/1250G PuriFlash Column, 15µ PP 300Å C4, 1250g $12,113 EA PP-15C4/2500G PuriFlash Column, 15µ PP 300Å C4, 2500g $24,178 EA PP-30C4/6G PuriFlash Column, 30µ PP 300Å C4, 6g $170 2/PK PP-30C4/20G PuriFlash Column, 30µ PP 300Å C4, 20g $170 EA PP-30C4/35G PuriFlash Column, 30µ PP 300Å C4, 35g $205 EA PP-30C4/55G PuriFlash Column, 30µ PP 300Å C4, 55g $245 EA PP-30C4/120G PuriFlash Column, 30µ PP 300Å C4, 120g $490 EA PP-30C4/175G PuriFlash Column, 30µ PP 300Å C4, 175g $598 EA PP-30C4/300G PuriFlash Column, 30µ PP 300Å C4, 300g $1,040 EA PP-30C4/450G PuriFlash Column, 30µ PP 300Å C4, 450g $1,602 EA PP-30C4/1250G PuriFlash Column, 30µ PP 300Å C4, 1250g $4,191 EA PP-30C4/2500G PuriFlash Column, 30µ PP 300Å C4, 2500g $8,290 EA PT-15C18T/6G PuriFlash Column, 15µ PT 200Å C18T, 6g $210 2/PK PT-15C18T/20G PuriFlash Column, 15µ PT 200Å C18T, 20g $224 EA PT-15C18T/35G PuriFlash Column, 15µ PT 200Å C18T, 35g $359 EA PT-15C18T/55G PuriFlash Column, 15µ PT 200Å C18T, 55g $565 EA PT-15C18T/120G PuriFlash Column, 15µ PT 200Å C18T, 120g $1,213 EA PT-15C18T/175G PuriFlash Column, 15µ PT 200Å C18T, 175g $1,776 EA PT-15C18T/300G PuriFlash Column, 15µ PT 200Å C18T, 300g $3,110 EA PT-15C18T/450G PuriFlash Column, 15µ PT 200Å C18T, 450g $4,800 EA PT-15C18T/1250G PuriFlash Column, 15µ PT 200Å C18T, 1250g $12,596 EA PT-15C18T/2500G PuriFlash Column, 15µ PT 200Å C18T, 2500g $25,150 EA PT-30C18T/6G PuriFlash Column, 30µ PT 200Å C18T, 6g $170 2/PK PT-30C18T/20G PuriFlash Column, 30µ PT 200Å C18T, 20g $170 EA PT-30C18T/35G PuriFlash Column, 30µ PT 200Å C18T, 35g $205 EA PT-30C18T/55G PuriFlash Column, 30µ PT 200Å C18T, 55g $245 EA PT-30C18T/120G PuriFlash Column, 30µ PT 200Å C18T, 120g $490 EA PT-30C18T/175G PuriFlash Column, 30µ PT 200Å C18T, 175g $598 EA PT-30C18T/300G PuriFlash Column, 30µ PT 200Å C18T, 300g $1,040 EA PT-30C18T/450G PuriFlash Column, 30µ PT 200Å C18T, 450g $1,602 EA PT-30C18T/1250G PuriFlash Column, 30µ PT 200Å C18T, 1250g $4,191 EA PT-30C18T/2500G PuriFlash Column, 30µ PT 200Å C18T, 2500g $8,290 EA

22 20 PuriFlash Reverse Phase Biopurification 200Å C8 & C4 Bonded Silica PT-15C8/6G PuriFlash Column, 15µ PT 200Å C8, 6g $210 2/PK PT-15C8/20G PuriFlash Column, 15µ PT 200Å C8, 20g $210 EA PT-15C8/35G PuriFlash Column, 15µ PT 200Å C8, 35g $330 EA PT-15C8/55G PuriFlash Column, 15µ PT 200Å C8, 55g $521 EA PT-15C8/120G PuriFlash Column, 15µ PT 200Å C8, 120g $1,119 EA PT-15C8/175G PuriFlash Column, 15µ PT 200Å C8, 175g $1,640 EA PT-15C8/300G PuriFlash Column, 15µ PT 200Å C8, 300g $2,868 EA PT-15C8/450G PuriFlash Column, 15µ PT 200Å C8, 450g $4,429 EA PT-15C8/1250G PuriFlash Column, 15µ PT 200Å C8, 1250g $11,621 EA PT-15C8/2500G PuriFlash Column, 15µ PT 200Å C8, 2500g $23,198 EA PT-30C8/6G PuriFlash Column, 30µ PT 200Å C8, 6g $170 2/PK PT-30C8/20G PuriFlash Column, 30µ PT 200Å C8, 20g $170 EA PT-30C8/35G PuriFlash Column, 30µ PT 200Å C8, 35g $205 EA PT-30C8/55G PuriFlash Column, 30µ PT 200Å C8, 55g $245 EA PT-30C8/120G PuriFlash Column, 30µ PT 200Å C8, 120g $490 EA PT-30C8/175G PuriFlash Column, 30µ PT 200Å C8, 175g $592 EA PT-30C8/300G PuriFlash Column, 30µ PT 200Å C8, 300g $1,028 EA PT-30C8/450G PuriFlash Column, 30µ PT 200Å C8, 450g $1,587 EA PT-30C8/1250G PuriFlash Column, 30µ PT 200Å C8, 1250g $4,144 EA PT-30C8/2500G PuriFlash Column, 30µ PT 200Å C8, 2500g $8,199 EA PT-15C4/6G PuriFlash Column, 15µ PT 200Å C4, 6g $210 2/PK PT-15C4/20G PuriFlash Column, 15µ PT 200Å C4, 20g $212 EA PT-15C4/35G PuriFlash Column, 15µ PT 200Å C4, 35g $342 EA PT-15C4/55G PuriFlash Column, 15µ PT 200Å C4, 55g $539 EA PT-15C4/120G PuriFlash Column, 15µ PT 200Å C4, 120g $1,154 EA PT-15C4/175G PuriFlash Column, 15µ PT 200Å C4, 175g $1,690 EA PT-15C4/300G PuriFlash Column, 15µ PT 200Å C4, 300g $2,954 EA PT-15C4/450G PuriFlash Column, 15µ PT 200Å C4, 450g $4,562 EA PT-15C4/1250G PuriFlash Column, 15µ PT 200Å C4, 1250g $11,971 EA PT-15C4/2500G PuriFlash Column, 15µ PT 200Å C4, 2500g $23,896 EA PT-30C4/6G PuriFlash Column, 30µ PT 200Å C4, 6g $170 2/PK PT-30C4/20G PuriFlash Column, 30µ PT 200Å C4, 20g $170 EA PT-30C4/35G PuriFlash Column, 30µ PT 200Å C4, 35g $205 EA PT-30C4/55G PuriFlash Column, 30µ PT 200Å C4, 55g $245 EA PT-30C4/120G PuriFlash Column, 30µ PT 200Å C4, 120g $490 EA PT-30C4/175G PuriFlash Column, 30µ PT 200Å C4, 175g $598 EA PT-30C4/300G PuriFlash Column, 30µ PT 200Å C4, 300g $1,040 EA PT-30C4/450G PuriFlash Column, 30µ PT 200Å C4, 450g $1,602 EA PT-30C4/1250G PuriFlash Column, 30µ PT 200Å C4, 1250g $4,191 EA PT-30C4/2500G PuriFlash Column, 30µ PT 200Å C4, 2500g $8,290 EA

23 PuriFlash Cellufine Biopurification Ion Exchange and Affinity 21 CF-90A500/7ML PuriFlash Column, 90µ Cellufine DEAE, 7ml $47 2/PK CF-90A500/27ML PuriFlash Column, 90µ Cellufine DEAE, 27ml $71 EA CF-90A500/45ML PuriFlash Column, 90µ Cellufine DEAE, 45ml $109 EA CF-90A500/68ML PuriFlash Column, 90µ Cellufine DEAE, 68ml $168 EA CF-90A500/145ML PuriFlash Column, 90µ Cellufine DEAE, 145ml $362 EA CF-90A500/215ML PuriFlash Column, 90µ Cellufine DEAE, 215ml $527 EA CF-90A500/375ML PuriFlash Column, 90µ Cellufine DEAE, 375ml $916 EA CF-90A500/575ML PuriFlash Column, 90µ Cellufine DEAE, 575ml $1,414 EA CF-90A500/1540ML PuriFlash Column, 90µ Cellufine DEAE, 1540ml $3,693 EA CF-90A500/3100ML PuriFlash Column, 90µ Cellufine DEAE, 3100ml $7,295 EA CF-90Q500/7ML PuriFlash Column, 90µ Cellufine QA, 7ml $53 2/PK CF-90Q500/27ML PuriFlash Column, 90µ Cellufine QA, 27ml $82 EA CF-90Q500/45ML PuriFlash Column, 90µ Cellufine QA, 45ml $127 EA CF-90Q500/68ML PuriFlash Column, 90µ Cellufine QA, 68ml $200 EA CF-90Q500/145ML PuriFlash Column, 90µ Cellufine QA, 145ml $427 EA CF-90Q500/215ML PuriFlash Column, 90µ Cellufine QA, 215ml $624 EA CF-90Q500/375ML PuriFlash Column, 90µ Cellufine QA, 375ml $1,084 EA CF-90Q500/575ML PuriFlash Column, 90µ Cellufine QA, 575ml $1,673 EA CF-90Q500/1540ML PuriFlash Column, 90µ Cellufine QA, 1540ml $4,373 EA CF-90Q500/3100ML PuriFlash Column, 90µ Cellufine QA, 3100ml $8,658 EA CF-90C500/7ML PuriFlash Column, 90µ Cellufine CM, 7ml $47 2/PK CF-90C500/27ML PuriFlash Column, 90µ Cellufine CM, 27ml $71 EA CF-90C500/45ML PuriFlash Column, 90µ Cellufine CM, 45ml $112 EA CF-90C500/68ML PuriFlash Column, 90µ Cellufine CM, 68ml $171 EA CF-90C500/145ML PuriFlash Column, 90µ Cellufine CM, 145ml $368 EA CF-90C500/215ML PuriFlash Column, 90µ Cellufine CM, 215ml $536 EA CF-90C500/375ML PuriFlash Column, 90µ Cellufine CM, 375ml $931 EA CF-90C500/575ML PuriFlash Column, 90µ Cellufine CM, 575ml $1,437 EA CF-90C500/1540ML PuriFlash Column, 90µ Cellufine CM, 1540ml $3,752 EA CF-90C500/3100ML PuriFlash Column, 90µ Cellufine CM, 3100ml $7,410 EA CF-75S4/7ML PuriFlash Column, 75µ Cellufine S4, 7ml $124 EA CF-75S4/27ML PuriFlash Column, 75µ Cellufine S4, 27ml $430 EA CF-75S4/45ML PuriFlash Column, 75µ Cellufine S4, 45ml $692 EA CF-75S4/68ML PuriFlash Column, 75µ Cellufine S4, 68ml $1,096 EA CF-75S4/145ML PuriFlash Column, 75µ Cellufine S4, 145ml $2,350 EA CF-75S4/215ML PuriFlash Column, 75µ Cellufine S4, 215ml $3,443 EA CF-75S4/375ML PuriFlash Column, 75µ Cellufine S4, 375ml $6,034 EA CF-75S4/575ML PuriFlash Column, 75µ Cellufine S4, 575ml $9,318 EA CF-75S4/1540ML PuriFlash Column, 75µ Cellufine S4, 1540ml ###### Quote EA CF-75S4/3100ML PuriFlash Column, 75µ Cellufine S4, 3100ml ###### Quote EA

24 22 PuriFlash Cellufine Biopurification Endotoxin Removal and Hydrophobic Interaction CF-90EL/7ML PuriFlash Column, 75µ Cellufine Etclean L, 7ml $153 EA CF-90EL/27ML PuriFlash Column, 75µ Cellufine Etclean L, 27ml $527 EA CF-90EL/45ML PuriFlash Column, 75µ Cellufine Etclean L, 45ml $851 EA CF-90EL/68ML PuriFlash Column, 75µ Cellufine Etclean L, 68ml $1,346 EA CF-90EL/145ML PuriFlash Column, 75µ Cellufine Etclean L, 145ml $2,892 EA CF-90EL/215ML PuriFlash Column, 75µ Cellufine Etclean L, 215ml $4,235 EA CF-90EL/375ML PuriFlash Column, 75µ Cellufine Etclean L, 375ml $7,427 EA CF-90EL/575ML PuriFlash Column, 75µ Cellufine Etclean L, 575ml ###### Quote EA CF-90EL/1540ML PuriFlash Column, 75µ Cellufine Etclean L, 1540ml ###### Quote EA CF-90EL/3100ML PuriFlash Column, 75µ Cellufine Etclean L, 3100ml ###### Quote EA CF-75ES/7ML PuriFlash Column, 75µ Cellufine Etclean S, 7ml $153 EA CF-75ES/27ML PuriFlash Column, 75µ Cellufine Etclean S, 27ml $527 EA CF-75ES/45ML PuriFlash Column, 75µ Cellufine Etclean S, 45ml $851 EA CF-75ES/68ML PuriFlash Column, 75µ Cellufine Etclean S, 68ml $1,346 EA CF-75ES/145ML PuriFlash Column, 75µ Cellufine Etclean S, 145ml $2,892 EA CF-75ES/215ML PuriFlash Column, 75µ Cellufine Etclean S, 215ml $4,235 EA CF-75ES/375ML PuriFlash Column, 75µ Cellufine Etclean S, 375ml $7,427 EA CF-75ES/575ML PuriFlash Column, 75µ Cellufine Etclean S, 575ml ###### Quote EA CF-75ES/1540ML PuriFlash Column, 75µ Cellufine Etclean S, 1540ml ###### Quote EA CF-75ES/3100ML PuriFlash Column, 75µ Cellufine Etclean S, 3100ml ###### Quote EA CF-90C4/7ML PuriFlash Column, 90µ Cellufine C4, 7ml $65 EA CF-90C4/27ML PuriFlash Column, 90µ Cellufine C4, 27ml $215 EA CF-90C4/45ML PuriFlash Column, 90µ Cellufine C4, 45ml $342 EA CF-90C4/68ML PuriFlash Column, 90µ Cellufine C4, 68ml $539 EA CF-90C4/145ML PuriFlash Column, 90µ Cellufine C4, 145ml $1,157 EA CF-90C4/215ML PuriFlash Column, 90µ Cellufine C4, 215ml $1,696 EA CF-90C4/375ML PuriFlash Column, 90µ Cellufine C4, 375ml $2,966 EA CF-90C4/575ML PuriFlash Column, 90µ Cellufine C4, 575ml $4,579 EA CF-90C4/1540ML PuriFlash Column, 90µ Cellufine C4, 1540ml ###### Quote EA CF-90C4/3100ML PuriFlash Column, 90µ Cellufine C4, 3100ml ###### Quote EA CF-90PH/7ML PuriFlash Column, 90µ Cellufine Phenyl, 7ml $65 EA CF-90PH/27ML PuriFlash Column, 90µ Cellufine Phenyl, 27ml $215 EA CF-90PH/45ML PuriFlash Column, 90µ Cellufine Phenyl, 45ml $342 EA CF-90PH/68ML PuriFlash Column, 90µ Cellufine Phenyl, 68ml $539 EA CF-90PH/145ML PuriFlash Column, 90µ Cellufine Phenyl, 145ml $1,157 EA CF-90PH/215ML PuriFlash Column, 90µ Cellufine Phenyl, 215ml $1,696 EA CF-90PH/375ML PuriFlash Column, 90µ Cellufine Phenyl, 375ml $2,966 EA CF-90PH/575ML PuriFlash Column, 90µ Cellufine Phenyl, 575ml $4,579 EA CF-90PH/1540ML PuriFlash Column, 90µ Cellufine Phenyl, 1540ml ###### Quote EA CF-90PH/3100ML PuriFlash Column, 90µ Cellufine Phenyl, 3100ml ###### Quote EA

25 PuriFlash Normal Phase Jumbo Packs HP: High Performance Silica 23 PF-15SIHP-JP/4G PuriFlash Column, 15µ Si HP, 4g $836 80/PK PF-15SIHP-JP/12G PuriFlash Column, 15µ Si HP, 12g $2,014 80/PK PF-15SIHP-JP/25G PuriFlash Column, 15µ Si HP, 25g $1,843 48/PK PF-15SIHP-JP/40G PuriFlash Column, 15µ Si HP, 40g $2,717 48/PK PF-15SIHP-JP/80G PuriFlash Column, 15µ Si HP, 80g $1,919 16/PK PF-15SIHP-JP/120G PuriFlash Column, 15µ Si HP, 120g $2,755 16/PK PF-15SIHP-JP/200G PuriFlash Column, 15µ Si HP, 200g $2,356 8/PK PF-15SIHP-JP/300G PuriFlash Column, 15µ Si HP, 300g $2,945 8/PK PF-15SIHP-JP/800G PuriFlash Column, 15µ Si HP, 800g $4,655 4/PK PF-15SIHP-JP/1600G PuriFlash Column, 15µ Si HP, 1600g $8,930 4/PK PF-30SIHP-JP/4G PuriFlash Column, 30µ Si HP, 4g $1, /PK PF-30SIHP-JP/12G PuriFlash Column, 30µ Si HP, 12g $1, /PK PF-30SIHP-JP/25G PuriFlash Column, 30µ Si HP, 25g $1, /PK PF-30SIHP-JP/40G PuriFlash Column, 30µ Si HP, 40g $2,014 80/PK PF-30SIHP-JP/80G PuriFlash Column, 30µ Si HP, 80g $1,577 40/PK PF-30SIHP-JP/120G PuriFlash Column, 30µ Si HP, 120g $1,710 32/PK PF-30SIHP-JP/200G PuriFlash Column, 30µ Si HP, 200g $1,520 16/PK PF-30SIHP-JP/300G PuriFlash Column, 30µ Si HP, 300g $2,413 16/PK PF-30SIHP-JP/800G PuriFlash Column, 30µ Si HP, 800g $1,691 4/PK PF-30SIHP-JP/1600G PuriFlash Column, 30µ Si HP, 1600g $2,983 4/PK PF-50SIHP-JP/4G PuriFlash Column, 50µ Si HP, 4g $1, /PK PF-50SIHP-JP/12G PuriFlash Column, 50µ Si HP, 12g $1, /PK PF-50SIHP-JP/25G PuriFlash Column, 50µ Si HP, 25g $1, /PK PF-50SIHP-JP/40G PuriFlash Column, 50µ Si HP, 40g $1,178 80/PK PF-50SIHP-JP/80G PuriFlash Column, 50µ Si HP, 80g $851 40/PK PF-50SIHP-JP/120G PuriFlash Column, 50µ Si HP, 120g $1,368 32/PK PF-50SIHP-JP/200G PuriFlash Column, 50µ Si HP, 200g $1,045 16/PK PF-50SIHP-JP/300G PuriFlash Column, 50µ Si HP, 300g $1,577 16/PK PF-50SIHP-JP/800G PuriFlash Column, 50µ Si HP, 800g $950 4/PK PF-50SIHP-JP/1600G PuriFlash Column, 50µ Si HP, 1600g $1,482 4/PK HC: High Capacity Silica PF-15SIHC-JP/4G PuriFlash Column, 15µ Si HC, 4G $836 80/PK PF-15SIHC-JP/12G PuriFlash Column, 15µ Si HC, 12G $2,014 80/PK PF-15SIHC-JP/25G PuriFlash Column, 15µ Si HC, 25G $1,843 48/PK PF-15SIHC-JP/40G PuriFlash Column, 15µ Si HC, 40G $2,717 48/PK PF-15SIHC-JP/80G PuriFlash Column, 15µ Si HC, 80G $1,919 16/PK PF-15SIHC-JP/120G PuriFlash Column, 15µ Si HC, 120g $2,755 16/PK PF-15SIHC-JP/200G PuriFlash Column, 15µ Si HC, 200g $2,356 8/PK PF-15SIHC-JP/300G PuriFlash Column, 15µ Si HC, 300g $2,945 8/PK PF-15SIHC-JP/800G PuriFlash Column, 15µ Si HC, 800g $4,655 4/PK PF-15SIHC-JP/1600G PuriFlash Column, 15µ Si HC, 1600g $8,930 4/PK PF-25SIHC-JP/4G PuriFlash Column, 25µ Si HC, 4g $1, /PK PF-25SIHC-JP/12G PuriFlash Column, 25µ Si HC, 12g $1, /PK PF-25SIHC-JP/25G PuriFlash Column, 25µ Si HC, 25g $1, /PK PF-25SIHC-JP/40G PuriFlash Column, 25µ Si HC, 40g $2,014 80/PK PF-25SIHC-JP/80G PuriFlash Column, 25µ Si HC, 80g $1,577 40/PK PF-25SIHC-JP/120G PuriFlash Column, 25µ Si HC, 120g $1,710 32/PK PF-25SIHC-JP/200G PuriFlash Column, 25µ Si HC, 200g $1,520 16/PK PF-25SIHC-JP/300G PuriFlash Column, 25µ Si HC, 300g $2,413 16/PK PF-25SIHC-JP/800G PuriFlash Column, 25µ Si HC, 800g $1,691 4/PK PF-25SIHC-JP/1600G PuriFlash Column, 25µ Si HC, 1600g $2,983 4/PK PF-50SIHC-JP/4G PuriFlash Column, 50µ Si HC, 4g $1, /PK PF-50SIHC-JP/12G PuriFlash Column, 50µ Si HC, 12g $1, /PK PF-50SIHC-JP/25G PuriFlash Column, 50µ Si HC, 25g $1, /PK PF-50SIHC-JP/40G PuriFlash Column, 50µ Si HC, 40g $1,216 80/PK PF-50SIHC-JP/80G PuriFlash Column, 50µ Si HC, 80g $1,273 40/PK PF-50SIHC-JP/120G PuriFlash Column, 50µ Si HC, 120g $1,406 32/PK PF-50SIHC-JP/200G PuriFlash Column, 50µ Si HC, 200g $1,178 16/PK PF-50SIHC-JP/300G PuriFlash Column, 50µ Si HC, 300g $1,615 16/PK PF-50SIHC-JP/800G PuriFlash Column, 50µ Si HC, 800g $988 4/PK PF-50SIHC-JP/1600G PuriFlash Column, 50µ Si HC, 1600g $2,014 4/PK

26 24 Reverse Phase, Linear Scale-up from Analytical to Flash Analytical, 250 x 4.6mm 5um C18 mau Area: Area: Area: Area: Area: :00 01:00 02:00 03:00 04:00 05:00 06:00 07:00 08:00 DAD1 A, Sig=215, 4 Ref=360,100 ( / D Area: Area: puriflash 15um C18, 35g Part number PF-15C18/35G :00 10:00 15:00 20:00 25:00 30:00 35:00 40:00 % Solvent: A: Water B: Acetonitrile C: Ammonium formate - 10% in 50/50 Acetonitrile/Water Flow Rate : Analytical 1.0, Flash 20 UV 254nm

27 PuriFlash Column Specifications Column Type 0012 Modify the 12g to make a 4g, see ID & Length Sorbent Weight ID (mm) Length (mm) Volume 4g Si, 6g Bonded ml 12g Si, 20g Bonded ml 25g Si, 35g Bonded ml 40g Si, 55g Bonded ml 80g Si, 120g Bonded ml 120g Si, 175g Bonded ml 200g Si, 300g Bonded ml 300g Si, 450g Bonded ml Max Pressure Typical Flow Rate 22 bar / 320 PSI 7 22 bar / 320 PSI 22 bar / 320 PSI 20 bar / 290 PSI 20 bar / 290 PSI 20 bar / 290 PSI 20 bar / 290 PSI 20 bar / 290 PSI Flow Range g Si, 1250g Bonded ml 7 bar / 100 PSI Coming Soon 1600g Si, 2500g Bonded ml 2500g Si, 3750g Bonded 4850 ml 7 bar / 100 PSI 7 bar / 100 PSI info@interchiminc.com Interchim Inc. All prices subject to change

28 puriflash Column Loading Chart Solvent Front 1600 Rf (B ) 800 Rf (A) 300 rigin CV (A) = 1/ Rf (A) CV (B) = 1/ Rf (B) ΔCV = CV (A) CV (B) ΔCV ΔCV= 1 1,5 Difficult ΔCV= 1,5 4 Typical ΔCV= 4 10 Easy Sample size (mg) PURIFICATIN INSTRUMENTATIN FR ALL Interchim offers the most comprehensive range of instruments from Flash to preparative HPLC Column size (g) HPLC pumps for reliability Detection with UV, ELSD Same software for Flash & HPLC Flash, Evo Series 150, 150 PSI 200, 435 PSI 250, 750 PSI 800, 150 PSI 19" x 17" x 26" Prep HPLC, Spot II 50, 4350 PSI 200, 3300 PSI 500, 1600 PSI 24" x 23" x 26" info@interchiminc.com This chart is based on using 50µ HC silica columns under normal phase conditions. Expect two to three times higher loading with 25µ and 15µ silica. For reverse phase and ion exchange loading refer to pages 3-6.