Electrophoresis File Run Summary

Transcription

1 2100 expert DE _ _ xad Page 1 of 19 C:\..._DE _ _ xad Electrophoresis File Run Summary Instrument Information: Instrument Name: DE Firmware: C Serial#: Assay Information: Assay Origin Path: DE Type: G2939A C:\Program Files\Agilent\2100 bioanalyzer\2100 expert\assays\rna\ Series II.xsy Version: Assay Comments: Chip Information: Chip Lot #: Reagent Kit Lot #: Chip Comments: 2.6 Total RNA Analysis ng sensitivity (Eukaryote) Copyright Agilent Technologies, Inc. H5N RIN: 6.20 RIN: 6.90 RIN: RIN: 7.40 RIN N/A RIN: RIN:7 RIN: 7.30 RIN: RIN: 7.50 RIN: 7.20 RIN: 7.10

2 2100 expert DE _ _ xad Page 2 of 19 C:\..._DE _ _ xad Electrophoresis File Run Summary (Chip Summary) Sample Name Sample Comment Status Result Label Result Color H5N RIN: RIN: RIN: RIN: RIN N/A 171 RIN: RIN:7 173 RIN: RIN: RIN: RIN: RIN: 7.10 Ladder All Other Samples Chip Lot # Reagent Kit Lot # Chip Comments :

3 2100 expert DE _ _ xad Page 3 of 19 General Analysis Settings Number of Available Sample and Ladder Wells (Max.) : 13 Minimum Visible Range [s] : 17 Maximum Visible Range [s] : 70 Start Analysis Time Range [s] : 19 End Analysis Time Range [s] : 69 Ladder Concentration [ng/µl] : 150 Lower Marker Concentration [ng/µl] : 0 Upper Marker Concentration [ng/µl] : 0 Used Lower Marker for Quantitation Standard Curve Fit is Logarithmic Show Data Aligned to Lower Marker Integrator Settings Integration Start Time [s] : 19 Integration End Time [s] : 69 Slope Threshold : 0.6 Height Threshold [FU] : 0.5 Area Threshold : 0.2 Width Threshold [s] : 0.5 Baseline Plateau [s] : 6 Filter Settings Filter Width [s] : 0.5 Polynomial Order : 4 Ladder Ladder Peak Size C:\..._DE _ _ xad Electrophoresis Assay Details

4 2100 expert DE _ _ xad Page 4 of 19 C:\..._DE _ _ xad Electropherogram Summary Ladder Overall Results for Ladder RNA Area: ng/µl Result Flagging Label: All Other Samples

5 2100 expert DE _ _ xad Page 5 of 19 C:\..._DE _ _ xad H5N Overall Results for sample 1 : H5N RNA Area: ng/µl rrna Ratio [28s / 18s]: (B.02.08) Result Flagging Label: RIN: 6.20 Fragment table for sample 1 : H5N S S

6 2100 expert DE _ _ xad Page 6 of 19 C:\..._DE _ _ xad 167 Overall Results for sample 2 : 167 RNA Area: ng/µl rrna Ratio [28s / 18s]: (B.02.08) Result Flagging Label: RIN: 6.90 Fragment table for sample 2 : S S

7 2100 expert DE _ _ xad Page 7 of 19 C:\..._DE _ _ xad 168 Overall Results for sample 3 : 168 RNA Area: ng/µl rrna Ratio [28s / 18s]: (B.02.08) Result Flagging Label: RIN: 7.40 Fragment table for sample 3 : S S

8 2100 expert DE _ _ xad Page 8 of 19 C:\..._DE _ _ xad 169 Overall Results for sample 4 : 169 RNA Area: ng/µl rrna Ratio [28s / 18s]: (B.02.08) Result Flagging Label: RIN: 7.40 Fragment table for sample 4 : S S

9 2100 expert DE _ _ xad Page 9 of 19 C:\..._DE _ _ xad 170 Overall Results for sample 5 : 170 RNA Area: ng/µl rrna Ratio [28s / 18s]: 2.4 N/A (B.02.08) Result Flagging Label: RIN N/A Fragment table for sample 5 : S S

10 2100 expert DE _ _ xad Page 10 of 19 C:\..._DE _ _ xad 171 Overall Results for sample 6 : 171 RNA Area: ng/µl rrna Ratio [28s / 18s]: (B.02.08) Result Flagging Label: RIN: 7.40 Fragment table for sample 6 : S S

11 2100 expert DE _ _ xad Page 11 of 19 C:\..._DE _ _ xad 172 Overall Results for sample 7 : 172 RNA Area: ng/µl rrna Ratio [28s / 18s]: 1.7 Result Flagging Label: 7 (B.02.08) RIN:7 Fragment table for sample 7 : S S

12 2100 expert DE _ _ xad Page 12 of 19 C:\..._DE _ _ xad 173 Overall Results for sample 8 : 173 RNA Area: ng/µl rrna Ratio [28s / 18s]: (B.02.08) Result Flagging Label: RIN: 7.30 Fragment table for sample 8 : S S

13 2100 expert DE _ _ xad Page 13 of 19 C:\..._DE _ _ xad 174 Overall Results for sample 9 : 174 RNA Area: ng/µl rrna Ratio [28s / 18s]: (B.02.08) Result Flagging Label: RIN: 7.30 Fragment table for sample 9 : S S

14 2100 expert DE _ _ xad Page 14 of 19 C:\..._DE _ _ xad 175 Overall Results for sample 10 : 175 RNA Area: ng/µl rrna Ratio [28s / 18s]: (B.02.08) Result Flagging Label: RIN: 7.50 Fragment table for sample 10 : S S

15 2100 expert DE _ _ xad Page 15 of 19 C:\..._DE _ _ xad 178 Overall Results for sample 11 : 178 RNA Area: ng/µl rrna Ratio [28s / 18s]: (B.02.08) Result Flagging Label: RIN: 7.20 Fragment table for sample 11 : S S

16 2100 expert DE _ _ xad Page 16 of 19 C:\..._DE _ _ xad 179 Overall Results for sample 12 : 179 RNA Area: ng/µl rrna Ratio [28s / 18s]: (B.02.08) Result Flagging Label: RIN: 7.10 Fragment table for sample 12 : S S

17 2100 expert DE _ _ xad Page 17 of 19 Gel Image C:\..._DE _ _ xad

18 2100 expert DE _ _ xad Page 18 of 19 Curves C:\..._DE _ _ xad Standard Curve

19 2100 expert DE _ _ xad Page 19 of 19 Run Logbook C:\..._DE _ _ xad Description Number Source Category Sub Category Time Time Zone User Host Run ended on port 1 (Number of wells acquired: 13) Run started on port 1 (File: C:\Program Files\Agilent\21 00 bioanalyzer\210 0 expert\data\ \2100 expert_eukaryot e Total RNA Nano_DE _ _ xad) Instrument Run 4/14/ :31:38 PM Instrument Run 4/14/ :07:56 PM (GMT --04:00) Eastern Standard Time (GMT --04:00) Eastern Standard Time zhouhu zhouhu NIH NIH Product Number : G2939A Instrument Run 4/14/ :07:56 PM Name : Instrument Run 4/14/ :07:56 PM Vendor : Agilent Technologies Serial# : DE Firmware : C Cartridge : Electrode Instrument Run 4/14/ :07:56 PM Instrument Run 4/14/ :07:56 PM Instrument Run 4/14/ :07:56 PM Instrument Run 4/14/ :07:56 PM (GMT --04:00) Eastern Standard Time (GMT --04:00) Eastern Standard Time (GMT --04:00) Eastern Standard Time (GMT --04:00) Eastern Standard Time (GMT --04:00) Eastern Standard Time (GMT --04:00) Eastern Standard Time zhouhu zhouhu zhouhu zhouhu zhouhu zhouhu NIH NIH NIH NIH NIH NIH