Annex to the Accreditation Certificate D-PL according to DIN EN ISO/IEC 17025:2005
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1 Deutsche Akkreditierungsstelle GmbH Annex to the Accreditation Certificate D-PL according to DIN EN ISO/IEC 17025:2005 Period of validity: to Holder of certificate: Institut Kuhlmann GmbH Analytik-Zentrum Ludwigshafen Hedwig-Laudien-Ring 3, Ludwigshafen Tests in the fields: physical, physico-chemical and chemical investigations of dietetic food and nutritional supplements in powder and liquid form; physico-chemical investigations of utensils; sampling of water, ground water and bathing water; sensory, physical, physico-chemical, chemical and microbiological investigations of water, ground water, bathing water and waste water; sampling and investigations in accordance with the Drinking Water Ordinance, with the exception of radioactive parameters Abbreviations used: see last page Within the given testing field marked with *, the testing laboratory is permitted, without being required to inform and obtain prior approval from DAkkS, the free choice of standard or equivalent testing methods. Within the given testing field marked with **, the testing laboratory is permitted, without being required to inform and obtain prior approval from DAkkS, the modification, development and refinement of testing methods. This document is a translation. The definitive version is the original German annex to the accreditation certificate. 1/17
2 Within the scope of accreditation marked with ***, the testing laboratory is permitted, without being required to inform and obtain prior approval from DAkkS, to use standard testing methods listed here with different issue dates. The listed testing methods are exemplary. The testing laboratory maintains a current list of all testing methods within the flexible scope of accreditation. 1 Dietetic food and nutritional supplements in powder and liquid form 1.1 Physical, physico-chemical and chemical investigations of dietetic food and nutritional supplements in powder and liquid form Determination of mineral substances, trace elements and contaminants by atomic absorption spectrometry *** ASU L / Foodstuffs - Determination of trace elements in foodstuffs; Part 2: Determination of iron, copper, manganese and zinc by flame atomic absorption spectroscopy (AAS) (Deviation: also sodium, potassium, calcium and magnesium) Determination of mineral compounds, trace elements and contaminants by mass spectrometry with inductively coupled plasma ** ASU L / ASU L In house method SO Foodstuffs - Determination of trace elements; Part 3: Determination of lead, cadmium, chromium and molybdenum by graphite furnace atomic absorption spectroscopy (GFAAS) after pressure digestion (Deviation: also further elements, ICP/MS) Investigation of foodstuffs - Determination of iodine in dietetic foodstuffs with ICP-MS (mass spectrometry with inductively coupled plasma) Screening of the element spectrum in foodstuffs and various products by ICP-MS Titrimetric determination of contents and additives * ASU L / Investigation of foodstuffs - Determination of nitrogen content in milk according to Kjeldahl and calculation of raw protein content (Deviation: only dietetic foodstuffs and nutritional supplements in powder and liquid form) Period of validity: to Translation - 2/17
3 ASU L Ph. Eur th edition Investigation of foodstuffs - Determination of the peroxide number in fats and oils (Wheeler method) (Deviation: also in dietetic foodstuffs and nutritional supplements in powder and liquid form following cold extraction) Water semi-micro determination by Karl Fischer titration Electrochemical determination of contents and additives * ASU L IFU Investigation of foodstuffs - Determination of fluoride in natural mineral water (Deviation: following Schöninger incineration or incineration with Ca(OH) 2 also for foodstuff samples) Determination of ph Value Gravimetric determination of contents and additives * ASU L ASU L ASU L Investigation of foodstuffs - Determination of the fat content in milk and milk products; Weibull method (Deviation: only dietetic foodstuffs and nutritional supplements in powder and liquid form) Investigation of foodstuffs - Determination of the mass of dry matter in meat and meat products (Deviation: only dietetic foodstuffs and nutritional supplements in powder and liquid form) Investigation of foodstuffs - Determination of the ash in meat and meat products (Deviation: only dietetic foodstuffs and nutritional supplements in powder and liquid form) Photometric determination of contents and additives * ASU L Investigation of foodstuffs - Determination of total phosphorus content in meat and meat products (Deviation: only dietetic foodstuffs and nutritional supplements in powder and liquid form) Period of validity: to Translation - 3/17
4 ASU L SOP LM Investigation of foodstuffs - Determination of starch content in meat products (enzymatic) (Deviation: only dietetic foodstuffs and nutritional supplements in powder and liquid form) Photometric determination of chondroitin sulfate in dietetic foodstuffs and nutritional supplements in powder and liquid form Determination of amino acids with the amino acid analyser ** ASU L ASU L ASU L In house method AS 02 to to In house method AS In house method AS Investigation of foodstuffs - Determination of the amino acids content in amino acid mixtures Investigation of foodstuffs - Protein hydrolysate - based determination of amino acid content in dietetic foodstuffs Investigation of foodstuffs - Protein hydrolysate - based determination of tryptophane content in dietetic foodstuffs Determination of amino acids in products containing proteins following different hydrolyses with the amino acid analyser Determination of cysteine with the amino acid analyser (foodstuff matrix) Determination of taurine in foodstuffs and animal feeds Determination of contents, additives and residues by high-pressure liquid chromatography with standard detectors ** ASU L ASU L ASU L Investigation of foodstuffs - Determination of acesulfame K, aspartame and saccharine sodium in foodstuffs (Deviation: also sodium cyclamate and salicylic acid) Investigation of foodstuffs - Determination of isomalt in foodstuffs Investigation of foodstuffs - Determination of vitamin D (cholecalciferol (D 3) and ergocalciferol (D 2)) in foodstuffs by HPLC (Deviation: HPLC-MS) Period of validity: to Translation - 4/17
5 ASU L ASU L ASU L ASU L Swiss LMB 62/ Swiss LMB Swiss LMB Swiss LMB Swiss LMB DGF F II 4a (00) 2000 In house method VIT 04 c In house method VIT In house method VIT In house method VIT Investigation of foodstuffs - Determination of sugar alcohols (lactitol, maltitol, xylitol, sorbitol and mannitol) in foodstuffs Investigation of foodstuffs - Determination of saccharide contents: fructose, glucose, sucrose, turanose and maltose - HPLC method Investigation of foodstuffs - Investigation of coffee and coffee products - Determination of caffein content (HPLC fast procedure) (Deviation: only dietetic foodstuffs and nutritional supplements in powder and liquid form) Investigation of foodstuffs - Determination of vitamin A in dietetic foodstuffs by HPLC with UV detector (Deviation: also vitamin E) Determination of carotenoids in foodstuffs by HPLC-DAD Determination of vitamin K 1 in foodstuffs by HPLC with fluorescence detector Determination of vitamin B 1 in foodstuffs by HPLC with fluorescence detector Determination of vitamin B 2 in foodstuffs by HPLC with fluorescence detector Determination of ascorbic acid in foodstuffs by HPLC with UV detector Determination of free tocopherols and tocotrienols in oils by HPLC with fluorescence detector Determination of lycopene in foodstuffs by HPLC-DAD Determination of dehydroascorbic acid in foodstuffs by HPLC with UV detector Determination of lutein in foodstuffs by HPLC-DAD Determination of vitamin A palmitate, vitamin A acetate and ascorbyl palmitate by HPLC-DAD Period of validity: to Translation - 5/17
6 In house method LC In house method LC Determination of coenzyme Q 10 by HPLC-DAD Determination of soya isoflavones (daidzein and genistein) in foodstuffs by HPLC-DAD Determination of contents and additives by high-pressure liquid chromatography with mass spectrometry ** In house method VIT 33Q In house method VIT 17Q In house method VIT 20Q In house method VIT 01Q In house method LC In house method LC 30Q In house method LC 39Q In house method LC Multi-method B-vitamins by HPLC-MS-MS (foodstuff matrix) Determination of biotin in foodstuffs by HPLC-MS-MS Determination of folic acid in foodstuffs by HPLC-MS-MS Determination of vitamin B 12 in foodstuffs by HPLC-MS-MS Determination of choline in foodstuffs by HPLC-MS Determination of carnitine in foodstuffs by HPLC-MS-MS Determination of nucleotides in foodstuffs by HPLC-MS-MS (foodstuff matrix) Determination of glucosamine sulfate in foodstuffs by HPLC-MS (foodstuff matrix) Investigation of contents by gas chromatography with standard detectors * ASU L 22.02/ Investigation of foodstuffs - Determination of cholesterin content in egg-based farinaceous products - gas-chromatographic method (Deviation: also GC/MS, only dietetic foodstuffs and nutritional supplements in powder and liquid form) Period of validity: to Translation - 6/17
7 DGF C-VI 11d 1998 Determination of fatty acid composition in oils and fats following preparation of the fatty acid methyl esters (Deviation: also in dietetic foodstuffs and nutritional supplements in powder and liquid form following extraction of fat) Determination of contents and additives by HPAEC (PAD and EC) ** AOAC Official Method In house method LM In house method LM In house method LM Trans-galactooligosaccharides (TGOS) via galactose/ lactose by HPAEC-PAD Net carbohydrates, free sugars and sugar alcohols by HPAEC-PAD Oligofructose (inulin) via fructose/sucrose By HPAEC-PAD Chloride, nitrate and nitrite in dietetic foodstuffs and nutritional supplements in powder and liquid form by HPAEC-CD 2 Utensils 2.1 Physico-chemical investigations of utensils Determination of migration contaminants from utensils and packaging material by gas chromatography with mass spectrometry ** In house method SO In house method SO Investigations of migration from packaging material by HPLC-MS and GC-MS (here: GC-MS) Acrylates as acrylic acid in migrates by Headspace-GC-MS Determination of migration contaminants from utensils by high-pressure liquid chromatography - mass spectrometry ** In house method SO Investigations of migration from packaging material by HPLC-MS and GC-MS (here: HPLC-MS) Period of validity: to Translation - 7/17
8 3 Investigations of water, ground water, bathing water and waste water *** 3.1 Sampling DIN EN ISO (A 4) ISO DIN A DIN ISO (A 14) DIN EN ISO (A 21) DIN EN ISO (K 19) DIN Notification of the UBA BGesundhBl DVWK LUBW Landesanstalt für Umwelt, Messungen und Naturschutz Baden- Württemberg Water quality - Sampling - Part 1: Guidance on the design of sampling programmes and sampling techniques Water quality - Sampling - Part 11: guidance on sampling of groundwaters sampling from aquifers Water quality - Sampling - Part 5: Guidance on sampling of drinking water from treatment works and piped distribution systems Water quality - Sampling - Part 3: Preservation and handling of water samples Water quality - Sampling for microbiological analysis Treatment of water of swimming pools and baths Hygiene requirements and their monitoring for bathing facilities Taking of ground water samples and scope of testing (bulletin withdrawn) Ground water monitoring programme - Baden-Württemberg - Guidelines for ground water sampling 3.2 Sensory investigations DEV B 1/ Testing of odour and flavour Period of validity: to Translation - 8/17
9 DIN EN 1622 (B 3) Water quality - Determination of the threshold odour number (TON) and threshold flavour number (TFN) 3.3 Physical and physico-chemical parameters DIN EN ISO 7887 (C 1) DIN EN ISO 7027 (C 2) DIN C DIN C DIN EN ISO (C 5) DIN C DIN EN (C 8) Water quality - Examination and determination of colour Water quality - Determination of turbidity Determination of absorption in the range of the ultraviolet radiation, Spectral absorptions coefficient Determination of temperature Water quality - Determination of ph Determination of the oxidation-reduction potential Water quality; determination of electrical conductivity 3.4 Anions DIN D DIN EN (D 10) DIN EN ISO 6878 (D 11) DIN D DIN D Determination of fluoride Water quality; Determination of nitrite; Molecular absorption spectrometric method Water quality - Determination of phosphorus - Ammonium molybdate spectrometric method Determination of cyanides Determination of readily liberated cyanides Period of validity: to Translation - 9/17
10 DIN EN ISO (D 20) DIN D DIN D DIN D DIN D DIN EN ISO (D 34) Water quality - Determination of dissolved anions by liquid chromatography of ions - Part 1: Determination of bromide, chloride, fluoride, nitrate, nitrite, phosphate and sulfate Determination of dissolved silicate by spectrometry Photometric determination of chromium(vi) using 1,5-diphenyl carbazide Spectrometric determination of dissolved sulfide Determination of easily liberatable sulfide Water quality - Determination of dissolved bromate - Method by liquid chromatography of ions 3.5 Cations DIN E DIN EN ISO (E 12) DIN EN ISO (E 29) Determination of ammonia-nitrogen Water quality - Determination of mercury - Method using atomic absorption spectrometry (AAS) with and without enrichment Water quality - Application of inductively coupled plasma mass spectrometry (ICP-MS) - Part 2: Determination of 62 elements and determination of iron and mercury 3.6 Collectively determined substances DIN EN ISO 6468 (F 1) DIN F DIN EN (F 15) Water quality - Determination of certain organochlorine insecticides, polychlorine biphenyls and chlorobenzenes - Gaschromatographic method after liquid-liquid extraction Determination of polychlorinated biphenyls Water quality - Gas chromatographic determination of some selected chlorophenols in water Period of validity: to Translation - 10/17
11 DIN F DIN F DIN F DIN F In house method PSM In house method HPLC In house method HPLC In house method HPLC In house method HPLC Determination of trihalogenmethanes in bathing water and pool water with headspace-gas chromatography Determination of selected polycyclic aromatic hydrocarbons (PAH) - Method using gas chromatography with mass spectrometric detection (GC-MS) Determination of selected polyfluorinated compounds (PFC) in water - Method using high performance liquid chromatography and mass spectrometric detection (HPLC/MS-MS) after solid-liquid extraction Determination of selected easily volatile organic compounds in water - Method using gas chromatography and mass spectrometry by static headspace technique (HS-GC-MS) Pesticides by LC-MS-MS - Determination of selected active substances and metabolites in pesticides by high-performance liquid chromatography method with mass spectrometric detection (HPLC-MS/MS) Residual medicines by LC-QTOF - High-performance liquid chromatography method with mass spectrometric detection (HPLC-QTOF) Glyphosate by LC-MS-MS - High-performance liquid chromatography method with mass spectrometric detection (HPLC-MS/MS) Sweeteners and benzotriazols by LC-MS-MS - High-performance liquid chromatography method with mass spectrometric detection (HPLC-MS/MS) X-ray contrast medium by LC-MS-MS - High-performance liquid chromatography method with mass spectrometric detection (HPLC-MS/MS) 3.7 Single components and gaseous components DIN EN ISO (G 4-2) Water quality - Determination of free chlorine and total chlorine - Part 2: Colorimetric method using N,N-diethyl-1,4- phenylenediamine, for routine control purposes Period of validity: to Translation - 11/17
12 DIN EN ISO 5814 (G 22) DIN G Water quality - Determination of dissolved oxygen - Electrochemical probe method Determination of oxygen saturation index 3.8 Overall parameters characterising effects and substances DIN H DIN H DIN EN 1484 (H 3) DIN EN ISO 8467 (H 5) DIN H DIN H DIN EN ISO 9562 (H 14) DIN H DIN H DIN H Suggested method DEV-H DIN EN (H 34) Determination of the total solids residue, the filtrate solids residue and the residue on ignition Quantitative determination of paper-filterable substances and ignition residue Water analysis - Guidelines for the determination of total organic carbon (TOC) and dissolved organic carbon (DOC) Water quality - Determination of permanganate index Determination of acid and base capacity Determination of the settleable matter by volume in water and waste water with a sample volume of 2 litres Water quality - Determination of adsorbable organically bound halogens (AOX) Photometric determination of the phenol index with 4-aminoantipyrine without distillation, with colour extraction Photometric determination of the phenol index with 4-aminoantipyrine following distillation and colour extraction Photometric determination of the phenol index with 4-aminoantipyrine following distillation, without colour extraction Determination of purgeable organically bound halogens (POX) Water quality - Determination of nitrogen - Determination of bound nitrogen (TNb), following oxidation to nitrogen oxides Period of validity: to Translation - 12/17
13 DIN ISO (H 45) DIN EN (H 51) DIN H Water quality - Determination of the chemical oxygen demand index (ST-COD) - Small-scale sealed tube method Water quality - Determination of biochemical oxygen demand after n days (BODn) - Part 1: Dilution and seeding method with allylthiourea addition Gravimetric determination of low volatile lipophilic substances after solvent extraction 3.9 Microbiological methods DIN EN ISO 6222 (K 5) DIN EN ISO (K 6-1) DIN EN ISO (K 11) DIN EN ISO (K 12) DIN EN ISO (K 13) DIN EN ISO (K 14) DIN EN ISO (K 15) TrinkwV 2001, Annex 5, Part I, d) bb) TrinkwV 2001, Annex 5, Part I, e) DIN EN ISO (K 17) Water quality - Enumeration of culturable micro-organisms - Colony count by inoculation in a nutrient agar culture medium Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 2: Most probable number method Water quality - Detection and enumeration of Pseudomonas aeruginosa - Method by membrane filtration Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora Water quality - Detection and enumeration of Escherichia coli and coliform bacteria in surface and waste water - Part 3: Miniaturized method (most probable number) by inoculation in liquid medium Water quality - Detection and enumeration of intestinal enterococci in surface and waste water - Part 1: Miniaturized method (most probable number) by inoculation in liquid medium Water quality - Detection and enumeration of intestinal enterococci - Part 2: Membrane filtration method Determination of cultivatable microorganisms - Colony count at 22 C and 36 C Detection of Clostridium perfringens (including spores) by membrane filtration (mcp method) Water quality - Detection and enumeration of bacteriophages - Part 2: Enumeration of somatic coliphages Period of validity: to Translation - 13/17
14 DIN EN ISO 8199 (K 20) DIN EN ISO (K 22) ISO UBA Recommendation Water quality - General guidance on the enumeration of microorganisms by culture Water quality - Detection and enumeration of Legionella - Part 2: Direct membrane filtration method for waters with low bacterial counts Water quality - Enumeration of Clostridium perfringens - Method using membrane filtration Systemic investigation of drinking water systems for Legionella according to Drinking Water Ordinance 4 Investigations in accordance with the Drinking Water Ordinance Sampling Method DIN ISO (A 14) DIN EN ISO (A 21) DIN EN ISO (K 19) DIN A DVWK LAWA AQS bulletin P-8/ Title Water quality - Sampling - Part 5: Guidance on sampling of drinking water from treatment works and piped distribution systems Water quality - Sampling - Part 3: Preservation and handling of water samples Water quality - Sampling for microbiological analysis sampling from aquifers (A 13) Taking of ground water samples and scope of testing Sampling of ground water ANNEX 1: MICROBIOLOGICAL PARAMETERS PART I: General requirements for drinking water No. Parameter Method 1 Escherichia coli (E. coli) DIN EN ISO (K12) Colilert -18/Quant-Tray 2 Enterococci DIN EN ISO (K15) PART II: Requirements for drinking water for dispensing from closed containers No. Parameter Method 1 Escherichia coli (E. coli) DIN EN ISO (K12) Enterococci DIN EN ISO (K15) Period of validity: to Translation - 14/17
15 No. Parameter Method 3 Pseudomonas aeruginosa DIN EN ISO (K11) ANNEX 2: CHEMICAL PARAMETERS PART I: Chemical parameters for which the concentrations in the distribution network, including the drinking water installation, as a rule no longer increase No. Parameter Method 1 Acrylamide not documented 2 Benzene DIN F Boron DIN EN ISO (E29) Bromate DIN EN ISO (D34) Chromium DIN EN ISO (E29) Cyanide DIN D ,2-dichlorethane DIN F Fluoride DIN EN ISO (D20) Nitrate DIN EN ISO (D 20) Active agents of pesticides and biocidal products In house method PSM 05 - Pesticides by LC-MS-MS 11 Total active agents of pesticides and biocidal products In house method PSM 05 - Pesticides by LC-MS-MS 12 Mercury DIN EN ISO (E12) DIN EN ISO (E29) Selenium DIN EN ISO (E29) Tetrachloroethene and trichloroethene DIN F Uranium DIN EN ISO (E29) PART II: Chemical parameters for which the concentrations in the distribution network, including the drinking water installation, can increase No. Parameter Method 1 Antimony DIN EN ISO (E29) Arsenic DIN EN ISO (E29) Benzo-(a)-pyrene DIN F Lead DIN EN ISO (E29) Cadmium DIN EN ISO (E29) Epichlorohydrin not documented 7 Copper DIN EN ISO (E29) Nickel DIN EN ISO (E29) Nitrite DIN EN (D10) Polycyclic aromatic hydrocarbons DIN F Trihalomethanes DIN F Period of validity: to Translation - 15/17
16 No. Parameter Method 12 Vinyl chloride DIN F ANNEX 3: INDICATOR PARAMETERS PART I: General indicator parameters No. Parameter Method 1 Aluminium DIN EN ISO (E29) Ammonium DIN E Chloride DIN EN ISO (D20) Clostridium perfringens (including spores) TrinkwV 2001 Annex 5 l e) 5 Coliform bacteria DIN EN ISO (K12) Colilert -18/Quanti-Tray 6 Iron DIN EN ISO (E29) Colour (spectral absorption coefficient Hg 436 nm) DIN EN ISO 7887 (C1) Odour DEV B1/2 Teil a Flavour DEV B1/2 Teil a Colony count at 22 C TrinkwV 2001 Annex 5 l d) bb) 11 Colony count at 36 C TrinkwV 2001 Annex 5 l d) bb) 12 Electrical conductivity DIN EN (C8) Manganese DIN EN ISO (E29) Sodium DIN EN ISO (E29) Organically bound carbon (TOC) DIN EN 1484 (H3) Oxidisability DIN EN ISO 8467 (H5) Sulfate DIN EN ISO (D 20) Turbidity DIN EN ISO 7027 (C2) Hydrogen ion concentration DIN EN ISO (C5) Calcite solubility DIN C (calculation method 3) 21 Tritium not documented 22 Total indicative dose not documented PART II: Special requirements for drinking water in drinking water installation facilities Legionella spec. Parameter Method ISO , DIN EN ISO (K22) UBA Recommendation Period of validity: to Translation - 16/17
17 Parameters not included in Annex 1 to 3 of the Drinking Water Ordinance of 2001 Further periodic investigations Parameter Method Calcium DIN EN ISO (E29) Potassium DIN EN ISO (E29) Magnesium DIN EN ISO (E29) Acid capacity DIN H The accreditation does not replace the validation or certification process requirement of the relevant authority in accordance with Article 15, Paragraph 4 of the Drinking Water Ordinance. Abbreviations used: ASU BGesundhBl. DEV DGF DIN DVWG DVWK EN EPA IEC IFU ISO LFBG LUBW Schweizer LMB TrinkwV UBA Amtliche Sammlung von Untersuchungsmethoden nach 64 LFGB (Official compendium of test methods according to Article 64 of the German Food and Feed Code) Bundesgesundheitsblatt (Federal Health Gazette) Deutsche Einheitsverfahren (German standard method) Deutsche Gesellschaft für Fettwissenschaft (German Society of Fats Science) Deutsches Institut für Normung (German Institute for Standardization) Deutscher Verein des Gas- und Wasserfachs e.v. (Association of the German Gas and Water Supply Industry) Deutscher Verband für Wasserwirtschaft und Kulturbau (German Association for Water Management and Landscape Ecology) Europäische Norm (European Standard) Environmental Protection Agency, USA International Electrotechnical Commission Internationale Fruchtsaft-Union (International Federation of Fruit Juice Producers) International Organization for Standardization Lebensmittel-, Futtermittel- und Bedarfsgegenstände-Gesetzbuch (German Food and Feed Code) Landesanstalt für Umwelt, Messungen und Naturschutz Baden-Württemberg (Environmental Ministry of Baden-Württemberg) Schweizer Lebensmittelbuch (Swiss Foodstuffs Code) Trinkwasserverordnung (Drinking Water Ordinance) Umweltbundesamt (Federal Environmental Agency) Period of validity: to Translation - 17/17
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