Biodiesel Analysis Toolkit

Transcription

1 Biodiesel Analysis Toolkit Sample Preparation, Methods and Analysis

2 Introduction Biodiesel is taking its place as one of the new cleaner and greener solutions required to meet the world s growing energy needs. New feedstocks, production techniques and applications are continuing to drive the production capabilities of Biodiesel to meet consumer demand, and this is therefore increasing the analysis required by Biodiesel producers. SGE is pleased to take our part in supporting the drive towards a sustainable future and we are available to assist you achieve your analytical requirements. This guide has been created as a resource for Biodiesel analysis. Inside you will find method information, products and solutions designed to ensure you can effectively and reliably complete fuels testing according to either ASTM International or European National (EN) methods. The latest information about our products and applications can always be found at our website About SGE SGE Analytical Science began manufacturing syringes over 5 years ago and now manufactures a vast array of products for chromatography and mass spectrometry. SGE is an ISO accredited company and is proud that all major analytical science instrument manufacturers rely on and incorporate SGE manufactured components in their systems. SGE is heavily committed to ongoing research and development that will allow the company to continue to bring innovative technologies and solutions to analytical science. SGE locations worldwide Technical Support This guide provides a comprehensive level of information. Should you require further support please don t hesitate to contact your local SGE office or techsupport@sge.com 2 For more information and support visit us at

3 Introduction to Biodiesel Analysis The rising price of oil is driving fuel prices to new highs causing substantial global economic impacts. This economic situation combined with the desire for cleaner and greener energy solutions has created the demand for new fuels. Companies and consumers are looking for alternative ways of deriving fuel from available sources. One alternative fuel achieving both the environmental and economic requirements is Biodiesel. Biodiesel is diesel derived from vegetable oils, animal fats and waste food by-products, usually cooking oil. Major investment is currently being made into the development of algae and other specifically bred plant crops that will increase yield and enable biodiesel to become a major component of our energy infrastructure. Product Process The most widely accepted form of biodiesel production is trans-esterification, where typically oil is mixed with a solvent, usually methanol, and catalyzed by sodum methoxide, which causes the oil to be split into biodiesel and glycerine by-products. The biodiesel will then be processed for use in vehicles and the waste products split-off and sold separately. Glycerols, the major waste components, need to be minimized to trace levels within the fuel to prevent engine malfunction. Both the ASTM International and European National (EN) standards for biodiesel fuel certification require the fuel to be tested for total glyercol content before it can be released for sale. The European biodiesel standard mandates the completion of additional tests such as methanol and FAMEs. Toolkit Contents Sample Preparation BPX-BIOD GC Capillary Columns Method Library: o ASTM 6584 / EN1415 Free Glycerine, Mono-glycerides, Di glycerides, Tri-glyerides o EN1413 Determination of ester and linolenic acid methyl ester contents o EN1411 Determination of methanol content Optimized chromatography supplies for Biodiesel Analysis Information Resources 3

4 Sample Preparation Biodiesel requires quantitative measurement of the composition of the sample. Consequently liquid handling accuracy and precision are critical to the success of the final application. evol, the world s first digitally controlled analytical syringe and 21 R&D1 award winner, provides the accuracy and precision required in Biodiesel analytical laboratories. evol XR makes Everyone an expert Speed up and simplify laboratory workflow Improve accuracy and reproducibility Standardize results independent of operator skill evol is the coupling of two precision devices: a digitally controlled electronic drive and an XCHANGE enabled analytical syringe. XCHANGE syringes are easily and quickly changed allowing them to be dedicated to individual liquids or methods to prevent possible cross-contamination of reagents. evol is ergonomic, comfortable and easy to use. evol is easily calibrated and calibration factors saved for each syringe, enabling laboratories to comply with stringent global laboratory standards evol is suitable for use with volatile samples. evol is programmable to store a laboratory workflow (up to 98 steps). evol is suitable for direct injection onto a chromatography column with a consistent flow rate. evol s stainless steel needle enables direct injection through septa. Now available! evol XR 1 ml syringe Password protection options enabling standardization of work processes Slow aspirate and dispense for titrations and reaction rate control. New 1 ml syringe provides more flexibility to improve workflows. Up to 2 syringes, methods and custom programs can now be saved. evol Electronic Starter Kit contains: Description evol XR evol s 5 μl, 1 μl and 1 ml - Stand - Universal Charger - Comprehensive Instruction Manual - Disc with Manual in Multiple Languages - evol only For more information and support visit us at

5 BPX-BIOD GC Capillary Columns SGE has evaluated each ASTM and EN biodiesel method to demonstrate the suitability of BPX BIOD GC columns to achieve optimal separation. Each BPX-BIOD GC column is tested at the maximum operating temperature for the column, and specific tests are undertaken to ensure the column meets inertness specifications and separation performance. This means you can be confident of a reliable separation, column after column. Please refer to the table below and method library on the following pages (6-14) to find the best high temperature column for your biodiesel analysis. Method Description Column ID Length (m) Film Thickness (µm) Part Number ASTM D6584* BPX BIOD SG53 ASTM D6584* BPX BIOD SG53 EN1413* BPX BIOD EN1415* BPX BIOD SG53 EN1411* BPX BIOD EN1411** BPX - BIOD Wax EN1411** BPX - BIOD Wax *Recommended column in method **Alternative column recommended in method Includes guard column 5

6 ASTM D6584 / EN 1415 Free and total Glycerine Column: Inlet Liner: Septum: BIOD-BPX6584 or BPX-BIOD5 1 M X.32 mm ID X.1 µm film w / 2 m.53 guard column. Part Number: 54118SG53 Baffle liner if using PTV injection HT Condition column at 39 C for 1. hours with 3 ml/min flow of helium Injection: PTV Inlet 6 C for.5 minute to 4 C at 14.5 C/ sec hold 22 minutes Splitless for 1. minutes (A PTV injection system was used as an equivalent technique to on- column injection ) GC Conditions: Oven: 5 C for 1 minute to 18 C at 15 C/min hold minutes to 23 C at 7 C/min hold minutes to 38 C hold 4.2 minutes (26.1) Flow: He 3./min constant flow, split 2:1 Detector (FID): 4 C Low Level ASTMD6584 / EN1415 Standard Glycerol Butanetriol (IS) Monopalmitin Monolein Tricaprin (IS) Diolein 2.13 Triolein High Level ASTMD6584 / EN1415 Standard Glycerol Butanetriol (IS) Monopalmitin Monolein Tricaprin (IS) Diolein Triolein For more information and support visit us at

7 Good yellow grease biodiesel and bad yellow grease biodiesel compared with ASTMD6584 / EN1415 Standard Glycerol Butanetriol (IS) Monopalmitin Monolein Tricaprin (IS) Diolein Triolein Good Yellow Grease Bad Yellow Grease Standard Soy derived biodiesel sample compared to ASTMD6584 / EN1415 Standard Glycerol Butanetriol (IS) Monopalmitin Monolein Tricaprin (IS) Diolein Triolein Soy derived biodiesel Standard

8 EN1413 Determination of ester and linolenic acid methyl ester contents Column: Inlet Liner: Septum: BPX-BIOD2 3M X.32mm ID X.25 um film: Part Number: FocusLiner Split HT Condition column at 26 C for 1.5 hours Ferrule: SilTite Ferrule Kit Autosampler 1 µl : Injection type: split 5:1 Injector Temp: 25 C Detector Temp: 28 C GC Conditions: Carrier Gas: Constant flow: Column Flow: He on 1.5 ml/min Oven: 21 C for 9 minutes to 24 C at 2 C/min hold 6 minutes (16.5 minutes) Injection volume: 1 µl via Autosampler 37 FAME Reference Standard , , , , RT # Component Wt% C14: (Myristic) C14:1 (Myristoleic) C15: (Pentadecanoic) C15:1 (cis-1-pentadecenoic) C16: (Palmitic) C16:1 (Palmitoleic) C17: (Heptadecanoic) C17:1 (cis-1-heptadecenoic) C18: (Stearic) C18:1n9c (Oleic) C18:1n9t (Elaidic) C18:2n6c (Linoleic) C18:2n6t (Linolelaidic) C18:3n6 (γ-linolenic) C18:3n3 (α-linolenic) 2 RT # Component Wt% C2: (Arachidic) C2:1n9 (cis-11-eicosenoic) C2:2 (cis-11,14-eicosadienoic) C2:3n6 (cis-8,11,14-eicosatrienoic) C21: (Henicosanoic) C2:3n3 (cis-11,14,17-eicosatrienoic) C2:4n6 (Arachidonic) C2:5n3 (cis-5,8,11,14,17-eicosapentaenoic) C22: (Behenic) C22:1n9 (Erucic) C22:2 (cis-13,16-docosadienoic) C23: (Tricosanoic) C24: (Lignoceric) C22:6n3 (cis-4,7,1,13,16,19-docosahexaenoic) C24:1n9 (Nervonic) 2 8 For more information and support visit us at

9 FAME profile of IQAEP B214 Yellow Grease C17: C18:1 24 C18: C18: C16: C14: C18:3 4 2 C2: C2:1 C22: C24: FAME profile of Good Yellow Grease C17: C18: SGE recommends HT septa and SilTite ferrules for leak free performance in high temperature applications C18: C18: 14 C16: C18:3 4 C14: 4 2 C2: C2:1 C22: C24:

10 EN1413 Continued FAME profile of Bad Yellow grease C17: C18: C18: C16: 12 C18: C14: 4 C18:3 2 C2: C2:1 C22: C24: FAME profile of Canola C18:1 24 C17: C18: C16: C18: C18: 4 2 C14: C2:1 C2: C22: C24: For more information and support visit us at

11 FAME profile of Sunflower C17: C18: C18: C16: 12 C18: C14: C18:3 2 C2: C2:1 C22: C24: FAME profile of Soy / Tallow C17: C18: SGE recommends HT septa and SilTite ferrules for leak free performance in high temperature applications C18: C16: 12 C18: C14: 4 C18:3 2 C2: C2:1 C22: C24:

12 EN1411 Methanol Content in Biodiesel Column: BPX-BIOD1 3 M X.32 mm ID X 3. µm film. Part Number: The analysis can also be accomplished on BPX-BIOD 3 M x.32 mm ID x.25 and 1. µm Film, conditions are available on request Inlet Liner: FocusLiner Split Septum: HT Ferrule: SilTite Ferrule Kit Inlet Temp: 15 C Detector Temp: 25 C GC Conditions: Carrier Gas: He Constant Flow: On Flow: Helium 1.5 ml/min, split 5:1 Helium 1. ml/min, split 5:1 Oven: 21 C for 9 minutes to 24 C at 2 C/min hold 6 minutes (16.5 minutes) Manual Injections Standard set up: Initial sample: 1 ml sample with 56.8 µl methanol (.5%) - Calibration Solution A 1st dilution: 2 ml initial sample + 8 ml BD (.1%) - Calibration Solution B 2nd dilution: 2 ml of 1st dilution + 9 ml of BD (.1%) - Calibration Solution C 5 gm of each Calibration Solution were placed in 2 ml headspace vials and 5 µl of isopropanol (IPA) was added to each vial and crimp sealed. The samples were placed in an 8 C oven 15 minutes apart for 45 minutes. The 25 µl syringe was placed in a 6 C oven during this period and between injections. Methanol content using EN1411 recommended column 2. ml/min flow - 5 C - 2 µl injection Methanol 2.3 min SGE recommends HT septa and SilTite ferrules for leak free performance in high temperature applications Propanol 3.7 min For more information and support visit us at

13 Calibration Solution A - 25 µl of.5% standard on BP1 column with 5 µl of IPA / 5 gms of sample Methanol 2.3 min Propanol 3.7 min Calibration Solution B - 25 µl of.1% standard on BP1 column with 5 µl of IPA / 5 gms of sample Methanol 2.3 min Propanol 3.7 min

14 EN1411 Continued Calibration Solution C - 25 µl of.1% standard on BP1 column with 5 µl of IPA / 5 gms of sample Propanol 3.7 min Methanol 2.3 min For more information and support visit us at

15 Optimized Chromatography Supplies for Biodiesel Analysis s 1-5 µl NanoVolume SPECIFICATIONS Accuracy and Reproducibility Borosilicate Glass Barrel Outer Diameter (OD) Scale Length ± 2 % (dispensed volume) 6.5 mm and 8 mm.5 µl (6.5 mm OD barrel) = 27.5 mm,.5 µl (8 mm OD barrel) = 63.7 mm, 1 µl = 54.1 mm, 63.7 mm, 5 µl = 48.7 mm International Standards Traceability Volume Length Gauge OD ID Tip Code Replacement and Plunger 8. mm Outer Diameter (OD) Barrel 1 µl Cone 1BR µl Bevel 1BR-5BV µl Cone 1BR µl Bevel 1BR-7BV µl Cone 1BR µl Cone 1BR-7/ µl Cone 5BR µl Bevel 5BR-5BV µl Cone 5BR µl Bevel 5BR-7BV µl Cone 5BR µl Nozzle 5BR-7N s Fitted with Repeating Adaptor The SGE Repeating Adaptor (RAX) is attached to a syringe to ensure reproducibiliaty of sample volumes. Volume Length Gauge OD ID Tip Code Replacement and Plunger 8. mm Outer Diameter (OD) Barrel 1 µl Cone 1BR-5-RAX µl Cone 1BR-7-RAX

16 Optimized Chromatography Supplies Manual and On Column s 1-5 µl PTFE Tipped Plunger SPECIFICATIONS Accuracy and Reproducibility Borosilicate Glass Barrel Outer Diameter (OD) Scale Length ± 1 % (dispensed volume) 1 µl = 6.5 mm, 25 5 µl = 8 mm 1 µl = 54.1 mm, 25 5 µl = 6 mm International Standards Traceability Volume PTFE Tipped Plunger Length Gauge OD ID Tip Code Replacement Replacement Plunger 6 Pack Part No. Part No. Fixed 1 µl Bevel 1F-GT µl Bevel 1F-GT µl Bevel 25F-GT µl Bevel 5F-GT µl Bevel 1F-GT µl Bevel 25F-GT µl Bevel 5F-GT Removable 1 µl Bevel 1R-GT µl Bevel 25R-GT µl Bevel 5R-GT µl Bevel 1R-GT µl Bevel 25R-GT µl Bevel 5R-GT Guided Plunger 1 µl Bevel 1R-GP-GT On-Column 1 µl On- Column 1R-GT-OC- CE For more information and support visit us at

17 GC Supplies for PerkinElmer Autosampler s All needles are 7 mm long with a cone point style. For PerkinElmer AutoSystem Volume Gauge (OD mm) Code Pack Size Spare Plunger Fixed 5 µl 26 (.47) 5F-PE-.47C µl 23 (.63) 5F-PE-.63C µl Gas Tight 26 (.47) 5F-PE-GT-.47C µl Gas Tight 23 (.63) 5F-PE-GT-.63C µl 23 (.63) 5F-PE-.63C Septa Pack Size Instrument Diameter Septum Type Pack Size PerkinElmer Sigma, 9, 99, 11 HT series, AutoSystem & Clarus 11 EC Inlet Liners Description and Geometry Sketch OD ID For AutoSystem & Clarus 5, 6 Length Split / Splitless FocusLiner Split, straight-through liner Pack Size SGE s new enhanced inlet liner range is colour coded by geometry to simplify selection: Ferrules Instrument Column ID Size of Nut Ferrule ID Pack Size 15% Graphite / 85% Vespel Ferrules mm 1/16".4 mm mm 1/8".4 mm mm 1/16".5 mm For Injectors & Detectors at.32 mm 1/8".5 mm atmospheric pressure e.g. FID mm 1/16".8 mm mm 1/8".8 mm /8" OD Packed Columns 1/8" 1/8" /4" OD Packed Columns 1/4" 1/4" SilTite Metal Ferrules mm.4 mm 1* 732 For GC-MS Interface Connection (Starter Kit).32 mm.5 mm 1* mm.8 mm 1* 7322 Replacement SilTite Ferrules mm.4 mm For GC-MS Interface Connection.32 mm.5 mm mm.8 mm /32".81 mm Replacement SilTite Nuts *Includes 1 ferrules, 2 SilTite nuts. 17

18 Optimized Chromatography Supplies GC Supplies for Agilent Technologies Autosampler s For Agilent 7673, 7683 & 685 ALS Instruments Volume Gauge (OD mm) Code Pack Size Spare Pack Size Spare Plunger Fixed Tapered 5 µl 23-26s 5F-AG-.63/.47C (.63/.47) 1 µl 23-26s 1F-AG-.63/.47C (.63/.47) 1 µl Gas Tight 23-26s 1F-AG-GT-.63/.47C (.63/.47) Fixed Straight 5 µl(m) 23 (.63) 5F-AG-.63C µl(m) 23 (.63) 1F-AG-.63C Removable Tapered 5 µl 23-26s 5R-AG-.63/.47C (.63/.47) 1 µl 23-26s (.63/.47) 1R-AG-.63/.47C Removable Straight 1 µl 23 (.63) 1BR-AG-.63C ** Pack Size Septa Instrument Diameter Septum Type Pack Size Agilent Technologies 789, 689, 589, 588, 489 & HT EC , 58 & , 71, 72, 81 & , 579, 588 & HT EC HT Pre-drilled EC HT Pre-drilled Inlet Liners Description and Geometry Sketch OD ID For Agilent 589, 685, 689, 789 and HP489 Length Split / Splitless FocusLiner Split, Straight-through Liner Pack Size SGE s new enhanced inlet liner range is colour coded by geometry to simplify selection: 18 For more information and support visit us at

19 Ferrules Instrument Column ID Ferrule ID Pack Size 15% Graphite / 85% Vespel Ferrules mm.4 mm mm.5 mm For Injectors & Detectors at atmospheric pressure e.g. FID.53 mm.8 mm for 1/8" OD Packed Columns 1/8" for 1/4" OD Packed Columns 1/4" mm.4 mm For GC-MS Interface Connection.32 mm.5 mm mm.8 mm SilTite Metal Ferrules mm.4 mm 1 *732 For GC-MS Interface Connection (Starter Kit).32 mm.5 mm 1 * mm.8 mm 1 * mm.4 mm 1 # 7327 For Split / Splitless Injectors (Starter Kit).32 mm.5 mm 1 # mm.8 mm 1 # /32".81 mm 1 # Replacement SilTite Metal Ferrules mm.4 mm For All Connections.32 mm.5 mm mm.8 mm /32".81 mm Replacement SilTite Nuts For GC-MS Interface Connection _ For Split / Splitless Injector _ Replacement SilTite Base Seals For Split / Splitless Injector _ _ * Includes 1 ferrules, 2 SilTite nuts. # Includes 1 ferrules, 2 SilTite nuts and 2 SilTite Inlet Base Seals. GC Supplies for Shimadzu Autosampler s All needles are 42 mm long with a cone point style. Shimadzu AOC14, AOC17 and AOC2 Volume Septa Gauge (OD mm) Code Instrument Septum Type Pack Size HT A, 14, 15A, 16, 17A, 21 & 214 EC Pack Size Spare Pack Size Spare Plunger Fixed 5 µl 26 (.47) 5F-S-.47C µl 23 (.63) 5F-S-.63C µl Gas Tight 23 (.63) 25F-S-GT-.63C Removable 1 µl 26 (.47) 1R-S-.47C µl 23 (.63) 1R-S-.63C µl Gas Tight 23 (.63) 1R-S-GT-.63C Pack Size 19

20 Optimized Chromatography Supplies GC Supplies for Shimadzu continued Inlet Liners Description and Geometry Sketch OD ID Length Pack Size For GC-21 (SPL-21 Injector), GC-214 (SPL-214 Injector) and GC-17A (SPL-17 Injector) Split / Splitless FocusLiner * Split / Splitless FocusLiner Split, straight-through liner * When using a standard 42 mm needle for autosamplers, the sample will be injected on top of the wool for this liner. O-rings and Sealing Rings Description Usage Pack Size Graphite Sealing Ring Can be used at temperatures up to 45 C. For 14, 15A & 16 (SPL-14 injector) Graphite Sealing Ring Can be used at temperatures up to 45 C. For 17A (SPL-17 injector) Viton O-Ring Can be used at temperatures up to 3 C. For 21 & 214 (SPL-21 Injector & SPL-214 Injector) SGE s new enhanced inlet liner range is colour coded by geometry to simplify selection: Ferrules Column ID Description Pack Size GC14A, GC17A, GC21 and GC214 Detector / Injectors (Not for MS interfaces or QP21 Injector) mm ID columns SilTite Metal - Initial Installation 1* mm ID columns SilTite Ferrules mm ID columns SilTite Metal - Initial Installation 1* mm ID columns SilTite Ferrules mm ID columns SilTite Metal - Initial Installation 1* mm ID columns SilTite Ferrules n/a SilTite Metal Nuts - Slotted QP5/55 Standard MS Interface QP5-I mm ID columns 15% Graphite/ 85% Vespel Ferrules QP5-I.32 mm ID columns 15% Graphite/ 85% Vespel Ferrules QP5-II & QP mm ID columns 15% Graphite/ 85% Vespel Ferrules QP5-II & QP55.32 mm ID columns 15% Graphite/ 85% Vespel Ferrules mm ID columns SilTite Metal - Initial Installation 1* mm ID columns SilTite Ferrules mm ID columns SilTite Metal - Initial Installation 1* mm ID columns SilTite Ferrules mm ID columns SilTite Metal - Initial Installation 1* mm ID columns SilTite Ferrules QP5/55 Wide Bore MS Interface, QP21 Injector and QP21 Standard MS Interface mm ID columns 15% Graphite/ 85% Vespel Ferrules mm ID columns 15% Graphite/ 85% Vespel Ferrules mm ID columns 15% Graphite/ 85% Vespel Ferrules mm ID columns SilTite Metal - Initial Installation 1* mm ID columns SilTite Ferrules mm ID columns SilTite Metal - Initial Installation 1* mm ID columns SilTite Ferrules mm ID columns SilTite Metal - Initial Installation 1* mm ID columns SilTite Ferrules n/a SilTite Metal Nuts * Includes 1 ferrules, 2 SilTite nuts. 2 For more information and support visit us at

21 Replacement Parts Material Pack Size Replacement SilTite Nuts For 21 GC-MS System For 21 GC-MS System with QP5 series MS For 21/214 GC Injectors and atmospheric detectors QP5 Jet Separator MS Interface QP5 Direct MS Interface For All Injectors Jet Separator (Starter Kit), except 21/ GC Supplies for Thermo Scientific Autosampler s All needles have a cone point style. For Thermo Scientific / CE Instruments / Fisons Autosampler Specifications TriPlus AS3 AS2 AS2/8 Volume Length Gauge Code Pack Size Fixed 5 µl 5 26 (.47) 5FX-5C µl 8 23 (.63) 1F-C/T-8/.63C µl 8 26 (.47) 1F-C/T-8/.47C µl 5 25 (.5) 1F-C/T-5/.5C 195 Removable 1 µl 5 23 (.63) 1R-C/T-5/.63C µl 8 26 (.47) 1R-C/T-8/.47C Septa Instrument Diameter Septum Type Pack Size 8 Series, FOCUS, 17 HT Trace/ULTRA GC 17 EC Inlet Liners Description and Geometry Sketch OD ID For TRACE and Focus Split FocusLiner (for use with 5 mm ) Split, Straight-through Liner Length Pack Size Part no Sealing Rings Description Usage Pack Size Graphite Sealing Ring 8 mm ID. For 8 & TRACE GCs

22 Optimized Chromatography Supplies GC Supplies for Thermo Scientific continued Ferrules Instrument Column ID Ferrule ID Pack Size 15% Graphite / 85% Vespel Ferrules mm.4 mm mm.5 mm For All Injectors & Detectors (Not for 8 Series, Focus, Trace/Ultra GC).53 mm.8 mm /8" OD Packed Columns 1/8" /4" OD Packed Columns 1/4" mm.4 mm 1# For All Focus, Trace/Ultra Injectors & Detectors at atmospheric pressure e.g. FID (Not for GC-MS).32 mm.5 mm 1# mm.8 mm 1# Brass Nut for Focus, Trace/Ultra GC Injectors & Non-MS Detectors SilTite Metal Ferrules mm.4 mm 1* 7345 For GC-MS Interface Connections (Starter Kit).32 mm.5 mm 1* mm.8 mm 1* /32".81 mm 1* Replacement SilTite Metal Ferrules mm.4 mm For All GC-MS Interface Connections.32 mm.5 mm mm.8 mm /32".81 mm Replacement SilTite Nuts # To be used in combination with brass nut ( 13485) * Includes 1 ferrules, 2 SilTite nuts. GC Supplies for Varian / Bruker Autosampler s Volume Length Gauge (OD mm) Tip Style Code Pack Size Part No. Pack Size Plunger Varian/Bruker 835, 81 and 82 Fixed 1 µl Gas Tight (.5) S/Hole 1F-GT-VA8X-II Removable 1 µl* (.47) Cone 1BR-VA8X ** 1 µl Gas Tight (.5) S/Hole 1R-GT-VA8X-II Alternatives for P/N (.5) Bevel N1-VA835-II OC N1-VA II (.63) S/Hole N1-VA8XH--.63-II (.5) S/Hole N1-VA8H-II(.2) Varian/Bruker CP-84/841, CP-91/95 Fixed 1 µl 5 26 (.47) Bevel 1F-VA84-5/ µl 5 23 (.63) Cone 1F-C/T-5/.63C Removable 1 µl 5 26 (.47) Cone 1R-C/T-5/.47C *Not suitable for 82 autosampler. **Denotes Plunger and Kit. Pack Size 22 For more information and support visit us at

23 Septa Instrument Diameter Septum Type Pack Size Varian/Bruker 1177 Injector 14, 141, 16 & 161 Injectors 9 HT EC HT EC Inlet Liners Description and Geometry Sketch OD ID For 1177 Injector Length Split / Splitless FocusLiner Split, straight-through liner Split FocusLiner Straight-through Liner O-rings and Sealing Rings Pack Size Part no SGE s new enhanced inlet liner range is colour coded by geometry to simplify selection: Description Usage Pack Size Viton O-Ring for 1177 Injector Can be used at temperatures up to 3 C Graphite Sealing Ring for 175 & 177 Injectors Graphite Sealing Ring for 178 & 179 Injectors Ferrules Instrument Column ID Ferrule ID Pack Size 15% Graphite / 85% Vespel Ferrules For GC-MS & Detectors at atmospheric pressure e.g. FID SilTite Metal Ferrules For GC-MS Interface Connections (Starter Kit) Replacement SilTite Ferrules For GC-MS Interface Connections Replacement SilTite Nuts * Includes 1 ferrules, 2 SilTite nuts mm.4 mm mm.5 mm mm.8 mm mm.4 mm 1* mm.5 mm 1* mm.8 mm 1* /32".81 mm 1* mm.4 mm mm.5 mm mm.8 mm /32".81 mm

24 Information Resources For Official Methodolgy ASTM International ASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. DIN Deutsches Institut fur Normung A range of international standards and reference for EN methods. Industry resources National Biodiesel Board Body responsibly for the development of the US biodiesel industry. Journals BioDiesel Magazine Biodiesel industry journal for news on commercial and technical developments BioFuels Magazine Industry journal for biofuels that features biodiesel related news. Distributor Stamp BR-392-G_RevE SGE Analytical Science Pty Ltd 4/212 AUSTRALIA & PACIFIC REGION SGE Analytical Science Pty Ltd Toll Free: Tel: +61 () Fax: +61 () support@sge.com JAPAN SGE Japan Inc Tel: Fax: japan@sge.com EUROPE SGE Europe Ltd European Head Office Toll Free: Toll Free Fax: Tel: Fax: Tel France: Fax France: Tel Germany: +49 () 6155 / 6746 Fax Germany: +49 () 6155 / europe@sge.com UNITED STATES OF AMERICA SGE Incorporated Toll Free: (8) Tel: Fax: usa@sge.com