2011 Liquid Handling Product Catalog

Transcription

1 2011 Liquid Handling Product Catalog

2 The hummingbird The sensitive and precise qualities of the hummingbird symbolize Biohit s product groups of liquid handling, diagnostic products, and analysis systems. Biohit s liquid handling products based on the company s innovations and technologies are characterised by accuracy, precision and quality, as well as ergonomics and safety. 2 Biohit Liquid Handling Products

3 Catalog Contents Pipettes 5 Products & Innovations 6 Choosing the Right Pipette 7 Mechanical Pipettes mline Single & Multichannel Pipettes 8 Proline Plus Single & Multichannel Pipettes 14 Proline Plus Single-Channel Fixed Volume Pipettes 17 Proline Single & Multichannel Pipettes 20 Proline Single-Channel Fixed Volume Pipettes 23 Electronic Pipettes eline Single & Multichannel Pipettes 24 Proline Single & Multichannel Pipettes 28 epet Single & Multichannel Pipettes 32 Pipetting Modes 35 Safe-Cone Filters 36 Optifit Pipette Tips 37 Non-Filtered Tips & SafetySpace Filter Tips 40 Packaging Options 41 Tip Specifications 42 Pipette - Tip Compatibility Charts 44 Premium Tip Quality 46 Dispensers & Tips 47 eline Lite & Pro Dispenser 48 Mechanical Stepper 51 Dispenser Tips 52 Accessories and Instruments 53 Ergotube 54 Ergomate 55 Reagent Vessel 56 Readers and Washers 57 Pipette Stands 58 Maxi-Volume Liquid Handling 59 Midi Plus 60 Midi Pro 62 Prospenser Bottle Top Dispenser 63 Biotrate Digital Burette 64 Biohit Liquid Handling Products 3

4 Custom-Made Solutions 65 OEM Solutions 67 rline Robotic Dispensing Module 68 Pipetting Academy 69 Pipetting Academy - the Concept 70 Ergonomics 74 Safety in Pipetting 75 Accuracy & Precision 76 Pipetting Preparations, Pipetting Action & Other Cautions 77 Pipette Calibration & Maintenance 79 Pipette MD and Biohit Service Providers 81 Pipette MD Calibration Services 82 Pipette MD Diagnostic and Repair Services 84 Pipette MD Preventative Maintenance Services 85 Pipette Decontamination Procedure 87 Autoclaving Instructions, Troubleshooting Guide 88 About Biohit 89 Quality Standards 90 Literature 91 Brochures 92 4 Biohit Liquid Handling Products

5 Enjoy your work! Pipettes Biohit Liquid Handling Products 5

6 We offer more than just pipettes Founded in Finland in 1988, Biohit has established its position in the world market with its innovative, high technology liquid handling products such as pipettes and pipette tips. The majority of the electronic pipettes used in laboratories worldwide are developed by Biohit. Combining performance, ergonomics and experience we are able to provide safe, accurate and reproducible pipetting in the most demanding liquid handling applications. Outstanding quality We can guarantee the accuracy and precision of our pipettes (see page 78), and help you optimize your results, when you use them together with our Optifit tips (see pages 37-46). Maximum comfort Ever since we developed the first Proline pipette, we have aimed at improving ergonomics of pipette design and usability. Because we wish to reduce the risk of upper limb disorders, such as RSI, and let you enjoy your work. (see pages 71-76) Enhanced safety Safety is not only about ergonomics and high-quality tools but also about protecting the tools and the sample from contamination in order to obtain accurate and reliable results. (see pages 77-80) Think economical, think ecological Cost-efficient product features, service contracts and low lifetime costs of our products help you save money and get a high return on investment. We also aim to develop and manufacture products that will cause as little environmental loading as possible throughout their entire life cycles. Extended life-time We extend the life time of our products through our professional maintenance and calibration services that are provided by our certified service centers worldwide (see pages 81-90). Sharing our knowledge and experience with you After more than 20 years in liquid handling, we firmly believe we have gained quite a lot of experience and knowledge in the field. Pipetting Academy is the tool for us to help you learn ergonomics, proper tool selections and how to get the most accurate results. (see pages 71-75). Enjoy your work! Special pricing available for GSA contract customers - contact your local VWR representative for more information Innovating for Health since Biohit Liquid Handling Products

7 Choosing the right pipette - selection guides Pipettes Mechanical Pipettes (page 8) (page 14) (page 20) Electronic Pipettes (page 24) (page 28) (page 32) Functions mline Proline Plus Fully autoclavable Optiload all models multichannels only Proline Safe-Cone filters Filter ejector Thermal insulation Volume locking click stops click stops Pipetting force 1 12 N 15 N 20 N Weight 1 77 g 82 g 84 g Color coding on display Color coding caps 2 ID tags Biohit 6 place Carousel Stand Proline 5 place carousel stand Biohit Linear Stand Pipette holder 2 Warranty 3 years 3 years 3 years CE/IVD marked Starter Kits available eline Lite & Pro Dispenser Operating Modes Operating modes eline Pro eline Lite Multiple Dispensing with the Tip Guide (d) Multiple Dispensing (d) Pipetting (P) Diluting (dd) Sequential Dispensing (Sd) Automatic Multiple Dispensing (Ad) Multi-Aspirating (SA) Custom mode (CST) - GL (select service intervals) - Sr (select the desired tip range) - SET (returns default settings) 5 speeds for aspirating and dispensing Functions eline Proline epet Pipetting (P) Multiple Dispensing (d) Diluting (dd) 1 Reverse Pipetting (rp) 2 Sequential Dispensing (Sd) 3 Manual Pipetting (Pm) 4 Automated Dispensing (Ad) Multi Aspirating (SA) 5 Memory places for favourite programs Post Delivery Mixing in P and dd Speed Selection 9 speeds 5 speeds 5 speeds Manual Tip Ejection Electronic Tip Ejection Autoclavable lower part 6 Low battery indicator Single point calibration Optiload Notes: 1 (dd) Available on all models except the 1200 µl multichannel models, 2 (rp) Available on all models except the 1200 and 5000 µl models, 3 (Sd) Only on the 1200 and 5000 µl models, 4 (Pm) Available in 1-ch models only, 5 (SA) Only on the 1200 µl models. 6 Except multichannel 1200 µl models µl / 1- channel model 2 Available as accessories multichannels only Direct charging with ACadaptor Optional Included Charging Stand (Holds 1 pipette) Charging Carousel (Holds 4 pipettes) Linear Stand Warranty 2 years 2 years 2 years CE/IVD marked Biohit Liquid Handling Products 7

8 Effortless Tip Loading Ejection & Fully autoclavable IVD Effortless accuracy meet the mline family! 8 Biohit Liquid Handling Products Biohit s most advanced mechanical pipette family the mline offers a unique selection of innovative & patented features for manual pipetting. Excellent performance, smooth ergonomics and perfect safety makes the mline. It is comfortable to use, hour after hour, day after day without the risk of strains (RSI). Enjoy your work. With mline pipettes you can. mline pipettes cover the full volume range of 0.1 µl to 10 ml.

9 Pipettes mline - Recommended by health and safety officers around the globe Prevents RSI and improves pipetting results through the exceptionally light pipetting forces and ergonomic design Perfect tip sealing with Optiload tip loading mechanism Contamination free pipetting with Safe-Cone Filters Easiest pipette to maintain only three parts to disassemble Highly durable, long-lasting materials highly chemically and UV resistant robust mechanics through innovative spring design Recommended by Health & Safety Officers 5 color coding caps Thumb activated filter ejection (page 9) Thermal insulation of the internal components to improve accuracy Clear and easy-to-read display with big numbers Locking mechanism preventing accidential volume changes during pipetting Fully autoclavable Fast and light, stepless volume setting with small increments Volume range color coding for easy selection of the right Biohit tip Easy cleaning and maintenance without any opening tool Ergonomic finger support, no need to squeeze the pipette What s the difference? Tips on pages Effortless Tip Loading Ejection & Improved tip fitting e Filters on page 36 Stand options available on page 58 Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 Autoclaving instructions on page 88 All models > 10 µl offer replaceable Safe-Cone Filters and unique filter ejection to prevent internal contamination and damage 0.3/1.2 KG 0.5/1.5 KG 0.9/2.0 KG mline Proline Plus Proline Comparison of the pipetting/blow-out forces needed. See page 7 for the selection guide. mline Mechanical Single-Channel Performance specifications and ordering details Cat. No. Channels Volume Range (μl) Increment (μl) µl µl µl µl µl µl µl ml 20.0 Test Volume (μl) Inacc. (%) 1.30 % 2.40 % % 2.50 % 5.00 % 0.90 % 1.20 % 3.00 % 0.80 % 2.00 % 0.60 % 0.80 % 2.00 % 0.60 % 0.60 % 0.50 % 0.60 % 2.00 % 0.60 % 1.20 % 3.00 % Impr. (%) 0.80 % 1.60 % 6.00 % 0.60 % 4.00 % 0.40 % 2.00 % 0.15 % 0.40 % 0.15 % 0.30 % 0.80 % 0.20 % 0.20 % 0.40 % 0.20 % 0.30 % 0.60 % 0.20 % 0.30 % 0.60 % Safe-Cone Filters Standard Plus Compatible Tip ID - - A, B, L, M - - A, B, L, M C, N C, D, O C, D, P, Q E, G, S J U The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. FINAS Accredited Calibration Certificate (ISO 17025) available for new pipettes **Biohit reserves the right to modify or substitute the free reservoir offer, or discontinue without notice. FREE! Reservoir offer: In every mline single-channel and multichannel box, a free single-chamber reservoir will be included free of charge** For additional info on the Biohit reservoirs, please see page 56. Pipetting Academy on page 70 Biohit Liquid Handling Products 9

10 Color coding caps Thumb activated filter ejection Light plunger button action Ergonomic finger support and ID tag for personal identification NEW! Ergomate on page 55 Clear and easy-to-read display with big numbers and small increments Rotating dispensing head for optimal pipetting convenience Locking mechanism preventing accidental volume changes during pipetting All models > 10 µl offer replaceable Safe-Cone Filters and unique filter ejection to prevent internal contamination and damage Individual piston / tip cone assemblies for easy maintenance Effortless Tip Loading Ejection & Fully autoclavable Optiload tip loading mechanism for perfect tip sealing and light tip ejection e Tips on pages Filters on page 36 Stand options available on page 58 Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 Autoclaving instructions on page 88 mline Mechanical Multichannel Performance specifications and ordering details FREE! Reservoir offer: In every mline single-channel and multichannel box, a free single-chamber reservoir will be included free of charge** For additional info on the Biohit reservoirs, please see page 56. Cat. No. Channels Volume Range (μl) Increment (μl) channel µl channel µl channel µl channel µl 0.01 Test Volume (μl) Inacc. (%) 2.50 % 4.00 % 0.70 % 3.00 % 0.60 % 2.00 % 2.50 % 4.00 % Impr. (%) 2.50 % 4.00 % 0.25 % 0.70 % 0.25 % 0.50 % 2.50 % 4.00 % Safe-Cone Filters Standard Plus Tip ID - - A, B, L, M C, D, O D, Q - - A, B, L, M channel µl % 3.00 % 0.25 % 0.70 % C, D, O channel µl % 2.00 % 0.25 % 0.50 % D, Q The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. **Biohit reserves the right to modify or substitute the free reservoir offer, or discontinue without notice. 10 Biohit Liquid Handling Products

11 Pipettes No rocking needed Effortless Tip Loading Ejection & Unique protection with Safe-Cone Filters To prevent contamination of the pipette and the sample, the mline pipettes feature replaceable Safe-Cone Filters. The unique Safe-Cone Filter ejection of mline means that the filters can be easily and safely removed by pressing the plunger button. Safe- Cone Filters are available for both single- and multichannel models > 10 µl. Filters on page 36 Optiload tip loading and perfect tip sealing All mline pipettes are equipped with spring loaded tip cones the Optiload mechanism developed by Biohit, that Allows tips to be loaded with constant force Enables even and safe tip sealing to the tip cone(s) with minimum force Makes tip ejection light and easy Is an advantage in multichannel models, in particular, where even tip sealing is essential. Unique spring mechanism for lighter pipetting The mline pipette has a patented spring mechanism (plunger technology) resulting in the exceptionally light pipetting forces. This together with light tip ejection force and ergonomic design of the handle reduces the risk of Repetitive Strain Injury (RSI). The qualities that really convinced me to switch to Biohit mline are its light weight, lowest pipetting and tip ejection forces, easy to read display, locking mechanism to prevent accidental volume changes and finally, its compatibility to tips currently used at our company. Richard P. Roque, Preclinical Development, a Seattle based biotechnology company, USA Conventional pipettes use compression spring mechanism resulting in heavy pipetting force. The tension spring mechanism used in Biohit mline pipettes makes pipetting force almost 4 times lighter. Moreover, thanks to the unique mechanism the starting force is always constant regardless of the volume. UNIQUELY BIOHIT! N Biohit mline Competitor Pipette A Force to the 1st stop Competitor Pipette B Competitor Pipette C Competitor Pipette D Force to the 2nd stop Competitor Pipette E Pipetting force comparison of mline (1000 µl model) and other major pipette brands. The mline provides significantly lighter pipetting force than other mechanical pipettes. Measurements have been carried out in Biohit R&D development by using force measurement equipment Mecmesin Imperial 2500 Conventional pipettes N Compression spring mechanism Biohit mline 12 N Tension spring mechanism e Ergonomics on page 70 Biohit Liquid Handling Products 11

12 Easy maintenance and calibration The mline can be easily calibrated using the elegant calibration tool. The tool also acts as a tube cap opener. The mline has been designed for easy cleaning and maintenance without any opening tool. Only 3 parts need to be cleaned. The mline should, like any other pipette, be stored in an upright position. There are several different stand options for mline: Handy holder that is included with each mline pipette Carousel stand for six pipettes Stylish linear stand that allows the pipette to be placed on both sides of the stand Stands & Accessories Cat. No. Item Carousel Stand for 6 pipettes Linear Stand 12 Biohit Liquid Handling Products

13 Pipettes Get started with mline Starter Kit! Starter Kit Biohit mline Starter Kits offer an opportunity to test and get started with mline pipettes. You can choose between Starter Kits with a set of three or four single-channel mline pipettes. Biohit mline Starter Kit 1 - Cat. No mline Starter Kit. Includes: 1 single channel in each of the following volume ranges: 2-20ul, ul, and ul. A selection of Biohit tips included. Biohit mline Starter Kit 2 - Cat. No mline Starter Kit. Includes: 1 single channel in each of the following volume ranges: , 2-20ul, ul, and ul. A selection of Biohit tips included. Biohit Liquid Handling Products 13

14 Proline Plus Effortless Tip Loading Ejection & Fully autoclavable IVD Durable and dependable Proline Plus In the new Proline Plus pipette, Biohit has combined the basic functionalities of the traditional Proline mechanical with new, state-of-the-art pipette design and technology. Particular emphasis has been put on safety, ergonomics, and efficiency in pipetting, as well as on the high quality of the device. Proline Plus pipettes cover the full volume range of 0.1μl-10ml, in both adjustable and fixed volume models. 14 Biohit Liquid Handling Products

15 Pipettes Plus Is More! Proline Plus the classic Biohit Proline reborn Robust design Good UV resistance Excellent chemical resistance Small increments Reduced pipetting and tip ejection force Fully autoclavable Thermal insulation of the internal components ensures that the warmth of the hand does not affect the pipetting results during long pipetting series Robust design Good chemical & UV resistance Volume setting with click stops Suitable for both left and right-handed users Color coding on display corresponds to the color coding of tip trays Fully autoclavable Fits the most commonly used lab tubes thanks to the slim tip ejector collar What s the difference? Tips on pages e Filters on page 36 Stand options available on page 58 Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 Autoclaving instructions on page 88 Proline Plus Mechanical Single channel Performance Specifications and Ordering Details Cat. No. Channels Volume range (μl) Increment (μl) µl µl µl µl µl µl µl µl ml 20.0 Test volume (μl) Inacc. (%) 1.30 % 2.40 % % 2.50 % 5.00 % 0.90 % 1.20 % 3.00 % 0.90 % 1.40 % 3.00 % 0.80 % 2.00 % 0.60 % 0.80 % 2.00 % 0.60 % 0.60 % 0.50 % 0.60 % 2.00 % 0.60 % 1.20 % 3.00 % Impr. (%) 0.80 % 1.60 % 6.00 % 0.60 % 4.00 % 0.40 % 2.00 % 0.30 % 0.80 % 1.60 % 0.15 % 0.40 % 0.15 % 0.30 % 0.80 % 0.20 % 0.20 % 0.40 % 0.20 % 0.30 % 0.60 % 0.20 % 0.30 % 0.60 % Safe-Cone Filters to prevent contamination Safe-Cone Filters Standard Plus /1.2 KG Compatible Tip ID A, B, L, M A, B, L, M C, N C, Q C, D, O C, D, P, Q E, G, S J U 0.5/1.5 KG 0.9/2.0 KG mline Proline Plus Proline Comparison of the pipetting/blow-out forces needed. See page 7 for selection guide. Pipetting Academy on page 70 The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. FINAS Accredited Calibration Certificate (ISO 17025) available for new pipettes Biohit Liquid Handling Products 15

16 Volume setting with click stops Suitable for both left and right-handed users Optiload tip loading and perfect tip sealing All Proline Plus multichannel pipettes are equipped with unique spring loaded tip cones the Optiload mechanism developed by Biohit, that: Allows tips to be loaded with constant force Enables even and safe tip sealing to the tip cones with minimum force Makes tip ejection light and easy Is an advantage in multichannel models, where even tip sealing is essential Good chemical & UV resistance Safe-Cone Filters to prevent contamination Robust design Fully autoclavable Color coding on display corresponds to the color coding of tip trays Thermal insulation of the internal components ensures that the warmth of the hand does not affect the pipetting results during long pipetting series Unique Optiload tip loading for easy tip loading and ejection, as well as excellent tip sealing in multichannel models Effortless Tip Loading Ejection & NEW! Ergomate on page and 12-channel pipettes available Proline Plus Mechanical Multichannel Performance specifications and ordering details Cat. No. Channels Volume range (μl) Increment (μl) Test volume (μl) Inacc. (%) Impr (%) Safe-cone filters Standard Plus Compatible Tip ID µl % 4.00 % 2.50 % 4.00 % - - A, B, L, M µl % 3.00 % 0.25 % 0.70 % C, D, O µl % 2.00 % 0.25 % 0.50 % D, Q µl % 4.00 % 2.50 % 4.00 % - - A, B, L, M µl % 3.00 % 0.25 % 0.70 % C, D, O µl % 2.00 % 0.25 % 0.50 % D, Q 16 Biohit Liquid Handling Products The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice.

17 Pipettes Color coding on display corresponds to the color coding of tip trays A wide range of fixed volume models available The slim tip cone design also fits narrow laboratory tubes. Tips on pages Filters on page 36 Stand options available on page 58 e Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 Autoclaving instructions on page 88 Fully autoclavable Proline Plus Single-Channel FIXED Volume Performance specifications and ordering details Cat. No. Channels Volume range (μl) Increment (μl) Test vol. (μl) Inacc. (%) Impr. (%) Safe-cone filters Standard Plus Compatible Tip ID µl % 1.20 % - - A, B, L, M µl % 0.80 % - - A, B, L, M µl % 0.50 % C, N µl % 0.30 % C, O µl % 0.30 % C, O µl % 0.30 % C, D, O µl % 0.20 % C, D, P, Q µl % 0.20 % E, G, R, S µl % 0.20 % E, G, R, S µl % 0.20 % E, G, S µl % 0.15 % J µl % 0.15 % J ml % 0.20 % U Pipetting Academy on page 70 The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. Biohit Liquid Handling Products 17

18 The Proline Plus pipette should be stored in an upright position. There are several different stand options: Carousel stand for six pipettes Stylish linear stand that allows the pipette to be placed on both sides of the stand A handy holder that is available as an accessory Stands & Accessories Cat. No. Item Biohit Carousel Stand for 6 pipettes Linear Stand for all Biohit pipettes Prevent contamination with Safe-Cone Filters Proline Plus features unique Safe-Cone Filters that: Prevent fluids and liquid vapors from reaching the internal components of the pipette Help you prevent contamination of your pipette and sample in your everyday work Prolong the pipette life-time Are replaceable Can be removed by using convenient forceps Are available for all models > 10 μl Basic calibration and maintenance made easy No opening tools are needed Only 3 parts need to be cleaned piston, tip cone and tip ejector collar Calibration is easy with the tool included in the package If you wish to leave it to a professional, Biohit offers complete maintenance, repair and calibration services 18 Biohit Liquid Handling Products

19 Pipettes Get started with Proline Plus Starter Kit! Proline Plus Starter Kit Biohit Proline Plus Starter Kits offer an opportunity to test and get started with Proline Plus pipettes. You can choose between Starter Kits with a set of three or four single-channel Proline Plus pipettes. Biohit Proline Plus Starter Kit 1 - Cat. No ul, ul & μl Single Channel Proline Plus models. A selection of Biohit Pipet Tips included. Biohit Proline Plus Starter Kit 2 - Cat. No ul, 2-20ul, ul & μl Single Channel Proline Plus models. A selection of Biohit Pipet Tips included. Biohit Liquid Handling Products 19

20 Proline IVD The original Biohit mechanical pipette Proline Almost 20 years in the top of the world of pipettes, designed and manufactured by Biohit. In that time Proline pipettes have become a synonym for high quality. Working with Proline, you will get many years of reliable service. The Proline pipettes cover the volume range 0.1 μl to 5000 μl with 8 different adjustable single-channel and 8 adjustable multichannel models. Proline is also available in fixed volume single-channel models. 20 Biohit Liquid Handling Products

21 Pipettes Finger support keeps the instrument in place with minimum user effort Volume setting by click stops Tip ejector allows convenient one-handed operation Digital display clearly reads volume setting Proline - a good basic pipette Designed for many different types of routine laboratory work Lightweight design Good accuracy and precision Volume setting by click stops Full documentation, warranties and certificates Safe-Cone Filters to prevent internal contamination and damage Durable tip cone materials provide excellent chemical resistance Ejector collar and tip cone can be removed for easy cleaning and maintenance What s the difference? Tips on pages /1.2 KG 0.5/1.5 KG 0.9/2.0 KG e Filters on page 36 Stand options available on page 58 Ergomate on page 55 Ergonomics on page 70 All models > 10 µl offer replaceable Safe-Cone Filters to prevent internal contamination and damage mline Proline Plus Proline Comparison of the pipetting/blow-out forces needed. See page 7 for selection guide. Pipette selection guide on page 7 Proline Mechanical Single-Channel Performance specifications and ordering details Cat. No. Channels Volume Range (μl) Increment (μl) Test Volume (μl) Inacc. (%) Impr. (%) Safe-Cone Filters Standard Plus Tip ID µl % 3.00 % % 2.00 % 3.00 % 6.00 % - - A, B, L, M µl % 0.80 % - - A, B, C, L, M, N µl % 1.20 % 3.00 % 0.40 % 2.00 % C, N, O µl % 0.90 % 2.00 % 0.30 % 0.60 % 2.00 % C, D, O µl % 3.00 % 0.20 % 0.40 % C, D, O, P, Q µl % 0.80 % 2.50 % 0.20 % 0.30 % 0.80 % C, D, P, Q µl % 0.70 % 2.00 % 0.20 % 0.25 % 0.70 % E, G, S µl % 0.60 % 0.70 % 0.20 % 0.30 % 0.30 % J The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. FINAS Accredited Calibration Certificate (ISO 17025) available for new pipettes Biohit Liquid Handling Products 21

22 Simply turn the plunger button for volume selection Volume setting by click stops Tip ejector works with dispensing head in any position 8- and 12-channel pipettes are available Convenient pipette holder included with each pipette Dispensing head rotates for optimum pipetting convenience Individual piston/tip cone assemblies allow easy repair and maintenance All models > 10 µl offer replaceable Safe-Cone Filters to prevent internal contamination and damage Sequential tip ejection allows easy one-hand tip ejection Tips on pages Filters on page 36 Stand options available on page 58 Tip cone design allows easy tip loading from trays and visual seal verification e Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 NEW! Ergomate on page 55 Proline Mechanical Multichannel Performance specifications and ordering details Cat. No. Channels Volume Range (μl) Increment (μl) Test Volume (μl) Inacc. (%) Impr. (%) Safe-Cone Filters Standard Plus Tip ID channel µl % 4.00 % 2.50 % 4.00 % - - A, B, L, M channel 5-50 µl % 0.50 % 2.00 % C, D, O, P, Q channel µl % 0.25 % 0.50 % 0.80 % D, Q channel µl % 4.00 % 2.50 % 4.00 % - - A, B, L, M channel 5-50 µl % 0.50 % 2.00 % C, D, O, P, Q channel µl % 0.25 % 0.50 % 0.80 % D, Q The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. 22 Biohit Liquid Handling Products

23 Pipettes See Proline features on pages Fixed volume pipette has white display to differentiate it from adjustable volume Proline pipettes Proline carousel stand for 5 pipettes Stands & Accessories Cat. No. Item e Tips on pages Filters on page 36 Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page Proline Carousel Stand for 5 mechanical pipettes Linear Stand Proline Single-Channel FIXED Volume Performance specifications and ordering details Cat. No. Channels Volume Range (μl) Increment (μl) Test Volume (μl) Inacc. (%) Impr. (%) Safe-Cone Filters Standard Plus Tip ID channel 5 µl % 1.20 % - - A, B, C, L, M, N channel 10 µl % 0.80 % - - A, B, C, L, M, N channel 20 µl % 0.50 % C, D, N, O channel 25 µl % 0.30 % C, D, O channel 50 µl % 0.30 % C, D, O channel 100 µl % 0.30 % C, D, O, P, Q channel 200 µl % 0.20 % C, D, P, Q channel 250 µl % 0.20 % E, G, R, S channel 500 µl % 0.20 % E, G, R, S channel 1000 µl % 0.20 % E, G, S channel 2000 µl % 0.15 % J channel 5000 µl % 0.15 % J The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. FINAS Accredited Calibration Certificate (ISO 17025) available for new pipettes. Biohit Liquid Handling Products 23

24 Autoclavable lower parts IVD Leader in performance & ergonomics eline When you need to pipette and dispense liquids as comfortably as possible, your choice should be eline. Twice as fast as a mechanical pipette, this honorary mentioned (Pro Finnish Design 2001) electronic pipette takes analytical performance and ergonomic innovation to totally new levels! Its fully electronic operation including tip ejection gives you the chance to concentrate the work-effort to where it s needed the most. eline pipettes cover the full volume range of 0.2 to 5000 μl. 24 Biohit Liquid Handling Products

25 Pipettes eline - leader in performance and ergonomics Excellent accuracy and precision with the unique DC motor concept Fully electronic operations guarantee user-independent results Makes pipetting faster and easier Built-in error control for more reliable results Easiest tip ejection ever with the unique electronic tip ejection Ergonomic design that suits both leftand right-handed You need only one pipette instead of two one eline covers the volume ranges of two mechanical pipettes Comprehensive range of liquid handling protocols with easy programming (see page 27) Attractive ergonomic design Warning when low battery Easy-to-use keypad enables fast programming Unique electronic one-touch tip ejection Single-point calibration for specific liquids Direct charging option with AC-adaptor with immediate pipetting possibility Clear LCD-display with all the information you need Electronic start button for all pipetting operations Both left- and righthanded operation Volume range color coding for easy pipette and tip selection Ejector collar, tip cone and piston can be unscrewed for easy cleaning, autoclaving and maintenance Recommended by Health & Safety Officers Autoclavable lower parts e Tips on pages Filters on page 36 Stand options available on page 58 Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 Autoclaving instructions on page 88 WATCH VIDEO ON Replaceable Safe-Cone Filters help prevent contamination of the pipette and samples eline Electronic Single-Channel Performance specifications and ordering details Biohit recommends yearly preventative maintenance on all electronic pipettes. See page 82 for more information Cat. No. Channels Volume Range (μl) Increment (μl) Test Volume (μl) Inacc. (%) Impr. (%) Safe-Cone Filters Standard Plus Tip ID FREE! channel µl 1-channel µl 1-channel µl 1-channel µl % 2.50 % 0.40 % 0.60 % 2.00 % 0.40 % 0.60 % 0.40 % 0.60 % 0.40 % 0.70 % 0.15 % 0.20 % 0.15 % 0.20 % 0.80 % 0.15 % 0.20 % 0.50 % - - A, L, M C, D, O D, Q E, G, S Reservoir offer: In every eline single-channel and multichannel box, a free single-chamber reservoir will be included free of charge** For additional info on the Biohit reservoirs, please see page channel µl* % 0.80 % 0.15 % 0.20 % 0.40 % J * 2 Note: Minimum volume in P-mode is 500 µl. The 100 µl is possible in d-mode. The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice.finas Accredited Calibration Certificate (ISO 17025) available for new pipettes see page 59 for details **Biohit reserves the right to modify or substitute the free reservoir offer, or discontinue without notice. **Biohit reserves the right to modify or substitute the free reservoir offer, or discontinue without notice. Pipetting Academy on page 70 Biohit Liquid Handling Products 25

26 Clear LCD-display informs and instructs the user during pipetting Both left- and righthanded operation Easy-to-use keypad enables fast programming Unique electronic onetouch tip ejection Volume range color coding for easy pipette and tip selection Dispensing head rotates to both directions without accidental disassembling for optimum pipetting convenience Individual piston/tip cone assemblies allow easy repair and maintenance NEW! Ergomate on page 55 Effortless Tip Loading Optiload tip loading mechanism Ejection & Optiload tip loading and perfect tip sealing All eline multichannel models are equipped with spring loaded tip cones - the Optiload mechanism developed by Biohit Allows tip loading with an equal constant force to every channel Enables perfect tip sealing to every individual tip cone Replaceable Safe-Cone Filters help prevent contamination of the pipette and samples Autoclavable dispensing head (excl µl 8/12 models) e Tips on pages Filters on page 36 Stand options available on page 58 Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 Autoclaving instructions on page 88 FREE! Reservoir offer: In every eline single-channel and multichannel box, a free single-chamber reservoir will be included free of charge** For additional info on the Biohit reservoirs, please see page 56. eline Electronic Multichannel Performance specifications and ordering details Cat. No Channels 8-channel 8-channel 8-channel 8-channel 12-channel 12-channel 12-channel 12-channel Volume Range (μl) µl µl µl µl µl µl µl µl Increment (μl) Test Volume (μl) Inacc. (%) 0.90 % 4.00 % 0.50 % 0.70 % 2.00 % 0.50 % 0.70 % 2.00 % 0.50 % 2.50 % 0.90 % 4.00 % 0.50 % 0.70 % 2.00 % 0.50 % 0.70 % 2.00 % 0.50 % 2.50 % Impr. (%) 0.50 % 0.80 % 3.00 % 0.20 % 0.30 % 0.20 % 0.30 % 0.20 % 0.30 % 0.50 % 0.80 % 3.00 % 0.20 % 0.30 % 0.20 % 0.30 % 0.20 % 0.30 % Safe-Cone Filters Standard Plus Tip ID A, L, M C, D, O D, Q * 1 H, T, Z A, L, M C, D, O D, Q * 1 H, T, Z 26 Biohit Liquid Handling Products * 1 Note: Maximum volume 300 µl. In the d-mode the total displayed volume can not exceed 270 µl. * 2 Note: Minimum volume in P-mode is 500 µl. The 100 µl is possible in d-mode. The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. FINAS Accredited Calibration Certificate (ISO 17025) available for new pipettes. **Biohit reserves the right to modify or substitute the free reservoir offer, or discontinue without notice.

27 NEW! The eline PCR Starter Kit includes: eline μl single-channel pipette eline μl single-channel pipette eline Lite electronic positive displacement dispenser Charging carousel for 4 pipettes SafetySpace 10 μl Filter Tip rack, sterile eline PCR Starter Kit Cat. No eline Starter Kit offers an easy start in PCR applications and all general laboratory work. This kit provides pipettes for all standard pipetting volumes in PCR, contamination-free and safe PCR processes, accurate and precise work, and user-independent results. SafetySpace 300 μl Filter Tip rack, sterile Dispenser Tip 0.5 ml (10 pcs), sterile Dispenser Tip 1 ml (10 pcs), sterile Aluminium cooling rack for 12 micro tubes Cryo pen (black) Literature Pipettes eline operation modes Pipetting (P) Reverse Pipetting (rp) Pipetting with Mixing (P+Mixing) Pipetting with Cycle Counter (P count) Manual Pipetting *1 (P manual) Multiple Dispensing (d) Diluting (dd) Diluting with Mixing (dd+mixing) Sequential Dispensing (Sd) Multi-Aspirating (SA) Automatic Multiple Dispensing (Ad) 6 memory places for your favorite pipetting protocols 9 speeds for both aspiration and dispensing For pipetting modes, please go to page 35. *1 Note: Not available in eline multichannel models. Direct charging feature allows pipetting while charging Cat. No. Item eline Charging Stand for one pipette, with Universal AC-adaptor* eline Charging Carousel for 4 pipettes, with Universal AC-adaptor* eline is the perfect pipette taking care of both the work and the person using it Lisbeth Lemmetti, Development Analyst, Biomedicine Blood Centre at Sahlgrenska University Hospital, Sweden Linear Stand eline Replacement Battery * incl. Euro, US/Jpn, UK and China plugs Unique one-touch electronic tip ejection Tip ejection can be laborious and at worst a contributing factor to Repetitive Strain Injury (RSI). With the eline this is no longer the case, as the unique electronic tip ejection system allows tip removal at the light touch of a button. The eline is the only pipette in the market that offers electronic tip ejection. This feature has been designed for both left- and right-handed operation. UNIQUELY BIOHIT! Patented Biohit DC-motor concept The patented and unique Biohit concept includes the DC-motor, solenoid brake and error control. Improved accuracy and precision The solenoid brake of the eline enables over 10 times more precise positioning of the piston compared to a stepper motor driven system. This is a major advantage in multi-dispensing mode where liquid column has to be cut sharply to have even doses. This means superior accuracy and precision. Built-in error control for more reliable results Even the best pipette may occasionally make errors. Important is to be able to notice them. Biohit electronic pipettes feature a built-in error control. The pipette controls and monitors the piston movement in real time. If an error occurs while pipetting, the pipette notifies the user, thus allowing for more reliable results. WATCH VIDEO ON Biohit Liquid Handling Products 27

28 Proline IVD The original electronic for all pipetting needs In 1991, Biohit introduced its first electronic pipettes. Since then, Biohit Proline electronic pipettes have offered effortless and rapid pipetting with high levels of accuracy and precision. Some say that the Proline range offers complete solutions for all pipetting needs. Yes, we think that too. Proline pipettes are ergonomically designed and the tip ejection is easily operated with four fingers to avoid strain and fatigue during pipetting. Proline electronic is available in single- and multichannel models, covering the volume range of 0.2 to 5000 µl. 28 Biohit Liquid Handling Products

29 Pipettes Induction charging provides corrosion-free mounting Feather-touch button activates all pipetting actions and reduces hand fatigue Simple keypad allows easy, error-free selection of liquid handling modes, volumes and speeds Proline electronic for all pipetting needs Lightweight ergonomic design Single-point calibration for special liquids 5 speeds for aspiration and dispensing Wide volume range Design that helps prevent Repetitive Strain Injury (RSI) Option of single- or 4-place carousel charging stands (either needed for operation) Comprehensive range of the most important liquid handling protocols (see page 31) Compound lever design and side position gives easy tip ejection Ejector collar and tip cone can be removed for easy cleaning and maintenance Computerized drive system with fast DC-motor lets you work more efficiently than with mechanical pipettes Requires charging stand Tips on pages Filters on page 36 Stand options available on page 58 Durable tip cone materials provide excellent chemical resistance e Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 Models > 250 µl offer replaceable Safe-Cone Filters to help prevent contamination and damage Proline Electronic Single-Channel Performance specifications and ordering details Cat. No. Channels Volume Range (μl) Increment (μl) channel µl channel µl channel µl channel µl channel µl channel µl 5.00 Test Volume (μl) Inacc. (%) 0.90 % 2.50 % % 0.40 % 0.70 % 2.00 % 2.50 % 0.40 % 0.60 % 2.00 % 2.00 % 0.40 % 0.70 % 9.00 % 0.60 % 0.80 % 2.00 % 2.00 % 0.40 % 0.70 % 2.00 % 2.00 % Impr. (%) 0.50 % 0.80 % 1.80 % % 0.15 % 0.30 % 1.80 % 0.15 % 0.20 % 0.15 % 0.20 % 0.80 % 2.00 % 0.15 % 0.20 % 0.60 % 0.15 % 0.20 % 0.60 % Safe-Cone Filters Standard Plus Tip ID - - A, B, L, M - - C, O - - C, D, Q E, G, R E, G, S H, T, Z FREE! In every Proline Electronic singlechannel and multichannel box, a free single-chamber reservoir will be included free of charge** For additional info on the Biohit reservoirs, please see page channel µl * % 0.80 % 0.80 % 0.15 % 0.25 % 0.60 % J The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. *1 Note: Minimum volume in P-mode is 500 µl. The 100 µl is possible in d-mode. Note: Proline electronic pipette needs a charging stand for operation. FINAS Accredited Calibration Certificate (ISO 17025) available for new pipettes. **Biohit reserves the right to modify or substitute the free reservoir offer, or discontinue without notice. Biohit Liquid Handling Products 29

30 Clear LCD keeps you informed of the pipette s current task Feather-touch button activates all piston actions Tip ejector allows effortless tip ejection even in 12-channel models Computerized drive system with fast DC-motor lets you work more efficiently than with mechanical pipettes Individual piston/tip cone assemblies allow easy repair and maintenance Dispensing head rotates for optimum pipetting convenience Models > 10 µl have replaceable Safe-Cone Filters to help prevent contamination and damage Requires charging stand Tips on pages and 12-channel pipettes are available e Filters on page 36 Stand options available on page 58 Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 Proline Electronic Multichannel Performance specifications and ordering details Cat. No. Channels Volume Range (μl) Increment (μl) Test Volume (μl) Inacc. (%) Impr. (%) Safe-Cone filters Standard Plus Tip ID channel µl % 4.00 % 0.50 % 0.80 % 4.00 % - - A, B, L, M channel µl % 0.70 % 2.50 % 4.00 % 0.20 % 0.30 % 2.50 % C, D, O, P, Q FREE! In every Proline Electronic singlechannel and multichannel box, a free single-chamber reservoir will be included free of charge** channel µl channel µl channel µl % 0.60 % 0.50 % 3.00 % 8.00 % 0.90 % 4.00 % 0.15 % 0.20 % 0.15 % 0.20 % 1.20 % 0.50 % 0.80 % 4.00 % D, Q H, T, Z - - A, B, L, M For additional info on the Biohit reservoirs, please see page channel µl % 0.80 % 2.50 % 4.00 % 0.20 % 0.40 % 2.50 % C, D, O, P, Q channel µl % 0.60 % 0.15 % 0.20 % D, Q channel µl % 3.00 % 8.00 % 0.15 % 0.20 % 1.20 % H, T, Z The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. Note: Proline electronic pipette needs a charging stand for operation. **Biohit reserves the right to modify or substitute the free reservoir offer, or discontinue without notice.z 30 Biohit Liquid Handling Products

31 Pipettes Proline charging options 1-Place Charging Stand 4-Place Charging Carousel The Proline electronic pipettes can be charged with a 1-place charging stand or a 4-place charging carousel. The compact design of the 4-place charging carousel is ideal for saving bench space in the laboratory and allows the pipette to be rested between dispensing routines. The 4-place rotating head provides easy access to the desired unit. Stands & Accessories Cat. No. Item Proline Charging Stand for one pipette* Proline Charging Carousel for 4 pipettes* Proline/ePET Replacement Battery * Incl. Universal AC-adaptor (with Euro, US/Jpn, UK and China plugs) Proline operation modes Pipetting (P) Pipetting with mixing (P+Mixing) Reverse Pipetting (excl and 5000 models) (rp) Multiple Dispensing (d) Diluting (excl. multichannel 1200 model) (dd) Diluting with mixing (excl. multichannel 1200 model) (dd+mixing) Sequential Dispensing (only 1200 and 5000 models) (Sd) Multi-Aspirating (only 1200 models) (SA) For pipetting modes, please go to page 35. User-independent accuracy with Biohit electronic pipettes While experience and good pipetting practice are essential for reproducible results with mechanical pipettes, the electronic pipettes produce precise results regardless of user experience. Proline, like all other Biohit s electronic pipettes, features the patented DC-motor concept, which enables more precise and smooth piston movement compared to mechanical pipetting. This means superior accuracy and precision, in all pipetting actions. Results are always reproducible even with small volumes independent on experience, as hand control is totally eliminated. Nine persons were divided into three groups according to their experience in pipetting. Each person pipetted distilled water with the same Biohit Proline mechanical and electronic pipettes (using P-mode). The imprecision of ten subsequent results of volumes 10, 25, 50, 100, 200 and 1000 µl was recorded and the results are detailed in pictures on the right. Imprecision (µl) Imprecision (µl) Imprecision (µl) The Effect of User Experience on Pipetting Precision Experts Volume (µl) Moderate experience Volume (µl) No experience Volume (µl) Biohit Liquid Handling Products 31

32 IVD The compact and economical alternative epet Biohit epet electronic pipettes offer an easy and affordable access to electronic pipetting. By using a direct charging system which requires no charging stand, epet is a very costeffective choice for electronic pipetting! The epet is available in single-channel models covering the volume range of 0.2 to 5000 μl and multichannel models from 0.2 to 1200 μl. 32 Biohit Liquid Handling Products

33 Pipettes Clear LCD display keeps you informed of the pipette s current task Direct charging using AC-adaptor Simple keypad allows easy, error free selection of liquid handling modes, volumes and speeds Feather-touch button activates all piston actions Compound lever design and side position provides easy tip ejection Volume range color coding Computerized drive system with fast DCmotor lets you work more efficiently than with mechanical pipettes epet compact and economical Pipetting (P) Pipetting with Mixing (P+Mixing) Multi Dispensing (d) Diluting (dd) Diluting with Mixing (dd+mixing) 5 speeds for aspirating and dispensing Direct charging makes the epet a costeffective alternative for electronic pipetting Requires no charging stand Ejector collar and tip cone can be removed for easy cleaning and maintenance Tip cone design allows easy tip mounting from trays and visual seal verification Models >100 µl offer replaceable Safe-Cone Filters to help prevent contamination and damage Tips on pages Stands & Accessories e Filters on page 36 Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 Cat. No. Item epet Stand for one pipette Linear Stand Proline/ePET Replacement Battery epet Electronic Single-Channel Performance specifications and ordering details Cat. No. Channels Volume Range (μl) Increment (μl) Test Volume (μl) Inacc. (%) Impr. (%) Safe-Cone Filters Standard Plus Tip ID channel µl channel µl channel µl channel µl channel µl * % 2.50 % % 0.40 % 0.70 % 2.00 % 2.50 % 0.40 % 0.70 % 2.00 % 0.40 % 0.70 % 2.00 % 2.00 % 0.50 % 0.80 % 0.80 % 0.50 % 0.80 % 1.80 % % 0.15 % 0.30 % 1.80 % 0.15 % 0.20 % 0.50 % 0.15 % 0.20 % 0.60 % 0.15 % 0.20 % 0.30 % - - A, B, L, M - - C, O E, G, S H, T, Z J FREE! In every epet single-channel and multichannel box, a free single-chamber reservoir will be included free of charge** For additional info on the Biohit reservoirs, please see page 56. Includes Universal AC-adaptor (incl. Euro, US/Jpn, UK and China plugs) The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. *1 Note: Minimum volume in P-mode is 500 µl. The 100 µl is possible in d-mode. FINAS Accredited Calibration Certificate (ISO 17025) available for new pipettes. Biohit Liquid Handling Products 33

34 epet Electronic Multichannel Pipettes Direct charging feature allows pipetting while charging Clear LCD-display keeps you informed of the pipette s current task Tip ejector allows effortless tip ejection even on 12-channel models Volume range color coding Dispensing head rotates for optimum pipetting convenience 8- and 12-channel pipettes are available Individual piston/tip cone assemblies allow easy repair and maintenance epet multichannel pipette family Requires no charging stand Tips on pages Filters on page 36 Safe-Cone Filters for models > 10 µl Durable tip cone materials provide excellent chemical resistance e Ergomate on page 55 Ergonomics on page 70 Pipette selection guide on page 7 FREE! In every epet single-channel and multichannel box, a free single-chamber reservoir will be included free of charge** For additional info on the Biohit reservoirs, please see page 56. epet Electronic Multichannel Performance specifications and ordering details Cat. No. Channels Volume Range (μl) Increment (μl) channel µl channel µl channel µl channel µl channel µl channel µl channel µl channel µl 10.0 Test Volume (μl) Inacc. (%) 0.90 % 4.00 % 0.50 % 0.70 % 2.50 % 4.00 % 0.40 % 0.60 % 0.50 % 3.00 % 8.00 % 0.90 % 4.00 % 0.50 % 0.80 % 2.50 % 4.00 % 0.40 % 0.60 % 0.80 % 3.00 % 8.00 % Impr. (%) 0.50 % 0.80 % 4.00 % 0.20 % 0.30 % 2.50 % 0.15 % 0.20 % 0.15 % 0.20 % 1,20 % 0.50 % 0.80 % 4.00 % 0.20 % 0.40 % 2.50 % 0.15 % 0.20 % 0.15 % 0.20 % 1.20 % Safe-Cone filters Standard Plus Tip ID - - A, B, L, M C, D, O, P, Q D, Q H, T, Z - - A, B, L, M C, D, O, P, Q D, Q H, T, Z Incudes Universal AC-adaptor (incl. Euro, US/Jpn, UK and China plugs) The specifications are type test specifications achieved under strictly controlled conditions (ISO 8655). Due to Biohit s continuous R&D, specifications may change without prior notice. **Biohit reserves the right to modify or substitute the free reservoir offer, or discontinue without notice. 34 Biohit Liquid Handling Products

35 Pipettes Pipetting modes for Biohit electronic pipettes To see which pipette models offer these features, go to Selection Guide on page 7 Pipetting (P) Automatic blow-out Pipette a selected volume with automatic blow-out with complete control of the piston movement. Post-delivery mixing may be used in conjunction with this mode. Sequential Dispensing (Sd) Program up to 12 aliquot volumes per aspiration A series of different volumes can be delivered in any desired order. An extremely beneficial feature when performing dilution series. Reverse Pipetting (rp) Optimizes performance when pipetting viscous or biological fluids A selected volume plus an excess is aspirated into the tip. After delivery the excess volume remains in the tip and is discarded. Automatic Dispensing (Ad) Efficient automatic dispensing with user-defined pace control This mode is similar to the multiple dispensing mode but has the additional benefit of the dispensing cycles being under automatic control at a user specified pace ( seconds). Multiple Dispensing (d) Quickly dispenses equal volumes Aspirate the total volume plus an excess and repetitively dispense equal volumes of liquid. The volume and number of remaining aliquots are shown on the display. Multi-Aspirating (SA) Manual method for washing microplate strips Select the volume and the number of aspirations until the series is completed, then discard the full aspirated volume in a single step. Diluting (dd) Dilution ratios 1:2 to 1:50 Two different solutions separated by an air gap are aspirated and then dispensed together with automatic blow-out. This mode may be used also in conjunction with post-delivery mixing. Post Delivery Mixing (* or + Mixing) User controlled mixing time The piston is automatically moved up and down to mix the liquid in the delivery vessel. The mixing time is controlled by the start button. The mixing function is available in the pipetting or diluting modes (P, dd). Biohit Liquid Handling Products 35

36 Safe-Cone Filters Why should you use Safe-Cone Filters? These unique and replaceable polyethylene (PE) filters act as a final barrier to prevent any fluids and liquid vapors from reaching the internal components of the pipette. Protect the pipette and sample from contamination Prolong the pipette s lifetime Reduce maintenance intervals Cost-effective When should you use them? The ultimate pipette protectors are available in two types: Standard Filter For general applications. Can be used in same type of work as the Plus filter is recommended for, but needs to be changed more frequently. Plus Filter For more demanding applications such as radioactive work, cell culture, bacterial and virological work and molecular biology. cfu Filter No filter 10x 100x 250x 500x Number of pipettings Pipette contamination in pipette barrel when pipetting liquid culture of bacteria Micrococcus Luteus. No. of Pipettings No Filter Safe-Cone Filter +: DNA (50 µl plasmid DNA 120 µg/ml) contamination in pipette barrel. For more info, see literature on and page 94. How often should you change? The interval of changing the filter depends completely on the application and the sample. However, according to studies the filter is recommended to be changed daily (after 50 to 250 pipettings) and immediately in case of overaspi ration. How to change? To ensure the safety of the user, forceps should be used to avoid touching the contaminated filters by hand or use unique built-in filter ejector in mline. In addition, clean the tip cone with ethanol (70%) prior to the assembly of a new filter. Change daily! Forceps included in each pipette package (excl. mline) Safe-Cone Filters Ordering details Cat. No. Description Qty/Unit Standard ø 2.51 mm PE Standard ø 3.15 mm PE Standard ø 5.33 mm PE Standard ø 6.73 mm PE Standard ø 1.83 mm PE UNIQUELY BIOHIT! 50 Filters 50 Filters 50 Filters 50 Filters 50 Filters Plus ø 2.51 mm PE 50 Filters Plus ø 3.15 mm PE 50 Filters Plus ø 5.33 mm PE 50 Filters Built-in filter ejector in mline PE=polyethylene Plus ø 6.73 mm PE 50 Filters 36 Biohit Liquid Handling Products

37 Optifit Pipette Tips

38 Fully autoclavable IVD Environmentally friendly material and manufacturing Biohit Optifit Tips - the perfect match for your pipette Biohit offers you precision-made pipette tips for use with all Biohit and most other pipette makes providing excellent reproducibility and accuracy. You will find these virgin polypropylene tips in single trays as non-sterile or pre-sterilized, in refills and in bulk packaging. And last but not the least, Biohit tips are manufactured in a protected, ISO 8 Clean Room environment, following Good Manufacturing Practices (GMP), causing as little environmental loading as possible. 38 Biohit Liquid Handling Products

39 Optifit Pipette Tips Biohit Optifit Tips In liquid handling, the pipette and the tip always form a system. Biohit tips have been developed to perfectly match Biohit pipettes. By developing and producing both pipettes and tips Biohit is able to offer the best possible compatibility and pipetting results. For example, the Optiload tip loading mechanism developed by Biohit in mline, Proline Plus and eline pipettes allows tips to be loaded with constant force, which, in turn, secures optimal tip sealing and minimum tip ejection force. Optiload tip loading mechanism for perfect tip sealing and light tip ejection Effortless Tip & Loading Ejection As the tip is an integral component of the pipetting system, its shape, material properties and fit have a considerable influence on pipetting accuracy. Variation in any of these will cause errors in the volume pipetted. Best fit - best possible accuracy Perfect fitting and sealing with Biohit pipettes secures the best possible accuracy and precision Ergonomic and light tip attachment and ejection with Optiload in Biohit eline, mline and Proline Plus pipettes Color coding of the tip trays allows for easy matching with a suitable color coded Biohit pipette Compatible with most other pipette makes (see compatibility with other manufacturers pipettes at Providing for all needs of pipetting Both non-filtered and filtered tips available SafetySpace Filter Tips - whenever extra space is needed between the sample and the filter Innovative and ecological packaging choices All non-filtered tips, trays and racks are autoclavable Premium quality and purity Strict quality standards, ISO 9001 and ISO 13485, followed from R&D to production and delivery CE/IVD certified Manufactured in ISO 8 classified clean room conditions The highest quality virgin polypropylene used as raw material DNase, RNase and endotoxin free manufacturing process - Single Trays and Refill Packs certified safe by lot number Pre-sterilized tips are e-beam irradiated All tips, including individual racks, are lot numbered for full traceability Packaging options on page 41 Biohit Liquid Handling Products 39

40 Non-Filtered & Filtered Tips Optifit Tips, non-filtered Biohit s non-filtered Tips are made of premium-grade virgin polypropylene. The tips are metal-free and available in autoclavable (121 C; 20 min; 1 bar) Single Tray racks (as certified RNase, DNase and endotoxin free), in space saving Eco Refill System and in Bulk packages. Electron beam pre-sterilized tips in racks and Refill Packs are also available. SafetySpace Filter Tips for safer pipetting and better accuracy Biohit s SafetySpace Filter Tips are made of virgin polypropylene and feature filter (PE) barriers to prevent aerosol and liquid contamination. This helps protect against the risk of cross-contamination and reduces maintenance requirements of the pipette. SafetySpace Filter Tips have more space between the sample and the filter than conventional filter tips. With SafetySpace Filter Tips you do not need to worry about the sample touching the filter whatever pipetting technique or type of liquid you re using. Especially useful in the following applications which require extra volume in the tip: pipetting foaming liquids like buffers and proteins pipetting solvents multiple dispensing functions of electronic pipettes reverse pipetting Ideal tool in molecular biology microbiology cell culture applications radioactive work The tips are packed in color-coded single tray racks. They are certified DNase, RNase and endotoxin free and are e-beam pre-sterilized. 40 Biohit Liquid Handling Products

41 Packaging options Single Tray racks - for safer laboratory work Material used in tips, trays and racks (polypropylene) is 100% recyclable Fully autoclavable (121º, 20 min) racks can be reused with Refill Tips Air-tight plastic wrapping for maximal purity - wrapping is regular waste Flip-top boxes can be easily opened and closed using a single hand Color coding of the tip trays allows for easy matching with a suitable Biohit pipette RNase, DNase and endotoxin free certified by lot Number. Also available as e-beam pre-sterilized Filter Tips available as pre-sterilized only (non-autoclavable) New rack labels indicate tip volume, lot number, etc. Optifit Pipette Tips Your ecological choice! Eco Refill System Refill Towers - ecological and space-saving ValuePak tips - The new Refill System from Biohit is environmentally friendly with completely recyclable packaging. The tip trays are easily transferred from a space-saving tower or refill pack to the tip boxes. Covers the most popular, non-filtered tips sizes: 10 µl, 300 µl and 350 µl Single Refill Packs - ecological and contamination free Material used in tips, trays and containers are recyclable - container lid is regular waste Compatible with Single Tray racks Individually packed Single Tray racks DNase, RNase and endotoxin free certified by lot number Also available as e-beam pre-sterilized Covers most tip sizes: 10 µl, 300 µl, 350 µl, 1000 µl 1200 µl and 1200 µl extended Bulk package - economic option Completely recyclable Available for non-filtered tips in boxes with 100, 400 or 1000 tips Packed in plastic bag inside the cardboard box Manufactured according to the same quality requirements as all other Biohit Tips Environmental facts: Materials are recyclable Plastics can be incinerated as energy waste Cardboard packaging is renewable Eco Refill System is space saving and produces less waste Environmentally friendly, modern manufacturing technology is used Effective use of raw material Less waste generated Up to 61% less waste by using Refill Tips instead of racked tips Biohit Liquid Handling Products 41

42 Tip Specifications Optifit Tips, non-filtered Tip Tip ID Volume Range Length Packaging Purity Level Qty/Unit Cat. No. Extended A µl 31.5 mm Single Tray Single Tray Refill Tower Refill Pack Bulk in Box ValuePAK B mm Single Tray Single Tray RNase, DNase, endotoxin free Pre- Sterilized 10 x x x x x x x Wide Bore Tip C µl 51 mm Single Tray Single Tray Refill Tower Refill Pack Bulk in Box ValuePAK D µl 54 mm Single Tray Single Tray Refill Tower Refill Pack Bulk in Box ValuePAK E µl 71.5 mm Single Tray Single Tray Refill Pack Refill Pack Bulk in Box Bulk in Box ValuePAK G µl 68.5 mm Single Tray Single Tray Bulk in Box 10 x x x x x x x x x x x x x x x x x H µl 71.5 mm Single Tray Single Tray Refill Pack Refill Pack Bulk in Box ValuePAK 10 x x x x x Extended Z µl 90 mm Single Tray Single Tray Refill Pack Refill Pack J µl 150 mm Single Tray Single Tray Bulk in Box Bulk in Carton 10 x x x x Extra long Tip for XL and MidiPlus K ml 242 mm Bulk in Box U 1-10 ml 161 mm Bulk in box Biohit Liquid Handling Products

43 Optifit Pipette Tips SafetySpace Filter Tips Tip Tip ID Volume Range Length Packaging Purity Level Qty/Unit Cat. No. RNAse, DNase, endotoxin free Pre- Sterilized L µl 32 mm Single Tray 10 x N µl 51 mm Single Tray 10 x O µl 51 mm Single Tray 10 x RNase, DNase & Endotoxin Certificates for Single Tray Tips can be downloaded using the lot number* from P µl 54 mm Single Tray 10 x * available for lots manufactured after June 2005 Q µl 52.5 mm Single Tray 10 x S µl 78 mm Single Tray 10 x T µl 90 mm Single Tray 10 x For your guidance the tips are illustrated at actual size. Filter Tips with regular air gap Tip Tip ID Volume Range Length Packaging Purity Level Qty/Unit Cat. No. RNAse, DNase, endotoxin free Pre- Sterilized M µl 46 mm Single Tray 10 x R µl 78 mm Single Tray 10 x Empty Tip Boxes for Refill System (tips and trays are not included) Item Tip ID Tip Type (Standard Tips) Qty/Unit Cat. No. Empty Tip Box for Refill System A, C, D 10, 300, 350 µl Empty Tip Box for Refill System E, H 1000, 1200 µl Biohit Liquid Handling Products 43

44 Electronic Pipettes eline Single Channel 8-Channel 12-Channel Proline Single Channel 8-Channel 12-Channel epet Single Channel 8-Channel 12-Channel Midi Plus Compatibility charts Ext Optifit Tips, non-filtered WB Ext Ext. Cat. No. μl A * B C D E G H Z J K L * M N O P Q R S T ml 10 SafetySpace Filter Tips 10 Ext. ** ** 1000 * Extended inaccuracy and imprecision of the pipettes with pre-sterilized A tips. Contact your VWR Representative ** Filter Tips with regular air gap for tip compatitbility with other manufacturer pipettes 44 Biohit Liquid Handling Products 1200

45 Mechanical Pipettes mline Single Channel 8-Channel 12-Channel Proline Plus Adjustable Volume Single Channel 8-Channel 12-Channel Proline Plus Fixed Volume Single Channel Proline Adjustable Volume Single Channel 8-Channel 12-Channel Proline Fixed Volume Single Channel Cat. No. μl A * B C D E G J U L M N O P Q R S ml ml ml 10 Optifit Tips, non-filtered Ext WB SafetySpace Filter Tips Ext. ** ** 1000 * Extended inaccuracy and imprecision of the pipettes with pre-sterilized A tips. ** Filter Tips with regular air gap Biohit Liquid Handling Products 45

46 Premium tip quality As the tip is an integral component of the pipetting system, its shape, material properties and fit have a considerable influence on pipetting accuracy. To ensure high quality and accurate pipetting we check each tip lot for Geometry and shape of the tip Occurrence of scratches, mechanical fails, air bubbles, plastic debris and impurities Sealing to tip cone Functionality together with a pipette for straight fluid flow Cleanliness of the tip Our quality management system follows not only ISO 9001 and ISO 14001, but also ISO (ISO standard for medical devices), which enables us to give CE / IVD (In Vitro Diagnostics) certification for both pipettes and pipette tips. The tip production also follows ISO standard to fulfill ISO Class 8 cleanroom conditions. ISO 8 Clean Room manufacturing - contamination free tips To avoid contamination from human contact, we have automated the whole manufacturing process. Pure virgin polypropylene (PP) plastic is automatically fed from silos to molding machines. The molding machines and robots that are located in isolated clean cells load the tips automatically to the tip trays and packaging. HEPA filters and higher air pressure are applied for purity within the cell. All Biohit tip racks and Single Refill Packs are individually and automatically packed in air-tight plastic to rule out any danger of contamination. Additionally, our highly experienced and trained personnel is equipped with specially designed clothing, masks, hair nets and gloves to further diminish the contamination risks. An independent laboratory checks each Single Tray and Refill Pack tip lot for RNase, DNase and Endotoxins. The Certificate of Quality can be downloaded at Traceability All Biohit tips are fully traceable. Mould cavity identification markings are printed on each tip, and tip racks and packages are lot numbered. 46 Biohit Liquid Handling Products

47 Dispensers & Tips Biohit Liquid Handling Products 47

48 For the Best Tip Choice Tip G uid e IVD A single innovation for repetitive dispensings eline Pro and Lite Dispensers hand you the most convenient and ergonomic approach to multiple dispensing. The automated liquid handling operation offers accurate and repetitive dispensing also with viscous, infectious and high vapor pressure liquids. The unique TipGuide selects and displays the most suitable tip size, which saves time and eliminates the possibility of an incorrect tip selection. Additionally, a smart feature included in the whole eline product range is the unique electronic tip ejection, which reduces the risk of RSI (Repetitive Strain Injury). 48 Biohit Liquid Handling Products

49 Dispensers & Tips eline Lite & Pro Dispensers New member of the eline family! eline Lite and eline Pro what is the difference? eline Lite Dispenser for ease of use in multiple dispensings eline Lite is designed for Multiple Dispensing (d) mode with the Tip Guide, and it is quick and simple to operate. eline Pro Dispenser for more versatile use eline Pro offers, in addition to the basic Multiple Dispensing (d) mode, a comprehensive range of liquid handling protocols with easy programming (see table below for all available modes). Both Dispensers offer TipGuide automatic guide for correct tip selection Outstanding ergonomics dispensing with minimum force Unique electronic feather light tip ejection Contamination-free dispensing with positive displacement principle Volume range from 1 μl to 50 ml 5 speeds for aspiration and dispensing Direct charging option enabling dispensing while charging Compatibility with eline charging stand and carousel Compatibility with Biohit Dispenser tips and Eppendorf CombitipsPlus tips. eline Lite & Pro Dispenser Operating Modes Operating modes eline Pro eline Lite Multiple Dispensing with the Tip Guide (d) Multiple Dispensing (d) Pipetting (P) Diluting (dd) Sequential Dispensing (Sd) Automatic Multiple Dispensing (Ad) Multi-Aspirating (SA) Custom mode (CST) - GL (select service intervals) - Sr (select the desired tip range) - SET (returns default settings) 5 speeds for aspirating and dispensing Biohit Liquid Handling Products 49

50 For the Best Tip Choice Tip G uid e Save your time, let the TipGuide make the tip choice for you No time consuming volume calculations Just set the desired volume and number of dispens ings, the TipGuide selects and displays the suitable tip size Clear LCD-display with charging indicator Easy-to-use keypad enables fast programming Direct charging option with AC-adaptor with immediate dispensing possibility Both left- and right-handed operation Electronic start button for all pipetting operations Attractive ergonomic design Unique electronic one-touch tip ejection Easy & light tip insertion eline Dispenser Ordering details Cat. No. Item eline Pro Dispenser without adaptor eline Pro Dispenser with adaptor eline Lite Dispenser without adaptor eline Lite Dispenser with adaptor eline Charging Stand, with AC-adaptor eline Charging Carousel for 4 pipettes, with AC-adaptor eline replacement battery 50 Biohit Liquid Handling Products

51 Dispensers & Tips Biohit Mechanical Stepper The Biohit Mechanical Stepper is an easy to use positive displacement dispenser which allows rapid multi-dispensing of pre-set volumes up to 48 times in succession without refilling. The unit comes complete with an adapter for 25 ml and 50 ml tips. Features include: More ergonomic finger support Easy single handed operation New, more ergonomic design with improved finger support Lightweight (105 g) yet robust construction Maintenance-free design Compatible with a wide range of dispenser tips Ideal for dispensing aqueous and viscous liquids Dispensing volumes from 1 µl to 5000 µl (min. volume with Biohit Dispenser Tips is 2 µl) Its ergonomic design and positioning of the dosage button in the upper part of the device allow single-handed volume selection, loading and dispensing. 48 dispensing steps at intervals of 1 second without the need to refill thus saving 90 % of the working time required by the usual pipetting technique Tested according to ISO Biohit Mechanical Stepper and Biohit Dispenser Tip system Performance specifications Tip volume (ml) Dosage volume Imprecision (%) Inaccuracy (%) Adjustment on dispenser Number of steps µl 4 µl 6 µl 8 µl 10 µl < 1.6 % < ± 3.0 % µl 8 µl 12 µl 16 µl 20 µl < 1.3 % < ± 2.0 % µl 20 µl 30 µl 40 µl 50 µl < 0.5 % < ± 0.8 % µl 40 µl 60 µl 80 µl 100 µl < 0.9 % < ± 0.9 % µl 100 µl 150 µl 200 µl 250 µl < 0.4 % < ± 0.8 % µl 200 µl 300 µl 400 µl 500 µl < 0.3 % < ± 0.4 % µl 400 µl 600 µl 800 µl 1000 µl < 0.5 % < ± 0.6 % µl 1000 µl 1500 µl 2000 µl 2500 µl < 0.3 % < ± 0.2 % µl 2000 µl 3000 µl 4000 µl 5000 µl < 0.2 % < ± 0.2 % The data for tips 0.5 and 2.5 ml are based on a measurement carried out with a 100 µl pipette tip. The performance specifications apply to the Biohit Mechanical Stepper and Biohit Dispenser Tips (non-sterile) System. Ordering Details Cat. No. Description Biohit Mechanical Stepper including adapter for 25 ml and 50 ml syringe tips Biohit Liquid Handling Products 51

52 Biohit Dispenser Tips Features: Function according to positive displacement principle Contamination free dispensing no aerosols are formed Made of virgin polypropylene (tip) and polyethylene (plunger) 9 different tip sizes from 0.1 to 50 ml DNA, RNase, ATP and Endotoxin free Pre-sterilized tips are individually wrapped Suitable for use together with eline Pro and Lite Dispensers and Biohit Mechanical Stepper, as well as most other mechanical steppers (Ripette (Ritter), HandyStep (Brand), Minilab 100/101 (HTL), EasyStep) Biohit Dispenser Tip package Ordering details Volume (ml) Package Qty/Unit Cat. No. Starter Kit Includes 20 * 0.5 ml 20 * 1.0 ml 20 * 2.5 ml 20 * 5.0 ml 20 * 10.0 ml 0.1 Non sterile Pre-sterilized 0.2 Non sterile Pre-sterilized 0.5 Non sterile Pre-sterilized 1.0 Non sterile Pre-sterilized 2.5 Non sterile Pre-sterilized 5.0 Non sterile Pre-sterilized 10.0 Non sterile Pre-sterilized 25.0 * Non sterile Pre-sterilized 50.0* Non sterile Pre-sterilized Adapter for 25 and 50 ml tips, 3 pcs./unit * * * Includes 1 adapter/unit eline Pro and Lite Dispenser and Biohit Dispenser Tip system Performance specifications Tip volume (ml) Volume min/max (µl) Increment (µl) Step size max (µl) Test volume (µl) 0.1 1/ / / / / / / / / The performance specifications apply to the eline Dispenser and Biohit Dispenser Tip System. Due to Biohit s continuous R&D, specifications may change without prior notice. Inacc. (%) 1.70 % 0.80 % 0.50 % 0.50 % 0.60 % 0.60 % 0.90 % 0.60 % 0.40 % 0.40 % 0.50 % 0.50 % 0.30 % 0.30 % Impr. (%) 2.00 % 0.50 % 2.50 % 0.50 % 3.00 % 0.60 % 0.60 % 0.20 % 0.40 % 0.20 % 0.50 % 0.15 % 0.20 % 0.50 % 0.15 % 0.70 % 0.15 % Number of dispensings min/max 1/100 1/100 1/100 1/100 1/100 1/100 1/100 1/50 1/50 IVD 52 Biohit Liquid Handling Products

53 Accessories & Instruments Biohit Liquid Handling Products 53

54 NEW! Ergonomically designed ErgoTube UNIQUELY BIOHIT! From the leader in ergonomic design, Biohit ErgoTubes are designed with a patented flip cap, which significantly reduces strain and effort during use. This feature aids in opening the ErgoTubes more safely than traditional micro centrifuge tubes. The ErgoTube meets today s demand for ergonomic, safe, and functional tubes for use in centrifugation, sample prep, boiling, and storage. Biohit ErgoTubes are RNase, DNase and Pyrogenfree and are available in various colors and volume ranges. ErgoTubes are manufactured in the USA. ErgoTube features include: Fully autoclavable at 121 C (250 F), under 15psi for minutes with drying. Remove from autoclave immediately. Excessive exposure to autoclaving heat may cause warping or decreased structural integrity. Unique ergonomic flip cap design to reduce strain and effort during use Up to 40% less force when opening Contamination-free opening Secure for boiling and freezing DNase, RNase, Pyrogen free (batch certified) packaged in tamper-proof re-sealable bags Low retention tubes as per industry standards Molded with an optical grade ultra smooth surface to minimize sample retention and enhance clarity Excellent for centrifugation, sample prep or storage Made of low binding anti-static virgin polypropylene Available in 3 sizes: 0.5, 1.5, and 2.0ml Several colors for easy sample identification Frosted area for marking-id on the cap and side Occupational Health & Safety recently published a study showing how the occurrence of Repetitive Strain Injuries (RSI) in laboratory personnel can be greatly reduced by the use of microcentrifuge tubes that require less force when opening Quantity 0.5 ml ErgoTube 1.5 ml ErgoTube 2.0 ml ErgoTube Clear ErgoTubes, 500/bag Blue ErgoTubes, 500/bag Yellow ErgoTubes, 500/bag Green ErgoTubes, 500/bag Red ErgoTubes, 500/bag Orange ErgoTubes, 500/bag Purple ErgoTubes, 500/bag Amber ErgoTubes, 500/bag Black ErgoTubes, 500/bag Biohit Liquid Handling Products

55 Accessories Ergomate ergonomic pipetting system When pipetting, ISO 8655 recommends touching off samples on the wall of receiving vessels, especially with small volumes. The new Biohit Ergomate removes the need to hold the plate at any specific angle and allows for safe dispensing, resulting in easier, safer, and more precise pipetting. UNIQUELY BIOHIT! Microplate grids for quick identification and sample management Double-sided. Reverse side featuring numbering from When reversed plate holder creates an ideal aerosol barrier for use in hoods or on bench top surfaces Micro Plate Grids Plate holder can be reversed and used for flat surface pipetting or as an incubation station with lid Ideal for safe transporting of plates Pipetting Academy on page 70 Ergonomic angle for proper positioning of wrist and hand for more comfortable pipetting. Cat. No Item Ergo-Mate Starter Kit, includes 1 Ergo-Mate Plate holder, Pack of 30 grids and 1 Anti-Skid mouse pad Ergo-Mate Plate Holders Pack of 3 MK3001 Ergo-Mate Micro Plate Grids double-sided pack of Ergo-Mate grid pack of Ergo-Mate grid pack of 120 Biohit Liquid Handling Products 55

56 Reagent Vessel The autoclavable and durable reagent vessel is made from polypropylene and is chemically resistant to all common reagents. Cat. No. Qty/Unit Capacity ml Manufactured from FDA Grade white polystyrene, which facilitates the viewing of fluid levels. Extra-wide base helps promote stability and eliminates the chance of accidental spillage. Reinforced sidewall construction makes handling easy and prevents the reservoir from collapsing when full. Pouring spouts on all four corners facilitates emptying fluids when pipetting is completed. Reservoirs are graduated to make filling and checking fluid levels accurate and easy Unique nesting design allows easy separation of bulk pack reservoirs. Sterile supplied reservoirs are certified Rnase, Dnase and Pyrogen Free. Single-Chamber Vessels Cat. No. Description Qty Capacity Sterile & Certified 100/case 25 ml Sterile & Certified 200/case 25 ml Non-Sterile, Bulk 100/case 25 ml Sterile & Certified 100/case 50ml Sterile & Certified 200/case 50ml Non-Sterile, Bulk 100/case 50ml Sterile & Certified 100/case 100 ml Sterile & Certified 200/case 100 ml Non-Sterile, Bulk 100/case 100 ml Three-Chamber Vessels Cat. No. Description Qty Capacity Sterile & Certified 100/case 25 ml Sterile & Certified 200/case 25 ml Non-Sterile, Bulk 100/case 25 ml 56 Biohit Liquid Handling Products

57 Accessories BP 800 Microplate Reader The BP 800 Microplate Reader has all the features of a modern microplate photometer providing readily programmed (optional) assay protocols for Biohit diagnostics. With the help of intuitive user interface, new test protocols can be made fast and easily. Extensive on-board data reduction surpasses many personal computer software packages with its extensive curve-fitting, cut-off calculation, data transformation and validation capabilities. In addition to the printer interface, the instrument has a serial interface to be controlled with a personal computer for further processing and distribution of data. The BP 800 is an extremely flexible microplate reader offering: Multiple plate format capability Cat. No. Ordering information BP Microplate Reader BP Microplate Reader with UV option; including 340 nm filter BP Microplate Reader with NB option; reads Terasaki plates and microplates up to 384-well format Extensive on-board data reduction and analysis Storage up to 75 custom assay definitions Ability to save as many as 8 microplate result sets On-board diagnostic protocols for testing of optical and performance specifications BP 808 Microplate Reader The BP808 Microplate Reader has all the features of a modern microplate reader providing ready programmed assay protocols for Biohit Elisa diagnostics. Integrated keypad with LCD-display supported by menu-driven software with extensive on-board data analysis. Eight channel optics provides fast read time for kinetic applications. 8-channel microplate photometer for kinetic measurements Extensive on-board data analysis Includes curve-fitting, transformations and control validation Cat. No. Ordering information BP808 Microplate Reader BP808IU Microplate Reader with Incubator and UV capability; including 340 nm filter Saves 75 custom assays and 8 test results in memory Programmable and variable shaking speed On-board diagnostic testing of optical and performance specifications IU option provides four-zone incubation and UV capability BW50 Microplate Strip Washer As a self-contained and programmable instrument, the BW Microplate Strip Washer encompasses all applications. It allows for full control and precise fluidic delivery from the gentle dripping of a simple squeeze bottle to the full force of a pressure delivery system. The BW 50 Microplate Strip Washer is the perfect companion for Biohit s BP-series readers. The on-board software of the BW 50 was designed to be programmed in the same format as the BP 800/808 Microplate Readers. This commonality allows the laboratory technician to easily develop both washing and data reduction protocols for those analytes of interest. The operation of both products becomes easy and intuitive. Washes single strips or full plates Syringe drive fluid delivery system for precise operation Cat. No. Ordering information BW 50/8 Microplate Strip Washer BW 50/16 Microplate Strip Washer, 16 channel manifold for 384-well microplates 75 custom protocols Autoprime feature Bottom washing and crosswise aspiration Suitable for IA, DNA probe and cellular assays Biohit Liquid Handling Products 57

58 Pipette Stands When the pipette is not in use, it should be stored in an upright position in order to avoid conta mination from dirty benches. Biohit offers stands for all of its pipettes. The stylish Biohit Linear Stand is designed for all Biohit mechanical and electronic pipettes, and especially for mline, Proline Plus, Proline mechanical and epet electronic pipettes. The stand is also compatible with a wide range of other pipette makes. The compact Carousel Stands are available in different models, both for storage only and for charging. Cat. No. Item Biohit Carousel Stand for 6 mechanical pipettes Carousel Stand for 5 Proline mechanical pipettes Linear Stand for all Biohit pipette models Cat. No. Item eline Charging Stand for one pipette* eline Charging Carousel for 4 pipettes* Proline Charging Stand for one pipette* Proline Charging Carousel for 4 pipettes* epet Stand for one pipette Stands for mechanical pipettes * Includes Universal AC-adaptor (incl. Euro, US/Jpn, UK and China plugs) Biohit Carousel Stand Carousel Stand Linear Stand Stands for electronic pipettes eline Charging Stand eline Charging Carousel Proline Charging Carousel Proline Charging Stand epet Charging Stand 58 Biohit Liquid Handling Products

59 Maxi-Volume Liquid Handling Biohit Liquid Handling Products 59

60 High performance pipetting aids for maximum volumes Let the handling of larger volumes still be easy and lightweight by using Maxi-Volume pipetting aids from Biohit! Biohit offers you a wide range of pipetting controllers and fillers: Biofiller is a lightweight and easy-to-use pipette filler; Midi Plus and Midi Pro are battery operated, rechargeable pipetting controllers; Prospenser Bottle-Top Dispensers and Biotrate Digital Burette provide hassle-free and accurate dispensing. 60 Biohit Liquid Handling Products

61 Maxi-Volume Liquid Handling Midi Plus for comfortable large volume pipetting Biohit Midi Plus is a lightweight cordless pipetting controller which allows aspiration from bottles and tubes without the arm and hand elevations required with serological or volumetric pipettes. It fits all commonly used glass or plastic pipettes, but can also be used with the Biohit 5 ml and 10 ml disposable tips. The speed can be fine-tuned by applying varying finger pressure to the operating buttons. Hydrophobic autoclavable filter prevents over-aspiration Precise finger tip control of variable speed ranges Hydrophobic autoclavable filter prevents from overaspiration Linear speed control Low battery warning CE marked 2-year warranty Operating buttons Fits all commonly used glass or plastic pipettes ml IVD Midi Plus Selection guide Feature Pipette types Rechargeable during use Adjustable speed Midi Plus Plastic or glass ml Pasteur pipettes 5 ml and 10 ml Biohit pipette tips Yes Stepless speed control Stepless speed control allows for the aspiration and dispensing speed to be adjusted easily and precisely to suit either large or small volume pipettes. Gravity dispensing Stand Optional adapters for 5 & 10 ml pipette tips Weight Low battery indicator Autoclavable parts Yes Attached support Yes 207 g Yes Nose cone, pipette holder and filter Midi Plus Technical specifications Feature Batteries Charging batteries Battery service life (from empty) AC-adaptor Midi Plus AA, 3 x 1.2 V, 270 mah, NiCd 14 hours for completely empty battery Approx. 7 hours service life when fully charged Output voltage 9 V DC Filters Hydrophobic 0.45 µm Case material Nose cone material Pipette holder ASA + PC PVDF (autoclavable) Silicone (autoclavable) Midi Plus & Accessories Ordering details Cat. No. Item Qty/Unit Midi Plus Pipetting Controller with Universal AC-adaptor Replacement filter, 0.45 µm, autoclavable Replacement filter, sterile, 0.45 µm * 1 Adapter set for 5 ml tip * 1 Adapter set for 10 ml tip Biohit tip 5 ml Biohit tip 10 ml 100 *1 Note: Adapter set includes both the nose cone (PVDF) and the silicon adapter. 1 5 Biohit Liquid Handling Products 61

62 Midi Pro to speed up large volume pipetting The Midi Pro pipette controller delivers an efficient performance with a powerful yet quiet motor to speed up pipetting of larger volumes. The Midi Pro fits the hand comfortably with the pipette holder at an angle ideal for use within a laminar flow or microbiological safety cabinet. Fills a 25ml pipette in under 3 seconds on its fastest setting Rechargeable during use Powerful pump Low battery light Environmentally friendly nickel metal hydride battery Suitable for all types of pipettes 1-100ml Unique stand suitable for bench or wall mounting CE marked 2-year warranty Operating buttons Selection of high or low modes allowing variable aspirate and dispense speeds (with blowout) Midi Pro Selection guide Feature Pipette types Rechargeable during use Adjustable speed Gravity dispensing Stand Optional adapters for 5 & 10 ml pipette tips Weight Low battery indicator Autoclavable parts Midi Pro Plastic or glass ml Pasteur pipettes Yes High and low speed modes Yes Suitable for bench and wall mounting No 180 g (without stand) Yes Pipette holder Unique stand suitable for bench or wall mounting is supplied with the unit Midi Pro & Accessories Ordering details Cat. No. Item Qty/Unit Feature Midi Pro Technical specifications Midi Pro Midi Pro Pipetting Controller with AC-adaptor Replacement filter, 0.45 μm Replacement filter, 0.2 μm Replacement filter, sterile, 0.45 μm 1 1 Batteries Charging batteries Battery service life (from empty) AC-adaptor 2 x 1.2V NiMH 14 hours for completely empty battery cycles Output 75/10 ma DC Replacement silicone pipette holder 1 Filters Hydrophobic 0.45 µm or 0,2 µm The Midi Pro is fitted with a 0.45μm filter and supplied complete with charger, two spare hydrophobic filters (1x 0.45μm and 1x 0.2μm), bench stand and instruction manual. Case material Nose cone material Pipette holder ASA ASA Silicone (autoclavable) 62 Biohit Liquid Handling Products

63 Maxi-Volume Liquid Handling Easy to adjust calibration mechanism Borosilicate glass barrel protected with a transparent polypropylene sleeve Easily removable PTFE piston for cleaning and smooth action Fine adjustment for exact and reproducible dispensing Anti-drip tap Chemically resistant liquid pathway Precision valve mechanism ensures easy priming and minimum waste with no leakage back into the reservoir - the Prospenser stays fully primed all day Bubble free dispensing Prospenser Bottle-Top Dispenser The easy-to-use Prospenser delivers trouble-free and reliable dispensing of liquids including strong acids, alkalis and solvents. Fully autoclavable All models are fitted with an anti-drip safety valve, a feature which guards against drips when the Prospenser is not in use. A fine adjustment mechanism allows for even greater reproducibility for repeat dispensing. Unlike other bottle-top dispensers, the Prospenser s glass barrel can be disassembled from the pedestal for thorough cleaning. This also ensures that in the unlikely event of damage, you can replace the glass barrel as a spare part rather than have to buy a new dispenser. The head can be conveniently rotated 360º for operator safety and comfort. Wide range of adapters are included to fit the most common bottle sizes. Optional extendable delivery jet allows fast and safe dispensing even to narrow tubes. Prospenser Performance specifications and ordering details Cat. No. Item Increment Max Volume Inacc. (%) Impr. (%) Prospenser ml (with 38, 40 and 45 mm adaptors) 0.05 ml 2.5 ml 0.3 % 0.1 % Prospenser ml (with 38, 40 and 45 mm adaptors) 0.1 ml 5 ml 0.3 % 0.1 % Prospenser ml (with 38, 40 and 45 mm adaptors) 0.2 ml 10 ml 0.3 % 0.1 % Prospenser 1 30 ml (with 38, 40 and 45 mm adaptors) 1.0 ml 30 ml 0.3 % 0.1 % Prospenser 1 50 ml (with and 45mm adaptors) 1.0 ml 50 ml 0.3 % 0.1 % Please note that the Prospenser is not supplied with a reservoir Biohit Liquid Handling Products 63

64 Biotrate Digital Burettes 0 30 ml and 0 50 ml The streamlined Biotrate digital burette and dispenser delivers accurate, precise and convenient bottle-top titration, as well as optimum operator safety. Continuous display of volume dispensed with zero reset feature for multiple titrations The head can be conveniently rotated 360º for operator safety and comfort. See-through barrel ensures bubble-free titration. Automatic switch-off without losing the previous titrated volume, when left unattended No dripping when in static mode A new safety pedestal valve allows the Biotrate fluid path to be turned off manually to prevent accidental delivery. Its right-angled spout is also designed to enhance safety in use. Robust outer casing does not move during the priming or dispensing sequences. PTFE plunger and borosilicate glass barrel allow dispensing of aqueous solutions and some aggressive liquids. Powered by a 3.6V lithium battery giving a minimum life of 60,000 three-minute titrations. Automatic low battery voltage is indicated on the large easy-to-read LCD window. Simple user calibration and factory reset for ISO 9000 and GLP requirements Visible borosilicate glass barrel protected with a transparent polypropylene sleeve allows inspection of liquid before dispensing Chemically resistant and autoclavable liquid pathway Bubble free dispensing Anti-drip tap *Bottle Not Included Autoclavable liquid pathway Biotrate Performance specifications and ordering details Cat. No. Item Increment Inacc. (%) Impr. (%) Biotrate 0 30 ml (with 33, 38 and 45mm adapters) 0.01 ml 0.2 % 0.1 % Biotrate 0 50 ml (with 33, 38 and 45mm adapters) 0.01 ml 0.2 % 0.1 % Please note that Biotrate is not supplied with reservoir 64 Biohit Liquid Handling Products

65 Custom-Made Solutions

66 IVD Tailored solutions for customer-specific systems Yes, it is a true story. Biohit is a leading provider of tailor-made (OEM, Original Equipment Manufacture) solutions to customers who require a little more from their liquid handling devices. Thanks to our know-how, inventiveness and experience, we can take care of the whole process from engineering concept to dispatch. Custom-made only for you. 66 Biohit Liquid Handling Products

67 Custom-Made Solutions Biohit OEM Solutions Safe, reliable and easy to use In many applications, such as diagnostic test and analysis systems, the accuracy of the results and reliability of the system are mission-critical. This requires a lot from all components of the system, also the pipette. Biohit places a great emphasis on accuracy, precision, safety and user-friendliness of its products. Its application-specific solutions have been carefully manufactured according to the requirements of international quality standards. As a customer you can rest assured; Biohit solutions allow you to improve not only the safety and reliability, but also the ease-of-use of your system. Custom-made for your needs Biohit has developed different technologies that can be applied in custom-made solutions. These include both electronic and mechanical liquid handling pipette platforms. The Start mline, eline, Proline and the new rline robotic dispenser module families are easy-to-adapt platforms for your system. For more specific needs, Biohit offers tailored solutions based on any of its pipettes. To learn more about Biohit s OEM solutions, contact your VWR Representative. The Start mline is a pipette based on any mline model that is equipped with an on/ off switch and cable. This can be used with coagulation analyzers or any other instruments that require precise timing. Solutions for leading-edge companies Tailor-made solutions are a speciality of Biohit. Several leading-edge companies have for years been implementing variations of Biohit electronic and mechanical pipettes into their systems. Some of these internationally recognized companies have developed very specific applications with the R&D team of Biohit to exploit their own market niche. The RS eline platform enables the integration of the instrument and the pipette. A flexible cable between the instrument and the pipette provides both the power supply and the operational control via RS 232 communication. The solution is based on the Biohit single- and multichannel eline electronic pipettes. The customer base includes global market leaders such as Pentapharm (picture) 3M biomérieux Johnson & Johnson Group companies For example, Pentapharm GmbH in Germany has developed a whole blood Point-of-Care coagulation system, the ROTEM System, with the RS eline as a key component. The system is designed to reduce the risk of transfusion errors in critical surgery such as liver transplant, cardiac or vascular surgery, orthopedic or trauma surgery. Biohit Liquid Handling Products 67

68 Biohit rline The ultimate OEM Dispenser Module The unique rline Robotic Dispenser Module has been developed as an ideal front-end liquid handling tool for robotic sample processing. This technology platform combines numerous unique and innovative features, like electronic tip ejection and Optiload tip pick-up to improve high quality performance and functionality of sample processors and automated instrumentation. The rline Robotic Dispenser Module is offered as OEM platform for tailored products ranging from 0.2 ml to 5 ml as singlechannel and 0.2 ml to 1200 ml as 8-channel. Additionally, demo versions are available for evaluation and feasibility purposes. A range of robotic tips is available on request. The rline platform features and options: Compact, space-saving design Fast and easy installation Zero cross contamination Integrated electronic tip ejector Versatile serial interface (RS-232 & RS-485 & CANBUS) Low voltage, low current consumption Liquid level sensor Micro volume dispensing options For more information on rline and other customer-specific solutions, please contact your VWR Representative 68 Biohit Liquid Handling Products

69 Pipetting Academy

70 Training for better performance, ergonomics and safety Are you concerned with the results of your work due to pipetting practices or RSI? Is your hand, arm or back hurting when pipetting? Have you considered new or updated instruments to improve your pipetting comfort or results? The Pipetting Academy seminar is your key to better ergonomics, safety and pipetting results in your lab. 70 Biohit Liquid Handling Products

71 Pipetting Academy Pipetting Academy - for better health, safety and pipetting results Pipetting should be easy and provide accurate results but often it can be tiring repetition causing RSI and inaccurate test results. WATCH TRAILER ON To overcome these issues and to help you perform better without compromises on your health and safety, we have developed Pipetting Academy seminars with a comprehensive tutorial material package. In the Pipetting Academy seminars you will be able to increase your knowledge about ergonomics, safety and pipetting performance in general. The seminars are created by Biohit s expert staff in cooperation with customers and third party consultants, who have brought together their experience and knowledge to promote better pipetting. To learn more about the Pipetting Academy and to participate, contact your local Biohit representative. Why join the Pipetting Academy seminars? Are you concerned with the results of your work due to pipetting practices or RSI? Is your workstation comfortable and do you have the right posture when pipetting? Have you considered that the pain in your arm and hand may be related to the instruments or techniques you use? Do you know which technique to use with different types of liquids? Do your results vary between different users? Does your equipment comply with tightening quality regulations? At the Pipetting Academy seminars you will, among other things, learn how to recognize the risk factors and how to select the right tools and working postures. The key topics will be delivered through easy-to-understand videos and animations to visualize the important aspects of pipetting techniques and ergonomics at your work. These powerful tools aim to make an impact on your daily work, at your lab! What will you learn? Be guided through the essentials of laboratory ergonomics Gain a better understanding of the ergonomic risks in the laboratory environment and liquid handling in particular Learn how to avoid these risks by choosing the most appropriate working postures, liquid handling devices and accessories Appreciate how you can contribute to savings in both direct and indirect costs that are related to bad ergonomics Better understand the influence of pipetting techniques and environmental factors on the testing results Be able to instruct your employees on all these issues and make work not only more efficient but also more enjoyable Get hands-on training to pipetting techniques that will help you and your co-workers get more accurate and precise results in the lab Seminars, How to attend Biohit Liquid Handling Products 71

72 Choosing the right seminar Pipetting Academy offers you different seminars for different purposes. You may choose among: Seminars include both theory and practice! Ergonomics Learn about the optimal posture for pipetting and be familiar with the tools that can help you work ergonomically and efficiently. Know the risks and see the solutions. Videos and animations support learning Pipetting techniques Master your working tool. Handle the pipette correctly. Be guided through the several techniques your pipette is capable of. Pipette calibration Learn how to calibrate your pipette correctly to gain the most accurate pipetting results. ISO8655 quality standards All you need to know about the standards you face in your everyday work. 72 Biohit Liquid Handling Products

73 Pipetting Academy How to sign up for seminars? To sign up for the seminar contact your local Biohit representative The seminar is held in a location most suitable for you and your colleagues The trainer will be certified by Biohit to hold the Pipetting Academy seminar Each participate will receive a certificate of participation after the seminar Get access to the educational material, videos and animations Once you have signed up and participated the seminar, you will automatically get access to educational videos, animations and presentations about ergonomics, pipetting techniques and calibration. Through the animations you will be guided step by step in correct handling of the pipette and in reverse pipetting, diluting and all other pipetting modes to make your work easier and more efficient. Participate in a seminar and get access to the material on A Certified Professional Ergonomist will guide you through the essentials in Ergonomics. Presentations with explanations and illustrations about calibration and quality standards are also available. NEW: Eligible participants may qualify for special training guide and materials. See you at Pipetting Academy! Get certificate of participation! WATCH TRAILER ON Screenshot from a Pipetting Academy animation Biohit Liquid Handling Products 73

74 Follow the signs of the ergonomic pipette Maximum comfort Did you know that...? Pipetting over 1.5 h/day (300 h/year) exposes to RSI Light weight Good finger support Ergonomic handle design (Repetitive Strain Injury) Lab professionals have twice as much WRULD (Work Related Upper Limb Disorder) as employees in general 44 % of lab professionals suffer from disorders caused by pipetting Pressing the plunger button as well as ejecting tips with a conventional mechanical pipette together require a force up to 70 N (7 kg), which corresponds to the force required to lift 2 house bricks or 7 litres of milk Light plunger movement Light tip loading & ejection Fast & light volume adjustment Optimal length Clear & easy-to-read display mline and eline offer exceptionally light and ergonomic pipetting operations Comparison of pipetting work with electronic and mechanical pipettes Both the tip ejection force and the distance thumb has to travel to eject the tips have been measured. Mechanical Pipettes effortless accuracy Light weight for easy liquid handling Exceptionally light pipetting forces Tip loading and ejection require only minimum force (Optiload) Both left and right handed operation Consistent results time after time For easy and ergonomic manual pipetting, your first choice should be mline as recommended by Health & Safety Officers of leading pharma ceutical companies. Pay also attention to: Weight and design of the pipette Your seating position Rest intervals Bench layout & lighting Number of repeated pipetting series Electronic Pipettes leader in performance & ergonomics Fully electronic pipetting operations Multi-function device a lot more pipetting modes than in a mechanical pipette Less stages means time-savings especially in multi-dispensing (twice as fast as a mechanical pipette) 30 times lighter pipetting than with a mechanical pipette Unique electronic one touch tip ejection Both left and right handed operation User independent accuracy and precision When you need to pipette and dispense liquids as comfortably as possible, your first choice should be eline. This Award Winning pipette enables you to focus on your work instead of worrying about Repetitive Strain Injury. 74 Biohit Liquid Handling Products

75 Pipetting Academy What can be done to enhance safety? Pipetting with air displacement pipettes is one of the most common laboratory tasks and includes a number of potential risks. The risks can be divided into three defined areas: A B 1. Physical risk through stress (ergonomics) 2. Situations where the user is at risk of contamination through handling of infectious or toxic agents 3. Situations where the sample or specimen is at risk through contamination from other samples or environmental factors. Cleanliness is safety - protect your pipette and your sample from contamination The user is often well protected by appropriate working benches, seats, clothing and shields, but the pipette is forgotten. Modern air displacement pipettes with disposable tips enable fast and accurate pipetting, but contamination of the pipettes may increase both the possibility of unreliable results and health risks in laboratory work. Fig A. Biohit mline features a unique tip cone filter ejection mechanism. Fig B. The tip cone filter can be changed easily and safely with the use of forceps supplied with every Biohit pipette (excluding mline). Organic and inorganic contamination can create problems for the scientist. No matter whether the particles are viral, bacterial ribonucleases or metal elements, in the testing environment they may lead to erroneous results. It is the reduction in the numbers of these particles and avoidance of cross contamination or accidental addition to the sample or the equipment being used that needs attention. Corrosion of the pipette piston is another threat. The number of corroded pistons that are replaced at service centers every day, and pipettes with totally contaminated inner parts is surprisingly high (see Figures A & C). Finally, to ensure the safety, the laboratory worker should also pay attention to the ergonomics at work (pipette and environment), take care of the proper shielding and protection (gloves, clothes) and follow good laboratory practice by pipetting slowly and carefully to minimize aerosol formation and foaming. Fig A: Badly corroded and contaminated piston assemblies taken from a commonly used pipette Fig B: Biohit pipette tip cone used with Biohit Safe-Cone Filter Fig C: Tip cone taken from a commonly used brand of pipette after over-aspiration of blood-sample Fig D: Clean tip cone Biohit safety features include Ergonomic pipette design for improved occupational health Accurate and precise results with the appropriate tools Cleanliness through Safe-Cone Filters, certified tips, autoclavable pipettes and decontamination tools Appropriate storage with a wide range of pipettes stands High-quality after-sales services & accredited calibration Biohit Liquid Handling Products 75

76 How to get consistent results? One of the most common activities of laboratory workers is primary sample or reagent addition whilst performing a test. Some laboratory workers perform thousands of tests daily. There are a number of factors that influence the pipetting performance. For example, the quality of the products and tips, human error, pipetting techniques, and strain, as well as other factors, such as environment (temperature, air pressure, humidity), type of sample and type of application. The most difficult demand for accurate and precise pipetting results is the consistent handling and continual depression of the plunger on pipettes. Accuracy It is possible to be very consistent, but consistently wrong. Inaccuracy is the numerical difference between the mean of a set of replicate measurements and the true value. High accuracy, i.e., small inaccuracy means very little difference between the mean sample and the true value. Accuracy is achieved by careful calibration of a precise instrument. Precision Precision is an agreement between replicate measurements. Precision has no numerical value, it is quantified by imprecision. High precision, i.e., small imprecision means very little variation between repeated measurements on the same sample. To achieve it a precision instrument is required, but also good laboratory practice must be followed. Ten times more precise dispensing with Biohit electronic pipettes unbeatable linearity When comparing the stepper motor technology and the DCmotor technology for accuracy and precision, the dispensing (d) mode is the best choice. The DC-motor driven Biohit eline and a stepper motor driven pipette were tested for linearity in the d-mode. The measurement was done 5 times. The DC-motor driven Biohit electronic pipette allows linear movement, - i.e. precise dispensing. The tested stepper motor pipette generated a linear movement error, which is equal to over ± 1.5 µl. This even exceeds the minimum adjustable increment of the pipettes. In the d-mode, where even dosing is important, this kind of an error is significant. Consistent results time after time with Biohit mechanical mline pipettes Performance test of Biohit mline (violet line) and a conventional pipette (blue line). Pipettes were tested over a 5-hour period with sets of 5 repeat weighings of 500 microlitres 180 times. The same trained operator was used. As shown, it is very hard to meet the specifications (red line, ISO ) with a force demanding pipette after prolonged pipetting hours, whereas the light action of mline allows the same reproducibility over the whole period Stepper-motor driven electronic pipette Error (mm) Measuring points Error (mm) DC-motor driven Biohit eline electronic pipette 76 Biohit Liquid Handling Products Measuring points Biohit mline 20 x 50 µl linearity measurement in d-mode.

77 Pipetting Academy Pipetting preparations Use the tip specified by the manufacturer. Ensure that the pipette and the tip have been tested according to ISO 8655 and the tip is seated correctly. Make sure pipettes have been correctly calibrated. Check that the pipette, tip and liquid are all at the same temperature. When pipetting liquids with temperatures different to the ambient temperature, do not pre-rinse the tip. Change the tip after each pipetting. Ensure that any fluid viscosity variations have been accounted for and the correct technique is employed, i.e. reverse pipetting. If handling infectious or radioactive agents make sure appropriate shielding and other precautions protect the operator. For information on safety accessories see pages Use Safe-Cone Filter in the tip cone whenever possible. Biohit Liquid Handling Products 77

78 Pipetting action Hold the pipette in a vertical position. Tilting the pipette at an angle causes a volume greater than the set volume of liquid to enter the tip. Pre-rinse of tip is always recommended. When aspirating fluid the pipette tip should normally be immersed to a depth of 2-3 mm. When using a mechanical pipette, operate piston with a smooth and consistent thumb action for repeated results without foaming or bubbles. It is recommended to pipette against the inside wall of the receiving vessel. Remove the tip by drawing it up against the inside wall. Ensure the pipette blow out action is fully activated. Ensure the volume is still set at the required position. Pipette with volume locking mechanism are recommended to avoid accidental volume change during pipetting. Avoid leaving the pipette on its side with liquid in the tip, which might seep back into the mechanism. Other cautions Rack the pipette when not in use see product pages for more information on pipette stands. Electronic pipettes should be returned to their charging stands. Avoid dropping the pipette or allowing contact with dirt or grease. Change the Safe-Cone Filter regularly (recommendation after 50 to 250 pipetting cycles) and always in case of over-aspiration. Never strike the tip cone against the tip tray when loading the tip as this can damage the pipette. Avoid exposing the unit to extreme temperature changes, humidity and dust (operating temperature from 15 C to 40 C). Service the pipette regularly. Clean the pipette thoroughly before sending to service. Decontaminating the pipette with Biohit Proline Biocontrol or 70% ethanol is a suitable procedure. Notify the service personnel for what purpose the instrument has been used. Postal services can refuse to deliver instruments used with hazardous materials. Make sure that a qualified person services the pipette. 78 Biohit Liquid Handling Products

79 Pipette Calibration & Maintenance

80 Leading edge after-sales services by Biohit Beside pipetting, laboratories must pay attention also to the quality and performance of the tools and equipment that are used in everyday work. To ensure the continuous accuracy and precision of your liquid handling instruments we offer a wide range of calibration, diagnostic, and repair services, as well as preventative maintenance programs. Regular, periodic preventative maintenance (cleaning, lubrication, calibration) will keep your pipette in optimal operating condition and extend the life-span of the pipette. 80 Biohit Liquid Handling Products

81 Pipette MD and Biohit Authorized Service Providers Among the Global Network of Authorized Service Centers and Service Providers is Pipette MD, the technical service division of Biohit Inc. Located within the Biohit Inc. facility in Neptune, NJ, Pipette MD provides calibration, diagnostic, repair, and preventative maintenance services. Biohit Pipette MD Service Pipette MD has the expertise to service and calibrate most makes, models, and volumes volumes of pipettes from 0.1ul to 50ml. Pipette MD staff consists of a group of highly trained technicians with the knowledge and skill to calibrate, diagnose, repair, and perform preventative maintenance on most pipettes. Advantages are as follows: Professional one-stop pipette service for most makes and models On-site/In-shop services for your convenience Easy and simple-to-access service Cost savings through a single service contract for most makes and models Please visit for on-site or in-shop repair and calibration service information. Biohit Authorized Service Providers Biohit Service Providers are independent companies trained and authorized by Biohit/Pipette MD to service and calibrate Biohit liquid handling devices. These service partners provide quality and convenient local service within their geographical areas of operation. The Biohit Authorized Service Providers maintain standard operating procedure for technical service and calibration of Biohit-made products. Please contact your VWR Represenative for your nearest Biohit Authorized Service Provider. Biohit Authorized Service Provider Preventative Maintenance Service Repair Service Calibration Service Performance Testing Full Spare Part Service Product And Service Training Through Biohit After-Sales Services you can expect: High quality maintenance, repair and calibration services for all Biohit pipettes Good operating condition and extended lifetime of the pipettes thanks to scheduled preventative maintenance programs and calibration services Cost savings with original spare parts for Biohit pipettes Time savings through convenient and cost-efficient service contracts and fast turnaround times Detailed documentation on services performed Biohit Liquid Handling Products 81

82 Pipette MD Calibration Services Piston-operated pipettes require testing at regular intervals to insure conformity to established maximum permissible systematic and random errors. (ISO 8655, part 1, page 6, section 7.3). The frequency of the testing and calibration depends upon: Accuracy requirements of the application Frequency of use Number of operators using the pipette Nature of the liquid dispensed Specifications established by the user Requirements from GLP or quality systems Pipette MD offers a complete menu of calibration services using the gravimetric test method in accordance with ISO Pipettes are tested for inaccuracy and imprecision and, if calibration at several volumes is selected, also for linearity. When performing calibration, necessary steps are taken to adjust the inaccuracy and imprecision, if required. Calibration levels and associated costs are determined by the number of volume settings a pipette is to be tested at, as well as the number of measurements to be taken at each volume. Typically a pipette is tested at two or three volume settings: Maximum and 10% of max Maximum, 50% of maximum, and 10% of max 8 Note: To utilize PMD calibration, repair, or preventative maintenance services, contact us at info@ pipettemd.com or toll free at MD-PIPET. Before sending any pipettes to PMD, please ensure they are free from all contamination. PMD requires you to obtain and complete a service packet which contains a decontamination certificate from Pipette MD. 82 Biohit Liquid Handling Products

83 Pipette MD Calibration Services Regardless of the Calibration Level selected, most calibration services can be broken down into the following categories: Calibration Only (No lubrication, no cleaning) As Left Calibration Service As Found and As Left Calibration Service Calibration Only Service: also known as As Found or Pre-Cal service Calibration Only service is performed prior to any work being done to a pipette, in order to guarantee that the pipette was working properly before servicing. Calibration Only service can be utilized with most Calibration Levels, but is typically used with very basic, verification level service such as 2 volumes 3 readings per volume. Pipettes passing the Calibration Only service will be issued a Calibration Certificate. In order to perform Calibration Only service, the pipette must be operational. Non-operational pipettes cannot be tested on a balance and a Certificate cannot be generated. As Left Calibration Service As Left calibration service involves the servicing of a pipette prior to taking readings on a gravimetric balance. Pipettes which are operational upon receipt have the lower end components cleaned and lubricated. Note: If an operational pipette requires new parts in order to generate a passing certificate, those parts will be quoted separately from the As Left calibration service level selected. The pipette is then tested on a balance according to pre-selected Calibration Levels and a certificate is generated. Pipettes which are non-operational are treated as repairs. Parts and/or service required to make a pipette operational will be quoted separately from the Calibration service requested. As Found/As Left Calibration Service Occasionally, some customer pipettes require a combination of As Found (Calibration Only) & As Left service. Pipette MD can perform this combination service and in the process will generate two Certificates for one pipette: an As Found Certificate and an As Left Certificate. Biohit Liquid Handling Products 83

84 Pipette MD Diagnostic and Repair Services Pipette MD can repair most makes, models, and volumes of pipettes on the market. Pipette MD Repair Service What Is Done to the Pipette Benefits Suitable for Inspection of Pipette Troubleshooting/Diagnosis of the problem Disassembling of the pipette: all parts are inspected for damage and wear Components are cleaned or replaced, if necessary Re-greasing of the piston and/or o-ring Pressure leakage test Calibration and performance testing according to ISO 8655 or manufacturer s specifications Detailed service documentation: service repair and calibration report are supplied with each repaired pipette Fully repaired and functional pipette Genuine manufacturer s parts are utilized for customer s pipette repairs Any customers that have pipettes with damaged components or pipettes which are either malfunctioning or nonfunctional, and that need repair and calibration by qualified specialists For more information about Preventative Maintenance, Repair and Calibration services, please contact your local Biohit representative or go to www. pipettemd.com. You will require a Pipette MD service packet and Return Authorization number from PMD in order to send your pipette in for repair. Please contact PMD at info@pipettemd.com or MD-PIPET 84 Biohit Liquid Handling Products

85 Pipette MD Preventative Maintenance Services Liquid handling devices, such as pipettes, are precision instruments, that are comprised of a variety of mechanical and electronic parts. These parts are naturally subject to wear and damage over time, for example: The piston surface can deteriorate, wear, or corrode over time from friction and/or chemical corrosion. Tip cones are subject to chemical corrosion and wear, especially in the tip sealing area. Batteries of electronic pipettes do not last forever and must be replaced periodically. The wear and tear that afflicts all pipettes can be overcome by implementing regular and periodic maintenance and calibration programs. According to Biohit/Pipette MD experience, pipette failure rates can be significantly reduced if a regular and scheduled preventative maintenance program is carried out on the pipettes. In addition, the pipette lifetime can easily be extended through regular service and replacement of critical components. Those components in general are tip cones, pistons, and batteries (electronic pipettes only). Pipette MD offers scheduled Preventative Maintenance Programs for service and calibration. Contact Pipette MD at MD-PIPET or info@pipettemd.com for more information on these programs including pricing. Pipette MD Preventative Maintenance Program What Is Done to the Pipette Benefits Suitable for Cleaning of the pipette exterior Cleaning and/or greasing of the lower parts (ejector collars, piston, seal/o-ring, tip cone) is done when replacement of parts is not required Replacement of the critical parts per preventative maintenance schedule, or as necessary. Critical parts are: piston, tip cone, and battery (electronic pipettes only) Visual leak test for all models; visual linearity test for multichannels. Calibration and performance testing according to manufacturer s specifications. Detailed service documentation: service and calibration report Improved pipette reliability: consistent performance/enhanced accuracy and precision Fewer failures due to unexpected malfunctions of the pipette Decreased pipette downtime as a result of preventative maintenance programs Increased pipette lifespan Fewer number of pipettes that fail asfound testing Laboratories that have a strict quality system requirements such as ISO 9001, ISO 17025, GLP or other quality requirements imposed by local legislation, international standards or product/ service type. Any laboratory that values the integrity of their pipetting data Biohit Liquid Handling Products 85

86 Terms and definitions Preventative MAINTENANCE Preventative maintenance means renewing or replacing critical parts of a device on a regular basis to prevent malfunctions. In other words, preventative maintenance is about maintaining equipment in good operating condition. There is no point in waiting for the pipette to fail. PERFORMANCE TESTING It is important to understand the difference between calibration and performance testing. Performance testing is often thought to be calibration, which is not the case. Performance testing is the verification procedure for the pipette using a gravimetric, photometric or other test method. Performance test is carried out to check that the calibration of the pipette is correct. In other words, the balance reading converted into volume should be in accordance with the selected volume in the display. If the results are incorrect, the pipette will be calibrated and tested again. CALIBRATION According to ISO (2002), calibration is a set of operations that establish the relationship between the dispensed volume and the corresponding nominal or selected volume of the pipette. In other words, calibration is the adjustment of the pipette to give the selected volume. In practice, the length of the piston movement is adjusted so that the balance reading is in accordance with the selected volume on the display. Every pipetting operation is only as accurate as the calibration of the pipette used. ISO 8655 ISO 8655 is a new international standard for piston operated volumetric apparatus. ISO 8655 defines, among other things, terms and definitions regarding piston operated pipettes and their testing, requirements for test equipment and test conditions, testing procedure and maximum permissible systematic and random errors for pipettes (inaccuracy and imprecision). Biohit follows ISO 8655 and takes its requirements into account in its research and development, production, quality assurance and after sales service. GOOD LABORATORY PRACTICE, GLP The Good Laboratory Practice (GLP) principles were created by OECD ( to internationally harmonize test methods and laboratory practices. Today GLP compliance is one of the key indicators of high quality laboratory services. According to the GLP, Apparatus used in a study should be periodically inspected, cleaned, maintained, and calibrated according to Standard Operating Procedures (SOP). Records of these activities should be maintained. Calibration should, where appropriate, be traceable to national or international standards of measurement. For more information, see 86 Biohit Liquid Handling Products

87 Pipette decontamination procedure Mechanical pipettes (mline + Proline Plus) Electronic pipettes (eline) 1. Unscrew the tip ejector collar counterclockwise and remove it. 1. Unscrew the tip ejector collar counterclockwise and remove it. 2. Unscrew the tip cone holder counterclockwise and carefully remove it with the tip cone. Remove the Safe-Cone Filter if fitted. 2. Unscrew the tip cone holder counterclockwise and carefully remove the tip cone holder, tip cone and spring. Remove the Safe-Cone Filter if fitted. 3. Unscrew the piston counterclockwise from the pipette. 3. Unscrew the piston counterclockwise from the pipette. 4. Place the tip ejector collar, tip cone holder, tip cone and tip cone cylinder into a beaker containing Biohit Proline Biocontrol and leave for at least 30 minutes. 5. After performing the procedure described above, remove the components from the beaker and rinse them with distilled water, then dry preferably with warm air, for at least an hour. 6. Re-grease the piston as described in the instruction manual. Replace all components including new filter if fitted. 4. Place the tip cone, tip cone holder, tip ejector collar, piston and spring into a beaker containing Biohit Proline Biocontrol and leave for at least 30 minutes. 5. After performing the procedure described above, remove the components from the beaker and rinse them with distilled water, then dry preferably with warm air, for at least an hour. 6. Re-grease the piston as described in the instruction manual. Replace all components including new filter if fitted. Note: When performing the decontamination procedure as a matter of routine, the o-ring seal should be checked for possible wear at every sixth instance, and replaced if necessary. The calibration should also be checked at the same time. Biohit Liquid Handling Products 87

88 Autoclaving instructions mline & Proline Plus mechanical pipettes The entire mline and Proline Plus mechanical pipette can be steam sterilized by autoclaving at 121 C (252 F), 1 bar (15 p.s.i.) for 20 minutes. The dispensing head of the multichannel pipettes must be unscrewed 360 counterclockwise before autoclaving. Remove the Safe-Cone Filter (if fitted). Put the pipette into the sterilization bag and place it into the autoclave. After autoclaving the pipette must be cooled down and left to dry overnight before use. It is recommended to check the performance of the pipette after every autoclaving, and to grease the piston/seal of the pipette after every 10th autoclaving. Tips and tip boxes Place the bulk tips into the sterilization bag and the tip tray as such in the autoclave. Autoclave for 20 minutes at a temperature of 121 C under 1 bar (15 p.s.i.). Cool down before use. Troubleshooting guide eline electronic pipette, lower part The dispensing head (tip ejector collar, tip cone holder, tip cone, spring and piston) of the single-channel and multichannel models (except for multichannel 1200µl) can be autoclaved (121 C, 1 bar for 20 minutes). These parts can be autoclaved as one unit or separately as individual parts. It is also possible to clean the parts and grease the piston prior to autoclaving. Remove the Safe-Cone Filter (if fitted). Put the dispensing head into the sterilization bag and place it into the autoclave. After autoclaving the parts must be cooled down and left to dry before use. It is recommended to check the performance of the pipette after every autoclaving, and to grease the piston/seal of the pipette after every 10th autoclaving. Note: Excessive heat or length of time can damage the products. Never put handle part of the eline into the autoclave. Lower ends of multichannel pipettes are not interchangeable between 8- and 12-channel pipettes. The cover of the tray should be closed during autoclaving. Problem Cause Solution Pipette performance outside given specs Pipette is leaking Operating button jammed or moves erratically Pipette blocked, aspirated volume too small Tip ejector jammed or moves erratically Unsuitable tip Non-standard test conditions or calibration altered Pipette has not been maintained regularly Dirty Safe-Cone Filter Pipette is leaking Unsuitable tip Tip incorrectly attached Worn or dirty tip cone Worn or dried piston sealing Instrument damaged Liquid has penetrated tip cone and dried inside Safe-Cone filter has been contaminated Insufficient amount of grease on a piston and seal Liquid penetrated tip cone and dried Tip cone and/or ejector collar contaminated Test with original manufacturer s tip Perform test according to the ISO 8655 standard and recalibrate if necessary Perform routine user maintenance and retest Change Safe-Cone Filter See instructions below Use original manufacturer s tip Attach tip firmly Clean tip cone Change tip cone Clean and re-grease o-ring Change the o-ring Send for Service Clean and grease the piston/seal and the tip cone Change Safe-Cone Filter Grease accordingly Clean and regrease o-ring and piston and clean tip cone Clean with soft cloth and mild detergent, Biohit Proline Biocontrol or 70% ethanol 88 Biohit Liquid Handling Products

89 About Biohit Biohit was founded over 20 years ago, in The background of the company, however, stretches back to the 1970s, when Professor Osmo Suovaniemi (M.D, Ph.D.), founder of Labsystems (1971), Eflab (1978) and Biohit (1988) together with his team developed and commercialized the first air displacement, adjustable single and multichannel pipettes that made liquid handling both more convenient and safer than before. These inventions included the first 8- and 12-channel adjustable pipettes that were designed to aid the liquid handling work in the vertical photometry based analyzers, such as the very first 96-well microplate reader (the Titertek Multiskan), invented and developed by Professor Suovaniemi (and his company Labsystems). Over the years, Biohit s liquid handling devices have taken their place in laboratories worldwide as ergonomic, safe and reliable solutions for all types of pipetting and dispensing. Today, Biohit s mission is to enhance the well-being and quality of life of people by developing, manufacturing and marketing liquid handling products and accessories as well as diagnostic test systems for use in research, health care and industrial laboratories. In addition to liquid handling products and accessories, Biohit also develops, manufactures and markets diagnostic test systems for the screening, prevention, and diagnosis of diseases of the gastrointestinal tract. The company s diagnostic tests are combined with pipettes, instruments based on vertical photometry technology, and related software to provide comprehensive analyzing systems for research and clinical diagnostics. Biohit focuses on business areas in which it possesses a strong foundation in research, technology, innovations and patented products. The company s products are designed and manufactured according to the requirements of the ISO 9001, ISO 13485, and ISO quality and environment standards. Biohit also offers accredited pipette calibration according to ISO Additionally, all products are CE/IVD marked. Biohit has several worldwide patents and patents pending for its products. Special pricing available for GSA contract customers - contact your local VWR representative for more information Biohit has a network of 200 distributors, including 8 subsidiaries, in 70 countries. Biohit Liquid Handling Products 89

90 Our high quality standards Biohit s products are developed and manufactured according to the requirements of ISO 9001, ISO and ISO quality and environment standards. The tip production also follows ISO Standard to fulfill ISO class 8 cleanroom conditions. Our accredited pipette calibration follows ISO The products are CE/IVD marked and supplied with individual QC certificates. We aim to continuously develop our products and processes in order to meet and often exceed the demands of regulatory authorities, environmental bodies and most important, our customers. ISO quality standard is specific to medical device quality systems and supplements the more generic ISO 9001 standard that applies to many industries. Additional ISO requirements include those that are critical in the medical device industry, e.g. special processes, design controls, process controls including environmental, traceability, record retention and regulatory actions. ISO 8655 is an international quality standard for the manufacture and testing of piston-operated volumetric apparatus. ISO lists the general competence requirements for carrying out tests and calibrations. The IVD Directive (98/79/EC)1 is the third in a series of European Directives on medical devices. The purpose of the IVD Directive is to ensure that only safe and functional products are sold in the European market, with clearly IVD outlined regulations regarding manufacture, importing, and marketing. The CE/IVD mark stands for excellent quality and purity and thus guarantees maximum reproducibility for experiments and analyses. Tip purity - All tip models available as DNase, RNase and Endotoxin free certified to protect the sample from contamination. Biohit s tip production is ISO 8 cleanroom classified. Warranty Biohit offers 3-year warranty for all mechanical pipettes and 2-year warranty for electronic pipettes. The low lifetime cost of the products, warranty periods and environmentally friendly products allow for high return on investment. FINAS is the Finnish national accreditation body, which operates independently as part of the Measurement Technology Center (MIKES), and who has given Biohit s calibration laboratories in Finland accreditation certificate. An accredited pipette calibration laboratory calibrates pipettes according to precise technical requirements. Our calibration laboratories in Germany, France and the UK have obtained the same status by their national accreditation bodies. Ergonomic Design Improving ergonomics of pipette design and usability to reduce the risk of work related upper limb disorders, such as RSI (Repetitive Strain Injury). Biohit is the global market leader in electronic pipettes, which combine performance and ergonomics to provide accurate, reproducible and safe pipetting in the most demanding liquid handling applications. ISO 9001 ISO ISO ISO Biohit Liquid Handling Products Biohit Liquid Handling Products 90

91 Literature Ergonomics Vaughton, R. (2006) In case of emergency break glass! Int. Labmate. (2006) Mannonen, S., Nieminen, P., Kaasinen, J., Andersin, K. (2004). Multichannel pipetting how to choose the correct pipette? Int. Biotech. Lab. 22(6), and Am. Biotech. Lab 22(13), Mannonen, S., Nieminen, P., Kaasinen, J., Andersin, K. (2003). Raising the standard of mechanical pipetting. Int. Labmate 28(5), Mannonen, S., Hintikka, V. and Syrjä, K. (2002). Choosing the correct electronic pipette. Clin Lab Int. 26(2), 29. Mannonen, S., Hintikka, V. and Syrjä, K. (2002). The benefits of electronic pipetting. How to choose the correct pipette. Int. Labmate 27(4) Guide 2002/2003, Mannonen, S., Hintikka, V., Kaasinen, J. and Ekholm, P. (2002). Reduced tip ejection and pipetting forces prevent repetitive strain injuries (RSI). Biomed. Prod. 4, Vaughton R. (Sep. 1999). Taking the Strain Out of Pipetting. International Biotechnology Laboratory. Hoskins D.B., Erickson J. (1998). Laboratory Ergonomics, the Wake-Up Call: A Case Study on How One Company Relieved Stress and Strain on Its Employees, Chemical Health and Safety, January/ February. David G., Buckle P. (1997). A Questionnaire Survey of the Ergonomic Problems Associated with Pipettes and Their Usage with Specific Reference to Work-Related Upper Limb Disorders. Applied Ergonomics, Vol. 28, No. 4: Hodgson E. (1996). Work Related Upper Limb Disorders and the Laboratory. World Directory of Environmental Testing, Monitoring and Treatment. Fredriksson K. (1995). Laboratory Work with Automatic Pipettes: A Study on How Pipetting Affects the Thumb, Ergonomics 38 (5): McGlothlin J.D., Hales T.R. (1995). NIOSH (National Institute of Occupational Safety and Health) Health Hazard Evaluation Report. Björkstén M.G., Almby B., Jansson E.S. (1994). Hand and Shoulder Ailments among Laboratory Technicians Using Modern Plunger-Operated Pipettes. Applied Ergonomics 25: Safety Mannonen, S., Riikonen, S. (2006) In Vitro Diagnostics (IVD) compliance and what it means in practice. Am. Biotech. Lab. 24(8), Mannonen, S. (2005) Use of a certified pipette tip to protect samples. Am. Biotech Lab. 23(8), Mannonen, S. (2005) Protecting your sample - Using a certified pipette tip. Int. Biotech. Lab. 23(3), Mannonen, S. (2005) Pipette tip cone filters minimise the risk of contamination. Labasia 12(4), 24-25; and Int. Labmate 30(5), Mannonen, S. and Hintikka, V. (2004). Poorly selected tips can cause major error to pipetting accuracy. Int. Labmate. 29(2), Mannonen S., Syrjä K. (2000). Safety in Pipetting. International Labmate, 25(1), 39. Mannonen S., Tiusanen T., Suovaniemi O. (2000). Major Sources of Error of Air Displacement Pipettes. Lab Asia, 7(2), Kolari M., Mannonen S., Takala T., Saris P., Suovaniemi O., Salkinoja-Salonen M.S. (1999). The Effect of Filters on Aseptic Pipetting Lifetime of Mechanical and Electronic Pipettes and Carryover during Pipetting. Letters in Applied Microbiology 29: Service & Maintenance Hemmings, Ian (2009). The importance of Regular In-Lab Checking of Pipettes. Mannonen S., Riikonen S. (2000). Accredited Calibration and Future Demands for Pipettes. International Biotechnology Laboratory, 18(2), Biohit Liquid Handling Products 91

92 Brochures Corporate Liquid Handling Diagnostics 92 Biohit Liquid Handling Products