CALYSTA Energy. Biological Gas-to-Liquids TM Biological Gas-to-Chemicals TM. Methane to Liquid Fuels and Chemicals. BIO Montreal - June /26/2013
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1 Biological Gas-to-Liquids Biological Gas-to-Chemicals Methane to Liquid Fuels and Chemicals BIO Montreal - June 2013 Copyright 2013 Calysta Energy 6/26/2013
2 Introduction to Calysta Biological conversion of methane using engineered organisms: Leverage proprietary biological tools & algorithms to rapidly enable bio-based conversion of methane Strong IP position through stealth mode operation Actively developing methane as the next-generation feedstock to improve current bio-processes 2011 Official Incorporation 2013 First commercial deal announced 2012 First public announcement Concept Developed DNA2.0 License & Financing
3 Thermodynamics and economics of carbon Banholzer, W. et al. Chem Eng Progress, March 2008, S7-S14 All products are downhill from methane Ethanol Ethylene Carbohydrate Key challenge is controlling the reaction, as full combustion is favored Methane is an advantaged feedstock relative to carbohydrates or CO 2 for making diesel or other reduced products Oil Carbon Monoxide Ethane CO2 + 2H2 Methane Coal Normalized Carbon Cost (US ct / MJ ) 3 Methane is one of the cheapest sources of high-energy carbon
4 Methanotrophs Bacteria that metabolize methane as their sole source of carbon and energy; generally obligate methane-users. Form complex chloroplast-like membrane structures for methane oxidation Only known class of organisms that can fix methane Tools for metabolic engineering and heterologous expression have not been available until now! 4
5 Metabolic Engineering in Methanotrophs Calysta has developed a set of novel engineering tools for methanotrophs Multiple promoters, markers, & plasmids cross-functional across species Efficient transformation methods, including electroporation Codon optimization, RBS tables, and other algorithms in progress Open-source approach to biology Tools are available to the research community at no cost 5
6 Advantaged approach to fermentation of chemicals & fuels Methane Calysta metabolically reengineered methanotrophs Fermentation Products Isoprene - Rubber - Plastics - Jet fuels Lactic Acid - PLA 2,3 Butanediol - Butadiene 4C Products - Crotonic acid - n-butanol Fatty Alcohols - Detergents Diesel Fuels Ethylene 6 Succinic acid
7 Pathway/organism interplay Sugar-based Operating System Metabolic Software Butanol Methane-based Operating System E. coli Hardware 3-HP Fatty Acids Methanotroph Hardware Sugar Isoprene Ethanol Methane Yeast Hardware Diols Lactate Operating system Developers Toolkit First-party software Metabolic Tools Calysta Products Succinate 7 Convert existing product pathways to use methane as a feedstock
8 Partnering to Move it Forward 8
9 Partnering to Move it Forward Multi-year R&D collaboration between 2 industry leaders Calysta: in feedstocks conversion using methanotrophic bacteria NatureWorks: in bioplastics, applications, and end-markets Exclusive partnership to research and develop a practical, world scale production process for fermenting methane into lactic acid Strategic fit: Calysta Perspective: Chemicals partnership a first step in goal of commercializing company s metabolic engineering assets, through licensing and partnerships with large producers of oil, gasoline, and chemicals. Biological Gas-to-Liquids Biological Gas-to-Chemicals For NatureWorks: Part of a much broader strategy of renewable feedstock diversification 9
10 From Methane to Plastics 1 It starts with Methane 2 Methanotrophs convert the gas into lactic acid through fermentation Methane is supplied directly to specialized bacteria called methanotrophs 3 NatureWorks converts lactic acid monomers to long polymer chains making a range of bioplastics 10
11 Calysta Energy: At the Right Place at the Right Time Biology provides a real opportunity to produce high-value chemicals from novel feedstocks Calysta is a first mover in bioconversion of methane, enabling preferred partners to dramatically reduce feedstock costs Best-in-class technology, experienced management team, solid financial backing, extensive intellectual property, proven business model, and the right set of tools and experience With first partnership in place, actively seeking additional partnerships to adapt validated pathways to methanotrophic hosts 11
12 Biological Gas-to-Liquids Biological Gas-to-Chemicals Methane to Liquid Fuels and Chemicals BIO Montreal - June 2013 Copyright 2013 Calysta Energy 6/26/2013
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