RIGOL. Service Guide. L-3200 Series Pump. Nov RIGOL Technologies, Inc.

Transcription

1 RIGOL Service Guide L-3200 Series Pump Nov RIGOL Technologies, Inc.

2

3 RIGOL Guaranty and Declaration Copyright 2012 RIGOL Technologies, Inc. All Rights Reserved. Trademark Information RIGOL is a registered trademark of RIGOL Technologies, Inc. Notices RIGOL products are protected by patent law in and outside of P.R.C. RIGOL reserves the right to modify or change parts of or all the specifications and pricing policies at company s sole decision. Information in this publication replaces all previously corresponding material. RIGOL shall not be liable for losses caused by either incidental or consequential in connection with the furnishing, use or performance of this manual as well as any information contained. Any part of this document is forbidden to be copied or photocopied or rearranged without prior written approval of RIGOL. Product Certification RIGOL guarantees this product conforms to the national and industrial standards in China as well as the ISO9001:2008 standard and the ISO14001:2004 standard. Other international standard conformance certification is in progress. Beijing No Contact Us If you have any problem or requirement when using our products, please contact RIGOL Technologies, Inc. or your local distributors, or visit: L-3200 Series Pump Service Guide I

4 RIGOL Safety Requirement General Safety Summary Please review the following safety precautions carefully before putting the instrument into operation so as to avoid any personal injuries or damages to the instrument and any product connected to it. To prevent potential hazards, please use the instrument only specified by this manual. Use Proper Power Cord. Only the power cord designed for the instrument and authorized by local country could be used. Ground The Instrument. The instrument is grounded through the Protective Earth lead of the power cord. To avoid electric shock, it is essential to connect the earth terminal of power cord to the Protective Earth terminal before any inputs or outputs. Observe All Terminal Ratings. To avoid fire or shock hazard, observe all ratings and markers on the instrument and check your manual for more information about ratings before connecting. Do not Assemble/Disassemble the Instrument without Authorization The instrument should be assembled and or disassembled by RIGOL s field engineers, users are not allowed to perform related operations besides some possible replacements in this manual. Do not Move or Maintain the Instrument When Power On To avoid personnel injuries, any position adjustment, maintenance or parts replacement are forbidden when instrument is on. Do Not Operate with Suspected Failures If suspected damage occurs with the instrument, have it inspected by qualified service personnel before further operations. Keep Well Ventilation The instrument must be placed in a well ventilation position (avoid cross-ventilation) without an open flame as solvent used by it are mostly organic having certain volatility and inflammability. Please check the vent and fan regularly. Do Not Operate In an Explosive Atmosphere In order to avoid damages to the device or personal injuries, the device should be operated away from explosive atmosphere to avoid sharp pounding and electromagnetic interference. II L-3200 Series Pump Service Guide

5 RIGOL Electrostatic Prevention Sparks generated by electrostatic charges in large amount may fire organic solvent in high concentration or even cause fire, please take precautions against static electricity. Operate the Instrument in a Suitable Environment The best condition for optimal measurement is: 5 to 35 ; less than 2 /hour changing, 20% to 80% RH and the instrument should not be placed towards the air outlet. Proper Use of Solvent Please use the solvent in compliance with instructions specified in its supplier. If necessary, wear protective clothing, gloves and glasses to avoid any injuries. Handling Safety Please handle with care during transporting to avoid damages to surface, panel and parts resulting from the falling from your hand. L-3200 Series Pump Service Guide III

6 RIGOL Safety Terms and Symbols Terms in this Manual. These terms may appear in this manual: WARNING Warning statements indicate the conditions or practices that could result in injury or loss of life. CAUTION Caution statements indicate the conditions or practices that could result in damage to this product or other property. Terms on the Product. These terms may appear on the Product: DANGER WARNING CAUTION indicates an injury or hazard may immediately happen. indicates an injury or hazard may be accessible potentially. indicates a potential damage to the instrument or other property might occur. Symbols on the Product. These symbols may appear on the product: Hazardous Voltage Safety Warning Protective Earth Terminal Chassis Ground Test Ground IV L-3200 Series Pump Service Guide

7 RIGOL Overview This manual is used to guide users to quickly understand how to use and maintain L-3200 series pump. Before operating the instrument, you are recommended to read this manual thoroughly to get a full understanding of the instrument. Almost all descriptions in this manual apply to L-3210, L-3220 and L-3240 pumps. Therefore, the term the pump is used throughout the manual to replace L-3200 series pump. If some function or information is particular to a certain model or version of pump, the instrument model will be listed (for example, L-3210 or L-3210 pump). Note: The pump configuration or function may vary, so not all descriptions apply to your particular pump. All descriptions in this manual apply to L-3200 series pump unless otherwise specified. L-3200 Series Pump Service Guide V

8 RIGOL Content Guaranty and Declaration... I Safety Requirement... II General Safety Summary... II Safety Terms and Symbols... IV Overview... V Chapter 1 Pump Structure Rear Panel Power Connection Fluid Path Internal Structure Chapter 2 Component Structure Main Control Board Power Supply Board Step Motor Driver Chapter 3 Debug To Start Ocelot To Set/Inquire Pump Information Pressure Correction Flow Rate Correction Static Leak Tightness Test Dynamic Leak Tightness Test Leakage Alarm Test Chapter 4 Maintenance and Service To Remove the Top Cover To Replace the Fuse To Replacing the Exhaust Valve Knob Rod O-ring To Replace the Filter Bowl Component To Replace the Exhaust Valve Component To Replace the Inlet check Valve To Replace the Outlet check Valve To Replace the Pump Head Component To Remove the Pump Head and Plunger Rod To Replace the Seal To Install the Pump Head and Plunger Rod Replace the Step Motor To Replace the Leakage Sensor Component To Replace the Pulse Damper To Replace the Main Control Board To Replace the Power Supply Board To Replace the Gradient Valve (L-3240) VI L-3200 Series Pump Service Guide

9 RIGOL To Replace the Gradient Valve Pinboard (L-3240) Chapter 5 Troubleshooting Overview VFD Screen Information Troubleshooting Chapter 6 Firmware Update Chapter 7 Supplies and Accessories L-3200 Series Pump Service Guide VII

10

11 Chapter 1 Pump Structure RIGOL Chapter 1 Pump Structure The contents of this chapter are as follows: Rear Panel Power Connection Fluid Path Internal Structure Rear Panel The rear panel contains the power socket (including two fuses), axial fan (used to release the heat inside the chassis), interface board interface (option) and main control board interface (as shown in Figure 1-1). Figure 1-1 Rear Panel View Power Connection Use the power cord supplied by RIGOL to connect the instrument to the main power source. For minimum interference effects, all components of the L-3000 system should be connected to the same mains output. Fluid Path As shown in Figure 1-2 (take L-3220 for example), two different mobile phases are transported to pump A and pump B respectively after being degassed. The degassed mobile phases flow into the primary pump heads through the inlet check valve and L-3200 Series Pump Service Guide 1-1

12 RIGOL Chapter 1 Pump Structure then are transmitted into the secondary pump heads through the outlet check valve driven by the motor (which rotates the primary cam and enables the plungers to do reciprocating motions); When both pump heads complete the aspiration and drain, the mobile phase are transmitted into the pressure sensor which can detect system pressure, feedback pressure compensation information and execute pressure protection. The mobile phase then runs into the pulse damper which inhibits pressure pulsation. The mixing ratio of mobile phases can be adjusted by setting the flow rates of pump A and pump B. After that, the mixed mobile phase enters the exhaust valve by which air bubbles can be removes when it is opened. At last the mobile phase flows into the in-line filter, from which the possible particle matter can be removed from the mobile phase; then the filtered mobile phase is used as the carrier to transmit the sample from the sampler into the column. Mobile phase 1 Vacuum degasser Mobile phase 2 Pump A Primary pump Pump B Primary pump Secondary pump Secondary pump Three-way valve Pressure sensor Pulse damper Column Sampler Inline filter Exhaust valve Figure 1-2 L-3220 Fluid Path 1-2 L-3200 Series Pump Service Guide

13 Chapter 1 Pump Structure RIGOL Internal Structure Figure 1-3 clearly shows the internal structure of the pump (take L-3220 for example). Rear Panel Fan Main Board Control Power board supply Moto Driver Step motor Pulse Damper Pump Figure 1-3 L-3220 Pump Internal Structure (Top View) The front plate and tray CAN NOT contact organic solvents, such as methanol, acetonitrile, chloroform, methylene dichloride and tetrahydrofuran. If organic solvent is spilled onto the front panel or tray, please wipe it with dry cloth or filter paper. L-3200 Series Pump Service Guide 1-3

14

15 Chapter 2 Component Structure RIGOL Chapter 2 Component Structure The contents of this chapter are as follows: Main Control Board Power Supply Board Step Motor Driver Main Control Board For the structure of the main control board, please refer to Figure 2-1. Figure 2-1 Main Control Board Interface Explanation List: J1. Reserved J11. USB J2. Pressure Sensor J12. Clean the motor J3. Reserved J13. Leakage Detect J4. CAN Bus J14. Gradient Valve J5. Fan J15. Photoelectric Switch (Pump B) J6. Driver J16. Reserved L-3200 Series Pump Service Guide 2-1

16 RIGOL Chapter 2 Component Structure J7. Reserved J17. Photoelectric Switch (Pump A) J8. VFD Screen J18. Reserved J9. Reserved JP1. Main Control Board Power J10. RS232 JP2. JTAG Power Supply Board Main control board power Power, AC220V Figure 2-2 Power Supply Board Driver power 2-2 L-3200 Series Pump Service Guide

17 Chapter 2 Component Structure RIGOL Step Motor Driver Figure 2-3 Driver L-3200 Series Pump Service Guide 2-3

18

19 Chapter 3 Debug RIGOL Chapter 3 Debug The contents of this chapter are as follows: To Start Ocelot To Set/Inquire Pump Information Pressure Correction Flow Rate Correction Static Leak Tightness Test Dynamic Leak Tightness Test Leakage Alarm Test To Start Ocelot Operation steps: 1. Turn on the pump; connect the pump data interface and the serial interface of the PC using serial cable. 2. Start the ocelot debug software. Select COM1 or other proper port for 通讯端口 (communication port) and click 连接 (connect). (As shown in Figure 3-1). Figure 3-1 Pump Main Control Interface L-3200 Series Pump Service Guide 3-1

20 RIGOL Chapter 3 Debug To Set/Inquire Pump Information Operation steps: Click 泵参数 (pump parameter) in the 控制主界面 (main control interface) and the interface as shown in Figure 3-2 appears. Users can set and inquire the pump information, such as 泵型号 (pump model), 出厂日期 (data of production), 泵序列号 (pump serial number) and 结构版本号 (structure version number). If a new main control board is used, fill the 设置 - 硬件 (settings hardware), 设置 -Fram (settings- Fram), 设置序列号 (serial number setting), 结构版本 - 设置 (structure version-setting) and Flash 版本 - 设置 (Flash version-setting) with the new version number or serial number. Figure 3-2 Pump Information Setup and Inquire Interface 3-2 L-3200 Series Pump Service Guide

21 Chapter 3 Debug RIGOL Pressure Correction Operation steps: 1. Insert the inlet tubing of the pump into a bottle of ultrapure water, then open the exhaust valve (as shown in Figure 3-3). Set 流速 (flow rate) to 1mL/min in the 控制主界面 (main control interface). Click 初始流速 (initial flow rate) and the pump starts. Click 泵校正 (pump correction) and the 泵校正 (pump correction) dialog box (as shown in Figure 3-4) is displayed. 10 minutes after when the pump runs steady click 压力清零 (clear pressure). Figure 3-3 Open the Exhaust Valve Figure 3-4 Pump Correction Interface L-3200 Series Pump Service Guide 3-3

22 RIGOL Chapter 3 Debug 2. Click 停泵 (stop pump) in the 控制主界面 (main control interface) and connect the pressure meter to the outlet at the right of the exhaust valve (as shown in Figure 3-5). Figure 3-5 Connect the Pressure Meter 3. Set the pressure upper limit to 6000PSI and the flow rate to 0.3mL/min. Click 初始流速 (initial flow rate) and close the exhaust valve. 4. The 压力超限 / 停泵 (pressure overrun/stop pump dialog box (as shown in Figure 3-6)will be displayed when the pressure reaches the upper limit. Click 确定 (OK) and immediately input 6000 in 校正压力 (correction pressure)". Observe the pressure meter and click 压力校正 (pressure correction) when the pressure meter shows 6000PSI. Figure 3-6 Pressure Overrun/Stop Pump Dialog Box 5. Input 5000 in 校正压力 (correction pressure)". Observe the pressure meter and click 压力校正 (pressure correction) when the pressure meter shows 5000PSI. Keep on correct 4000psi, 3000psi and 2000psi in the same way. 6. When the pump pressure returns to 0, run the pump again and increase the pressure. When the pump stops, compare the pressure readings in Ocelot and pressure meter. If the difference is less than 10PSI, the instrument is up to standard; otherwise, correct the pressure again. 7. Click 查询压力校正斜率 (inquire pressure correction slope" in 泵校正 (pump correction) to get the pressure correction coefficient. Record and keep it. 3-4 L-3200 Series Pump Service Guide

23 Chapter 3 Debug RIGOL Flow Rate Correction Operation steps: Prepare the flow meter (or measuring cylinder) and PEEK tubing. Use the flow rate correction coefficient principle, use water as mobile phase and add back pressure on PEEK tubing (within 6Mpa (870PSI) and 10Mpa (1450PSI)). 1. Prepare well-degassed HPLC grade water; connect one end of the PEEK backpressure tubing to the pump outlet and the other end to the flow meter or measuring cylinder. 2. Check the solvent compression coefficient in the 泵校正 (pump correction) interface. If the 查询压缩系数 ( inquire compression coefficient) is not 46 (the compression coefficient of water), set it to 46. Figure 3-7 Pump Correction Interface 3. Check whether the 用户校正系数 (user correction coefficient) is 10. If not, set it to Set the flow rate to 1ml/min. Correct the flow rate when the pressure reading is stable. 5. Flow rate correction should be done after pressure pulsation rejection calibration. Set the flow rate to 1ml/min. Record the measured flow rates and calculate the average. Note that the correction coefficient hold 4 places after the decimal point. New Correction Coefficient=Setting Flow Rate/Measured Average Flow Rate*Old Correction Coefficient For example, set the flow rate to 1ml/min. If the average flow rate is L-3200 Series Pump Service Guide 3-5

24 RIGOL Chapter 3 Debug 0.983mL/min and the old correction coefficient is , the new correction coefficient=1/0.983 *1.0102= Input in 小流速校正系数 (small flow rate correction coefficient, click 小流速校正系数, then set a new flow rate and run the pump. 6. Repeat step 5 until the flow rate meets the test requirements. Flow rate output error: ±0.5%, Flow rate stability: 0.1%. 7. Validation: set the flow rate to 0.5 ml/min and click 初始流速 (initial flow rate). Observe and record the actual flow rate. The flow rate output error should be ±1% and the flow rate stability be 0.2%. 8. According to the above method, set the flow rate to 2 ml/min to correct the correction coefficient in 中流速校正系数 (middle flow rate correction coefficient), and set the flow rate to 3 ml/min to correct the correction coefficient in 大流速校正系数 (large flow rate correction coefficient). Calculate the corresponding calibration coefficient and set the parameter according to the formula. The flow rate output error should be ±1% and the flow rate stability be 0.1%. Test 6 times for every flow rate, and respectively use formula (1) and formula (2) to calculate the flow rate output error and flow rate stability. Fm Fs S S = 100% (1) Fs F max F min S R = 100% (2) F m In the equation: S S Flow rate output error %; S R Flow rate stability %; F m actual flow rate (ml/min); F m the average of 6 measurement values under the same set flow rate (ml/min); F S the set flow rate (ml/min); F max maximum value of 6 measurement values under the same set flow rate (ml/min); F min minimum value of 6 measurement values under the same set flow rate (ml/min). 3-6 L-3200 Series Pump Service Guide

25 Chapter 3 Debug RIGOL Static Leak Tightness Test Operation steps: 1. Seal the outlet at the right of the exhaust valve using plug when the pump is in stop state ( as shown in Figure 3-8). Figure 3-8 Seal the Outlet at the Right of the Exhaust Valve 2. Open the exhaust valve, use redistilled water as mobile phase, set the flow rate to 5ml/min and pump A to 100%. Click 初始流速 (initial flow rate) to remove bubbles. 3. When the bubbles are removed, stop the pump. Set the pressure upper limit to 7200PSI (8000PSI*90%) and the flow rate to 0.1ml/min. Close the exhaust valve and click 初始流速 (initial flow rate). The beeper sounds and the pump stops When the pressure reaches 7200PSI. 4. Click 压力采集界面 (pressure acquisition interface), and record the current pressure value. The total pressure drop shall not be more than 700PSI during 10min. The pressure drop speed should be less than 30PSI per minute during another 10min. 5. Set pump B to 100%, and repeat steps 2-4. Dynamic Leak Tightness Test Operation steps: 1. When the pump is in stop state, connect one end of the backpressure tubing (providing about 7200PSI back pressure) to the outlet at the right of the exhaust valve and the other end to the waste bottle. 2. Set the pressure upper limit to 8000PSI. Input 100 in A % when using single pump, and respectively input 50 in A % and B % when using double pumps. 3. Use redistilled water as Mobile phase and set a proper flow rate to make the L-3200 Series Pump Service Guide 3-7

26 RIGOL Chapter 3 Debug pressure rise to about 7200PSI. Run the pump for 0.5h. Check the joints and connecting tubings (as shown in Figure 3-9) using filter paper to confirm there is no leakage. Note: To achieve the highest pressure, the flow rate can be properly adjusted, but it cannot exceed 2 ml/min. Figure 3-9 Check the Connections for Leakage 3-8 L-3200 Series Pump Service Guide

27 Chapter 3 Debug RIGOL Leakage Alarm Test Operation steps: 1. Click 漏液灵敏度 (leakage sensitivity), and then click 保存当前的漏液检测基准 (save the current leakage detection reference) (as shown in Figure 3-10). Check whether the absolute value of the result of the formula displayed on the right is less than 10; if not, please use air gun to blow the leakage sensor for 1min. Click the button again to view the result until the value meets the requirement; otherwise, please replace the leakage sensor component, and repeat the steps above. Figure 3-10 Pump Leakage Temperature Limit 2. Click 查询 (inquire) to check whether the result shows 0.7; if not, set it to 0.7 in 设置 (set) and click 设置 (set). 3. Click 开关查询 (switch inquire) of 漏液 (leakage) in the 控制主界面 (main control interface) (as shown in Figure 3-11). If it shows 关闭 (OFF), select 打开 (ON) in 开关设置 (switch setting) and click 设置 (set). Figure 3-11 Main Control Interface 4. Drop 1ml water using the dropper near the leakage sensors on the leakage tray. After about 1 min, the LED lamp turns red and flashes. L-3200 Series Pump Service Guide 3-9

28 RIGOL Chapter 3 Debug 5. Blow the leakage sensor with high-pressure air gun and observe whether the LED lamp turns green within 1 min. If not, please replace the leakage sensor L-3200 Series Pump Service Guide

29 Chapter 4 Maintenance and Service RIGOL Chapter 4 Maintenance and Service The contents of this chapter are as follows: To Remove the Top Cover To Replace the Fuse To Replacing the Exhaust Valve Knob Rod O-ring To Replace the Filter Bowl Component To Replace the Exhaust Valve Component To Replace the Inlet check Valve To Replace the Outlet check Valve To Replace the Pump Head Component Replace the Step Motor To Replace the Leakage Sensor Component To Replace the Pulse Damper To Replace the Main Control Board To Replace the Power Supply Board To Replace the Gradient Valve (L-3240) To Replace the Gradient Valve Pinboard (L-3240) L-3200 Series Pump Service Guide 4-1

30 RIGOL Chapter 4 Maintenance and Service To Remove the Top Cover Tool Name Specification/Quantity Plum screwdriver T20/1 Note: After removing the screws, push the top cover backwards and then lift the top cover. Operation steps: 1. Turn off the pump and remove the power cord. 2. Remove the four screws (marked out in Figure 4-1) using a T20 plum screwdriver. Push the top cover in the direction of the arrow in Figure 4-2 to separate it from the front panel, and then lift the top cover carefully to avoid fracturing the front panel. Figure 4-1 Pump Back View 4-2 L-3200 Series Pump Service Guide

31 Chapter 4 Maintenance and Service RIGOL Figure 4-2 Remove the Pump Top Cover To Replace the Fuse Material Name Quantity 250V 3A, Slowly melting type, #5*20mm 1 Tool Name Specification/Quantity Straight screwdriver --/1 Note: Before replacing the fuse, make sure the pump is turned off and the power cord is removed to avoid electric shock. Only use original RIGOL fuses for replacement. Operation steps: 1. Turn off the pump and remove the power cord. Prize out the fuse seat using a straight screwdriver and pull out the fuse seat as shown in 错误! 未找到引用源. L-3200 Series Pump Service Guide 4-3

32 RIGOL Chapter 4 Maintenance and Service Figure 4-3 Take off the Fuse Seat 2. Take out the burned fuse and install the new fuse of the same specification to the fuse seat as shown in Figure 4-4. Figure 4-4 Install a New Fuse 4-4 L-3200 Series Pump Service Guide

33 Chapter 4 Maintenance and Service RIGOL 3. Reinstall the fuse seat, as shown in Figure 4-5. Figure 4-5 Install the Fuse Seat 4. Power on the instrument and if the instrument starts normally, the replacement is correct; otherwise, repeat the above steps. To Replacing the Exhaust Valve Knob Rod O-ring Material Name Quantity Exhaust valve knob rod O-ring 1 Tool Name Specification/Quantity Wrench 5/16in/1, 1/2in/1 Plum screwdriver T20/1 Rubber gloves --/1 Note: Tools can be used to remove the exhaust valve knob rod O-ring, but make sure the tools will not scratch the metal surface of the knob rod. L-3200 Series Pump Service Guide 4-5

34 RIGOL Chapter 4 Maintenance and Service Operation steps: 1. Turn off the pump and remove the power cord. 2. Remove all the mobile phase in the tubing. And disassemble the in-line filter tubing using 6/15in and 1/2in wrenches and 4mm inner hexagon wrench. 3. Unscrew the exhaust valve knob, as shown in Figure 4-6. Figure 4-6 Unscrew the Exhaust Valve Knob 4. Tools can be used to remove the O-ring, however, make sure the tools don t scratch the metal surface of the knob rod. Install the new O-ring, as shown in Figure 4-7. Place the O-ring on the exhaust knob and installed it to the appropriate location. 4-6 L-3200 Series Pump Service Guide

35 Chapter 4 Maintenance and Service RIGOL Figure 4-7 Install the O-ring 5. Install the exhaust valve knob and perform leak tightness test ( for details, refer to Dynamic Leak Tightness Test ). To Replace the Filter Bowl Component Material Name Quantity Filter bowl seal component 1 Tool Name Specification/Quantity Wrench 5/16in/1, 1/2in/1 Plum screwdriver T20/1 Rubber gloves --/1 Note: During installation, you must wear rubber gloves to avoid contamination. Operation steps: 1. Turn off the pump and remove the power cord. L-3200 Series Pump Service Guide 4-7

36 RIGOL Chapter 4 Maintenance and Service 2. Clear all the mobile phase in the tubing. 6/15in, 1/2in wrenches and 4mm inner hexagon wrench can be used to remove the in-line filter tubing. 3. Remove the in-line filter Component and take off the in-line filter sleeve, as shown in Figure 4-8. Figure 4-8 Take off the In-line Filter Sleeve 4. Replace the filter bowl, as shown in Figure 4-9. Put a new filter bowl into the filter sleeve and fix the filter bowl with filter bowl seal. Note that rubber gloves must be worn during operation to prevent pollution of the Fluid path. 4-8 L-3200 Series Pump Service Guide

37 Chapter 4 Maintenance and Service RIGOL Figure 4-9 Replace the Filter Bowl L-3200 Series Pump Service Guide 4-9

38 RIGOL Chapter 4 Maintenance and Service 5. Reinstall the filter sleeve and the tubing. 6. Test the inline filter: Remove the other components at the solvent outlet and prepare the mobile phase. Switch on the instrument and run RIGOL CWS. Set the flow rate to 1ml/min and run the pump. If the displayed pressure is 0.3Mpa, the replacement is successful. To Replace the Exhaust Valve Component Material Name Quantity Exhaust Valve Component 1 Tool Name Specification/Quantity Wrench 5/16in/1, 1/2in/1 Plum screwdriver T20/1 Rubber gloves --/1 Note: Pay attention to the direction during installation; the outlet filter component should be on the right side. Avoid damaging the tubing when removing it so the tubing can be reused. Operation steps: 1. Turn off the pump and remove the power cord. 2. Remove all the mobile phase in the tubing. Take off the top cover. Remove the pressure sensor cable socket on the main control board (for details, refer to Main Control Board ) and remove the tubings on the exhaust valve one by one using 5/16in wrench (marked out in Figure 4-10) L-3200 Series Pump Service Guide

39 Chapter 4 Maintenance and Service RIGOL Figure 4-10 Tubings Connected to the Exhaust Valve 3. Remove the screws (the positions are as shown in Figure 4-11) connecting the exhaust valve component and chassis at the inner side of the chassis using T20 plum screwdriver. Remove the Exhaust Valve Component horizontally. Figure 4-11 Remove the Exhaust Valve Component 4. Push the new component into the exhaust valve hole on the chassis horizontally. Pay attention to the direction and the outlet filter component should be on the right side as shown in Figure Install the screws and insert the pressure sensor cable to the corresponding position on the main control board. L-3200 Series Pump Service Guide 4-11

40 RIGOL Chapter 4 Maintenance and Service The Outlet Filter component Figure 4-12 Install the Exhaust Valve Component 5. Connect the exhaust valve tubing. 6. Perform leak tightness test and pressure correction (for details, refer to Dynamic Leak Tightness Test and Pressure Correction ). To Replace the Inlet check Valve Material Name Quantity Inlet check valve 2 Inlet check valve nut: unef 1/ Tool Name Specification/Quantity Wrench 5/16in/1, 1/2in/1 Inner hexagon wrench 4mm/1 Rubber gloves --/1 Notes: Pay attention to the direction of the component during disassemble and assemble. Operation steps: 1. Turn off the pump and remove the power cord. 2. Clear all the mobile phases in the pump tubing and remove the tubing connected to the check valves (as marked out in Figure 4-13) using 5/16in, 1/2in wrench L-3200 Series Pump Service Guide

41 Chapter 4 Maintenance and Service RIGOL Figure 4-13 Tubings to the Check Valves 3. Remove the nuts fixing the inlet or outlet check valve using 1/2in wrench and take out the check valve. 4. Insert the new inlet into the nut (as shown in Figure 4-14) and pay attention to the directions. Note: The inlet check valve has letter I under the arrow (as shown in Figure 4-15). Figure 4-14 Install the Inlet Check Valve Figure 4-15 Inlet Check Valve 5. Reinstall the tubing and prepare the mobile phase. 6. Power on the pump and start the RIGOL CWS. 7. Set the flow rate to ml/min and start the pump. If solvent constantly flows L-3200 Series Pump Service Guide 4-13

42 RIGOL Chapter 4 Maintenance and Service out of the drain tubing, the replacement completes. 8. Perform the Dynamic Leak Tightness Test as described in Chapter Washing check valves. The check valves may fail due to contamination and it need be washed, the operation step list: Take out the check valves. Put the valves into the water, bath them for 20min in Ultrasonic washer. Replace the water with isopropanol then bath them for 20min in Ultrasonic washer. Put the valves back in pure water and bath them again in Ultrasonic washer. Then take them out, clean the solvent. To Replace the Outlet check Valve Material Name Quantity Outlet check valve 2 Outlet check valve nut: 7/ Tool Name Specification/Quantity Wrench 5/16in/1, 1/2in/1 Inner hexagon wrench 4mm/1 Rubber gloves --/1 Notes: Pay attention to the direction of the component during disassemble and assemble. Operation steps: 1. Turn off the pump and remove the power cord. 2. Clear all the mobile phases in the pump tubing and remove the tubing connected to the check valves (as marked out in Figure 4-13) using 5/16in, 1/2in wrench. Figure 4-16 Tubings to the Check Valves 4-14 L-3200 Series Pump Service Guide

43 Chapter 4 Maintenance and Service RIGOL 3. Remove the nut fixing the outlet check valve using 1/2in wrench and take out the check valve. 4. Insert the new outlet check valve into the nut (as shown in Figure 4-14) and pay attention to the directions. Note: The outlet check valve has letter O under the arrow (as shown in Figure Figure 4-17 Install the Outlet Check Valve Figure 4-18 Outlet Check Valve 5. Reinstall the tubing and prepare the mobile phase. 6. Power on the pump and start the RIGOL CWS. 7. Set the flow rate to ml/min and start the pump. If solvent constantly flows out of the drain tubing, the replacement completes. 8. Perform the Dynamic Leak Tightness Test as described in Chapter Washing check valves. The check valves may fail due to contamination and it need be washed, the operation step list: Take out the check valves. Put the valves into the water, bath them for 20min in Ultrasonic washer. Replace the water with isopropanol then bath them for 20min in Ultrasonic washer. Put the valves back in pure water and bath them again in Ultrasonic washer. Then take them out, clean the solvent. L-3200 Series Pump Service Guide 4-15

44 RIGOL Chapter 4 Maintenance and Service To Replace the Pump Head Component Material Name Quantity Pump head O-ring 1 High-pressure seal 1 High-pressure seal spacer 1 Cleaning seal 1 Cleaning seal spacer 1 1/4-28 low-pressure hollow 2 headless screw Cleaning FEP tubing 1 Tool Name Specification/Quantity Wrench 5/16in/1, 1/2in/1 Plum screwdriver T20/1 Rubber gloves --/1 Straight screwdriver --/1 Tweezers, sealing --/1 membrane To Remove the Pump Head and Plunger Rod Note: 1. Tubings can be easily damaged during disassemble and it costs lots of time to re-make the tubings, so be careful when you remove the tubings and remove the tubings according to the correct order. 2. Remove the pump head gently in horizontal direction to avoid damaging the plunger rod. Before removing and installing the plunger rod, insert a straight screwdriver or other appropriate tool between the piston and piston sleeve for easier removal and installation of the plunger rod and to avoid damaging the plunger rod 3. The pump head should be cleaned with ultrasonic in methanol. The two pump head screws should be tightened gradually and alternately, please don t tighten one screw first. 4. Rubber gloves are needed during the installation to avoid contamination L-3200 Series Pump Service Guide

45 Chapter 4 Maintenance and Service RIGOL Operation steps: 1. Turn off the pump and remove the power cord. 2. Clear all the mobile phase in the pump tubings and take off the top cover. Remove the tubings using 5/16in and 1/2in wrenches (as shown in Figure 4-19). Figure 4-19 Tubings Connecting to the Pump Head 3. Rotate the belt wheel using your hands to move the corresponding piston to the foremost side. Prize the spring using a screwdriver to separate the plunger rod and the piston sleeve and insert a hexagon spanner larger than 3mm into the opening (as shown in Figure 4-). Figure 4-20Insert a Tool between the Piston and Piston Sleeve 4. Remove the screws on the pump head using 4mm inner hexagon wrench as shown in Figure 4-; remove the pump head under the direction of the red arrow. L-3200 Series Pump Service Guide 4-17

46 RIGOL Chapter 4 Maintenance and Service Figure 4-21 Remove the Pump Head 5. Take out the plunger rod from the piston slot in horizontal direction as shown in Figure 4-. Figure 4-22 Take out the Plunger Rod 4-18 L-3200 Series Pump Service Guide

47 Chapter 4 Maintenance and Service RIGOL To Replace the Seal Note: 1. Put the high pressure seal, high pressure seal spacer, pump O-ring, cleaning seal and cleaning seal spacer in methanol. 2. Wrap the tweezers with sealing membrane to avoid scratching the pump head and seal. Rubber gloves are required during the operations. 3. Replace the high pressure seal spacer while replacing the high pressure seal. Operation steps: 1. Pull the plunger rod out from the cleaning head gently and take off the cleaning seal spacer. Twist the plunger rod into the high pressure seal to prize out the high-pressure seal as shown in Figure Note: don t let the plunger touch the pump head to avoid damaging the plunger rod. Figure 4-20 Take out the High-pressure Seal 2. Take out the cleaning seal from the cleaning head in the same way and take out the pump head O-ring using tweezers wrapped with sealing membrane from the seal as shown in Figure Figure 4-21 Take out the Pump Head O-ring L-3200 Series Pump Service Guide 4-19

48 RIGOL Chapter 4 Maintenance and Service 3. Take out the high-pressure seal, high pressure seal spacer, pump head O-ring and cleaning seal, cleaning seal spacer using tweezers wrapped with sealing membrane from the pump head and cleaning head. The high-pressure seals and high pressure seal spacer can t be reused. Put the cleaning seal, cleaning seal spacer, new high-pressure seal, high-pressure seal spacer and pump head O-ring in methanol (as shown in Figure 4-225). Figure Wash the Seal and Spacer 4. Put the cleaning head on the table with the big hole side facing upwards. Put the cleaning seal into the middle hole of the cleaning head with the side with black lines facing upwards and fix the cleaning seal into the hole using the cleaning seal spacer, as shown in Figure Figure Install the Cleaning Seal 4-20 L-3200 Series Pump Service Guide

49 Chapter 4 Maintenance and Service RIGOL 5. Cut the cleaning tubing using pipe cutter to keep the tubing end flat and pass it through the hollow screw and put on the compression ring. Screw the hollow screw into the cleaning head and during this process the cleaning tubing should be compressed; otherwise, leakage might occur, as shown in Figure 4-247; Figure Install the Cleaning Tubing 6. Cut the cleaning tubing at the position 1 cm away from the hollow screw head. For the other end of the cleaning tubing, repeat the above operations as shown in Figure Figure Cut the Cleaning Tubing 7. Rinse the plunger rod with methanol and clean the foreign matter on the plunger rod using filter paper, as shown in Figure Figure Rinse the Plunger Rod 8. Place the cleaned pump head on the table. Wet the seal hole on the pump head using methanol. Put the new high-pressure seal into the hole, and then the new L-3200 Series Pump Service Guide 4-21

50 RIGOL Chapter 4 Maintenance and Service high-pressure seal spacer with the groove facing upwards, finally press the seal with cleaning head to install in place (as shown in Figure 4-), then install it to the instrument. Figure 4-30 Install the Plunger Rod into the Pump Head 9. Connect a back pressure tube (which can provide psi pressure) to the outlet of the exhaust valve, set the pump to 2mL/min, clear the bubbles in the system then run the pump for 30min. 10. Perform static leak tightness test (for details, refer to Static Leak Tightness Test ). 11. Perform dynamic leak tightness test (for details, refer to Dynamic Leak Tightness Test ). 12. The seal lifespan counter should be reseted when a new seal has been installed. Open the Diagnosis & Maintenance of pump in workstation, click the Replace below the proper pump, and then click Yes to confirm the operation. To Install the Pump Head and Plunger Rod Note: Do not touch the plunger rod during the whole process. Operation steps: 1. Pull the two 1/16 joints on the cleaning head to take off the cleaning head gently. Hold the cleaning head and insert the plunger rod into the piston sleeve; then, pull the cleaning head into the pump head seat thoroughly with the cleaning seal spacer fitting into the round groove on the pump head seat (as shown in Figure 4-). Note that do not touch the plunger rod L-3200 Series Pump Service Guide

51 Chapter 4 Maintenance and Service RIGOL Figure 4-31 Install the Plunger Rod 2. Align the pump head with the cleaning head and plunger rod and push the pump head slowly until it touches the pump head seat. Pay attention to the installation direction (the RIGOL label should face upwards as shown in Figure 4-). When pushing the pump head, your strength should be well-distributed. L-3200 Series Pump Service Guide 4-23

52 RIGOL Chapter 4 Maintenance and Service Figure 4-32 Install the Pump Head 3. Press the top of the pump head with one hand. Install the pump head screws using 4mm inner hexagon wrench. Screw the two screws alternately. Don t tighten one screw first and then tighten the other one (as shown in Figure 4-27). Don t touch the plunger rod during the whole process. Figure 4-27 Install the Pump Head Screws 4. After the pump head installation is completed, remove the screwdriver or hex spanner between the springs. During this process, use a straight screw driver to make the plunger rod and piston sleeve touch each other slowly. Don t pull the screwdriver or hex spanner directly L-3200 Series Pump Service Guide

53 Chapter 4 Maintenance and Service RIGOL Replace the Step Motor Material Name Quantity Step motor 1 Tool Name Specification/Quantity Wrench 5/16in/1, 1/2in/1 Plum screwdriver T20/1 Striaght screwdriver --/1 Inner Hexagon wrench 4mm/1 Rubber gloves --/1 Note: The screws at the bottom of the pump chassis are elastic connections and you just need to screw them to proper positions. Operation steps: 1. Turn off the pump and remove the power cord. 2. Clear all the mobile phase in the tubings and remove the top cover (for details, refer to To Remove the Top Cover ), pump head tubings, pump head and plunger rod (for details, refer to To Remove the Pump Head and Plunger Rod ) as well as the pump head seat. Remove the four screws at the bottom of the pump chassis using 4mm inner hexagon wrench. The screws positions are as shown in Figure Remove the pump component. Figure 4-28 Positions of the Screws at the Bottom of the Pump Chassis 3. Remove the four screws that attach the step motor using 4mm inner hexagon wrench and take off the step motor, as shown in Figure L-3200 Series Pump Service Guide 4-25

54 RIGOL Chapter 4 Maintenance and Service Figure 4-29 Take off the Step Motor 4. Install the new step motor; install the pump into the chassis; install the pump head ; connect the tubings and run the pump. 5. Perform leak tightness test (for details, refer to Dynamic Leak Tightness Test ) L-3200 Series Pump Service Guide

55 Chapter 4 Maintenance and Service RIGOL To Replace the Leakage Sensor Component Material Name Quantity Leakage sensor component 1 Tool Name Specification/Quantity Plum screwdriver T20/1 Operation steps: 1. Turn off the pump and remove the power cord. 2. Disconnect the leakage sensor cable from the main control board (for details, refer to Main Control Board ). Remove the screw connecting the leakage sensor component and the pump chassis. Take off the leakage sensor component and draw its cable out from the cable hole, as shown in Figure Figure 4-30 Remove the Leakage Sensor Component Cable 3. Pass the cable of the new leakage sensor component through the cable hole on the pump chassis. Connect the leakage sensor Component to the chassis using T20 torx pan head screws and insert the cable to the corresponding position on the main control board (refer to Main Control Board ). L-3200 Series Pump Service Guide 4-27

56 RIGOL Chapter 4 Maintenance and Service 4. Correct the sensitivity of the Leakage sensor (for the leakage sensor Component test, refer to Leakage Alarm Test ). To Replace the Pulse Damper Material Name Quantity Pamper 1 Tool Name Specification/Quantity Wrench 1/2in/ 1 Plum screwdriver T20/1 Inner hexagon wrench 4mm/ 1 Operation steps: 1. Turn off the pump and remove the power cord. 2. Clear all the mobile phase in the tubings and remove the tubings connecting to the pulse damper using 1/2in wrench. 3. Remove the four screws connecting the pulse damper and chassis using T10 plum screwdriver and Phillips screwdriver, as shown in Figure Figure 4-31 Screws Connecting the Pulse Damper and Chassis 4. Remove the tubings connecting the pulse damper and the four screws fixing the pulse damper stand and seat using 1/2in wrench and T20 plum screwdriver(as shown in Figure 4-38). Be careful when removing the tubings to avoid damaging them L-3200 Series Pump Service Guide

57 Chapter 4 Maintenance and Service RIGOL Figure 4-32 Remove the Tubings Connecting the Pulse Damper 5. Remove the pulse damper cover and take out the pulse damper and the stand from the chassis, as shown in Figure Figure 4-33 Take out the Pulse Damper and Stand L-3200 Series Pump Service Guide 4-29

58 RIGOL Chapter 4 Maintenance and Service 6. Remove the six screws connecting the fixing hoop and pulse damper stand using T20 plum screwdriver (or inner hexagon wrench) and take off the fixing hoop. Remove the tubings connecting the pulse damper using the wrench, as shown in Figure 4-. Figure 4-40 Remove the Tubings Connecting the Pulse Damper 7. Replace the pulse damper. Connect the original tubings to the new pulse damper if they are not damaged during disassemble; otherwise, use new tubings. Install the pulse damper to the chassis according to the correct order and connect the system tubings. 8. Perform leak tightness test (for details, refer to Dynamic Leak Tightness Test) L-3200 Series Pump Service Guide

59 Chapter 4 Maintenance and Service RIGOL To Replace the Main Control Board Material Name Quantity Main control board 1 Tool Name Specification/Quantity Wrench 5/16in/1, 1/2in/1 Plum screwdriver T20/1 T10/1 Operation steps: 1. Turn off the pump and remove the power cord. 2. Remove the top cover. Disconnect all cable connections from the main control board. Remove the three torx pan head screws connecting the main control board and chassis, as shown in Figure 4-. Figure 4-41 Screws Connecting the Main Control Board and Chassis 3. Remove the four screws fixing the main control board using T10 plum screwdriver. 4. Install the new main control board and screw the screws. Insert the cables to L-3200 Series Pump Service Guide 4-31

60 RIGOL Chapter 4 Maintenance and Service the corresponding positions on the main control board according to the main control board cable chart (for details, refer to Main Control Board ). 5. Reinstall the top cover. Connect the power cord and turn on the pump. 6. Set the pump parameters and perform pressure calibration, pressure pulse rejection correction and flow rate correction (for details, refer to To Set/Inquire Pump Information, Pressure Correction, 错误! 未找到引用源 and Flow Rate Correction ). To Replace the Power Supply Board Material Name Quantity Power supply board 1 Component Tool Name Specification/Quantity Wrench 5/16in/1, 1/2in/1 Plum screwdriver T20/1 Operation steps: 1. Turn off the pump and remove the power cord. 2. Remove the top cover. Remove the four screws (as shown in Figure 4-) connecting the power supply board and chassis using T10 plum screwdriver. Take out the power supply board Component. Figure 4-42 Screws Connecting the Power SupplyBoard and Chassis 3. Install the new or repaired power supply board. Install the power supply board into the chassis, pay attention to the Power supply board direction and align the four screws with the four bolts on the chassis. Insert the cables. Install the power supply cover and screw the screws, as shown in Figure L-3200 Series Pump Service Guide

61 Chapter 4 Maintenance and Service RIGOL Figure 4-34 Install the New Power Supply Board 4. Install the top cover and connect the power cord. Turn on the pump. To Replace the Gradient Valve (L-3240) Material Name Quantity Quaternary Gradient valve 1 Tool Name Specification/Quantity Plum screwdriver T10/1 Operation steps: 1. Turn off the pump and remove the power cord. 2. Clear all the mobile phase in the tubings. Disconnect all tubing connections from the quaternary gradient valve. 3. Remove the top cover. Remove all cables from the quaternary gradient valve. Remove the two screws (as shown in Figure 4-35) connecting the gradient valve and chassis using T10 plum screwdriver. L-3200 Series Pump Service Guide 4-33

62 RIGOL Chapter 4 Maintenance and Service Figure 4-35 Screws Connecting the Quaternary Gradient and Chassis 4. Install the new quaternary gradient valve and insert the cables to the corresponding positions on the gradient valve pinboard as shown in Figure Figure 4-36 Install the New Gradient Valve 5. Install the top cover and connect the tubings to the gradient valve. Connect the power cord and turn on the pump. Set the gradient method and check whether the gradient valve can work normally. To Replace the Gradient Valve Pinboard (L-3240) Material Name Quantity Gradient valve pinboard 1 Tool Name Specification/Quantity Plum screwdriver T10/1 Operation steps: 1. Turn off the pump and remove the power cord. 2. Remove the top cover. Remove all cables from the gradient valve pinboard. Remove the four screws (as shown in Figure 4-37) connecting the gradient valve 4-34 L-3200 Series Pump Service Guide

63 Chapter 4 Maintenance and Service RIGOL pinboard and gradient valve stand. Figure 4-37 Screws Connecting the Gradient Valve Pinboard and Stand 3. Install the new gradient valve pinboard, screw the screws and insert all the cables to the corresponding positions on the gradient valve pinboard. 4. Reinstall the top cover and connect the tubings to the gradient valve. Connect the power cord. And turn on the pump. Set the gradient method to check whether the gradient valve can work normally. L-3200 Series Pump Service Guide 4-35

64

65 Chapter 5 Troubleshooting RIGOL Chapter 5 Troubleshooting The contents of this chapter are as follows: Overview VFD Screen Information Troubleshooting Overview With Ocelot, users can set and inquire pump information as well as perform system pressure correction, pressure clear and flow rate correction. When updating pump DSP, Ocelot can realize pressure pulse rejection calibration. When the pump is running into error, the corresponding information will be displayed on the VFD screen to help users to locate the failure type. If a commonly encountered failure (for details, refer to Troubleshooting ) occurs, users can dispose it according to this manual; if the problem remains, please contact RIGOL technical support. VFD Screen Information The pump statuses include: Status Description READY Ready for operation. Switch to this state after the instrument test the photoelectric switch in power-on self-test RUNNING Gradient table is running STANDBY Set the standby state. Stay in this state after the pump switches to the standby flow rate and before it switches to IDLE state IDLE Switch to this state when the gradient table runs to the last line or when runs the first line or the last line STOP Shutdown Display for 3 seconds after start-up WELCOME TO USE RIGOL L-3000 Display the module, name and program version for 3 seconds Component model and software version Component name L-3200 Series Pump Service Guide 5-1

66 RIGOL Chapter 5 Troubleshooting For example, Self-test information L-3220 *.** BINARY PUMP ISOCRATIC PUMP QUATERNARY PUMP Self-test Self-test status. For example, SELFCHECKING PLEASEWAIT. The pump self-test mainly check the photoelectric switch positions of the two step motors HOMEPOSITION HOMING PUMPA HOMING PUMOB ERROR: A-HOMEPOS Main page: display for 2s The current flow rate and the current pressure Current status. For example, 1.200ml 10000PSI REDAY. For gradient pump, the proportion interface will also be displayed: display for 2s (for double pump, only A and B will be displayed) A: 30.5 B: 30.5 C: 30.5 D: 8.5 Pressure upper and lower limits : display for 1s MAX: 6000PSI MIN: 100PSI Status information: Normal running state: READY. IDLERUNNING>>> STOPPED 5-2 L-3200 Series Pump Service Guide

67 Chapter 5 Troubleshooting RIGOL When the pump is running, > means the pump is pumping solvents in every 0.5 s. For example, PUMPING> PUMPING>> PUMPING>>> PUMPING> PUMPING>> PUMPING>>> There are four error states: ERROR: P-MAX ERROR: P-MIN ERROR: LEAK ERROR: HOMEPOS Troubleshooting This manual provides the possible reasons and solutions for failure information displayed on the VFD screen. It also provides the failures that might occur when the pump is running as well as their reasons and solutions. VFD Alarm Problem Possible Reason Solution The flow path is blocked Check whether the sinker filter and in-line filter is blocked, whether the check valve works normally and whether the Error: P-MAX other parts of the fluid path are The pressure blocked. exceeds the Pressure sensor error Check the pressure sensor upper limit The flow rate is too large Reduce the flow rate to normal level The maximum pressure is too low Set the maximum pressure limit to 6000PSI Leakage occurs Check for leakage, reduce the ERROR: P-MIN flow rate The pressure Pressure sensor error Check the pressure sensor exceeds the The flow rate is too small Reset the flow rate lower limit The minimum pressure is Set the minimum pressure limit too high for 100PSI Loose connection Reconnect the tubing ERROR: LEAK Plunger rod cleaning tubing Reconnect the cleaning tubing Leakage occurs leakage ERROR: HOME Photoelectric switch of Replace the photoelectric L-3200 Series Pump Service Guide 5-3

68 RIGOL Chapter 5 Troubleshooting POS Pump home position error SELFCHECKING ERROR Self-test error The VFD screen does not light home position error Driver error Power supply board error Main control panel error Motor error Power input error VFD screen error switch Check and replace the driver Check and replace the power supply board Check and replace the main control board Check and replace the step motor Reconnect the power Check and replace the VFD screen Operation Failure Problem Possible Reason Solution Loose connection Re-plug out/in the mains connection Faults in the power cable Replace a same cable The pump does Substandard power supply Use the specified power supply not start when Fuse was burn out Replace a same fuse power switch is Re-plug out/in the mains pressed Loose connection connection Other circuit part failure Check the circuit parts one by one Communication failure Restart the software and between the pump and PC re-communicate Pump flow rate is set to 0 Specify a non-zero rate The pump is not Pressure over range (upper running after limit) Reset the pressure upper limit clicking Run Communication failure Restart the software and between the pump and PC re-communicate Instrument configuration error Re-configure the instrument No bottle is placed at the fluid inlet or the intake Check the fluid inlet and intake tubing is not lowered into tubing the fluid No solvent in the flow path when the pump is running The instrument is not connected Air bubbles are found in the pump head The Check valve isn't working as it should The signal cable is abnormal Remove the air bubbles by the PURGE function Remove the valve and clean it by ultrasonic or replace a new one when necessary Reconnect the signal cable 5-4 L-3200 Series Pump Service Guide

69 Chapter 5 Troubleshooting RIGOL Unstable liquid transmission and bigger pressure pulsation The actual flow rate is lower then the setting value The pump DSP version is too low Air bubbles are found in the pump head There are bubbles in the system The plunger rod is not in the appropriate position for normal operation The Check valve isn't working properly The solvent is not degassed sufficiently The solvent is beyond its expiry date Last mobile phase existed in the pump head The in-line filter is blocked Pump head leakage Pulse damper failure The check valve is not working properly System leakage The flow rate is not accurate The check valve is not working properly Update the DSP Clean the sinker filter using ultrasonic and remove the air bubbles by using PURGE function Remove the bubbles Reinstall the plunger rod Remove the valve and clean it by ultrasonic or replace a new one when necessary Degas the solvent Use the fresh and filtered solvent; bacteria might generate in water Replace the last mobile phase according to the PURGE function Wash or replace the in-line filter Replace a new seal or plunger Replace the pulse damper Remove the check valve and clean it using ultrasonic or replace a new one Check if there is any leakage in the pump head or in some connection Recalibrate the flow rate Remove the check valve and clean it using ultrasonic or replace a new one See bigger pressure pulsation Re-configure the mobile phase Pressure fluctuation The retention Errors in mobile phase time is not stable settings The environment conditions (such as the temperature) are unstable The sample is degraded Remake the sample The pressure The purge valve is open Close the purge valve Keep stable environment conditions L-3200 Series Pump Service Guide 5-5

70 RIGOL Chapter 5 Troubleshooting doesn t rise to the expected value The pressure rises too fast and beyond the normal value Large baseline drift High noise level System leakage Check if there is any leakage in the pump head or in some connection Add the back pressure No back pressure (Column) is added Pressure sensor error Replace the pressure sensor The flow rate is too large Set a proper flow rate System tubing or part is Check the tubings and rinse or blocked replace the blocked part The column is blocked Rinse or replace the column The in-line filter is blocked Rinse or replace the in-line filter The column is Clean or replace the column contaminated The system stability time is Increase the system stability not enough time The solvent is beyond its Use fresh and filtered solvent; expiry date bacteria might generate in water The environmental Make sure that the conditions are unstable temperature and the humidity are constant; avoid fluctuation The detector is not stable wait for the detector to become stable The solvent is reused Dispose the old solvent; use fresh and filtered solvents The flow cell is dirty Clean the flow cell; replace the flow cell if necessary The lamp energy is not Replace the lamp enough There are bigger pressure Purge the pump fluctuations There are air bubbles in the Purge the pump system The solvent is beyond its Use the fresh and filtered expiry date solvents Detector failure See Detector Service Guide The lamp energy is not Replace the lamp enough Detection parameters error Reset the detection parameters When acetonitrile is used, Store the system use methanol the check valve may be (or use ceramic check valve blocked instead of sapphire check valve) Pulse damper (mixer) Replace the pulse damper 5-6 L-3200 Series Pump Service Guide

71 Chapter 5 Troubleshooting RIGOL Peak broadens and dead time increases Additional peaks other than the solvent peaks appear in the dead time failure The inner diameter of tubings is too large The in-line filter is blocked The tubings are blocked The sample loop is blocked The column is overloaded or contaminated The solvent is beyond its expiry date With gradients, the equilibration time after the flush cycle is too short or the dead volume is too large There are bubbles in the flow path The sampling speed for the sample is too high The sample contains lots of bubbles The needle is blocked or the needle tip is deformed Six-way valve or injection valve leakage (mixer) Use tubings with proper inner diameter Replace the in-line filter Replace the relevant tubings Replace the sample loop Reduce the injection volume, rinse or replace the column Use fresh solvent Increase the equilibration time or eliminate possibly dead volume Purge the whole system using thoroughly degassed liquid Reduce the sampling speed Degas the sample Replace the needle Tighten the corresponding part or replace the part Poor reproducibility Sample overload Flush the injection valve and adjust the inject volume Loose tubing connections Tighten the tubing connections or replace the joints There are dead volumes in the tubing connections. Replace the joints and install the joints and seating ring Plunger rod seal leakage Replace the plunger rod seal There is pump pressure pulsation Purge the pump and check pump tubing connections The gradient Change the gradient reproducibility is poor conditions; Check the degasser; Check the in-line filter and rinse or replace it; (Check the quaternary gradient valve) The sample is unstable and Use new sample degraded The environmental Make sure that the L-3200 Series Pump Service Guide 5-7

72 RIGOL Chapter 5 Troubleshooting No flow Large fluctuation Poor degassing flow Ghost peaks appear repeatedly in the chromatogram conditions are unstable Contamination occurs somewhere in the system The system is leaking The check valve is not installed correctly or are defective There are air bubbles in the pump head or solvent The in-line filter is blocked The plunger rod is not in the appropriate position for normal operation The inlet path is blocked There are bubbles in the inlet path The check valves is not installed correctly or defective The plunger rod connection is loose Tubing leakage The flow rate is too large The degassing channels are contaminated. The solvents are degraded or their purity is insufficient Contamination occurs somewhere in the system temperature and the humidity are constant; avoid fluctuation Flush the system use appropriate solvent Find and eliminate the leakage Rinse the check valve with ultrasonic and reinstall or replace it Purge the pump Replace the in-line filter Reinstall the plunger rod Check the inlet lines, sinker filter (quaternary gradient valve) of the pump etc to eliminate blockage Purge the pump Rinse the check valve with ultrasonic and reinstall or replace it Replace the plunger rod seal Reconnect the tubings Adjust the flow rate Rinse the degassing channels Use fresh and appropriate solvents Flush the system using an appropriate solvent 5-8 L-3200 Series Pump Service Guide

73 Chapter 6 Firmware Update RIGOL Chapter 6 Firmware Update If the software version impacts the equipment performance, please update the software. Here, firmware update refers to software update, namely DSP update. Note: Please first install Framework.Net 2.0 or higher on the PC. Operation steps: 1. Turn off the equipment. 2. Open the Bootloader software (the interface is as shown in Figure 6-1) and select the communication port currently used in the 通信端口 (Communications Port)" dropdown menu. Figure 6-1 Bootloader Software Interface 3. Click 导入升级文件 (import the update file)", the dialog box as shown in Figure 6-2 is displayed and select the desired program for update. L-3200 Series Pump Service Guide 6-1

74 RIGOL Chapter 6 Firmware Update Figure 6-2 Import the Update File Interface 4. Click 升级固件 (update firmware)" and then turn on the equipment. Observe the progress bar of file transmission and when the progress of file transmission becomes 100%, the upgrade is completed and the dialog box as shown in Figure 6-3 will be displayed. Figure 6-3 Update Completed Dialog Box 6-2 L-3200 Series Pump Service Guide