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1 Ros Muc, Connemara, Co.Galway Testing Laboratory Registration number: 108T is accredited by the Irish National Board (INAB) to undertake testing as detailed in the Schedule bearing the Registration Number detailed above, in compliance with the International Standard ISO/IEC 17025: nd Edition General Requirements for the Competence of Testing and Calibration Laboratories (This Certificate must be read in conjunction with the Annexed Schedule of ) Date of award of accreditation: 17:12:2001 Date of last renewal of accreditation: 09:08:2011 Expiry date of this certificate of accreditation: 09:08:2016 This shall remain in force until further notice subject to continuing compliance with INAB accreditation criteria, ISO/IEC and any further requirements specified by the Irish National Board. Manager: Chairperson: Dr Adrienne Duff Issued on 05 June 2012 Mr Tom O Neill Organisations are subject to annual surveillance and are re-assessed every five years. The renewal date on this Certificate confirms the latest date of renewal of accreditation. To confirm the validity of this Certificate, please contact the Irish National Board. The INAB is a signatory of the European co-operation for (EA) Testing Multilateral Agreement (MLA) and the International Laboratory Cooperation (ILAC) Mutual Recognition Arrangement. Edition 28, 15/01/ T Page 1 of 39

2 Schedule of Wilton Park House, Wilton Place, Dublin 2, Ireland Tel Fax inab@inab.ie Web (Annex to Certificate) COMPLETE LABORATORY SOLUTIONS Chemical and Biological Testing Laboratory Initial Registration Date : 17-December-2001 Postal Address: Ros Muc Unit 3 (Address of other locations Connemara IDA Enterprise Park as they apply) Co Galway Tuam Road, Galway Telephone: +353 (0) (0) Fax: +353 (0) info@cls.ie Contact Name: Olivia Ryan Facilities: Public testing service Edition 28, 15/01/ T Page 2 of 39

3 Wilton Park House, Wilton Place, Dublin 2, Ireland Tel Fax inab@inab.ie Web Schedule of THE IRISH NATIONAL ACCREDITATION BOARD (INAB) is the Irish body for the accreditation of organisations including laboratories. Laboratory accreditation is available to testing and calibration facilities operated by manufacturing organisations, government departments, educational institutions and commercial testing/calibration services. Indeed, any organisation involved in testing, measurement or calibration in any area of technology can seek accreditation for the work it is undertaking. Each accredited laboratory has been assessed by skilled specialist assessors and found to meet criteria which are in compliance with ISO/IEC or ISO/IEC (medical laboratories). Frequent audits, together with periodic inter-laboratory test programmes, ensure that these standards of operation are maintained. Testing and Calibration Categories: : Permanent laboratory calibration and testing where the laboratory is erected on a fixed location for a period expected to be greater than three years. Category B: Category C: Category D: Site calibration and testing that is performed by staff sent out on site by a permanent laboratory that is accredited by the Irish National Board. Site calibration and testing that is performed in a site/mobile laboratory or by staff sent out by such a laboratory, the operation of which is the responsibility of a permanent laboratory accredited by the Irish National Board. Site calibration and testing that is performed on site by individuals and organisations that do not have a permanent calibration/testing laboratory. Testing may be performed using (a) (b) (c) (d) portable test equipment a site laboratory a mobile laboratory or equipment from a mobile or site laboratory Standard Specification or Test Procedure Used: The standard specification or test procedure that is accredited is the issue that is current on the date of the most recent visit, unless otherwise stated. Glossary of Terms Facilities: Public calibration/testing service: Conditionally available for public calibration/testing: Normally not available for public calibration/testing: Commercial operations which actively seek work from others. Established for another primary purpose but, more commonly than not, is available for outside work. Unavailable for public calibration/testing more often than not. Laboratory users wishing to obtain assurance that calibration or test results are reliable and carried out to the Irish National Board criteria should insist on receiving an accredited calibration certificate or test report. Users should contact the laboratory directly to ensure that this scope of accreditation is current. INAB will, on request, verify the status and scope. Edition 28, 15/01/ T Page 3 of 39

4 Chemical Testing Laboratory, Ros Muc Waters Waters for potable and domestic purposes Sewage WWTP Effluent Trade wastes Bore Waters Other Waters Surface waters Biochemical oxygen demand Surface water: 1-7,000 mg/l Effluent: ph (4 10) Suspended solids (2 to 15,000 mg/l) Fats, oils and greases (5 to 10,000 mg/l) Phosphorous 2-7,000 mg/l (0 to 2,000 mg/l P0 4 -P) Orthophosphate (0.03 to 6,140 mg/l PO 4 ) Chloride (2.0 to 30,000 mg/l CI) Nitrite ( mg/l NO 2 -N) TON ( mg/l NO 3 -N) Documented in-house method based on Standard methods for the examination of water and waste water 22 nd Edition 2012 (unless otherwise stated) CLS 12 Measurement of Oxygen consumed over 5 days CLS 26 Measurement of electromotive force by electrode to determine Hydrogen ion concentration CLS 13 Increase in weight of sample filter dried at ºC CLS 25 Increase in weight after sample filtration and Soxhlet extraction Konelab CLS 35 Colormetric determinaton and adapted for discrete analyser Konelab CLS 36 Colormetric determination and adapted for discrete analyser Konelab CLS 37 Colormetric determination and adapted for discrete analyser Konelab CLS 38 Chemical reduction and colormetric determination. Adapted for discrete analyser Edition 28, 15/01/ T Page 4 of 39

5 Chemical Testing Laboratory, Ros Muc Waters Waters for potable and domestic purposes Sewage Trade wastes Bore Waters Other Waters Surface waters Nitrate ( mg/l NO 3- N) Ammonia (0.005 to 600 mg/l NH 3 -N) COD 10-30,000 mg/l Konelab CLS 39 (TON-NO 2 -N) Calc by discrete analyser Konelab CLS 40 Salicylate method based on Methods for the examination of water and associated Materials, Ammonia in waters, CLS 52 Based on Hach procedures manual 9th Edition Total Hardness 20-3,000 mg/l CaC0 3 Konelab CLS 77 Std Methods 22 nd Ed 2012, Colormetric determination and adapted for discrete analyser Sulphate 5-3,000 mg/l SO 4 Konelab CLS 88 based on Sulphate in Waters Effluents and Soils, 2nd edition (1988) Method E. Edition 28, 15/01/ T Page 5 of 39

6 Chemical Testing Laboratory, Ros Muc Waters Saline waters Biochemical Oxygen Demand Surface water: Effluent: 1-7,000 mg/l 2-7,000 mg/l CLS 12 Based on Standard Methods 22 nd Edition, Measurement of Oxygen consumed over 5 days. Suspended Solids (2 to 15,000mg/L) CLS 13 Based on Standard Methods 22 nd Edition, Increase in sample filter dried at ºC. COD 10-30,000 mg/l CLS 52 Based on Hach Procedures Manual 9th Edition Edition 28, 15/01/ T Page 6 of 39

7 Chemical Testing Laboratory, Ros Muc Waters Waters for potable and domestic purposes Trade wastes Bore waters Other waters Surface waters Cadmium 0.5ug - 5,000ug/L Chromium 0.5ug - 5,000ug/L Copper 1ug - 10,000ug/L Lead 0.5ug - 5,000ug/L Iron 10ug - 10,000ug/L Manganese 5ug - 5,000ug/L Magnesium 0.8mg - 800mg/L Calcium 3mg - 3,000mg/L Potassium 0.5mg mg/l Sodium 1mg - 1,000mg/L Zinc 5ug - 10,000ug/L Arsenic 0.5ug/L - 5,000ug/L Selenium 0.5ug/L - 5,000ugL Nickel 0.5ug/L - 5,000ug/L Aluminium 2ug/L - 10,000ug/L (20ug/L - 10,000ug/L for.05) Tin 0.5ug/L - 5,000ug/L Beryllium 0.5ug/L - 5,000ug/L Barium 0.5ug/L - 5,000ug/L Boron 10ug/L - 10,000ug/L Antimony 0.5ug/L - 5,000ug/L Molybdenum 0.5ug/L-5,000ug/L Cobalt 0.5ug/L - 5,000ug/L Strontium 5ug/L - 5,000ug/L Thallium 0.5ug/L- 5,000ug/L Tellurium 0.5ug/L - 5,000ug/L Vanadium 0.5ug/L - 5,000ug/L Documented in-house method based on: USEPA ICP-MS CLS129 Edition 28, 15/01/ T Page 7 of 39

8 Chemical Testing Laboratory, Ros Muc Waters Waters for potable and domestic purposes Trade wastes Colour, mg/l Equipment/techniques used In house method CLS29 based on Standard methods for examination of water and waste water 22 nd Edition, Saline Waters Turbidity, NTU CLS30, based on Standard methods for examination of.07 Bore waters water and waste water 22 nd Edition, Other waters Surface waters Total P, 0.05 to 1000mg/L PO4-P CLS151 based on ISO D11 (Macherey Nagel) Total Organic carbon (NPOC) CLS150 based on USEPA mg/l and Shimadzu User Manual for TOC V-CPH/CPN Total Nitrogen mg/l CLS 152 based on ISO Extractable Hydrocarbons by GC-FID CLS 147 method based on Diesel Range and Lube Oil (C8 to USEPA 8015B. C40) 10 to 10,000µg/l 200 to 10,000ug/l (Waste waters) Edition 28, 15/01/ T Page 8 of 39

9 Chemical Testing Laboratory, Ros Muc 766 Waters Waters for potable and domestic purposes Petrol Range Organics (PRO) (C5 to C12) Equipment/techniques used In house method CLS 148 based on USEPA 8015B Trade wastes Saline waters Bore waters Other waters Surface waters Benzene 10-10,000 μg/l Toluene 10-10,000 μg/l Ethylbenzene 10-10,000 μg/l o-xylene 10-10,000 µg/l m and p- Xylene 20-20,000 μg/l t-butyl methyl ether, 10-10,000 μg/l Edition 28, 15/01/ T Page 9 of 39

10 Chemical Testing Laboratory, Ros Muc Constituents of the environment Soils (Loam, Sand & Peat) Sediments Extractable Hydrocarbons by GC-FID Diesel Range and Lube Oil (C8 to C40) 50 mg/kg to 2,000 mg/kg 200 to 2000 mg/kg (Peat) Equipment/techniques used In house method CLS 156 and CLS 147, method adapted from USEPA 8015B Petrol Range Organics (PRO) (C5 to C12) 0.1 mg/kg to 169 mg/ kg CLS 157 and CLS 148 method adapted from USEPA 8015B 781 Constituents of the Benzene 0 mg/kg to 20 mg/kg In house methods Environment Toluene 0 mg/kg to 20 mg/kg CLS 157 and CLS Soils Ethylbenzene 0 mg/kg to 20 mg/kg adapted from USEPA (Loam, Sand & Peat ) m and p -Xylene 0.02 mg/kg to B.32 Sediments mg/kg o-xylene 0 mg/kg to 20 mg/kg t-butyl methyl ether, 0 mg/kg to 20 mg/kg Edition 28, 15/01/ T Page 10 of 39

11 Chemical Testing Laboratory, Ros Muc Waters Waters for potable and domestic purposes Other waters Surface waters Polycyclic Aromatic Hydrocarbons by HPLC Naphthalene Acenaphthylene Acenaphthene Fluorene Phenanthrene Anthracene Fluoranthene Pyrene ng/l ng/l ng/l (Surface water), ng/l (Drinking water) ng/l ng/l (Surface water), ng/l (Drinking water) Benzo (a) anthracene Chrysene Benzo (b) fluoranthene Benzo (k) fluoranthene Benzo (a) pyrene ng/l ng/l ng/l ng/l ng/l ng/l ng/l ng/l (Surface water), ng/l (Drinking water) Dibenzo (a,h) anthracene Benzo (g,h,i) perylene Indeno (1,2,3-cd) pyrene ng/l ng/l ng/l Equipment/techniques used CLS 149 PAH based on ISO and Agilent series G1321A user manual Edition 28, 15/01/ T Page 11 of 39

12 Chemical Testing Laboratory, Ros Muc INAB Classification number (P9) Waters Waters for potable and domestic purposes Trade wastes Bore waters Conductivity at 20 C, 5-12,730 µs/cm 25 C, 5-14,090 µs/cm In house method CLS 67 method based on Standard methods for the examination of water & wastewater, 22 nd Edition, Other waters Surface waters Waters for Potable Volatile Organic Compounds CLS 183 and Domestic Use (VOC) by GC-MS USEPA Adapted from Benzene 0.1 to 50µg/l Purge and Trap to Headspace 1,2 dichloroethane 0.1 to 50µg/l Injection.99 Other Waters (Drinking Water) Surface 1,2 dichloroethane 0.5 to 50µg/l (Surface Water) Tetrachloroethene 0.1 to 50 µg/l Trichloroethene 0.1 to 50µg/l Chloroform 1 to 50µg/l Bromoform 0.1 to 50µg/l (Drinking Water) Bromoform 0.5 to 50µg/l (Surface Water) Dibromochloromethane 0.1 to 50µg/l (Drinking Water) Dibromochloromethane 0.5 to 50µg/l (Surface Water) Edition 28, 15/01/ T Page 12 of 39

13 Chemical Testing Laboratory, Ros Muc INAB Classification number (P9) Waters Waters for potable and domestic purposes Other waters Surface waters Volatile Organic Compounds (VOC) by GCMS Bromodichloromethane 0.5 to 50µg/l Chlorodifluoromethane (CFC-22) 0.5 to 50 ug/l Dichlorodifluoromethane (CFC-12) 0.5 to 50 ug/l 1,3-butadiene 0.5 to 50ug/l Bromomethane (methyl bromide) 0.5 to 50ug/l Trichlorofluoromethane (CFC-11) 0.5 to 50ug/l Diethyl ether (ethyl ether) 1 to 50ug/l 1,1-dichloroethene 0.5 to 50ug/l Iodomethane (methyl iodide) 0.1 to 50 ug/l Methyl acetate 5 to 50 ug/l- (Drinking Water only) Carbon disulfide 0.5 to 50 ug/i Trans-1,2-dichloroethene 0.5 to 50 ug/l Methyl tert-butyl ether (MTBE) 2 to 50 ug/l 1,1-dichloroethane 0.5 to 50 ug/l Di-isopropyl ether (DIPE ) 0.5 to 50 ug/1 cis-1,2-dichloroethene 0.5 to 50 ug/l CLS 183 USEPA Adapted from Purge and Trap to Headspace Injection Edition 28, 15/01/ T Page 13 of 39

14 Chemical Testing Laboratory, Ros Muc INAB Classification number (P9) Waters Waters for potable and domestic use Other Waters Surface Bromochloromethane 0.5 to 50 ug/l (Drinking Water) Bromochloromethane 2 to 50 ug/l (Surface Water) Trichloromethane (chloroform) 1 to 50 ug/l Ethyl Tert-Butyl Ether (ETBE) 0.5 to 50 ug/l Tetrahydrofuran 5 to 50 ug/l 1,2-dichloroethane 0.1 to 50 ug/l (Drinking Water) 1,2-dichloroethane 0.5 to 50 ug/l (Surface Water) 1,1,1-trichloroethane 0.5 to 50 ug/l 1-chlorobutane (n-butyl chloride) 0.5 to 50 ug/l 1,1-dichloropropene 0.5 to 50 ug/l Carbon tetrachloride (tetrachloromethane) 0.5 to 50 ug/l Benzene 0.1 to 50 ug/l Tert-amyl methyl ether (TAME) 1 to 50 ug/l 1,2-dichloropropane 0.5 to 50 ug/l Trichloroethene 0.1 to 50 ug/l Bromodichloromethane 0.5 to 50 ug/l CLS 183 USEPA Adapted from Purge and Trap to Headspace Injection Edition 28, 15/01/ T Page 14 of 39

15 Chemical Testing Laboratory, Ros Muc INAB Classification number (P9) Waters Waters for potable and domestic use Other Surface Tert-amyl ethyl ether (TAEE) 5 to 50 ug/l cis -1,3-dichloropropene 1 to 50 ug/l - (Drinking Water Only) trans -1,3dichloropropene 1 to 50 ug/l - (Drinking Water Only) 1,1,2-trichloroethane 0.5 to 50 ug/l Toluene 2 to 50 ug/l 1,3-dichloropropane 0.1 to 50 ug/l (Drinking Water) 1,3-dichloropropane 0.5 to 50 ug/l - (Surface Water) 1,2-dibromoethane (EDB) 0.1 to 50 ug/l Tetrachloroethene 0.1 to 50 ug/l 1,1,1,2-tetrachloroethane 0.5 to 50 ug/i Ethylbenzene 2 to 50 ug/l. Tribromomethane (bromoform) 0.1 to 50 ug/l (Drinking Water) Tribromomethane (bromoform) 0.5 to 50 ug/l (Surface Water) Styrene 2 to 50 ug/l 1,1,2,2-tetrachloroethane 0.5 to 50 ug/l. o-xylene 2 to 50 ug/l 1,2,3-trichloropropane 0.5 to 50 ug/l Bromobenzene 1 to 50 ug/i CLS 183 USEPA Adapted from Purge and Trap to Headspace Injection Edition 28, 15/01/ T Page 15 of 39

16 Chemical Testing Laboratory, Ros Muc INAB Classification number (P9) Waters Waters for potable and domestic use Other Surface n-propylbenzene 1 to 50 ug/i 2-chlorotoluene 1 to 50 ug/i 4-chlorotoluene 0.5 to 50 ug/i Pentachloroethane 0.5 to 50 ug/l (Drinking Water) Pentachloroethane 2 to 50 ug/l (Surface Water) 1,3,5-trimethylbenzene (mesitylene) 2 to 50 ug/l tert-butylbenzene 1 to 50 ug/l 1,2,4-trimethylbenzene (pseudocumene) 2 to 50 ug/l 4-isopropyltoluene (p-cymene) 1 to 50 ug/l 1,2-dichlorobenzene 0.5 to 50 ug/l n -butylbenzene 2 to 50 ug/l Hexachloroethane 0.5 to 50 ug/l 1,2,4-trichlorobenzene 0.5 to 50 ug/l Naphthalene 1 to 50 ug/l Hexachlorobutadiene 0.5 to 50 ug/l 1,2,3-trichlorobenzene 0.5 to 50 ug/l m and p-xylene 4-60 µg/l Vinyl Chloride 0.1 to 50 µg/l CLS 183 USEPA Adapted from Purge and Trap to Headspace Injection Edition 28, 15/01/ T Page 16 of 39

17 Chemical Testing Laboratory, Ros Muc 766. Waters Waters for potable and domestic purposes Fluoride 0.1mg/l to 10mg/l Equipment/techniques used CLS 182 Based on USEPA Sewage Trade Waste Saline Bore Waters 0.2mg/l to 10mg/l 1.5mg/l to 10mg/l 0.1mg/l to 10mg/l.99 Other waters Surface water 0.2mg/l to 10 mg/l Edition 28, 15/01/ T Page 17 of 39

18 Biological Testing Laboratory, Ros Muc INAB Classification number (P9) Waters Bacteriological condition of potable water Enumeration of micro-organisms Colony count technique at 22 C, 30oC and 37 C in water Documented in-house method: CLS 95, based on The Microbiology of Drinking Water (2007) Part 7 Methods for the enumeration of heterotrophic bacteria by pour plate and spread techniques.12 Bacteriological condition of Enumeration of Pseudomonas CLS 44 based on industrial water aeruginosa The Microbiology of Drinking Water (2010) Part Micro Tests for Enumeration of total coliforms at CLS 16, Membrane filtration, based on Factory hygiene 37 C & E. coli at 44 C The Microbiology of Drinking Water (2009). Part 4 - purposes Methods for examination of water & associated materials..03 Water Enumeration of total coliforms at CLS 33, based on 37 C & E. coli at 44 C The Microbiology of Drinking water (2009) part 4 - (Colilert) Detection of Salmonella CLS 45, based on The Microbiology of Drinking Water (2006). Part 9 - Isolation of salmonella. Enumeration of Enterococci CLS 42, based on The Microbiology of Drinking Water (2010). Part 5 - Methods for examination of water and associated materials. Enumeration of Sulphite Reducing CLS 43, based on Clostridia and Clostridium The Microbiology of Drinking Water (2010). Part 6 - perfringens Methods for examination of water & associated materials. Edition 28, 15/01/ T Page 18 of 39

19 Biological Testing Laboratory, Ros Muc Waters including effluents Bacteriological condition of potable waters Detection and enumeration of Legionella spp Documented in-house method: CLS 100 based on ISO : Bacteriological condition of industrial waters (treated,recirculating) 818 Microbiological tests for factory hygiene purposes.03 Water 870 Waters including effluents.11 Bacteriological condition Enumeration of Legionella CLS 101 based on ISO of potable waters species in water and the 2:2008 detection of Legionella.12 Bacteriological condition pneumophila. of industrial waters Serogroups 1 and (treated,recirculating).15 Bacteriological condition of swimming pools and spas Edition 28, 15/01/ T Page 19 of 39

20 Biological Testing Laboratory, Ros Muc Aquatic Biology (Potable and environmental including surface waters, lakes, rivers and natural waters) Enumeration of free-living protozoa Detection and enumeration of Cryptosporidium oocysts Documented in-house method: CLS 139 based on US EPA 1623:2005 Edition 28, 15/01/ T Page 20 of 39

21 Biological Testing Laboratory, Ros Muc INAB Classification number (P9) 818 Micro Tests for factory hygiene purposes Surfaces Enumeration of micro-organisms at 30 C for 48 hrs Documented in-house method: CLS 15 based on ISEN ISO 4833:2003 Or CLS 46, based on BS5763 Part Enumeration of Staphylococcus aureus CLS 3, based on IS EN ISO Amd 2003 Detection of Salmonella CLS 2, based on ISO 6579:2002 amd 2007 Detection of Listeria monocytogeness Detection of Listeria monocytogenes by ALOA One Day method CLS 4, based on IS EN ISO :1996/A1:2004 CLS 163, based on AES ALOA One Day (AFNOR Cert: AES /00) Detection of Listeria species by ALOA One Day method CLS 164 based on AES ALOA One Day (AFNOR cert: AES 10/03/09/00) Enumeration of total coliforms CLS 8, based on ISO 4832:2006 Enumeration of E.coli CLS 9, based on ISO :2001 Edition 28, 15/01/ T Page 21 of 39

22 Biological Testing Laboratory, Ros Muc INAB Classification number (P9) 818 Micro Tests for factory hygiene purposes Surfaces Enumeration of Enterobacteriacae Documented in-house method: CLS 21, based on ISO :2004 Enumeration of TVCs (Contact Plate) CLS 80, based on ISO 18593:2004 Enumeration of Yeast and Mould CLS 1 based on ISO and 2:2008 Enumeration of TVC at 22, 30 37C by pour and spread plates by single plate CLS 132, CLS 133 (In house methods) Detection of E Coli 0157 Enumeration of Listeria species including L. monocytogens CLS 11 based on ISO16654:2001 CLS 159 based on reveal for E.Coli hour system. CLS 6 Based on IS EN ISO :1998/A1:2004 Edition 28, 15/01/ T Page 22 of 39

23 Biological Testing Laboratory, Ros Muc Micro Tests for Factory Hygiene purposes Air Enumeration of TVCs (Air Settlement Plate) CLS 82 (In-house method) Enumeration of yeast and moulds CLS 130 (In-house method) 811 Micro Biological Tests Documented in-house method: on Foods Enumeration of micro-organisms at CLS 15 (Pour Plate) based on Dairy Products 30 C IS EN ISO 4833:2003 Or CLS 46 (Spread plate), based on.03 Meat and meat BS 5763: Part products, game and poultry Enumeration of micro-organisms at CLS 50 (Pour plate), based on 37 C IS EN ISO 4833:2003 Or.02 Eggs and egg products CLS 49 (Spread plate), based on BS 5763: Part Confectionery Enumeration of micro-organisms at CLS 47 (Pour plate), based on.08 Fruit and vegetables 22 C IS EN ISO 4833:2003 Or CLS 48 (Spread plate) based on.23 Animal Feeds BS 5763: Part Pet Foods Enumeration of Staphylococcus CLS 3, based on aureus IS EN IS : 1999 Amd 2003 Edition 28, 15/01/ T Page 23 of 39

24 Biological Testing Laboratory, Ros Muc Micro Biological Tests on Foods Dairy Products Meat and meat products, game and poultry Eggs and egg products Cereals and Bakery Products Detection of Salmonella (Primary production samples included as per amd 1:2007 Enumeration of Enterobacteriaceae Detection of Listeria monocytogenes CLS 2 based on ISO 6579:2002 amd 1:2007 CLS 21, based on ISO :2004 CLS 4, based on IS EN ISO :1196/A1:2004 Detection of Listeria CLS 163, based on AES ALOA.15 Confectionary monocytogenes by ALOA One Day One Day (AFNOR cert: AES method (not.23 and.22 10/03-09/00).08 Fruit and vegetables Detection of Listeria species by CLS 164 based on AES ALOA.23 Animal Feeds ALOA One Day method (not.23 and One Day (AFNOR Cert AES.22) 10/03-09/00).22 Pet Foods Enumeration of E.coli CLS 9, based on ISO :2001 Enumeration of Clostridium CLS 7, based on perfringens ISO 7937:2004 Edition 28, 15/01/ T Page 24 of 39

25 Biological Testing Laboratory, Ros Muc 811 Micro Biological Tests on Foods Dairy Products Documented in-house method:.03 Meat and meat Enumeration of Bacillus cereus CLS 20 based in ISEN ISO products, game and 7932: 2004 poultry Enumeration of Pseudomonas CLS Eggs and egg products spp. (for only) Based on ISO13720: Cereals and Bakery products.15 Confectionary Enumeration of total coliforms CLS 8, based on ISO.08 Fruit and vegetables 4832: Animal Feeds.22 Pet Foods Edition 28, 15/01/ T Page 25 of 39

26 Biological Testing Laboratory, Ros Muc Micro Biological Tests on Foods Fish Shellfish and Molluscs Enumeration of micro-organisms at 30 C CLS 15 (Pour plate), based on IS EN ISO 4833:2003 Or CLS 46 (Spread plate), based on BS 5763: Part Enumeration off micro-organisms at 37 C CLS 50 (Pour Plate), based on IS EN ISO 4833: 2003 Or CLS 49 (Spread plate), based on BS 5763: Part Enumeration of micro-organisms at 22 C CLS 47 (pour plate), based on IS EN ISO 4833:2003 Or CLS 48 (Spread plate), based on BS 5763: Part Enumeration of Staphylococcus aureus CLS 3, based on IS EN ISO Amd 2003 Detection of Salmonella CLS 2, based on ISO 6579:2002, Amid 1:2007 Enumeration of Enterobacteriaceae CLS 21, based on ISO :2004 Edition 28, 15/01/ T Page 26 of 39

27 Biological Testing Laboratory, Ros Muc Micro Biological Tests on Foods Fish Shellfish and Molluscs Detection of Listeria monocytogenes CLS 4, based on IS EN ISO :1996/A1:2004 Detection of Listeria monocytogenes by ALOA One Day method CLS 163, based on AES ALOA One Day (AFNOR cert: AES 10/03-09/00) Detection of Listeria species by ALOA One Day method CLS 164 based on AES ALOA One Day (AFNOR Cert AES 10/03-09/00) Enumeration of Listeria Species including L. monocytogenes CLS 6 based on IS EN ISO :1998/A1:2004 Enumeration of Clostridium perfringens CLS 7, based on ISO 7937:2004 Enumeration of total coliforms CLS 8, based on ISO 4832:2006 Enumeration of E.coli using a MPN method (5 tubes, 3 dilutions) CLS 92 Based on CEFAS SOP Enumeration of E.coli in Molluscian Bivalve Shellfish, Issue 7 and ISO Edition 28, 15/01/ T Page 27 of 39

28 Biological Testing Laboratory, Ros Muc Micro Biological Tests on Foods Non-alcoholic beverages Enumeration of micro-organisms at 30 C Documented in-house method: CLS 15 (Pour plate), based on IS EN ISO 4833:2003 Or CLS 46 (Spread plate),based on BS 5763:Part Enumeration of micro-organisms at 37 C CLS 50 (Pour plate), based on IS EN ISO 4833:2003 Or CLS 49 (Spread plate),based on BS 5763:Part Enumeration of micro-organisms at 22 C CLS 47 (Pour plate), based on IS EN ISO 4833:2003 Or CLS 48 (Spread plate),based on BS 5763: Part Enumeration of Clostridium perfringens CLS 7, based on ISO 7937:2004 Enumeration of Pseudomonas spp CLS 22, based on ISO 13720:1995 Edition 28, 15/01/ T Page 28 of 39

29 Biological Testing Laboratory, Ros Muc Testing of Surfaces in abattoirs Meat surfaces Product contact Surfaces Enumeration of microorganisms at 30 ºC Enumeration of Enterobacteriaceae CLS 15, based on IS EN ISO 4833:2003 CLS 21, based on ISO :2004 Detection of Salmonella CLS 2, based on ISO 6579:2002, Amd 1:2007 Detection of Listeria monocytogenes by ALOA One Day method CLS 163 based on AES ALOA One Day (AFNOR cert: AES 10/03-09/00) Detection of Listeria species by ALOA One Day method CLS 164 based on AES ALOA One Day (AFNOR cert: AES 10/03-09/00) Edition 28, 15/01/ T Page 29 of 39

30 Biological Testing Laboratory, Ros Muc Microbiological tests on foods Dairy Products Eggs and egg products Meat and meat products, game and poultry Fish, shellfish and molluscs Fruit and vegetables Non-alcoholic beverages Pet Foods Animal Feeds Enumeration of 22,30, 37ºC by pour and spread plates by single plate Enumeration of Enterobacteriaceae by single plate (Except & ) Detection of Salmonella (Except 818, ) CLS 132, CLS 133 (In-house methods) CLS 134 (In-house method) CLS 131 based on Bioline Optima Elisa Edition 28, 15/01/ T Page 30 of 39

31 Biological Testing Laboratory, Ros Muc Microbiological tests on foods Dairy Products Cereals and bakery products Fruit and vegetables Non-alcoholic beverages Prepared dishes Enumeration of Yeast and Mould CLS 1 based on and 2: Microbiological tests CLS 11 based on ISO on foods Detection of Ecoli :2001 Dairy Products CLS159 based on reveal for.03 Meat and meat E. Coli hour system products,game and poultry Detection of Listeria CLS 163 based on AES ALOA.06 Soups, broths and monocytogenes by ALOA One One Day (AFNOR cert: AES sauces Day method 10/03-09/00).07 Cereals and bakery products Detection of Listeria species by CLS 164 based on AES ALOA AlOA One Day method One Day (AFNOR cert: AES.17 Prepared dishes 10/03-09/00) Edition 28, 15/01/ T Page 31 of 39

32 Biological Testing Laboratory, Ros Muc Microbiological tests on foods Dairy Products Eggs and egg products Meat,meat products, game and poultry Detection of Campylobacter spp CLS 181 based on ISO Fish and shellfish Soups, broths and sauces Cereals and bakery products Fruit and Vegetables.15 Confectionary.17 Prepared Plates 818 Microbiological Tests for Factory Hygiene Purposes Surfaces Edition 28, 15/01/ T Page 32 of 39

33 Biological Testing Laboratory, Ros Muc Microbiological tests on foods Dairy Products Foodstuffs intended for special nutritional use Detection of Enterobacter sakazakii CLS 172 based on ISO/TS Microbiological tests on Foods Dairy Products Enumeration of Listeria Species CLS 6 Based on IS EN ISO including L. monocytogenes :1998/A1: Eggs and egg products.03 Meat and meat products, game and poultry.07 Cereals and Bakery Products.08 Fruit and Vegetables.15 Confectionary Edition 28, 15/01/ T Page 33 of 39

34 Biological Testing Laboratory, Ros Muc Microbiological Tests on Foods Cereals and Bakery Products Enumeration of microorganisms at 30ºC Documented In-House method: CLS15 (Pour Plate) based on IS EN ISO 4833:2003 based on BS 5763: Part Enumeration of Staphylococcus aureus CLS 3 based on IS EN ISO :1999 And 2003 Edition 28, 15/01/ T Page 34 of 39

35 Biological Testing Laboratory, Ros Muc Microbiological Tests on Foods Soups Broths and Sauces Prepared Dishes Enumeration of microorganisms at 30ºC Enumeration of Staphylococcus aureus Enumeration of Enterobacteriaceae Documented In-House method: CLS15 (Pour Plate) based on IS EN ISO 4833:2003 CLS 3 based on IS EN ISO :1999 Amd 2003 CLS 21 based on ISO :2004 Detection of Salmonella CLS2 based on ISO 6579:2002, Amd 1:2007 Enumeration of Bacillus cereus CLS 20 based on IS EN ISO 7932:2004 Enumeration of E.coli CLS 9 based on ISO :2001 Detection of Listeria monocytogenes CLS 4 based on IS EN ISO :1996/A1:2004 Enumeration of Listeria species including L.monocytogenes CLS 6 based on IS EN ISO :1998/A1:2004 Edition 28, 15/01/ T Page 35 of 39

36 Biological Testing Laboratory, Galway Waters Bacteriological condition of potable water Bacteriological condition of industrial water Enumeration of micro-organisms Colony count technique at 22 C and 37 C in water Documented in-house method: CLS 95, based on The Microbiology of Drinking Water (2002) Part 7 Methods for the enumeration of heterotrophic bacteria by pour plate and spread techniques Bacteriological condition of environmental waters Microbiological tests for Factory Hygiene purposes Water Enumeration of Pseudomonas aeruginosa Enumeration of Total Viable Counts at 22 0 C, 35 0 C and 37ºC (for category.12 only, industrial waters only) CLS 44, based on The Microbiology of Drinking Water (2010) Part 8 CLS 137 fluid monitoring, membrane filtration based on HTM 2030 Part 3 testing of Endoscopy waters. CLS 160 fluid monitoring membrane filtration based on ISO 13959:2009 Water for Haemodialysis, USP 1230 Water for Haemodialysis Edition 28, 15/01/ T Page 36 of 39

37 Biological Testing Laboratory, Galway 818 Microbiological tests for Factory Hygiene purposes.03 Water Enumeration of total coliforms CLS 33 and E.Coli Colilert Idexx Quanti Tray method, based on The Microbiolgy of Drinking water (2009) part Waters CLS 16 Membrane filtration based on Microbiology of Drinking water.11 Bacteriological (2009) part 4. condition of potable water Enumeration of Enterococci CLS 42, based on The Microbiology of Drinking Water.12 Bacteriological (2010). Part 5 Methods for condition of industrial examination of water and water associated materials..16 Bacteriological Enumeration of sulphite CLS 43, based on condition of reducing Clostridia and The Microbiology of Drinking Water environmental waters Clostridium Perfingens (2010). Part 6 Methods for examination of water & associated materials. Edition 28, 15/01/ T Page 37 of 39

38 Biological Testing Laboratory, Galway 870 Waters.12 Bacteriological Enumeration of Total Viable CLS 171 condition of industrial C using membrane Based on: water filtration ISO :2006 Washer Disinfectors Part 1 and ISO :2008 Washer Disinfectors Part Microbiological Test for Factory Hygiene Purposes Surfaces Incubation and Enumeration of TSA Plates at 32.5 C In- house method: CLS Air Incubation and Enumeration of SDA Plates at 22.5 C In- house method: CLS 187 Edition 28, 15/01/ T Page 38 of 39

39 Biological Testing Laboratory, Galway 801 Tests on human pharmaceutical and biological products.10 Endotoxin Tests Endotoxin Testing of Purified, In-house method: CLS185 Water using Gel Clot Method Based on: ANSI/AAMI ST.99 Other Tests 72:2011 Bacterial Endotoxin test methodologies, routine monitoring and alternatives to batch testing Endotoxin Testing of Purified Water using Kinetic In-house method: CLS186 Turbidimetric Method Based on: ANSI/AAMI ST 72:2011 Bacterial Endotoxin test methodologies, routine monitoring and alternatives to batch testing Edition 28, 15/01/ T Page 39 of 39