The National Marine Biological Analytical Quality Control Scheme
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1 The National Marine Biological Analytical Quality Control Scheme Ring Test Bulletin Seagrass OMC RT05 Dr Emma Wells Wells Marine Surveys April
2 RING TEST DETAILS Ring Test Seagrass OMC RT05 Type /Contents - % cover estimations of seagrass Circulated - 4 th February 2014 Completion Date - 4 th March 2014 Number of Subscribing Laboratories - 13 Number of Participating Laboratories - 12 Total Number of Participants - 23 Number of Results Received Per Test - 16 (Test A), 20 (Test B), 11 (Test C) *multiple data entries per lab submitted 2
3 Test A (Open Quadrat) Table 1. Results for % cover estimations received from participating laboratories for test A. Lab Code MA2103a MA2103b MA2103c MA2103d MA2111 Image analysis results Test A Seagrass coverage % Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat MA2102a MA2102b MA2102c MA2102d MA2102e MA2112c MA2119 MA2131a MA2133 MA2134a MA2134b 3
4 Figure 1. Difference in % cover between submitted results and image analysis results, displayed as the average difference across all quadrats in test A for each participant. 4
5 Figure 2. Number of quadrat fails per participant based on z-scores derived from mean % cover estimates for test A. Figure 3. Number of quadrat fails per participant based on z-scores derived from % cover as calculated using image analysis for test A. 5
6 Test B (9 x 9 Gridded Quadrat) Table 2. Results for % cover estimations received from participating laboratories for test B. Lab Code Image analysis results MA2117e MA2117d MA2117c MA2103a MA2103b MA2103c MA2103d Test B Seagrass coverage % Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat MA2102b MA2102c MA2102e MA2112c MA2109a MA2109b MA2109c MA2110 MA2119 MA2131a MA2133 MA2134a MA2134b 6
7 Figure 4. Difference in % cover between submitted results and image analysis results, displayed as the average difference across all quadrats in test B for each participant. 7
8 Figure 5. Number of quadrat fails per participant based on z-scores derived from mean % cover estimates for test B. Figure 6. Number of quadrat fails per participant based on z-scores derived from % cover as calculated using image analysis for test B. 8
9 Test C (4 x 4 Gridded Quadrat) Table 3. Results for % cover estimations received from participating laboratories for test C. Lab Codes MA2103a MA2103b MA2103c MA2103d MA2111 MA2112c MA2119 MA2131a MA2133 MA2134a MA2134b Image analysis results Test C Seagrass coverage % Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat
10 Figure 7. Difference in % cover between submitted results and image analysis results, displayed as the average difference across all quadrats in test C for each participant. 10
11 Figure 8. Number of quadrat fails per participant based on z-scores derived from mean % cover estimates for test C. Figure 9. Number of quadrat fails per participant based on z-scores derived from % cover as calculated using image analysis for test C. 11
12 Table 4. Summary of results from tests A, B and C including minimum values recorded, maximum, mean and range per quadrat across all participants Results from Test A Results from Test B Results from Test C Average deviation mean from actual StDev Range Min Max Mean Avergae deviation mean from actual StDev Range Min Max Mean Average deviation mean from actual StDev Range image analysis seagrass coverage % Min Max Mean Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Quadrat Average Average Average
13 Table 5. Comparison of estimated results from tests A, B and C with image analysis % cover results. The value represents the average deviation from image analysis % cover value for each participant. Lab Code Test A Test B Test C MA2117e 9.32 MA2117d MA2117c 8.82 MA2103a MA2103b MA2103c MA2103d MA MA2102a 7.23 MA2102b MA2102c MA2102d 9.08 MA2102e MA2112c MA2109a MA2109b 8.82 MA2109c 9.87 MA MA MA2131a MA MA2134a MA2134b
14 Figure 10. Comparison of deviation in image analysis % cover from estimated % cover between quadrats highlighting those problematic quadrats. (Quadrats are arranged in order of lowest to highest % cover). 14
15 Image Analysis Results Areas of seagrass coverage as calculated using imagej analysis. Summary results for each quadrat include the total number of failures across all three tests as calculated using Z-scores based on both the mean % cover across all participants and the actual % cover as determined by image analysis. Quadrat: 1 Actual % cover: Total No. of fails from Z-score of mean: 3 Total No. of fails from Z-score of actual % cover: 2 Average deviation across 3 tests: 7.84 Quadrat: 2 Actual % cover: Total No. of fails from Z-score of mean: 1 Total No. of fails from Z-score of actual % cover: 13 Average deviation from mean:
16 Quadrat: 3 Actual % cover: Total No. of fails from Z-score of mean: 1 Total No. of fails from Z-score of actual % cover: 6 Average deviation from mean: 8.73 Quadrat: 4 Actual % cover: Total No. of fails from Z-score of mean: 2 Total No. of fails from Z-score of actual % cover: 4 Average deviation from mean:
17 Quadrat: 5 Actual % cover: Total No. of fails from Z-score of mean: 2 Total No. of fails from Z-score of actual % cover: 5 Average deviation from mean: Quadrat: 6 Actual % cover: Total No. of fails from Z-score of mean: 2 Total No. of fails from Z-score of actual % cover: 42 Average deviation from mean:
18 Quadrat: 7 Actual % cover: Total No. of fails from Z-score of mean: 1 Total No. of fails from Z-score of actual % cover: 6 Average deviation from mean: Quadrat: 8 Actual % cover: Total No. of fails from Z-score of mean: 2 Total No. of fails from Z-score of actual % cover: 3 Average deviation from mean:
19 Quadrat: 9 Actual % cover: Total No. of fails from Z-score of mean: 1 Total No. of fails from Z-score of actual % cover: 6 Average deviation from mean: Quadrat: 10 Actual % cover: Total No. of fails from Z-score of mean: 1 Total No. of fails from Z-score of actual % cover: 10 Average deviation from mean:
20 Quadrat: 11 Actual % cover: 7.87 Total No. of fails from Z-score of mean: 2 Total No. of fails from Z-score of actual % cover: 5 Average deviation from mean: 6.82 Quadrat: 12 Actual % cover: Total No. of fails from Z-score of mean: 2 Total No. of fails from Z-score of actual % cover: 3 Average deviation from mean:
21 Quadrat: 13 Actual % cover: Total No. of fails from Z-score of mean: 1 Total No. of fails from Z-score of actual % cover: 2 Average deviation from mean: 8.21 Quadrat: 14 Actual % cover: Total No. of fails from Z-score of mean: 0 Total No. of fails from Z-score of actual % cover: 22 Average deviation from mean:
22 Quadrat: 15 Actual % cover: Total No. of fails from Z-score of mean: 3 Total No. of fails from Z-score of actual % cover: 1 Average deviation from mean:
The National Marine Biological Analytical Quality Control Scheme
The National Marine Biological Analytical Quality Control Scheme www.nmbaqcs.org Ring Test Bulletin Macroalgae OMC RT05 Dr Emma Wells Wells Marine Surveys April 2014 Email: emma@wellsmarine.org 1 RING
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