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2 Table of Contents Products and Services... 2 GC Phases... 9 QC Specifications Non-Bonded Durabond (DB) PLOT/Gas Solid Phases Solvent Retention Data Chromatograms Environmental Food, Flavor, Life Science Petroleum and Industrial GC Accessories Conversion Kits Ferrules Flowmeters Gas Purifiers (Traps) Gas Handling Liners Septa Standards Tools Tubing and Tubing Accessories Unions Solid Phase Extraction (SPE) How to Find Us Indices General Index Compound Index Part Number Index www. 1 common final-ap.p65 1 3/13/01, 11:30 AM

3 Outstanding Technical Support. More than Knowledge. It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Know-how. To receive free technical support on all of these products, please contact your authorized Agilent Distributor or local Agilent Technologies office (see page 138 for a full listing). Our worldwide sales network is assisted and educated by our knowledgable team of technical support engineers led by Allen Vickers. Allen and his team have over 100 years of chromatography experience and are located at our sole GC column manufacturing site in Folsom California. Being physically down the hall from where the HP and DB capillary columns are made allows technical support engineers to provide up to the minute answers and solutions to your toughest chromatographic questions. No matter where you turn for help, within Agilent s worldwide sales network, rest assured that the Technical Support Team is there to back you up - just as we ve done for 25 years under the name J&W Scientific. Allen Vickers Technical Support Group Manager Angelica Reese Technical Support Engineer Mitch Hastings Sr. Technical Support Engineer Jason Ellis Technical Support Engineer Eberhardt Kuhn Sr. Technical Support Engineer Cameron George Sr. Technical Support Engineer 2 To locate your nearest authorized Agilent distributor please visit our web site www. To locate your nearest Agilent Technologies office see page 138. www. v finalunpriced-ap.p65 2 2/23/01, 8:53 AM

4 More than Answers. In addition to providing world class in-house technical support Agilent technical support chemists frequently travel to share their knowledge with customers around the world. Solutions. Seminars at Your Site. And on the Web. The Agilent technical team delivers service, support and seminars at convenient locations around the world. Emerging and experienced chromatographers alike will learn how to apply practical theories and insightful concepts to the GC laboratory. These informative, professional seminars can also be customized to your lab s specific requirements and presented at your site. Can t Get Away from the Lab? We ll bring our knowledge to you through our web seminars! Several times a month we offer eseminars that you can attend from wherever you are.at the office.at home.or out of town. All you need is a telephone, Internet access and a browser. Each seminar is presented by one of our experienced technical support engineers with whom you can interact with through the on-line chat and phone line. we loved the seminar. We have more than fifteen years of chromatography experience and we thought it was great. It was very convenient that we did not have to travel anywhere. e-seminar Schedule and Registration: All you need to do is visit click on Events in the left navigation bar and then click on e-seminars. Then you can click on View Scheduled Events to see all seminars for the next few months. For registration, please click on Attendee Log in and click Register for a new account. Now you can log in with your User ID and Password. For more information, please contact your local Agilent Office or your Authorized Distributor. See page 138. www. 3 common final-ap.p65 3 3/13/01, 11:34 AM

5 GC Troubleshooting Troubleshooting Basics Evaluating the Problem The first step in any troubleshooting effort is to step back and evaluate the situation. Rushing to solve the problem often results in a critical piece of important information being overlooked or neglected. In addition to the problem, look for any other changes or differences in the chromatogram. Many problems are accompanied by other symptoms. Retention time shifts, altered baseline noise or drift, or peak shape changes are only a few of the other clues that often point to or narrow the list of possible causes. Finally, make note of any changes or differences involving the sample, solvents, vials, pipettes, storage conditions, sample age, extraction or preparation techniques, or any other factor influencing the sample environment can be responsible. Checking the Obvious A surprising number of problems involve fairly simple and often overlooked components of the GC system or analysis. Many of these items are transparent in the daily operation of the GC and are often taken for granted (set it and forget it). The areas and items to check include: Gases - pressures, carrier gas average linear velocity, and flow rates (detector, split vent, septum purge). Temperatures - column, injector, detector and transfer lines. System parameters - purge activation times, detector attenuation and range, mass ranges, etc. Gas lines and traps - cleanliness, leaks, expiration. Injector consumables - septa, liners, O-rings and ferrules. Sample integrity - concentration, degradation, solvent, storage. Syringes - handling technique, leaks, needle sharpness, cleanliness. Data system - settings and connections. Baseline Instability or Disturbances Possible Cause: Injector contamination. Solution: Clean the injector. Comments: Try a condensation test; gas lines may also need cleaning. Possible Cause: Column contamination. Solution: Bake-out the column. Comments: Limit bake-out to 1-2 hours. Possible Cause: Unequilibrated detector. Solution: Allow the detector to stabilize. Comments: Some detectors may require up to 24 hours to fully stabilize. Possible Cause: Incompletely conditioned column. Solution: Fully condition the column. Comments: More critical for trace level analysis. Possible Cause: Change in carrier gas flow rate during the temperature program. Solution: Normal in many cases. Comments: MS, TCD and ECD respond to changes in carrier gas flow rate. 4 www. common final-ap.p65 4 3/13/01, 11:36 AM

6 Top Ten Habits for Gas Chromatographers. (from LC/GC, Vol. 15, No. 2, February 1997, John V. Hinshaw, pages ) 1. Obtain preinstallation checklists from instrument manufacturers and follow them. 2. Use a new ferrule whenever you connect a column. See page Use the correct grade of gas. 4. Install high grade pressure regulators. 5. Install the best gas purifiers/traps that you can. See pages Inspect and change autosampler syringes often. 7. Change septa regularly. See page Inspect and change inlet liners frequently. See page Keep an accurate instrument log. 10. Change only one (variable) thing at a time. www. 5 common final-ap.p65 5 2/28/01, 1:33 PM

7 The J&W brand of technically advanced GC columns. Optimized for peak performance. Agilent invests heavily in R&D for superior-performing, low-bleed GC columns, gaining us respect industrywide as the low-bleed leader. We d rather be known for no bleed. Which is precisely why we re obsessed with reducing column bleed to the bare minimum and developing new stationary phase chemistries. Why all the concern about column bleed, you ask? Because column bleed is the degradation of the stationary-phase polymer that accelerates at higher temperature, elevating the baseline as the temperature rises to the column s upper thermal limit. Ultimately, the signal-to-noise ratio dictates analytical sensitivities and detection limits. Decrease the noise and the sensitivity will increase, which is a good thing. Get rid of column bleed and you help eliminate that noise that can decrease MS spectral integrity, reduce instrument uptime, decrease column lifetime and lower reproducibility at trace levels. Why low-bleed columns are a smart choice. If you perform trace analyses where sensitivity and instrument performance are important, column bleed (or the lack thereof) should be important to you. Many GC detectors are sensitive to contamination from bleed. By using low-bleed columns, these detectors require less maintenance. Due to the unique relationship between ion storage capacity of the trap and sensitivity levels, ion trap MS users will especially benefit from low-bleed columns. J&W brand s low-bleed GC columns also give you the advantages of superior inertness and increased upper temperature limits. What makes J&W Scientific low-bleed columns so special? In short, it s J&W s unique polymer chemistry and proprietary deactivation. Our MS columns utilize special surface deactivation and siloxane chemistries that enhance the chromatographic performance of siloxane polymers. To provide selectivities virtually identical to their conventional counterparts, we offer DB-5ms, DB-35ms, DB-17ms and DB-225ms. DB-1ms provides the same selectivity as the standard 100% dimethylpolysiloxane (DB-1 type) column. Plus, our unique polymer chemistry and proprietary deactivation techniques help us increase temperature limits (340/360 ), improving thermal stability and optimizing performance. Because the polysiloxane backbone of the stationary phase is optimized to yield the lowest-bleed column possible, DB-XLB doesn t have any conventional counterparts. You can gain sensitivity by replacing your general-purpose non-polar columns, such as 100%-dimethylpolysiloxane and 5%-diphenyl/95% dimethy - polysiloxane, with DB-XLB. Primary Quant Ion is hidden 276 Standard 35%- Phenyl 276 Primary Quant Ion is enhanced Figure 1. One nanogram of benzo[g,h,i]perylene (m/z 276) at 340 C on a standard 35%-phenyl column and on DB-35ms. The increased signal-to-noise ratio is apparent in the chromatograms, and the cleaner spectra will result in better peak identifications. www. common final-ap.p65 6 3/13/01, 7:29 PM

8 With the tightest QC specs in the business! We make more capillary GC columns than anyone else in the world. And we still build them with our consistently rigid performance criteria, such as separation efficiency, retention characteristics, peak height ratios and bleed rates. Better precision. For better results. J&W Scientific columns adhere to very tight retention factor specifications, helping ensure consistent retention from column to column. To assure separation reproducibility for the widest range of compounds, we measure at least three retention indices for each column. Narrow retention index ranges help ensure reproducible peak separations from column to column, while a wide range indicates a failure to precisely control stationary phase structure. Higher performance. And nice and narrow peaks. Theoretical plates per meter measures column efficiency. Columns with higher plate numbers generate the narrowest peaks, improving the resolution of closely eluting peaks. Paying close attention to the surface deactivation process and coating of the stationary phase is critical to obtain the highest number of theoretical plates. Increased sensitivity. With very low bleed levels. Bleed is the column s contribution to the background signal. The lowestbleed columns provide the best analytical sensitivity, which is why we devote substantial research to reduce column bleed. We work hard to create the widest range of low-bleed stationary phases. We ve also improved industry-standard DB-1 and DB-5 columns, resulting in even lower bleed levels. What Do Our Tighter Performance Criteria Do For You? Feature Advantage Benefit Narrow Highest level of Confidence in analytical results Retention Index column-to-column and Retention reproducibility Factor Window Minimal method adjustment when changing columns Excellent peak shape. Agilent s stringent QC performance evaluation tests provide the utmost confidence in the qualitative and quantitative results, especially for difficult to chromatograph compounds. We measure peak height ratios for acidic and basic compounds to ensure the highest column performance for the widest range and types of compounds. We also carefully monitor peak symmetry and tailing for a broad range of chemically active compounds. Improved sample throughput Reduced downtime Specify J&W chemistry for intra-company methodologies Confidence in method transfer and intra-company results Increased Highest level of Accurate quantification Plates per resolving power Potentially shorter run times Meter Improved sample throughput Lowest bleed Increased analytical Reduced detection limits sensitivity for all detectors Smaller samples Reliable compound identification Fast baseline stabilization Faster column exchange Excellent thermal stability Minimized conditioning time Increased sample throughput Increased column lifetime Reduced downtime Increased sample throughput www. 7 v finalunpriced-ap.p65 7 2/23/01, 9:00 AM

9 You want it? We ve got it and then some. Agilent continually strives to provide the best customer service available. This means providing you with high quality J&W columns that are available when you need them. We stock hundreds of different off the shelf column configurations in order to provide fast delivery. How can we do this? Virtually all of Agilent s phases are synthesized in-house by our highly experienced polymer chemists Our highly automated manufacturing process ensures outstanding reproducibility Every Agilent column is individually tested, and the results are packaged with each column And of course, a little bit of elf magic Still Can t Find What Your Looking For? Contact our Custom Column Shop. Sometimes you need a column that has non-standard dimensions. Even though we offer over 1100 readily available columns Agilent will design, build and test capillary GC columns for customers with special needs. We can create columns with nonstandard lengths or unusual film thickness. We also recognize that sometimes customers have specific column performance requirements for their applications that might not be met with standard test mixes. As a result, Agilent can also custom-test your columns with your desired test mixture and test conditions to meet your specific performance requirements. For more information, please contact your local Agilent office or your authorized Agilent Distributor (see page 138 or visit Dean Rood, Custom Column Shop Manager 8 www. common final-ap.p65 8 3/13/01, 11:56 AM

10 Phase Specifications and Order Guides Table of Contents QC Specifications Quality Control Specifications Non-Bonded CAM Carbowax 20M OV Cyclodex-B CycloSil-B DX-1, DX-3, DX SE Series PLOT/Gas Solid Phases GS-Alumina GS-Alumina KCI GS-CarbonPLOT GS-GasPro HP-PLOT Molesieve GS-Q Durabond (DB) DB-1ht DB-5ht DB-17ht DB DB-1ms DB DB-5ms DB DB DB-17ms DB DB DB-35ms DB DB DB DB-225ms DB DB DB DB DB DB-1701P DB DB-ALC1 & DB-ALC DB-Dioxin DB-EVDX DB-FFAP DB-HT Sim Dis DB-MTBE DB-Petro DB-ProSteel DB-TPH DB-VRX DB-WAX DB-WAXetr DB-XLB Dual Columns v pricedfinal-ap.p65 9 3/13/01, 11:59 AM

11 Phase Specifications and Order Guides The Tightest QC Specifications in the Industry Highest level of column-to-column reproducibility Decreased column bleed for increased sensitivity All J&W columns are individually tested and must live up to our stringent QC requirements. QC specifications for column bleed, retention index window and plates per meter are published for our most popular phases and column configurations. The individual bleed specifications represent the difference, in pico amperes of the FID response, measured at the upper temperature limit of the column and the isothermal test temperature (generally 110 C to 125 C). We strive for the lowest bleed GC columns in the industry. Quality The specifications for RI represent our narrow retention index windows. A small number means you get the highest level of column-to-column reproducibility. Call Technical Support for detailed information regarding retention index. Plates per meter is a measurement of the resolving potential for a GC column. We publish the minimum plate specifications that a column must have before it can become a J&W capillary column. Quality Control Specifications Inner Diameter (mm I.D.) Length (meter) Film Thickness (µm) Maximum Allowable Bleed ( pa) RI Tolerance (+/- I) Minimum Plates/Meter Temperature Limits ( C) Part Number (P/N) DB , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ DB-1ms , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ Price ($) 10 v pricedfinal_nofpo-ap.p /21/01, 9:26 AM

12 Phase Specifications and Order Guides Quality Control Specifications Inner Diameter (mm I.D.) Length (meter) Film Thickness (µm) Maximum Allowable Bleed ( pa) RI Tolerance (+/- I) Minimum Plates/Meter Temperature Limits ( C) Part Number (P/N) DB , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ DB-5ms , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ , to 325/ DB-5ms ITD , to 325/ Price ($) 11 v pricedfinal_nofpo-ap.p /21/01, 9:27 AM

13 Phase Specifications and Order Guides Quality Control Specifications Inner Diameter (mm I.D.) Length (meter) Film Thickness (µm) Maximum Allowable Bleed ( pa) RI Tolerance (+/- I) Minimum Plates/Meter Temperature Limits ( C) Part Number (P/N) DB-WAX , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ , to 250/ DB , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ , to 280/ DB-17ms , to 320/ , to 320/ , to 320/ , to 320/ , to 320/ , to 320/ DB-35ms , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ DB , to 300/ , to 300/ , to 300/ , to 300/ , to 300/ DB-225ms , to , to , to , to , to Price ($) 12 v pricedfinal_nofpo-ap.p /21/01, 9:28 AM

14 Phase Specifications and Order Guides Quality Control Specifications Inner Diameter (mm I.D.) Length (meter) Film Thickness (µm) Maximum Allowable Bleed ( pa) RI Tolerance (+/- I) Minimum Plates/Meter Temperature Limits ( C) Part Number (P/N) DB-XLB , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ , to 340/ Price ($) Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions you need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Jason Ellis Technical Support Engineer 13 v pricedfinal-ap.p /13/01, 12:25 PM

15 Phase Specifications and Order Guides CAM, Carbowax 20M and OV-351 CAM Replaces HP-Basicwax Base deactivated polyethylene glycol Specifically designed for amine analysis Excellent peak shape for primary amines Carbowax TM 20M Polyethylene glycol, MW 20,000 Equivalent to USP Phase G16 OV TM -351 Nitroterephthalic acid modified polyethylene glycol Equivalent to USP Phase G35 CAM Chromatograms Amines in Water page 91 Primary Amines page 91 CAM Order Guide Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 220/ to 220/ to 220/ to 220/ to 220/ to 220/ to 220/ to 220/ to 200/ to 200/ to 200/ Unlike DB-WAX, CAM, Carbowax 20M and OV-351 GC columns are not bonded and cross-linked, therefore we do not recommend solvent rinsing. Test Mix: CAM P/N: Carbowax 20M P/N: OV-351 P/N: Similar Phases: CAM: Stabilwax TM -DB, Carbowax Amine Carbowax 20M: DB-WAX, HP TM 20M OV-351: DB-FFAP Carbowax 20M Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 220/ to 220/ to 220/ to 220/ to 220/ to 220/ OV-351 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 220/ to 220/ What do J&W s tighter performance criteria do for you? The integrity of your analytical results are important. Don t be fooled by cheap imitations!!!! J&W s legendary commitment to product reliabilty and column to column repeatability has raised the level of quality chromatographers around the world demand. This world class performance is acheived by controlling all aspects of the manufacturing process from beginning to end. Feature Advantage Benefit Narrow Retention Highest level of column-to-column Confidence in analytical results Index & Retention reproducibility Factor Window Minimal method adjustment when changing columns Specify J&W chemistry for intra-company methodologies Improved sample throughput Reduced downtime Confidence in method transfer and intra-company results Increased Plates Highest level of resolving power Accurate quantification per Meter Improved sample throughput Potentially shorter run times Lowest bleed Increased analytical Reduced detection limits sensitivity for all detectors Smaller samples Reliable compound identification Fast baseline stabilization Faster column exchange Excellent thermal stability Minimized conditioning time Increased sample throughput Increased column lifetime Reduced downtime Increased sample throughput 14 v pricedfinal-ap.p /14/01, 9:39 PM

16 Phase Specifications and Order Guides Cyclodex-B Chiral separations without chiral specific derivatization Broad range of resolving potential Excellent peak shape Because Cyclodex-B GC columns are not bonded or cross-linked, we do not recommend solvent rinsing. Test Mix: Cyclodex-B P/N: Similar Phases: LIPODEX TM C, Rt-βDEXm TM, β-dex 110, β-dex 120, Cyclodex-B Chromatograms Chiral Standards page 84 Menthol page 84 Cyclodex-B Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 230/ to 230/ to 230/ to 210/ CycloSil-B Chiral separations without chiral specific derivatization CycloSil-B Chromatogram Citrus Flavored Carbonated Beverage page 83 Rosemary Oil page 83 New stationary phase for improved resolution of many chiral separations 30% heptakis (2,3-di-O-methyl-6-O-t-butyl dimethylsilyl)-β-cyclodextrin in DB-1701 Ideal for many chiral γ-lactones and terpenes Because CycloSil-B GC columns are not bonded or cross-linked, we do not recommend solvent rinsing. Test Mix: CycloSil-B P/N: Similar Phases: LIPODEX C, Rt-βDEXm, β-dex 110, β-dex 120 CycloSil-B Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 260/ to 260/ v pricedfinal_nofpo-ap.p /21/01, 9:28 AM

17 Phase Specifications and Order Guides DB-1ht 100% Dimethylpolysiloxane Nonpolar Specially processed for extended temperature limit of 400 C High temperature, polyimide-coated, fused silica tubing Excellent peak shape and faster elution times for high boilers Bonded and cross-linked Solvent rinsable Test Mix: DB-1ht P/N: Similar Phase: None O CH 3 Si Structure of Dimethylpolysiloxane DB-1ht Order Guide CH 3 100% Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to to to to For connecting guard columns to DB-1ht columns, we recommend stainless steel unions. DB-5ht (5%-Phenyl)-methylpolysiloxane Nonpolar Specially processed for extended temperature limit of 400 C High temperature, polyimide-coated, fused silica tubing Excellent peak shape and faster elution times for high boilers Bonded and cross-linked Solvent rinsable Test Mix: DB-5ht P/N: Similar Phase: None O CH 3 Si O DB-5ht Order Guide C 6 H 5 Si CH 3 95% C 6 H 5 5% Structure of Diphenyldimethylpolysiloxane Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to to to to For connecting guard columns to DB-5ht columns, we recommend stainless steel unions. 16 v pricedfinal_nofpo-ap.p /21/01, 9:29 AM

18 Phase Specifications and Order Guides DB-17ht (50%-Phenyl)-methylpolysiloxane Midpolarity Extended upper temperature limit of 365 C High temperature, polyimide-coated, fused silica tubing Excellent peak shape and faster elution times for high boilers Improved resolution for triglycerides Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Test Mix: DB-17ht P/N: Similar Phases: Rtx TM -65TG, BPX50 O CH 3 Si CH 3 O Structure of Diphenyldimethylpolysiloxane DB-17ht Chromatogram Butter Triglycerides page 76 DB-17ht Order Guide C 6 H 5 Si C 6 H 5 50% 50% made by bovine elves (mooooo) Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 340/ to 340/ to 340/ to 340/ to 340/ You want it, we ve got it! Agilent continually strives to provide the best customer service available. This means providing you with high quality J&W brand columns that are available when you need them. We stock over 1100 different part number configurations so when you place an order you ll get the column you need. How can we do this? Virtually all of J&W s phases are synthesized in-house by experienced polymer chemists Our highly automated manufacturing process ensures absolute reproducibility Every J&W column is individually tested, and the results are packaged with each column And of course, a little bit of elf magic 17 v pricedfinal-ap.p /13/01, 12:20 PM

19 Phase Specifications and Order Guides DB-1 100% Dimethylpolysiloxane Nonpolar Excellent general purpose column Wide range of applications Low bleed High temperature limit Bonded and cross-linked Solvent rinsable Wide range of column dimensions available Equivalent to USP Phase G2 Test Mix: Microbore ( mm I.D.) P/N: Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: Certain thick film or thin film configurations require different test mixtures than those listed above. Please call Technical Support for specific test mix information. Similar Phases: HP-1, HP-101, Ultra-1, SPB-1, CP-Sil 5CB, RSL-150, RSL-160, Rtx-1, BP-1, CB-1, OV-1, OV-101, 007-1(MS), SP-2100, SE-30, CC-1, CP-Sil 5CB MS, ZB-1 DB-1 Chromatograms Bergamont Oil page 79 EPA Air Analysis Compendium Method TO-14 Standard page 57 EPA Method 8330 page 61 Fragrance Reference Standard page 78 Geranium Oil page 79 Lavender Oil page 79 Regular Unleaded Gasoline (California Phase 1) Fast GC Run page 101 Rose Oil page 79 Rosemary Oil page 79 Volatile Sulfur page 94 Ylang Ylang Oil page 79 O CH 3 Si CH 3 100% Structure of Dimethylpolysiloxane Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions you need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page Cameron George, Sr. Technical Support Engineer v pricedfinal-ap.p /13/01, 8:03 PM

20 Phase Specifications and Order Guides DB-1 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 325/ to 325/ to 325/ A to 325/ to 325/ A to 325/ to 325/ to 325/ to 325/ to 325/ A to 325/ to 325/ to 325/ A to 325/ to 325/ to 325/ to 325/ H to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ E to 325/ to 325/ to 325/ A to 325/ to 325/ to 325/ E to 325/ to 325/ to 325/ A to 325/ to 325/ to 325/ E to 325/ E to 325/ to 300/ HC to 325/ to 325/ to 325/ E to 325/ to 280/ to 280/ to 325/ to 325/ D to 325/ to 325/ A to 325/ to 325/ F to 325/ to 325/ to 325/ E 457 DB-1 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 325/ to 280/ G to 280/ to 280/ to 280/ to 325/ to 325/ to 325/ F to 300/ C to 280/ to 325/ to 325/ to 325/ E to 325/ to 300/ B to 280/ G to 280/ to 280/ to 300/ to 260/ to 260/ to 300/ to 300/ * to 260/ * to 260/ to 300/ * to 260/ B to 260/ to 300/ H to 300/ HJ to 260/ HB to 325/ H to 340/ to 340/ to 320/ K to 300/ to 300/ J to 300/ to 260/ to 260/ to 300/ J to 260/ to 340/ to 320/ K to 300/ to 300/ D to 300/ J to 300/ to 260/ B to 260/ to 260/ to 260/ to 300/ to 300/ J to 300/ to 260/ to 260/ * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns 19 v pricedfinal_nofpo-ap.p /21/01, 9:30 AM

21 Phase Specifications and Order Guides DB-1ms 100% Dimethylpolysiloxane Identical selectivity to DB-1 Nonpolar Very low bleed characteristics Excellent general purpose column Improved signal-tonoise ratio for better sensitivity and mass spectral integrity 340/360 C Upper temperature limit Bonded and crosslinked Solvent rinsable Equivalent to USP Phase G2 O CH 3 Si CH 3 100% Structure of Dimethylpolysiloxane DB-1ms Chromatograms Drug Screen page 86 Environmental Analyte Inertness Mix page 68 EPA Method 8082 PCB Congeners page 71 DB-1ms Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ Test Mix: Capillary ( mm I.D.) P/N: Similar Phases: Rtx-1ms, CpSil5CBAS DB-5 (5%-Phenyl)-methylpolysiloxane Nonpolar Excellent general purpose column Wide range of applications Low bleed High temperature limit Bonded and cross-linked Solvent rinsable Wide range of column dimensions available Equivalent to USP Phase G27 Test Mix: Microbore ( mm I.D.) P/N: Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: Certain thick film or thin film configurations require different test mixtures than those listed above. Please call Technical Support for specific test mix information. Similar Phases: HP-5, Ultra-2, SPB-5, CP-Sil 8CB, RSL-200, Rtx-5, BP-5, CB-5, OV-5, 007-2(MPS-5), SE-52, SE-54, XTI-5, PTE-5, HP-5MS, CC-5, ZB-5 O CH 3 Si O C 6 H 5 Si CH 3 95% C 6 H 5 5% Structure of Diphenyldimethylpolysiloxane 20 v pricedfinal_nofpo-ap.p /21/01, 9:31 AM

22 Phase Specifications and Order Guides DB-5 Chromatograms CLP Pesticides page 65 Drugs page 87 Lemon Oil page 82 Over-the-Counter Pain Killers TMS Derivatives page 85 DB-5 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 325/ to 325/ to 325/ to 325/ E to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ H to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ E to 325/ to 325/ to 300/ C to 325/ to 325/ to 325/ E to 325/ to 325/ to 325/ to 325/ to 325/ E to 325/ to 325/ to 325/ to 325/ to 325/ E to 325/ to 325/ D to 325/ DB-5 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 325/ to 325/ F to 325/ to 325/ to 325/ E to 325/ to 300/ B to 325/ D to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ E to 325/ to 300/ to 300/ * to 300/ to 300/ * to 260/ to 300/ * to 280/ H to 260/ HB to 260/ H to 300/ K to 300/ to 300/ J to 300/ to 260/ to 300/ J to 280/ to 260/ to 300/ K to 300/ to 300/ D to 300/ J to 300/ to 260/ B to 260/ to 260/ to 300/ J to 280/ to 260/ to 300/ to 260/ * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns 21 v pricedfinal_nofpo-ap.p /21/01, 9:31 AM

23 Phase Specifications and Order Guides DB-5ms Replaces HP-5TA Equivalent to a (5%-Phenyl)-methylpolysiloxane Nonpolar Very low bleed characteristics Excellent inertness for active compounds Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Certified for MS Ion trap testing and certification available Close equivalent to USP Phase G27 Test Mix: DB-5ms P/N: Similar Phases: Rtx-5MS, HP-5MS, PTE-5, CP-Sil 8CBms, BPX-5 DB-5ms Chromatograms Amines and Nitriles page 91 EPA Method page 69 EPA Method 8061 (Phthalate Esters) page 68 EPA Method 8270 page 70 Organochlorine Pesticides (EPA Method 8081A) page 66 Organophosphorous Pesticides page 67 CH 3 CH 3 CH 3 O Si Si O Si DB-5ms Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ A to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ A to 325/ to 325/ to 325/ to 325/ to 300/ to 300/ to 300/ J to 300/ DB-5msITD Order Guide Ion Trap Tested Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 325/ CH 3 CH 3 CH 3 m n Structure of poly(dimethylsiloxy)poly(1,4-bis(dimethylsiloxy) phenylene)siloxane 22 v pricedfinal_nofpo-ap.p /21/01, 9:32 AM

24 Phase Specifications and Order Guides DB Equivalent to a (5%-Phenyl)-methylpolysiloxane Nonpolar Specially processed to exhibit excellent inertness for EPA Semivolatiles Methods 625, 1625, 8270 and CLP protocols* Surpasses EPA performance criteria for semivolatiles Inert for base, neutral and acidic compounds High temperature limit with excellent thermal stability and low bleed Bonded and cross-linked Solvent rinsable * Pentachlorophenol, 2,4-Dinitrophenol, Carbazole, and N-Nitrosodiphenylamine used to test response factors. DB Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 325/ to 300/ Test Mix: DB P/N: Similar Phases: XTI-5, Rtx-5, PTE-5 You want it, we ve got it! Agilent continually strives to provide the best customer service available. This means providing you with high quality J&W brand columns that are available when you need them. We stock over 1100 different part number configurations so when you place an order you ll get the column you need. How can we do this? Virtually all of J&W s phases are synthesized in-house by experienced polymer chemists Our highly automated manufacturing process ensures absolute reproducibility Every J&W column is individually tested, and the results are packaged with each column And of course, a little bit of elf magic 23 v pricedfinal-ap.p /13/01, 12:27 PM

25 Phase Specifications and Order Guides DB-17 (50%-Phenyl)-methylpolysiloxane Midpolarity Excellent for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G3 Test Mix: Microbore ( mm I.D.) P/N: Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: Certain thick film or thin film configurations require different test mixtures than those listed above. Please call Technical Support for specific test mix information. Similar Phases: HP-17, RSL-300, Rtx-50, CC-17, CP-Sil24CB (MPS-50), SP-2250, HP-50+, SPB-50, ZB-50 O CH 3 Si O C 6 H 5 Si CH 3 50% C 6 H 5 50% Structure of Diphenyldimethylpolysiloxane DB-17 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ H to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 260/ to 260/ to 260/ to 280/ to 280/ to 280/ to 260/ to 260/ to 280/ to 260/ to 260/ to 260/ to 260/ v pricedfinal-ap.p /13/01, 12:30 PM

26 Phase Specifications and Order Guides DB-17ms 320/340 C Upper temperature limit Equivalent to (50%-Phenyl)-methylpolysiloxane Very low bleed midpolarity Excellent inertness for active compounds Enhanced mass spectral integrity Bonded and cross-linked Solvent rinsable Best column for CLP pesticides Test Mix: DB-17ms P/N: Similar Phases: HP-50+, Rtx-50, , SP-2250, SPB-50, BPX-50, SPB-17 DB-17ms Chromatograms CLP Pesticides page 64 Hallucinogens page 87 DB-17ms Order Guide made by elves Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 320/ to 320/ to 320/ to 320/ to 320/ to 320/ to 320/ to 320/ to 320/ to 320/ Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions you need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Allen Vickers, Manager, Applied Sciences 25 v pricedfinal-ap.p /13/01, 8:04 PM

27 Phase Specifications and Order Guides DB-23 Replaces HP-23 (50%-Cyanopropyl)- methylpolysiloxane High polarity Designed for separation of fatty acid methyl esters (FAMEs) Excellent resolution for cis- and trans-fame isomers Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G5 Test Mix: Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: O CN CH 2 CH 2 CH 2 Si CH 3 Structure of Cyanopropylmethylpolysiloxane n DB-23 Chromatogram Fatty Acid Methyl Esters (FAMEs) page 73 DB-23 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 230/ to 230/ Similar Phases: SP-2330, Rtx-2330, , AT-Silar DB-35 (35%-Phenyl)-methylpolysiloxane Midpolarity Low bleed Inert to active solutes Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G42 DB-35 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 300/ to 300/ to 280/ to 300/ to 300/ to 300/ to 300/ to 280/ to 280/ to 260/ to 260/ to 260/ Test Mix: DB-35 P/N: Certain thick film or thin film configurations require different test mixtures than that listed above. Please call Technical Support for specific test mix information. O CH 3 Si O C 6 H 5 Si Similar Phases: Rtx-35, SPB-35, AT-35, Sup-Herb CH 3 65% C 6 H 5 35% Structure of Diphenyldimethylpolysiloxane 26 v pricedfinal_nofpo-ap.p /21/01, 9:34 AM

28 Phase Specifications and Order Guides DB-35ms Replaces HP-35ms Equivalent to a (35%-Phenyl)-methylpolysiloxane Midpolarity Very low bleed characteristics Extended temperature limit of 360 C Excellent inertness for active compounds Certified for MS Ion trap testing and certification available Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G42 Test Mix: DB-35ms P/N: DB-35ms Chromatograms Organochlorine Pesticides page 65 Organochlorine Pesticides (EPA Method 8081A) page 66 Organophosphorous Pesticides page 67 DB-35ms Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 320/ to 320/ Similar Phases: Rtx-35, SPB-35, AT-35, Sup-Herb, MDN v pricedfinal_nofpo-ap.p /21/01, 9:35 AM

29 Phase Specifications and Order Guides DB-200 Trifluoropropyl-methyl polysiloxane 300/320 C temperature limit Midpolarity (more polar than DB-1701 or DB-17) Ideal for difficult to separate positional isomers Unique interactions with compounds containing nitro, halogen and carbonyl groups Low ECD bleed Unique selectivity Close equivalent to USP Phase G6 Test Mix: DB-200 P/N: Certain thick film or thin film configurations require different test mixtures than that listed above. Please call Technical Support for specific test mix information. O CF 3 CH 2 CH 2 Si CH 3 Structure of Trifluoropropylmethylpolysiloxane n DB-200 Chromatograms Acrylate Impurities on DB-200 page 90 Aromatic Solvents page 92 DB-200 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 300/ to 300/ to 300/ to 300/ to 300/ to 300/ to 300/ to 300/ to 280/ to 280/ to 280/ to 280/ Similar Phase: Rtx-200 DB-210 Replaces HP-210 (50%-Trifluoropropyl)-methylpolysiloxane High polarity Excellent for U.S. EPA Methods 8140 and 609 Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G6 Test Mix: Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: Similar Phases: RSL-400, SP-2401 DB-210 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 240/ to 240/ to 240/ to 240/ to 240/ to 240/ to 240/ to 240/ to 240/ to 220/ * to 220/ to 220/ to 220/ * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns CF 3 CH 2 CH 2 O Si CH 3 100% Structure of Trifluoropropylmethylpolysiloxane 28 v pricedfinal_nofpo-ap.p /21/01, 9:35 AM

30 Phase Specifications and Order Guides DB-225 Replaces HP-225 (50%-Cyanopropylphenyl)-dimethylpolysiloxane DB-225 Chromatograms Alditol Acetates page 77 FAME Standard page 74 Mid/high polarity Excellent for separations of cis- and transfatty acid methyl esters (FAMEs) Low bleed Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G7 Test Mix: Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: Certain thick film or thin film configurations require different test mixtures than those listed above. Please call Technical Support for specific test mix information. DB-225 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 220/ to 220/ to 220/ to 220/ to 220/ to 220/ to 220/ to 220/ to 220/ to 200/ to 220/ to 200/ Similar Phases: HP-225, SP-2330, CP-Sil 43CB, RSL-500, Rtx-225, BP-225, CB-225, OV-225, DB-225ms Equivilent to (50%-Cyanopropylphenyl)- methylpolysiloxane Mid/high polarity Excellent for separations of cis- and transfatty acid methyl esters (FAMEs) Low bleed Bonded and cross-linked Solvent rinsable DB-225ms Chromatogram FAMEs page 75 DB-225ms Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to to to to to Close equivalent to USP Phase G7 Test Mix: Capillary ( mm I.D.) P/N: Similar Phases: HP-225, SP-2330, CP-Sil 43CB, RSL-500, Rtx-225, BP-225, CB-225, OV-225, v pricedfinal-ap.p /13/01, 1:33 PM

31 Phase Specifications and Order Guides DB Available in 105 meters for volatiles analyses DB Chromatogram Purgables Analysis (EPA Method 502.2) page 59 Excellent peak shape Bonded and cross-linked Solvent rinsable Test Mix: DB P/N: Certain thick film or thin film configurations require different test mixtures than that listed above. Please call Technical Support for specific test mix information. Similar Phases: Rtx-502.2, VOCOL, HP-VOC DB Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 260/ to 260/ to 260/280* to 260/280* A to 260/ to 260/ A * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns For the fastest and best resolution for EPA volatiles lists, see DB-VRX on page 39 DB-608 Specifically designed for the analysis of chlorinated pesticides and PCBs U.S. EPA Methods: 608, 508, 8080 Excellent inertness and recoveries without pesticide breakdown Fast analysis Bonded and cross-linked Solvent rinsable DB-608 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 280/ to 280/ to 260/ * to 260/ * to 260/ to 260/ to 260/ to 260/ * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns Test Mix: Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: Similar Phases: SPB-608, NON-PAKD Pesticide, , HP v pricedfinal_nofpo-ap.p /21/01, 9:36 AM

32 Phase Specifications and Order Guides DB-624 Replaces HP-624 Specifically designed for the analysis of volatile priority pollutants No cryogenics needed for U.S. EPA Method Excellent for U.S. EPA Methods: 501.3, 502.2, 503.1, 524.2, 601, 602, 8010, 8015, 8020, 8240, 8260 Excellent inertness for active compounds Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G43 Test Mix: DB-624 P/N: Improved Peak Shape! Similar Phases: VOCOL, NON-PAKD, AT-624, Rtx-Volatiles, Rtx-502.2, PE-624, , , HP-624, CP-624, ZB-624 DB-624 Chromatograms 1,3-Butadiene page 99 EPA Volatiles by GC/MS page 58 Extended Analyte List for EPA Method 8021 DB-624/VRX Dual Column page 62 Residual Solvents (USP <467>) page 85 DB-624 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to to to to to to to * to * to to to * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions you need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Cameron George, Sr. Technical Support Engineer 31 v pricedfinal-ap.p /13/01, 1:35 PM

33 Phase Specifications and Order Guides DB-1301 and DB-1701 Replaces HP-1301 and HP-1701 DB-1301: (6%-Cyanopropyl-phenyl) methylpolysiloxane DB-1301: Equivalent to USP Phase G43 DB-1701: (14%-Cyanopropyl-phenyl)-methylpolysiloxane Low/midpolarity Bonded and cross-linked Solvent rinsable Test Mix: DB-1301 Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: DB-1701 Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: Certain thick film or thin film configurations require different test mixtures than those listed above. Please call Technical Support for specific test mix information. Similar Phases to DB-1301: Rtx-1301, PE-1301, HP-1301 Similar Phases to DB-1701: SPB-1701, CP-Sil 19CB, Rtx-1701, BP-10, CB-1701, HP-1701, OV-1701, , ZB-1701, RH-1701 O CH 3 Si O CH 3 94% or 86% CN CH 2 CH 2 CH 2 Si Structure of Cyanopropyl-phenylmethylpolysiloxane DB-1701 Chromatograms C 6 H 5 6% or 14% Haloacetic Acid Methyl Esters page 61 TMS Sugars page 76 DB-1301 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 260/ to 260/ to 260/ to 260/ to 260/ DB-1701 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 280/ to 260/ * to 260/ * to 260/ to 260/ to 260/ to 260/ to 260/ to 260/ to 260/ to 260/ to 260/ * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns 32 v pricedfinal-ap.p /13/01, 1:41 PM

34 Phase Specifications and Order Guides DB-1701P Replaces HP-PAS1701 (14%-Cyanopropyl-phenyl)-methylpolysiloxane Low/midpolarity Specifically designed and processed for the analysis of organochlorine pesticides ECD tested to assure minimal pesticide breakdown and low ECD bleed Bonded and cross-linked Solvent rinsable Test Mix: Please contact J&W Technical Support for the test mix specifications and manufacturer's contact information. DB-1701P Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 280/ to 280/ to 260/ O CH 3 Si O CN CH 2 CH 2 CH 2 Si Similar Phases: SPB-1701, CP-Sil 19CB, Rtx-1701, BP-10, CB-1701, OV-1701, CH 3 86% C 6 H 5 14% Structure of Cyanopropyl-phenyl-methylpolysiloxane DB % Dimethylpolysiloxane Specifically designed for simulated distillation using ASTM Method D2887 Rapid conditioning, fast run time and low bleed when compared to packed columns Bonded and cross-linked Solvent rinsable Test Mix: DB-2887 P/N: Similar Phases: HP-1, Petrocol EX2887, MXT-2887, MXT-1 DB-2887 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to to O CH 3 Si CH 3 100% DB-2887 Order Guide Structure of Dimethylpolysiloxane 33 v pricedfinal_nofpo-ap.p /21/01, 9:36 AM

35 Phase Specifications and Order Guides DB-ALC1 & DB-ALC2 Reliable blood alcohol analysis Faster GC run times Improved resolution of key ethanol/acetone peaks Available in 0.32 and 0.53 mm I.D. Bonded and Cross-linked Test Mix: DB-ALC1 P/N: DB-ALC2 P/N: DB-ALC Chromatogram Blood Alcohols (Static Headspace/Split) page 86 DB-ALC1 & DB-ALC2 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price Phase (mm I.D.) (meter) (µm) ( C) (P/N) ($) DB-ALC to 260/ DB-ALC to 260/ DB-ALC to 260/ DB-ALC to 260/ Similar Phases: Rtx-BAC1, Rtx-BAC2 What do J&W s tighter performance criteria do for you? The integrity of your analytical results are important. Don t be fooled by cheap imitations!!!! J&W s legendary commitment to product reliabilty and column to column repeatability has raised the level of quality chromatographers around the world demand. This world class performance is acheived by controlling all aspects of the manufacturing process from beginning to end. Feature Advantage Benefit Narrow Retention Highest level of column-to-column Confidence in analytical results Index & Retention reproducibility Factor Window Minimal method adjustment when changing columns Specify J&W chemistry for intra-company methodologies Improved sample throughput Reduced downtime Confidence in method transfer and intra-company results Increased Plates Highest level of resolving power Accurate quantification per Meter Improved sample throughput Potentially shorter run times Lowest bleed Increased analytical Reduced detection limits sensitivity for all detectors Smaller samples Reliable compound identification Fast baseline stabilization Faster column exchange Excellent thermal stability Minimized conditioning time Increased sample throughput Increased column lifetime Reduced downtime Increased sample throughput 34 v pricedfinal-ap.p /14/01, 3:28 PM

36 Phase Specifications and Order Guides DB-Dioxin Specifically engineered for the analysis of polychlorinated dibenzodioxins (PCDDs) and dibenzofurans (PCDFs) Resolves 2,3,7,8-TCDD and 2,3,7,8-TCDF from all other isomers in one run Low bleed DB-Dioxin Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 250/ to 250/ to 250/ to 250/ Bonded and cross-linked Solvent rinsable Note: 100 psi regulator required to reach optimum carrier gas velocity Test Mix: DB-Dioxin P/N: Similar Phases: SP-2331, , Rtx-2332, CP-Sil 88 DB-EVDX Specially configured and tested for drugs of abuse confirmation DB-1-EVDX: 100% Dimethylpolysiloxane DB-5ms-EVDX: Equivalent to (5%-Phenyl)- methylpolysiloxane DB-EVDX Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price Phase (mm I.D.) (meter) (µm) ( C) (P/N) ($) DB-1-EVDX to 325/ DB-5ms-EVDX to 325/ DB-17-EVDX to 280/ DB-17-EVDX: (50%-Phenyl)-methylpolysiloxane Consistent retention and peak shape Test mix includes: caffenine, gluthethimide, lidocaine, phenobarbital, EDDP, Methaqualone, methadone, cocaine, desiprmine, carbamazepine Low bleed for GC/MS analysis Bonded and cross-linked Solvent rinsable Test Mix: Please contact Technical Support for the test mix specifications. 35 v pricedfinal-ap.p /21/01, 9:38 AM

37 Phase Specifications and Order Guides DB-FFAP DB-FFAP Chromatogram Nitroterephthalic acid modified polyethylene glycol Organic Acids page 76 High polarity Designed for the analysis of volatile fatty acids and phenols Replaces OV-351 Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G35 We do not recommend the use of water or methanol to rinse DB-FFAP GC columns. Test Mix: Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: Certain thick film or thin film configurations require different test mixtures than those listed above. Please call Technical Support for specific test mix information. Similar Phases: Stabilwax-DA, HP-FFAP, Nukol, 007-FFAP, BP21, CP-FFAP-CB DB-FFAP Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to to to to to to to to to to to to to to to to to to to to to * to H to to to to to to to to * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns 36 v pricedfinal-ap.p /21/01, 9:38 AM

38 Phase Specifications and Order Guides DB-HT Sim Dis Boiling point phase for high temperature simulated distillation Durable stainless steel tubing 430 C upper temperature limit Distillation range of C 6 to C 110+ Low bleed even at 430 C! Bonded and cross-linked Solvent rinsable Test Mix: DB-HT Sim Dis P/N: Similar Phases: Petrocol EX2887, CP-SimDist Ultimetal, MXT-2887, Rtx-2887, AC Controls High Temp Sim Dist, AT-2887 DB-HT Sim Dis Chromatogram n-paraffin Standard page 101 DB-HT Sim Dis Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 400/ DB-MTBE New low polarity stationary phase Resolves MTBE from 2-methylpentane and 3-methylpentane for better quantitation Engineered for purge & trap injection without the need for cryofocusing Bonded and cross-linked Solvent rinsable DB-MTBE Chromatogram Extended 8020 Analysis with MTBE page 56 DB-MTBE Order Guide DB-MTBE Order Guide made by xylenephone playing elves Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to * to * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns Test Mix: DB-MTBE P/N: Similar Phases: None 37 v pricedfinal-ap.p /21/01, 9:38 AM

39 Phase Specifications and Order Guides DB-Petro 100% Dimethylpolysiloxane Configured for the analysis of petroleum process products PONA, PIANO High resolution Bonded and cross-linked Solvent rinsable Note: 100 psi regulator required to reach optimum carrier gas velocity DB-Petro Chromatograms Regular Unleaded Gasoline (California Phase 1) page 100 DB-Petro Order Guide made by paleo-elves Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 325/ to 325/ A6 856 Test Mix: DB-Petro P/N: Similar Phases: Petrocol DH, HP-PONA, SPB-1, 007-1, Rtx-1, MXT-1 DB-TPH Specifically designed for the analysis of total petroleum hydrocarbons (TPHs) Three analyses in one injection: gas range organics, diesel range organics and motor oil Fast run time Bonded and cross-linked Solvent rinsable DB-TPH Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to to * 551 * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns Test Mix: DB-TPH P/N: Similar Phase: None 38 v pricedfinal-ap.p /21/01, 9:38 AM

40 Phase Specifications and Order Guides DB-VRX Low polarity Unique selectivity engineered for optimum resolution of volatiles analysis: U.S. EPA Meth- ods 502.2, and 8260 Test Mix: DB-VRX P/N: There s no substitute for the best! 0.45 mm I.D. columns provide more plates per meter compared to 0.53 mm I.D. columns for the fewest coelutions (an industry first)** No subambient cooling required to resolve the six gases Fast run time: < 30 minutes for optimum sample throughput < 8 minutes with 0.18 mm I.D. Excellent peak shape Bonded and cross-linked Solvent rinsable ** Two coelutions: 1) m- and p-xylene, for which U.S. EPA does not require separation, and 2) 1,1,2,2-tetrachloroethane and o-xylene which are separated by detectors PID and ELCD, respectively. Note to GC/MS analysts: These coeluting compounds have different primary characteristic ions of 83 and 106, respectively. DB-VRX Chromatograms EPA Volatiles by GC/MS (Split Injector) page 58 Extended Analyte List for EPA Method 8021 DB-624/VRX Dual Column page 62 DB-VRX Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to to to to to to * to * 1046 * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns Similar Phases: VOCOL, NON-PAKD, Rtx-Volatiles, PE-Volatiles, , HP-624, CP-624, Rtx-VRX, Rtx-VGC 39 v pricedfinal-ap.p /13/01, 8:16 PM

41 Phase Specifications and Order Guides DB-WAX Replaces HP-WAX Polyethylene glycol (PEG) Close equivalent to USP Phase G16 High polarity Lower temperature limit of 20 C is lowest of any bonded PEG phase, improves resolution of low boiling point analytes Column-to-column reproducability Bonded and cross-linked Solvent rinsable Test Mix: Microbore ( mm I.D.) P/N: Capillary ( mm I.D.) P/N: Megabore ( mm I.D.) P/N: Certain thick film or thin film configurations require different test mixtures than those listed above. Please call Technical Support for specific test mix information. Similar Phases: HP-20M, SUPELCOWAX 10, CP-WAX 52CB, SUPEROX II, CB-WAX, Stabilwax, BP-20, CC-WAX, 007-CW, Carbowax, HP-Innowax, Rtx-WAX, ZB-WAX H H C C O H H 100% Structure of Polyethylene Glycol DB-WAX Chromatograms Bergamont Oil page 81 FAME Standard page 74 Fatty Acid Methyl Esters page 73 Fragrance Reference Standard page 80 Geranium Oil page 81 Lavender Oil page 81 Rose Oil page 81 Rosemary Oil page 81 Turpentine Oil page 82 Ylang Ylang Oil page 81 DB-WAX Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ to 240/ to 250/ to 250/ to 240/ to 250/ to 250/ to 240/ to 250/ to 250/ to 240/ to 250/ to 250/ to 240/ to 250/ to 250/ to 240/ to 230/ * to 230/ * to 230/ to 230/ to 230/ to 230/ to 230/ to 230/ to 230/ * No special ferrules required for High Speed Megabore 0.45 mm I.D. columns 40 v pricedfinal-ap.p /21/01, 9:39 AM

42 Phase Specifications and Order Guides DB-WAXetr Polyethylene glycol (PEG) High polarity Extended temperature range (etr) Column-to-column repeatability Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G16 Test Mix: DB-WAXetr P/N: Certain thick film or thin film configurations require different test mixtures than that listed above. Please call Technical Support for specific test mix information. Similar Phases: HP-20M, SUPELCOWAX 10, CP-WAX 52CB, SUPEROX II, CB-WAX, Stabilwax, BP-20, 007-CW, Carbowax, HP-Innowax, ZB-WAX DB-WAXetr Chromatograms Impurities in Mixed Xylenes page 92 Organic Acids page 90 Organic Acids page 92 DB-WAXetr Order Guide Outstanding Technical Support made by high ranging elves Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 260/ to 260/ to 250/ to 260/ to 250/ to 260/ to 250/ to 260/ to 250/ to 250/ to 260/ to 250/ to 250/ to 250/ to 260/ to 250/ to 250/ to 250/ to 230/ to 240/ to 230/ to 230/ to 240/ to 230/ to 240/ to 230/ It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions you need. Just as we ve done for 25 years. Angelica Reese Technical Support Engineer Phone: (USA & Canada) Fax: Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page v pricedfinal-ap.p /13/01, 3:15 PM

43 Phase Specifications and Order Guides DB-XLB EXceptionally Low Bleed Low polarity Extended temperature limit of 360 C Unique selectivity Excellent inertness for active compounds Ideal for confirmational analyses Excellent for pesticides, herbicides, PCBs and PAHs Certified for MS Ion trap testing and certification available Bonded and cross-linked Solvent rinsable DB-XLB is designed for inhibiting column bleed at high temperatures. It also appears to have inadvertently inherited an exceptional ability for separating many PCB congeners when used with MS detection. This stellar performance was maximized after careful optimization of the column dimensions, temperature programs, and carrier gas flow conditions... (Frame, G. Analytical Chemistry News & Features, Aug. 1, 1997, 468A-475A) DB-XLB Chromatograms Aroclors (without 1221) page 72 CLP Pesticides page 64 Phenoxy Acid Methyl Esters page 61 Test Mix: DB-XLB P/N: Similar Phase: MDN-12 DB-XLB Order Guide made by exceptional elves Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 340/ to 320/ to 320/ to 320/ DB-XLB-ITD Ion Trap Tested Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 340/ v pricedfinal-ap.p /13/01, 3:17 PM

44 Phase Specifications and Order Guides DX-1, DX-3 and DX-4 DX-1: 90% Dimethylpolysiloxane - 10% Polyethylene Glycol DX-3: 50% Dimethylpolysiloxane - 50% Polyethylene Glycol DX-4: 15% Dimethylpolysiloxane - 85% Polyethylene Glycol Because DX series GC columns are not bonded and cross-linked, we do not recommend solvent rinsing. Test Mix: DX-1 P/N: DX-3 P/N: DX-4 P/N: DX-1 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 250/ to 250/ to 250/ DX-3 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 250/ to 250/ to 250/ DX-4 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 250/ to 250/ to 250/ to 250/ to 250/ to 250/ What do J&W s tighter performance criteria do for you? The integrity of your analytical results are important. Don t be fooled by cheap imitations!!!! J&W s legendary commitment to product reliabilty and column to column repeatability has raised the level of quality chromatographers around the world demand. This world class performance is acheived by controlling all aspects of the manufacturing process from beginning to end. Feature Advantage Benefit Narrow Retention Highest level of column-to-column Confidence in analytical results Index & Retention reproducibility Factor Window Minimal method adjustment when changing columns Specify J&W chemistry for intra-company methodologies Improved sample throughput Reduced downtime Confidence in method transfer and intra-company results Increased Plates Highest level of resolving power Accurate quantification per Meter Improved sample throughput Potentially shorter run times Lowest bleed Increased analytical Reduced detection limits sensitivity for all detectors Smaller samples Reliable compound identification Fast baseline stabilization Faster column exchange Excellent thermal stability Minimized conditioning time Increased sample throughput Increased column lifetime Reduced downtime Increased sample throughput 43 v pricedfinal-ap.p /21/01, 12:09 PM

45 Phase Specifications and Order Guides GS-Alumina A PLOT column for the analysis of light hydrocarbons Separates C 1 -C 4 saturated and unsaturated hydrocarbons Faster, more efficient and provides more sensitivity than packed equivalents Minimal conditioning time required* * Alumina columns have a tendency to adsorb water and CO 2 which, over time, results in changes in retention time. We use an advanced, proprietary deactivation process which allows for rapid regeneration. Fully water saturated GS-Alumina columns regenerate in 7 hours or less at 200 C. GS-Alumina Chromatograms Ethylene page 98 Ozone Precursor Analysis page 97 Propylene page 98 GS-Alumina Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) N/A -60 to N/A -60 to C is the lowest temperature used on this phase in our lab. Lower temperatures may be used depending on the sample. Test Mix: Test mixtures for PLOT columns are specially produced mixtures of light gases. Please contact Technical Support for the test mix specifications and manufacturer's contact information. Similar Phases: Al 2 O 3 /KCl, Al 2 O 3 /Na 2 SO 4, Rt-Alumina PLOT, Alumina PLOT GS-Alumina KCI Lower polarity (slightly different selectivity) than GS-Alumina Standard column choice for light hydrocarbon analysis Recommended phase for many ASTM methods GS-Alumina KCI Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) N/A -60 to N/A -60 to C is the lowest temperature used on this phase in our lab. Lower temperatures may be used depending on the sample. Exceptional resolution of propadiene and butadiene from ethylene and propylene streams Test Mix: Test mixtures for PLOT columns are specially produced mixtures of light gases. Please contact Technical Support for the test mix specifications and manufacturer's contact information. Similar Phases: CP-Alumina/KCl, Rt-Alumina 44 v pricedfinal-ap.p /14/01, 7:47 PM

46 Phase Specifications and Order Guides GS-CarbonPLOT High stability, bonded carbon layer stationary phase Unique selectivity for inorganic and organic gases Extended temperature limit of +360 C Test Mix: Test mixtures for PLOT columns are specially produced mixtures of light gases. Please contact Technical Support for the test mix specifications and manufacturer's contact information. Similar Phases: Carbopack, CLOT, Carboxen-1006 PLOT, CP-CarboPLOT P7 GS-CarbonPLOT Chromatograms Hydrocarbons page 93 Permanent Gases page 96 Solvents page 93 GS-CarbonPLOT Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 350/ to 350/ to 350/ to 350/ to 350/ to 350/ C is the lowest temperature used on this phase in our lab. Lower temperatures may be used depending on the sample. GS-GasPro Unique bonded PLOT column technology Excellent choice for light hydrocarbons and sulfur gases Retention stability not affected by water Separates CO and CO 2 on a single column Ideal PLOT column for GC/MS no particles Test Mix: Test mixtures for PLOT columns are specially produced mixtures of light gases. Please contact Technical Support for the test mix specifications and manufacturer's contact information. GS-GasPro Chromatograms C 1 and C 2 Halocarbons page 56 Mercaptans page 95 Ozone Precursor Analysis page 97 Permanent Gases in Refinery Gas page 96 Sulfur Gases in Light Hydrocarbon Stream page 95 Volatile Sulfur Compounds page 94 GS-GasPro Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) N/A -80 to 260/ N/A -80 to 260/ N/A -80 to 260/ N/A -80 to 260/ C is the lowest temperature used on this phase in our lab. Lower temperatures may be used depending on the sample. Similar Phase: CP-Silica PLOT 45 v pricedfinal-ap.p /21/01, 9:42 AM

47 Phase Specifications and Order Guides HP-PLOT Molesieve Replaces GS-Molesieve A PLOT column for the analysis of permanent gases O 2, N 2, CO and CH 4 resolve in less than 5 minutes Durable molecular sieve 5Å coating minimizes baseline spiking and damage to multiport valves For Ar/O 2 separation without cryogenic cooling, select a thick film Select thin film HP-PLOT Molesieve columns for routine air monitoring applications Molecular sieve columns have a tendency to adsorb water which, over time, results in changes in retention time. We use an advanced, proprietary deactivation process which allows for rapid regeneration. Fully saturated HP-PLOT Molesieve columns regenerate in 7 hours or less at 200 C. Test Mix: Test mixtures for PLOT columns are specially produced mixtures of light gases. HP-PLOT Molesieve Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) Thin Film to P-MS to P-MS to P-MS to P-MS6 504 Thick Film to P-MS to P-MS to P-MS C is the lowest temperature used on this phase in our lab. Lower temperatures may be used depending on the sample. Similar Phases: Molesieve 5Å, PLT-5A, Rt-Msieve 13X Please contact Technical Support for the test mix specifications and manufacturer's contact information. GS-Q Porous divinylbenzene homopolymer A PLOT column with polarity between Porapak-Q and Porapak-N A PLOT column to replace packed gas-solid columns Separates ethane, ethylene and ethyne (acetylene) Improved resolution in less time than conventional packed columns Minimal conditioning time required 1 hour GS-Q has been proven useful for separations of various gases including Freons and sulfides (except for SO 2 ). Although GS-Q is not suitable for quantitating water or alcohols, aqueous injections will not harm the column. GS-Q Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) N/A -60 to N/A -60 to N/A -60 to N/A -60 to H N/A -60 to N/A -60 to N/A -60 to C is the lowest temperature used on this phase in our lab. Lower temperatures may be used depending on the sample. Test Mix: Test mixtures for PLOT columns are specially produced mixtures of light gases. Please contact Technical Support for the test mix specifications and manufacturer's contact information. Similar Phases: CP PoraPLOT Q, CP PoraPLOT Q-HT, Rt-QPLOT, SupelQ PLOT, HP-Q PLOT 46 still made by elves v pricedfinal-ap.p /21/01, 9:42 AM

48 Phase Specifications and Order Guides SE Series GC Columns SE-30: 100% Dimethylpolysiloxane SE-54: (5%-Phenyl)(1%-Vinyl)-methylpolysiloxane Because SE series GC columns are not bonded or cross-linked, we do not recommend solvent rinsing. Test Mix: SE-54 P/N: Certain thick film or thin film configurations require different test mixtures than that listed above. Please call Technical Support for specific test mix information. SE-30 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 325/ to 325/ to 325/ DB-1 is a bonded replacement for SE-30. SE-54 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 325/ to 325/ to 325/ to 325/ DB-5 is a bonded replacement for SE-54. J&W Dual Columns Specific configurations for pesticides, PCBs and volatile priority pollutants No assembly required! Includes two columns, three-way union and 10 meter 0.53 mm I.D. guard column Matched column dimensions for even sample splitting Leak-free Y connections Performance tested Meets U.S. EPA Method requirements Bonded and cross-linked DB-5/1701P Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price Phase (mm I.D.) (meter) (µm) ( C) (P/N) ($) DB-5 and to 280/ ,131 DB-1701P DB-17/1701P Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price Phase (mm I.D.) (meter) (µm) ( C) (P/N) ($) DB-17 and to 280/ ,131 DB-1701P DB-5/17 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price Phase (mm I.D.) (meter) (µm) ( C) (P/N) ($) DB-5 and to 280/ ,131 DB v pricedfinal-ap.p /21/01, 9:42 AM

49 Phase Specifications and Order Guides DB-ProSteel TM Series Stainless Steel GC Columns Excellent inertness Virtually unbreakable Available in a wide variety of stationary phases Bonded and cross-linked Our easy to handle DB-ProSteel metal columns are deactivated with a new secret sauce (this is not glass lined steel) to provide inertness which truly rivals fused silica. J&W's DB-ProSteel metal columns can be custom wound upon request for small GC ovens. Several of our most popular bonded phases are available in metal. Looking for a metal column that is not listed? We are developing other dimensions and phases. Give us a call with your suggestions and/or requirements. DB-ProSteel GC columns have the same outer diameter as standard Megabore (0.53 mm I.D.), so no special ferrules are required. DB-ProSteel Chromatograms Alcohols (DB-PS1 and DB-PSWAX) page 88 Acrylate Impurities (DB-PS1701) page 90 Contact the Custom Column Shop (page 7) for additional ProSteel columns and phases. DB-PS1 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 300/ to 300/ to 300/ to 300/ to 300/ to 300/ to 260/ to 260/ DB-PS5 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 300/ DB-PS624 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to DB-PS2887 Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to DB-PSWAX Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 230/ to 230/ DB-PSHT Sim Dis Order Guide Inner Film Temperature Part Diameter Length Thickness Limits Number Price (mm I.D.) (meter) (µm) ( C) (P/N) ($) to 400/ ProSteel Stainless Steel, Deactivated I.D. (mm) O.D. (mm) Length (m) Part Number (P/N) Price ($ v pricedfinal-ap.p /13/01, 9:30 PM

50 Solvent Retention Data DB Alphabetical Order Column: P/N: Oven: Carrier: DB m x 0.53 mm I.D., 3 µm 40 C for 5 min C at 10 /min 260 C for 3 min Helium, constant pressure at 30 cm/sec (40 C) Injector: Split 1:10, 250 C Detector: FID, 300 C Retention Time Compound acetal (acetaldehyde diethyl acetal) 2.46 acetaldeyde 9.10 acetic acid 4.05 acetone 4.27 acetonitrile acetophenone 3.81 acrolein acrylic acid 5.22 acrylonitrile 9.65 allyl ether 6.41 allyl ethyl ether amyl acetate benzaldehyde 8.69 benzene benzonitrile benzyl acetate benzyl alcohol benzyl ether bromobenzene 7.59 bromochloromethane bromodichloromethane 4.27 bromoethane bromoform ,3-butanediol ,4-butanediol ,3-butanediol ,3-butanedione (diacetal) butanol butanone (MEK) buten-1-ol (crotyl alcohol) buten-1-ol butoxyethanol (butyl cellosolve) butyl acetate butylbenzene butyl ether 9.34 butyl ethyl ether 7.10 butyl methyl ether 6.84 butyraldehyde 4.27 carbon disulfide 8.34 carbon tetrachloride chlorobenzene chlorobutane chlorodibromomethane 7.75 chloroform chlorohexane chloro-4-nitrobenzene chloropropene (allyl chloride) chlorostyrene chlorotoluene chlorotoluene chlorotoluene 9.18 crotonaldehyde 8.10 cyclohexane cyclohexanol cyclohexanone cyclopentanol cyclopentanone decane ,10-decanediol decanol ,2-dibromo-3-chloropropane (DBCP) ,2-dibromoethane (EDB) dibromomethane ,2-dichlorobenzene ,3-dichlorobenzene ,4-dichlorobenzene trans-1,4-dichloro-2-butene ,1-dichloroethane ,2-dichloroethane (ethylene dichloride) ,1-dichloroethylene (vinylidine chloride) 7.16 cis-1,2-dichloroethylene 5.33 trans-1,2-dichloroethylene ,1-dichloropropane ,2-dichloropropane ,3-dichloropropane ,2-dichloropropane ,1-dichloropropene cis-1,3-dichloropropene trans-1,3-dichloropropene Retention Time Compound diethylene glycol diethylene glycol monobutyl ether diethylene glycol monoethyl ether diethylene glycol monomethyl ether 9.92 diglyme (diethylene glycol dimethyl ether) ,3-diisopropylbenzene ,4-diisopropylbenzene ,4-dimethyl-3-pentanone (diisopropyl ketone) ,6-dimethyl-4-heptanone (diisobutyl ketone) ,4-dioxane ,3-dioxolane DMF (dimethylformamide) DMSO (methyl sulfoxide) dodecane epichlorohydrin 3.47 ethanol ethoxyethanol (cellosolve) ethoxyethyl acetate 7.34 ethyl acetate ethyl acrylate ethylbenzene ethyl benzoate ethylene glycol ethylene glycol monobutyl ether ethylene glycol monoethyl ether 8.64 ethylene glycol monomethyl ether 3.72 ethyl ether 4.27 ethyl formate ethyl-1-hexanol α-ethylphenethyl alcohol β-ethylphenethyl alcohol ethyl propionate 3.72 ethyl vinyl ether 9.18 fluorobenzene 3.24 fluorotrichloromethane (Freon 11) 3.72 furan furfural furfuryl alcohol glycidol 8.65 glyme (propylene glycol dimethyl ether) heptanal 9.15 heptane ,7-heptanediol heptanol heptanol heptanol heptanone heptanone heptanone trans-2-hepten-1-ol cis-4-hepten-1-ol hexachloro-1,3-butadiene hexadecane hexanal 5.82 hexane ,6-hexanediol hexanol hexanol hexanol hexanone hexanone cis-2-hexen-1-ol cis-3-hexen-1-ol hexen-3-one hydroxy-4-methyl-2-pentanone iodobenzene 4.27 iodomethane iso-amyl acetate 8.60 iso-butanol iso-butyl acetate iso-butylbenzene 5.66 iso-butyraldehyde 8.81 iso-octane isophorone 4.27 iso-propanol 8.87 iso-propyl acetate iso-propylbenzene (cumene) 6.23 iso-propyl ether 6.01 methacrolein 7.53 methacrylonitrile 2.59 methanol methoxyethanol (methyl cellosolve) 4.60 methyl acetate methyl benzoate methyl-1-butanol (active amyl alcohol) methyl-2-butanol (tert-amyl alcohol) methyl-1-butanol (iso-amyl alcohol) methyl-2-butanone methyl-3-buten-2-ol methyl-2-buten-1-ol methylbutyl acetate 4.80 methylene chloride 2.80 methyl formate methyl-3-heptanone methyl-2-hexanone methyl-3-pentanone methyl-2-pentanone (MIBK; methyl isobutyl ketone) Retention Time Compound methyl-2-pentanol methyl-3-penten-3-one α-methylphenyl alcohol 7.88 methyl propionate methylpyrrolidone α-methylstyrene methylstyrene 5.30 methyl tert-butyl ether (MTBE) morpholine nitrobenzene nitrotoluene nitrotoluene nitrotoluene nonanal nonane ,9-nonanediol nonanol octanal octane ,8-octanediol octanol octanone octanone pentachlorobenzene pentadecane pentanal (valeraldehyde) 3.37 pentane ,5-pentanediol ,3-pentanedione pentanol pentanol pentanol pentanone pentanone penten-3-ol penten-1-ol penten-3-one (ethyl vinyl ketone) penten-2-one (methyl vinyl ketone) pentyl ether phenoxyethanol phenyl-2-butanone (benzyl acetone) ,3-propanediol propanol propen-1-ol (allyl alcohol) 3.91 propionaldehyde propionic acid 7.25 propionitrile propyl acetate propylbenzene propyl benzoate propylene glycol (1,2-propanediol) 9.05 propyl ether 7.66 propyl formate propyl propionate propyn-1-ol (propargyl alcohol) pyridine 7.55 sec-butanol sec-butyl acetate sec-butylbenzene styrene styrene oxide 8.89 tert-amyl methyl ether 5.01 tert-butanol tert-butyl acetate tert-butylbenzene 6.90 tert-butyl ethyl ether tert-butyltoluene ,2,4,5-tetrachlorobenzene ,1,1,2-tetrachloroethane ,1,2,2-tetrachloroethane tetrachloroethylene tetradecane tetrahydropyran 7.64 THF (tetrahydrofuran) m-tolualdehyde o-tolualdehyde p-tolualdehyde toluene ,2,3-trichlorobenzene ,2,4-trichlorobenzene ,3,5-trichlorobenzene ,1,1-trichloroethane ,1,2-trichloroethane 9.80 trichloroethylene ,2,3-trichloropropane ,1,2-trichlorotrifluoroethane (Freon 113) tridecane 8.91 triethylamine triglyme (triethylene glycol dimethyl ether) ,2,3-trimethylbenzene (hemimellitene) ,2,4-trimethylbenzene (pseudocumene) ,3,5-trimethylbenzene (mesitylene) undecane 6.37 vinyl acetate m-xylene o-xylene p-xylene 49 v priced-ap.p /13/01, 10:01 PM

51 Solvent Retention Data DB Retention Order Column: P/N: Oven: Carrier: DB m x 0.53 mm I.D., 3 µm 40 C for 5 min C at 10 /min 260 C for 3 min Helium, constant pressure at 30 cm/sec (40 C) Injector: Split 1:10, 250 C Detector: FID, 300 C Retention Time Compound 2.46 acetaldeyde 2.59 methanol 2.80 methyl formate 3.24 fluorotrichloromethane (Freon 11) 3.37 pentane 3.47 ethanol 3.72 ethyl ether 3.72 ethyl vinyl ether 3.72 furan 3.81 acrolein 3.91 propionaldehyde ,1-dichloroethylene (vinylidine chloride) ,1,2-trichlorotrifluoroethane (Freon 113) 4.05 acetone 4.27 acetonitrile 4.27 bromoethane 4.27 carbon disulfide 4.27 ethyl formate 4.27 iodomethane 4.27 iso-propanol chloropropene (allyl chloride) 4.60 methyl acetate 4.80 methylene chloride 5.01 tert-butanol 5.22 acrylonitrile 5.30 methyl tert-butyl ether (MTBE) 5.33 trans-1,2-dichloroethylene 5.66 iso-butyraldehyde 5.82 hexane 6.01 methacrolein ,1-dichloroethane propen-1-ol (allyl alcohol) 6.23 iso-propyl ether propanol 6.37 vinyl acetate 6.41 allyl ethyl ether 6.84 butyraldehyde 6.90 tert-butyl ethyl ether penten-2-one (methyl vinyl ketone) ,3-butanedione (diacetal) 7.10 butyl methyl ether 7.16 cis-1,2-dichloroethylene ,2-dichloropropane butanone (MEK) 7.25 propionitrile ,3-dioxolane 7.34 ethyl acetate 7.53 methacrylonitrile 7.55 sec-butanol propyn-1-ol (propargyl alcohol) 7.59 bromochloromethane 7.64 THF (tetrahydrofuran) 7.66 propyl formate 7.75 chloroform 7.88 methyl propionate methyl-3-buten-2-ol ,1,1-trichloroethane 8.10 cyclohexane chlorobutane 8.34 carbon tetrachloride ,1-dichloropropene 8.60 iso-butanol 8.64 ethylene glycol monomethyl ether 8.65 glyme (propylene glycol dimethyl ether) methoxyethanol (methyl cellosolve) 8.69 benzene ,1-dichloropropane methyl-2-butanol (tert-amyl alcohol) 8.81 iso-octane 8.87 iso-propyl acetate 8.89 tert-amyl methyl ether 8.91 triethylamine buten-1-ol 9.05 propyl ether 9.10 acetic acid 9.15 heptane 9.18 crotonaldehyde 9.18 fluorobenzene methyl-2-butanone 9.34 butyl ethyl ether Retention Time Compound ,2-dichloroethane (ethylene dichloride) 9.65 allyl ether butanol 9.80 trichloroethylene buten-1-ol (crotyl alcohol) 9.92 diglyme (diethylene glycol dimethyl ether) tert-butyl acetate ethyl acrylate penten-3-one (ethyl vinyl ketone) tetrahydropyran pentanone ,2-dichloropropane penten-3-ol pentanal (valeraldehyde) ,3-pentanedione pentanone dibromomethane ethyl propionate ,4-dioxane propyl acetate acetal (acetaldehyde diethyl acetal) pentanol pentanol bromodichloromethane ethylene glycol monoethyl ether ethoxyethanol (cellosolve) epichlorohydrin cis-1,3-dichloropropene methyl-2-pentanone (MIBK; methyl isobutyl ketone) pyridine sec-butyl acetate methyl-1-butanol (iso-amyl alcohol) methyl-3-pentanone methyl-1-butanol (active amyl alcohol) propionic acid glycidol toluene octane ethylene glycol iso-butyl acetate acrylic acid methyl-2-pentanol trans-1,3-dichloropropene ,1,2-trichloroethane pentanol penten-1-ol hexanone methyl-2-buten-1-ol ,3-dichloropropane tetrachloroethylene ,4-dimethyl-3-pentanone (diisopropyl ketone) morpholine hexanone propyl propionate ,3-propanediol hexanal cyclopentanol hexanol propylene glycol (1,2-propanediol) methyl-3-penten-3-one butyl acetate chlorodibromomethane hexanol cyclopentanone ,2-dibromoethane (EDB) DMF (dimethylformamide) ,3-butanediol chlorohexane hexen-3-one chlorobenzene ,1,1,2-tetrachloroethane butyl ether ethylbenzene m-xylene p-xylene furfural nonane methyl-2-hexanone iso-amyl acetate methylbutyl acetate cis-3-hexen-1-ol heptanone hydroxy-4-methyl-2-pentanone hexanol cis-2-hexen-1-ol styrene o-xylene heptanone furfuryl alcohol heptanone heptanol amyl acetate ethoxyethyl acetate DMSO (methyl sulfoxide) heptanal bromoform cyclohexanol heptanol Retention Time Compound ethylene glycol monobutyl ether iso-propylbenzene (cumene) butoxyethanol (butyl cellosolve) cyclohexanone ,3-butanediol ,1,2,2-tetrachloroethane bromobenzene trans-1,4-dichloro-2-butene ,2,3-trichloropropane methyl-3-heptanone propylbenzene chlorotoluene diethylene glycol monomethyl ether chlorotoluene chlorotoluene decane ,3,5-trimethylbenzene (mesitylene) ,6-dimethyl-4-heptanone (diisobutyl ketone) heptanol trans-2-hepten-1-ol cis-4-hepten-1-ol α-methylstyrene tert-butylbenzene benzaldehyde octanone ,2,4-trimethylbenzene (pseudocumene) octanone iso-butylbenzene sec-butylbenzene methylstyrene ,4-butanediol octanal ,3-dichlorobenzene diethylene glycol monoethyl ether iodobenzene ,2,3-trimethylbenzene (hemimellitene) benzonitrile diethylene glycol ethyl-1-hexanol pentyl ether butylbenzene ,2-dichlorobenzene undecane ,4-dichlorobenzene octanol benzyl alcohol tert-butyltoluene chlorostyrene styrene oxide ,5-pentanediol α-methylphenyl alcohol m-tolualdehyde o-tolualdehyde methyl-pyrrolidone acetophenone nonanal methyl benzoate p-tolualdehyde ,2-dibromo-3-chloropropane (DBCP) ,3-diisopropylbenzene nitrobenzene ,3,5-trichlorobenzene dodecane ,4-diisopropylbenzene nonanol isophorone benzyl acetate ethyl benzoate ,6-hexanediol ,2,4-trichlorobenzene diethylene glycol monobutyl ether nitrotoluene hexachloro-1,3-butadiene triglyme (triethylene glycol dimethyl ether) ,2,3-trichlorobenzene tridecane nitrotoluene decanol α-ethylphenethyl alcohol phenoxyethanol nitrotoluene phenyl-2-butanone (benzyl acetone) propyl benzoate ,7-heptanediol β-ethylphenethyl alcohol chloro-4-nitrobenzene tetradecane ,2,4,5-tetrachlorobenzene ,8-octanediol pentadecane ,9-nonanediol hexadecane pentachlorobenzene ,10-decanediol benzyl ether 50 v priced-ap.p /14/01, 7:57 PM

52 Solvent Retention Data DB-1 - Alphabetical Order Column: DB-1 30 m x 0.53 mm I.D., 3 µm J&W P/N: Oven: 40 C for 5 min Carrier: C at 10 /min Helium, constant pressure at 30 cm/sec (40 C) Injector: Split 1:10, 250 C Detector: FID, 300 C Retention Time Compound acetal (acetaldehyde diethyl acetal) 2.16 acetaldehyde acetic acid 3.05 acetone 2.87 acetonitrile acetophenone 2.98 acrolein acrylic acid 3.43 acrylonitrile 9.05 allyl ether 6.00 allyl ethyl ether amyl acetate benzaldehyde 8.00 benzene benzonitrile benzyl acetate benzyl alcohol benzyl ether bromobenzene 4.79 bromochloromethane 9.22 bromodichloromethane 3.75 bromoethane bromoform ,3-butanediol ,4-butanediol ,3-butanediol ,3-butanedione (diacetal) butanol butanone (MEK; methyl ethyl ketone) buten-1-ol (crotyl alcohol) buten-1-ol butoxyethanol (butyl cellosolve) butyl acetate butyl ether 9.18 butyl ethyl ether 6.85 butyl methyl ether butylbenzene 5.29 butyraldehyde 4.37 iso-butyraldehyde 4.09 carbon disulfide 8.18 carbon tetrachloride chlorobenzene chlorobutane chlorodibromomethane 6.34 chloroform chlorohexane chloro-4-nitrobenzene chloropropene (allyl chloride) chlorostyrene chlorotoluene chlorotoluene chlorotoluene 7.03 crotonaldehyde 8.32 cyclohexane cyclohexanol cyclohexanone cyclopentanol cyclopentanone ,4-dimethyl-3-pentanone (diisopropyl ketone) ,6-dimethyl-4-heptanone (diisobutyl ketone) decane ,10-decanediol decanol ,2-dibromo-3-chloropropane (DBCP) ,2-dibromoethane (EDB) 8.93 dibromomethane ,2-dichlorobenzene ,3-dichlorobenzene ,4-dichlorobenzene trans-1,4-dichloro-2-butene ,1-dichloroethane ,2-dichloroethane (ethylene dichloride) ,1-dichloroethylene (vinylidine chloride) 5.98 cis-1,2-dichloroethylene 6.17 trans-1,2-dichloroethylene ,1-dichloropropane ,2-dichloropropane ,3-dichloropropane ,2-dichloropropane ,1-dichloropropene cis-1,3-dichloropropene trans-1,3-dichloropropene Retention Time Compound diethylene glycol diethylene glycol monobutyl ether diethylene monoethyl ether diethylene monomethyl ether 8.68 diglyme (diethylene glycol dimethyl ether) ,3-diisopropylbenzene ,4-diisopropylbenzene DMF (dimethylformamide) DMSO (methylsulfoxide) ,4-dioxane ,3-dioxolane dodecane 9.35 epichlorohydrin 2.68 ethanol ethoxyethanol (cellosolve) ethoxyethyl acetate 6.21 ethyl acetate 8.93 ethyl acrylate ethylbenzene ethyl benzoate 8.54 ethylene glycol ethylene glycol monobutyl ether 9.35 ethylene glycol monoethyl ether 6.72 ethylene glycol monomethyl ether 3.50 ethyl ether 3.56 ethyl formate ethyl-1-hexanol α-ethylphenethyl alcohol β-ethylphenethyl alcohol 9.42 ethyl propionate 3.50 ethyl vinyl ether 8.36 fluorobenzene 3.23 fluorotrichloromethane (Freon 11) 3.36 furan furfural furfuryl alcohol 9.10 glycidol 7.56 glyme (propylene glycol dimethyl ether) heptanal 9.58 heptane ,7-heptanediol heptanol heptanol heptanol heptanone heptanone heptanone trans-2-hepten-1-ol cis-4-hepten-1-ol hexachloro-1,3-butadiene hexadecane hexanal 6.25 hexane ,6-hexanediol hexanol hexanol hexanol hexanone hexanone cis-2-hexen-1-ol cis-3-hexen-1-ol hexen-3-one hydroxy-4-methyl-2-pentanone iodobenzene 3.75 iodomethane iso-amyl acetate 6.74 iso-butanol iso-butyl acetate iso-butylbenzene 9.27 iso-octane isophorone 3.22 iso-propanol 7.88 iso-propyl acetate 6.21 iso-propyl ether iso-propylbenzene (cumene) 4.68 methacrolein 5.36 methacrylonitrile 2.15 methanol methoxyethanol (methyl cellosolve) 3.79 methyl acetate methyl benzoate methyl-1-butanol (active amyl alcohol) methyl-2-butanol (tert-amyl alcohol) methyl-1-butanol (iso-amyl alcohol) methyl-2-butanone methyl-3-buten-2-ol methyl-2-buten-1-ol methylbutyl acetate 3.85 methylene chloride 2.44 methyl formate methyl-3-heptanone methyl-2-hexanone methyl-3-pentanone methyl-2-pentanone (MIBK; methyl isobutyl ketone) methyl-2-pentanol methyl-3-penten-3-one α-methylphenyl alcohol 6.78 methyl propionate Retention Time Compound methyl-2-pyrrolidone α-methylstyrene methylstyrene 5.06 methyl tert-butyl ether (MTBE) morpholine nonanal nonane ,9-nonanediol nonanol nitrobenzene nitrotoluene nitrotoluene nitrotoluene octanal octane ,8-octanediol octanol octanone octanone pentachlorobenzene pentadecane 8.76 pentanal (valeraldehyde) 3.51 pentane ,5-pentanediol ,3-pentanedione pentanol pentanol pentanol pentanone pentanone penten-3-ol penten-1-ol penten-3-one (ethyl vinyl ketone) penten-2-one (methyl vinyl ketone) pentyl ether phenoxyethanol phenyl-2-butanone (benzyl acetone) ,3-propanediol propanol propen-1-ol (allyl alcohol) 3.11 propionaldehyde propionic acid 4.43 propionitrile propyn-1-ol (propargyl alcohol) 9.47 propyl acetate propylbenzene propyl benzoate 9.05 propyl ether 9.90 propylene glycol (1,2-propanediol) 6.48 propyl formate propyl propionate pyridine 5.80 sec-butanol sec-butyl acetate sec-butylbenzene styrene styrene oxide 8.68 tert-amyl methyl ether 3.72 tert-butanol 9.32 tert-butyl acetate tert-butylbenzene 6.85 tert-butyl ethyl ether tert-butyltoluene ,2,4,5-tetrachlorobenzene ,1,1,2-tetrachloroethane ,1,2,2-tetrachloroethane tetrachloroethylene tetradecane 9.35 tetrahydropyran 6.75 THF (tetrahydrofuran) m-tolualdehyde o-tolualdehyde p-tolualdehyde toluene ,2,3-trichlorobenzene ,2,4-trichlorobenzene ,3,5-trichlorobenzene ,1,1-trichloroethane ,1,2-trichloroethane 9.22 trichloroethylene ,2,3-trichloropropane ,1,2-trichlorotrifluoroethane (Freon 113) tridecane 8.93 triethylamine triglyme (triethylene glycol dimethyl ether) ,2,3-trimethylbenzene (hemimellitene) ,2,4-trimethylbenzene (pseudocumene) ,3,5-trimethylbenzene (mesitylene) undecane 4.09 vinyl acetate m-xylene o-xylene p-xylene 51 v priced-ap.p /13/01, 10:12 PM

53 Solvent Retention Data DB-1 - Retention Order Column: DB-1 30 m x 0.53 mm I.D., 3 µm P/N: Oven: 40 C for 5 min Carrier: C at 10 /min Helium, constant pressure at 30 cm/sec (40 C) Injector: Split 1:10, 250 C Detector: FID, 300 C Retention Time Compound 2.15 methanol 2.16 acetaldehyde 2.44 methyl formate 2.68 ethanol 2.87 acetonitrile 2.98 acrolein 3.05 acetone 3.11 propionaldehyde 3.22 iso-propanol 3.23 fluorotrichloromethane (Freon 11) 3.36 furan 3.43 acrylonitrile 3.50 ethyl ether 3.50 ethyl vinyl ether 3.51 n-pentane 3.56 ethyl formate 3.72 tert-butanol 3.75 bromoethane ,1-dichloroethylene (vinylidine chloride) 3.75 iodomethane 3.79 methyl acetate 3.85 methylene chloride chloropropene (allyl chloride) propen-1-ol (allyl alcohol) 4.09 carbon disulfide 4.09 vinyl acetate ,3-dioxolane ,1,2-trichlorotrifluoroethane (Freon 113) 4.37 iso-butyraldehyde 4.43 propionitrile propanol propyn-1-ol (propargyl alcohol) 4.68 methacrolein 4.79 bromochloromethane ,1-dichloroethane 5.06 methyl tert-butyl ether (MTBE) penten-2-one (methyl vinyl ketone) ,3-butanedione (diacetal) 5.29 butyraldehyde 5.36 methacrylonitrile butanone (MEK; methyl ethyl ketone) 5.80 sec-butanol 5.98 cis-1,2-dichloroethylene 6.00 allyl ethyl ether 6.17 trans-1,2-dichloroethylene methyl-3-buten-2-ol 6.21 ethyl acetate 6.21 iso-propyl ether 6.25 n-hexane 6.34 chloroform ,2-dichloropropane 6.48 propyl formate 6.72 ethylene glycol methyl ether 6.74 iso-butanol methoxyethanol (methyl cellosolve) 6.75 THF (tetrahydrofuran) 6.78 methyl propionate 6.85 tert-butyl ethyl ether 6.85 butyl methyl ether buten-1-ol 7.03 crotonaldehyde methyl-2-butanol (tert-amyl alcohol) ,2-dichloroethane (ethylene dichloride) ,1,1-trichloroethane chlorobutane 7.56 glyme (propylene glycol dimethyl ether) methyl-2-butanone ,1-dichloropropene 7.88 iso-propyl acetate butanol buten-1-ol (crotyl alcohol) 8.00 benzene 8.18 carbon tetrachloride 8.32 cyclohexane 8.36 fluorobenzene penten-3-one (ethyl vinyl ketone) pentanone 8.54 ethylene glycol penten-3-ol ,1-dichloropropane Retention Time Compound ,3-pentanedione 8.68 tert-amyl methyl ether 8.68 diglyme (diethylene glycol dimethyl ether) 8.76 pentanal (valeraldehyde) pentanone 8.93 ethyl acrylate ,2-dichloropropane 8.93 dibromomethane 8.93 triethylamine 9.05 allyl ether 9.05 propyl ether 9.10 glycidol pentanol pentanol ,4-dioxane 9.18 butyl ethyl ether 9.22 bromodichloromethane 9.22 trichloroethylene 9.27 iso-octane 9.32 tert-butyl acetate 9.35 epichlorohydrin 9.35 ethylene glycol ethyl ether 9.35 tetrahydropyran ethoxyethanol (cellosolve) 9.42 ethyl propionate 9.47 propyl acetate 9.58 n-heptane 9.90 propylene glycol (1,2-propanediol) ,3-propanediol methyl-1-butanol (iso-amyl alcohol) cis-1,3-dichloropropene pyridine acetal (acetaldehyde diethyl acetal) methyl-2-pentanone (MIBK; methyl isobutyl ketone) methyl-1-butanol (active amyl alcohol) methyl-3-pentanone trans-1,3-dichloropropene DMF (dimethylformamide) sec-butyl acetate methyl-2-pentanol ,1,2-trichloroethane pentanol penten-1-ol iso-butyl acetate methyl-2-buten-1-ol toluene ,3-dichloropropane ,3-butanediol cyclopentanone hexanone cyclopentanol hexanone chlorodibromomethane hexanal methyl-3-penten-3-one ,4-dimethyl-3-pentanone (diisopropyl ketone) DMSO (methyl sulfoxide) hexanol hexanol ,2-dibromoethane (EDB) propyl propionate butyl acetate n-octane furfural tetrachloroethylene hexen-3-one hydroxy-4-methyl-2-pentanone furfuryl alcohol ,3-butanediol ,1,1,2-tetrachloroethane chlorobenzene methyl-2-hexanone cis-3-hexen-1-ol morpholine chlorohexane hexanol cis-2-hexen-1-ol heptanone ethylbenzene iso-amyl acetate amyl acetate m-xylene p-xylene heptanone bromoform cyclohexanol cyclohexanone heptanone butyl ether ethoxyethyl acetate heptanal heptanol styrene heptanol ,1,2,2-tetrachloroethane ethylene glycol butyl ether o-xylene Retention Time Compound butoxyethanol (butyl cellosolve) methylbutyl acetate ,2,3-trichloropropane trans-1,4-dichloro-2-butene n-nonane ,4-butanediol diethylene glycol methyl ether iso-propylbenzene (cumene) bromobenzene methyl-3-heptanone diethylene glycol benzaldehyde cis-4-hepten-1-ol chlorotoluene chlorotoluene chlorotoluene propylbenzene trans-2-hepten-1-ol heptanol benzonitrile ,6-dimethyl-4-heptanone (diisobutyl ketone) ,3,5-trimethylbenzene (mesitylene) octanone octanone diethylene glycol ethyl ether α-methylstyrene octanal acetic acid tert-butylbenzene methylstyrene ,2,4-trimethylbenzene (pseudocumene) n-decane ,5-pentanediol ,3-dichlorobenzene methyl-2-pyrrolidone iso-butylbenzene ,4-chlorobenzene sec-butylbenzene benzyl alcohol ethyl-1-hexanol ,2,3-trimethylbenzene (hemimellitene) ,2-dichlorobenzene iodobenzene α-methylphenyl alcohol acetophenone propionic acid octanol m-tolualdehyde o-toluladehyde styrene oxide butylbenzene ,2-dibromo-3-chloropropane (DBCP) p-tolualdehyde pentyl ether chlorostyrene nitrobenzene methyl benzoate nononal tert-butyltoluene n-undecane ,6-hexanediol isophorone acrylic acid ,3,5-trichlorobenzene benzyl acetate ,3-diisopropylbenzene nitrotoluene nonanol ethyl benzoate diethylene glycol butyl ether ,4-diisopropylbenzene ,2,4-trichlorobenzene nitrotoluene triglyme (triethylene glycol dimethyl ether) n-dodecane phenoxyethanol ,7-heptanediol nitrotoluene ,2,3-trichlorobenzene chloro-4-nitrobenzene phenyl-2-butanone (benzyl acetone) α-ethylphenethyl alcohol hexachloro-1,3-butadiene β-ethylphenethyl alcohol decanol propyl benzoate n-tridecane ,8-octanediol ,2,4,5-tetrachlorobenzene n-tetradecane ,9-nonanediol n-pentadecane ,10-decanediol pentachlorobenzene n-hexadecane benzyl ether 52 v priced-ap.p /13/01, 10:14 PM

54 Solvent Retention Data DB-WAX - Alphabetical Order Column: P/N: Oven: Carrier: DB-WAX 30 m x 0.53 mm I.D., 1 µm 40 C for 5 min C at 10 /min 230 C for 7 min Helium, constant pressure at 34 cm/sec (40 C) Injector: Split 1:10, 250 C Detector: FID, 300 C Retention Time Compound 5.09 acetal (acetaldehyde diethyl acetal) 2.47 acetaldeyde acetic acid 3.60 acetone 8.12 acetonitrile acetophenone 4.10 acrolein acrylic acid 7.81 acrylonitrile 6.04 allyl ether 3.05 allyl ethyl ether amyl acetate benzaldehyde 6.46 benzene benzonitrile benzyl acetate benzyl alcohol benzyl ether bromobenzene 9.26 bromochloromethane bromodichloromethane 2.95 bromoethane bromoform ,3-butanediol ,4-butanediol ,3-butanediol ,3-butanedione (diacetal) butanol butanone (MEK; methyl ethyl ketone) buten-1-ol (crotyl alcohol) buten-1-ol butoxyethanol (butyl cellosolve) 9.85 butyl acetate butylbenzene 6.97 butyl ether 3.27 butyl ethyl ether 2.80 butyl methyl ether 4.72 butyraldehyde 2.65 carbon disulfide 4.85 carbon tetrachloride chlorobenzene chlorobutane chlorodibromomethane 8.58 chloroform chlorohexane chloro-4-nitrobenzene chloropropene (allyl chloride) chlorostyrene chlorotoluene chlorotoluene chlorotoluene 9.07 crotonaldehyde 2.27 cyclohexane cyclohexanol cyclohexanone cyclopentanol cyclopentanone 7.63 decane ,10-decanediol decanol ,2-dibromo-3-chloropropane (DBCP) ,2-dibromoethane (EDB) dibromomethane ,2-dichlorobenzene ,3-dichlorobenzene ,4-dichlorobenzene trans-1,4-dichloro-2-butene ,1-dichloroethane ,2-dichloroethane (ethylene dichloride) ,1-dichloroethylene (vinylidine chloride) 7.84 cis-1,2-dichloroethylene 4.38 trans-1,2-dichloroethylene ,1-dichloropropane ,2-dichloropropane ,3-dichloropropane ,2-dichloropropane ,1-dichloropropene cis-1,3-dichloropropene Retention Time Compound trans-1,3-dichloropropene diethylene glycol diethylene glycol monobutyl ether diethylene gycol monoethyl ether diethylene glycol monomethyl ether 6.04 diglyme (diethylene glycol dimethyl ether) ,3-diisopropylbenzene ,4-diisopropylbenzene ,4-dimethyl-3-pentanone (diisopropyl ketone) ,6-dimethyl-4-heptanone (diisobutyl ketone) ,4-dioxane ,3-dioxolane DMF (dimethylformamide) DMSO (methyl sulfoxide) dodecane epichlorohydrin 6.46 ethanol ethoxyethanol (cellosolve) ethoxyethyl acetate 5.03 ethyl acetate 7.87 ethyl acrylate ethylbenzene ethyl benzoate ethylene glycol ethylene glycol monobutyl ether ethylene glycol monoethyl ether ethylene glycol monomethyl ether 2.13 ethyl ether 3.78 ethyl formate ethyl-1-hexanol α-ethylphenethyl alcohol β-ethylphenethyl alcohol 6.93 ethyl propionate 2.39 ethyl vinyl ether 7.72 fluorobenzene 2.13 fluorotrichloromethane (Freon 11) 3.27 furan furfural furfuryl alcohol glycidol 6.04 glyme (propylene glycol dimethyl ether) heptanal 2.65 heptane ,7-heptanediol heptanol heptanol heptanol heptanone heptanone heptanone trans-2-hepten-1-ol cis-4-hepten-1-ol hexachloro-1,3-butadiene hexadecane hexanal ,6-hexanediol 2.05 hexane hexanol hexanol hexanol hexanone hexanone cis-2-hexen-1-ol cis-3-hexen-1-ol hexen-3-one hydroxy-4-methyl-2-pentanone iodobenzene 3.46 iodomethane iso-amyl acetate iso-butanol 8.36 iso-butyl acetate iso-butylbenzene 3.54 iso-butyraldehyde 2.44 iso-octane isophorone 6.28 iso-propanol 5.32 iso-propyl acetate iso-propylbenzene (cumene) 3.27 iso-propyl ether 4.83 methacrolein 7.54 methacrylonitrile 5.40 methanol methoxyethanol (methyl cellosolve) 3.78 methyl acetate methyl benzoate methyl-1-butanol (active amyl alcohol) methyl-2-butanol (tert-amyl alcohol) methyl-1-butanol (iso-amyl alcohol) methyl-2-butanone methyl-3-buten-2-ol methyl-2-buten-1-ol methylbutyl acetate 6.18 methylene chloride 2.85 methyl formate methyl-3-heptanone methyl-2-hexanone methyl-3-pentanone methyl-2-pentanone (MIBK; methyl isobutyl ketone) Retention Time Compound methyl-2-pentanol methyl-3-penten-3-one α-methylphenyl alcohol 5.54 methyl propionate α-methylstyrene methylstyrene methyl-2-pyrrolidone 2.30 methyl tert-butyl ether (MTBE) morpholine nitrobenzene nitrotoluene nitrotoluene nitrotoluene nonanal 4.97 nonane ,9-nonanediol nonanol octanal 3.22 octane ,8-octanediol octanol octanone octanone pentachlorobenzene pentadecane 7.46 pentanal (valerlaldehyde) 1.89 pentane ,5-pentanediol ,3-pentanedione pentanol pentanol pentanol pentanone pentanone penten-3-ol penten-1-ol penten-3-one (ethyl vinyl ketone) penten-2-one (methyl vinyl ketone) pentyl ether phenoxyethanol phenyl-2-butanone (benzyl acetone) ,3-propanediol propanol propen-1-ol (allyl alcohol) 3.25 propionaldehyde propionic acid 8.72 propionitrile 7.38 propyl acetate propylbenzene propyl benzoate propylene glycol (1,2-propanediol) 3.05 propyl ether 5.93 propyl formate 9.17 propyl propionate propyn-1-ol (propargyl alcohol) pyridine 8.77 sec-butanol 7.69 sec-butyl acetate sec-butylbenzene styrene styrene oxide 3.27 tert-amyl methyl ether 5.54 tert-butanol 5.40 tert-butyl acetate tert-butylbenzene 2.47 tert-butyl ethyl ether tert-butyltoluene ,2,4,5-tetrachlorobenzene ,1,1,2-tetrachloroethane ,1,2,2-tetrachloroethane 8.58 tetrachloroethylene tetradecane 5.83 tetrahydropyran 4.45 THF (tetrahydrofuran) m-tolualdehyde o-tolualdehyde p-tolualdehyde 9.06 toluene ,2,3-trichlorobenzene ,2,4-trichlorobenzene ,3,5-trichlorobenzene ,1,1-trichloroethane ,1,2-trichloroethane 7.84 trichloroethylene ,2,3-trichloropropane ,1,2-trichlorotrifluoroethane (Freon 113) tridecane 3.26 triethylamine triglyme (triethylene glycol dimethyl ether) ,2,3-trimethylbenzene (hemimellitene) ,2,4-trimethylbenzene (pseudocumene) ,3,5-trimethylbenzene (mesitylene) undecane 5.03 vinyl acetate m-xylene o-xylene p-xylene 53 v priced-ap.p /14/01, 3:15 PM

55 Solvent Retention Data DB-WAX - Retention Order Column: P/N: Oven: Carrier: DB-WAX 30 m x 0.53 mm I.D., 1 µm 40 C for 5 min C at 10 /min 230 C for 7 min Helium, constant pressure at 34 cm/sec (40 C) Injector: Split 1:10, 250 C Detector: FID, 300 C Retention Time Compound 1.89 pentane 2.05 hexane ,1,2-trichlorotrifluoroethane (Freon 113) 2.13 fluorotrichloromethane (Freon 11) 2.13 ethyl ether 2.27 cyclohexane 2.30 methyl tert-butyl ether (MTBE) 2.39 ethyl vinyl ether 2.44 iso-octane 2.47 acetaldehyde 2.47 tert-butyl ethyl ether 2.65 carbon disulfide ,1-dichloroethylene (vinylidine chloride) 2.65 heptane 2.80 butyl methyl ether 2.85 methyl formate 2.95 bromoethane 3.05 allyl ethyl ether 3.05 propyl ether 3.22 octane 3.25 propionaldehyde 3.26 triethylamine 3.27 butyl ethyl ether 3.27 furan 3.27 tert-amyl methyl ether 3.27 iso-propyl ether chloropropene (allyl chloride) 3.46 iodomethane 3.54 iso-butyraldehyde 3.60 acetone 3.78 ethyl formate 3.78 methyl acetate chlorobutane ,2-dichloropropane 4.10 acrolein 4.38 trans-1,2-dichloroethylene 4.45 THF (tetrahydrofuran) ,1-dichloropropene 4.72 butyraldehyde 4.83 methacrolein 4.85 carbon tetrachloride ,1,1-trichloroethane ,2-dichloropropane 4.97 nonane 5.03 ethyl acetate 5.03 vinyl acetate 5.09 acetal (acetaldehyde diethyl acetal) 5.32 iso-propyl acetate butanone (MEK; methyl ethyl ketone) 5.40 tert-butyl acetate 5.40 methanol 5.54 tert-butanol 5.54 methyl propionate 5.83 tetrahydropyran 5.93 propyl formate 6.04 allyl ether 6.04 diglyme (diethylene glycol dimethyl ether) 6.04 glyme (propylene glycol dimethyl ether) methyl-2-butanone 6.18 methylene chloride 6.28 iso-propanol 6.46 benzene 6.46 ethanol penten-2-one (methyl vinyl ketone) 6.93 ethyl propionate 6.97 butyl ether ,3-dioxolane ,2-dichloroethane (ethylene dichloride) 7.38 propyl acetate ,3-butanedione (diacetal) pentanone pentanone 7.46 valeraldehyde 7.54 methacrylonitrile 7.63 decane 7.69 sec-butyl acetate 7.72 fluorobenzene 7.81 acrylonitrile 7.84 cis-1,2-dichloroethylene Retention Time Compound 7.84 trichloroethylene 7.87 ethyl acrylate ,4-dimethyl-3-pentanone (diisopropyl ketone) methyl-3-pentanone 8.12 acetonitrile methyl-2-pentanone (MIBK; methyl isobutyl ketone) 8.36 iso-butyl acetate methyl-2-butanol (tert-amyl alcohol) penten-3-one (ethyl vinyl ketone) 8.58 chloroform 8.58 tetrachloroethylene 8.72 propionitrile 8.77 sec-butanol 9.06 toluene 9.07 crotonaldehyde ,1-dichloroethane chlorohexane methyl-3-buten-2-ol propanol 9.17 propyl propionate 9.26 bromochloromethane hexanone ,3-pentanedione ,4-dioxane ,1-dichloropropane 9.85 butyl acetate hexanone hexanal undecane iso-butanol pentanol propen-1-ol (allyl alcohol) pentanol amyl acetate iso-amyl acetate heptanone ethylbenzene methyl-3-penten-3-one cis-1,3-dichloropropene p-xylene methyl-2-hexanone m-xylene butanol heptanone epichlorohydrin bromodichloromethane penten-3-ol methyl-2-pentanol dibromomethane buten-1-ol ,6-dimethyl-4-heptanone (diisobutyl ketone) methylbutyl acetate iso-propylbenzene (cumene) dodecane ethylene glycol monomethyl ether methoxyethanol (methyl cellosolve) heptanone heptanal methyl-3-heptanone ,3-dichloropropane o-xylene pyridine cyclopentanone hexanol hexen-3-one pentyl ether methyl-1-butanol (active amyl alcohol) methyl-1-butanol (iso-amyl alcohol) trans-1,3-dichloropropene propylbenzene buten-1-ol (crotyl alcohol) hexanol chlorobenzene ethylene glycol monoethyl ether ethoxyethanol (cellosolve) tert-butylbenzene iso-butylbenzene pentanol ,3,5-trimethylbenzene (mesitylene) morpholine sec-butylbenzene octanone ,1,1,2-tetrachloroethane ,1,2-trichloroethane ,2-dibromoethane (EDB) styrene tridecane octanone ,2,4-trimethylbenzene (pseudocumene) heptanol octanal ethoxyethyl acetate chlorodibromomethane cyclopentanol cyclohexanone penten-1-ol Retention Time Compound chlorotoluene heptanol butylbenzene methyl-2-buten-1-ol chlorotoluene chlorotoluene tert-butyltoluene α-methylstyrene propyn-1-ol (propargyl alcohol) DMF (dimethylformamide) ,2,3-trimethylbenzene (hemimellitene) hexanol ,3-diisopropylbenzene bromobenzene methylstyrene hydroxy-4-methyl-2-pentanone tetradecane cis-3-hexen-1-ol nononal ,4-diisopropylbenzene ethylene glycol monobutyl ether butoxyethanol (butyl cellosolve) cyclohexanol cis-2-hexen-1-ol ,3-dichlorobenzene acetic acid heptanol bromoform trans-1,4-dichloro-2-butene ,4-dichlorobenzene ,2,3-trichloropropane benzaldehyde glycidol pentadecane ethyl-1-hexanol cis-4-hepten-1-ol ,1,2,2-tetrachloroethane trans-2-hepten-1-ol ,2-dichlorobenzene hexachloro-1,3-butadiene propionic acid ,3,5-trichlorobenzene chlorostyrene iodobenzene furfural octanol ,3-butanediol hexadecane propylene glycol ,3-propanediol diethylene glycol monomethyl ether DMSO (methyl sulfoxide) styrene oxide isophorone ethylene glycol diethylene glycol monoethyl ether benzonitrile acrylic acid methyl benzoate o-toluladehyde m-tolualdehyde nonanol furfuryl alcohol ,2,4-trichlorobenzene p-tolualdehyde acetophenone ethyl benzoate ,2-dibromo-3-chloropropane (DBCP) methyl-2-pyrrolidone triglyme (triethylene glycol dimethyl ether) ,3-butanediol benzyl acetate decanol ,2,3-trichlorobenzene nitrobenzene propyl benzoate diethylene glycol monobutyl ether nitrotoluene α-methylphenyl alcohol ,2,4,5-tetrachlorobenzene nitrotoluene phenyl-2-butanone (benzyl acetone) benzyl alcohol α-ethylphenethyl alcohol ,4-butanediol nitrotoluene diethylene glycol chloro-4-nitrobenzene β-ethylphenethyl alcohol ,5-pentanediol pentachlorobenzene ,6-hexanediol phenoxyethanol ,7-heptanediol ,8-octanediol benzyl ether ,9-nonanediol ,10-decanediol 54 v priced-ap.p /13/01, 10:17 PM

56 Chromatograms Table of Contents Environmental Aroclors EPA Method Explosives Freons Haloacetic Acid Methyl Esters MTBE Organochlorine Pesticides Organophosphorous Pesticides PCB Congeners Phenoxy Acid Methyl Esters Phthalates Semivolatiles Volatiles ,60-63 Environmental Food, Flavors, Life Science Blood Alcohols Chiral Drugs FAMEs Fragrance Oils Fragrance Standard Organic Acids Solvents Sugars Triglycerides Petroleum & Industrial Chemicals Acrylate Impurities Alcohols Amines Gasoline High Temperature Simulated Distillation Hydrocarbons... 93,97-99 Organic Acids...90,92 Permanent Gases Solvents Sulfur Compounds v priced-ap.p /13/01, 10:27 PM

57 Environmental Methyl-tert-butyl Ether (MTBE), Extended 8020 Analysis Column: DB-MTBE 30 m x 0.45 mm I.D., 2.55 µm P/N: Carrier: Helium at 10 ml/min Oven: 35 C for 4 min C at 20 /min 200 C for 5 min Injector: Purge and trap (O.I.A. 4560) J&W LVI (Low Volume Injector), 150 C 40 ppb per component in 5 ml water Trap: Tenax TM only Preheat: 175 C Desorb: 180 C for 3 min Detector: FID (O.I.A. 4410), 200 C C Methyl-tert-butyl ether (MTBE) 2. 2-Methylpentane 3. 3-Methylpentane 4. Diisopropyl ether (DIPE) 5. Ethyl-tert-butyl ether (ETBE) 6. Benzene 7. tert-amyl methyl ether (TAME) 8. α,α,α-trifluorotoluene (SS) 9. Toluene 10. Ethylbenzene 11. m-xylene 12. p-xylene 13. o-xylene 14. Cumene 15. 1,3,5-Trimethylbenzene 16. 1,2,4-Trimethylbenzene 17. 1,2,3-Trimethylbenzene 18. Naphthalene SS - Surrogate Standard , C 1 and C 2 Halocarbons (Freons ) Column: GS-GasPro 60 m x 0.32 mm I.D. P/N: Carrier: Helium at 35 cm/sec constant velocity Oven: 40 C for 2 min, C at 10 /min 120 C for 3 min C at 10 /m n Injecto Splitless, min purge activation time, 1.0 µl of 100 ppm mixture of Accustandard M-REF & M-REF-X in methanol Detector: MSD, 280 C, full scan amu Compound Freon # 1. Chlorotrifluoromethane (peak not shown) Trifluoromethane Bromotrifluoromethane 13B1 4. Chloropentafluoroethane Pentafluoroethane ,1,1-Trifluoroethane 143a 7. Dichlorodifluoromethane Chlorodifluoromethane ,1,1,2-Tetrafluoroethane 134a 10. Chloromethane ,1,2,2-Tetrafluoroethane Bromochlorodifluoromethane 12B1 Compound Freon # 13. 1,1-Difluoroethane 152a 14. 1,2-Dichloro-1,1,2,2-tetrafluoroethane Chloro-1,1,1,2-tetrafluoroethane Chloro-1,1-difluoroethane 142b 17. Dichlorofluoromethane Trichlorofluoromethane Chloroethane Dichloromethane 21. 1,1-Dichloro-1-fluoroethane 141b 22. 2,2-Dichloro-1,1,1-trifluoroethane ,1,2-Trichloro-1,2,2-trifluoroethane ,2-Dibromo-1,1,2,2-tetrafluoroethane 114B C v priced-ap.p /13/01, 11:43 PM

58 EPA Air Analysis Compendium Method TO-14 Standard Column: DB-1 60 m x 0.32 mm I.D., 1.0 µm P/N: Carrier: Helium at 25 cm/sec measured off of CO 2 at 35 C constant flow mode Oven: 35 C for 5 min C at 5 /min C at 30 /min 220 C for 5 min Injector: Entech 7100 cryogenic sample preconcentrator 400 ml of a 10 ppbv TO-14 standard and 100 ml of a 20 ppbv IS/SS standard Detector: MSD, full scan of m/z Chromatogram courtesy of Dan Cardin at Entech Instruments, Inc. (Simi Valley, CA; 1. CO 2 2. Freon 12 (Dichlorodifluoromethane) 3. Chloromethane 4. Freon 114 (1,2-Dichloro-1,1,2,2-tetrafluoroethane) 5. Vinyl chloride 6. Bromomethane 7. Chloroethane 8. Freon 11 (Trichlorofluoromethane) 9. 1,1-Dichloroethene 10. Methylene chloride 11. Freon 113 (1,1,2-Trichloro-1,2,2-trifluoroethane) 12. 1,1-Dichloroethane 13. cis-1,2-dichloroethene 14. Bromochloromethane (IS) 15. Chloroform 16. 1,2-Dichloroethane 17. 1,1,1-Trichloroethane 18. Benzene 19. Carbon tetrachloride 20. 1,4-Difluorobenzene (IS) 21. 1,2-Dichloropropane 22. Trichloroethene 23. cis-1,3-dichloropropene 24. trans-1,3-dichloropropene 25. 1,1,2-Trichloroethane 26. Toluene-d 8 (SS) 27. Toluene 28. 1,2-Dibromoethane 29. Tetrachloroethene 30. Chlorobenzene-d 5 (SS) 31. Chlorobenzene 32. Ethylbenzene 33. m-xylene 34. p-xylene 35. Styrene 36. 1,1,2,2-Tetrachloroethane 37. o-xylene Bromofluorobenzene (SS) 39. 1,3,5-Trimethylbenzene 40. 1,2,4-Trimethylbenzene 41. 1,3-Dichlorobenzene 42. 1,2-Dichlorobenzene 43. 1,4-Dichlorobenzene 44. 1,2,4-Trichlorobenzene 45. 1,2-Dibromobenzene (IS) 46. Hexachloro-1,3-butadiene Environmental Ion (90.70 to 91.70): D Peak 37 m/z 91 Peak 36 m/z C v priced-ap.p /13/01, 11:44 PM

59 Environmental Chromatograms EPA Volatiles by GC/MS (Split Injector) Column: DB m x 0.25 mm I.D., 1.4 µm P/N: Carrier: Helium at 31 cm/sec, measured at 40 C Oven: 40 C for 3 min C at 8 /min 90 C for 4 min C at 6 /min 200 C for 5 min Injector: Purge and trap (O.I.A. 4560) Sample: 40 ppb per each component in 5 ml water Purge: Helium for 11 min at 40 ml/min Trap: Tenax/Silica Gel/Carbosieve TM Desorb: 0.6 min at 220 C Interface: Split injector at 110 C with split flow set at 30 ml/min Detector: MSD, 235 C transfer line, full scan amu (m/z 44 subtracted) Compound list for both chromatograms (NOTE: Some compounds not present in both chromatograms) 1. Dichlorodifluoromethane 2. Chloromethane 3. Vinyl chloride 4. Bromomethane 5. Chloroethane 6. Trichlorofluoromethane 7. Diethyl ether 8. 1,1-Dichloroethene 33,34 9. Acetone 10. Iodomethane 11. Carbon disulfide 12. Allyl chloride 13. Methylene chloride 14. Acrylonitrile 15. Methyl-tert-butyl ether 16. trans-1,2-dichloroethene 17. Hexane 18. 1,1-Dichloroethane Butanone 20. cis-1,2-dichloroethene 21. 2,2-Dichloropropane 22. Propionitrile 23. Methyl acrylate 24. Methacrylonitrile 48 52,53 15, ,20,21, , , , , Bromochloromethane 26. Tetrahydrofuran 27. Chloroform 28. Pentafluorobenzene (IS) 29. 1,1,1-Trichloroethane 61, , , Chlorobutane 31. 1,1-Dichloropropene 32. Carbon tetrachloride 33. Benzene 34. 1,2-Dichloroethane 35. 2,2-Dimethylhexane 36. Fluorobenzene (IS) 37. 1,4-Difluorobenzene (IS) 38. Trichloroethene 39. 1,2-Dichloropropane 40. Methyl methacrylate (compound list continued below) Column: DB-VRX 60 m x 0.25 mm I.D., 1.4 µm P/N: Carrier: Helium at 30 cm/sec, measured at 45 C Oven: 45 C for 10 min C at 12 /min 190 C for 2 min C at 6 /min 225 C for 1 min Injector: Purge and trap (O.I.A. 4560) Sample: 40 ppb per each component in 5 ml water Purge: Helium for 11 min at 40 ml/min Trap: Tenax, Silica gel,carbosieve Desorb: 0.6 min at 220 C Interface: Split injector at 110 C with split flow set at 30 ml/min Detector: MSD, 235 C transfer line full scan amu (m/z 44 subtracted) Compound list (continued) 41. Dibromomethane 42. Bromodichloromethane Nitropropane 44. Chloroacetonitrile 45. cis-1,3-dichloropropene Methyl-2-pentanone 47. 1,1-Dichloro-2-propanone 48. Toluene 49. trans-1,3-dichloropropene 50. Ethyl methacrylate 51. 1,1,2-Trichloroethane 52. Tetrachloroethene , ,3-Dichloropropane Hexanone 55. Dibromochloromethane 56. 1,2-Dibromoethane Chloro-3-fluorobenzene (IS) 58. Chlorobenzene 59. 1,1,1,2-Tetrachloroethane 60. Ethylbenzene 61. m-xylene 62. p-xylene 63. o-xylene 64. Styrene 65. Bromoform 66. Isopropylbenzene Bromofluorobenzene (SS) 68. 1,1,2,2-Tetrachloroethane 69. Bromobenzene 70. 1,2,3-Trichloropropane 71. trans-1,4-dichloro-2-butene 72. n-propylbenzene Chlorotoluene 74. 1,3,5-Trimethylbenzene 33 29, ,42 47, , Chlorotoluene 76. tert-butylbenzene 77. Pentachloroethane 78. 1,2,4-Trimethylbenzene 79. sec-butylbenzene ,62 68, ,3-Dichlorobenzene 81. p-isopropyltoluene 82. 1,4-Dichlorobenzene 83. n-butylbenzene 84. 1,2-Dichlorobenzene 85. Hexachloroethane 86. 1,2-Dibromo-3-chloropropane 87. Nitrobenzene 88. 1,2,4-Trichlorobenzene 89. Hexachlorobutadiene 90. Naphthalene 91. 1,2,3-Trichlorobenzene SS - Surrogate Standard IS - Internal Standard C378a&b 58 v priced-ap.p /14/01, 1:27 AM

60 Chromatograms EPA Method 8330 Column: DB-1 12 m x 0.53 mm I.D., 1.0 µm P/N: J* Carrier: Hydrogen at 12 ml/min, measured at 80 C Oven: C at 20 /min Injector: Cool On-column 3.0 µl of a standard extracted from water in isoamyl acetate, 2.5 µg/l each component Detector: ECD, 250 C Nitrogen makeup gas at 35 ml/min Environmental 1. Nitrobenzene 2. 2-Nitrotoluene 3. 3-Nitrotoluene 4. 4-Nitrotoluene 5. Nitroglycerin 6. 1,3-Dinitrobenzene 7. 2,6-Dinitrotoluene 8. 2,4-Dinitrotoluene 9. 3,4-Dinitrotoluene (SS) 10. 1,3,5-Trinitrobenzene 11. 2,4,6-Trinitrotoluene 12. Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) Amino-2,6-dinitrotoluene Amino-4,6-dinitrotoluene 15. Methyl-2,4,6-trinitrophenylnitramine (Tetryl) 16. Octahydro-1,3,5,7-tetranitro- 1,3,5,7-tetrazocine (HMX) SS - Surrogate Standard *Note: Part number J, a 15 meter column, was cut down to 12 meters for the application 0 11 C658 Phenoxy Acid Methyl Esters Column: P/N: Carrier: Helium at 32 cm/sec, measured at 50 C Oven: 50 C for 1 min C at 25 /min C at 10 /min 320 C for 1 min Injector: Splitless, 250 C 30 sec purge activation time 2 µl x ng/µl solution in acetone Detector: MSD, full scan transfer line 300 C 1. Dicamba 2. MCPP 3. MCPA 4. Dichloroprop 5. 2,4-D 6. Pentachlorophenol 7. 2,4,5-T ,4,5-TP 9. 2,4-DB 10. Dinoseb 11. Picloram DB-XLB 30 m x 0.25 mm I.D., 0.25 µm Haloacetic Acid Methyl Esters (Optimized for Resolution) Column: DB m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 24 cm/sec, measured at 35 C Oven: 35 C for 10 min, C at 5 /min, 75 C for 15 min, C at 5 /min, 100 C for 5 min, C at 5 /min, 135 C for 5 min Injector: Splitless, 200 C, 30 sec purge activation time Detector: ECD, 260 C Nitrogen makeup gas at 30 ml/min 1. Chloroacetic acid 2. Bromoacetic acid 3. Dichloroacetic acid 4. Dalapon 5. Trichloroacetic acid 6. Bromochloroacetic acid 7. 1,2,3-Trichloropropane (ISTD)* 8. Dibromoacetic acid 9. Bromodichloroacetic acid 10. Chlorodibromoacetic acid 11. 2,3-Dibromopropionic acid (SSTD) 12. Tribromoacetic acid 6 *nonmethylated C412 C v priced-ap.p /27/01, 10:21 AM

61 Environmental Chromatograms Volatile Organics by Purge and Trap Column: DB m x 0.53 mm I.D., 3.0 µm P/N: A4 Carrier: Helium at 10 ml/min, measured at 35 C Oven: 35 C for 10 min C at 4 /min 200 C for 5 min Injector: Purge and trap (O.I.A. 4560) 40 ppb per component in 5 ml water Trap: Tenax/Silica Gel/Charcoal Detector: PID (O.I.A. 4430), 200 C 35,36 46,45 For compound list see page C575 Column: DB m x 0.45 mm I.D., 2.55 µm P/N: Carrier: Helium at 9 ml/min, measured at 35 C Oven: 35 C for 6 min C at 8 /min 200 C for 3.5 min Injector: Purge and trap (O.I.A. 4560) 40 ppb per component in 5 ml water Trap: Tenax/Silica Gel/Charcoal Detector: PID (O.I.A. 4430), 200 C High Speed Megabore Saves 23 Minutes! 35,36 46, C v priced-ap.p /24/01, 2:31 PM

62 Chromatograms Purgeables Analysis (EPA Method 502.2) Column: DB m x 0.53 mm I.D., 3.0 µm P/N: A4 Carrier: Helium at 10 ml/min, measured at 35 C Oven: 35 C for 10 min C at 4 /min 200 C for 5 min Injector: Purge and trap (O.I.A. 4560) 40 ppb per component in 5 ml water Trap: Tenax/Silica Gel/Charcoal Detector: ELCD (O.I.A. 4420), with NiCat reaction tube in the halogen mode, 950 C reactor temperature Compound list for both chromatograms 1. Dichlorodifluoromethane 2. Chloromethane 3. Vinyl chloride 4. Bromomethane 5. Chloroethane 6. Trichlorofluoromethane 7. 1,1-Dichloroethene 8. Methylene chloride 9. trans-1,2-dichloroethene 10. 1,1-Dichloroethane 11. cis-1,2-dichloroethene 12. 2,2-Dichloropropane 13. Bromochloromethane Chloroform 15. 1,1,1-Trichloroethane ,1-Dichloropropene Carbon tetrachloride 18. Benzene 19. 1,2-Dichloroethane 20. Trichloroethene 21. 1,2-Dichloropropane 22. Dibromomethane 23. Bromodichloromethane 24. cis-1,3-dichloropropene 25. Toluene 26. trans-1,3-dichloropropene 27. 1,1,2-Trichloroethane 28. Tetrachloroethene 29. 1,3-Dichloropropane 30. Dibromochloromethane 31. 1,2-Dibromoethane 32. Chlorobenzene 33. 1,1,1,2-Tetrachloroethane 34. Ethylbenzene 35. m-xylene 36. p-xylene 37. o-xylene 38. Styrene Bromoform 40. Isopropylbenzene 41. 1,1,2,2-Tetrachloroethane 42. Bromobenzene 43. 1,2,3-Trichloropropane 44. n-propylbenzene Chlorotoluene 46. 1,3,5-Trimethylbenzene Chlorotoluene 48. tert-butylbenzene 49. 1,2,4-Trimethylbenzene 50. sec-butylbenzene 51. 1,3-Dichlorobenzene 52. p-isopropyltoluene 53. 1,4-Dichlorobenzene 54. n-butylbenzene 55. 1,2-Dichlorobenzene 56. 1,2-Dibromo-3-chloropropane 57. 1,2,4-Trichlorobenzene 58. Hexachlorobutadiene 59. Naphthalene 60. 1,2,3-Trichlorobenzene Environmental C384 Column: DB m x 0.45 mm I.D., 2.55 µm P/N: Carrier: Helium at 9 ml/min, measured at 35 C Oven: 35 C for 6 min C at 8 /min 200 C for 3.5 min Injector: Purge and trap (O.I.A. 4560) 40 ppb per component in 5 ml water Trap: Tenax/Silica Gel/Charcoal Detector: ELCD (O.I.A. 4420) with NiCat reaction tube in the halogen mode, 950 C reactor temperature High Speed Megabore Saves 23 Minutes! C v priced-ap.p /24/01, 2:31 PM

63 Environmental Chromatograms Extended Analyte List for EPA Method 8021 DB-624/VRX Dual Column Columns: DB m x 0.45 mm I.D., 2.55 µm DB-VRX 75 m x 0.45 mm I.D., 2.55 µm Guard Column: 5 m x 0.53 mm I.D. deactivated fused silica 3-way universal glass union P/N: Carrier: Helium at 9 ml/min, measured at 35 C Oven: 35 C for 12 min C at 5 /min 60 C for 1 min C at 17 /min 200 C for 5 min Injector: Purge and trap (O.I.A. 4560) J&W LVI (Low Volume Injector), 150 C 20 ppb per component in 5 ml water Trap: VOCARB TM 3000 Preheat: 175 C Desorb: 260 C for 1 min Detector A: PID (O.I.A. 4430), 200 C Helium makeup gas at 20 ml/min Detector B: ELCD (O.I.A. 4420), with NiCat TM reaction tube in the halogen mode, 950 C reactor temperature Compound list for all chromatograms 1. Dichlorodifluoromethane 2. Chloromethane 3. Vinyl chloride 4. Bromomethane 5. Chloroethane 6. Trichlorofluoromethane 7. 2-Chloropropane (IS) 8. 1,1-Dichloroethene 9. Iodomethane 10. Allyl chloride 11. Methylene chloride 12. trans-1,2-dichloroethene 13. 1,1-Dichloroethane 14. Chloroprene 15. cis-1,2-dichloroethene 16. 2,2-Dichloropropane 17. Bromochloromethane 18. Chloroform 19. 1,1,1-Trichloroethane 20. Carbon tetrachloride 21. 1,1-Dichloropropene 22. Benzene 23. 1,2-Dichloroethane 24. Fluorobenzene (IS) 25. Trichloroethene 26. 1,2-Dichloropropane 27. Dibromomethane 28. Trifluorotoluene (IS) 29. Bromodichloromethane Chloroethyl vinyl ether 31. cis-1,3-dichloropropene 32. Toluene 33. trans-1,3-dichloropropene 34. 1,1,2-Trichloroethane 35. Tetrachloroethene 36. 1,3-Dichloropropane 37. Dibromochloromethane 38. 1,2-Dibromoethane Chloro-3-fluorobenzene (IS) 40. Chlorobenzene 41. 1,1,1,2-Tetrachloroethane 42. Ethylbenzene 43. m-xlyene 44. p-xylene 45. Styrene 46. o-xylene 47. Bromoform 48. Isopropylbenzene 49. cis-1,4-dichlorobutene 50. 1,1,2,2-Tetrachloroethane 51. Bromobenzene 52. 1,2,3-Trichloropropane 53. n-propylbenzene Chlorotoluene 55. 1,3,5-Trimethylbenzene Chlorotoluene 57. tert-butylbenzene 58. 1,2,4-Trimethylbenzene 59. sec-butylbenzene 60. 1,3-Dichlorobenzene 61. p-isopropyltoluene 62. 1,4-Dichlorobenzene 63. Benzyl chloride 64. n-butylbenzene 65. 1,2-Dichlorobenzene 66. bis (2-Chloroisopropyl) ether 67. 1,2-Dibromo-3-chloropropane 68. 1,2,4-Trichlorobenzene 69. Hexachlorobutadiene 70. Naphthalene 71. 1,2,3-Trichlorobenzene 35,36 Detector B: ELCD DB , ,21 15, DB-VRX , , , C v priced-ap.p /24/01, 2:31 PM

64 Chromatograms Detector A: PID DB ,61 60, , , , see inset Environmental 43, see inset DB-VRX , made by elves with really big chromatograms 63 v priced-ap.p /24/01, 2:31 PM

65 Environmental Chromatograms CLP Pesticides Column: DB-17ms 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 45 cm/sec, measured at 110 C Oven: 110 C for 0.5 min C at 25 /min C at 12 /min C at 15 /min 320 C for 2 min Injector: Splitless, 250 C 30 sec purge activation time 50 pg composite 8081 standard, and CLP-034 surrogate mix, 1 Accustandard Inc. Detector: ECD, 350 C Nitrogen makeup gas at 40 ml/min 1. Tetrachloro-m-xylene (SS) 2. α-bhc 3. γ-bhc 4. β-bhc 5. Heptachlor 6. δ-bhc 7. Aldrin 8. Heptachlor epoxide 9. γ-chlordane 10. α-chlordane 11. Endosulfan I 12. p,p -DDE Dieldrin 14. Endrin 15. p,p -DDD 16. Endosulfan II 17. p,p -DDT 18. Endrin aldehyde 19. Endosulfan sulfate 20. Dibutylchlorendate (SS) 21. Methoxychlor 22. Endrin ketone SS - Surrogate Standard Standards used were a composite of individual solutions supplied courtesy of Accustandard Inc C CLP Pesticides Column: DB-XLB 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 45 cm/sec, measured at 110 C Oven: 110 C for 0.5 min C at 25 /min C at 12 /min C at 15 /min 320 C for 2 min Injector: Splitless, 250 C 30 sec purge activation time 50 pg composite 8081 standard, and CLP-034 surrogate mix, Accustandard Inc. Detector: ECD, 350 C Nitrogen makeup gas at 40 ml/min Tetrachloro-m-xylene (SS) 2. α-bhc 3. γ-bhc 4. β-bhc 5. Heptachlor 6. δ-bhc 7. Aldrin 8. Heptachlor epoxide 9. γ-chlordane 10. α-chlordane 11. Endosulfan I 12. p,p -DDE Dieldrin 14. Endrin 15. p,p -DDD 16. Endosulfan II 17. p,p -DDT 18. Endrin aldehyde 19. Endosulfan sulfate 20. Dibutylchlorendate (SS) 21. Methoxychlor 22. Endrin ketone SS - Surrogate Standard Standards used were a composite of individual solutions supplied courtesy of Accustandard Inc C v priced-ap.p /24/01, 2:32 PM

66 Chromatograms Organochlorine Pesticides Column: P/N: DB-35ms 30 m x 0.25 mm I.D., 0.25 µm Carrier: Helium at 45 cm/sec, measured at 110 C Oven: 110 C for 0.5 min C at 25 /min C at 12 /min C at 15 /min 320 C for 2 min Injector: Splitless, 250 C 30 sec purge activation time 50 pg composite 8081 standard, and CLP-034 surrogate mix, Accustandard Inc. Detector: ECD, 350 C Nitrogen makeup gas at 40 ml/min Tetrachloro-m-xylene (SS) 2. α-bhc 3. γ-bhc 4. β-bhc 5. Heptachlor 6. δ-bhc 7. Aldrin 8. Heptachlor epoxide 9. γ-chlordane 10. α-chlordane 11. Endosulfan I 12. 4,4 -DDE 13. Dieldrin 14. Endrin 15. 4,4 -DDD 16. Endosulfan II 17. 4,4 -DDT 18. Endrin aldehyde 19. Endosulfan sulfate 20. Dibutylchlorendate (SS) 21. Methoxychlor 22. Endrin ketone SS - Surrogate Standard Environmental 21 C CLP Pesticides Column: DB-5 15 m x 0.32 mm I.D., 0.25 µm 10 m DuraGuard Column P/N: Carrier: Helium at 37.2 cm/sec, measured at 50 C Oven: 50 C for 1 min C at 25 /min C at 4 /min Injector: Splitless, 250 C 30 sec purge activation time Detector: FID, 300 C α-bhc 2. β-bhc 3. γ-bhc 4. δ-bhc 5. Heptachlor 6. Aldrin 7. Heptachlor epoxide 8. γ-chlordane 9. Endosulfan I 10. α-chlordane 11. Dieldrin 12. p,p -DDE 13. Endrin 14. Endosulfan II 15. p,p -DDD 16. Endrin aldehyde 17. Endosulfan sulfate 18. p,p -DDT 19. Endrin ketone 20. Methoxychlor C v priced-ap.p /24/01, 2:32 PM

67 Environmental Chromatograms Organochlorine Pesticides (EPA Method 8081A) Column: 1 DB-5ms 30 m x 0.25 mm I.D., 0.25 P/N: Carrier: Helium at 35 cm/sec, measured at 50 C Oven: 50 C for 1 min C at 25/min C at 5/min 300 C for 5 min Injector: Splitless, 250 C 30 sec purge activation time 1 µl of 35 µg/ml composite 8081A standard, Accustandard Inc. Detector: MSD, full scan at m/z C transfer line ,2-Dibromo-3-chloropropane 2. 4-Chloro-3-nitrobenzotrifluoride (SS) 3. Hexachloropentadiene 4. 1-Bromo-2-nitrobenzene (IS) 5. Terrazole 6. Chloroneb 7. Trifluralin 8. 2-Bromobiphenyl (SS) 9. Tetrachloro-m-xylene (SS) 10. α, α-dibromo-m-xylene 11. Propachlor 12. Di-allate A 13. Di-allate B 14. Hexachlorobenzene 15. α-bhc 16. Pentachloronitrobenzene (IS) 17. γ-bhc 18. β-bhc 19. Heptachlor Alachlor 21. δ-bhc 22. Chlorothalonil 23. Aldrin 24. Dacthal 25. Isodrin 26. Kelthane 27. Heptachlor epoxide 28. γ-chlordane 29. trans-nonachlor 30. α-chlordane 31. Endosulfan I 32. Captan 33. p, p -DDE , , , Dieldrin 35. Chlorobenzilate 36. Perthane 37. Chloropropylate 38. Endrin 39. p, p -DDD 40. Endosulfan II 41. p, p -DDT 42. Endrin aldehyde 43. Endosulfan sulfate 44. Dibutylchlorendate (SS) 45. Captafol 46. Methoxychlor 47. Endrin ketone 48. Mirrex TM 49. cis-permethrin 50. trans-permethrin * Breakdown Products SS - Surrogate Standard IS - Internal Standard * C Organochlorine Pesticides (EPA Method 8081A) Column: 1 DB-35ms 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 35 cm/sec, measured at 50 C Oven: 50 C for 1 min C at 25 /min C at 5 /min 300 C for 5 min Injector: Splitless, 250 C 30 sec purge activation time 1 µl of 35 µg/ml composite 8081A standard, Accustandard Inc. Detector: MSD, 300 C transfer line full scan at m/z ,2-Dibromo-3-chloropropane 2. 4-Chloro-3-nitrobenzotrifluoride (SS) 3. Hexachloropentadiene 4. 1-Bromo-2-nitrobenzene (IS) 5. Terrazole 6. Chloroneb 3 7. Trifluralin 8. 2-Bromobiphenyl (SS) Tetrachloro-m-xylene (SS) 10. α,α-dibromo-m-xylene 11. Propachlor 12. Di-allate A 13. Di-allate B 14. Hexachlorobenzene 15. α-bhc 16. Pentachloronitrobenzene (IS) 17. γ-bhc 18. β-bhc 6 8, Heptachlor 20. Alachlor 21. δ-bhc 22. Chlorothalonil 23. Aldrin 24. Dacthal TM 25. Isodrin 26. Kelthane 27. Heptachlor epoxide 28. γ-chlordane 29. trans-nonachlor 30. α-chlordane 31. Endosulfan I 32. Captan 33. p,p -DDE 34. Dieldrin 35. Chlorobenzilate TM 32, Perthane TM 37. Chloropropylate 38. Endrin 39. p,p -DDD Endosulfan II 41. p,p -DDT 42. Endrin aldehyde 43. Endosulfan sulfate 44. Dibutylchlorendate (SS) 45. Captafol 46. Methoxychlor 47. Endrin ketone 48. Mirrex 49. cis-permethrin 50. trans-permethrin * Breakdown Products SS - Surrogate Standard IS - Internal Standard Standards used were a composite of individual solutions supplied courtesy of Accustandard Inc * C v priced-ap.p /24/01, 2:32 PM

68 Chromatograms Organophosphorous Pesticides (EPA Method 8141 A) Column: Carrier: DB-5ms 30 m x 0.25 mm I.D., 0.25 µm P/N: Oven: 50 C for 1 min C at 25 /min C at 5 /min 300 C for 5 min Helium at 35 cm/sec, measured at 50 C Injector: Splitless, 250 C 30 sec purge activation time 1 µl of 40 µg/ml 8141A standard, Accustandard Inc. Detector: MSD, full scan 300 C transfer line , Chloro-3- nitrotrifluoride 2. Dichlorvos 3. Hexamethylphosphoramide 4. Mevinphos 5. TEPP 6. Thionazin 7. Demeton-O 8. Ethoprop 9. Naled 10. Dicrotophos 11. Sulfotepp 12. Monocrotophos 13. Phorate Dimethoate 31. Chlorpyrifos 15. Demeton-S 32. Fenthion 16. Simazine 33. Ethyl parathion 17. Atrazine 34. Trichloronate 18. Dioxathion 35. Chlorfenvinphos 19. Terbufos 36. Crotoxyphos 20. Fonofos 37. Stirophos 21. Diazinon 38. Tokuthion 22. Disulfoton 39. Merphos 23. Phosphamidon 40. Fensulfothion 24. Dichlofenthion 41. Ethion 25. Chlorpyrifos methyl 42. Bolstar 26. Methyl parathion 43. Famphur 27. Ronnel 44. Carbophenothion 28. Fenitrothion 45. Phosmet 29. Malathion 46. EPN 30. Aspon 47. Leptophos 48. Azinphos methyl 49. Tri-o-cresyl phosphate 50. Azinphos ethyl 51. Coumaphos 49 (Peak not shown on DB-1701) * Breakdown products Environmental 5 * * * * C Organophosphorous Pesticides (EPA Method 8141A) Column: DB-35ms 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 35 cm/sec, measured at 50 C Oven: 50 C for 1 min C at 25 /min C at 5 /min 300 C for 5 min Injector: Splitless, 250 C 30 sec purge activation time 1 µl of 40 µg/ml 8141A standards, Accustandard Inc. Detector: MSD, full scan at m/z C transfer line Note: All standards used were supplied courtesy of Accustandard Inc. 1 * 2 3 * 4 * 1. 4-Chloro-3-nitrotrifluoride 2. Dichlorvos 3. Hexamethylphosphoramide 4. Mevinphos 5. TEPP 6. Thionazin 7. Demeton-O 8. Ethoprop 9. Naled 10. Dicrotophos 11. Sulfotepp 12. Monocrotophos 13. Phorate 14. Dimethoate 15. Demeton-S 16. Simazine 17. Atrazine 18. Dioxathion 19. Terbufos 20. Fonofos , * , Diazinon 22. Disulfoton 23. Phosphamidon 24. Dichlofenthion 25. Chlorpyrifos methyl 26. Methyl parathion 27. Ronnel 33 * 29, 31, , Fenitrothion 29. Malathion 30. Aspon 31. Chlorpyrifos 32. Fenthion 33. Ethyl parathion 34. Trichloronate 35. Chlorfenvinphos 36. Crotoxyphos 37. Stirophos 38. Tokuthion 39. Merphos 40. Fensulfothion 41. Ethion 42. Bolstar 43. Famphur 44. Carbophenothion 45. Phosmet 46. EPN 47. Leptophos 48. Azinphos methyl 49. Tri-o-cresyl phosphate 50. Azinphos ethyl 51. Coumaphos * Breakdown products C v priced-ap.p /24/01, 2:32 PM

69 Environmental Chromatograms Environmental Analyte Inertness Mix Column: DB-1ms 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Hydrogen at 39.8 cm/sec, measured at 125 C Oven: 125 C for 8.5 min C at 20 /min Injector: Split 1:50, 250 C Detector: FID, 300 C 1. 2-Ethylhexanoic acid* 2. 1,6-Hexanediol* 3. 4-Chlorophenol* 4. 1-Methylnaphthalene 5. Tridecane 6. 1-Undecanol 7. Tetradecane 8. Dicyclohexylamine* 9. 2,4-Dinitrophenol* 10. Acenaphthene 11. N-Nitrosodiphenylamine* 12. Pentachlorophenol* 13. Phenanthrene 14. Carbazole *Active analytes * * 7 2* 3* 6 8* 9* 12* C EPA Method 8061 (Phthalate Esters) Column: DB-5ms 20 m x 0.18 mm I.D., 0.18 µm P/N: Carrier: Helium at 49 cm/sec, measured at 80 C constant flow program Oven: 80 C for 0.5 min C at 30 /min C at 15 /min Injector: Splitless, 300 C 30 sec purge activation time 1 µl of 20 ng/µl Method 8061 mixture (Accustandard) in hexane Detector: MSD, m/z full scan 325 C transfer line Dimethyl phthalate 2. Diethyl phthalate 3. Benzyl benzoate (IS) 4. Diisobutyl phthalate 5. Di-n-butyl phthalate 6. bis(4-methoxyethyl) phthalate 7. bis(4-methyl-2-pentyl) phthalate *Two isomers 8. bis(2-ethoxyethyl) phthalate 9. Diamyl phthalate 10. Dihexyl phthalate 11. Butyl benzyl phthalate Hexyl 2-ethylhexyl phthalate 13. bis(2-n-butoxyethyl) phthalate 14. Dicyclohexyl phthalate 15. bis(2-ethylhexyl) phthalate 16. Diphenyl phthalate (SS 17. Diphenyl isophthalate (SS) 18. Di-n-octyl phthalate 19. Dibenzyl phthalate (SS) 20. Dinonyl phthalate * Two isomers SS - Surrogate Standard * C v priced-ap.p /24/01, 2:33 PM

70 Chromatograms EPA Method Column: DB-5ms 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium, at 32 cm/sec, measured at 45 C, constant flow mode Oven: 45 C for 1 min C at 30 /min 130 C for 3 min C at 12 /min C at 7 /min C at 12 /min 325 C for 5 min Injector: Splitless 1.0 min purge activation time, 300 C FocusLiner Detector: MSD, 325 C transfer line full scan at m/z Sample: Composite mixture of Accustandard Method standards (M SV-ASL, M FS-ASL, M CP-ASL, M NP1-ASL, M NP2-ASL): target compounds at 2 ng/µl, IS/SS at 5 ng/µl Peak No./CompoundRT m/z 1. Isophorone ,3-Dimethyl-2- nitrobenzene (SS) Dichlorvos Hexachlorocyclopentadiene EPTC Mevinphos Butylate / Vernolate Dimethyl phthalate Terrazole (aka Etridazole) / ,6-Dinitrotoluene Tillam (aka Pebulate) Acenaphthylene Acenaphthene-d10 (IS #1) Chloroneb Chlorobiphenyl Tebuthiuron ,4-Dinitrotoluene Molinate Diethyl phthalate Fluorene Propachlor Ethoprop Cycloate / Chlorpropham Trifluralin α-bhc Peak No./CompoundRT m/z 28. 2,3-Dichlorobiphenyl / Hexachlorobenzene Gesatamine (aka Atraton) / Prometon / Atrazine / Simazine / β-bhc Pentachlorophenol Propazine / γ-bhc Terbufos Pronamide Diazinon / Phenanthrene-d10 (IS #2) Chlorothalonil Phenanthrene Terbacil Methyl paraoxon Disulfoton Anthracene δ-bhc ,4,5-Trichlorobiphenyl Metribuzin Alachlor Simetryn Ametryn / Heptachlor Prometryne / Prebane (aka Terbutryne) / Bromacil Di-n-butyl phthalate ,2',4,4'-Tetrachlorobiphenyl Metolachlor Dursban (aka Chlorpyrifos) / Cyanazine / Dacthal (aka DCPA methyl ester) Aldrin Triadimefon Diphenamid / MGK-264 (isomer A) / MGK-264 (isomer B) Heptachlor epoxide ,2',3',4,6-Pentachlorobiphenyl Merphos / γ-chlordane Tetrachlorvinphos (aka Stirifos) Peak No./CompoundRT m/z 74. Butachlor / Pyrene-d10 (SS) Pyrene α-chlordane / Endosulfan I trans-nonachlor Fenamiphos / Napropamide Tricyclazole p,p -DDE DEF / ,2',4,4',5,6'-Hexachlorobiphenyl Dieldrin Carboxin Endrin / Chlorobenzilate Endosulfan II p,p -DDD / Endrin aldehyde Norflurazon Benzyl butyl phthalate Endosulfan sulfate p,p -DDT / Hexazinone bis (2-Ethylhexyl) adipate Triphenylphosphate (SS) / Endrin ketone (breakdown product) / ,2',3,3',4,4',6-Heptachlorobiphenyl / Benz[a]anthracene Chrysene-d12 (IS #3) ,2',3,3',4,5',6,6'-Octachlorobiphenyl / Methoxychlor Chrysene bis(2-ethylhexyl) phthalate Fenarimol cis-permethrin trans-permethrin Benzo[b]fluoranthene Benzo[k]fluoranthene Fluridone Benzo[a]pyrene Perylene-d12 (SS) Indeno[1,2,3-c,d]pyrene Dibenz[a,h]anthracene Benzo[g,h,i]perylene SS - Surrogate Standard IS - Internal Standard Environmental C v priced-ap.p /24/01, 2:33 PM

71 Environmental Chromatograms EPA Method 8270 Column: DB-5ms 30 m x 0.25 mm I.D., 0.5 µm P/N: Carrier: Helium at 34.4 cm/sec, measured at 150 C Oven: 40 C for 5 min C at 12 /min 290 C for 6 min C at 20 /min 325 C for 5 min Injector: Splitless, 250 C 45 sec purge activation time 1 µl of 160 ng/µl, AccuStandard Inc. EPA Method 8270 standard Detector: MSD, 325 C transfer line ,30,31 Compound list for all chromatograms at right (page 71) 16,17 25, , , ,58,59 50, ,62 67,68,69 C , ,75 76 C , , ,91 92 C v priced-ap.p /24/01, 2:33 PM

72 Chromatograms EPA Method 8270 Compound List Compound m/z RT 1. N-Nitrosodimethylamine Picoline Methyl methanesulfonate Ethyl methanesulfonate Phenol Aniline bis(2-chloroethyl) ether Chlorophenol ,3-Dichlorobenzene ,4-Dichlorobenzene Benzyl alcohol ,2-Dichlorobenzene Methyl phenol bis(2-chloroisopropyl) ether Methyl phenol Acetophenone N-Nitrosodi-n-propylamine Hexachloroethane Nitrobenzene N-Nitrosopiperidine Isophorone Nitrophenol ,4-Dimethylphenol bis(2-chloroethoxy)methane Benzoic acid ,4-Dichlorophenol ,2,4-Trichlorobenzene Naphthalene (α, α)-dimethylphenethylamine Chloroaniline ,6-Dichlorophenol Hexachlorobutadiene N-Nitroso-di-n-butylamine Chloro-3-methylphenol Methylnaphthalene Hexachlorocyclopentadiene ,2,4,5-Tetrachlorobenzene Compound m/z RT 38. 2,4,6-Trichlorophenol ,4,5-Trichlorophenol Chloronaphthalene Chloronaphthalene Nitroaniline Dimethyl phthalate ,6-Dinitrotoluene Acenaphthylene Nitroaniline Acenaphthene ,4-Dinitrophenol Nitrophenol Pentachlorobenzene ,4-Dinitrotoluene Dibenzofuran Naphthylamine ,3,4,6-Tetrachlorophenol Naphthylamine Diethyl phthalate Chlorophenyl phenyl ether Fluorene Nitroaniline ,6-Dinitro-2-methylphenol Diphenylamine Decomposed N-Nitrosodiphenylamine ,2-Diphenylhydrazine Phenacetin Bromophenyl phenyl ether Hexachlorobenzene Aminobiphenyl Pentachlorophenol Pentachloronitrobenzene Pronamide Phenanthrene Anthracene Di-n-butyl phthalate Compound m/z RT 74. Fluoranthene Benzidine Pyrene p-dimethylaminoazobenzene Butyl benzyl phthalate ,3'-Dichlorobenzidine bis(2-ethylhexyl)phthalate Benzo[a]anthracene Chrysene Di-n-octyl phthalate ,12-Dimethylbenz[a]anthracene Benzo[b]fluoranthene Benzo[k]fluoranthene Benzo[a]pyrene Methylcholanthrene Dibenz[a,j]acridine Indeno[1,2,3-c,d]pyrene Dibenz[a,h]anthracene Benzo[g,h,i]perylene Environmental EPA Method 8082 PCB Congeners Column: DB-1ms 30 m x 0.25 mm I.D., 0.25 P/N: Carrier: Helium at 46 cm/sec, measured at 100 C, constant pressure mode Oven: 100 C for 0.8 min 100 to 325 C at 15 /min 325 C for 2 min Injector: Splitless, 275 C 0.8 min purge activation time Sample: 1.0 µl injection of a 20 ppm mixture Detector: MSD, 280 C transfer line full scan at m/z PCB congeners are listed by their IUPAC number 1. IUPAC 1 2. Tetrachloro-m-xylene (IS/SS) 3. IUPAC 5 4. IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC Decachlorobiphenyl (IS/SS) IS/SS - Internal Standard/Surrogate Standard C v priced-ap.p /24/01, 2:33 PM

73 Environmental Chromatograms Aroclors (without 1221) Column: DB-XLB 30 m x 0.18 mm I.D., 0.18 µm P/N: Carrier: Helium at 37 cm/sec, measured at 150 C Oven: 100 C for 1 min C at 1.2 /min Injector: Hot on-column, 250 C 1 µl in isooctane, 12.5 ppm Detector: MSD, SIM, 340 C transfer line TIme (min) IUPAC # Retention Time IUPAC # Retention Time IUPAC # Retention Time IUPAC # Retention Time IUPAC # Retention Time C v priced-ap.p /24/01, 2:34 PM

74 Chromatograms Fatty Acid Methyl Esters (FAME) Column: DB m x 0.32 mm I.D., 0.25 µm P/N: Carrier: Helium at 40 cm/sec, measured at 50 C Oven: 50 C for 2 min C at 10 /min 180 C for 5 min C at 5 /min Injector: Splitless, 250 C 45 sec purge activation time Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min 1. C8:0 Methyl octanoate 2. C9:0 Methyl nonanoate 3. C10:0 Methyl decanoate 4. C11:0 Methyl undecanoate 5. C12:0 Methyl laurate 6. C13:0 Methyl tridecanoate 7. C14:0 Methyl myristate 8. C14:1 Methyl myristoleate 9. C15:0 Methyl pentadecanoate 10. C15:1 Methyl 10-pentadecenoate 11. C16:0 Methyl palmitate 12. C16:1 Methyl palmitoleate 13. C17:0 Methyl heptadecanoate 14. C17:1 Methyl 10-heptadecenoate 15. C18:0 Methyl stearate 16. C18:1 trans-methyl elaidate 17. C18:1 cis-methyl oleate 18. C18:2 Methyl linoleate 19. C18:3 Methyl gamma linolenate 20. C18:3 Methyl linolenate 21. C20:0 Methyl arachinoate 22. C20:1 Methyl 11-eicosanoate 23. C20:2 Methyl 11,14-eicosadienoate 24. C20:3 Methyl methyl homogamma linolenate C20:4 Methyl arachidonate 26. C20:3 Methyl 11,14,17-eicosatrienoate 27. C22:0 Methyl behenate 28. C20:5n3 all cis-methyl 5,8,11,14,17-eicosapentenoate (EPA) 29. C22:1 Methyl erucate 30. C22:2 Methyl 13,16-docosadienoate 31. C24:0 Methyl lignocerate 32. C22:6n3 all cis-methyl 4,7,10,13,16,19-docosahexenoate (DHA) 33. C24:1 Methyl nervonate 31 min Food, Flavors, Life Science C070 Fatty Acid Methyl Esters (FAME) Column: DB-WAX 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 40 cm/sec, measured at 50 C Oven: 50 C for 2 min C at 10 /min 250 C for 8 min Injector: Splitless, 250 C 45 sec purge activation time Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min 25. C20:3 Methyl methyl homogamma linolenate 26. C20:4 Methyl arachidonate 27. C20:3 Methyl 11,14,17-eicosatrienoate 28. C22:0 Methyl behenate 29. C20:5n3 all cis-methyl 5,8,11,14,17-eicosapentenoate (EPA) 30. C22:1 Methyl erucate 31. C22:2 Methyl 13,16-docosadienoate 32. C24:0 Methyl lignocerate 33. C22:6n3 all cis-methyl 4,7,10,13,16,19-docosahexenoate (DHA) 34. C24:1 Methyl nervonate 1. C6:0 Methyl hexanoate 2. C8:0 Methyl octanoate 3. C9:0 Methyl nonanoate 4. C10:0 Methyl decanoate 5. C11:0 Methyl undecanoate 6. C12:0 Methyl laurate 7. C13:0 Methyl tridecanoate 8. C14:0 Methyl myristate 9. C14:1 Methyl myristoleate 10. C15:0 Methyl pentadecanoate 11. C15:1 Methyl 10-pentadecenoate 12. C16:0 Methyl palmitate 13. C16:1 Methyl palmitoleate 14. C17:0 Methyl heptadecanoate 15. C17:1 Methyl 10-heptadecenoate 16. C18:0 Methyl stearate 17. C18:1 trans-methyl elaidate 18. C18:1 cis-methyl oleate 19. C18:2 Methyl linoleate 20. C18:3 Methyl gamma linolenate 21. C18:3 Methyl linolenate 22. C20:0 Methyl arachidate 23. C20:1 Methyl 11-eicosanoate 24. C20:2 Methyl 11,14-eicosadienoate , , ,34 made by Ester Elf and her sister Ethyl Elf C min www. 73 v unpriced-ap.p /24/01, 9:48 PM

75 Food, Flavors, Life Science Chromatograms FAME Standard 1. Butyric acid methyl ester (C4:0) Column: DB-WAX 10 m x 0.10 mm I.D., 0.10 µm P/N: Carrier: Hydrogen at 77 cm/sec, measured at 40 C Oven: 40 C for 0.5 min C at 25 /min C at 3 /min C at 8 /min 230 C for 1 min Injector: Split 1:30, 250 C Detector: FID, 250 C Caproic acid methyl ester (C6:0) 3. Caprylic acid methyl ester (C8:0) 4. Capric acid methyl ester (C10:0) 5. Undecanoic acid methyl ester (C11:0) 6. Lauric acid methyl ester (C12:0) 7. Tridecanoic acid methyl ester (C13:0) 8. Myristic acid methyl ester (C14:0) 9. Myristoleic acid methyl ester (C14:1) 10. Pentadecanoic acid methyl ester (C15:0) 11. cis-10-pentadecenoic acid methyl ester (C15:1) 12. Palmitic acid methyl ester (C16:0) 13. Palmitoleic acid methyl ester (C16:1) 14. Heptadecanoic acid methyl ester (C17:0) 15. cis-10-heptadecenoic acid methyl ester (C17:1) 16. Stearic acid methyl ester (C18:0) , Oleic acid methyl ester (C18:1n9c) 18. Elaidic acid methyl ester (C18:1n9t) 19. Linoleic acid methyl ester (C18:2n6c) 20. Linolelaidic acid methyl ester (C18:2n6t) 21. γ-linolenic acid methyl ester (C18:3n6) 22. Linolenic acid methyl ester (C18:3n3) 23. Arachidic acid methyl ester (C20:0) 24. cis-11-eicosenoic acid methyl ester (C20:1) 25. cis-11,14-eicosadienoic acid methyl ester (C20:2) 26. cis-8,11,14-eicosatrienoic acid methyl ester (C20:3n6) 27. Heneicosanoic acid methyl ester (C21:0) 28. cis-11,14,17-eicosatrienoic acid methyl ester (C20:3n3) 29. Arachidonic acid methyl ester (C20:4n6) 30. cis-5,8,11,14,17-eicosapentaenoic acid methyl ester (C20:5n3) 31. Behenic acid methyl ester (C22:0) 32. Erucic acid methyl ester (C22:1n9) 33. cis-13,16-docosadienoic acid methyl ester (C22:2) 34. Tricosanoic acid methyl ester (C23:0) 35. Lignoceric acid methyl ester (C24:0) 36. cis-4,7,10,13,16,19-docosahexaenoic acid methyl ester (C22:6n3) 37. Nervonic acid methyl ester (C24:1) 23 Excellent Resolution in 12 Minutes! , C583 FAME Standard Column: DB m x 0.10 mm I.D., 0.10 µm P/N: (cut to 10 m) Carrier: Hydrogen at 59.3 cm/sec, measured at 35 C Oven: 35 for 0.5 min C at 25 /min C at 3 /min C at 8 /min 230 C for 1 min Injector: Split 1:30, 250 C Detector: FID, 250 C 1. Butyric acid methyl ester (C4:0) 2. Caproic acid methyl ester (C6:0) 3. Caprylic acid methyl ester (C8:0) 4. Capric acid methyl ester (C10:0) 5. Undecanoic acid methyl ester (C11:0) 6. Lauric acid methyl ester (C12:0) 3 7. Tridecanoic acid methyl ester (C13:0) 8. Myristic acid methyl ester (C14:0) 9. Myristoleic acid methyl ester (C14:1) 10. Pentadecanoic acid methyl ester (C15:0) 11. cis-10-pentadecenoic acid methyl ester (C15:1) 12. Palmitic acid methyl ester (C16:0) 13. Palmitoleic acid methyl ester (C16:1) 14. Heptadecanoic acid methyl ester (C17:0) 15. cis-10-heptadecenoic acid methyl ester (C17:1) 16. Stearic acid methyl ester (C18:0) 17. Oleic acid methyl ester (C18:1n9c) Elaidic acid methyl ester (C18:1n9t) 19. Linoleic acid methyl ester (C18:2n6c) 20. Linolelaidic acid methyl ester (C18:2n6t) 21. γ-linolenic acid methyl ester (C18:3n6) 22. Linolenic acid methyl ester (C18:3n3) 23. Arachidic acid methyl ester (C20:0) 24. cis-11-eicosenoic acid methyl ester (C20:1) 25. cis-11,14-eicosadienoic acid methyl ester (C20:2) 26. cis-8,11,14-eicosatrienoic acid methyl ester (C20:3n6) 27. Heneicosanoic acid methyl ester (C21:0) 28. cis-11,14,17-eicosatrienoic acid methyl ester (C20:3n3) 29. Arachidonic acid methyl ester (C20:4n6) 30. cis-5,8,11,14,17-eicosapentaenoic acid methyl ester(c20:5n3) 31. Behenic acid methyl ester (C22:0) 32. Erucic acid methyl ester (C22:1n9) 33. cis-13,16-docosadienoic acid methyl ester (C22:2) 34. Tricosanoic acid methyl ester (C23:0) 35. Lignoceric acid methyl ester (C24:0) 36. cis-4,7,10,13,16,19-docosahexaenoic acid methyl ester (C22:6n3) 37. Nervonic acid methyl ester (C24:1) Faster Analysis Time With 0.10 mm I.D. Columns! C v priced-ap.p /24/01, 3:02 PM

76 Chromatograms Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions you need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: (USA & Canada) Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Food, Flavors, Life Science Eberhardt Kuhn, Sr. Technical Support Engineer FAMEs Column: DB-225ms 30 m x 0.25 mm I.D., 0.25 P/N: Carrier: Hydrogen at 40 cm/sec Oven: 200 C for 1 min C at 3 /min Injector: Split 1:50, 250 C Detector: FID Nitrogen makeup gas at 30 ml/min 16:0 18:0 20:0 22:0 24:0 14:0 28:0 30:0 26:0 31: The higher isothermal upper temperature limit of DB-225ms (260 C vs. 220 C for DB-225) allows the elution of higher molecular weight FAMEs (above 24:0) while maintaining a reasonable run time. C v priced-ap.p /14/01, 2:01 PM

77 Food, Flavors, Life Science Chromatograms Organic Acids Column: DB-FFAP 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 40 cm/sec, measured at 100 C Oven: 100 C for 5 min C at 10 /min 250 C for 12 min Injector: Split 1:50, 250 C Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min 1, Acetone 2. Formic acid 3. Acetic acid 4. Propionic acid 5. Isobutyric acid 6. Butyric acid 7. Isovaleric acid 8. Valeric acid (pentanoic acid) 9. Isocaproic acid 10. Caproic acid (hexanoic acid) Heptanoic acid 12. Octanoic acid 13. Decanoic acid 14. Dodecanoic acid 15. Tetradecanoic acid 16. Hexadecanoic acid 17. Octadecanoic acid 18. Arachidic acid (eicosanoic acid) 18 C min Butter Triglycerides Column: Peak Identification: P=Palmitic acid, hexadecanoic acid, C16:0 S=Stearic acid, octadecanoic acid, C18:0 O=Oleic acid, cis-9-octadecanoic acid, C18:1 TMS Sugars Column: DB-17ht DB m x 0.32 mm I.D., 0.15 µm 30 m x 0.25 mm I.D., 0.25 µm P/N: P/N: Carrier: Hydrogen at 40 cm/sec Carrier: Hydrogen at 40 cm/sec Oven: C at 5 /min Oven: 180 C for 2 min 365 C for 1 min C at 5 /min Injector: Cool On-column Injector: Split 1:50, 225 C 1 µl of 9 µg/µl in toluene Detector: FID, 250 C (approx. 1% w/w solution) Nitrogen makeup Detector: FID, 400 C gas at 30 ml/min Nitrogen makeup gas at 30 ml/min Baseline Corrected 1. D-Arabinose 3 2. D-Ribose anomers 3. D-Zylose 4. D-Mannose 5. D-Fructose 6. α-d-galactose 7. α-d-glucose 8. D-Fructose (anomer) 9. β-d-glucose PPP PPO Cholesterol PPS PSO POO PSS SSO SOO SSS OOO C212 C086 9 min 76 v priced-ap.p /24/01, 3:03 PM

78 Chromatograms Alditol Acetates Column: DB m x 0.25 mm I.D., 0.15 µm P/N: Carrier: Hydrogen at 36.5 cm/sec Oven: 220 C isothermal Injector: Split 1:50, 1 µl, 225 C Detector: FID, 250 C Nitrogen makeup gas at 30 ml/min 1 1. Rhamnitol 2. Fucitol 3. Ribitol 4. Arabinitol 5. Mannitol 6. Galactitol 7. Glucitol 8. Inositol Food, Flavors, Life Science C min Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions and savvy you need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: (USA & Canada) Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Cameron George, Sr. Technical Support Engineer 77 v priced-ap.p /14/01, 2:00 PM

79 Food, Flavors, Life Science Chromatograms Fragrance Reference Standard Conditions and compound list for all chromatograms: Column: DB-1 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 25 cm/sec, measured at 150 C Oven: 40 C for 1 min C at 5 /min Injector: Split 1:50, 250 C 1 µl of a 1:20 dilution of neat sample in acetone Detector: MSD, 300 C transfer line Many thanks to Carl Frey, Manager of Analytical Services, Dragoco, and Kevin Myung, Director of Flavor and Perfumery Research, Bush Boake Allen, Inc. for contributing to this work. 1. Acetone 2. 2,3-Butanedione (diacetyl) 3. Ethyl acetate 4. 2,3-Pentanedione (acetyl propionyl) 5. Ethyl propionate 6. Methyl butyrate 7. 3-Methylbutyl alcohol 8. 2-Methylbutyl alcohol 9. Isobutyl acetate 10. Ethyl butyrate 11. Furfural 12. Ethyl isovalerate 13. Hexanol 14. Allyl butyrate 15. Ethyl pentanoate 16. Hexylene glycol 17. α-thujone 18. Benzaldehyde 19. α-pinene 20. Camphene 21. 3,5,5-Trimethylhexanol 22. Sabinene 23. β-pinene 24. Ethyl hexanoate 25. Myrcene 26. Hexyl acetate 26a. cis-linalool oxide 26b. Methyl benzoate 26c. trans-linalool oxide 28. Methyl-cresol 29. Benzyl alcohol 30. para-cymene 31. 1,8-Cineol 32. Limonene 33. 2,6-Dimethylhept-5-enal 34. γ-terpinene 35. Octanol 37. Ethyl heptanoate 38. Linalool 39. Benzene ethanol 41. Rose oxide, cis-rose 42. Rose oxide, trans-rose 43. Camphor 44. Citronellal 45. Benzyl acetate 46. Menthone 47. Isoborneol 48. Isomenthone 49. Borneol 51. Terpinen-4-ol 52. α-terpineol 53. Ethyl octanoate 54. Octyl acetate 56. Fenchyl acetate 57. Citronellol 58. Neral 59. Carvonel 59a. Phenylethyl acetate 60. Geraniol 61. Linalyl acetate 62. Geranial 63. Hydroxycitronellal 64. Citronellyl formate 66. Bornyl acetate 67. Vertenex (isomer 1) 68. Ethyl nonanoate 69. Geranyl formate 70. Vertenex (isomer 2) 71. γ-nonalactone 72. Citronellyl acetate 73. Neryl acetate 74. Geranyl acetate 76. Diphenyl oxide 78. Ethyl decanoate 79. α-copaene 80. Florazone (isomer 1) 81. Florazone (isomer 2) 82. β-caryophyllene 83. Citronellyl propionate 85. 3,7-Guaiadiene 88. Dodecanol 89. Ethyl undecanoate 90. Eugenyl acetate 91. Frambione (raspberry ketone) 93. Isoamyl salicylate 94. δ-cadinene 95. cis-nerolidol 96. Rosatol (rosetone) 96a. Geranyl butyrate 97. trans-nerolidol 98. n-amyl salicylate 99. Phenylethyl tiglate 100. Ethyl dodecanoate 101. Benzophenone 102. Dibenzyl ether 103. γ-dodecalactone 104. Citronellyl tiglate 105. Evernyl 106. Geranyl tiglate 107. Geranyl-2-methyl valerate 108. Celestocide 109. Heptadec-1-ene 110. Benzyl benzoate 111. Ethyl tetradecanoate 112. Benzyl salicylate 113. Tonalid TM 114. Nonadec-1-ene 115. Isopropylmyristate 116. Ethyl pentadecanoate 116a. Nonadecane 117. Ethyl hexadecanoate 118. Musk T (ethylene brassylate) 119. Eicosane 120. Cinnamyl phenyl acetate 121. Heneicosane 122. Phenyl ethyl cinnamate 123. Ethyl octadecanoate 124. Hercolyn TM D (tetrahydro & dihydro methyl abietate) 125. Cinnamyl cinnamate 126. Cetearyl octanoate 127. Cetearyl decanoate , , , C389 made by sweet smelling elves 78 v priced-ap.p /24/01, 3:04 PM

80 Chromatograms Bergamot Oil , Geranium Oil Rosemary Oil Lavender Oil ,62 Food, Flavors, Life Science c 26a a Rose Oil Ylang Ylang Oil 45 38, a 28 26b?? a v priced-ap.p /24/01, 3:05 PM

81 Food, Flavors, Life Science Chromatograms Fragrance Reference Standard Conditions and compound list for all chromatograms: Column: DB-WAX 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 25 cm/sec, measured at 150 C Oven: 45 C for 2 min C at 3 /min 250 C for 34 min Injector: Split 1:50, 250 C 1 µl of a 1:20 dilution of neat sample in acetone Detector: MSD, 250 C transfer line Many thanks to Carl Frey, Manager of Analytical Services, Dragoco, and Kevin Myung, Director of Flavor and Perfumery Research, Bush Boake Allen, Inc. for contributing to this work. 1. Acetone 2. Ethyl acetate 3. Ethyl propionate 4. 2,3-Butanedione (diacetyl) 5. Methyl butyrate 6. Isobutyl acetate 7. α-pinene 8. Ethyl butyrate 9. 2,3-Pentanedione (acetyl propionyl) 10. Camphene 11. Ethyl isovalerate 12. β-pinene 13. Ethyl pentanoate 14. Myrcene 15. Allyl butyrate 16. Limonene 17. 1,8-Cineol 18. 3,5,5-Trimethylhexanol Methylbutyl alcohol Methylbutyl alcohol 21. Ethyl hexanoate 22. γ-terpinene 23. p-cymene 24. Hexyl acetate 25. Terpinolene 26. Ethyl heptanoate 27. 2,6-Dimethylhept-5-enal (Melonal TM ) 28. Rose oxide, cis-rose 29. Hexanol 30. Rose oxide, trans-rose 31. Methyl-para-cresol 32. Ethyl octanoate 33. cis-linalool oxide 34. Menthone 35. Furfural 36. trans-linalool oxide 37. Octyl acetate 38. Isomenthone 39. α-copaene 40. Camphor 41. Benzaldehyde 42. Ethyl nonanoate 43. Linalool 44. Linalyl acetate 45. Vertenex (isomer 1) 46. Octanol 47. β-caryophyllene 48. Vertenex (isomer 2) 49. Terpinen-4-ol 50. Methyl benzoate 51. Hexylene glycol 52. Ethyl decanoate 53. Citronellyl acetate 54. Isoborneol 55. Neral 56. α-terpineol 57. Geranyl formate 58. Borneol 59. β-bisabolene 60. Benzyl acetate 61. Neryl acetate 62. Geranial 63. Ethyl undecanoate 64. δ-cadinene 65. Geranyl acetate 66. Citronellol 67. Ethyl dodecanoate 68. Geraniol 69. Benzyl alcohol 70. Geranyl butyrate 71. Nonadecane 72. Benzene ethanol 73. Nonadec-1-ene 74. Florazone (isomer 1) 75. Florazone (isomer 2) 76. Hydroxycitronellal 77. Dodecanol 78. Diphenyl oxide 79. Citronellyl tiglate 80. Eugenyl methyl ether 81. γ-nonalactone 83. Ethyl tetradecanoate 84. n-amyl salicylate 85. Geranyl tiglate 86. Ethyl pentadecanoate 87. Isopropylmyristate 90. Phenylethyl tiglate 91. Rosatol (rosetone) 92. Eugenyl acetate 93. Ethyl hexadecanoate 94. γ-dodecalactone 95. Dibenzyl ether 96. Tonalid 97. Ethyl octadecanoate 98. Benzophenone 99. Benzyl benzoate 100. Cetearyl octanoate 101. Musk T (ethylene brassylate) 102. Cetearyl decanoate 103. Frambione (raspberry ketone) 104. Cinnamyl phenyl acetate 105. Phenyl ethyl cinnamate 106. Cinnamyl cinnamate 107. Benzyl salicylate 108. Heneicosane 109. Sabinene 110. Citronellyl formate ,28 19, , , , C v priced-ap.p /24/01, 3:05 PM

82 Chromatograms Bergamot Oil Geranium Oil Rosemary Oil Lavender Oil Food, Flavors, Life Science Rose Oil Ylang Ylang Oil v priced-ap.p /24/01, 3:07 PM

83 Food, Flavors, Life Science Chromatograms Turpentine Oil Column: DB-WAX P/N: m x 0.25 mm I.D. 0., 0.5 µm 1 Carrier: Hydrogen at 45 cm/sec measured at 70 C Oven: C at 3 /min Injector: Split 1:150, 220 C Detector: FID, 220 C 1. α-pinene 2. Camphene 3. β-pinene 4. 3-Carene 5. α-phellandrene 6. α-terpinene 7. Limonene 8. β-phellandrene 9. γ-terpinene 10. p-cymene 11. Terpinolene 12. Caryophyllene 13. Terpinen-4-ol 14. α-terpineol C Lemon Oil Column: DB-5 20 m x 0.10 mm I.D., 0.1 µm P/N: Carrier: Hydrogen at 60 cm/sec, measured at 40 C Oven: 40 C for 3 min C at 30 /min 185 C for 3 min Injector: Split 1:275, 275 C Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min 1. α-thujone 2. β-thujone 3. Camphene 4. Sabinene 5. β-pinene 6. Myrcene 7. Octanal 8. α-phellandrene 9. α-terpinene 10. p-cymene 11. δ-limonene 12. γ-terpinene 13. Octanol 14. Terpinolene 15. Linalool 16. Nonanal 17. Citronellal 18. Terpinen-4-ol 19. α-terpineol 20. Decanal 21. Neral 22. Geranial 23. Neryl acetate 24. Geranyl acetate 25. β-caryophyllene 26. trans-α-bergamotene 27. α-humulene 28. β-bisabolene Faster Analysis Time With 0.1 mm I.D. Columns! C v priced-ap.p /24/01, 3:07 PM

84 { Chromatograms Citrus Flavored Carbonated Beverage (Soda) Column: CycloSil-B 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 37 cm/sec, measured at 40 C Oven: C at 2 /min Detector: HP 5973 MSD, 280 C transfer line SPME Fiber: Polyacrylate fiber, 85 µm Extraction: Headspace, no stir, NaCl 1g/10 ml sample 68 min at 27 C adsorption Desorption: 250 C for 15 min, Split 1: S(-)Limonene 2. p-cymene 3. R(+)Limonene 4. Octanol 5. γ-terpinene 6. Nonanol 7. 2-Ethyl-1-Hexanol 8. Linalool 9. Decanol 10. Terpinen-4-ol 11. Phenethylalcohol 12. α-terpineol 13. BHT Food, Flavors, Life Science C Rosemary Oil Column: CycloSil-B 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Hydrogen at 40 cm/sec, measured at 60 C Oven: 55 C for 1 min C at 5 /min Injector: Split 1:50, 250 C Detector: FID, 340 C 1 1. (+/-) α-pinene 2. (+/-) Camphene 3. (+/-) β-pinene 4. (+/-) Limonene 5. (+/-) Linalool 6. (+/-) Camphor 7. (+/-) Terpinene-4-ol 8. (+/-) Isoborneol 9. (+/-) Borneol 10. (+/-) α-terpineol C TIme (min) 83 v priced-ap.p /24/01, 3:07 PM

85 Food, Flavors, Life Science Chromatograms Chiral Standards Column: Cyclodex-B 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 35 cm/sec, measured at 60 C Oven: 60 C for 5 min C at 3 /min Injector: Split 1:250, 250 C Detector: FID, 250 C Nitrogen makeup gas at 30 ml/min Bromopentane 2. α-pinene 3. 2,3-Butanediol 4. γ-valerolactone 5. Methylbenzylamine 6. sec-phenylethyl alcohol 7. 2-Ethylhexanoic acid C Menthol Column: Cyclodex-B 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Hydrogen at 55 cm/sec Oven: 105 C isothermal Injector: Split 1:100, 250 C 1 µl of 1 µg/µl each in chloroform Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min 1. (+)-Neomenthol 2. (-)-Neomenthol 3. (+)-Menthol 4. (-)-Menthol C min 84 v priced-ap.p /24/01, 3:08 PM

86 Chromatograms Residual Solvents, USP <467> Column: DB m x 0.53 mm I.D., 3.0 µm P/N: Carrier: Helium at 35 cm/sec, measured at 40 C Oven: 40 C for 20 min C at 5 /min Injector: Megabore Direct, 250 C 5 m phenylmethylsiloxane deactivated retention gap Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min ,14, Methanol 2. Ethanol 3. Ethyl ether 4. Acetone 5. Isopropanol 6. Acetonitrile 7. Methylene chloride 8. tert-butanol 9. n-hexane 10. n-propanol 11. Methyl ethyl ketone (MEK) 12. Ethyl acetate 13. Tetrahydrofuran (THF) 14. Chloroform 15. sec-butanol 16. Cyclohexane 17. Benzene 18. n-heptane 19. Trichloroethylene 20. n-butanol 21. 1,4-Dioxane 22. Pyridine 23. Toluene 24. Dimethylformamide (DMF) Food, Flavors, Life Science min C144 Over-the-Counter Pain Killers - TMS Derivatives Column: DB-5 20 m x 0.18 mm I.D., 0.4 µm P/N: Carrier: Helium at 39 cm/sec, measured at 100 C Oven: C at 10 /min Injector: Split 1:100, 250 C 1 µl of 2 µg/µl each in pyridine Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min 1. Nicotine 2. Unknown 3. Acetylsalicylic acid (aspirin) 4. Ibuprofen 5. Acetaminophen 1 6. Unknown 7. Caffeine min C v priced-ap.p /24/01, 3:08 PM

87 Food, Flavors, Life Science Chromatograms Blood Alcohols (Static Headspace/Split) Column: DB-ALC1 30 m x 0.53 mm I.D., 3.0 µm P/N: Carrier: Helium at 80 cm/sec, measured at 40 C Oven: 40 C Isothermal Injector: Headspace to split inlet, Split 1:10, 250 C Detector: FID, 300 C Nitrogen makeup gas at 23 ml/min 1. Methanol 2. Acetaldehyde 3. Ethanol 4. Isopropanol 5. Acetone 6. 1-Propanol 3 4 Headspace Conditions Oven: 70 C Loop: 80 C Transfer Line: 90 C Vial Equil. Time: 10 min Pressurization Time: 0.20 min Loop Fill Time: 0.20 min Loop Equil. Time: 0.05 min Inject Time: min Sample Loop Size: 1.0 ml Sample Composition: 0.1% Ethanol, 0.001% Others Blood Alcohols (Static Headspace/Split) Column: DB-ALC2 30 m x 0.53 mm I.D., 2.0 µm P/N: Carrier: Helium at 80 cm/sec, measured at 40 C Oven: 40 C Isothermal Injector: Headspace to split inlet, Split 1:10, 250 C Detector: FID, 300 C Nitrogen makeup gas at 23 ml/min 3 1. Methanol 2. Acetaldehyde 3. Ethanol 4 4. Isopropanol 5. Acetone 6. 1-Propanol Headspace Conditions Oven: 70 C Loop: 80 C Transfer Line: 90 C Vial Equil. Time: 10 min Pressurization Time: 0.20 min Loop Fill Time: 0.20 min Loop Equil. Time: 0.05 min Inject Time: min Sample Loop Size: 1.0 ml Sample Composition: 0.1% Ethanol, 0.001% Others C C671 Drug Screen Column: DB-1ms 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 40 cm/sec, measured at 50 C Oven: 50 C for 1.0 min C at 25 /min C at 10 /min 325 C for 5 min Injector: Cold Splitless using Optic II injector C at 10 /sec, 45 sec purge activation time Sample: 1 µl injection of ppm standard Detector: FID, 300 C Nicotine 2. Caffeine 3. Glutethimide 4. Lidocaine 5. PCP 6. Phenobarbital 7. Methadone primary metabolite 8. Methaqualone 9. Methadone 10. Cocaine 11. Desipramine 12. Carbazepine 13. Trimipramine 14. Heroin 15. Fentanyl 16. Ibogaine 17. Triazolam 18. LSD C v priced-ap.p /24/01, 3:08 PM

88 Chromatograms Hallucinogens Column: P/N: DB-17ms 30 m x 0.25 mm I.D., 0.25 µm Carrier: Helium at 30 cm/sec, measured at 50 C Oven: 50 C for 0.5 min C at 25 /min C at 10 /min C at 25 /min 320 C for 16 min Injector: Splitless, 250 C 30 sec purge activation time 1 µl of ng/µl standard in methanol Detector: MSD, 300 C transfer line full scan at m/z Methylaminorex 2. Mescaline 3. Dimethyltryptamine 4. Ketimine 5. TCP (Tenocyclidine) 6. PCP 7. Diethyltryptamine 8. Bufotenine 9. Fentanyl 10. Ibogaine 11. LSD 12. Psilocin (peak not shown) 11 Food, Flavors, Life Science C468 Drugs Column: DB-5 15 m x 0.32 mm I.D., 0.25 µm 10 m Duraguard TM Column P/N: Carrier: Helium at 35.3 cm/sec, measured at 50 C Oven: C at 10 /min Injector: Splitless, 250 C 30 sec purge activation time Detector: FID, 300 C Butabarbital 2. Talbutal 3. Mephenytoin 4. Thiopental 5. PCP 6. Procaine 7. Trimipramine 8. Imipramine 9. Codeine 10. Lorazepam 11. Diazepam 12. Desmethyldiazepam 13. Flunitrazepam 14. Bromazepam C v priced-ap.p /24/01, 3:08 PM

89 Petroleum & Industrial Chemicals Chromatograms Alcohols Column: DB-PS1 30 m x 0.53 mm I.D., 3.0 µm P/N: Carrier: , CS Helium at 30 cm/sec, measured at 40 C Oven: 40 C for 5 min C at 10 /min 230 C for 3 min Injector: Split 1:10, 250 C 1 µl of % each solvent in CS 2 Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min , ,17 18,19, Methanol 2. Ethanol 3. Isopropanol 4. tert-butanol 5. 2-Propen-1-ol (allyl alcohol) 6. 1-Propanol 7. 2-Propyn-1-ol (propargyl alcohol) 8. sec-butanol 9. 2-Methyl-3-buten-2-ol 10. Isobutanol Methoxyethanol (methyl Cellosolve) Buten-1-ol Methyl-2-butanol (tert-amyl alcohol) 26, C , Butanol Buten-1-ol (crotyl alcohol) 16. Ethylene glycol Penten-3-ol Pentanol 19. Glycidol Pentanol Ethoxyethanol (Cellosolve) 22. Propylene glycol Methyl-1-butanol (isoamyl alcohol) Methyl-1-butanol (active amyl alcohol) Methyl-2-pentanol Pentanol Penten-1-ol Methyl-2-buten-1-ol 29. Cyclopentanol 42, , Hexanol Hexanol Hydroxy-4-methyl -2-pentanone 33. Furfuryl alcohol 34. cis-3-hexen-1-ol Hexanol 36. cis-2-hexen-1-ol 37. Cyclohexanol Heptanol Heptanol Butoxyethanol (butyl Cellosolve) 41. cis-4-hepten-1-ol 42. trans-2-hepten-1-ol Heptanol 44. Benzyl alcohol Ethyl-1-hexanol 46. a-methylphenyl alcohol Octanol Nonanol Phenoxyethanol 50. a-ethylphenethyl alcohol 51. b-ethylphenethyl alcohol Decanol Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions you need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: (USA & Canada) Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Angelica Reese, Technical Support Engineer 88 v priced-ap.p /14/01, 2:04 PM

90 Chromatograms Alcohols Column: DB-PSWAX 30 m x 0.53 mm I.D., 1.0 µm P/N: Carrier: Helium at 30 cm/sec, measured at 40 C Oven: 40 C for 5 min C at 10 /min 230 C for 3 min Injector: Split 1:10, 250 C 1 µl of % each solvent in CS 2 Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min 1. Methanol 2. Ethanol 3. Isopropanol 4. tert-butanol 5. 2-Propen-1-ol (allyl alcohol) 6. 1-Propanol 7. 2-Propyn-1-ol (propargyl alcohol) 8. sec-butanol 9. 2-Methyl-3-buten-2-ol 10. Isobutanol Methoxyethanol (methyl Cellosolve) Buten-1-ol Methyl-2-butanol (tert-amyl alcohol) Butanol Buten-1-ol (crotyl alcohol) 16. Ethylene glycol Penten-3-ol Pentanol 19. Glycidol Pentanol Ethoxyethanol (Cellosolve) 22. Propylene glycol Methyl-1-butanol (isoamyl alcohol) Methyl-1-butanol (active amyl alcohol) Methyl-2-pentanol Pentanol Penten-1-ol Methyl-2-buten-1-ol 29. Cyclopentanol Hexanol Hexanol Hydroxy-4-methyl-2-pentanone 33. Furfuryl alcohol 34. cis-3-hexen-1-ol Hexanol 36. cis-2-hexen-1-ol 37. Cyclohexanol Heptanol Heptanol Butoxyethanol (butyl Cellosolve) 41. cis-4-hepten-1-ol 42. trans-2-hepten-1-ol Heptanol 44. Benzyl alcohol Ethyl-1-hexanol 46. α-methylphenyl alcohol Octanol Nonanol Phenoxyethanol 50. α-ethylphenethyl alcohol 51. β-ethylphenethyl alcohol Decanol Petroleum & Industrial Chemicals C v priced-ap.p /27/01, 10:34 AM

91 Petroleum & Industrial Chemicals Chromatograms Acrylate Impurities Column: DB m x 0.53 mm I.D., 1.0 µm P/N: Carrier: Helium at 34.5 cm/sec measured at 35 C Oven: 35 C for 5 min, C at 10 /min Injector: Split 1:10, 230 C Detector: FID, 250 C 1 * 2 3 * * 6 * 11 * 9 * 1. Methanol 2. Ethanol 3. Methyl acetate 4. Ethyl acetate 5. Methyl acrylate 6. Methyl propionate 7. Isobutanol 8. Butanol 9. Ethyl acrylate 10. Ethyl propionate 11. Methyl methacrylate 12. Isopropyl acrylate 13. Isobutyl acetate 14. Butyl acetate 15. Isobutyl acrylate 16. Dibutyl ether 17. Isobutyl propionate 18. Butyl acrylate 19. Butyl propionate * Methyl acrylate impurities Ethyl acrylate impurities Butyl acrylate impurities C Acrylate Impurities Column: DB-PS m x 0.53 mm I.D., 1.0 µm P/N: Carrier: Helium at 36.8 cm/sec measured at 35 C Oven: 35 C for 5 min, C at 10 /min Injector: Split 1:10, 230 C Detector: FID, 250 C 1 * 2 3* 4 5 * 6 * 7 10,11 * 9 * Methanol 2. Ethanol 3. Methyl acetate 4. Ethyl acetate 5. Methyl acrylate 6. Methyl propionate 7. Isobutanol 8. Butanol 9. Ethyl acrylate 10. Ethyl propionate 11. Methyl methacrylate 12. Isopropyl acrylate 13. Isobutyl acetate 14. Butyl acetate 15. Isobutyl acrylate 16. Dibutyl ether 17. Isobutyl propionate 18. Butyl acrylate 19. Butyl propionate * Methyl acrylate impurities Ethyl acrylate impurities Butyl acrylate impurities C Organic Acids Column: DB-WAXetr 30 m x 0.53 mm I.D., 1.0 µm P/N: Carrier: Helium at 37 cm/sec, measured at 40 C Oven: 125 C for 5 min C at 15 /min 180 C for 12 min Injector: Split, 250 C Detector: FID, 250 C Acetic acid 2. Propionic acid 3. Isobutyric acid 4. Butyric acid 5. Isovaleric acid 6. Valeric acid (pentanoic acid) 7. Isocaproic acid 8. Caproic acid (hexanoic acid) 9. Heptanoic acid C v priced-ap.p /24/01, 3:12 PM

92 Chromatograms Amines in Water Column: CAM 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Hydrogen at 38 cm/sec Oven: C at 10 /min Injector: Split Detector: FID Nitrogen makeup gas at 30 ml/min 1 C Ethylenediamine 2. Piperazine 3. Diethylenetriamine 4. N-(2-Aminoethyl)piperazine 5. Aminoethylethanolamine 6. Triethylenetetramine (4 isomers) 7. Tetraethylenepentamine (4 isomers) min 7 Primary Amines C Column: CAM 30 m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Hydrogen at 40 cm/sec Oven: 110 C isothermal Injector: Split Detector: FID Nitrogen makeup gas at 30 ml/min 1. n-octylamine 2. n-nonylamine 3. n-decylamine 4. Benzylamine 5. Dicyclohexylamine 3 14 min 4 5 Petroleum & Industrial Chemicals C399 Amines and Nitriles Column: DB-5ms 30 m x 0.25 mm I.D., 0.5 µm P/N: Carrier: Helium at 22 cm/sec, measured at 40 C Oven: 40 C for 1 min C at 10 /min Injector: Split 1:50, 250 C 1 µl of 100 ng/µl standard in methanol Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min 1. Diethylamine 2. Propionitrile 3. Diisopropylamine 4. Triethylamine 5. Pyridine 6. Pyrimidine 7. Pyrazole 8. Acrylamide 9. Pyridazine 10. Aniline Bromopyridine 12. Benzonitrile Cyanopyridine 14. Benzylamine 15. n-octylamine Methyl-2-pyrrolidine 17. N,N-Dimethylbenzylamine 18. Phenylethylamine 19. N-Benzylmethylamine Cyanopyridine Chloroaniline 22. n-nonylamine 23. 2,4-Dimethylaniline Chlorobenzonitrile 25. 2,6-Dimethylaniline Chloroaniline Chloroaniline 28. N,N-Diethylaniline 29. n-decylamine Bromoaniline 31. 3,4-Diaminotoluene 32. 2,6-Diethylaniline Nitroaniline 34. Dicyclohexylamine 35. 3,4-Dichloroaniline Nitroaniline Nitroaniline 38. Diphenylaniline 1, , , made by an elf who said it was "amines to an end" 91 v priced-ap.p /24/01, 3:12 PM

93 Petroleum & Industrial Chemicals Chromatograms C590 Impurities in Mixed Xylenes Column: DB-WAXetr 60 m x 0.32 mm I.D., 0.25 µm P/N: Carrier: Helium at 20 cm/sec, measured at 145 C Oven: 60 C for 10 min C at 5 /min 150 C for 10 min Injector: Split 1:150, 230 C Detector: FID, 240 C 1. Benzene 2. Toluene 3. Ethylbenzene 4. p-xylene 5. m-xylene 6. o-xylene 7. n-butylbenzene (IS) IS - Internal Standard non-aromatics ASTM D C9 aromatics 7 Organic Acids Column: DB-WAXetr 30 m x 0.53 mm I.D., 1.0 µm P/N: Carrier: Helium at 37 cm/sec, measured at 40 C Oven: 125 C for 5 min C at 15 /min 180 C for 12 min Injector: Split, 250 C Detector: FID, 250 C 1. Acetic acid 2. Propionic acid 3. Isobutyric acid 4. Butyric acid 5. Isovaleric acid 6. Valeric acid (pentanoic acid) 7. Isocaproic acid 8. Caproic acid (hexanoic acid) 9. Heptanoic acid C Aromatic Solvents Column: DB m x 0.25 mm I.D., 0.25 µm P/N: Carrier: Helium at 31 cm/sec Oven: 50 C for 5 min C at 10 /min Injector: Split 1:100, 250 C 0.5 µl of 0.5 µg/µl standard in hexane Detector: FID, 300 C Nitrogen makeup gas at 30 ml/min Benzene 2. Toluene 3. Ethylbenzene 4. Chlorobenzene 5. p-xylene 6. m-xylene 7. o-xylene 8. Styrene 9. Isopropylbenzene 10. n-propylbenzene Chlorotoluene Chlorotoluene Chlorotoluene 14. tert-butylbenzene 15. sec-butylbenzene 16. Isobutylbenzene 17. 1,3-Dichlorobenzene 18. 1,4-Dichlorobenzene 19. n-butylbenzene 20. 1,2-Dichlorobenzene 21. 1,3-Diisopropylbenzene 22. 1,4-Diisopropylbenzene Nitrotoluene Nitrotoluene Nitrotoluene C v priced-ap.p /24/01, 3:12 PM

94 Chromatograms Solvents Column: GS-CarbonPLOT 30 m x 0.32 mm I.D., 1.5 µm P/N: Carrier: Helium at 30 cm/sec measured at 125 C Oven: 125 C for 2 min C at 20 /min Injector: Split 1:20, 250 C Detector: MS, SIM mode 280 C transfer line 1. Methylene chloride 2. Acetone 3. Carbon disulfide 4. Ethyl ether 5. Ethyl acetate 6. Benzene C Petroleum & Industrial Chemicals Hydrocarbons Column: GS-CarbonPLOT 30 m x 0.32 mm I.D., 1.5 µm P/N: Carrier: Helium at 30 cm/sec measured at 125 C Oven: 125 C for 2 min C at 20 /min Injector: Split 1:20, 250 C Detector: MS, SIM mode 280 C transfer line 1. Methane 2. Acetylene 3. Ethylene 4. Ethane 5. Propyne 6. Propylene 7. Propane 8. Isobutane 9. Butane 10. Isopentane 11. Pentane C v priced-ap.p /24/01, 3:12 PM

95 Petroleum & Industrial Chemicals Chromatograms Volatile Sulfur Compounds Column: GS-GasPro 60 m x 0.32 mm I.D. P/N: Carrier: Helium at 10 psig (16 cm/sec, measured off COS at 80 C) Oven: 80 C for 2.5 min 80 to 260 C at 15 /min 260 C for 8 min Injector: Split 1:10, 200 C 1000 µl of sulfur gas standard, 3 ppmv each component Detector: PFPD (OI Analytical), 270 C J&W wishes to thank Air Toxics, Ltd. (Folsom, CA) for providing the standard mixture shown in this chromatogram. 1. Carbonyl sulfide 2. Hydrogen sulfide 3. Carbon disulfide 4. Methyl mercaptan 5. Ethyl mercaptan 6. Thiophene 7. Dimethyl sulfide 8. 2-Propanethiol 9. 1-Propanethiol Methyl-2-propanethiol Methyl-1-propanethiol 12. Ethyl methyl sulfide Butanethiol Methylthiophene 15. Methyl disulfide 16. Diethyl sulfide Ethylthiophene 18. 2,5-Dimethylthiophene 19. Tetrahydrothiophene 20. Ethyl disulfide C Volatile Sulfur Compounds Column: DB-1 30 m x 0.32 mm I.D., 5.0 µm P/N: Carrier: Helium at 23 cm/sec (H 2S at 50 C) Oven: 50 C for 4 min, C at 20 /min, 120 C for 4 min, C at 25 /min, 220 C for 2.5 min Injector: Split 1:10, 200 C, 600 µl of sulfur gas standard, 3 ppmv each component Detector: PFPD (OI Analytical), 220 C J&W wishes to thank Air Toxics, Ltd. (Folsom, CA) for providing the standard mixture shown in this chromatogram. 1. Hydrogen sulfide 2. Carbonyl sulfide 3. Methyl mercaptan 4. Ethyl mercaptan 5. Dimethyl sulfide 6. Carbon disulfide 7. 2-Propanethiol 8. 2-Methyl-2-propanethiol 9. 1-Propanethiol 10. Ethyl methyl sulfide 11. Thiophene Methyl-1-propanethiol 13. Diethyl sulfide Butanethiol 15. Methyl disulfide Methylthiophene Methylthiophene 18. Tetrahydrothiophene Pentanethiol Ethylthiophene 21. 2,5-Dimethylthiophene Hexanethiol 23. Ethyl disulfide Heptanethiol C v priced-ap.p /24/01, 3:13 PM

96 Chromatograms Mercaptans Column: 1 GS-GasPro 30 m x 0.32 mm I.D. P/N: Carrier: Helium at 42 ml/min Oven: 175 C for 2 min C at 10 /min 260 C Hold Injector: Split, 1: µl Detector: FID Ethyl mercaptan 2. 2-Propyl mercaptan 3. 1-Propyl mercaptan 4. 2-Methyl-2-propyl mercaptan 5. 2-Methyl-1-propyl mercaptan 6. 1-Methyl-1-propyl mercaptan 7. 1-Butyl mercaptan 8. 1-Pentyl mercaptan 9. 1-Hexyl mercaptan 8 9 Sulfur Compounds? No Problem. Petroleum & Industrial Chemicals 0 Time(min) 9 C530 Sulfur Gases in Light Hydrocarbon Stream Column: GS-GasPro 30 m x 0.32 mm I.D. P/N: Carrier: Helium at 53 cm/sec Oven: 25 C for 4 min C at 10 /min 200 C Hold Injector: 200 µl Detector: TCD Nitrogen 2. Methane 3. Ethane 4. CO 2 5. SF 6 6. Ethylene 7. COS 8. Acetylene 9. Propane 10. H 2 S 11. Ethylene oxide/cyclopropane 12. Propylene 13. Propadiene 14. Isobutane 15. Butane 16. SO Methyl acetylene C579 0 Time(min) v priced-ap.p /24/01, 3:13 PM

97 Petroleum & Industrial Chemicals Chromatograms Permanent Gases in Refinery Gas Column: GS-GasPro 60 m x 0.32 mm I.D. P/N: Carrier: Helium at 20 psig (constant pressure) Injector: Split 1:15, 200 C 100 µl, manual inject Detector: TCD, 250 C, background compensated Oven: -80 C for 3 min, -80 to 260 C at 25 /min, 260 C Hold 1. Hydrogen 2. Oxygen 3. Nitrogen 4. Carbon monoxide 5. Methane 6. Ethane 7. Carbon dioxide 8. Ethylene 9. Propane 10. Propylene 11. Isobutane 12. n-butane Butene 14. trans-2-butene 15. cis-2-butene 16. Isopentane 17. n-pentane C Permanent Gases Column: GS-CarbonPLOT 30 m x 0.32 mm I.D., 3.0 µm P/N: Carrier: Helium at 27 cm/sec (calculated off N 2 at 35 C) Oven: 35 C isothermal Injector: Split 1:30, 185 C, 250 µl injection volume Detector: TCD, 150 C 1. Nitrogen and Oxygen (5% each) 2. Carbon monoxide (5%) 3. Methane (4%) 4. Carbon dioxide (5%) C v priced-ap.p /24/01, 3:13 PM

98 Chromatograms Ozone Precursor Analysis on GS-Alumina and GS-GasPro Column: GS-Alumina 30 m x 0.53 mm I.D. P/N: Carrier: Helium at 52 cm/sec (6.7 ml/min), measured at 100 C Oven: 100 C for 1 min C at 8 /min 140 C for 0.5 min C at 30 /min Injector: Split 1:8, 250 C 300 µl injection of 100 ppm V SUMMA cannister mixture Detector: FID, 275 C Nitrogen makeup gas at 29 ml/min , 38, , 44, C608 Enlarged view of peaks Column: GS-GasPro 60 m x 0.32 mm I.D. 23, 28, 29 38, 39 P/N: Carrier: Helium at 40 cm/sec (3.3 ml/min), measured at 80 C Oven: 80 C for 0.5 min 20, C at 25 /min 175 C for 2 min C at 25 /min Injector: Split 1:17, 250 C 500 µl injection of 100 ppmv Detector: SUMMA cannister mixture FID, 275 C 24 33, Nitrogen makeup gas at 32 ml/min Enlarged view of peaks , 27, , (not all peaks shown ) 1. Methane 2. Ethane 3. Ethylene 4. Propane 5. Propylene 6. Isobutane 7. Acetylene 8. n-butane 9. trans-2-butene Butene 11. cis-2-butene 12. Cyclopentane 13. Isopentane 14. n-pentane 15. Propyne 16. 1,3-Butadiene 17. Cyclopentene Methyl-1-butene 19. trans-2-pentene Methyl-2-butene Pentene 22. cis-2-pentene 23. Methylcyclopentane 24. 2,2-Dimethylbutane 25. Cyclohexane 26. 2,3-Dimethylbutane Methylpentane Methylpentane 29. Isoprene 30. n-hexane Methyl-1-pentene 32. trans-2-hexene Methyl-1-pentene 34. cis-2-hexene 35. 2,4-Dimethylpentane 36. Methylcyclohexane 37. 2,3-Dimethylpentane Methylhexane Methylhexane 40. n-heptane 41. Benzene 42. Isooctane (2,2,4-trimethylpentane) 43. 2,3,4-Trimethylpentane Methylheptane Methylheptane 46. n-octane 47. Toluene 48. n-nonane 49. Ethylbenzene 50. m-xylene 51. p-xylene 52. o-xylene 53. Styrene 54. Isopropylbenzene (cumene) 55. n-propylbenzene 56. 1,3,5-Trimethylbenzene 57. 1,2,4-Trimethylbenzene Petroleum & Industrial Chemicals , C v priced-ap.p /24/01, 3:13 PM

99 Petroleum & Industrial Chemicals Chromatograms Ethylene Column: GS-Alumina 50 m x 0.53 mm I.D. P/N: Carrier: Helium at 11 ml/min, measured at 35 C Oven: 35 C for 2 min C at 6 /min 190 C for 3 min Injector: Split 1:30, 200 C 0.2 ml of trace hydrocarbons in ethylene Detector: FID, 200 C Nitrogen makeup gas at 20 ml/min 1. Methane 2. Ethane 3. Ethylene 4. Propane 5. Cyclopropane 6. Propylene 7. Isobutane 8. Acetylene 9. n-butane 10. Propadiene 11. trans-2-butene Butene 13. Isobutylene 14. cis-2-butene 15. Isopentane 16. n-pentane 17. Propyne 18. 1,3-Butadiene Pentene 20. n-hexane C Propylene Column: GS-Alumina 50 m x 0.53 mm I.D. P/N: Carrier: Helium at 10 ml/min, measured at 35 C Oven: 35 C for 2 min C at 10 /min 190 C for 3 min Injector: Split 1:30, 200 C 0.2 ml of trace hydrocarbons in propylene Detector: FID, 200 C Nitrogen makeup gas at 20 ml/min Methane 2. Ethane 3. Ethylene 4. Propane 5. Cyclopropane 6. Propylene 7. Isobutane 8. Acetylene 9. n-butane 10. Propadiene 11. trans-2-butene Butene 13. Isobutylene 14. cis-2-butene 15. Isopentane 16. n-pentane 17. Propyne 18. 1,3-Butadiene Pentene 20. n-hexane C v priced-ap.p /24/01, 3:14 PM

100 Chromatograms 1,3-Butadiene Column: DB m x 0.20 mm I.D., 1.12 µm P/N: Carrier: Helium at 1.0 ml/min Oven: -20 C for 3 min C at 4 /min C at 8 /min 200 C for 10 min Injector: Split 1:150, 250 C 0.5 µl injection Detector: FID, 250 C J&W wishes to thank DCG Industries (Pearland, TX) for providing this chromatogram. C , , Refined Butadiene Standard Component Gravimetric concentration (PPM) 1. Acetylene Propane Propylene Propadiene (allene) Propyne (methylacetylene) Cyclopropane Isobutane Butene Isobutylene n-butene ,3-Butadiene balance 12. trans-2-butene cis-2-butene Butyne (ethylacetylene) ,2-Butadiene Methyl-1-butene Isopentane Pentene n-pentane Butyne (dimethylacetylene) trans-2-pentene Isoprene cis-2-pentene trans-1,3-pentadiene cis-1,3-pentadiene Benzene Toluene Dimer (4-vinylcyclohexene-1) Petroleum & Industrial Chemicals Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions you need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: (USA & Canada) Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Jason Ellis, Technical Support Engineer 99 v priced-ap.p /14/01, 2:07 PM

101 Petroleum & Industrial Chemicals Chromatograms Regular Unleaded Gasoline (California Phase 1) - "Normal" GC Run Column: DB-Petro m x 0.25 mm I.D., 0.5 µm P/N: A6 Carrier: Hydrogen at 31 cm/sec Oven: 35 C for 9.5 min C at 13.3 /min 45 C for 11 min C at 1.4 /min 60 C for 11 min C at 2.7 /min 220 C for 3.6 min Injector: Split 1:200, 0.2 µl Detector: FID, 300 C enlarged view at min C Regular Unleaded Gasoline (California Phase 1) - "Fast" GC Run Column: Oven: DB-1 40 m x 0.10 mm I.D., 0.2 µm P/N: Carrier: Hydrogen at 34.8 cm/sec 35 C for 3.6 min C at 36.1 /min 45 C for 4.2 min C at 3.9 /min 60 C for 4.2 min C at 6.9 /min 220 C for 1.4 min Injector: Split 1:400, 0.2 µl Detector: FID, 300 C Compare Resolution enlarged view at min C v priced-ap.p /27/01, 10:40 AM

102 Chromatograms n-paraffin Standard Column: DB-HT Sim Dis 5 m x 0.53 mm I.D., 0.15 µm P/N: C Carrier: Helium at 18 ml/min, measured at 35 C Oven: C at 10 /min Injector: OPTIC TM PTV C at 2 /sec 0.5 µl of about 2% n-paraffins in CS 2 Detector: FID, 450 C Nitrogen makeup gas at 15 ml/min Peak numbers denote carbon number Petroleum & Industrial Chemicals Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: (USA & Canada) Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Cameron George, Sr. Technical Support Engineer 101 v priced-ap.p /14/01, 2:08 PM

103 GC Accessories Table of Contents Books Conversion Kits & Liners Ferrules Flowmeters Gas Purifiers - Moisture Traps O 2 /H 2 O Traps Oxygen Traps Carbon Dioxide Traps Hydrocarbon Traps High Pressure Traps Big Traps MegaSorp Point of Operation Panels Gas Handling Liners Septa Standards Tools Tubing - Fused Silica Tubing Accessories Unions - Zero Dead Volume Solid Phase Extraction www. v priced-ap.p /21/01, 5:58 PM

104 Books Agilent Technologies offers a variety of books for the gas chromatographer. All of these excellent references were written by Agilent authors. Both Analytical Gas Chromatography by Jennings, Mittlefehldt and Stremple and A Practical Guide to the Care, Maintenance and Troubleshooting of Capillary GC Systems by Rood are highly recommended. GC Reference Books Read by elves everywhere Description Part Number (P/N) Price ($) Analytical Gas Chromatography, 2nd Ed W.G. Jennings, E. Mittlefehldt, P. Stremple A Practical Guide to the Care, Maintenance and Troubleshooting of Capillary GC Systems, 3rd Ed. D. Rood Philip Stremple Walt Jennings Cofounder, J&W Scientific Dean Rood Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: (USA & Canada) Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Angelica Reese, Technical Support Engineer www. 103 v priced-ap.p /14/01, 2:09 PM

105 Conversion Kits & Liners Megabore Injection Port Liners for J&W Injection Port Conversion Kits We offer a wide variety of Megaboreliners. The Direct Injection Liner minimizes the potential for sample backflash, providing the most efficient transfer of the sample from the inlet into the column. With the Hot On-column Liner, the analyst can inject samples directly into the column with a standard 26-gauge syringe. The Flash Vaporization Megabore Liner can be packed with silanized glass wool to minimize the amount of nonvolatile residues reaching the analytical column. In all cases, the Megabore column seals in the tapered region of the liner, ensuring a complete transfer of the sample from the inlet into the column. Liners are designed to fit J&W Megabore Conversion Kits only and are not direct replacements for Agilent part numbers. Liners Description Length (mm) Part Number (P/N) Price ($) Direct Injection Liner 1/4" Port Direct Injection Liner - HP G 30 Direct Injection Liner *Direct Injection Liner 1/4" Port A B C *Direct Injection Liner - HP G 30 Hot On-column Liner 1/4" Port Hot On-column Liner - HP G 20 Hot On-column Liner Silanized Flash Vaporization MegaboreLiner (This liner has no top restriction; it can be packed and cleaned easily.) Liner Ferrules for J&W Conversion Kits Description Part Number (P/N) Price ($) Graphite/vespel liner 6 mm (set of 10) Graphite Ferrule for 6 mm Mega/Split-Splitless Injection Port Liner, Double Taper (set of 10) * These liners are not precut to fit specific injectors. They must be cut to the proper length before use. 104 www. v priced-ap.p /21/01, 6:00 PM

106 Ferrules Agilent Technologies supplies graphite and graphite/vespel TM ferrules for your chromatographic needs. We package ferrules in wide-mouthed glass vials for easy removal and high product purity. Ferrules All ferrules come in packages of ten, except as noted. Graphite 15% Vespel/85% Graphite Description Column Size (I.D.) Part Number (P/N) Price ($) Part Number (P/N) Price ($) Varian, Perkin-Elmer, and Swagelok 1/16" Nuts and Fittings 0.4 mm I.D mm mm I.D mm mm I.D & 0.53 mm /8" Nuts and Fittings 0.4 mm I.D mm mm I.D mm mm I.D & 0.53 mm /4" Nuts and Fittings 1/4" (O.D.) Tubing Agilent 5880, 5890 (Long Nut) 0.4 mm I.D mm mm I.D mm mm I.D & 0.53 mm , 5890 (Short Nut), mm I.D mm mm I.D mm mm I.D & 0.53 mm MSD 85% Vespel/15% Graphite 0.4 mm I.D mm mm I.D & 0.53 mm You want it, we ve got it. Agilent continually strives to provide the best customer service available. This means providing you with high quality J&W brand columns that are available when you need them. We stock over 1100 different part number configurations so when you place an order you ll get the column you need. How can we do this? Virtually all of J&W s phases are synthesized in-house by experienced polymer chemists Our highly automated manufacturing process ensures absolute reproducibility Every J&W column is individually tested, and the results are packaged with each column And of course, a little bit of elf magic www. 105 v priced-ap.p /14/01, 2:11 PM

107 Flowmeters Agilent has a flowmeter for every taste... When choosing flowmeters and calibrators, you have two basic choices: Mass Flow Measurement Volumetric Flow Measurement Volumetric flowmeters measure virtually any gas and are easier to operate. Mass flowmeters are calibrated for specific gases and respond very quickly to changes in gas flow rates. Additionally, mass flow measurements are not affected by temperature and pressure fluctuations. Agilent offers both volumetric and mass flowmeters and three instruments that measure both! We manufacture the largest selection and most popular flowmeters for gas chromatography. In addition, we have developed flowmeters for industrial hygiene, research laboratories, and instrument verification operation. Which flowmeter should I use? Flow Rate Range Flowmeter Type What is Measured? Low High Benefit ADM Series Noncorrosive Gases 0.1 ml/min 1 L/min Ease of Use Veri-Flow 500 He, H 2, Air, N 2, Ar/CH 4 5 ml/min 500 ml/min Versatility MicroFlo 20 Helium / Hydrogen 0.1 ml/min 20 ml/min Column Flow Measurement Optiflow All Gases 0.1 ml/min 25 L/min Visible (Digital Bubble) Verification Liquid Flowmeter Liquids 1.0 ml/min 30 ml/min Liquid Flow The ADM Flowmeter All ADM flowmeters are battery powered and field portable. Agilent calibrates each instrument to 5-point, NIST-traceable standards to ensure the highest available accuracy. The ADM family measures flow volumetrically, so you don t have to make any adjustments when changing from one gas to another. ADM features include: Easiest to use GC flowmeter ADM1000 If you need rapid, real-time measurements of a gas stream, it s perfect for you. Flow rates from 0.1 to 1000 ml/min Split ratios compare the ratio from one gas measurement to another (i.e., injection port split ratios) ADM2000 In addition to the features of the ADM1000, the ADM2000 includes: Volume and mass flow measurements 9V battery and AC power adapter (120 or 220 VAC) ADM3000 If you measure flow rates for a variety of purposes, the ADM3000 offers you the most versatility. In addition to the features of the ADM1000, this model includes: Measurement of flow from positive and negative (vacuum) pressure sources 9V battery and AC power adapter (120 or 220 VAC) Accuracy +/-3% or +/-0.2 ml/min Accurate Calibration Traceable to NIST Primary Standards CE Mark Certified RS-232 Output ADM Flowmeters We recommend calibrating all flowmeters once a year. Please contact your local Agilent office or distributor for more information. Flow Rate(mL/min) Gases* Accuracy Power Part Number Price Description Low High Measured (%) Supply (P/N) ($) ADM all +/-3 9V Battery ADM all +/-3 Battery or 120 VAC U 649 ADM2000E all +/-3 Battery or 220 VAC E 649 ADM all +/-3 Battery or 120 VAC U 649 ADM3000E all +/-3 Battery or 220 VAC E 649 Carrying Case for ADM * Noncorrosive Gases Only 106 www. v priced-ap.p /21/01, 6:02 PM

108 Flowmeters Electronic Mass Flowmeters Veri-Flow 500 Need the ultimate in versatility? The Veri-Flow TM 500 packs an incredible amount of features into a highly accurate flowmeter. The solid-state gas sensor used in the Veri-Flow 500 measures mass flow rates of the five common GC laboratory gases from 5 to 500 ml/min. Above all, the Veri-Flow 500 has big features for a small, small price. 5 gases H 2, He, N 2, Air, Argon/Methane In-line Flow Rechargeable Battery Pack Two-way Data Communication through Serial Port +/- 3% Accuracy Calibrated to Primary Standard Electronic Mass Flowmeters MicroFlo 20 The Flowmeter for Carrier Gas Have you ever tried to accurately measure carrier gas flow rates with a flowmeter? Do you find measuring carrier gas linear velocity complicated and cumbersome? Measure capillary column flow rates accurately and easily with the MicroFlo TM 20. Measures He and H 2 from 0.05 to 20 ml/min Continuous Flow Readout 100-hour Operation on a 9V Battery +/- 3% Accuracy Flow Rate (ml/min) Gases Accuracy Power RS-232 Data Part Number Price Description Low High Measured (%) Supply Output (P/N) ($) Veri-Flow He, H 2, +/- 3 Rechargeable Yes HVF Ar/CH 4, Battery (110V N 2, Air Adapter) 220V Adapter HVF MicroFlo He, H 2 +/- 3 9V Battery None HMF www. 107 v priced-ap.p /21/01, 6:03 PM

109 Flowmeters Digital Bubble Flowmeters Agilent Digital Bubble Flowmeters have been making flow measurement easy for years. Today, they are still widely used to measure GC flows, calibrate air sampling pumps, and verify various gas flows. The Optiflow TM series offers the widest range of flow rates and allows you to visually ensure gas flows. These tried and true flowmeters measure volumetrically and, therefore, can measure any gas composition with the push of one button and the squeeze of a bulb. Optiflow features: Calibrated to NIST Primary Standard Wide Flow Range from 0.1 to 50 ml/min (Optiflow 420) up to 0.1 to 25 L/min (Optiflow 730) 9V Long-lifetime Battery Optiflow 420 The Optiflow 420 is the best bubble flowmeter for measuring low flow rates especially GC columns. Flow Range to 50 ml/min +/- 3% Accuracy Recalibration Available Optiflow 520 & Optiflow 570 Want a bubble flowmeter for measuring carrier, makeup, injection port, and detector gases? The Optiflow 520 and Optiflow 570 handle most of your GC needs. We also manufacture Optiflow 570 in a hard metal case, which makes it portable and field ready. Flow Range to 500 ml/min for the Optiflow 520 Flow Range to 700 ml/min for the Optiflow 570 Available in Hard Field Case (Optiflow 570, only) Split Ratio Measurement +/- 3% Accuracy Recalibration Available Optiflow 650 & Optiflow 730 If you calibrate air sampling equipment, verify mass flow controller settings, or monitor higher flow rates, the Optiflow flowmeters are simple and efficient. Flow Range to 5000 ml/min for the Optiflow 650 Flow Range to 25 L/min for the Optiflow 730 +/- 2% Accuracy Available in Hard Field Case (650 only) Measures both Positive and Negative (Vacuum) Flow Sources Recalibration Available Optiflow 1000 Optiflow 1000 measures liquids! Verify and set HPLC column flow rates with the Optiflow Flow Range - 1 to 30 ml/min (1000) +/- 2% Accuracy Recalibration Available Humonics Flowmeters Optiflow 520 Optiflow 650 Optiflow 1000 Flow Rate (ml/min) Gases* Accuracy Power Part Number Price Description Field Case Low High Measured (%) Supply (P/N) ($) Gas Flowmeters Optiflow 420 No all +/- 3 9V Battery HFM Optiflow 520 No all +/- 3 9V Battery HFM Optiflow 570 No all +/- 3 9V Battery HFM Field-Cal 570 Yes all +/- 3 9V Battery HFM-570-FC 525 Optiflow 650 No 5.0 5,000 all +/- 2 9V Battery HFM Field-Cal 650 Yes 5.0 5,000 all +/- 2 9V Battery HFM-650-FC 625 Optiflow 730 No ,000 all +/- 2 9V Battery HFM Liquid Flowmeters Optiflow /- 2 AC Adapter (110V) HFM Optiflow /- 2 AC Adapter (220V) HFM *Noncorrosive Gases Only 108 www. v priced-ap.p /21/01, 6:05 PM

110 Gas Purifiers Moisture Traps Split Vent Traps Split or splitless injection systems can vent up to 500 times the amount of sample being analyzed into the lab air. This high capacity unit traps a broad range of contaminants. Split Vent Trap Description Part Number (P/N) Price ($) Split Vent Trap w/ 3-pack of Cartridges RDT Replacement Cartridges (3-pack) RDT Split Vent Trap Refillable Moisture Traps Indicating Moisture Traps are designed to remove water, oil, and organics from gases employed in, but not limited to, gas chromatography. Refillable moisture traps are constructed from Lexan TM brand polycarbonate tubing. Lightweight, chemically resistant and of superior strength when compared to traps manufactured from acrylic, Agilent Indicating Moisture Traps have become the chromatographers, choice for water adsorption from GC gases. Available in three refillable sizes and three different packings Both inlet and outlet connectors are equipped with stainless steel frits, to prevent particulates from entering the gas stream Mixed packing bed makes improper installation impossible Easily refillable Tested to 150 psi (helium) Moisture Trap Composition Molecular Sieve 13X and Indicating Molecular Sieve 4Å The preferred adsorbent for GC gas drying. Blue indicating sieve turns buff at 20% relative humidity. Moisture Removal Maximum Effluent H 2 0 Size of Trap (cc) Removal Capacity (g) Concentration (ppb) Molecular Sieve 5Å and Indicating Drierite High moisture capacity and simultaneously removes hydrogen sulfide and oil. Indicating Drierite changes dramatically from bright blue to pink as the gas stream approaches 40% relative humidity. Moisture Removal Maximum Effluent H 2 0 Size of Trap (cc) Removal Capacity (g) Concentration (ppb) Silica Gel, Grade 40, and Indicating Silica Gel, Grade 42 Highest moisture capacity adsorbent. Adsorbs as much as 40% of its weight in water. High affinity for hydrocarbons. Blue indicating gel turns from a deep blue to pale pink at 40% relative humidity. Moisture Removal Maximum Effluent H 2 0 Size of Trap (cc) Removal Capacity (g) Concentration (ppm) www. 109 v priced_no pics-ap.p /22/01, 8:47 AM

111 Gas Purifiers Moisture Traps Molecular Sieve 13X and Indicating 4oÅ Description Size (cc) Fitting Part Number (P/N) Price ($) Refillable Moisture Trap 120 1/8" MT Refillable Moisture Trap 120 1/4" MT Refillable Moisture Trap 200 1/8" MT Refillable Moisture Trap 200 1/4" MT Refillable Moisture Trap 400 1/8" MT Refillable Moisture Trap MT120 Refillable Moisture Trap 400 1/4" MT Adsorbent Refill (1 pint) MSR-1 25 Mounting Clip for 120 & 200 cc MC Universal Mounting Clip for 400 cc UMC Molecular Sieve 5Å and Indicating Drierite Description Size (cc) Fitting Part Number (P/N) Price ($) Refillable Moisture Trap 120 1/8" MT120-2-D 60 Refillable Moisture Trap 120 1/4" MT120-4-D 60 Refillable Moisture Trap 200 1/8" MT200-2-D 79 Refillable Moisture Trap 200 1/4" MT200-4-D 79 Refillable Moisture Trap 400 1/8" MT400-2-D 94 Refillable Moisture Trap 400 1/4" MT400-4-D 94 Adsorbent Refill (1 pint) MSR-2 25 Mounting Clip for 120 & 200 cc MC Universal Mounting Clip for 400 cc UMC Silica Gel, Grade 40, and Indicating Silica Gel, Grade 42 Description Size (cc) Fitting Part Number (P/N) Price ($) Refillable Moisture Trap 120 1/8" MT120-2-S 60 Refillable Moisture Trap 120 1/4" MT120-4-S 60 Refillable Moisture Trap 200 1/8" MT200-2-S 79 Refillable Moisture Trap MT400 Refillable Moisture Trap 200 1/4" MT200-4-S 79 Refillable Moisture Trap 400 1/8" MT400-2-S 94 Refillable Moisture Trap 400 1/4" MT400-4-S 94 Adsorbent Refill (1 pint) SGR 25 Mounting Clip for 120 & 200 cc MC Universal Mounting Clip for 400 cc UMC www. v priced_no pics-ap.p /22/01, 8:50 AM

112 Gas Purifiers Glass Moisture Traps Glass Indicating Moisture Traps Ideal for GC/MS Systems, Electron Capture Detectors, and Electrolytic Conductivity Detectors Molecular Sieve 13X and a band of Indicating Molecular Sieve 4Å Available in 70 cc, 100 cc and new 250 cc sizes Heavy wall borosilicate glass tube no moisture diffusion Refillable Outlet Gas has less than 1% inlet concentration Polishing Zone Active Desiccant Mass Transfer Zone (MTZ) Gas at this point has less than 5% of inlet concentration Capacity and Efficiency The 100 cc unit will treat 10 standard A cylinders with up to 30 ppm water or 16.3 grams to less than 10 ppb. Molecular sieves differ from all other commercially available adsorbents, as they have an extremely high adsorption capacity for water and polar compounds even at relatively low concentrations. The color change takes place in the middle of the trap bed, giving ample warning. In addition to the inherent qualities of the sieve material, we treat the bed material under high vacuum and heat to ensure maximum scrubbing efficiency and capacity. Moisture Removal Maximum Effluent Size of Trap (cc) Removal Capacity (g) Concentration (ppb) Glass Indicating Moisture Traps Description Size (cc) Fitting Part Number (P/N) Price ($) Glass Indicating Moisture Trap 70 1/8" GMT-2GC-HP 80 Glass Indicating Moisture Trap 70 1/4" GMT-4GC-HP 80 Glass Indicating Moisture Trap 100 1/8" GMT-2-HP 90 Glass Indicating Moisture Trap 100 1/4" GMT-4-HP 90 Glass Indicating Moisture Trap 250 1/8" LGMT-2-HP 112 Glass Indicating Moisture Trap 250 1/4" LGMT-4-HP 112 Scrubber Zone Mounting Clip for 70 & 100 cc UMC Mounting Clip for 250 cc UMC Molecular Sieve Refill 250 GMSR 25 Desiccant is at equilibrium with inlet gas adsorbate concentration Inlet Gas Refillable Glass Moisture Trap www. 111 v priced-ap.p /15/01, 5:03 PM

113 Gas Purifiers O 2 /H 2 O Traps Oxygen/Moisture Traps The Oxygen/Moisture Trap is recommended by more gas chromatograph and capillary column manufacturers than any other brand. Since 1981, more than 300,000 units have been installed, with confidence, by chromatographers worldwide, to protect expensive columns and sensitive GC detectors from contaminants commonly found in laboratory gases. Consider the performance, versatility (can be used safely with hydrogen) and economical value of the Agilent Oxygen/Moisture Trap when comparing it to a heated/catalytic type unit. Compare for Yourself Optimized for Maximum Surface Area and Capacity Leak-free, One-piece Design (tested to 2000 psi) Bed Material Treated with Ultra-High Purity Helium Filter Design: Prevents Channeling, Promotes Efficient Scrubbing We recommend these traps for the following applications: Electron Capture Detectors Recommended by the major manufacturer of electron capture detectors, oxygen/water removal effectively prevents detector degradation from oxygen and water contamination. The all-metal housing of the O 2 /H 2 O Trap virtually eliminates contamination and resultant signal noise. GC/MS Here is the Proof! GC/MS Evaluation of the O 2 /H 2 O Trap TEST CRITERIA: Ability of the O 2 /H 2 O Trap to remove oxygen and moisture from helium. O 2 /H 2 O Trap Why use an Oxygen Trap? Traces of oxygen in your carrier gas system can destroy a GC column, especially polar phases. How does it work? The O 2 /H 2 O Trap contains a highly active, metal-containing, scrubbing material in an inert, aluminum body. This material removes oxygen from a gas stream to less than 15 ppb. Additionally, up to 2 grams of moisture can be removed by this trap without affecting the oxygen capacity. Application: The O 2 /H 2 O Trap is ideal for use with argon-methane mixtures. EXPERIMENTAL CONDITIONS: A. High Purity Helium B scans of mass spectral data were acquired. Helium admitted to MS during scans Ten scans were summed and scans were used to correct for inherent instrument background. C. Trap was installed and experiment was repeated. D. After additional use, the experiment was repeated. RESULTS: The data shows about a twentyfold reduction in the m/z 18 ratio (water) and about a fortyfold reduction in the m/z 32 ratio (oxygen). m/z RATIO* 18/28 32/28 32/40 Without O 2 /H 2 O Trap With O 2 /H 2 O Trap (installed between the gas cylinder and the GC) * Contribution of inherent instrument background removed. Analysis by: California Analytical Laboratories, Inc., Sacramento, California. In addition to inert gases (nitrogen, helium, argon and krypton), the O 2 /H 2 O Trap treats streams of hydrogen, alkanes, alkenes, aliphatic hydrocarbon gases, low boiling aromatics, carbon dioxide and carbon monoxide. Oxygen/Moisture Traps Description Size (cc) Tube Fitting Part Number (P/N) Price ($) Agilent O 2 /H 2 O Trap 300 1/8" OT Agilent O 2 /H 2 O Trap 300 1/4" OT Mounting Clip MC www. v priced_no pics-ap.p /22/01, 8:50 AM

114 Gas Purifiers Oxygen Traps Indicating Oxygen Traps Not Just an Indicator, but an Indicating Trap Reduces oxygen to less than 1 ppb Environmentally safe Regeneration service available Available in two sizes, standard and large-three times the capacity This trap actually removes the oxygen rather than converting it to another form of contamination. Oxygen and a wide range of oxides react with the activated bed material to form a manganese oxide. This reaction results in a progressive and dramatic color change, from light green, in the adsorbent s active state, to deep brown as oxygen saturation occurs. The Indicating Oxygen Trap is designed to be used in conjunction with any of our high capacity, non-indicating oxygen traps. When properly installed downstream from the nonindicating trap, the Indicating Oxygen Trap provides a visual indication of oxygen breakthrough BEFORE contamination reaches sensitive system components. Unlike adsorbent materials utilized in competitive products, our indicating media can be exposed to air and water in virtually any quantity without resulting in an exothermic reaction. The spent product is nontoxic, nonhazardous, nonflammable, and nonreactive. The high impact Lexan shield prevents lab catastrophes if the trap is exposed to pressure beyond its stated pressure limit. Safe for landfill refuse. Oxygen Removal Type of Trap Removal Capacity (mg) Performance (ppb) OT3 series IOT series LIOT series OT3 + IOT GC GC RBC series RQC series BOT series 3, Moisture Removal Type of Trap Removal Capacity (g) Performance MT120 series ppb MT120-D series ppb MT120-S series ppm MT200 series ppb MT200-D series ppb MT200-S series ppm MT400 series ppb MT400-D series ppb MT400-S series ppm GMT series ppb GMT-GC series ppb GC ppb GC-2-I ppb BMT series ppb Performance numbers are the lowest levels observed using N 1 L/min. Indicating Oxygen Traps Description Size (cc) Fitting Part Number (P/N) Price ($) Indicating O 2 Trap (IOT) 30 1/8" IOT-2-HP 85 Indicating O 2 Trap 30 1/4" IOT-4-HP 85 Large Indicating O 2 Trap (LIOT) 150 1/8" LIOT Large Indicating O 2 Trap 150 1/4" LIOT Mounting Clip for IOT UMC Mounting Clip for LIOT UMC Indicating Oxygen Trap www. 113 v priced_no pics-ap.p /22/01, 8:51 AM

115 Gas Purifiers Carbon Dioxide Traps Glass CO 2 Traps The Carbon Dioxide Trap removes CO 2 from gases in applications such as carbon-hydrogen determinations, especially in quantitative organic microanalysis and in the analysis of respiratory gases. The trap body is constructed of borosilicate glass. The fittings are nickel plated and have sintered stainless steel frits. This highly adsorbent material has excellent indicating properties. Typically, this material adsorbs 20-30% of its weight in carbon dioxide before it must be replaced. The greenish-brown trapping material turns white due to the formation of sodium carbonate. Install a moisture trap downstream to adsorb the water evolved from the CO 2 trap. CO 2 Traps Description Size (cc) Fitting Part Number (P/N) Price ($) Carbon Dioxide Trap 100 1/8" COT Carbon Dioxide Trap 100 1/4" COT Mounting Clip for 100 cc UMC Carbon Dioxide Trap 250 1/8" LCOT Carbon Dioxide Trap 250 1/4" LCOT Mounting Clip for 250 cc UMC CO 2 Adsorbent (500 g) Refill* COT-R 80 *When refilling the trap, exercise extreme caution The packing material is extremely caustic Use caution. Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions need. Just as we ve done for 25 years. Phone: (USA & Canada) Fax: (USA & Canada) Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Angelica Reese Technical Support Engineer 114 www. v priced-ap.p /14/01, 2:40 PM

116 Gas Purifiers Hydrocarbon Traps Hydrocarbon Traps While oxygen contamination is the most serious problem when considering the longevity and performance of a capillary column, hydrocarbon contamination is one of the most pervasive and damaging problems for sensitive detectors. FIDs, ECDs and Hall electrolytic conductivity detectors are especially vulnerable. With the Hall detector, sulfur compounds can combine with the heated hydrogen to form hydrogen sulfide, a compound that will poison a nickel reactor tube. This results in varying degrees of lower sensitivity or, ultimately, NO sensitivity at all! With ECDs, the problem is one of baseline noise, lower sensitivity, and eventual detector contamination. Hydrocarbon contaminants are frequently reduced proportionately less than oxygen and water as the purity level of a gas is increased. The sources of these impurities are common to most gas suppliers. Cylinders, for example, are filled on manifolds which are both susceptible to mechanical wear and cross contamination from one contaminated cylinder to another. Gases can also demonstrate some increase in contamination in tanks filled as industrial scrubbers that approach saturation. The metal walls of the cylinders themselves can release organic volatiles into an otherwise purified gas stream. Finally, outgassed organic contaminants of elastomeric materials from regulators and flow controllers will, over time, add unwanted organic contamination. Hydrocarbon Traps will reduce hydrocarbons in GC gases to levels undetectable by most conventional methods. Refillable Hydrocarbon Traps Remove organics from carrier gases, air and hydrogen High capacity 200 cc of filtering media Impregnated carbon filter media Refillable Capillary Grade Hydrocarbon Traps Extremely high surface area, coconut shell-based, activated carbon 100 cc of filtering media Purged with ultra-high purity helium Refillable Hydro-Moisture Traps Replace most mixed bed traps supplied by GC manufacturers Please call our Technical Support department at if you would like assistance in determining the efficiency of our hydrocarbon traps with your gas streams. Refillable Hydrocarbon Trap Ability of Activated Carbon to Remove Substances from Gases Compound Efficiency acetone excellent hypochlorous acid excellent alcohol excellent amines very good inorganic acids none ammonia poor iodine excellent amyl acetate excellent isopropyl acetate excellent amyl alcohol excellent isopropyl alcohol excellent benzene excellent ketones excellent butyl acetate excellent butyl alcohol excellent lactic acids excellent by-products-organic very good lysol excellent calcium hypochlorite excellent mercaptans excellent carbon dioxide none methyl acetate excellent chlorobenzene excellent methyl alcohol excellent chlorine excellent methyl bromide excellent chlorophenol excellent methyl chloride excellent chlorophyll excellent methyl ethyl ketone excellent cresol excellent Hydrocarbon Traps Compound Efficiency dissolved oils excellent nitrobenzenes excellent nitrotoluene excellent ethyl acetate excellent ethyl alcohol excellent organic acids excellent ethyl chloride excellent oxalic acid excellent ethyl ether excellent ozone excellent fluoride poor phenol excellent formaldehyde poor potassium permanganate excellent propyl acetate excellent glycol excellent propyl alcohol excellent propyl chloride excellent hydrogen bromide satisfactory hydrogen chloride poor sodium hypochlorite excellent hydrogen fluoride none solvents excellent hydrogen iodide satisfactory sulfuric acid satisfactory hydrogen selenide satisfactory hydrogen sulfide satisfactory toluene excellent trichloroethylene excellent xylene excellent Description Size (cc) Fitting Part Number (P/N) Price ($) Refillable Hydrocarbon Trap 200 1/8" HT Refillable Hydrocarbon Trap 200 1/4" HT Big Hydrocarbon Trap 750 1/8" BHT Big Hydrocarbon Trap 750 1/4" BHT Big Hydrocarbon Trap 750 1/2" BHT Refillable Hydro-Moisture Trap 200 1/8" HMT Refillable Hydro-Moisture Trap 200 1/4" HMT Capillary Grade Hydrocarbon Trap 100 1/8" HT Capillary Grade Hydrocarbon Trap 100 1/4" HT Mounting Clip for HT3 and HT200 Series MC Adsorbent Refill (1 pint) for 2 recharges (hydrocarbon only) ACR 25 Adsorbent Refill (1 pint) for 2 recharges (hydrocarbon/moisture) HCRMS 25 Adsorbent Refill for a Big Hydrocarbon Trap (2 refills) BACR 50 Big Mounting Clip (use 2 per trap) UMC www. 115 v priced_no pics-ap.p /22/01, 8:51 AM

117 Gas Purifiers High Pressure Traps High Pressure Stainless Steel Traps These reusable traps are designed for high pressure applications where plastic bodied purifiers are not suitable. Constructed from seamless 316 stainless steel, these one-piece housings are DOT rated at 4000 psig and tested up to 12,000 psig without measurable distortion. They can even be installed on the high pressure side of your GC system (commonly, traps can only be installed on the low pressure, instrument side). Each fitting is equipped with a 40 micron sintered stainless steel frit for particulate control. Agilent conditions all purifiers prior to packing, then purges them with ultra-high purity helium before shipping to remove moisture. High Pressure Stainless Steel Trap High Pressure Moisture Trap Packed with molecular sieve 13X for simultaneous removal of water and trace level CO 2. We recommend this unit for all GC applications to eliminate water interference of detectors. High Pressure Hydrocarbon Trap Packed with a coconut shell-based, activated carbon, the High Pressure Hydrocarbon Trap removes a wide variety of hydrocarbons, esters, ethers, alcohols, ketones, aromatics, and chlorinated hydrocarbons. Ideal for use with hydrogen and air for FIDs. High Pressure Oxygen/Moisture Trap Removes oxygen to less than 15 ppb. A must for any GC system. We use the same oxygen removal media as we use in our Oxygen/Moisture Traps (supplied to the majority of GC manufacturers). High Pressure Traps (up to 4000 psig) Description Size (cc) Fitting Part Number (P/N) Price ($) High Pressure Moisture Trap 200 1/8" MT-2-SS 165 High Pressure Moisture Trap 200 1/4" MT-4-SS 165 Moisture Trap Refill Kit (1 pt.) MSR-1X 20 High Pressure Hydrocarbon Trap 200 1/8" HT-2-SS 165 High Pressure Hydrocarbon Trap 200 1/4" HT-4-SS 165 Hydrocarbon Trap Refill Kit (1 pt.) ACR 25 Carbon Dioxide Trap Refill Kit (500 g)* COT-R 80 High Pressure Oxygen Trap 200 1/8" OT-2-SS 185 High Pressure Oxygen Trap 200 1/4" OT-4-SS 185 Mounting Clip MC * The packing material is extremely caustic use caution. What do J&W s tighter performance criteria do for you? The integrity of your analytical results are important. Don t be fooled by cheap imitations!!!! J&W s legendary commitment to product reliabilty and column to column repeatability has raised the level of quality chromatographers around the world demand. This world class performance is acheived by controlling all aspects of the manufacturing process from beginning to end. Feature Advantage Benefit Narrow Retention Highest level of column-to-column Confidence in analytical results Index & Retention reproducibility Factor Window Minimal method adjustment when changing columns Specify J&W chemistry for intra-company methodologies Improved sample throughput Reduced downtime Confidence in method transfer and intra-company results Increased Plates Highest level of resolving power Accurate quantification per Meter Improved sample throughput Potentially shorter run times Lowest bleed Increased analytical Reduced detection limits sensitivity for all detectors Smaller samples Reliable compound identification Fast baseline stabilization Faster column exchange Excellent thermal stability Minimized conditioning time Increased sample throughput Increased column lifetime Reduced downtime Increased sample throughput 116 www. v priced_no pics-ap.p /22/01, 8:52 AM

118 Gas Purifiers Big Traps Now you can have Jumbo Size 750 cc gas purifiers! The same dependable oxygen, moisture, and hydrocarbon removing traps that have made us the leader in GC gas filtration are now available in a mega trap volume. Ideal for bulk purification applications, or where several instruments are plumbed from a single gas source, these traps can provide a low cost alternative to higher priced, Chromatography Grade gases. Big Trap If you re one who thinks the bigger, the better, then these traps are for you! Big Oxygen Trap Capacity: 3 liters of O 2 or 3,200 mg Pressure: Up to 250 psig Efficiency: Reduction of O 2 to less than 1 ppb Big Moisture Trap Capacity: 130 grams of H 2 O Pressure: Up to 250 psig Efficiency: Reduction of H 2 O to less than 5 ppb Big Hydrocarbon Trap Capacity: 80 grams of medium to heavy molecular weight hydrocarbons Pressure: Up to 250 psig Efficiency: Reduction of C 4 hydrocarbons to less than 15 ppb NEW Big Hydrocarbon Trap Capacity: 750 cc high capacity, high flow unit Pressure: Up to 250psig Efficiency: Reduction of hydrocarbon concentration in gases to low ppb levels Trap Mounting Panel 17 x 24 Five rows for mounting traps Designed for UMC-1, UMC-2, UMC-3, UMC-4 and UMC-5 mounting clips The oxygen trap has the capacity to remove 3 liters of oxygen. The moisture trap will remove a minimum of 130 grams of water to a residual H 2 O level of less than 5 ppb. And, the hydrocarbon trap has the capacity to adsorb 80 grams of medium to heavy molecular weight hydrocarbons, using a high surface area, coconut shell-based media. Each trap is constructed from a single length heavy walled aluminum tube. This design eliminates the possibility of leaks due to faulty welds and promotes low dead volume bed packings and consistent flow during operation. All Big Traps are available with a choice of 1/8, 1/4 or 1/2 Swagelok TM brand tube fittings. All fittings are equipped with 40 micron sintered stainless steel frits to prevent particulates from becoming entrained downstream in back pressure regulators and flow controllers. All units are leak checked and purged with helium prior to shipping. An inexpensive refill kit is available for the moisture and hydrocarbon models. Big Traps Description Size (cc) Fitting Part Number (P/N) Price ($) Big Oxygen Trap 750 1/8" BOT Big Oxygen Trap 750 1/4" BOT Big Oxygen Trap 750 1/2" BOT Big Moisture Trap 750 1/8" BMT Big Moisture Trap 750 1/4" BMT Big Moisture Trap 750 1/2" BMT Refill for Big Moisture Trap (enough for 2 refills) BMSR-1 40 Big Hydrocarbon Trap 750 1/8" BHT Big Hydrocarbon Trap 750 1/4" BHT Big Hydrocarbon Trap 750 1/2" BHT Refill for Big Hydrocarbon Trap (enough for 2 refills) BACR 50 Big Mounting Clip (use 2 per trap) UMC Trap Mounting Panel Description Part Number (P/N) Price ($) Trap Mounting Panel UMP www. 117 v priced-ap.p /14/01, 2:41 PM

119 U Gas Purifiers Point of Operation Panels Quick Change (QC) This system is based on our highly successful cartridge model trap first developed in The system consists of 2-, 3- or 4-head configurations. Each head is equipped with inlet and outlet check-valve fittings (Figure 1), designed to minimize contaminant introduction during cartridge replacement. Filter cartridges are of all metal or glass construction, eliminating infusion and resultant signal noise associated with filters constructed from plastics. Cartridges are quickly installed via a simple knurled retaining nut. No wrenches are needed! As many as four cartridges can be replaced in a matter of seconds, and because there is low dead volume, a minimal amount of gas system purge is required after installation. Oxygen, water, hydrocarbons and other organics are reduced to low part per billion levels. Available with 1 / 8 " or 1 / 4 " connections, panels can be wall mounted or used free standing. Quick Change Plus (QC+) QC+ is the latest development in the evolution of our cartridge type Point of Operation Panels. Like the original panel, purifier cartridges can be quickly changed (that s the QC). Only now, cartridges are removed from the panel without interruption of gas flow to the system (that s the +), drastically minimizing costly instrument downtime (and that s a real +). Each QC+ filter head is equipped with an internal flow valve. This valve serves two functions. In the down position, gas is passed through adsorbent filter cartridges for purification (Figure 2a). In the up position, the gas is diverted through the filter head. With the valve up (Figure 2b), the filter cartridge can then be easily replaced with a fresh cartridge. But, because the gas continues to flow through the by-pass valve, the need to shut down instruments is eliminated while the filter cartridges are removed. QC+ is the only GC purification system with this revolutionary flow through/bypass feature. The QC+ system has a unique D-shaped cartridge top that is selfaligning into the matching slotted filter head, assuring trouble-free installations. As many as four cartridges can be replaced in a matter of seconds without exposing downstream plumbing to atmospheric contaminants. QC+ systems are available in singlehead kits or 2-, 3- and 4- head panel configurations, available exclusively with 1 / 8 " Swagelok fittings. 2a Outlet Replacement Cartridges High Capacity Oxygen The GC-1 oxygen cartridge is an all metal assembly that contains a highly active metal catalyst, supported on an alumina silicate substrate. Oxygen removal efficiency to less than 15 ppb. P/N: GC-1; Capacity: 396 mg High Capacity Moisture The GC-2 moisture cartridge is an all metal assembly that contains 13X molecular sieve, mesh sphere size. Water removal efficiency to less than 9 ppb. P/N: GC-2; Capacity: 15 g Indicating Moisture The GC-2-I indicating moisture cartridge is a glass assembly that contains a combination of 5Å molecular sieve and indicating Drierite. This cartridge provides a visual indicator of adsorbent saturation. Water removal efficiency to less than 9 ppb. P/N: GC-2-I; Capacity: 7 g Hydrocarbon The GC-3 hydrocarbon cartridge is an all metal assembly that contains a high surface area and coconut shell-based, activated carbon medium. Efficiencies to low ppb levels for C 2 and heavier compounds. Hydrocarbon removal efficiency to less than 30 ppb. P/N: GC-3; Capacity: 8 g Indicating Oxygen The GC-4 indicating oxygen cartridge is constructed from glass. It contains a manganese oxide adsorbent that experiences a dramatic and progressive color change during adsorption. Oxygen removal efficiency to less than 2 ppb. P/N: GC-4; Capacity: 40 mg 2b Outlet Figure 1. The checkvalve on the original QC stops flow during cartridge changes. Inlet Close valve to change cartridge Inlet Open valve during normal use Figures 2a & b. The plus (2a) features a closed gas path. When placed in the down position (2b), the plus valve shifts gas through the cartridge. 118 www. v priced-ap.p /28/01, 2:02 PM

120 Gas Purifiers Point of Operation Panels 2-, 3- and 4-Head Panels 2-Head GC Carrier Gas Quick Change System RBC-2, RBC- 4 or QC+ System RBC-P Two-head carrier gas purification system is strongly recommended when employing columns with bonded phases or any capillary column. Consists of high capacity oxygen/moisture adsorber and indicating trap that signals breakthrough of high capacity, nonindicating cartridge. Flow at Pressure: 13 LPM at 50 psi Helium 2-Head Detector Gas Supply for FID Quick Change System RBC-2-D, RBC-4-D or QC+ System RBC-D-P Two-head air/hydrogen system is strongly recommended for both hydrogen and air supply to Flame Ionization Detectors (FIDs). Includes high capacity moisture and hydrocarbon cartridges. Flow at Pressure: 14 LPM at 50 psi Helium 3-Head GCs with Selective Detectors and Carrier Gas Quick Change System RTC-2, RTC- 4 or QC+ System RTC-P Three-head cartridge system with high capacity oxygen, moisture, and hydrocarbon cartridges. The perfect compliment to any system that employs nitrogen, helium, or argon/methane mixtures. Flow at Pressure: 7 LPM at 50 psi Helium Original Quick Change Point of Operation Panels Description (cartridges) Fitting Part Number (P/N) Price ($) 1-Head High Capacity Oxygen 1/8" R2D High Capacity Oxygen 1/4" R2D Indicating Oxygen 1/8" R2D2-2-I 170 Indicating Oxygen 1/4" R2D2-4-I Head High Capacity Oxygen, Ind. Oxygen 1/8" RBC High Capacity Oxygen, Ind. Oxygen 1/4" RBC Moisture, Hydrocarbon 1/8" RBC-2-D 290 Moisture, Hydrocarbon 1/4" RBC-4-D Head Oxygen, Moisture, Hydrocarbon 1/8" RTC Oxygen, Moisture, Hydrocarbon 1/4" RTC Head O 2, Indicating O 2, HC, H 2 O 1/8" RQC O 2, Indicating O 2, HC, H 2 O 1/4" RQC Quick Change Plus (QC+) Point of Operation Panels Description (cartridges) Fitting Part Number (P/N) Price ($) 1-Head High Capacity Oxygen 1/8" R2D2-P 250 Indicating Oxygen 1/8" R2D2-I-P Head High Capacity Oxygen, Ind. Oxygen 1/8" RBC-P 590 Moisture, Hydrocarbon 1/8" RBC-D-P Head Oxygen, Moisture, Hydrocarbon 1/8" RTC-P 775 Moisture, Oxygen, Ind. Oxygen 1/8" RTC-I-P Head O 2, Indicating O 2, HC, H 2 O 1/8" RQC-P 1,050 3-Head Electron Capture and ELCD Detectors Quick Change System RTC-2-I, RTC-4-I or QC+ System RTC-I-P Three-head cartridge panel that includes high capacity oxygen, moisture, and indicating oxygen cartridges. Flow at Pressure: 7 LPM at 50 psi Helium Replacement Cartridges Description Part Number (P/N) Price ($) High Capacity Oxygen GC-1 55 High Capacity Moisture GC-2 55 Indicating Moisture GC-2-I 70 Hydrocarbon GC-3 55 Indicating Oxygen GC Head GC/MS and any Critical GC Application Quick Change System RQC-2, RQC-4 or QC+ System RQC-P Consists of high capacity oxygen, moisture, and hydrocarbon removing filters, plus an indicating oxygen cartridge to signal oxygen breakthrough of upstream unit. The ultimate in gas purification for any GC application. Flow at Pressure: 6 LPM at 50 psi Helium www. 119 v priced-ap.p /21/01, 6:32 PM

121 Gas Purifiers MegaSorp MegaSorp A Superior Helium Gas Purifier MegaSorp utilizes a layered, multi-adsorbent bed packing of the most effective, highest capacity adsorbent materials available today for the removal of oxygen, moisture, hydrocarbons, carbon dioxide and carbon monoxide from helium gas streams. The volume of the various adsorbent materials in the MegaSorp purifier was arrived at through rigorous testing and evaluation in order to assure that breakthrough of the five major contaminant groups occurs as simultaneously as possible as each material achieves complete saturation. One MegaSorp unit will easily purify the contents of thirteen K size cylinders of % purity helium to a cumulative level of 100 parts per billion of O 2, H 2 O, CO 2, CO and hydrocarbons at a flow rate of up to 8 liters/minute. All tube fittings are Swagelok brand stainless steel, fitted with 40 micron stainless steel frits for particulate control. Table 1 HIGH PURITY Helium % 0 2 < 5 ppm THC* < 1 ppm H 2 O < 5 ppm *Analysis limited to three contaminant groups Table 2 RESEARCH GRADE Helium % Impurities < 1.0 ppm N 2 < 0.5 ppm O 2 < 0.5 ppm THC* < 0.1 ppm CO+CO 2 < 0.1 ppm H 2 O < 0.2 ppm H 2 < 0.2 ppm Ar < 0.1 ppm Ne < 0.5 ppm *Total Hydrocarbons MegaSorp is more economical than other purifiers. MegaSorp costs about cents per cubic foot of gas treated, compared to cents per cubic foot with other purifiers. MegaSorp is ideal for any GC or GC/MS application where helium is employed and assurance of purity is essential. Combining the contents of three individual in-line traps into a single unit reduces the number of potential leaks and the possibility of aspirating contaminants into the gas stream. A single purifier also simplifies installation and replacement. MegaSorp has nearly three times the capacity of the competitor s purifier MegaSorp Gas Purifier Description Fittings Part Number (P/N) Price ($) MegaSorp 1/8 RMSH MegaSorp 1/4 RMSH Universal Mounting Clip (2 ea.) UMC MegaSorp will purify thirteen K size cylinders of % gas vs. competitor s five 120 www. v priced-ap.p /14/01, 5:02 PM

122 Gas Handling Dual Stage Pressure Regulator Agilent s Dual Stage Pressure Regulator provides steady gas pressures. The regulator is constructed with a 316 L stainless steel diaphragm to minimize contamination and promote regulator lifetime. The regulator body is made from nickel plated brass. Dual Stage regulators are provided with fittings to attach the regulator to a gas cylinder. The inlet pressure gauge reads up to 4000 psi, while the outlet pressure gauge reads up to 200 psi. Pressure Regulators Description Part Number (P/N) Price ($) Dual Stage Regulator (Hydrogen) Dual Stage Regulator (Ar, He, N 2 ) Dual Stage Regulator (Air) Spectra-Link is No Ordinary Flexible Tubing! Stainless Steel: no outgassing or permeation through polymeric materials Quick Connection: prevents air from entering gas lines during tank changeover Tested: each system has leak rates lower than 1x10-5 cc/sec Spectra-Link Description Part Number (P/N) Price ($) Spectra-Link with 1/8 fittings and 36 SS tubing SL Spectra-Link with 1/4 fittings and 36 SS tubing SL Spectra-Link Tubing Connecting System on a two-stage regulator www. 121 v priced-ap.p /21/01, 7:04 PM

123 Liners We offer a variety of liners to meet your needs. Split/Splitless and Megabore liners for manufacturers inlets are not designed for use in J&W inlet conversion kits. FocusLiner TM Improve reproducibility, improve results. The FocusLiner traps a glass wool plug in the ideal position in the injection port liner. At the point of injection, the glass wool provides extra surface area for vaporization, traps nonvolatile sample residue, and wipes any residual sample from the sample needle reproducibility is the result. Chambered The Chambered Split/Splitless features the unique T-Top design. This liner minimizes sample interaction with the injection port body through a restriction at the top and bottom of the liner. The T-Top design allows the analyst to disassemble the liner for easy packing with glass wool and removal of sample residue. Liners Flow Inverter TM /Cup Splitter These liners provide maximum flow path disruption and injection port residence time during split injections. As a result, your sample efficiently vaporizes and reduces injection discrimination. Cup Splitters are ideal for high molecular weight analytes when analyzed by split injection. Straight Straight Liners provide the ultimate flexibility for injections. You can easily pack these liners with glass wool. Also, Straight Liners can be easily cleaned and regenerated. Straight Liners can be used for both split and splitless injections. Gooseneck Single and Double Goosenecks, or restrictions at the top or bottom of the injection port, minimize sample interaction with the injection port body. The top restriction minimizes backflash, sample loss occurring when the volatilized sample is larger than the injection port volume. The bottom restriction minimizes interaction with the base of the injector, which makes this inlet liner ideal for trace level analyses. Manufacturer I.D. Wool 1/Pack 5/Pack Description (mm) Packing Dimensions P/N Price ($) P/N Price ($) Agilent 6890 / 5890/ 5880/ 4890 Straight 2.0 N 6.3 x Straight 4.0 N 6.3 x Straight 4.0 Y 6.3 x Single Gooseneck 4.0 N 6.3 x Double Gooseneck 4.0 N 6.3 x Hot On-column 4.0 N 6.3 x H H Megaliner - 22 Gauge 4.0 N 6.3 x H22 45 Megaliner Directly inject your sample into a 0.45 or 0.53 mm I.D. column. Solvent tailing is significantly reduced from straight liners as the column seals to the base of the liner. With this seal, sample discrimination is reduced as virtually all of the sample transfers to the column. Megaliner - 26 Gauge 4.0 N 6.3 x H26 45 (T-top) Flow Inverter 4.0 N 6.3 x (T-top) Focus Liner 4.0 Y 6.3 x Focus Liner, tapered 4.0 Y 6.3 x www. v priced-ap.p /14/01, 5:33 PM

124 Septa We offer several septa for the varied needs of today s chromatographers. Our septa order guide should ease your decision-making process and assure you get the right product for your application. Agilent packages its septa in widemouthed glass jars. Our packaging maintains high product purity and ensures easy removal of septa from the packaging. Our septa are packaged in quantities of 50 or the General Purpose Septa at 24 per pack. High Temperture Red ER The low bleed septum The ER septum provides lower bleed and higher temperatures for the most demanding analyses. High temperature ER septa operate up to 400 C. Premium Septa Up to 350 punctures per septum Two words summarize these septa, Longest Lifetime. Premium Gold Septa are designed for high throughput laboratories. These septa minimize the number of septum changes an analyst must make. Premium Gold Septa operate up to 350 C. Ultimate Green Septa If you like the other s green septa, try these. We have improved these septa to offer minimal bleed, improved lifetime, and we ve found that these septa are very easy to remove from an injection port. Ultimate Green Septa operate up to 350 C. General Purpose Septa (GPS) For injection temperatures up to 250 C, GPS offer long injection life (up to 100 punctures) and ease of sample injection. Septa Hewlett Packard 4890/5890/6890, Perkin Elmer, Varian Size Part Number (P/N) Price ($) High Temperature Red ER 7/16" 11 mm Premium 7/16" 11 mm Ultimate Green 7/16" 11 mm General Purpose 7/16"11 mm (24 per pk) acces03 9/ v priced-ap.p /14/01, 5:58 PM

125 Standards Column Test Standards Compare your column s performance to the test chromatogram shipped with your J&W column. The column test standard contains components that test the column for resolution characteristics, efficiency, and inertness. The test mixes are supplied at a concentration of 250 ng/µl in 2 ml vials. Match the phase and column diameter in the chart below to find the test mix for your column. If you would like a gas test mixture for a PLOT column, please call or fax our Technical Support Department. Order Guide Column Test Standards Column Description Microbore Capillary Megabore (0.05 & 0.10 mm I.D.) (0.18 to 0.32 mm I.D.) (0.45 & 0.53 mm I.D.) Part Number (P/N) Price ($) Part Number (P/N) Price ($) Part Number (P/N) Price ($) CAM Carbowax 20M OV Cyclodex-B TM CycloSil-B DB TM -1ht DB DB-1ms DB DB-5ht DB-5ms DB DB-17ht DB DB-17ms DB DB DB-35ms DB DB DB DB DB DB DB DB DB DB-ALC DB-ALC DB-Dioxin TM DB-FFAP DB-HT Sim Dis DB-MTBE DB-Petro DB-TPH DB-VRX DB-WAX DB-WAXetr DB-XLB DX DX DX SE SE SE acces03 9/26 v priced-ap.p /14/01, 6:02 PM

126 Tools Column Installation Kit Column Installation Kit The Capillary Column Installation Kit contains: Diamond-tipped pencil 20X magnifier Ferrule removal tool Column Installation Kit Septum removal tool Refillable 2 oz. bottle of Snoop Description Part Number (P/N) Price ($) Kit with Diamond-tipped Cutting Pencil Column Rinse Kit This kit makes solvent rinsing easy! Solvent rinsing removes many contaminants that accumulate in columns. The contaminants are removed by passing solvents through the column. Only bonded and cross-linked phase columns should be rinsed, or permanent damage to the column will result. Column Rinse Kit Description Part Number (P/N) Price ($) Bonded Phase Column Rinse Kit Capillary Rinse Kit Replacement 1/16" Teflon Ferrule Cutting Tools: Pencils and Ceramic Wafers We offer two conveniently designed, pencil-shaped tools and a ceramic wafer that allow you to make clean and easy cuts in fused silica, glass and aluminumclad capillary columns. Cutting Tools Description Part Number (P/N) Price ($) Cutting Pencils Diamond-Tipped Column Cutting Pencil Sapphire Column Cutting Pencil Ceramic Wafer (4/pk) Small Ferrule Tools Ferrule Tools Remove ferrules stuck inside fittings. Screw the stainless steel Ferrule Tool into the ferrule and pull it out. Use the smaller Capillary Ferrule Tool for capillary column ferrules (0.32 mm I.D. column and smaller). The Megabore Ferrule Tool is used for Megabore ferrules. The Liner Tool can be used to remove polyimide and tubing from inside a Megabore liner. Ferrule Tools Description Part Number (P/N) Price ($) Capillary Ferrule Tool RFT Megabore Ferrule Tool RFT Liner Tool RFT www. 125 v priced-ap.p /22/01, 9:06 AM

127 Tools 20X Magnifier Examine column ends for proper cuts. Jagged, rough, or nonperpendicular cuts negatively affect chromatographic performance. Microprobes Ideal for Removing Septa & Ferrules Hardened stainless steel probes remove septum and ferrule pieces stuck in fittings. Five different styles give you all of the angles necessary for getting into those hard-to-reach places. Column Installation Tools: Magnifier, Mirror and Microprobes Mirror Inspect injection ports, the inside of fittings, and other hard-to-reach places. Tools Description Part Number (P/N) Price ($) Mirror X Magnifier Five Microprobes RMP Five Microprobes Snoop Leak test most pressurized gas systems. We recommend that leak detecting solutions be used with care where there is any possibility of the fluid being aspirated into gas flow streams supplying the column or detector. This squeezable bottle comes with a Snooper Tube extendable up to 12 inches (30 cm). Electronic leak detectors are preferred. In their absence, a volatile nonreactive solvent like isopropanol may be used. Use caution to avoid aspiration of materials such as Snoop into the column. Snoop Description Part Number (P/N) Price ($) Snoop, 8-ounce (236 ml) bottle Snoop, 1- gallon bottle Vial Crimper and Decapper These tools are used with vials and caps for HP/Agilent autosamplers. Vial Crimper and Decapper Description Part Number (P/N) Price ($) Manual Crimper for all 11 mm seals (HP ) Manual Decapper for all 11 mm seals Crimping Tool 126 www. v priced-ap.p /22/01, 9:07 AM

128 Tools Injection Port Cleaning Kit If changing the liner and trimming the column does not remove contamination from your injection port, you probably need to clean and flush your injection port. Three stainless steel brushes and a scraper are the ideal tools for removing sample residue and septum particles. The stainless steel brushes have diameters of 5 mm, ¼ and 3/8. Deluxe Injection Port Cleaning Kit FID Cleaning Kit This easy-to-use maintenance kit improves sensitivity, reduces noise and decreases detector spiking. Cleaning Kits Description Part Number (P/N) Price ($) FID Cleaning Kit FID Cleaning Kit Injection Port Cleaning Kit Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions you need. Just as we ve done for 25 years. Phone: Fax: Web: Angelica Reese Technical Support Engineer (USA & Canada) (916) (USA & Canada) If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. www. 127 v priced-ap.p /14/01, 11:33 PM

129 Tubing Fused Silica and Stainless Steel Deactivated Tubing Deactivated tubing can be used as retention gaps, guard columns or transfer lines. Our standard deactivation process is a phenyl methyl deactivation the preferred choice for most applications due to its inertness and robustness. Fused Silica, Deactivated I.D. (mm) O.D. (mm) Length (m) Part Number (P/N) Fused Silica, High Temp (400 C) Deactivated I.D. (mm) O.D. (mm) Length (m) Part Number (P/N) ProSteel Stainless Steel, Deactivated I.D. (mm) O.D. (mm) Length (m) Part Number (P/N) www. v unpriced-ap.p /14/01, 10:24 AM

130 Tubing Fused Silica Undeactivated Tubing Undeactivated tubing or bare fused silica is commonly used for capillary electrophoresis. It can also be used for transfer lines and other applications where inertness is not critical. Fused Silica, Undeactivated I.D. (mm) O.D. (mm) Length (m) Part Number (P/N) Price ($) Fused Silica, High Temp (400 C) Undeactivated I.D. (mm) O.D. (mm) Length (m) Part Number (P/N) Price ($) ProSteel Stainless Steel, Undeactivated I.D. (mm) O.D. (mm) Length (m) Part Number (P/N) Price ($) (1/16") www. 129 v priced-ap.p /14/01, 7:19 PM

131 Tubing Accessories Guard Column Replacement Parts Six guard column replacement parts are available. This includes 10 meter lengths of deactivated fused silica tubing and 5 universal glass unions. Polyimide Sealing Resin Permanently connect fused silica columns to glass unions, liners and guard columns. Polyimide Sealing Resin Description Part Number (P/N) Price ($) Polyimide Sealing Resin (5 grams) Guard Column Kits Description (mm I.D.) Part Number (P/N) Price ($) 0.25 replacement tubing (10 meter guard column) replacement union (5 pack) replacement tubing (10 meter guard column) replacement union (5 pack) replacement tubing (10 meter guard column) replacement union (5 pack) Outstanding Technical Support It takes great people to make great products. And Agilent s J&W Scientific columns are backed by the best in the business. Go ahead. Call us on it. Anytime. Don t take our word for it. Give us a call, free of charge. (We also respond equally well to fax or online.) No matter how you contact us though, we think you ll agree that Agilent s technical support staff still comes through with the knowledge and answers you want and the solutions you need. Phone: (USA & Canada) Fax: (916) (USA & Canada) Web: If you are outside of the U.S.A. please contact your local dealer or Agilent office. See page 138. Cameron George, Sr. Technical Support Engineer 130 www. v priced-ap.p /14/01, 11:34 PM

132 Unions Zero Dead Volume Press Fit Unions Universal press fit, zero dead volume connectors join fused silica tubing. Available in glass or fused silica. One size fits all tubing diameters. For best results, use polyimide sealing resin. Press Fit Unions Description Part Number (P/N) Price ($) Glass Union, universal 2-way, 5/pk Glass Union, universal 2-way, 25/pk Glass Union, universal 3-way Fused Silica Union, universal 2-way, 5/pk Fused Silica Union, universal 2-way, 25/pk Fused Silica Union, universal 3-way J&W Chemistry Set for Dogs J&W Chemistry Set for Dogs* Let s face it. Dogs, like humans, are social animals. What better tool to help your dog determine where his friends have been than the Chemistry Set for Dogs. Watch with pleasure as your favorite pooch investigates favorite doggie hot spots such as sidewalks, shrubbery, park benches, tires and, of course, fire hydrants. The J&W Chemistry Set contains a library of over 1000 exciting smells. With a knick knack, paddy whack, give your dog the J&W Chemistry Set for Dogs. J&W Chemistry Set Full range of canine-size GC columns Mini GC fitted with dewclaw controls Doggie lab coat and safety glasses Optional feline adapters Description Part Number (P/N) Price ($) J&W Chemistry Set for Dogs 900-dogchem 326 Optional Feline Adapters 900-catchem 32 * A little elf humor, folks. Please do not try to order this product or contact the dog shown in the photo. www. 131 v priced-ap.p /22/01, 9:08 AM

133 Solid Phase Extraction (SPE) The Accubond II Difference Precision Solid Phase Extraction Cartridges - Sample Cleanup and/or Concentration Accubond II Solid Phase Extraction (SPE) is a costeffective alternative to liquidliquid extraction that minimizes solvent usage and requires less time per sample. SPE can be used for preparation of samples for GC, LC, RIA, MS, NMR, UV/VIS, AA and more. Individually performance tested cartridges ensure maximum reproducibility. No reliance on immiscibility of solvents, thus a wide range of solvents can be used. Free from emulsion formation. Cleaner extracts result in longer column life; pre-washed packings, frits and cartridges. Rapid, multisample processing yields higher sample throughput and more laboratory time for data analysis. The Agilent Difference: Reproducibility Looking for an SPE cartridge that provides consistent quality and reproducibility? The search is over. We have conquered the problem of lot-to-lot reproducibility. Our proprietary bonding process ensures consistency. Quality Control Certificate of Performance The Definitive Testing Protocol for SPE Cartridge Performance Condition/ Load sample Performance We go beyond testing bulk material, by testing random cartridges within a manufacturing lot. This added testing ensures performance reproducibility. A Certificate of Performance is provided in each box showing the results of several key performance parameters. Complete Line of Phases We offer a full line of phases to suit your extraction requirements. Our wide variety of sorbent chemistries and cartridge geometries ensures an appropriate SPE cartridge for your applications. Most phases are available in 100 mg, 200 mg, 500 mg and 1000 mg sizes. Rigorous testing guarantees reproducible SPE cartridges. We have achieved the first cartridge test protocol that probes individual cartridge performance. A carefully selected set of probe compounds is passed through the SPE cartridge, simulating cartridge use. We have selected the compounds on their ability to interact with the phase. The volume of solvent used to elute the desired analyte is a primary concern when using an SPE cartridge. We provide peak area values for the probe analytes during quality control testing. These indirectly measure related factors that affect elution solvent volume: packing density and flow rate. The measurement of these parameters ensures a consistently packed cartridge for those critical extraction protocols. Wash step removes impurities and unretained compounds Elute with selective solvent Purified analyte cleanly separated and ready for analysis Undesired components Analyte of interest Matrix impurity, interference Typical Solid Phase Extraction Sequence 132 v priced-cx-ap.p /15/01, 12:56 AM

134 Solid Phase Extraction (SPE) EVIDEX II Cartridges EVIDEX II - Rapid Solid Phase Extraction of Drugs of Abuse from Urine Designed for NIDA* drug classes Step-by-step instructions Robust analytical methods One cartridge for all methods Accurate, reproducible results The benchmark of any test is accuracy and repeatability. EVIDEX IITM incorporates a specially designed phase and carefully developed methods to provide the confidence that true, accurate values are obtained. When using EVIDEX II methods, typical results will show relative standard deviations (RSDs) of less than 5% for all drugs tested (Figure 1). We have designed each EVIDEX II method to be tolerant of inevitable measurement errors. The volumes and concentrations of the reagents have been designed to allow for slight deviations from the stated method values. Consequently, minor errors do not adversely affect the results (Figure 2). EVIDEX II is designed to extract these drugs of abuse: PCP Cocaine Benzoylecgonine Marijuana (THC-COOH) Codeine Morphine Amphetamine Methamphetamine *National Institute on Drug Abuse Extraction Recovery Levels Using EVIDEX II Recovery levels: x ± %; n = 8 EVIDEX II Is Forgiving Level in ng/ml Amphetamine 76±4 74±2 96±3 Methamphetamine 85±1 71±3 98±3 Level in ng/ml Benzoylecgonine 98±8 93±4 97±3 Level in ng/ml PCP 90±3 91±2 88±3 Level in ng/ml Codeine 99±1 97±3 98±1 Morphine 96±2 96±3 93±4 Level in ng/ml THC-COOH 92±4 96±894±3 Figure 1. Recovery Table Standard 2.7 ml method 0.10 N HCI EVIDEX Order Guide 3.3 ml 0.10 N HCI 3 ml 0.09 N HCI 3 ml 0.11 N HCI 2.7 ml Elution solvent 3.3 ml Elution solvent Description Part No. (P/N) Price ($) EVIDEX II SPE cartridges, 400 mg, 6 ml (30-pk)** ** Includes instructions on use Figure 2. Effect of varying method conditions on benzoylecgonine recovery (standard method = 1.0) 133 v priced-cx-ap.p /15/01, 12:56 AM

135 Solid Phase Extraction (SPE) Accubond II Order Guide Accubond II Order Guide Description Sorbent Column No. Per Part No. Price Weight Size Box (P/N) ($) Reversed Phase (Nonpolar) ODS (C18) 100 mg 1 ml mg 3 ml mg 3 ml mg 6 ml mg 6 ml Octyl (C8) 100 mg 1 ml mg 3 ml mg 3 ml mg 6 ml mg 6 ml Methyl (C1) 100 mg 1 ml mg 3 ml mg 3 ml mg 6 ml mg 6 ml Phenyl 100 mg 1 ml mg 3 ml mg 3 ml mg 6 ml mg 6 ml Normal Phase (Polar) Silica 100 mg 1 ml mg 3 ml mg 3 ml mg 6 ml mg 6 ml Cyano (CN) 100 mg 1 ml mg 3 ml mg 3 ml mg 6 ml mg 6 ml Diol 100 mg 1 ml mg 3 ml mg 3 ml mg 6 ml mg 6 ml Amino (NH 2 ) 100 mg 1 ml mg 3 ml mg 3 ml mg 6 ml mg 6 ml Ion Exchange Strong Anion 100 mg 1 ml Exchange 200 mg 3 ml (SAX) 500 mg 3 ml mg 6 ml mg 6 ml Strong Cation100 mg 1 ml Exchange 200 mg 3 ml (SCX) 500 mg 3 ml mg 6 ml mg 6 ml v priced-cx-ap.p /15/01, 5:30 PM

136 Solid Phase Extraction (SPE) Accubond II Order Guide Cont. Accubond II Order Guide Description Sorbent Column No. Per Part No. Price Weight Size Box (P/N) ($) Non-silica Alumina A 100 mg 1 ml (acidic) 200 mg 3 ml mg 3 ml mg 6 ml ,000 mg 6 ml Alumina B 100 mg 1 ml (basic) 200 mg 3 ml mg 3 ml mg 6 ml ,000 mg 6 ml Alumina N 100 mg 1 ml (neutral) 200 mg 3 ml mg 3 ml mg 6 ml ,000 mg 6 ml Florisil 100 mg 1 ml mg 3 ml mg 3 ml mg 6 ml ,000 mg 6 ml Evidex Specialty Phase EVIDEX II 400 mg 6 ml (Drugs of Abuse) SPE Accessories Accubond II Bulk SPE Sorbents The same quality sorbents found in the Accubond Solid Phase Extraction cartridges are available in bulk for use in preparative applications. Particle size: 40 µm; pore size: 60 Å; 25 g bottles. Accubond II Bulk SPE Sorbents Order Guide Description Part No. Price (P/N) ($) ODS (C18) Octyl (C8) Methyl (C1) Phenyl Silica Diol Cyano (CN) Amino (NH 2 ) SAX SCX Alumina A Alumina N Florisil Sorbents Accubond II SPE Method Development Kits Contain 10 each of the indicated phases in 100 mg, 1 ml configuration. Accubond II SPE Method Development Kits Order Guide Description Part No. (P/N) Price ($) Kit A Reverse phase C18, C8, C1, CN, Phenyl Kit B Normal phase Si, NH 2, Diol, CN Kit C Ion exchange SAX, SCX, NH v priced-cx-ap.p /15/01, 5:57 PM

137 Solid Phase Extraction (SPE) SPE Accessories Vacuum Manifold and Accessories Ten- and 20-port vacuum manifolds; include vacuum gauges with coarse and fine control, safety release valve, adjustable height 16-mm rack (standard; 10- and 12-mm optional), stainless steel needle tips. Optional PTFE stopcocks for individual cartridge control. SPE Accessories Order Guide for 10 and 20 Port Manifolds Description Part No. (P/N) Price ($) SPE vacuum manifold 10-port Rack (Optional), 10 X 10-mm, adjustable height Rack (Optional), 10 X 12-mm, adjustable height SPE vacuum manifold 20-port Rack (Optional), 20 X 10-mm, adjustable height Rack (Optional), 20 X 12-mm, adjustable height Stopcock Valves, Pack of 10, PTFE* Stainless Steel Needles for Vacuum Manifolds, Pack of Needle Retainer for Vacuum Manifolds, Pack of Lid Gasket for 10 position manifold Lid Gasket for 20 position manifold * Need two packs for 20-position manifold SPE Accessories Order Guide for J&W Manifold Description Part No. (P/N) Price ($) Vacuum gauge, 1/8" NPT, 0-30" HG Port sealing plugs (12) Gasket Luer fittings (12) Teflon tubing guides (12) Vacuum Manifolds - 20 port (left) and 10 port (right, disassembled) SPE Cartridge Reservoirs and Stopcocks SPE Cartridge Reservoirs and Stopcocks Order Guide Description No. Per Box Part No. (P/N) Price ($) Empty Reservoirs, Luer fitting on bottom 3 ml ml ml ml Frits for Reservoirs* 3 ml* ml* Reservoirs with 2 X 20-µm frits** 3 ml ml * Two frits in each tube. ** Used with High Suspended Solids Samples. 136 v priced-cx-ap.p /15/01, 9:08 AM

138 General Index A Acrylate Impurities Agilent Offices Alcohols... 88,89 Amines Aroclors B Blood Alcohols Books C CAM Carbowax 20M Chemistry Set for Dogs Chiral Chromatograms Conversion Kits & Liners Cyclodex-B CycloSil-B Compound Index Column Configurator... 8 Custom Columns... 7 D DB DB-1ht DB-1ms DB DB-5ht DB-5ms DB DB DB-17ht DB-17ms DB DB DB-35ms DB DB DB DB-225ms DB DB DB DB DB DB-1701P DB DB-ALC1 & DB-ALC DB-Dioxin DB-EVDX DB-FFAP DB-HT Sim Dis DB-MTBE DB-Petro DB-ProSteel DB-TPH DB-VRX DB-WAX DB-WAXetr DB-XLB Drugs Dual Columns DX-1, DX-3, DX E EPA Explosives F FAMEs Ferrules Flowmeters Fragrance Oils Fragrances Freight Freons G Gas Handling Gas Purifiers - Moisture Traps Big Traps Carbon Dioxide Traps High Pressure Traps Hydrocarbon Traps MegaSorp O 2 /H 2 O Traps Oxygen Traps Point of Operation Panels Gasoline GS-Alumina GS-CarbonPLOT GS-GasPro GS-Q H Haloacetic Acid Methyl Esters High Temperature Simulated Distillation HP-PLOT Al KCI HP-PLOT Molesieve Hydrocarbons... 93,97-99 L Liners Low Bleed... 4 M MTBE O Order Information Organic Acids... 76,90,92 Organochlorine Pesticides Organophosphorous Pesticides OV P Part Number Index PCB Congeners Permanent Gases Phenoxy Acid Methyl Esters Phthalates Pricing Q QC Specs Quality Control Specifications S SE Series Semivolatiles Septa Solid Phase Extraction Solvent Retention Index Solvents... 85,92,93 SPE Standards Sugars... 76,77 Sulfur Compounds... 94,95 T Tools Traps Trademarks Triglycerides Tubing Accessories Tubing- Fused Silica U Unions- Zero Dead Volume V Volatiles ,62 W Warranties www. 139 v priced-ap.p /22/01, 1:31 PM

139 Compound Index Part No.... Page Part No.... Page Part No.... Page Part No.... Page Acenaphthene... 68,70 Acenaphthene-d Acenaphthylene... 69,70 Acetaldehyde Acetaminophen Acetic acid... 76,90,92 Acetone... 58,76,78,80,81,85,86,93 Acetonitrile Acetophenone Acetylene... 93,95,97-99 Acetylsalicylic acid Acrylamide Acrylonitrile Alachlor... 66,69 Aldrin ,69 Allyl butyrate... 78,80 Allyl chloride... 58,62 Ametryn Amino-2,6-dinitrotoluene Amino-4,6-dinitrotoluene Aminobiphenyl N-(2-Aminoethyl)piperazine Aminoethylethanolamine tert-amyl methyl ether (TAME) n-amyl salicylate... 78,80 Aniline... 70,91 Anthracene... 69,70 Arabinitol D-Arabinose Arachidic acid (eicosanoic acid) Arachidic acid methyl ester Arachidonic acid methyl ester Aspon Atrazine... 67,69 Azinphos-ethyl Azinphos-methyl Behenic acid methyl ester Benzaldehyde... 78,80 Benzene ,63,85,92,93,97,99 Benzeneethanol Benzidine Benzo[a]anthracene... 69,70 Benzo[a]pyrene... 69,70 Benzo[b]fluoranthene... 69,70 Benzo[g,h,i]perylene... 69,70 Benzo[k]fluoranthene... 69,70 Benzoic acid Benzonitrile Benzophenone... 78,80 Benzyl acetate Benzyl alcohol... 70,78,80,88,89 Benzylamine Benzyl benzoate... 68,78-81 Benzyl butyl phthalate Benzyl chloride... 62,63 N-Benzylmethylamine Benzyl salicylate trans-α-bergamotene Bertenex α-bhc ,69 β-bhc ,69 δ-bhc... 64,65,66,69 γ-bhc ,69 BHT β-bisabolene Bolstar Borneol ,83 Bornyl acetate... 78,79 Bromacil Bromazepam Bromo-2-nitrobenzene Bromoacetic acid Bromoaniline Bromobenzene ,62,63 2-Bromobiphenyl Bromochloroacetic acid Bromochlorodifluoromethane Bromochloromethane... 57,61,62 Bromodichloroacetic acid Bromodichloromethane... 58,61,62 4-Bromoflouorobenzene... 57,58 Bromoform... 58,61,62 Bromomethane ,62 2-Bromopentane Bromophenyl phenyl ether Bromopyridine Bromotrifluoromethane Bufotenine Butabarbital Butachlor ,2-Butadiene ,3-Butadiene Butane... 93,95 n-butane... 96,97,98 2,3-Butanediol ,3-Butanedione (diacetyl)... 78,80 1-Butanethiol n-butanol... 85,88-90 sec-butanol... 85,88,89 tert-butanol... 85,88,89 2-Butanone Buten-1-ol... 88,89 2-Buten-1-ol (Crotyl alcohol)... 88,89 1-Butene trans-2-butene n-butene trans-2-butene Butoxyethanol (Butyl cellosolve)... 88,89 bis(2-n-butoxyethyl) phthalate Butyl acetate Butyl acrylate Butylate n-butylbenzene ,62,63,92 sec-butylbenzene ,62,63,92 tert-butylbenzene ,62,63,92 Butyl benzyl phthalate... 68,70 1-Butyl mercaptan Di-n-butyl phthalate... 68,70 Butyl propionate Butyne Butyne Butyric acid... 76,90,92 Butyric acid methyl ester δ-cadinene Caffeine... 85,86 Camphene Camphor ,83 Capric acid methyl ester Caproic acid (hexanoic acid)... 76,90,92 Caproic acid methyl ester Caprylic acid methyl ester Captafol Captan Carbazepine Carbazole Carbon dioxide... 57,95,96 Carbon disulfide... 58,93,94 Carbon monoxide Carbon tertachloride... 57,58,61,62 Carbonyl sulfide... 94,95 Carbophenothion Carboxin Carene Carvonel... 78,79 β-caryophyllene Caryophyllene Celestocide Cetearyl decanoate... 78,80 Cetearyl octanoate... 78,80 α-chlordane ,69 γ-chlordane ,69 Chlorfenvinphos Chloroacetic acid Chloroacetonitrile Chloroaniline Chloroaniline Chloroaniline... 70,91 Chlorobenzene ,62,63,92 Chlorobenzene-d Chlorobenzilate... 66,69 4-Chlorobenzonitrile Chlorobiphenyl Chlorobutane Chlorodibromoacetic acid Chloro-1,1-difluoroethane Chlorodifluoromethane Chloroethane... 56,61,62 bis(2-chloroethoxy) methane bis(2-chloroethyl) ether Chloroethyl vinyl ether... 62,63 Chloroform... 57,61,62,85 1-Chloro-3-fluorobenzene... 58,62,63 bis(2-chloroisopropyl) ether... 62,63,70 Chloromethane... 56,61,62 4-Chloro-3-methylphenol Chloronaphthalene Chloronaphthalene Chloroneb... 66,69 4-Chloro-3-nitrobenzotrifluoride Chloro-3-nitrotrifluoride Chloropentafluoroethane Chlorophenol Chlorophenol Chlorophenyl phenyl ether Chloroprene... 62,63 Chloropropham Chloropropane Chloropropylate Chloro-1,1,1,2-tetrafluoroethane Chlorothalonil... 66,69 2-Chlorotoluene ,62,63,92 3-Chlorotoluene Chlorotoluene ,62,63,92 Chlorpyrifos Chlorpyrifos-methyl Chrysene... 69,70 Chrysene-d ,8-Cineole Cinnamyl cinnamate... 78,80 Cinnamyl phenyl acetate... 78,80 Citronellal... 78,82 Citronellol Citronellyl acetate Citronellyl formate Citronellyl propionate... 78,79 Citronellyl tiglate Cocaine Codeine α-copaene Coumaphos Crotoxyphos Cumene... 56,97 Cyanazine Cyanopyridine Cyanopyridine Cycloate Cyclohexane... 85,97 Cyclohexanol... 88,89 Cyclopentane Cyclopentanol... 88,89 Cyclopentene Cyclopropane... 95,98,99 p-cymene ,4-D Dacthal... 66,69 Dalapon ,4-DB ,4'-DDD p,p'-ddd... 64,66,69 4,4'-DDE p,p'-dde... 64,66,69 4,4'-DDT p,p'-ddt... 64,66,69 Decachlorobiphenyl Decanal Decanoic acid Decanol... 88,89 Decanol n-decylamine DEF Demeton-O Demeton-S Desipramine Desmethyldiazepam Diallate A Diallate B ,4-Diaminotoluene Diamyl phthalate Diazepam Diazinon... 67,69 Dibenz[a,h]anthracene... 69,70 Dibenz[a,j]acridine Dibenzofuran Dibenzyl ether... 78,80 Dibenzyl phthalate ,2-Dibromo-3-chloropropane... 58,61,... 62,66 Dibromoacetic acid ,2-Dibromobenzene Dibromochloromethane... 58,61,62 1,2-Dibromoethane ,62 Dibromomethane... 58,61,62 α,α-dibromo-m-xylene ,3-Dibromoproprionic acid ,2-Dibromo-1,1,2, 2-tetrafluoroethane Dibutylchlorendate Dibutyl ether Dicamba Dichlofenthion Dichloroacetic acid ,4-Dichloroaniline ,2-Dichlorobenzene ,62,63,70,92 1,3-Dichlorobenzene ,62,63,70,92 1,4-Dichlorobenzene ,62,63,70,92 3,3'-Dichlorobenzidine cis-1,4-dichlorobutene... 62,63 trans-1,4-dichloro-2-butene ,3-Dichlorobiphenyl Dichlorodifluoromethane... 56,58,59,62 1,1-Dichloroethane... 57,61,62 1,2-Dichloroethane ,62 1,1-Dichloroethene ,62,63 cis-1,2-dichloroethene ,62,63 trans-1,2-dichloroethene ,62,63 1,1-Dichloro-1-fluoroethane Dichlorofluoromethane Dichloromethane ,4-Dichlorophenol ,6-Dichlorophenol Dichloroprop ,2-Dichloropropane... 57,61,62 1,3-Dichloropropane... 58,59,62 2,2-Dichloropropane... 58,61,62 1,1-Dichloro-2-propanone ,1-Dichloropropene ,62,63 cis-1,3-dichloropropene ,62,63 trans-1,3-dichloropropene 57,60,61,62,63 1,2-Dichloro-1,1,2,2-tetrafluoroethane. 56 2,2-Dichloro-1,1,1-trifluoroethane Dichlorvos... 67,69 Dicrotophos Dicyclohexylamine... 68,91 Dicyclohexyl phthalate ,1-Difluoroethane Dieldrin ,69 Diethylamine ,6-Diethylaniline N,N-Diethylaniline Diethylenetriamine Diethyl ether Diethyl phthalate Diethyl sulfide Diethyltryptamine ,4-Difluorobenzene... 57,58 Dihexyl phthalate Diisobutyl phthalate Diisopropylamine ,3-Diisopropylbenzene ,4-Diisopropylbenzene Diisopropyl ether (DIPE) Dimethoate p-dimethylaminoazobenzene ,4-Dimethylaniline ,6-Dimethylaniline ,12-Dimethylbenz[a]anthracene N,N-Dimethylbenzylamine ,2-Dimethylbutane ,3-Dimethylbutane Dimethylformamide ,6-Dimethylhept-5-enal (Melonal).. 78,80 2,2-Dimethylhexane ,3-Dimethyl-2-nitrobenzene ,3-Dimethylpentane ,4-Dimethylpentane α,α-dimethylphenethylamine ,4-Dimethylphenol Dimethyl phthalate Dimethyl sulfide ,5-Dimethylthiophene Dimethyltryptamine Di-n-butyl phthalate ,3-Dinitrobenzene ,6-Dinitro-2-methylphenol ,4-Dinitrophenol... 68,70 2,4-Dinitrotoluene... 59,69,70 2,6-Dinitrotoluene... 59,69,70 3,4-Dinitrotoluene Dinonyl phthalate Dinoseb ,4-Dioxane Dioxathion Diphenamid Diphenylamine Diphenylaniline ,2-Diphenylhydrazine Diphenyl isophthalate Diphenyl oxide... 78,80 Diphenyl phthalate Disulfoton... 67,69 cis-4,7,10,13,16,19-docohexaenoic acid methyl ester cis-13,16-docosadienoic acid methyl ester γ-dodecalactone... 78,80 Dodecanoic acid Dodecanol... 78,80 Dursban cis-11,14-eicosadienoic acid methyl ester Eicosane... 78,79 cis-5,8,11,14,17-eicosapentaenoic acid methyl ester cis-11,14,17-eicosatrienoic acid methyl ester www. v priced-ap.p /22/01, 9:41 AM

140 Compound Index Part No.... Page Part No.... Page Part No.... Page Part No.... Page cis-11-eicosenoic acid methyl ester cis-8,11,14-eicosenoic acid methyl ester Elaidic acid methyl ester Endosulfan I ,69 Endosulfan II ,69 Endosulfan sulfate ,69 Endrin ,69 Endrin aldehyde ,69 Endrin ketone EPN EPTC Erucic acid methyl ester Ethane... 93,95-98 Ethanol... 85,86,88,89,90 Ethion Ethoprop... 67,69 2-Ethoxyethanol (Cellosolve)... 88,89 bis(2-ethoxyethyl) phthalate... 68,69 Ethyl acetate... 78,80,85,90,93 Ethyl acrylate Ethylbenzene ,62,63,92,97 Ethyl butyrate... 78,80 Ethyl decanoate Ethyl disulfide Ethyl dodecanoate Ethylene... 93,95-98 Ethylenediamine Ethylene glycol... 88,89 Ethylene oxide Ethyl ether... 85,93 Ethyl heptanoate... 78,80,81 Ethyl hexadecanoate... 78,80 Ethyl hexanoate... 78,80 2-Ethylhexanoic acid... 68,84 2-Ethyl-1-hexanol... 83,88,89 bis-(2-ethylhexyl) adipate bis(2-ethylhexyl) phthalate... 68,70 Ethyl isovalerate... 78,80 Ethyl mercaptan... 94,95 Ethyl methacrylate Ethylmethyl sulfide Ethyl methanesulfonate Ethyl nonanoate... 78,80 Ethyl octadecanoate... 78,80 Ethyl octanoate Ethyl parathion Ethyl pentadecanoate... 78,80 Ethyl pentanoate... 78,80 α-ethylphenyl alcohol... 88,89 β-ethylphenyl alcohol... 88,89 Ethyl propionate... 78,80,90 Ethyl undecanoate... 78,80 Ethyl-tert-butyl ether (ETBE) Ethyl tetradecanoate... 78,80 2-Ethylthiophene Eugenyl acetate... 78,80 Eugenyl methyl ether... 80,81 Evernyl Famphur Fenamiphos Fenarimol Fenchyl acetate... 78,79 Fenitrothion Fensulfothion Fentanyl... 86,87 Fenthion Florazone... 78,80 Flunitrazepam Fluoranthene Fluorene... 69,70 Fluorobenzene... 58,62,63 Fluridone Fonofos Formic acid Frambione (raspberry ketone)... 78,80 Freon 11 (Trichlorofluoromethane) Freon 114 (1,2-Dichloro-1,1,2,2- tetrafluoroethane) Freon 12 (Dichlorodifluoromethane) D-Fructose D-Fructose (anomer) Fucitol Fufural... 78,80 Furfuryl alcohol... 88,89 Galactitol α-d-galactose Geranial Geraniol Geranyl acetate Geranyl butyrate Geranyl formate Geranyl-2-methyl valerate... 78,79 Geranyl tiglate Gesatamine Glucitol α-d-glucose β-d-glucose Glutethimide Glycidol... 88,89 3,7-Guaiadiene... 78,79 Heneicosane Heneicosanoic acid methyl ester Heptachlor ,69 Heptachlor epoxide ,69 2,2',3,3',4,4',6-Heptachlorobiphenyl Heptadec-1-ene... 78,79 Heptadecanoic acid methyl ester cis-10-heptadecenoic acid methyl ester 74 Heptane... 85,97 1-Heptanethiol Heptanoic acid... 76,90,92 1-Heptanol... 88,89 2-Heptanol... 88,89 3-Heptanol... 88,89 cis-4-hepten-1-ol... 88,89 trans-2-hepten-1-ol... 88,89 Hercolyn D (tetrahydro & dihydromethylabietate) Heroin Hexachlorobenzene... 66,69,70 Hexachloro-1,3-butadiene Hexachlorobutadiene ,62,63,70 2,2',4,4',5,6'-Hexachlorobiphenyl Hexachlorocyclopentadiene... 69,70 Hexachloroethane... 58,70 Hexachloropentadiene Hexadecanoic acid Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) Hexamethylphosphoramide ,6-Hexandiol Hexane n-hexane... 85,97,98 1-Hexanol... 88,89 2-Hexanol... 88,89 3-Hexanol... 88,89 Hexanol... 78,80,81 2-Hexanone Hexazinone Hexanthiol cis-2-hexen-1-ol... 88,89 cis-3-hexen-1-ol... 88,89 cis-3-hexen-1-ol cis-2-hexene trans-2-hexene Hexyl acetate Hexyl-2-ethylhexyl phthalate Hexylene glycol... 78,80 1-Hexyl mercaptan α-humulene Hydrogen Hydrogen sulfide... 94,95 Hydroxycitronellal... 78,80 4-Hydroxy-4-methyl-2-pentanone... 88,89 Ibogaine... 86,87 Ibuprofen Ideno[1,2,3-cd]pyrene Imipramine Indeno[1,2,3-c,d]pyrene Inositol Iodomethane... 58,62,63 Isoamyl salicylate Isoborneol... 78,80,83 Isobutane... 93,95-99 Isobutanol... 88,89,90 Isobutyl acetate... 78,80,90 Isobutyl acrylate Isobutylbenzene Isobutylene... 98,99 Isobutyl propionate Isobutyric acid... 76,90,92 Isocaproic acid... 76,90,92 Isodrin Isomenthone Isooctane (2,2,4-trimethylpentane) Isopentane... 93,96-99 Isophorone... 69,70 Isoprene... 97,99 Isopropanol... 85,86,88,89 Isopropyl acrylate Isopropylbenzene ,62,63,92,97 Isopropyl myristate... 78,80 p-isopropyltoluene ,62,63 Isovaleric acid... 76,90,92 IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC IUPAC Kelthane Ketamine Lauric acid methyl ester Leptophos Lidocaine Lignoceric acid methyl ester δ-limonene Limonene R(+)Limonene S(-)Limonene Linalool cis-linalool oxide trans-linalool oxide Linalyl acetate Linoleic acid methyl ester Linolelaidic acid methyl ester gamma-linolenic acid methyl ester Linolenic acid methyl ester Lorazepam LSD... 86,87 Malathion Mannitol D-Mannose MCPA MCPP (-)-Menthol (+)-Menthol Menthone Mephenytoin Merphos... 67,69 Mescaline Methacrylonitrile Methadone Methane... 93,95-98 Methanol... 85,86,88,89 Methaqualone Methoxychlor ,69 2-Methoxyethanol (Methyl cellosolve)... 88,89 bis(4-methoxyethyl) phthalate Methyl acetate Methyl acetylene Methyl acrylate... 58,90 4-Methylaminorex Methyl benzoate Methylbenzylamine Methyl-1-butanol (active amyl alcohol)... 88,89 2-Methyl-2-butanol (tert-amyl alcohol)... 88,89 3-Methyl-1-butanol (iso-amyl alcohol)... 88,89 2-Methyl-2-butene Methyl-1-butene... 97,99 3-Methyl-2-buten-1-ol... 88,89 2-Methyl-3-buten-2-ol... 88,89 2-Methylbutyl alcohol... 78,80 3-Methylbutyl alcohol... 78,80 Methyl butyrate... 78,80 3-Methylcholanthrene Methyl-cresol... 78,79 Methylcyclohexane Methylcyclopentane Methyl disulfide Methylene chloride... 58,51,62,85,93 Methyl ethyl ketone (MEK) Methylheptane Methylheptane Methylhexane Methylhexane Methyl mercaptan Methyl methacrylate... 58,90 Methyl methanesulfonate Methylnaphthalene Methylnaphthalene Methyl-para-cresol... 80,8 Methyl paraoxon Methyl parathion Methylpentane... 56,97 3-Methylpentane... 56,97 4-Methyl-2-pentanol... 88,89 4-Methyl-2-pentanone Methyl-1-pentene Methyl-1-pentene bis(4-methyl-2-pentyl) phthtalate Methyl phenol Methyl phenol α-methylphenyl alcohol... 88,89 2-Methyl-1-propanethiol Methyl-2-propanethiol Methyl propionate Methyl-1-propylmercaptan Methyl-1-propylmercaptan Methyl-2-propylmercaptan Methyl-2-pyrrolidine Methyl-tert-butyl ether (MTBE)... 56,58 2-Methylthiophene Methylthiophene Methyl-2,4,6-trinitrophenylnitramine (Tetryl) Metolachlor Metribuzin Mevinphos... 67,69 MGK Mirex Molinate Monocrotophos Musk T (ethylene brassylate)... 78,80 Myrcene Myrisoleic acid methyl ester Myristic acid methyl ester Naled Naphthalene... 56,58-61,62,63,70 1-Naphthylamine Naphthylamine Napropamide (-)-Neomenthol (+)-Neomenthol Neral ,82 cis-nerolidol... 78,79 trans-nerolidol... 78,79 Nervonic acid methyl ester Neryl acetate Nicotine... 85,86 2-Nitroaniline... 70,91 3-Nitroaniline Nitroaniline Nitroaniline... 70,91 Nitrobenzene... 58,59,70 Nitrogen... 95,96 Nitroglycerin Nitrophenol Nitrophenol Nitropropane N-Nitrosodimethylamine N-Nitrosodi-n-butylamine N-Nitrosodi-n-propylamine N-Nitrosodiphenylamine N-Nitrosopiperidine Nitrotoluene... 59,92 3-Nitrotoluene... 59,92 4-Nitrotoluene... 59,92 trans-nonachlor... 66,69 Nonadec-1-ene Nonadecane γ-nonalactone... 78,80 Nonanal... 82,83 n-nonane Nonanol... 88,89 n-nonylamine Norflurazon ,2',3,3',4,5',6,6'-Octachlorobiphenyl.. 69 cis-9-octadecanoic acid Octadecanoic acid Octahydro-1,3,5,7-tetranitro-1,3,5,7- tetrazocine (HMX) Octanal n-octane Octanoic acid Octanol... 88,89 Octanol... 78,80,82,83 Octyl acetate... 78,80,81 n-octylamine Di-n-octyl phthalate... 68,70 Oleic acid methyl ester Oxygen Palmitic acid Palmitic acid methyl ester Palmitoleic acid methyl eter PCP (Phencyclidine)... 86,87 Pentachlorobenzene ,2',3',4,6-Pentachlorobiphenyl www. 141 v priced-ap.p /22/01, 9:41 AM

141 Compound Index Part No.... Page Pentachloroethane Pentachloronitrobenzene... 66,70 Pentachlorphenol... 59,68-70 Pentadecanoic acid methyl ester cis-10-pentadecenoic acid methyl ester. 74 cis-1,3-pentadiene trans-1,3-pentadiene Pentafluorobenzene Pentafluoroethane n-pentane Pentane ,3-Pentanedione (acetyl propionyl)... 78,80 1-Pentanethiol Pentanol... 88,89 2-Pentanol... 88,89 3-Pentanol... 88,89 1-Pentene cis-2-pentene... 97,99 trans-2-pentene... 97,99 2-Penten-1-ol... 88,89 1-Penten-3-ol... 88,89 1-Pentyl mercaptan cis-permethrin... 66,69 trans-permethrin... 66,69 Perthane Perylene-d α-phellandrene β-phellandrene Phenacetin Phenanthrene Phenanthrene-d Phenethylalcohol Phenobarbital Phenol Phenoxyethanol... 88,89 Phenylethylacetate... 78,79 sec-phenylethyl alcohol Phenylethylamine Phenyl ethyl cinnamate... 78,80 Phenylethyltiglate Phorate Phosmet Phosphamidon Picloram Picoline α-pinene β-pinene Piperazine Prebane Procaine Prometon Prometryne Pronamid... 69,70 Propachlor... 66,69 Propadiene... 95,98,99 Propane... 93, Propanethiol Propanethiol n-propanol... 85,86,88,89 Propazine Propen-1-ol (Allyl alcohol)... 88,89 Propionic acid... 76,90,92 Propionitrile... 58,91 n-propylbenzene ,62,63,92,97 Propylene... 93,95-99 Propylene glycol... 88,89 1-Propyl mercaptan Propyl mercaptan Propyn-1-ol (Propargyl alcohol)... 88,89 Propyne Pyrazole Pyrene... 69,70 Pyrene-d Pyridazine Pyridine... 85,91 Pyrimidine Rhamnitol Ribitol D-Ribose anomers Ronnel Rosatol (rosetone)... 78,80 Rose oxide, cis-rose Rose oxide, trans-rose Sabinene Simazine... 67,69 Simetryn Stearic acid Stearic acid methyl ester Stirophos Styrene ,62,63,92,97 Sulfotepp Sulfur dioxide Part No.... Page Sulfur hexafluoride ,4,5-T Talbutal TCP (Tenocyclidine) Tebuthiuron TEPP Terbacil Terbufos... 67,69 α-terpinene γ-terpinene α-terpineol Terpinen-4-ol Terpinolene Terrazole... 66,69 1,2,4,5-Tetrachlorobenzene ,1,1,2-Tetrachloroethane... 58,61,62 1,1,2,2-Tetrachloroethane ,62 Tetrachloroethene ,62,63 Tetrachloro-m-xylene ,69 2,3,4,6-Tetrachlorophenol Tetrachlorvinphos Tetradecane Tetradecanoic acid Tetraethylenepentamine ,1,1,2-Tetrafluoroethane ,1,2,2-Tetrafluoroethane Tetrahydrofuran... 58,85 Tetrahydrothiophene Thionazin Thiopental Thiophene α-thujone... 78,79,82 β-thujone Tillam Tokuthion Toluene ,62,63,85,92,97,99 Toluene-d Tonalid... 78,80 2,4,5-TP Triadimefon Triazolam Tribromoacetic acid Trichloroacetic acid ,2,3-Trichlorobenzene ,62,63 1,2,4-Trichlorobenzene ,62,63,70 2,4,5-Trichlorobiphenyl ,1,1-Trichloroethane ,62 1,1,2-Trichloroethane ,62 Trichloroethene ,62,63 Trichloroethylene Trichlorofluoromethane... 56,58,61,62 Trichloronate ,4,5-Trichlorophenol ,4,6-Trichlorophenol ,2,3-Trichloropropane... 58,61,59,62 1,1,2-Trichloro-1,2,2-trifluoroethane Tricosanoic acid methyl ester Tricyclazole Tridecane Tridecanoic acid methyl ester Triethylamine Triethylenetetramine ,1,1-Trifluoroethane Trifluoromethane Trifluorotoluene... 62,63 α, α, α-trifluorotoluene Trifluralin... 66,69 1,2,3-Trimethylbenzene ,2,4-Trimethylbenzene ,62,63,97 1,3,5-Trimethylbenzene ,62,63,97 3,5,5-Trimethylhexanol... 78,80,81 2,3,4-Trimethylpentane Trimipramine... 86,87 1,3,5-Trinitrobenzene ,4,6-Trinitrotoluene Tri-o-cresyl phosphate Triphenylphosphate Undecanoic acid methyl ester Undecanol Valeric acid (pentanoic acid)... 76,90,92 γ-valerolactone Vernolate Vertenex Vinyl chloride ,62,63 4-Vinylcyclohexene-1 (dimer) m-xylene ,62,63,92,97 o-xylene ,62,63,92,97 p-xylene ,62,63,92,97 D-Zylose www. Trademarks Agilent Trademarks The following are trademarks of Agilent Technologies Accubond Autostart Cyclodex-B TM Connex TM DB TM DB-CVU DB-Dioxin TM DB-ProSteel TM Durabond Durawax EVIDEX TM GS-Q GS-Alumina Megabore Microbore Minibore µpage µsil Measuring Flow the Intelligent Way TM ADM Model flowmeters manufactured under U.S. Patent 5,460,038 DC, UC, S, QF, 007, PE, NB, and FFAP are not registered trademarks of Agilent Technologies. Other Trademarks Product Company Al 2O 3lKCl Chrompack Al 2O 3Na 2SO 4 Chrompack Aroclor Monsanto AT Alltech Associates BP & BPX SGE Carbopack Supelco Carbosieve Supelco Carbowax Union Carbide Corporation CC C+C Column Technologies Chirasil-Val Alltech Associates Chromosorb Johns Manville Corporation CP Supelco CP-Sil Chrompack Int'l., BV CP-WAX Chrompack Int'l., BV Decthal Diamond Shamrock Corporation Drierite W.A. Hammond Co. Envi-18 Supelco Florisil Floridin Corp. Flow Inverter R&D Separations Fluorolube Occidental Chemical Corporation Focus Liner SGE GASTIGHT Hamilton Company Hall Tracor Instruments HayeSep Hayes Separations, Inc. HP Hewlett-Packard Company Innowax Innophase Labcraft Curtin Matheson Scientific, Inc. Lexan General Electric Company LIPODEX Macherey-Nagel GmbH & Co. KG Megaliner R&D Separations MICROLITER Hamilton Company Molesieve 5Å Chrompack MXT Restek Corp. NiCat OI Analytical NON-PAKD Alltech Associates, Inc. Nukol Supelco Nupro Nupro Co. Omegawax Supelco OPTIC Tekmar - Dohrmann, Cincinnati, OH OV Ohio Valley Specialty Chemical Company Parker Parker Hannifin Co. PE Perkin Elmer Petrocol Supelco PLT-5A Quadrex Polywax Petrolite Specialty Polymers Group Porapak Waters PoraPLOT Chrompack PTE Supelco RH Chromatix RSL RSL Rt & Rtx Restek Corp. Rt-ßDEXm Restek Corp. Rt-Msieve Restek Corp. Scotty II Scott Specialty Gases SE General Electric SepPak Millipore Corp. Silar Silar Snoop Nupro Company SP & SPB Supelco Stabilwax Restek Corp. SUPELCOWAX Supelco SUPEROX Alltech Associates, Inc. Sup-Herb Supelco Swagelok Crawford Fitting Co. Teflon E.I du Pont Nemours & Company, Inc. Tenax Enka Research Institute Arnhem Ultra Hewlett-Packard Company Vespel E.I. du Pont Nemours & Company, Inc. VOCOL Supleco v priced-ap.p /15/01, 12:55 AM

142 Part Number Index Part No.... Page , , , , , , ,56,94, , H , , , , A , , A , , ,42, , , , , , , , , ,21, , , , , , , ,40 Part No.... Page Part No.... Page Part No.... Page Part No.... Page Part No.... Page , , , ,20, , ,61, , , , E A , ,19,68, , E A , , , E E A A , AE , , , ,42,61, , , , , , , , , , , , H , ,28, , , , ,29, , , , ,27, , , ,25,64, , , , , E C , , , E , , , E , , , , A , ,22, , ,22,70, , , , , , , , ,40,73, ,40, , , , , , , , , , E , A D F , , , , E G C F , , ,19, B E G HC , , , , , , , , , , , , , , , , , , www , ,21, , E , D F , , , B E , D , , , E , , , , , , , , A , , , , , , , , , , , , , ,59, A J... 19, K J B D J K J H H H HB HJ , A ,59, , H v priced-ap.p /22/01, 9:42 AM

143 Part Number Index Part No.... Page J K J B D J K J H H HB J , , , A A , A E , , , , , , , H , , ,99 Part No.... Page Part No.... Page Part No.... Page Part No.... Page Part No.... Page HFM HFM-570-PC HFM , HFM-650-FC , HFM HMF , , HMT HMT HT , HT HT-2-SS H HT , HT , ,131 HT-4-SS HVF HVF IOT-2-HP IOT-4-HP LCOT LCOT LGMT-2-HP LGMT-4-HP , LIOT LIOT MC , MC , MSR MSR-1X , MSR MT MT120-2-D B MT120-2-S MT MT120-4-D MT120-4-S MT MT200-2-D MT200-2-S , MT MT200-4-D MT200-4-S MT-2-SS MT MT400-2-D MT400-2-S MT MT400-4-D MT400-4-S MT-4-SS OT-2-SS OT OT OT-4-SS R2D R2D R2D R2D R2D2-I-P R2D2-P RBC RBC-2-D RBC RBC-4-D RBC-D-P RBC-P A RDT B RDT B RFT D RFT F RFT G ACR ,116 RMP BACR ,117 RMSH A BHT ,117 RMSH B BHT ,117 RQC D BHT ,117 RQC F BMSR RQC-P G BMT RTC BMT RTC A BMT RTC-I-P B BOT RTC-P E BOT SGR F BOT SL G COT SL COT SSR HP COT-R , 116 SSR V GC SSR GC UMC H GC UMC ,113, GC UMC ,111, GC UMC , GMSR UMC E GMT-2GC-HP V GMT-2-HP E GMT-4GC-HP V GMT-4-HP HCRMS HFM HFM HFM HFM made by elves 144 www. v priced-ap.p /22/01, 9:42 AM

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