Model HT Microplate Orbital Shaker With Manual Adjustment Knobs. Product Manual
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1 Microplate Orbital Shaker With Manual Adjustment Knobs Big Bear, Inc. Pleasanton, CA USA Tel: Fax: Web:
2 Manual Version: 1.00 Manual Date: Filename: HT91002Manual.pdf Copyright 2003 Big Bear, Inc. Page 2
3 Description Congratulations on your purchase of Big Bear s Microplate Orbital Shaker. This precision laboratory instrument will provide years of reliable operation for you. The HT Microplate Orbital Shaker is designed to provide an orbital shaking motion for a single microplate, keeping liquids either gently stirred or fully mixed and in suspension. Its thin profile and small footprint dimension is ideal for use in crowded laboratory space. HT Microplate Orbital Shaker with Manual Knob Adjustments Page 3
4 Features Rotationally shakes a single microplate. 1 mm orbital motion. Small enough not to spill liquids at high speed. 2 selectable & adjustable speeds. Low stirring speed to ultra-vigorous vortex speeds for viscous liquids. 24 VDC power for safe and easy integration with other automation. Green LED power indicator, two blue LEDs for speed selection indicator. Solid aluminum block base with rubber feet provides stable platform. No walking Cold running motor allows continuous operation, weeks at a time. Small number of precision components for extreme reliability over years of use. Spring clips secure microplate; easy on and off. Specifications Model HT Microplate Orbital Shaker Orbital Motion Speed Power Required 1 mm diameter, circular shape, constant everywhere on well plate 60 RPM to 3500 RPM, 2 separately adjustable potentiometers for two independent speeds. Three position panel switch for Speed A or B and off. 24 VDC, 225 ma internally, VAC Hz AC power adapter. Connection for Power Power jack for supplied VAC power adapter. Base Dimension Shaker Platform Dimension Height Weight 3.350" (85mm) width, 7.290" (185.2mm) length 3.650" (92.7mm) width, 5.300" (134.6mm) length, centered on top of base. Secures a single 96 or 384 micro well plate with SBS footprint " (40.4mm) height from bottom of urethane no-slip surface to top surface of shaker table platform " (53.6mm) height from bottom of urethane surface to the top surface of a standard well plate pounds (1.08 kg) Operating Temperature 0 F to 120 F (-17.5 C to 49.0 C), non-condensing, RH to 90% Start-up Time Mixing Speed Indicators Material Warranty 1.5 seconds to set speed Speed varies based on your liquid viscosity. For 96 well plates water fully mixes in each microplate well at 1300 RPM, 2000 RPM for 384 well plates. Green LED for power indicator, two blue LEDs at rear, two green LEDs on front panel indicating Speed A or Speed B activation. Aluminum, nickel plated, stainless steel hardware, white Delrin clips. Two year limited replacement warranty. Page 4
5 Set-up Instructions Included in your package is: HT Microplate Orbital Shaker VAC Hz Power Adapter with cables CDROM with this manual in pdf format. Remove from packaging and place base flat on a clean table. There are no operator accessible controls internally. Simply apply power using the supplied AC adapter, select a speed using the rocker switch, and adjust to any speed. Well plates snap directly onto the shaker table surface. Controls Overview This photo shows the main control and power connections on the shaker. This unit has two individual speeds available. You may select which speed is used by the small black three-position rocker switch on the control panel. With the rocker to the left, Speed A is selected, and to the right Speed B is selected. When the switch is at the center position, the unit will not run or will stop if currently running. On the HT model there are two lockable vernier potentiometers allowing adjustment of the two separate shaking speeds. The speed adjustment potentiometers control the two speed conditions. Each pot can control the entire range of 60 RPM through 3500 RPM. Therefore, the difference between the Speed A and Speed B shaking speeds can vary by any amount, including a zero difference. You may adjust the pot while the unit is running. Adjusting the pots clockwise will provide faster speeds, while adjustment counter-clockwise results in slower speeds. Speed A Adjustment Speed A Indicator Green LED Three Position Speed Switch Speed B Indicator Green LED Speed B Adjustment Page 5
6 24 VDC Power Plug Power Indicator Green LED Speed A Indicator Blue LED Speed B Indicator Blue LED VAC Hz Power Adapter Rear View of HT Showing Power Connection and LED Indicators On the rear of the unit, you will find the jack for plugging in the 24 VDC plug from the AC adapter. When this power supply is plugged in, the green power LED on the rear of the unit will illuminate. It will always be on, even if the front panel speed selection switch is in the center off position. When either speed is selected using the rocker switch, both the blue LED at the rear of the unit and the green LED on the front panel will illuminate. Page 6
7 Speed Adjustment Use the following table for a rough guide to the speed selection available on the vernier potentiometer knobs. Knob Adjustment Speed Table Dial Adjustment Indication Shaker RPM Application Information The HT Microplate Orbital Shaker is capable not only of gently stirring liquids, but also of vigorously mixing liquids, and maintaining suspension, for each and every well. Mixing occurs at differing speeds based on the viscosity of the liquid. The orbital motion is of a small enough diameter that liquid motion is contained within the well, and no spillage occurs even at the higher speeds. Gentle stirring is useful for preventing surface skimming, while mixing completely disperses one soluble liquid within another, or maintains suspension of particles within a liquid while precluding settling. 96 well-plate water mixing occurs at approximately 1250 RPM, and occurs within 2 seconds of activating that speed. 384 well-plate water mixing occurs at approximately 2000 RPM. Page 7
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