GMOD powering LanzaTech. Asela W. S. Dassanayake Bioinformatics Research Scientist Model Systems Group
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1 GMOD powering LanzaTech Asela W. S. Dassanayake Bioinformatics Research Scientist Model Systems Group 2016 GMOD Meeting Indiana University, Bloomington, Indiana, United States June 30 July 1, LanzaTech. All rights reserved. 1
2 The outline LanzaTech - Who we are and what we do How GMOD is powering the information flow at LanzaTech Proposing a Synthetic Biology platform? 2
3 Ed Hawkins, Climate scientist, National Centre for Atmospheric Science, University of 3
4 Carbon smart world Remaining: 1000 GtCO : 1900 GtCO 2 65% of 2 carbon budget: USED Must stay in the ground 4
5 Proprietary Acetogenic Biocatalyst Worlds first autotrophs Acetogenic bacterium with ability to utilize sole energy and carbon source CO CO+H 2 or CO+CO 2 +H 2 CO 2 +H 2 gases as LanzaTech has developed a proprietary strain of Clostridium autoethanogenum 1 Obtained by extensive selection program, having improved characteristics High gas uptake and ethanol production rates Fast growth on defined minimal media Non-sporulating and non-motile 5
6 1 Organism, over 20 Products 1 Organism, over 25 Products CO/H 2 Acetyl-CoA Pyruvate Fatty Acids, Terpenoids Aromatics Biodiesel (FAEE) 3-Hydroxypropionate Jet Fuel (3-HP) Isoprene Aromatics Branched-chain Amino Acids Acetoin 2-Butanol 1-Propanol 1,2-Propanediol Discovery Continuous lab-scale fermentation Partnerships 1,3-Butadiene Biopolymers Piloting >50% selectivity at lab scale Scaled Up Butyrate 1-Butanol 1,3-Butanediol (1,3-BDO) Methyl Ethyl Ketone (MEK) Succinate Lactate Butylene Ethanol 3-Hydroxybutyrate Isopropanol (3-HB) Acetone Acetate 2,3-Butanediol (2,3-BDO) 6
7 The LanzaTech Process Propanol Butanol Hydrocarbon Fuels Butanediol Gas Feed Stream Ethanol Gas Reception Compression Fermentation Recovery Product Tank Nylon 6,6 (from Adiponitrile/HDMA) Acrylonitrile Butadiene Styrene (ABS)
8 A sensible path We use: Industrial Waste Gas MSW Waste Biomass We do not use: Land Corn For Sugar People Cane 8
9 3 Commercial projects underway Strong Pipeline and Committed Partners Commercial Production 2017
10 ArcelorMittal, Gent Ground Works Started October 2015 Gas Testing Station Produces Ethanol January 2016 Connection to Steel Mill Gas Lines March
11 1 Organism, over 20 Products 1 Organism, over 25 Products CO/H 2 Acetyl-CoA Pyruvate Fatty Acids, Terpenoids Aromatics Biodiesel (FAEE) 3-Hydroxypropionate Jet Fuel (3-HP) Isoprene Aromatics Branched-chain Amino Acids Acetoin 2-Butanol 1-Propanol 1,2-Propanediol Discovery Continuous lab-scale fermentation Partnerships 1,3-Butadiene Biopolymers Piloting >50% selectivity at lab scale Scaled Up Butyrate 1-Butanol 1,3-Butanediol (1,3-BDO) Methyl Ethyl Ketone (MEK) Succinate Lactate Butylene Ethanol 3-Hydroxybutyrate Isopropanol (3-HB) Acetone Acetate 2,3-Butanediol (2,3-BDO) 11
12 Information flow at LanzaTech BIOENGINEERING BIOCHEMISTRY, PATHWAY ANALYSIS FERMENTATION BIOINFORMATICS, STATISTICS, MODELING GENOMIC, TRANSCRIPTOMIC, PROTEOMIC, METABOLOMIC DATA 12
13 GMOD at LanzaTech External Collaborators 13
14 Apollo at LanzaTech - Internal 14
15 Roshomon Transcriptional Regulator Methyl accepting chemotaxis protein Transcriptional Regulator Iron-Sulphur binding cluster Uncharacterised ORF 15
16 Tuff-t 16
17 Tuff-t 17
18 1 Organism, over 20 Products 1 Organism, over 25 Products CO/H 2 Acetyl-CoA Pyruvate Fatty Acids, Terpenoids Aromatics Biodiesel (FAEE) 3-Hydroxypropionate Jet Fuel (3-HP) Isoprene Aromatics Branched-chain Amino Acids Acetoin 2-Butanol 1-Propanol 1,2-Propanediol Discovery Continuous lab-scale fermentation Partnerships 1,3-Butadiene Biopolymers Piloting >50% selectivity at lab scale Scaled Up Butyrate 1-Butanol 1,3-Butanediol (1,3-BDO) Methyl Ethyl Ketone (MEK) Succinate Lactate Butylene Ethanol 3-Hydroxybutyrate Isopropanol (3-HB) Acetone Acetate 2,3-Butanediol (2,3-BDO) 18
19 Proposing an Integrated Synthetic Biology platform User data organization and management BLAST Interface Database Searching Literature searches and bibliography Genome browser Data Visualization Synthetic Biology CAD with SBML/SBOL 19
20 Integrated Synthetic Biology platform 20
21 More Users? Funding? 21
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