PARTICIPANT STATISTICS Chemistry Q Comprehensive Chemistry. Specimen 1 Specimen 2 Specimen 3 Specimen 4 Specimen 5 Range & Type.

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1 PARTICIPANT STATISTICS Chemistry Q Comprehensive Chemistry Specimen 1 Specimen 2 Specimen 3 Specimen 4 Specimen 5 Name Line No. No. of Labs Alpha-fetoprotein Roche Elecsys S luminometric S Whole Population S S S S S S S S S S S S S Amylase and Instrument Abbott Aeroset/Architect & Abbott Architect c, ci, i P P P P P Beckman Olympus & Beck Olym AU 400/600/ P P P P P Beckman Olympus & Beckman AU P P P P P Ortho Vitros & Ortho Vitros 3600, P P P P P Ortho Vitros & Ortho Vitros not DT or ECi P P P P P Roche Cobas & Roche e/c, 1XX, X000, Elec series P P P P P Siemens Dimension & Siemens Dimension EXL P P P P P Siemens Dimension & Siemens Dimension Xpand P P P P P Abbott Aeroset/Architect P P P P P Beckman Olympus P P P P P Ortho Vitros P P P P P Roche Cobas P P P P P Siemens Dimension P P P P P Standardized methods P P P P P All Beckman P P P P P Vitros and related P P P P P Roche and related P P P P P Siemens and related P P P P P Whole Population P P P P P Bilirubin, Direct Comprehensiv e and Instrument Abbott acid diazo & Abbott Architect c, ci, i C C C C C Beckman Coulter & Beck Coult Unicel DXC C C C C C Beckman Olympus & Beck Olym AU 400/600/ C C C C C Beckman Olympus & Beckman AU C C C C C Ortho Vitros & Ortho Vitros 3600, C C C C C Siemens Dimension DBI & Siemens Dimension EXL C C C C C Abbott acid diazo C C C C C Beckman Coulter C C C C C Beckman Olympus C C C C C Ortho Vitros C C C C C Roche acid diazo C C C C C

2 Siemens Dimension DBI C C C C C Acid diazo methods C C C C C Diazonium ion methods C C C C C Other C C C C C Whole Population C C C C C Cortisol Beckman Coulter Access P P P P P Roche Elecsys P P P P P Immuno-not FPIA P P P P P luminometric P P P P P Whole Population P P P P P Creatine Kinase, Total and Instrument Abbott & Abbott Architect c, ci, i P P P P P Beckman Olympus & Beck Olym AU 400/600/ P P P P P Beckman Olympus & Beckman AU P P P P P Ortho Vitros & Ortho Vitros 3600, P P P P P Ortho Vitros & Ortho Vitros not DT or ECi P P P P P Roche Cobas & Roche e/c, 1XX, X000, Elec series P P P P P Siemens Dimension & Siemens Dimension EXL P P P P P Siemens Dimension IFCC & Siemens Dimension EXL P P P P P Abbott P P P P P Beckman Coulter P P P P P Beckman Olympus P P P P P Ortho Vitros P P P P P Roche Cobas P P P P P Siemens Dimension P P P P P Siemens Dimension IFCC P P P P P IFCC standardized methods P P P P P Vitros and related P P P P P Whole Population P P P P P Gamma-Glutamyltransferase (GGT) and Instrument Beckman Olympus & Beck Olym AU 400/600/ C P P P P Beckman Olympus & Beckman AU C P P P P Roche Cobas & Roche e/c, 1XX, X000, Elec series C P P P P Siemens Dimension & Siemens Dimension EXL C P P P P Beckman Coulter C P P P P Beckman Olympus C P P P P Ortho Vitros C P P P P Roche Cobas C P P P P Siemens Dimension C P P P P Standardized methods C P P P P Abbott and Beckman Coulter C P P P P Vitros and related C P P P P Siemens and related C P P P P Whole Population C P P P P

3 Human Chorionic Gonadotropin (hcg) and Instrument Beckman Coulter Access & Beckman Coulter Access S S S S S Ortho Vitros ECi & Ortho Vitros 3600, S S S S S Roche Elecsys & Roche e411/e601/e170/e S S S S S Roche HCG Stat & Roche e411/e601/e170/e S S S S S Roche HCG+b & Roche e411/e601/e170/e S S S S S Siemens Advia & Siemens Centaur/Centaur CP S S S S S Siemens Dimension & Siemens Dimension EXL S S S S S Siemens Dimension LOCI & Siemens Dimension EXL S S S S S Siemens Immulite & Siemens Immulite S S S S S Tosoh beta-hcg & Tosoh AIA S S S S S Tosoh beta-hcg & Tosoh AIA ST S S S S S Beckman Coulter Access S S S S S Ortho Vitros ECi S S S S S Roche Elecsys S S S S S Roche HCG Stat S S S S S Roche HCG+b S S S S S Siemens Advia S S S S S Siemens Dimension S S S S S Siemens Dimension LOCI S S S S S Siemens Immulite S S S S S Tosoh Total b-hcg S S S S S Tosoh beta-hcg S S S S S IFCC standardized S S S S S Total Beta methods S S S S S Vitros and related S S S S S Siemens Immulite and related S S S S S Whole Population S S S S S Iron and Instrument Abbott Architect & Abbott Architect c, ci, i P P P P P Beck Oly tripyridyltriazine & Beck Olym AU 400/600/ P P P P P Ortho Vitros & Ortho Vitros 3600, P P P P P Roche Cobas & Roche Cobas P P P P P Roche Cobas & Roche e/c, 1XX, X000, Elec series P P P P P Siemens Dimension & Siemens Dimension EXL P P P P P Abbott Architect P P P P P Beck Oly tripyridyltriazine P P P P P Beckman Coulter P P P P P Beckman Olympus Ferene P P P P P Ortho Vitros P P P P P Roche Cobas P P P P P Siemens Dimension P P P P P Ferene-based P P P P P Ferrozine-based P P P P P Tripyridyltriazine (TPTZ) P P P P P All other methods P P P P P Whole Population P P P P P Lactic Acid and Instrument

4 Ortho Vitros & Ortho Vitros 3600, C P P P P Siemens Dimension & Siemens Dimension EXL C P P P P Ortho Vitros C P P P P Siemens Dimension C P P P P luminometric C P P P P Whole Population C P P P P Lactate Dehydrogenase, Total (LDH) and Instrument Beckman Olympus & Beck Olym AU 400/600/ P P P P P Beckman Olympus & Beckman AU P P P P P Ortho Vitros & Ortho Vitros not DT or ECi P P P P P Siemens Flex LDI & Siemens Dimension EXL P P P P P Beckman Coulter P P P P P Beckman Olympus P P P P P Ortho Vitros P P P P P Roche Cobas P P P P P Siemens Dimension P P P P P Siemens Flex LDI P P P P P IFCC Standardized P P P P P Other no cofactor P P P P P Siemens and related P P P P P Vitros and related P P P P P Whole Population P P P P P Lipase and Instrument Abbot Architect & Abbott Architect c, ci, i P P P P P Beckman Olympus & Beck Olym AU 400/600/ P P P P P Beckman Olympus & Beckman AU P P P P P Ortho Vitros & Ortho Vitros 3600, P P P P P Siemens Dimension & Siemens Dimension EXL P P P P P Siemens LIPL, liquid & Siemens Dimension EXL P P P P P Abbot Architect P P P P P Beckman Olympus P P P P P Ortho Vitros P P P P P Roche Cobas Integra P P P P P Siemens Dimension P P P P P Siemens LIPL, liquid P P P P P Enzymatic/colorimetric P P P P P Colorimetric P P P P P Whole Population P P P P P Magnesium mg/dl and Instrument Abbott & Abbott Architect c, ci, i P P P P P Beckman Coulter & Beck Coult Unicel DXC P P P P P Beckman Olympus & Beck Olym AU 400/600/ P P P P P Beckman Olympus & Beckman AU P P P P P Ortho Vitros & Ortho Vitros 3600, P P P P P Ortho Vitros & Ortho Vitros not DT or ECi P P P P P Roche Cobas & Roche Cobas P P P P P

5 Roche Cobas & Roche e/c, 1XX, X000, Elec series P P P P P Siemens Dimension & Siemens Dimension EXL P P P P P Siemens Dimension & Siemens Dimension Xpand P P P P P Abbott P P P P P Beckman Coulter P P P P P Beckman Olympus P P P P P Ortho Vitros P P P P P Roche Cobas P P P P P Siemens Dimension P P P P P Calmagite-based P P P P P Arsenazo P P P P P Magon (Xylidyl Blue)-based P P P P P All other methods P P P P P MTB (methylthymol blue) P P P P P Whole Population P P P P P Magnesium meq/l Whole Population P P P P P Thyroid Stimulating Hormone and Instrument Abbott CMIA & Abbott Architect c, ci, i S S S S S Beckman Coulter Access & Beckman Coulter Access S S S S S Beckman Coulter Access & Beckman Coulter DxI S S S S S MP Biomedicals & All gamma counters S S S S S Ortho Vitros & Ortho Vitros 3600, S S S S S Roche Elecsys & Roche e/c, 1XX, X000, Elec series S S S S S Roche Elecsys & Roche e411/e601/e170/e S S S S S Siemens Advia & Siemens Centaur/Centaur CP S S S S S Siemens Dimension & Siemens Dimension Xpand S S S S S Siemens Dimension LOCI & Siemens Dimension EXL S S S S S Siemens Immulite 3rd gen & Siemens Immulite S S S S S Tosoh AIA & Tosoh AIA S S S S S Tosoh AIA & Tosoh AIA ST S S S S S Abbott CMIA S S S S S Beckman Coulter Access S S S S S MP Biomedicals S S S S S Ortho Vitros S S S S S Roche Elecsys S S S S S Siemens Advia S S S S S Siemens Dimension S S S S S Siemens Dimension LOCI S S S S S Siemens Immulite 3rd gen S S S S S Tosoh AIA S S S S S Standardized methods S S S S S Tosoh and RIA S S S S S Siemens non Immulite S S S S S Whole Population S S S S S Thyroxine, Free (FT4) and Instrument Abbott CMIA & Abbott Architect c, ci, i S S S S S Beckman Coulter Access & Beckman Coulter Access S S S S S Beckman Coulter Access & Beckman Coulter DxI S S S S S

6 MP Biomedicals & All gamma counters S S S S S Ortho Vitros & Ortho Vitros 3600, S S S S S Roche Elecsys & Roche e/c, 1XX, X000, Elec series S S S S S Roche Elecsys & Roche e411/e601/e170/e S S S S S Siemens Advia/Centaur/CP/XP & Siemens Centaur/Centaur CP S S S S S Siemens Dimension & Siemens Dimension Xpand S S S S S Siemens Dimension LOCI & Siemens Dimension EXL S S S S S Tosoh AIA & Tosoh AIA S S S S S Abbott CMIA S S S S S Beckman Coulter Access S S S S S MP Biomedicals S S S S S Ortho Vitros S S S S S Roche Elecsys S S S S S Siemens Advia/Centaur/CP/XP S S S S S Siemens Dimension S S S S S Siemens Dimension LOCI S S S S S Siemens Immulite (1-step) S S S S S Tosoh AIA S S S S S Standardized methods S S S S S Vitros and related S S S S S Siemens non-immulite S S S S S All Immulite S S S S S Whole Population S S S S S Thyroxine, Total (TT4) and Instrument Abbott CMIA & Abbott Architect c, ci, i P P P P P Beckman Coulter Access & Beckman Coulter Access P P P P P Beckman Coulter Access & Beckman Coulter DxI P P P P P Siemens Dimension & Siemens Dimension EXL P P P P P Abbott CMIA P P P P P Beckman Coulter Access P P P P P Roche Elecsys P P P P P Siemens Dimension P P P P P Tosoh AIA P P P P P Standardized methods P P P P P Beckman Coulter and related P P P P P Whole Population P P P P P Triiodothyronine, Total (TT3) and Instrument Beckman Coulter Access & Beckman Coulter Access S S S S S Beckman Coulter Access & Beckman Coulter DxI S S S S S MP Biomedicals & All gamma counters S S S S S Roche Elecsys & Roche e/c, 1XX, X000, Elec series S S S S S Beckman Coulter Access S S S S S MP Biomedicals S S S S S Roche Elecsys S S S S S Siemens Advia S S S S S Standardized methods S S S S S Beckman Coulter and related S S S S S Whole Population S S S S S

7 T-Uptake % of Total and Instrument Beckman Coulter Access & Beckman Coulter Access S S S S S Siemens Dimension & Siemens Dimension EXL S S S S S Beckman Coulter Access S S S S S Siemens Dimension S S S S S Beckman Coulter and Vitros S S S S S Standardized methods S S S S S Whole Population S S S S S T-Uptake Ratio to Normal luminometric S S S S S Whole Population S S S S S

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