Labgard Class II, Type B2 Laminar Flow Biological Safety Cabinet. Operation & Maintenance Manual

Transcription

1 Labgard Class II, Type B2 Laminar Flow Biological Safety Cabinet Models NU /600 NU E/600E NU G/600G Bench/Console Operation & Maintenance Manual (Series 20 and Higher) June, 1999 (115 VAC Only) (115 VAC Only) (115 VAC Only) Manufactured by: NuAire, Inc Fernbrook Lane Plymouth, Minnesota USA Toll Free In MN OM0082

2 Fax OM0082 2

3 Congratulations! You have just purchased one of the finest Laminar Flow Biological Safety Cabinets available. With proper care, maintenance (certification), and laboratory procedure, this cabinet will give you years of product and personnel protection from particulate contaminants as prescribed in National Sanitation Foundation (NSF) Standard No. 49. Please read this manual carefully to familiarize yourself with proper installation, maintenance and operation of the cabinet. Appendix A lists Training Aid Materials, Films, Pamphlets and Books that may assist you in the proper application/procedure of the cabinet. Acknowledgment NuAire, Inc. acknowledges that some material in this manual reflects information supplied by the National Institutes of Health personnel both in verbal and written specifications. In particular, NuAire acknowledges that information in Section 8 was obtained from the following sources: 1. Technical Report No. FPS Prepared by Dow Chemical Co., for the National Cancer Institute, May 1, Stolar MH, Power LA, Vielo CS: Recommendations for handling cytotoxic drugs in hospitals. Am J Hosp Pharm 1983;40: OM0082 3

4 Anderson R.W., Director of Pharmacy, University of Texas, M.D. Anderson Hospital and Tumar Institute at Houston. OM0082 4

5 ABOUT THIS OPERATION & MAINTENANCE MANUAL The information contained in this manual is intended to reflect our current production standard configuration model along with the more frequently purchased options. Any unique additions/modifications/shop drawings are appended in the back flap of this manual, along with any modifications and/or additions to procedures as outlined in this manual. A copy of the original factory test report is also appended to this manual. In case this manual and/or test report is lost or misplaced, NuAire retains a copy in our files. A replacement copy can be obtained by calling or writing NuAire, Inc. stating the model number and serial number and a brief description of the information desired. OM0082 5

6 Labgard Class II, Type B2 Laminar Flow Biological Safety Cabinet Models NU /600 NU E/600E / NU G/600G Operation & Maintenance Manual TABLE OF CONTENTS Section No General Description Section No Construction Features Section No Models & Options Section No Test Performance & Procedures Section No Warranty Section No Shipments Section No Installation Instructions Location Set-up Instructions Certification Section No Operating the NU Electronic Airflow Control System Operating Guidelines Operating Sequence Antineoplastic Decontamination Procedure Section No General Maintenance Fluorescent Lamp Replacement HEPA Filter/Motor Replacement Sliding Window Replacement & Adjustment Airflow Control System Setup & Calibration Filter Integrity Check Cabinet Leak Tightness Test Main Control Board Description & Replacement Airflow Probe Description & Replacement Section No Error Indicators, Troubleshooting, Diagnostics & Airflow Probe Performance Verification, Option Mode Section No Remote Contacts Section No Optional Equipment Ultraviolet Lamp Section No Electrical/Environmental Requirements Appendix A Films/Pamphlets & Articles Insert List of Agencies Performing Certification Insert Replacement Parts List MANUAL DRAWINGS ACD NU-430 Airflow Schematic BCD NU Specification Drawing BCD NU Specification Drawing BCD Drain Valve Installation BCD Butterfly Valve Installation BCD Front Panel BCD Front Panel (E) BCD Bag In/Bag Out Procedure ASSEMBLY DRAWINGS BCD Base Stand Assembly BCD Base Stand Storage Cabinet Assembly BCD Control Center & Front Decorative Panel Assembly BCD Sliding Window Assembly & Adjustment BCD Base Cabinet Assembly ELECTRICAL SCHEMATICS BCD-05545(Sheets 1-2) NU /600 Electrical Schematic BCD NU E/600E Electrical Schematic BCD NU G/600G Electrical Schematic BCD (Sheets 1-3) I/O Board BCD Control Board Display OM0082 6

7 BCD Airflow Probe Board OM0082 7

8 1.0 General Description Labgard Class II, Type B2 Laminar Flow Biological Safety Cabinet Models NU /600 NU E/600E NU G/600G MANUFACTURED BY: NuAire, Inc. - Plymouth, Minnesota, U.S.A. The LABGARD Model NU-430 Laminar Flow Biological Safety Cabinet (BSC) is a bench/table top model, optionally available with a base support stand, for operation as a console model. The Laminar Flow Biological Safety Cabinet, (BSC) is a product resulting from the development of the "laminar flow" principle and the application of environmental controls as required in the field of biological research or chemical containment. The BSC, when used with proper technique, is an effective laboratory aid in obtaining the optimum control over product quality while reducing the potential for exposure of both product and personnel to airborne biological or particulate chemical agents in low to moderate risk-hazard research and use of the BSC to work with chemicals and trace amounts of radionuclides required as an adjunct to microbiological studies, as prescribed by the Center for Disease Control (CDC) Atlanta, Georgia. The LABGARD bench top/console Model NU-430, when configured with all the available standard options has eleven distinct characteristics that not only enable the cabinet to perform its functions of handling chemicals, radionuclide materials, biologicals, gases, particulate matter and carcinogens, but also enhance its ability to do so in many ways that provide greater reliability and flexibility: ALL WELDED STAINLESS STEEL CONSTRUCTION ENERGY SAVING USE OF OUTSIDE AIR INTEGRAL EXHAUST FILTER HEPA FILTRATION CONTROLLED AIR FLOW SEALED CONSTRUCTION HEPEX PRESSURE PLENUM MINIMUM AIR TURBULENCE BAG-IN/OUT OF EXHAUST HEPA FILTER (Optional) ELECTRONIC AIRFLOW CONTROL SYSTEM ALL FRONT MAINTENANCE 1.1 All Welded Stainless Steel Construction The entire cabinet shell and all internal plenums and structures are constructed from Type 304 stainless steel for increased chemical resistance to acids/solvents which are normally used in applications which require a fume hood. 1.2 Energy Saving Use of Outside Air (Tempered) minimizes the use of room air drawn into the work zone as part of the internal downflow. The NU-430, accepts outside air, prefilters the air (optional), passes it into a blower which pressurizes the air within a plenum from which it flows downward through a HEPA filter into the work zone. The work zone air combines with the room air flowing into the cabinet through the work access opening to flow out of the cabinet through a HEPA filter drawn by the vacuum established by a roof blower. The outside air may come from the aisles or corridors of OM0082 8

9 the building to gain a tempering effect. The maximum extension of the duct is 10 feet without external assistance. OM0082 9

10 1.3 The Integral Exhaust Filter is contained within the cabinet for ease of maintenance and minimum initial installation cost. The exhaust fan is usually on the roof which places the entire cabinet airflow system at a vacuum relative into the room and building. This system provides the safest possible control - particulate contaminants are removed from the cabinet and ducts in the shortest possible flow path; the vacuum flow path precludes the change of contaminant leakage into the building. The contaminated air flow from the work zone through the cabinet is contained in all metal ducts free from any electrical components. 1.4 High Efficiency Particulate Air (HEPA) filtration is utilized for both supply and exhaust air systems. The HEPA filters are rated at 99.99% efficiency on removal of all particulate matter 0.3 micron. Efficiency improves on both smaller and larger particles. NuAire's HEPEX Zero Leak Airflow system is used for the supply HEPA filter. 1.5 Controlled air flow establishes an optimum air balance between the cabinet's laminar airflow system and the exhaust airflow system. Work zone air downflow is uniform and held to an average velocity of 60 ± 5 LFPM (.30 ±.025 m/s) measured in the plane of the bottom edge of the viewing window. The significance of this airflow control is that the work zone air is flowing down like a huge piston carrying contaminants rapidly out of the work zone with 24 air changes per minute. The work zone airflow is ducted such that the quantity of air leaving through the rear perforated area is half of the downflow air quantity. The remainder, that is the other half of the downflow air, and the air entering through the work access opening flow into the front perforated area. Room air flowing into the 8 inch (203mm) work access opening forms the air barrier protecting personnel at 105 ± 5 fpm (.53 ±.025 m/s). 1.6 Sealed Construction requires that the entire cabinet shell and all air ducts and plenums be leak-tight. Penetrations necessary for utilities, access panel doors, etc., are sealed in such a manner or maintained under vacuum as to prevent escape of potentially contaminated air into the room, even when not-operating except the work access opening. 1.7 HEPEX Pressure Plenum by NuAire provides greater reliability for a continuing Class 100, Federal Standard 209d, Bio-clean environment within the work zone of the cabinet. The air-space between the HEPEX and the cabinet structure is always less than room ambient pressure which prevents any air outflow from the cabinet other than the filtered outflow from the exhaust filter. The absolute filter is nested in non-flammable elastomeric foam in a manner which insures zero leak and preserves the Class 100 environment free from rigors of shipping and moving. 1.8 Minimum Air Turbulence is maintained by having all work zone enclosure walls (including the viewing window) provide a fixed flow area perpendicular to the air flow streamlines. This condition of minimum turbulence with no upward or refluxing component is optimized by having each enclosure wall essentially become an extension of the true flow area of the HEPA filter - thus permitting the clean filtered air to flow downward like a huge piston forcing clean air onto the work tray before the air recirculates through the cabinet airflow/blower system. OM

11 Minimizing turbulence at the front opening is effected by the airfoil turning the air stream entering the cabinet to a direction parallel to the flow of the recirculated air within the unit thus preventing the recirculated air from flowing out through the front opening into the room and the room air entering the cabinet from overflowing onto the work surface. Truncating the inlet grill against the sidewall of the cabinet produces air velocities 25% greater around the sidewall airfoil, protecting this critical area from migrating particles. In addition, the large volume of air provided by the access opening at 100 LFPM produces more energy to escape. Escape velocities are directed proportional to the volume, and inversely proportional to the area plus the square of distance from the inlet grill. 1.9 The Bag IN/OUT of EXHAUST HEPA FILTER (optional) maintains a barrier between the operator and dangerous material. The contaminated filter can be removed from the area and a new filter installed without exposing maintenance personnel. In the event that any kind of an accident occurs such as having a used filter dropped during maintenance, the Bag IN/OUT technique and the exhaust filter integral with the cabinet provide both primary (the Bag) and secondary containment (the room) against breach of the pressure boundary by cordoning off the room as a contaminated zone. Having the exhaust filter in a remote area could permit an accident to escalate Electronic Airflow Control System is a self contained microprocessor driven module that monitors and controls all cabinet functions. A dual thermister airflow monitor probe is located in the workzone downflow air monitoring and controlling to an established calibrated setpoint. The exhaust flow is also monitored and displayed as total flow with a dual thermistor airflow probe. The exhaust control system is interlocked to the internal blower and will not permit it to energize unless the exhaust flow is sufficient to provide a minimum inflow average horizontal vector of approximately 100 LFPM (.51 m/s) air barrier. Having achieved internal blower operation with the correct exhaust flow, the internal blower will remain operational with decreasing exhaust flow until the air barrier inflow velocity drops to lower alarm limit at which time the internal blower cuts out; the air barrier inflow velocity surges immediately providing an immediate short interval purge of the workzone Front Maintenance permits the BSC to be permanently connected to a plant exhaust system and plumbing lines. Both supply (downflow) and exhaust HEPA filters are rapidly and easily removed from the front of the cabinet. All controls and adjustments are accessible from the front of the cabinet. OM

12 NU-430 Airflow Schematic (ACD-05651) (REV A) OM

13 2.0 Construction Features Standard Features 2.1 The NU-430 cabinet shell is pressure tight, consisting of all welded, 16 gauge, type 304 stainless steel wrap. All welds are meticulously hand-finished and polished to be free of burrs. The wrap is structurally reinforced to minimize vibration and maintain surface flatness. The stainless steel is finished in easily cleanable No. 4 satin. The cold-rolled steel 14 gauge access panel and light/control center is finished in textured baked urethane. Colors available upon request. 2.2 All LABGARD controls are contained within an easily removable control center, placed at eye level for convenience. All connections from the control center to the cabinet are hermetically sealed at the cabinet shell, with quick disconnect electrical connections at the control center. The control center is hinged for easy access to electrical components and the fluorescent light. 2.3 All cabinet surfaces, interior and exterior, are constructed of, or furnished with, materials which are corrosion, flame and moisture resistant and which will not deteriorate under exposure to liquid or vapor phase decontaminates such as hydrogen peroxide, formaldehyde, alcohol, iodophers, peracetic acid. USE OF CHLORINATED OR HALOGEN MATERIALS MAY DAMAGE STAINLESS STEEL. 2.4 The work area consists of the following items going from front to back: the airfoil, the work access opening, the removable perforated stainless steel inlet grill, the removable solid stainless steel work tray and the perforated stainless steel air exhaust area located in the rear wall of the work area. The top lip of the airfoil is one inch above the removable solid stainless steel work tray, which extends a full length and width of the workspace. 2.5 Air leaves the workspace via the perforated grill in front of the work tray, and via a similar perforated grill extending the full length of the workspace in the rear wall just above the work tray. The air removed via the front and rear perforated grills is exhausted through a HEPA filter located in the top right side of the cabinet. 2.6 This cabinet FLOW-THRU construction insures that air contaminated with chemicals, particulates, radioactive materials, etc. flow only through internal cabinet stainless steel ducts directly to the roof fan, through customer installed duct work. This design prevents contaminants generated within work zone from contact with the supply blower or with any electrical equipment. Note that the supply and exhaust HEPA filters are in series with each other. 2.7 The supply filter is of full work zone depth and width and is protected with an easily removable, perforated metal diffuser, in order to check filter integrity. 2.8 Both the supply filter and exhaust HEPA filters are 99.99% efficient on particulate 0.3 microns in diameter with even greater efficiency on larger and/or smaller particles. When replacing the HEPA filters, use only filters of the same size and flow rating as originally installed (see Replacement Parts List). OM

14 2.9 A drain-spillage plenum is located below the work surface, so arranged as to catch any spillage in the work area, and is easily cleaned after the removal of the solid work tray. A 3/8 inch (10mm) ball-type drain valve is located on the right front of the plenum to handle large spills. The drain spillage trough accommodates a minimum of five (5) liters. This area also forms the air path to the exhaust plenum The viewing window is a full counter balanced sliding 1/4-inch (6mm) tempered glass window that provides for smooth opening/closing. The window provides an 18 1/2-inch (470mm) maximum open area to fully closed. The window frame is constructed from type 304 stainless steel with all exposed edges deburred for easy cleaning, finished in No. 4 satin grain. Both audible and visual alarms are provided when the window is raised above the standard work opening height, 8 inches (203mm) The two (T8) fluorescent lamps are cool white, rapid start type, located outside the work space, obscured from direct view providing an average uniform light intensity of 1000 foot candles (1076 LUX) at the work surface. The electronic ballast contains thermal protection with automatic reset. PLEASE NOTE, ELECTRONIC BALLASTS OPERATE WITH HIGH INRUSH CURRENT. IT IS NOT RECOMMENDED TO USE THIS PRODUCT WITH GROUND FAULT CIRCUIT INTERRUPTERS (GFCI'S) BECAUSE THE BALLASTS MAY CAUSE THE GFCI TO TRIP Electrical power is required from one 115 or 230 Vac, 50 or 60 Hz, 20 Amp power source. The power is distributed internally for the blower, lights and duplex outlets via solid state relays and triacs. Cabinet shell and all electrical components are grounded back to electrical source to meet grounding continuity requirements for electrical safety The motor/blower requires 115 or 230 Volt, single phase, 50 or 60 Hz power and will not exceed a temperature of 105 C in a maximum ambient temperature of 48 C (120 F) under any maximum load condition (class "B" insulation). The thermal protector will not trip at 115% of the rated voltage under maximum load and ambient temperature conditions. The motor/blower is rated for 24-hour continuous operation and is lubricated for life The LABGARD has only one motor/blower fan system controlled by the Electronic Airflow Control System for the downflow air. The motor/blower and blades are fabricated of or protected with corrosion resistant materials to withstand normal laboratory or chemical fumes. The motor/blower will automatically compensate for airflow as the filters load with particulate to achieve a fan delivery fall off of no more than 10% as a result of a 80% increase in pressure drop across the filters. The Electronic Airflow Control System will provide a greater than 200% increase in pressure drop across the filters is achievable The LABGARD is designed for cleanability: surface decontamination is made simple by use of coved corners and an easily removable work tray over the spillage trough. Complete biological decontamination can be accomplished by following the recommended NIH procedure using formaldehyde. The NIH procedure can be obtained from the National Audiovisual Center (GS) Washington, D.C., in the form of a tape cassette and slides A duplex electrical outlet is located outside the workspace in the left vertical airfoil as standard. The outlet is rated at line voltage 3 Amp maximum. Outlets may be placed OM

15 within the workzone if appropriate warning labels are provided, to alert the operator that chemical contamination may cause explosion. OM

16 2.17 Two service valves for gas service are located in the right side wall as standard. The valve body and handle is made from chrome plated brass forgings with the valve ground and lapped and tested at 100 psi (7.0 Bar). Service valves have integral ten serration outlet and are individually coded by a removable button on the handle. Service valves are rated 30 psi (2.0 Bar) working pressure Both supply and exhaust cabinet connections are 12-inch (305mm) diameter Normally plugged D.O.P. challenge ports are provided on the top of the cabinet. The ports provide access to the supply and exhaust pressure plenums for checking the challange concentration when leak testing the HEPA filters, if the measuring instrument requires such access The NU-430 incorporating the use of the electronic airflow control system offers improved cabinet performance. The control system uses a dual thermister airflow probe in the downflow stream to monitor and control airflow to setpoints. The control system automatically compensates for filter loading, voltage variances and other environmental effects. A second dual thermistor airflow probe in the exhaust airstream monitors for exhaust volume. Both downflow and exhaust are displayed on the front panel. The control system also monitors the sliding window position with a microswitch for both window height and window closed positions. The control system through the use of the front panel, controls the on/off function of the fluorescent and ultraviolet lights (optional), outlets and blower. The control system also allows contact closure outputs for interaction with HVAC systems to optimize environmental performance An Exhaust Interlock System prevents operation of the internal supply blower unless the exhaust flow is sufficient to provide a minimum inflow velocity horizontal vector of 100 FPM through the work access opening, corresponding to the total flow. Note: The blower switch will turn the green pilot light on (green pilot light will blink until internal blower turns on), but the internal blower will not run until the exhaust flow provides the 100 FPM as discussed in the foregoing paragraph The textured baked urethane finish is a high-solids chemical agent resistant coating (CARC), and meets new United States Environmental Protection Agency (EPA) solvent emission regulations with less than 3.5 lb./gal. (.419Kg/Lit) volatile organic content. The CARC coating contains no chlorinated hydrocarbons and withstand chemical and biological reagents absorption into the paint. They survive corrosive, humid and salt environments. Optional Features 2.23 An ultraviolet light provides a minimum of 40 micro-watts per square centimeter on the work tray, controlled from the Control Center with a switch which is controlled so that the UV light cannot be used when the sliding window is opened or the normal fluorescent lamps are on (see Section 12.1) Ground-key cocks (up to three) can be installed in either or both side walls. The type of service is indicated by a removable button on the handle. Needle valves for highpressure gases are optionally available. OM

17 2.25 Remote controlled needle valves can be added to replace and/or in addition to the service valves. Up to three can be installed on either or both side airfoils of the cabinet. The type of service is indicated by a replaceable button on the handle. Alternatively the handles and hose barb outlet may be color coated and/or a chemically resistive coating can be supplied The base support stand is constructed from 16 gauge, all welded 2-inch (51mm) square steel tubing, finished in textured baked urethane. The base stand heights are 26 1/2-inches or 32 1/2-inches (673mm or 825mm) which provide work surface heights of 30-inches or 36-inches (762mm or 914mm) respectively. The base support stand is available as a base storage cabinet, that is fabricated from 16-gauge cold-rolled steel. It consists of rear, end and bottom panels fastened to the base stand via screws. Sliding doors provide complete access to the 16-inch (406mm) cabinets interior height (depth is 22-inches) (559mm). NSF approved leg levelers permit ± 3/4-inch (20mm) of height adjustment. The base support stand is shipped knocked-down for on-site assembly A ground fault circuit interrupter, (GFI) can be purchased to replace the standard duplex outlet on the airfoil (115 Vac only) Bag-in/Bag-out of the exhaust HEPA filter protects the maintenance technician from being exposed to contaminants while changing the filter. The filter clamp mechanism is located external to the cabinet and uses a 5/16-inch (8 mm) end wrench The cabinet can be configured with plastic storage trays, which are attached to the underside of the cabinet via stainless steel rails. The trays are high-impact polyethylene plastic available in red, brown, blue or yellow. The base stand is a prerequisite for the trays Decorative side panels can be added to cover plumbing lines to the services within the interior. The panels are constructed from 16 gauge, cold rolled steel finished in textured baked urethane A prefilter can be added to the supply air inlet. The prefilter frame is constructed from 16 gauge cold-rolled steel and finished in textured baked urethane. Prefilter service is accessible from the front The sliding view screen can be replaced by a hinged window. The window is 1/4-inch thick tempered glass sealed in a heavy rubber gasket to a stainless steel window frame. Cam locks hold the window in place when closed. Hydraulic lifts are provided to assist window opening and cleaning and holds the window at a 30 degree angle from horizontal In order to adjust the exhaust flow, NuAire offers a gas-tight manual butterfly valve, Model NU-940. The valve is constructed of 14 gauge, 12 inch (305mm) round stainless steel, with a stainless steel gasketed valve that is pressure tested at 2.0 inches (.51mm) water gauge. In conjunction with an electronic airflow control system. NuAire offers a damper actuated butterfly valve, Model NU-951. The two work in concert to provide complete airflow control through the cabinet to: 1. Compensate for exhaust HEPA filter loading 2. Compensate for exhaust static pressure flow changes 3. Permit multi-ganging of several cabinets 4. Provide for night-setback energy savings. OM

18 2.34 A sorbent filter module may be added to the top of the exhaust HEPA filter chamber to treat the exhaust efflux. The chamber is constructed from 16 gauge cold-rolled steel, painted in textured baked urethane and provides front access filter removal. The chamber houses 2 each 1-3/4 inch thick sorbent filter beds, that provides a residense time of approximately 0.1 seconds. The sorbent filter may be impregnated to remove specific contaminants NuAire offers various airflow control options to enhance and optimize performance characteristics of the cabinet and its environment. Night setback switch - The night setback switch is used to initiate the control system into the night setback mode. The night setback mode is used to reduce the exhaust air volume during non-usage periods resulting in conditioned air energy savings. For the night setback mode to operate, the NU motorized air-tight butterfly valve must be installed to provide the means for reduction of exhaust airflow. Exhaust airflow loss override - The exhaust airflow loss override is used with the NU motorized air-tight butterfly valve. A pressure switch is used to monitor the exhaust system's static pressure. If static pressure is not present (i.e. exhaust system failure), the pressure switch will close the remote override contacts on the main control board and send a signal to close the butterfly valve. When static pressure is present, the butterfly valve will open and control the exhaust volume to setpoint. Cabinet off override - The cabinet off override is used with the NU motorized air-tight butterfly valve. The blower on/off switch, when turned off, will close the remote override contacts on the main control board and send a signal to close the butterfly valve. When the blower switch is turned on, the butterfly valve will open and control the exhaust volume to setpoint. Supply air on/off control - The supply air on/off control is used with the NU motorized air-tight butterfly valve. The butterfly valve is controlled in conjunction with the blower on/off switch on the control panel. When the blower switch is on, the butterfly valve will open. When the blower switch is off, the butterfly will close. Delay on supply airflow - The delay on supply airflow will delay the blower, from turning on for 60 seconds once the blower "on" conditions are met. The blower "on" has two conditions, first, the blower on switch is on and second, there is sufficient exhaust volume. Once these conditions are met, the delay count is initiated and lasts for 60 seconds. Upon expiration of the 60 second count, the blower will turn on. Delay off exhaust airflow - If the exhaust system is to be interfaced to the cabinet control system. The blower on/off switch will then send a signal to the exhaust system to turn on/off. A 60 second delay can be added when turning off the blower to assure the cabinet remains under negative pressure throughout the on/off sequence. OM

19 3.0 Models & Features The model NU-430, Class II, Type B2 Laminar Flow Biological Safety Cabinet is manufactured in two sizes: 4 ft. and 6 ft. (1.2m and 1.8m). 3.1 Dimensions NU (E)(G) NU (E)(G) Overall Dimensions - Inches (mm): Width (W) 53 5/8 (1362) 77 5/8 (1972) Depth (D) (includes Control Center): 32 7/8 (835) 32 7/8 (835) Height (H) (includes Exhaust Collar): 61 1/2 (1562) 61 1/2 (1562) Base Stand, 30" (762mm) w.s.: 88 (2235) 88 (2235) Base Stand, 36" (914mm) w.s.: 94 (2388) 94 (2388) Interior Dimensions: Width (W): 46 3/8 (1178) 70 3/8 (1788) Depth (D): 23 1/2 (597) 23 1/2 (597) Height (H): 25 1/2 (648) 25 1/2 (648) 3.2 Standard Features Electronic Airflow Control System HEPEX Zero Leak Airflow System Large HEPA Filters; 99.99% Efficient on 0.3 Microns External Fluorescent Lighting: 100 Ft. Candles (1076 LUX) Front Filter Removal Sliding Glass View Screen; 18 1/2" (470mm) Opening to Fully Closing Standard 8 Inch (203mm) Access Opening Removable Work Tray Metal Diffuser Over Supply HEPA Tempered Glass Window One Outlet (Left Front Fairing) Two Gas Service Valves (Right Side Wall) Spill Trough with 3/8" (10mm) Drain Valve Exhaust Air Plenum Paper Catch Single Point Exhaust HEPA Filter Release OM

20 3.3 Optional Features Bag In/Bag Out of Exhaust HEPA Filter with Single Point External Filter Release Ultraviolet Light Additional Service Valves for Gas, Air, Vacuum Remote Service Valves Additional Duplex Outlet Ground Fault Interrupter for Electrical System IV Bar with 6 Stainless Steel Hooks Gas Tight Butterfly Valves (Manual or Automatic) Base Support Stand (available in standard working surface heights of 30 or 36 inches) (762 or 914mm) With or Without Storage Shelves Adjustable Control for Support Stand or Storage Cabinet Hinged Viewing Window Microscope Viewing Window Sinks with Hot/Cold or DI Water Faucets Storage Pull-Out Trays Sorbent Exhaust Filter Module Decorative Side Panels (hides plumbing fixture connections) Prefilter for Supply Air Metal Framed HEPA Filters HEPA Filters 0.3 Micron Arm Rest Roof Exhaust Blower Modules OM

21 (BCD-05412) (REV G) OM

22 (BCD-05413) (REV D) OM

23 4.0 Test Performance & Procedures All equipment is thoroughly inspected at the NuAire Factory at the time of shipment. Quality control is maintained by constant surveillance over the product, beginning at the receipt of purchased material and concluding with a final inspection which certifies cabinet performance to the National Sanitation Foundation Standard No. 49, as well as any unique customer requirements. In all instances where product quality cannot be easily assessed on the end item, product inspection is performed during sub-assembly fabrication. The following test procedures are conducted on each cabinet and a copy of the test report is included with each unit. 4.1 Personnel, Product & Cross-Contamination Tests (See Note) NSF Std. No. 49 and defines testing procedures using an aerosolized spore solution to challenge the containment properties of the cabinet Personnel Protection Test The spores are aerosolized via a nebulizer placed 14" (356mm) above the work surface, 4" behind the view screen, directed at the view screen. The challenge is 1 x 10 8 spores per milliliter solution. Six air samplers (AGI's) are placed above and below the work access opening centered around a cylinder, simulating an arm and two slit air samplers are placed 8" (203mm) from each end of the work access opening. No more than 10 CFUs* can be recovered from the AGI's and 5 from the slit sampler for a thirty minute test. Total of 3 tests Product Protection Test The entire work surface is covered with open agar settling plates. The nebulizer is placed 4" (102mm) inches from the bottom outside edge of the view screen directed at the view screen. A cylinder is placed directly below the nebulizer 2" (50mm) above the work tray simulating an arm. No more than 5 CFUs* can be recovered for a 5 minute test. Total of 3 tests Cross Contamination Test The entire work surface is covered with open agar settling plates. The nebulizer is placed 3 to 5 inches (76-127mm) above the work surface, against a side wall, directed toward the other side wall. No more than 2 CFUs* can be recovered at a distance greater than 14 inches (356mm) from the challenged side wall. Both side walls are challenged 3 times. 4.2 Leak Tightness Test The cabinet work zone opening as well as the exhaust HEPA filter area are sealed with a plate clamped into place. The cabinet is pressurized, to 2 inches of water gage (50 mm) using air. All welds, joints, access door/panels and penetrations in the cabinet structure are probed for leaks using soap solution. The cabinet must hold 2 inches water gage (50 mm) pressure, with no detectable leaks, or hold pressure within 10% for 30 minutes. *CFU = Colony Forming Unit OM

24 4.3 HEPA Filter Integrity/Housing & Frame Leak Test Both supply and exhaust HEPA filters are scanned for leaks around the filter seal periphery, filter housing and filter media. Passes are made overlapping to ensure complete coverage. D.O.P. smoke is introduced upstream using one Laskin Nozzle per each 135 CFM (752 m 3 /H). Downstream measurements using an Aerosol Linear Photometer must be less than 0.01% of upstream concentration. 4.4 Downflow Velocity Measurement NuAire establishes an average downflow air velocity of 60 LFPM ± 5 LFPM (.30 m/s ±.025 m/s). Measurements are taken on an approximate 6 inch (152mm) grid using an Alnor 8500 Thermoanemometer in a horizontal plane defined by the bottom edge of the viewing window. All measurements fall between ± 20 percent of the stated average velocity. Average velocity will fall between LFPM ( m/s). 4.5 Smoke Flow Patterns Smoke is passed 1-1/2 inches (38mm) in front of the entire perimeter of the work access opening; all smoke movement shall be inward. Airflow within the work area shall be downward with no dead spots, refluxing or drift. 4.6 Inflow Air Velocity Measurement (Direct Reading Instrument or Inflow Velocity Measurement) Inflow air velocity must be calculated using the following equation: Inflow Air Velocity = Total Flow Volume - Downflow Volume Work Access Opening Area Measure total flow volume through the work access opening using a direct reading instrument (shortridge flowhood ADM-870) or measured on a 4-inch (102mm) grid in two rows at 25% and 75% of the access opening height using an Alnor 8500 or TSI 8355 Thermoanemometer. If using a grid measurement, the front grill should be removed and the work tray inverted and placed at the front of the workzone to produce a more laminar airflow through the work access opening. Also, during the above procedures, the internal blower should be turned off and the inlet duct connection blocked off to assure all airflow is drawn through the work access opening. The inflow air volume quantity is sufficient to provide a calculated velocity of 105, ± 5 LFPM (.53 ±.025 m/s) through the work access opening. 4.7 Temperature Rise The cabinet, operated with lights on, exhibits no more than 8.5 C (15 F) temperature rise over ambient after 4 hours of operation. 4.8 Lighting Intensity Lighting Intensity, measured on a 6 inch (152mm) grid, with an average background of foot-candles ( LUX), average between 90 (968) to 120 (1291) foot-candles (LUX) on the work tray. Individual readings are not below 60 (646), nor above 175 (1883) foot-candles (LUX), on the work tray as measured by a DLM2 light meter. 4.9 Noise Level Overall noise measured 12 inches (305mm) in front of the work area opening and at a level 15 inches (381mm) above the work tray will not exceed 67 dba with a background not over 55 dba using a General Radio model 1982 or Quest Model 1800 Sound Level Meter. OM

25 4.10 Vibration Vibration amplitude will not exceed 200 micro inches RMS is measured with a Bruel and Kjaer, type 2511 in the center of the work tray in three principle plane (no dimensional requirements) at 20 to 20,000 hertz Electrical Leakage Test A.C. electrical leakage shall not exceed 500 micro amperes using a Simpson Model 229 or equivalent tester (700 microamperes for 230 Vac units). Note, this cabinet contains primary circuit filtering and may exceed 500/700 microamperes, but not more than 3.5 milliamperes Dielectric Voltage - Withstand 1770 (VDC ONLY) is applied between dead metal parts and the hot/neutral power source lead with no electrical breakdown using a Associated Research Model 4045AI (2100 (VDC ONLY) for 230 Vac units) Grounding Continuity The resistance between the green bonding conductor of supply cord and any dead metal part of the cabinet shall not exceed 0.10 ohms. Note: NuAire has conducted the test on a representative production cabinet in order to validate the design and containment properties of the design. NuAire will periodically conduct these tests as a Quality Assurance measure. Cabinets so tested are labeled and decontaminated prior to shipment. These tests may also be conducted at customer request and expense. All of the other above tests are conducted on each cabinet prior to shipment and a copy of the test report accompanies this Operation and Maintenance Manual. OM

26 5.0 Warranty NuAire, Inc. warrants that it will repair F.O.B. its factory or furnish without charge F.O.B. its factory a similar part to replace any material in its equipment within 36 months after the date of sale if proved to the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or accessories are warranted only to the extent guaranteed by the original manufacturer, and this warranty shall not apply to any portion of the equipment modified by the user. Claims under this warranty should be directed to NuAire, Inc. setting forth in detail the nature of the defect, the date of the initial installation and the serial and model number of the equipment. This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse, abuse, accident, shipping damage, improper installation or service, or damage by fire, flood or acts of God. If the serial number of this product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety. The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any item, for any reason, contact NuAire for a Return Authorization Number. This number must accompany all returns. Any product shipped to NuAire without this number will be returned refused shipment or collect freight. 6.0 Shipments NuAire takes every reasonable precaution to assure that your LABGARD cabinet arrives without damage. Motor carriers are carefully selected and shipping cartons have been specially designed to insure your purchase. However, damage can occur in any shipment and the following outlines the steps you should take on receipt of a NuAire LABGARD cabinet to be sure that if damage has occurred, the proper claims and actions are taken immediately. 6.1 Damaged Shipments Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the basis of your filing the claim against the carrier If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and request an inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain date and that he failed to comply with the request. This along with other papers in the customer's possession will support the claim. OM

27 7.0 Installation Instructions 7.1 Location Within the laboratory, pharmacy, etc., the ideal location of the biological safety cabinet is away from personnel traffic lanes, air vents (in or out), doors and/or any other source of disruptive air currents. If drafts or other disruptive air currents exceed the inflow velocity of the cabinet through the access opening, the potential exists for contaminated air to exit or enter the work zone area of the cabinet. It depends on the severity of the air current. REMEMBER: A BIOLOGICAL SAFETY CABINET IS NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE. Where space permits, a clear 12" (305mm) area should be permitted on each side of the cabinet for maintenance purposes. The electrical outlet into which the cabinet is connected should be readily accessible for maintenance purposes. If the outlet is inaccessible, such as a conduit (hardwired) connection, then an appropriate warning label should be applied near the cabinet's on/off switch, to indicate the circuit breaker on the power distribution panel to be used. More than any other biological safety cabinet, the NU-430 requires careful site-planning and preparation, due to the total exhaust nature of the cabinet. Proper sizing of the exhaust and make up supply systems are critical to the successful installation of the cabinet. In addition, the cabinet provides for the choice of make-up air for the supply (downflow) air. The following are airflow requirements. Air Volume (CFM/CMH) Supply Air Inflow Exhaust Air* NU (E)(G) 484/ / /1281 NU (E)(G) 690/ / /1869 *Exhaust air volume at 2.0 inches (50.8mm) w.g. negative. NuAire offers a complete selection of roof exhaust modules which have been specifically designed to support the installation of the NU-430. The module selected is dependent on the length and size of the ductwork needed to connect the cabinet to the exhaust blower. Design guidelines are discussed in a later section. 7.2 Set-Up Instructions Remove outer shipping protection (carton or crating). The cabinet is fastened to the base skid and it is usually the best procedure to leave the skid in place until the cabinet is located in its approximate position to facilitate ease in handling. It can then be removed from the skid by removing the banding holding the cabinet to the skid. It may be necessary to remove the Control Center in order to gain passage through a doorway. It may easily be removed by following the instructions on drawing BCD OM

28 7.2.1 Base Stand Assembly The base stand is shipped knocked down in a separate carton and is assembled per drawing BCD if accompanied with the unit. Remove the banding holding the cabinet to the base skid. Lift the cabinet from the base skid and place on the floor. Now lift the cabinet on top of the base and bolt the base stand to the cabinet using two 3/8" - 16 x 3/4" bolts and washers provided for the front base stand tabs and two 1/4" acorn nuts for the rear weld studs. Place the cabinet in its desired location. The base stand storage cabinets will usually be shipped according to customer requirements. If it is shipped unassembled, it can be assembled per drawing BCD It is recommended that the upper and lower base stand braces be installed first, then the rear and bottom panels (the end panels are always prefastened). Once assembled, fasten the cabinet per the above instructions. Remove the cap protecting the drain valve threads and install the drain valve, on the bottom right front of the cabinet using Loctite 242 furnished to the threads and rotate the valve body until it is secure (see BCD-05154) Leveling Using a level placed on the work tray, adjust the leg levelers, first, end to end, then, front to back. The NSF approved leg levelers provide a ± 3/4" (20mm) adjustment Bench Installation (BCD-05154) Place the cabinet on the bench with approximately a 2" (50mm) overhang clearance for installation of the drain valve. If the drain valve is not desired, place the cabinet in its desired location and using RTV caulk, seal all around the base of the cabinet and the bench. This provides a tight seal to prevent bench spills from migrating under the cabinet. If a drain valve is desired, remove the handle from the valve stem to gain clearance for valve body rotation. Add Loctite 242 (furnished) to the threads and rotate valve body until secure, with the valve stem (for handle) on the left side. Re-install handle to valve stem. Adjust the cabinet on bench to provide a 1-1/2" (38mm) overhang and seal the interface of the bench and cabinet, using RTV caulk as above Plumbing Services Service valves with the type of service specified by the removable button on the handle, are located in the work zone. The service valves are not recommended for pressure over 30 p.s.i. (2.0 BAR). Reducing valves should be installed external to the cabinet if necessary. Service valves should never be used for oxygen service. A special needle valve for oxygen service is required and available upon request. OM

29 External connection is to 3/8 inch NPT coupling in the inner side walls. Connection to plant utilities should be made with proper materials for the individual service and according to national and/or local codes. It is not recommended that flammable gases be used in the cabinet. However, if flammable gas is used, emergency shut-off valves should be located in an accessible area external to the cabinet. Observe all labels pertaining to the type of service and operating pressure. THIS UNIT HAS NOT BEEN EVALUATED FOR USE WITH FLAMMABLE, TOXIC OR EXPLOSIVE SUBSTANCES. USE AT YOUR OWN RISK. Remote controlled needle-valve plumbing fixtures can be optionally provided within the interior sidewalls. Control handles are located externally on the vertical airfoil. Service outlets within the interior have serrated tapered fittings designed for hose connections with the remote controlled needle valve plumbing fixtures. NuAire provides for either rear, bottom or top connections of plumbing services to plant utilities. Connection from the needle valve assembly to the welded exit coupling is accomplished with the supplied 3/8 inch soft copper tubing as standard (alternative materials to meet local codes are available upon request). The needle valves are not recommended for working pressure in excess of 100 p.s.i. (7.0 BAR). OM

30 (BCD-05154) (REV B) OM

31 7.2.5 Electrical Services The NU-430 series Biological Safety Cabinets may be "hardwired" (optional) or connected via an electrical power cord which is standard. The unit requires 20 Amps single phase. It is recommended that power to the unit be on its own branch circuit, protected with a 20 Amp circuit breaker respectively or fuse at the distribution panel. PLEASE NOTE, THIS UNIT CONTAINS ELECTRONIC BALLASTS FOR THE FLUORESCENT LIGHTING. ELECTRONIC BALLASTS OPERATE WITH HIGH INRUSH CURRENT. IT IS NOT RECOMMENDED TO USE THIS PRODUCT WITH GROUND FAULT CIRCUIT INTERRUPTERS (GFCI'S) BECAUSE THE BALLASTS MAY CAUSE THE GFCI TO TRIP Exhaust/Supply Duct Installation Guidelines The exhaust/supply systems must provide conditions similar to that under which the cabinet was certified to meet its stated performance. The following guidelines should be observed when installing exhaust/supply air ductwork for either existing plant exhaust systems, or a new exhaust system. 1. Adequate room make-up air inflow to replace exhausted air. Air diffusion rate not to exceed velocity of 100 LFPM (.51 m/s) to minimize disruptive air currents. Make-Up Air Requirements (CFM/CMH) Model With Supply Duct Without Supply Duct NU (E)(G) 454/ /1230 NU (E)(G) 690/ / Adequate plant exhaust system capability. The exhaust system is usually adequate if it can provide the rated exhaust flow at 2.0 inches (50.8mm) water gauge. 3. Adequate supply air capability (if used). The supply air system is usually adequate if it can provide the rated supply air at 0.0 inches water gauge positive. 4. The supply air must be interlocked with the operation of the cabinet's internal downflow blower in order to prevent downflow air from being forced out of the front of the cabinet when powered off or during nite set-back conditions. NuAire provides fan relay contacts for this purpose. 5. All duct losses must be considered and added to the cabinet loss in selecting the exhaust blower, for a new exhaust system (i.e. duct diameter, length and number of elbows, etc.). 6. All duct work should be securely anchored to the building construction in manner to be free from vibration and swaying under all conditions of operations. 7. Sheet metal gauges and seams should be in accordance with the current edition of the ASHRAE guide. A minimum of 20 gauge for round duct is required to prevent duct collapse due to high static pressure conditions (square duct will require heavier gauge material). OM

32 8. All duct work should be maintained at a negative pressure within the building (i.e. externally located exhaust blower). 9. The exhaust blower and duct work should be a sealed system that can hold 2.0 inches w.g. pressure for 30 minutes with no more than a 10% drop in pressure, and be properly vented to the atmosphere to disperse exhausted air. 10. A local exhaust flow damper should be readily accessible for the maintenance technician/certifier to adjust the total flow through the cabinet. Dampers should be installed with a locking quadrant with markings to indicate damper position. NuAire Model NU-940 manual butterfly or NU-951 automatic butterfly for full automated volume air control are suitable for this purpose. 11. It is recommended that the cabinet operation be interlocked with the exhaust blower. Fan relay contacts are provided for this purpose. However, it is also recommended to have a manual exhaust override switch near the cabinet for certification and service. For multi-ganged systems, this switch could be used to interface with a building automated airflow control system. NuAire also provides several different optional interlocks that will sequence the operation of the cabinet and the exhaust blower. 12. It is recommended that when using the NU automatic butterfly valve, the system air volume must remain within ten percent of the given nominal setpoint volume. Variations greater than ten percent will result in incorrect air volume measurements on the electronic airflow control system. 13. It is not recommended to hard connect (i.e. weld) the exhaust connection to the cabinet. This may damage the exhaust filter and/or the butterfly valve (if present). A neoprene sleeve (NuAire Part No. NU ), banded between the cabinet's exhaust duct and the plant exhaust duct is recommended, with no more than a two inch gap between the ducts, for a 1/8 inch (3mm) thick neoprene sleeve. OM

33 (BCD-05572) (REV C) OM

34 7.2.7 Final Assembly Remove the protective cardboard cover over the supply and exhaust connections on top of the cabinet. Attach the exhaust sensor shroud over the exhaust sensor. Access is provided either through the 12-inch (305mm) exhaust connection or the filter/sensor access panel. The shroud should be placed as close as possible to the exhaust HEPA filter without coming in contact. The probe gasket should be tightly against the sensor shroud to prevent sneak airflow paths. The exterior surface and viewing glass are easily cleaned with any mild household detergent cleaner using a soft cloth. Harsh chemicals, solvent-type cleaners and abrasive cleaners should not be used. Do not attempt to clean the HEPA filter media. Cabinet interior walls or work surface are easily cleaned with any mild household detergent cleaner using a soft cloth. Turn the cabinet on and let it operate for 60 minutes before using it as a LFBSC. 7.3 Exhaust/Supply Air Checks NOTE: THE INTERNAL SUPPLY BLOWER IS INTERLOCKED WITH THE EXHAUST SENSOR, TO PREVENT OPERATION UNLESS ADEQUATE EXHAUST FLOW IS PRESENT Exhaust Volume The exhaust volume is displayed on the front panel. Preset lower and upper alarm limits are factory set but can be field verified at any time. To insure that adequate exhaust is available for a dirty exhaust HEPA filter, condition, the nominal exhaust readings should be attainable with the butterfly valve or damper set at 60 percent open, with all other dampers in the system (duct) open. OM

35 7.3.2 Supply Volume The supply volume is controlled by the electronic airflow control system. The control system uses a dual thermister airflow probe in the downflow air stream to monitor and control airflow to setpoint. The control system automatically compensates for filter loading, voltage variances and other environmental effects. However, excessive variances caused by remote butterfly valves or dampers, insufficient supply air and dirty prefilters may cause an airflow alarm condition to occur. NOTE: THE SUPPLY AIR, IF INTEGRALLY DESIGNED, MUST BE INTERLOCKED WITH THE INTERNAL FAN RELAY TO ASSURE PROPER OPERATION. 7.4 Certification After installation and prior to use, NuAire recommends that the cabinet be certified or commissioned to factory standards. At a minimum, the following tests should be performed (see Section 4.0). 1. HEPA filter media 2. Filter frame leak test 3. Airflow velocities 4. Airflow smoke patterns Of these tests, in order to insure that no disruptive air currents are penetrating the air inflow barrier (see Section 7.1) smoke flow tests must be performed at a minimum (only biological testing can confirm containment). These tests must result in the containment of smoke passed around the perimeter of the work access opening, as well as, no refluxing or drift of smoke within the interior of the cabinet. IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN WHO IS FAMILIAR WITH THE METHODS AND PROCEDURES FOR CERTIFYING BIOLOGICAL SAFETY CABINETS (SEE INSERT). AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE CABINET BE RECERTIFIED AT A MINIMUM ON AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE OR MAINTENANCE ACTION OR ANY TIME THE OPERATOR FEELS IT IS NECESSARY. Note that the LABGARD cabinets, filters and seals provide premium performance; Quality Control in both design and manufacturing assure superior reliability. However, protection to both product and operator is so vital that certification to the performance requirements should be accomplished as stated to ensure biological safety established by the factory standards. OM

36 Catalog Number Performance Specifications 1. Personal Protection 2. Product Protection 3. Air Cleanliness/Laminarity Labgard Class II, Type B2 Laminar Flow Biological Safety Cabinet Models NU /600 NU E/600E NU G/600G Catalog NU (E)(G) Nominal 4 foot (1.2m) NSF Std. No. 49 NSF Std. No. 49 Fed. Std. 209d, Class 10 OM Number NU (E)(G) Nominal 6 foot (1.8m) NSF Std. No. 49 NSF Std. No. 49 Fed. Std. 209d, Class 10 NSF Std. No. 49 Class Class 11, Type B2 Class 11, Type B2 Style of Cabinet Bench Top/Console w/base Stand/ Storage Cabinet Bench Top/Console w/base Stand/ Storage Cabinet Cabinet Construction All Welded Stainless Steel 16GA, Type 304 Pressure Tight Design All Welded Stainless Steel 16GA, Type 304 Pressure Tight Design Diffuser for Air Supply (Metal) Non-Flammable Non-Flammable HEPA Filter Seal Type: Supply Filter-99.99% Eff. on 0.3 Microns Exhaust Filter-99.99% Eff. on 0.3 Microns HEPEX Seal Neoprene, Spring loaded HEPEX Seal Neoprene, Spring loaded Fumigation per NIH/NSF Procedure Yes Yes Standard Services: Service Coupling (3/8 inch NPT) Gas Valve/Service Coupling(3/8 inch NPT) Duplex Outlet Optional Services: Gas Cocks 3/8" NPT Remote Controlled Valves** Ultraviolet Light Standard/Cup Sinks Cabinet Size Inches (mm): Height (Fully Assembled) Height (Minimum for Transport) Width Depth (with Control Center) Work Access Opening Inches (mm): Standard Opening Height Standard Inflow Velocity Work Zone Inches (mm): Height Width Depth Viewing Window Inches (mm): Standard is Tempered Sliding Glass Hinged Tempered Glass (optional) One, Right Sidewall One, Right Sidewall One, Left Front Faring Up to 3 ea. Sidewall Up to 3 ea. Sidewall One, Backwall Left or Right Work Surface 63 (1600) 60 (1524) 53 5/8 (1362) 32 7/8 (835) 8 (203) 105 FPM (.53 m/s) 25 1/2 (648) 46 3/8 (1178) 23 1/2 (597) Fully Closed to 18 1/2 (470) Open 8 (203) Access Opening One, Right Sidewall One, Right Sidewall One, Left Front Faring Up to 3 ea. Sidewall Up to 3 ea. Sidewall One, Backwall Left or Right Work Surface 63 (1600) 60 (1524) 77 5/8 (1972) 32 7/8 (835) 8 (203) 105 FPM (.53 m/s) 25 1/2 (648) 70 3/8 (1788) 23 1/2 (597) Fully Closed to 18 1/2 (470) Open 8 (203) Access Opening Required Exhaust CFM/CMH Standard: 754/ /1869 Plant Duct Static Pressure Eng/Metric 2.0 w.g./50.8mm w.g. 2.0 w.g./50.8mm w.g. Heat Rejected,BTU,Per Hour Negligible Negligible Electrical: 115V/(230V) Volts, AC (Hz) Amps: Blower/Lights Amps: Outlet Amps: Total 12 ft. Power Cord (one) Crated Shipping Weight: Net Weight U.L./U.L.-C Listed (115V only) 115, 60/(230, 50/60) 5/(3) 3/(3) 8/(6) 14 GA - 3 Wire, 15A 570 lbs./259 kg. 520 lbs./236 kg. U.L./U.L.-C Listed (115V only) 115, 60/(230, 50/60) 7/(4) 3/(3) 10/(7) 14 GA-3 Wire, 15A 760 lbs./345 kg. 710 lbs./322 kg. **Remote controlled valve handles project through front fairing. Decorative side panels are available to cover plumbing.

37 8.0 Operating the NU Electronic Airflow Control System Overview The electronic airflow control system is designed to service the control requirements of the NU-430 Biological Safety Cabinet. The control system is a self-contained microprocessor driven module that will perform the following functions: Control blower motor via solid state triac. Monitor, display and control downflow via dual thermister airflow probe. Monitor and display exhaust flow (inflow) via dual thermister airflow probe. Alarm setpoints high/low for error conditions (downflow and exhaust flow). Clock display (24 hours) and timer function. Control lights via solid state switch. Control outlets via solid state switch. Complete diagnostic functions. The NU-430 incorporating the use of the electronic airflow control system offers improved cabinet performance. The control system uses a dual thermister airflow probe in the downflow airstream to monitor and control airflow to setpoints. The control system automatically compensates for filter loading, voltage variances and other environmental effects. A second dual thermistor airflow probe in the exhaust airstream monitors for exhaust volume. Both downflow and exhaust are displayed on the front panel. The control system also monitors the sliding window position with a micro switch for both window height and window closed positions. The control system through the use of the front panel controls the on/off function of the fluorescent and ultraviolet lights (optional), outlets and blower. The control system also allows contact closure outputs for interaction with HVAC systems to optimize environmental performance. All the above functions are shown in a system block diagram (see Figure 1). Figure 1. System Block Diagram OM