BS-TB-004EB February TA Rotor. Used in the Beckman Coulter Allegra 25R and TJ-25 Centrifuges Beckman Coulter, Inc.

Transcription

1 BS-TB-004EB February 2014 TA Rotor Used in the Beckman Coulter Allegra 25R and TJ-25 Centrifuges Beckman Coulter, Inc.

2 SAFETY NOTICE This safety notice summarizes information basic to the safe use of the rotor described in this manual. The international symbol displayed above is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equipment is attempted. When you see the symbol on other pages of this publication, pay special attention to the safety information presented. Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor. Handle body fluids with care because they can transmit disease. No known test offers complete assurance that such fluids are free of micro-organisms. Some of the most virulent Hepatitis (B and C) viruses, HIV (I V), atypical mycobacteria, and certain systemic fungi further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this rotor without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials in nor handle or store them near the centrifuge. This rotor was developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a non-beckman Coulter centrifuge or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter s approval. Although rotor components and accessories made by other manufacturers may fit in the TA rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers components or accessories in the TA rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the components and accessories listed in this publication should be used in this rotor. Make sure that filled containers are loaded symmetrically into the rotor and that opposing bottles or tubes are filled to the same level with liquid of the same density. If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply all appropriate safety and decontamination procedures to the centrifuge and accessories as required. Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section on RUN SPEEDS. Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins in scratches and may open fissures in the rotor with continued use. 2

3 TA ROTOR 25 rav rmin rmax Axis of Rotation SPECIFICATIONS Maximum speed rpm Density rating at maximum speed g/ml Critical speed range* to 700 rpm Relative Centrifugal Field* at maximum speed At r max (96.0 mm) g At r av (64.5 mm) g At r min (33.0 mm) g Conditions requiring speed reductions see RUN SPEEDS Maximum imbalance of opposing loads grams Number of tube cavities Available bottles and tubes see Table 1 Nominal tube dimensions mm Nominal tube capacity (largest tube) ml Nominal rotor capacity ml Approximate acceleration time to maximum speed (fully loaded) sec Approximate deceleration time from maximum speed (fully loaded) sec Weight of fully loaded rotor kg (12.70 lb) Rotor and lid material aluminum *The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing through or running at the critical speed range is characterized by some vibration. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rω 2 ) to the standard acceleration of gravity (g) according to the following formula: RCF = rω g where r is the radius in millimeters, ω is the angular velocity in radians per second (2 π RPM /60), and g is the standard acceleration of gravity (9807 mm/s 2 ). After substitution: RCF = 1.12 r RPM

4 DESCRIPTION O-ring (393505) Lid (368304) Rotor Body This rotor has been manufactured in an ISO 9001 or facility for use with the specified Beckman Coulter centrifuges. The TA-14-50, rated for rpm, is a fixed angle rotor designed to centrifuge up to eight 50-mL bottles at a 25-degree angle to the axis of rotation. Used in Beckman Coulter Allegra 25R and TJ-25 centrifuges, the rotor develops centrifugal forces that can efficiently pellet cells from large volumes, or cell particles from tissue homogenates. Short column methods (such as partially filled tubes) may also be used to purify large quantities of virus in a cushion gradient. The rotor and lid are made of aluminum and are anodized for corrosion resistance the rotor is black and the lid is blue. A lubricated O-ring in the rotor maintains atmospheric pressure in the rotor during centrifugation. A tie-down screw is used to secure the rotor to the centrifuge drive hub. The centrifuge identifies rotor speed during the run by means of a magnetic speed sensor system in the rotor chamber and magnets imbedded in the rotor. The overspeed system ensures that the rotor does not exceed its permitted speed. Refer to the Warranty at the back of this manual for warranty information. PREPARATION AND USE Specific information about the TA rotor is given here. Information about the centrifuge is contained in the centrifuge manual, which should be used together with this manual for complete centrifuge, rotor, and accessory operation. NOTE Although rotor components and accessories made by other manufacturers may fit in the TA rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers components or accessories in the TA rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the components and accessories listed in this publication should be used in this rotor. 4

5 PRERUN SAFETY CHECKS Read the SAFETY NOTICE at the front of this manual before using the rotor. 1. Make sure that the rotor, lid, and all tubes or bottles and accessories are clean and show no signs of corrosion or cracking. 2. If fluid containment is required, use capped bottles or tubes. All containers carrying physiological fluids should be capped, and not overfilled, to prevent leakage. Verify that the bottles or tubes being used are listed in Table Check the chemical compatibilities of all materials used (refer to Chemical Resistances, publication IN-175). ROTOR PREPARATION For runs at other than room temperature, refrigerate or warm the rotor before the run for fast equilibration. 1. Be sure that the metal threads in the rotor are clean and lightly but evenly coated with Spinkote lubricant (306812). 2. Load the filled containers symmetrically into the rotor (see page 7 for tube and bottle information). If fewer than eight tubes are being run, they must be arranged symmetrically in the rotor (see Figure 1). Opposing tubes must be filled to the same level with liquid of the same density. 3. Put the lid in place and tighten it by hand. Figure 1. Typical Examples of Arranging Bottles or Tubes in the Rotor. Two, four, six, or eight containers can be centrifuged per run, if they are arranged in the rotor as shown. 5

6 OPERATION Precool the rotor in the centrifuge or in a refrigerator before use especially before short runs to ensure that the rotor reaches the set temperature. A suggested precooling cycle is a minimum of 30 minutes at 2000 rpm with the temperature set at 0 C. NOTE Temperatures may vary slightly between centrifuges. If sample temperature is crucial, test temperature settings on your instrument using water samples. For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. 1. Ensure that the rotor tie-down screw (368245) is in good condition and the threads are free of foreign matter. CAUTION Do not drop the rotor onto the drive shaft. The shaft can be bent or damaged if the rotor is forced sideways or dropped onto the shaft. 2. Center the rotor over the drive shaft and carefully lower it straight down. 3. Secure the rotor to the drive shaft with the tie-down screw. Tighten the screw by turning it to the right (clockwise) with the T-handle rotor wrench (368246). 4. Refer to the instrument instruction manual for centrifuge operation. See RUN SPEEDS, page 9, for information about speed limitations. 6

7 REMOVAL AND SAMPLE RECOVERY CAUTION If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories. 1. Using the T-handle rotor wrench, turn the tie-down screw to the left (counterclockwise) to release the rotor. 2. Remove the rotor by lifting it straight up and off the drive shaft. 3. Remove the rotor lid and remove tubes or bottles. BOTTLES AND TUBES The TA rotor uses the bottles and tubes listed in Table 1. Be sure to use only those items listed and observe the maximum fill volumes and speed limits shown. (Maximum fill volume is the maximum amount of fluid that can be centrifuged in the listed container.) To minimize the possibility of leakage from capped tubes or bottles, load the containers with sample, secure the caps, and precool the loaded labware to run temperature before beginning the run. Refer to Chemical Resistances for information on the chemical compatibilities of labware materials. Temperature Limits Plastic containers have been centrifuge tested for use at temperatures between 2 and 25 C. For centrifugation at other temperatures, pretest containers under anticipated run conditions. If plastic containers are frozen before use, make sure that they are thawed to at least 2 C before centrifugation. 7

8 Table 1. Available Bottles and Tubes for the TA Rotor. Use only the items listed here and observe fill volumes and maximum run speeds. Dimensions and Volume Tube Description Part Number Max. Fill Volume a (ml) Required Accessory Description Part Number Max Speed b / RCF mm 50 ml polypropylene bottle w/cap assembly (pkg/24) 40 none rpm g mm 50 ml polycarbonate bottle w/cap assembly (pkg/24) 40 none rpm g mm 50 ml polycarbonate bottle, screw-on cap (pkg/25) 38.5 none rpm g mm 50 ml polypropylene bottle, screw-on cap (pkg/25) 38.5 none rpm g mm 50 ml polycarbonate thickwall tube, snap-on cap (pkg/25) 36.5 white 29-mm cap red 29-mm cap green 29-mm cap yellow 29-mm cap blue 29-mm cap c c c c c rpm g mm 50 ml polypropylene thickwall tube, snap-on cap (pkg/25) 36.5 white 29-mm cap red 29-mm cap green 29-mm cap yellow 29-mm cap blue 29-mm cap c c c c c rpm g mm 50 ml mm 50 ml polycarbonate tube (pkg/25) polypropylene tube (pkg/25) 36.5 none rpm g 36.5 none rpm g a Above 20 C fill polypropylene tubes at least half full. Containers may be filled less than or equal to the maximum fill volumes provided here. b Maximum speeds listed are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter, but because of manufacturing variances no guarantee of performance or fit is expressed or implied. c Package of 25. Thickwall Tubes Thickwall polypropylene and polycarbonate tubes can be run partially filled (at least half filled) with or without caps, but all opposing tubes for a run must be filled to the same level with liquid of the same density. Do not overfill capless tubes; be sure to note the fill volumes and run speeds shown in Table 1. 8

9 Polycarbonate and Polypropylene Bottles Capped polycarbonate and polypropylene bottles can be run partially filled (not less than half full). Again, all opposing containers for a run must be filled to the same level with liquid of the same density. RUN SPEEDS SPEED The centrifugal force at a given radius in a rotor is a function of speed. Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields (RCF). When rotational speed is adjusted so that identical samples are subjected to the same RCF in two different rotors, the samples are subjected to the same force. The RCF at each speed is automatically calculated by the centrifuge software; if the RCF is entered, the centrifuge calculates the equivalent revolutions per minute (rpm). The maximum run speed listed in the rotor specifications is for operation when all conditions are within the standard specifications. Speeds must be reduced under the following circumstances: When certain glass or plastic tubes are used, reduce rotor speed to prevent tube breakage. Follow the manufacturers recommendations regarding maximum speed capacity for the tubes. Because the strength of tubes can vary between lots, and depend on handling and usage, Beckman Coulter highly recommends that you pretest these tubes in this rotor (using water instead of valuable sample) to determine optimal operating conditions. If nonprecipitating solutions more dense than 1.2 g/ml are centrifuged, the maximum allowable run speed must be reduced according to the following equation: reduced maximum speed = ( rpm) 1.2 g/ml ρ where ρ is the density of the tube contents. This speed reduction will protect the rotor from excessive stresses due to the added tube load. Note, however, that the use of this formula may still produce maximum speed figures that are higher than the limitations imposed by the use of certain tubes or adapters. In such cases, use the lower of the two figures. 9

10 Further speed limits must be imposed when self-forming-gradient salts are centrifuged, as the equation does not predict concentration limits/speeds that are required to avoid precipitation of salt crystals. CARE AND MAINTENANCE NOTE If glass tubes break, remove the glass very carefully from the adapter or cavity. Imbedded glass particles that remain in cavities or adapters can cause tube failure during subsequent runs. MAINTENANCE Check for Corrosion O-ring (393505) Lid (368304) Rotor Body Do not use sharp tools on the rotor, as they can scratch the anodized surface. Corrosion begins in scratches and may open fissures in the rotor with continued use. Periodically (at least monthly) inspect the rotor, especially inside cavities, for rough spots, cracks, pitting, white powder deposits (frequently aluminum oxide), or heavy discoloration. If any of these signs are evident, do not run the rotor. Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center. Before using the tie-down screw, check it for damage such as stripped threads. Replace it if it is damaged. Regularly apply a thin, even coat of Spinkote lubricant (306812) to the rotor drive hole to prevent rotor sticking. Refer to Chemical Resistances for the chemical compatabilities of rotor and accessory materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center. 10

11 CLEANING Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred. Do not allow corrosive materials to dry on the rotor. Rotor Cleaning Kit (339558) Under normal use, wash the rotor frequently (at least weekly) to prevent buildup of residues. If the rotor is left in the centrifuge for long periods of time, remove it at least once a month for cleaning and lubrication. 1. Remove the O-ring and wash the rotor, lid, and O-ring in a mild detergent, such as Beckman Solution 555 (339555), that won t damage the rotor. The Rotor Cleaning Kit (339558) contains two plastic- coated brushes and two quarts of Solution 555 for use with rotors and accessories. Dilute the detergent 10 to 1 with water. NOTE Do not wash the rotor components or accessories in a dishwasher. Do not soak components in detergent solution for long periods of time, such as overnight. Lid (368304) 2. Rinse the cleaned rotor and components with distilled water. O-ring (393505) 3. Air-dry the rotor and lid upside down. Do not use acetone to dry the rotor. 4. Lightly but evenly lubricate the rotor drive-hole threads with Spinkote (306812). Rotor Body 5. Apply a thin, even coat of silicone vacuum grease (335148) to the O-ring, then replace it in the groove in the outer rim of the rotor. Clean metal threads every 6 months, or as necessary. Use a brush and concentrated Solution 555. Rinse and dry thoroughly, then lubricate lightly but evenly with Spinkote to coat all threads. 11

12 DECONTAMINATION If the rotor (and/or accessories) becomes contaminated with radioactive material, decontaminate it using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum: RadCon Surface Spray or IsoClean Solution (for soaking),1 and Radiacwash.2 NOTE IsoClean can cause fading of colored anodized surfaces. Use it only when necessary and remove it promptly from rotor surfaces. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. Consult your laboratory safety officer regarding the proper decontamination methods to use. If the rotor or accessories are contaminated with toxic or pathogenic solutions, follow appropriate decontamination procedures. Check Chemical Resistances to be sure the decontamination method will not damage any part of the rotor. STERILIZATION AND DISINFECTION The rotor and all rotor components can be autoclaved at 121 C for up to one hour. Remove the lid from the rotor and place the rotor, lid, and O-ring in the autoclave upside down. Ethanol (70%)3 or hydrogen peroxide (6%) may be used on all rotor components, including those made of plastic. Bleach (sodium hypochlorite) may be used, but may cause discoloration of anodized surfaces. Use the minimum immersion time for each solution, per laboratory standards. 1 In U.S.A., contact Nuclear Associates (New York); in Eastern Europe and Commonwealth States, contact Victoreen GmbH (Munich); in South Pacific, contact Gammasonics Pty, Ltd. (Australia); in Japan, contact Toyo Medic Co. Ltd. (Tokyo). 2 In U.S.A., contact Biodex Medical Systems (Shirley, NY); internationally, contact the U.S. office to find the dealer nearest you. 3 Flammability hazard. Do not use in or near operating centrifuges. 12

13 While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. Refer to publication IN-192, included in each box of tubes or bottles, for tube and bottle sterilization and disinfection procedures. TUBE BREAKAGE If a glass bottle or tube breaks, remove the glass very carefully from the rotor. Imbedded glass particles that remain in the rotor cavities or adapters can cause tube failure during subsequent runs. Clean the rotor thoroughly immediately following a bottle or tube breakage. STORAGE When the rotor is not in use, store it in a dry environment (not in the centrifuge) with the rotor lid removed to allow air circulation so moisture will not collect in the tube cavities. 13

14 RETURNING A ROTOR RGA Before returning a rotor or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. This RGA form, which may be obtained from your local Beckman Coulter sales office, should contain the following information: rotor serial number, history of use (approximate frequency of use), reason for the return, original purchase order number, billing number, and shipping number, if possible, name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory, and name and phone number of the person to be notified about repair costs, etc. To protect our personnel, it is the customer s responsibility to ensure that the parts are free from pathogens and/or radioactivity. Sterilization and decontamination must be done before returning the parts. Smaller items (such as tubes, bottles, etc.) should be enclosed in a sealed plastic bag. All parts must be accompanied by a note, plainly visible on the outside of the box or bag, stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach this notification will result in return or disposal of the items without review of the reported problem. Use the address label printed on the RGA form when mailing the rotor and/or accessories. Customers located outside the United States should contact their local Beckman Coulter office. 14

15 SUPPLY LIST NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at in the United States, or by contacting your local Beckman Coulter office. Call Beckman Coulter Sales ( in the United States; worldwide offices are listed on the back cover of this manual) for detailed information on ordering parts and supplies. For your convenience, a partial list is given below. REPLACEMENT ROTOR PARTS TA rotor assembly Lid assembly O-ring, rotor (large) O-ring, lid (small) Tie-down screw T-handle rotor wrench SUPPLIES Bottles, tubes, and accessories see Table 1 Spinkote lubricant (2 oz) Silicone vacuum grease (1 oz) Rotor Cleaning Kit Beckman Solution 555 (1 qt)

16 BENCHTOP ROTOR WARRANTY Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election, by replacement, any defects of material or workmanship which develop within seven (7) years after delivery of a benchtop centrifuge rotor to the original buyer by Beckman Coulter or by an authorized representative, provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use. Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this warranty, Beckman Coulter will supply free of charge all centrifuge parts required for repair. 2. This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual. 3. This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor characteristics or operated in a Beckman Coulter centrifuge that has been improperly disassembled, repaired, or modified. 4. Thermoplastic rotors or components used in some benchtop centrifuges are warranted for one (1) year from date of purchase. CONDITIONS 1. Except as otherwise specifically provided herein, this warranty covers the rotor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to (i) tubes, (ii) tube caps, (iii) tube adapters, or (iv) tube contents. DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR- RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT- ABILITY AND THAT NEITHER BECKMAN COULTER, INC. NOR ITS SUPPLIERS SHALL HAVE ANY LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR REPLACE- MENT OF THE PRODUCT. TRADEMARKS Beckman Coulter, the stylized logo, and Optima are trademarks of Beckman Coulter, Inc. and are registered with the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

17 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, California U.S.A. Sales and Service: Internet: Printed in USA 2014 Beckman Coulter, Inc. All rights reserved