Operation Manual Cole-Parmer MPR16 Multipurpose Refrigerated and MP16 Multipurpose Ambient Centrifuges Models , -25, -50, -55

Transcription

1 Operation Manual Cole-Parmer MPR16 Multipurpose Refrigerated and MP16 Multipurpose Ambient Centrifuges Models , -25, -50, -55 CE 1065z76_MAN_Rev Cole-Parmer Instrument Company, LLC

2 Table of Contents Preface 1 Introduction 1 Unpacking 1 Precautions 1 Setup Conditions 2 Preparation 2 Hazardous Substances 2 Operation & Symbols 3 Features & Advantages 4 Technical Specifications Centrifuge 5 Technical Specifications Rotors, Adapters, and Reducers 6 Transport and Set Up 7 Making Sure the Rotor is Balanced 9 Installation/Fixation for a Fixed-Angle Rotor 10 Installation/Fixation for a Swing-Out Rotor 11 Programming & Operation 12 Maintenance & Care 14 Troubleshooting 18 Contact Information 20

3 Preface Before starting to use the Cole-Parmer series of multipurpose centrifuges, read through this instruction manual carefully and follow the instructions. The information contained in this instruction manual is the property of Cole-Parmer Instrument Company, LLC; it is forbidden to copy or pass on this information without explicit approval. Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the warranty. This centrifuge is a laboratory product used to separate substance mixtures of different densities. This centrifuge should be operated by trained personnel only. Introduction Cole-Parmer series of benchtop multipurpose centrifuge (MPR16 refrigerated models and -25; MP16 ambient models and -55) offers rugged construction without sacrificing reliable performance. Extra-thick stainless steel chamber and zinc-coated alloy and steel frame increase durability for inorganic separations requiring strong solvents. Industrial-grade inverter drive (VFD) and brushless motor provide a long service life. User-accessible service sections allow onsite maintenance. Refrigerated models feature a highly accurate PID controller which allows better overall temperature control, achieving the desired set temperature. In addition, the large, efficient CFC-free compressor uses less power and lasts longer. LED displays show temperature, rpm, and time. Programmable memory supports 10 programs including acceleration and deceleration rates, allowing you to quickly perform routine tasks. The included fixed-angle rotor (model ) accommodates eight 50-mL tubes ideal for civil, environmental, and water supply engineering applications. Increase versatility by using optional rotors; call for details. Unpacking Check individual parts against the list of items below. If anything is missing or damaged, please contact Cole-Parmer immediately. 1. Multipurpose centrifuge 2. Fixed-angle rotor (model ) 3. Rotor installation kit 4. Power cord 5. User manual Precautions Observe the safety instructions. Not following these instructions can cause damage. The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with fatal consequences. The centrifuge should be operated by trained personnel only. In order to ensure safe operation of the Cole-Parmer centrifuge, general safety regulations must be followed: refer to the regulations in your country. 1

4 Setup Conditions Plug the centrifuge only into sockets which have been properly grounded. Turn off the centrifuge at the main switch. The mains plug must be freely accessible at all times. Press the STOP key to shut the centrifuge down. Pull out the power supply plug or disconnect the power supply in an emergency. Maintain a radius of at least 12 in. (30 cm) around the centrifuge. Do not place any dangerous substances within the security zone. Set up in a well-ventilated environment, place on a level and rigid surface with adequate load-bearing capacity. Preparation It is the general obligation of the operator to make sure that the proper protective clothing is used. Refer to the "Laboratory Biosafety Manual" of the World Health Organization (WHO) and the regulations in your country. Do not touch the mechanical components of the rotor and do not make any changes to the mechanical components. Do not touch the electronic components of the centrifuge or alter any electronic or mechanical components. Use only with rotors which have been properly installed. Follow the instructions on pages Do not use rotors which show any signs of corrosion and/or cracks. Contact your supplier if the centrifuge and rotors are in any conspicuous condition, e.g. showing signs of corrosion and/or cracks. Use only with rotors which have been loaded properly. Never overload the rotor. Always balance the samples. Use only rotors and accessories for this centrifuge which have been approved by the supplier. Exceptions to this rule are commercially available glass or plastic centrifuge tubes, provided they have been approved for the speed or the RCF value of the rotor. Make sure the rotor is locked properly into place before operating the centrifuge. Implement of special measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it is running. If used for foodstuffs machinery, for cosmetics or pharmaceutical products, only use closed or aerosol-tight containers for centrifugation. Hazardous Substances Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and vessel have to be cleaned carefully. Do not centrifuge explosive or flammable materials or substances which could react violently with one another. The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone environment. Do not centrifuge inflammable substances. 2

5 Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions. If centrifuging any hazardous materials, mind the Laboratory Biosafety Manual of the WHO. When centrifuging microbiological samples from the Risk Group II, aerosol-tight biological seals must be used. For materials in a higher risk group, extra safety measures have to be taken. If toxins or pathogenic substances have gotten into the centrifuge or its parts, appropriate disinfection measures have to be taken. See "Disinfection" on page 15. Highly corrosive substances which can cause material damage and impair the mechanical stability of the rotor should only be centrifuged in corresponding protective tubes. If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately. Operation & Symbols Never use the centrifuge if parts of its cover panels are damaged or missing. Never start the centrifuge when the door is open. Do not move the centrifuge while it is running. Do not lean on the centrifuge. Do not place anything on top of the centrifuge during a run. This applies also for the rotor landing pad. Never open the door until the rotor has come to a complete stop and this has been confirmed in the display. Emergency door release may be used in emergencies only to recover samples from the centrifuge, e.g. during a power failure (see section "Mechanical Emergency Door Release on page 18). Do not open the centrifuge, while it is running. In any case of mechanical breakdown rotor failure, like burst of the rotor, breaking bottles or shaft crack, the centrifuge is not aerosol-tight. In case of rotor failure, the centrifuge can be damaged. Leave the room immediately. Symbols used on the centrifuge: This symbol refers to general hazards. CAUTION! means that material damage could occur. WARNING! means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. & This symbol refers to information on hazards, described within this manual. 3

6 Features & Advantages Built to last, strong construction yet offering a sleek modern design that will fit into any modern laboratory. No compromise on quality only the best components are used in the manufacturing process. User Features Large, bright blue LED display Advantages Clarity and ease of use 10 acceleration rates, 10 deceleration rates Sample assistance 10 memory programs Multiple users Timer 0 to 99 minutes and Hold in 10-sec increments Pulse short run Run in speed (rpm) or RCF (G) in 10-rpm increments Sound <60 db Orientation acceleration rate Refrigerated models PID controlled Mechanical Features Extra-thick stainless steel bowl Port to lid Alloy and steel frame with composites World-leading industrial-grade Inverter Quality European brushless motor High technology airflow (ambient models) Safety Features Multipoint lid locking Emergency lid-release Lid gas strut Lid lock detection Imbalance detection Overspeed sensor Set inverter values Barrier ring Motor overheat sensor Total flexibility Fast pelleting Accuracy Quiet Prevents initial sample side deposits Accuracy Advantages Easy to clean and rust free Tachometer speed certification Strong light and quiet Reliability and strength Quiet, cool, and reliable Cooler running Advantages Lid safety Power failure release Lid dropping safety Lid must be shut in order to operate Easy eye volume loading Safe detection of speed Electronic safety of speed Extra metal protection of chamber Safe motor protection Note: All centrifuges have user-accessible service sections to access all safety parameters 4

7 Technical Specifications Centrifuge Model (display indicative only) Speed: 500 to 6000 rpm, in 10 rpm steps RCF max: 4800 RCF x g Max capacity: 1 L Temperature (refrigerated models): 9 to 40 C (15.8 to 104 F), PID controlled to ±1 C Acceleration rates: 10 programs Deceleration rates: 10 programs Memory: 10 programs Timer: 0 to 99 minutes and Hold, in 30 second steps Dimensions (W x H x D): Refrigerated models: 17.7 x 12.4 x 25 in. (45 x 31.5 x 63.5 cm) Ambient models: 15.7 x 12.2 x 19.7 in. (40 x 31 x 50 cm) Weight without rotor: Refrigerated models: 138 lb (62.2 kg) Ambient models: 70 lb (31.6 kg) Power: Refrigerated: 110 VAC, 60 Hz (model ); 230 VAC, 50 Hz (model ) Ambient: 110 VAC, 60 Hz (model ); 230 VAC, 50 Hz (model ) Rotor included: Fixed-angle model Additional rotors are available upon request. 5

8 Technical Specifications Rotors, Adapters, and Reducers Rotors Catalog number (included) (not included) (not included) Rotor model BRK5308 BRK5510 BRK5100 Description Fixed angle, polypropylene, Swing out, aluminum Fixed angle, polypropylene 8 x 50 ml 4 x 250 ml 6 x 100 ml Buckets (set of 4) No Included No Sealed lid No Available No Tube size max 30 x 120 mm 45 x 110 mm 85 x 130 mm Minimum speed 500 rpm 500 rpm 500 rpm Maximum speed 6000 rpm 4000 rpm 6000 rpm Maximum RCF x g Radius max (cm) Sample tube angle Acceleration time (sec) Deceleration time (sec) Autoclaveable (frequency) 121 C (20) 121 C (20) 121 C (20) Minimum temperature 4 C (20 C max) 4 C (20 C max) 4 C (20 C max) for refrigerated models Tube Adapters Catalog number (set of 4) Model Tube type AN0650 Nalgene / Oakridge, round bottom, 50 ml, 29 x 107 mm Reducers Catalog number (set of 4) Model Description RM15 [15ml] Max tube size 17 x 120 mm Catalog number (set of 4) Model Description RM25 [25ml] Max tube size 25 x 100 mm 6

9 Transport and Set Up Location The centrifuge should only be operated indoors. The set-up location must fulfill the following requirements: A safety zone of at least 12 in. (30 cm) must be maintained around the centrifuge. People and hazardous substances must be kept out of the safety zone while centrifuging. The supporting structure must be stable and free of resonance. The supporting structure must be suitable for horizontal setup of the centrifuge. The centrifuge should not be exposed to heat and strong sunlight. The set-up location must be well-ventilated at all times. WARNING! UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct sunlight. Transporting the Centrifuge Due to its weight, the centrifuge should be carried by several people. Always remove the rotor before moving the centrifuge. Always lift the centrifuge on both sides. Never lift the centrifuge by its front or back panel. Transport the centrifuge upright and if at all possible in its packaging. NOTICE Store the original centrifuge packaging. Contact a shipping company for the transport or inform the customer service. Always remove the rotor before moving the centrifuge. If you do not remove the rotor, you might damage the centrifuge drive or centrifuge spindle. Aligning the Centrifuge The horizontal alignment of the centrifuge must be checked every time after moving it to a different location. The supporting structure must be suitable for horizontal setup of the centrifuge. CAUTION! If the centrifuge isn't leveled, imbalances can occur and the centrifuge can be damaged. Do not place anything under the centrifuge feet to level the centrifuge. 7

10 Mains Connection 1. Turn off the power supply switch located on the left side of the centrifuge (press "0"). Power supply switch 2. Plug the centrifuge into grounded electrical sockets only. 3. Check whether the cable complies with the safety standards of your country. 4. Make sure that the voltage and frequency correspond to the figures on the rating plate. 5. Establish the connection to the power supply with the connecting cable. Storage WARNING! When removing the centrifuge and accessories from use you have to clean and if necessary disinfect or decontaminate the entire system. If in doubt, contact the supplier. Before storing the centrifuge and accessories, it must be cleaned and if necessary disinfected and decontaminated. Store the centrifuge in a clean, dust-free location. Be sure to place the centrifuge on its feet. Avoid direct sunlight. Shipping the Centrifuge Before shipping the centrifuge, please bear the following in mind: The centrifuge must be clean and decontaminated. You must provide proper documentation confirming the decontamination. If in doubt, contact the Cole-Parmer. 8

11 Making Sure the Rotor is Balanced In centrifugation, it is vitally important that rotors are balanced correctly. Any slight imbalance when weighing or placing your sample in the rotor could be exaggerated once the centrifuge is spinning. Please note the examples shown below of correct loading of samples: Incorrect Incorrect Correct 9

12 Installation/Fixation for a Fixed-Angle Rotor Installation kit includes: M6 washer Rotor fixing screw, M6 x 20 mm Allen key Step 1. Place the rotor on the shaft of the motor. Step 2. Place the washer over the central hole on the rotor. Step 3. Place the rotor fixing screw into the central hole of the rotor. Step 4. Hand tighten the rotor fixing screw with the Allen key. CAUTION! Please ensure that the fixing installation is checked and hand-tightened on a regular basis. Failure to follow these instructions could cause serious damage to the centrifuge and possible injury to persons nearby and would make the warranty invalid. 10

13 Installation/Fixation for a Swing-Out Rotor Installation kit includes: M6 washer Rotor fixing screw, M6 x 20 mm Allen key Step 1. Place the rotor on the shaft of the motor. Step 2. Place the M6 washer over the central hole on the rotor. Step 3. Place the rotor fixing screw into the central hole of the rotor. Step 4. Hand tighten with Allen key. Step 5. Ensure buckets are fully inserted before use CAUTION! Please ensure that the fixing installation is checked and hand-tightened on a regular basis. Failure to follow these instructions could cause serious damage to the centrifuge and possible injury to persons nearby and would make the warranty invalid. 11

14 Programming & Operation Please make sure the lid is kept closed at all times, otherwise temperature control cannot be held. Allow at least 20 minutes for below ambient temperatures to be reached and stabilized. Wipe the bowl regularly to remove moisture that can collect due to the dew point effect. Note: For above ambient temperatures, please lower the temperature to 4 C first (to work the internal gases). Once this temperature has been reached, then raise 20 C to 40 C as required. How to Program the Centrifuge To set temperature: Press up or down arrows below temp display. To set speed in rpm: Press up or down arrows below speed display. To set time: Press up or down arrows below time display. To set RCF x g: Press and hold the Function button, and press Lid Open button. Note the direction of blue dot in the speed display: it moves to top left for RCF program, bottom right for rpm. To set acceleration or deceleration rates: Press and hold the Function button, then press Speed Down arrow for acceleration or press Speed Up arrow for deceleration (brake). Both show 0 to 9 inclusive, with 9 being the highest rate. To set memory and store (first set speed or RCF and time): Press and hold Function button, then press Pulse button to select numbers (0 to 9 inclusive). Press Time Down arrow to store. To recall memory (first select memory number, see above): Press and hold Function button, then press Time Up arrow to recall memory. Display showing programmed run of 4 C, 1500 rpm, 10 minutes. Display showing actual bowl temperature (19 C) and programmed run. Two dots indicate preconditioning run in progress which will run at half the programmed speed. 12

15 Before Operation If the centrifuge has been in operation for some time, over an hour, at the same temperature, then equilibration is not necessary. However, if the centrifuge has just been turned on then we recommend the following for the best and most accurate results. Below ambient or 18 C (4 to 18 C / 39 to 64 F) Refrigerated Models Only Turn on the unit. Select your temperature, make sure the rotor is in position and firmly fixed. Close the lid. Wait at least 20 minutes. Whilst waiting place your samples, with tubes in refrigerated unit at the desired temperature. After your set temperature has been reached, use the precondition mode by pressing down the Function button and Start/Stop button together. Two dots will appear on the display showing that precondition mode has been activated. Precondition mode runs the centrifuge at half programmed speed to ensure full mixing of the chamber air and therefore equilibrates all areas of the rotor and parts for maximum efficiency. Once this has finished, lift the lid and load samples in position. See page 9 for correct loading procedures. How to Operate the Centrifuge Load samples, fit lid (if applicable). Set Speed or RCF x g, then designated time. Close the centrifuge lid. Press down firmly on both left and right sides. Press Start button (bottom left), the motor will now accelerate to the designated setting. When time has counted down (time display right), the rotor will decelerate (brake). According to your programmed setting (9 being the fastest), the rotor will stop and OpEn will appear on the display. Press Lid open button and retrieve your samples. You can stop the run at any time by pressing the Start/Stop button. How to Use the Pulse (short run) Feature This method allows very short runs mainly for pelleting purposes. Set your desired speed or RCF x g. Time is not relevant. Load your samples. Fit lid (If applicable). Press Pulse button (bottom right) keep held down. You will see the Time display counting up in seconds. As long as you keep the button held down, the time will increase. On releasing the Pulse button, the centrifuge will brake. Press Lid open button when OPEN appears on the display and retrieve your samples. CAUTION! Follow all safety instructions on previous pages. Always load samples evenly. 13

16 Maintenance & Care Cleaning Intervals For the sake of personal, environmental, and material protection, it is your duty to clean and if necessary disinfect the centrifuge on a regular basis. See recommended cleaning intervals: Rotor chamber... Daily or when polluted Rotor... Daily or when polluted Accessories... Daily or when polluted Cabinet... Once per month Ventilation holes... Every six months CAUTION! Use only approved cleansers. Refrain from using any other cleaning or decontamination procedure than those recommended here. If you are not entirely sure that the intended procedure or cleanser is safe for the equipment, contact the supplier. Cleaning Clean centrifuge and accessories as follows: Turn off the centrifuge and remove power supply plug. Open the centrifuge lid. Grab the rotor handle with both hands and lift it vertically from the centrifuge spindle. Remove the centrifuge tubes and adapters. Use a neutral cleaning agent with a ph value between 6 and 8 for cleaning. Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions, or scrubbing powder. Rinse the cavities out thoroughly. Use a soft brush without metal bristles to remove stubborn residue. Afterwards rinse with distilled water. Dry all of the rotors and accessories after cleaning with a soft cloth or in a warm air cabinet at a maximum temperature of 50 C. Higher temperatures could damage the material and shorten the lifetime of the parts. After cleaning, treat the entire surface of aluminum parts with corrosion protection oil. Also treat the cavities with oil. Treat the bolt of the swing out rotor with bolt grease. Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down CAUTION! When cleaning, do not allow liquids, especially organic solvents, to get on the drive shaft, the bearings, or the locks. Organic solvents break down the grease in the motor bearing; the drive shaft could lock up. 14

17 Disinfection Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation. Decontaminate the affected parts immediately. Take other precautions if need be. The rotor chamber and the rotor should be treated preferably with a neutral disinfectant. If unsure, contact the supplier for questions regarding the use of other disinfectants. Observe the safety precautions and handling instructions for the cleaning agents used. Disinfect the rotor and accessories as follows: Turn off the centrifuge and remove power supply plug. Open the centrifuge lid. Grab the rotor handle with both hands and press against the Auto-Lock button. At the same time, pull the rotor directly upwards it from the centrifuge spindle. Remove the centrifuge tubes and adapters and dispose of them or disinfect them. Treat the rotor and accessories according to the instructions for the disinfectant. Adhere strictly to the given application times. Be sure the disinfectant can drain off the rotor. Rinse the rotor and rotor li d thoroughly with water and then rub down. Dispose of the disinfectant according to the applicable guidelines. Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C. After cleaning, treat the entire surface of aluminum parts with corrosion protection oil. Also treat the cavities with oil. Treat the bolt of the swing out rotor with bolt grease. WARNING! Infectious material can get into the centrifuge when a tube breaks or as a result of spills. Keep in mind the risk of infection when touching the rotor and take all necessary precautions. In case of contamination, make sure that others are not put at risk. 15

18 Decontamination Decontaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation. In case of contamination, make sure that others are not put at risk. Decontaminate all affected parts immediately. Take other precautions if need be. Before using any cleaning or decontamination methods except those recommended by the supplier, users should check with the supplier that the proposed method will not damage the equipment. For general radioactive decontamination, use a solution of equal parts of 70% ethanol, 10% SDS and water. Disinfect the rotor and accessories as follows: Turn off the centrifuge and remove power supply plug. Open the centrifuge lid. Grab the rotor handle with both hands and press against the Auto-Lock button. At the same time, pull the rotor directly upwards it from the centrifuge spindle. Remove the centrifuge tubes and adapters and dispose of them or disinfect them. Rinse the rotor first with ethanol and then with deionized water. Adhere strictly to the given application times. Be sure the decontamination solution can drain off the rotor. Rinse the rotor and accessories thoroughly with water. Dispose of the decontamination solution according to the applicable guidelines. Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C. After cleaning, treat the entire surface of aluminum parts with corrosion protection oil. Also treat the cavities with oil. Treat the bolt of the swing out rotor with bolt grease. WARNING! Radioactive material can get into the centrifuge when a tube breaks or as a result of spills. Keep in mind the risk of infection when touching the rotor and take all necessary precautions. Autoclaving Clean the rotor before autoclaving and rinse thoroughly with distilled water. Remove all accessories (tubes, adapters) from the rotor. Place the rotor on a flat surface. Rotors and adapter can be autoclaved at 121 C (250 C) with a maximum permissible autoclave cycle is 20 minutes. CAUTION! No chemical additives are permitted in the steam. Never exceed the permitted temperature and duration when autoclaving. 16

19 Servicing We recommend having the centrifuge and accessories serviced once a year by an authorized service technician; contact the supplier for details. The service technician checks the following: Electrical equipment Suitability of the set-up site Door lock and safety system Rotor Installation/fixation of the rotor and the centrifuge spindle Shipping and Disposing For proper disposal of the centrifuge, refer to the regulations in your country. If in doubt, contact the supplier. Also refer to "Transporting the Centrifuge" and "Shipping the Centrifuge" on pages 7-8. For the countries of the European Union the disposal is regulated by the European Union's Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. WARNING! When removing the centrifuge and accessories from use for disposal, you must clean and if necessary disinfect or decontaminate the entire system. If in doubt, contact Cole-Parmer. Performance Recommendations Always keep buckets and adapters clean and free from moisture at all times, this will help prevent corrosion and keep them in good, safe operating condition. Use only supplier-approved accessories with your product. Always ensure samples are distributed in a diametric pattern to ensure the unit runs balanced. Rotor pins should be kept clean and lubricated on a regular basis. It is recommended that your centrifuge is switched off and the lid left open overnight to ensure that any moisture build up can evaporate. Always ensure that lids and accessories are correctly tightened before running the unit. Ensure that all buckets are present in the rotor when running your centrifuge, as using the unit with an unbalanced rotor can cause distortion and excessive wear. Do not use any kind of scourers or abrasive material to clean the buckets and ensure that a ph neutral cleaner is always used. It is recommended that regular maintenance and cleaning of the unit and accessories is carried out. Be sure to make note of the rotor expiration date which can be found on the underside of your rotor. 17

20 Troubleshooting Mechanical Emergency Door Release During a power failure, you will not be able to open the centrifuge lid with the regular electric lid release. A mechanical override is provided to allow sample recovery in the case of an emergency. However, this should be used only in emergencies and after the rotor has come to a complete stop WARNING! The rotor can still be spinning at high speed. If touched, it can cause serious injuries. Always wait until the rotor has come to a complete stop without braking. The brake does not work when there is no current. The braking process lasts much longer than usual. Proceed as follows: 1. Make sure the rotor has stopped (view port in the lid). WARNING! Never use your hands or other tools to brake the rotor. 2. Pull out the power supply plug. 3. On the bottom of the housing is one black plastic plug which you can pry out of the side plate with a screwdriver. It is located below the front side of the centrifuge. It is reachable with a screwdriver. Pull the release cord attached to it to trigger the mechanical door release. The door will open and the samples can be removed. Open the front door. Below front side Mechanical Emergency Door Release on the downside 4. Place the centrifuge so that you have access to the downside of the front panel. CAUTION! Due to its weight, the centrifuge should be carried by several people. Always lift the centrifuge from both sides. 5. Push the cord back into the centrifuge and mount the plug. 6. Reconnect the centrifuge once the power has been restored. Switch on the centrifuge. 7. Press the OPEN key to have the door locks operative again. 18

21 Codes Shown on the LED Display OPE n You may now open the lid. WAI t Wait 5 seconds, press Lid Open again. L Id? Close the lid. T Ach o? Check that your tubes are not too long and fouling the lid or if anything else is stopping the rotation of the rotor. bal? An imbalance has occurred. Please check the tubes are diagonally opposite and of the same weight. Check if the tubes are broken; if so use better quality, or if glass reduce the speed to the manufacturer s recommended G force. NOTE: If druch or drlue (drive) show, please contact Cole-Parmer. Test Routine (all LED models) This allows you to turn the buzzer on or off and to check the sensors for Tachometer, Rotor recognition, Lid open, and Imbalance detector. Display shows 0 or 1 as you change settings or perform diagnostic checks. Step 1. Centrifuge lid must be in open position. Power must be off at rear switch. Step 2. Press Function button (and keep held down) while turning on at rear switch. When display reads test, release Function button. Step 3. Press Time Up arrow to scroll through test functions. As you scroll, digits in the time segment of the display will change. Use Speed Up arrow to change settings or enter sensor diagnostic menu. Buzzer off/on (0/1).... Time = 34 Speed in 10/100 (0/1) rpm increments Time = 35 EEPROM check.... Time = 36 LED display check. Speed Up arrow enters sensor diagnostics... Display = Sensor diagnostics Time = 38 A. Rotor recognition/tachometer sensors turn rotor until 0 turns to 1 for first two digits of speed display. B. Imbalance detector push rotor to one side until 0 turns to 1 in the last digit of speed display. C. Lid open detector close lid. Third digit of speed display will turn from 0 to 1. Buzzer test... Display = Bu2t St Watchdog test.... Display = Do9t St Hello should display last, then unit automatically exits Test Routine Display = HELLO 19

22 Error Messages and Troubleshooting tacho shown on the display Note this does not necessarily mean a Tachometer or board problem. First check that the sample tubes are not too tall and preventing the rotor to move when the lid is closed Check the rotor and motor for movement; they must rotate smoothly Check the tachometer on the underside for dust or intrusions to the sensor If none of the above, contact Cole-Parmer. bal shown on the display An imbalance has occurred. Check the sample tubes for equal and opposite positioning in rotor. See Programming & Operation section for correct loading and instructions. Turn off the centrifuge at the power inlet and wait 2 minutes. If bal still shows on display the detector is damaged. If none of the above, contact Cole-Parmer. Speed will not go beyond 2000 rpm Electronics have set the rotor into safety mode Check the sensor on top of the motor, check the magnets are clean (wipe with a dry cloth) on underside of rotor. If none of the above, contact Cole-Parmer. Noisy Balance the samples in the rotor, check the lid seals are in place and in good order. Lid support is weak Gas strut failure, contact Cole-Parmer. For Information, Contact: Toll-Free: Phone: Fax: ColeParmer.com Note: If you need to contact Cole-Parmer for troubleshooting or repair, please provide the model number and serial number of your centrifuge. 20