Sample preparation Methods Analysis. Biodiesel Analysis guide.

Transcription

1 Sample preparation Methods Analysis Biodiesel Analysis guide

2 Biodiesel is taking its place as one of the new cleaner and greener solutions required to meet the world s growing energy needs. New feedstocks, production techniques and applications are continuing to drive the production capabilities of biodiesel to meet consumer demand. This is increasing the analysis required by biodiesel producers. This guide has been created as a resource for biodiesel analysis. Inside you will find method information, products and solutions designed to ensure you can effectively and reliably complete fuels testing according to either ASTM International or European National (EN) methods. The latest information about our products and applications can always be found at our website: Sample preparation Methods Analysis 2

3 Introduction to biodiesel analysis The rising price of oil is driving fuel prices to new highs, causing substantial global economic impacts. This economic situation combined with the desire for cleaner and greener energy solutions has created the demand for new fuels. Companies and consumers are looking for alternative ways of deriving fuel from available sources. One alternative fuel achieving both environmental and economic requirements is biodiesel. Biodiesel is diesel derived from vegetable oils, animal fats and waste food by-products, usually cooking oil. Major investment is currently being made into the development of algae and other specifically bred plant crops that will increase yield and enable biodiesel to become a major component of our energy infrastructure. Product process The most widely accepted form of biodiesel production is trans-esterification, where typically oil is mixed with a solvent, usually methanol, and catalyzed by sodium methoxide, which causes the oil to be split into biodiesel and glycerine by-products. The biodiesel will then be processed for use in vehicles and the waste products split-off and sold separately. Glycerols, the major waste components, need to be minimized to trace levels within the fuel to prevent engine malfunction. Both the ASTM International and European National (EN) standards for biodiesel fuel certification require the fuel to be tested for total glyercol content before it can be released for sale. The European biodiesel standard mandates the completion of additional tests such as methanol and FAMEs. Biodiesel guide contents Trajan consumables - GC selection 6-7 Sample preparation 8 Capillary columns - BPX-BIOD GC 9 Method library: ASTM 6584 / EN1415 free glycerine, mono-glycerides, di glycerides, tri-glyerides - EN1413 Determination of ester and linolenic acid methyl ester contents - EN1411 Determination of methanol content Biodiesel analysis - Optimized chromatography supplies Information resources 29-3 Biodiesel analysis guide 3

4 Syringes Inlet liners Ferrules 4

5 Septa The combination of components selected for your instrument make an important contribution to successful separations. Choose Trajan to improve your chromatography. GC columns Biodiesel analysis guide 5

6 Trajan consumables GC selection Syringes SGE syringes for autosamplers incorporate a vibrant color scheme, distinguished by volume, enabling easy identification of syringes installed in instruments. Choose from a comprehensive range of SGE syringe options including plunger protection, removable or fixed s, a range of gauge and length options as well as tip style alternatives. Color Syringe volumes Yellow 1 nl (1 µl) 1 ml 1 ml (1 L) Lime 5 nl (5 µl) 5 µl 5 ml Dark orange 1 µl 1 ml Green 25 µl 25 ml Purple 5 µl 5 ml Aqua 1 µl 1 ml Gray 25 µl 2.5 ml 2 ml (2 L) Light orange 5 nl (.5 µl) 5 µl 5 ml (.5 L) Septa The role of septa for GC analysis is key as many chromatographic problems are caused by use of inappropriate septa material or inappropriate handling of the septa. Desired septa attributes: Reliably seal against the carrier gas pressure in the inlet. Capable of being pierced and resealed time after time. Must not contaminate or bleed material into the chromatographic system. Material Max operating temperature Key features GP grade 2 C Low temperature applications EC grade 35 C, 3 C for 17 mm Low bleed MN grade 35 C, 3 C for 17 mm Premium septa for autosamplers HT grade 4 C, 33 C for 17 mm Outstanding mechanical properties for the highest temperature applications 6

7 Inlet liners The inlet liner is where the sample is introduced and vaporized into the gaseous phase. The design of the liner is crucial, as is liner deactivation, to ensure reproducible and accurate chromatography. To optimize your results for different sample types, SGE Inlet liners are color coded by geometry for ease of selection. Color Injection technique Sample types Liner geometry Dark Green Splitless Trace level analyses. Active compounds. Taper / Gooseneck Blue Split General purpose. Concentrated samples. Dirty samples. Aqua Splitless Trace level analyses. Dirty samples. Wide boiling point range. Orange Direct Trace level analyses. Active compounds. FocusLiner Tapered FocusLiner ConnecTite Purple Split Splitless General purpose. Concentrated samples. Dirty samples (only if quartz wool is present). Gaseous samples (also Purge and Trap, Headspace). Straight Yellow Gray Splitless LVI PTV LVI Trace level analyses. Low boiling point compounds. Active compounds. Trace level analyses. Large volume injections. Double Taper PTV/LVI Connectors and ferrules GC connections are designed to minimize time spent on installation, and are suitable for a wide range of applications. Poorly defined or selected connections can lead to an increase in dead volume, leaks and mismatched tubing s after temperature cycling. Material Uses Advantages Limitations 1% FID, NPD, high temperature Graphite 85% Vespel / 15% Graphite MS and oxygen-sensitive detectors Easy-to-use stable seal Higher temperature limit Can be easily removed Can be re-used Long lifetime High temperature limit MS compatible Siltite Metal MS and oxygen-sensitive detectors Long lifetime High temperature limit MS compatible Not for MS or oxygen-sensitive detectors Soft, easily deformed or destroyed Possible system contamination Cannot be re-used Must be re-tightened after initial temperature cycles Cannot be re-used Biodiesel analysis guide 7

8 Sample preparation Biodiesel requires quantitative measurement of the composition of the sample. Consequently liquid handling accuracy and precision are critical to the success of the final application. evol, the world s first digitally controlled analytical syringe and 21 R&D1 award winner, provides the accuracy and precision required in biodiesel analytical laboratories. evol XR makes everyone an expert Speed up and simplify laboratory workflow Improve accuracy and reproducibility Standardize results independent of operator skill evol is the coupling of two precision devices: a digitally controlled electronic drive and an XCHANGE enabled analytical syringe. XCHANGE syringes are easily and quickly changed allowing them to be dedicated to individual liquids or methods to prevent possible cross-contamination of reagents. evol is ergonomic, comfortable and easy to use. evol is easily calibrated and calibration factors saved for each syringe, enabling laboratories to comply with stringent global laboratory standards. evol is suitable for use with volatile samples. evol is programmable to store a laboratory workflow (up to 98 steps). evol is suitable for direct injection onto a chromatography column with a consistent flow rate. evol s stainless steel enables direct injection through septa. Now available! evol XR 1 ml syringe Password protection options enabling standardization of work processes. Slow aspirate and dispense for titrations and reaction rate control. 1 ml syringe provides more flexibility to improve workflows. evol Electronic Syringe starter kit contains: Description Part number evol XR kit contains: evol syringes 5 μl, 1 μl and 1 ml - Stand - Universal charger - Comprehensive instruction manual - Disc with manual in multiple languages evol only

9 BPX-BIOD GC SGE GC columns Each ASTM and EN biodiesel method has been evaluated to demonstrate the suitability of BPX-BIOD GC columns to achieve optimal separation. Each BPX-BIOD GC column is tested at the maximum operating temperature for the column, and specific tests are undertaken to ensure the column meets inertness specifications and separation performance. This means you can be confident of a reliable separation, column after column. Please refer to the table below and method library on the following pages (6-14) to find the best high temperature column for your biodiesel analysis. Method Description Column ID Length (m) Film thickness (μm) Part number ASTM D6584* BPX BIOD SG53 ASTM D6584* BPX BIOD SG53 EN1413* BPX BIOD EN1415* BPX BIOD SG53 EN1411* BPX BIOD EN1411** BPX - BIOD Wax EN1411** BPX - BIOD Wax *Recommended column in method. **Alternative column recommended in method. Includes guard column. Biodiesel analysis guide 9

10 Method library ASTM D6584 / EN 1415 free and total glycerine Column part number Phase Column 54118SG53 BIOD-BPX6584 or BPX-BIOD5 1 m x.32 mm ID x.1 μm with 2 m x.53 mm ID guard column Initial temperature 5 C, 1 min Rate 1 15 C/min to 18 C Rate 2 7 C/min to 23 C Rate 3 7 C/min Final temperature 38 C, 1 min Detector FID at 4 C Carrier gas He Carrier gas flow 3 ml/min Low level ASTMD6584 / EN1415 standard Glycerol Butanetriol (IS) Monopalmitin Monolein Tricaprin (IS) Diolein 2.13 Triolein Minutes High level ASTMD6584 / EN1415 standard Glycerol Butanetriol (IS) Monopalmitin Monolein Tricaprin (IS) Diolein Triolein Minutes 1

11 Minutes Good yellow grease biodiesel and bad yellow grease biodiesel compared with ASTMD6584 / EN1415 standard Glycerol Butanetriol (IS) Monopalmitin Monolein Tricaprin (IS) Diolein Triolein Good Yellow yellow Grease grease Bad Yellow yellow Grease grease Standard Minutes Soy derived biodiesel sample compared to ASTMD6584 / EN1415 standard Glycerol Butanetriol (IS) Monopalmitin Monolein Tricaprin (IS) Diolein Triolein Soy derived biodiesel Standard Minutes Biodiesel analysis guide 11

12 Method library EN1413 determination of ester and linolenic acid methyl ester contents Column part number Phase BPX-BIOD2 Column 3 m x.32 mm ID x.25 μm Initial temperature 21 C, 9 min Rate 2 C/min Final temperature Carrier gas Carrier gas flow Injection volume 24 C, 6 min He 1.5 ml / min 1 μl RT # Components Wt% C14: (Myristic) C14:1 (Myristoleic) C15: (Pentadecanoic) C15:1 (cis-1-pentadecenoic) C16: (Palmitic) C16:1 (Palmitoleic) C17: (Heptadecanoic) C17:1 (cis-1-heptadecenoic) C18: (Stearic) C18:1n9c (Oleic) C18:1n9t (Elaidic) C18:2n6c (Linoleic) C18:2n6t (Linolelaidic) C18:3n6 (γ-linolenic) C18:3n3 (α-linolenic) C2: (Arachidic) C2:1n9 (cis-11-eicosenoic) C2:2 (cis-11, 14-Eicosadienoic) C2:3n6 (cis-8,11, 14-Eicosatrienoic) C21: (Henicosanoic) C2:3n3 (cis-11,14, 17-Eicosatrienoic) C2:4n6 (Arachidonic) C2:5n3 (cis-5,8,11,14 17-Eicosapentaenoic) C22: (Behenic) C22:1n9 (Erucic) C22:2 (cis-13, 16-Docosadienoic) C23: (Tricosanoic) C24: (Lignoceric) C22:6n3 (cis-4,7,1,13,16, 19-Docosahexaenoic) C24:1n9 (Nervonic) , , , , Minutes 12

13 FAME profile of IQAEP B214 yellow grease C17: C18:1 24 C18: C18: C16: C14: C18:3 4 2 C2: C2:1 C22: C24: Minutes FAME profile of good yellow grease C17: C18: C18: C18: 14 C16: C18:3 4 C14: 4 2 C2: C2:1 C22: C24: Minutes Biodiesel analysis guide 13

14 Method library EN1413 continued FAME profile of bad yellow grease C17: C18: C18: C16: 12 C18: C14: 4 C18:3 2 C2: C2:1 C22: C24: Minutes FAME profile of canola C18:1 24 C17: C18: C16: C18: C18: 4 2 C14: C2:1 C2: C22: C24: Minutes 14

15 FAME profile of sunflower C17: C18: C18: C16: 12 C18: C14: 4 C18:3 2 C2: C2:1 C22: C24: Minutes FAME profile of soy / tallow C17: C18: C18: C16: 12 C18: C14: 4 C18:3 2 C2: C2:1 C22: C24: Minutes Biodiesel analysis guide 15

16 Method library EN1411 methanol content in biodiesel Column part number Phase BPX-BIOD1 Column 3 m x.32 mm ID x 3. µm Initial temperature 21 C, 6 min Rate 2 C/min Final temperature 24 C, 6 min Detector 25 C Carrier gas He Carrier gas flow 1.5 ml/min Injection volume 1 μl Methanol content using EN1411 recommended column - 2. ml/min flow - 5 C - 2 μl injection Methanol 2.3 min Minutes Methanol 2.3 min Propanol 3.7 min Calibration solution A - 25 μl of.5% standard on BP1 column with 5 μl of IPA / 5 g of sample Propanol 3.7 min Minutes 16

17 Calibration solution B - 25 μl of.1% standard on BP1 column with 5 μl of IPA / 5 g of sample Methanol 2.3 min Propanol 3.7 min Minutes Calibration Solution C - 25 μl of.1% standard on BP1 column with 5 μl of IPA / 5 g of sample Propanol 3.7 min Methanol 2.3 min Minutes Biodiesel analysis guide 17

18 Biodiesel analysis Optimized chromatography supplies SGE syringes 1-5 μl NanoVolume Specifications Accuracy and reproducibility Borosilicate glass barrel outer diameter (OD) Scale length International standards traceability ± 2 % (dispensed volume) 6.5 mm and 8 mm.5 µl (6.5 mm OD barrel) = 27.5 mm,.5 µl (8 mm OD barrel) = 63.7 mm, 1 µl = 54.1 mm, 63.7 mm, 5 µl = 48.7 mm Syringe volume Needle length (mm) Needle gauge Needle OD (mm) 8. mm outer diameter (OD) barrel Needle ID (mm) Needle tip 1 µl Cone 1 µl Bevel 1 µl Cone 1 µl Bevel 1 µl Cone 1 µl Cone 5 µl Cone 5 µl Bevel 5 µl Cone 5 µl Bevel 5 µl Cone 5 µl Nozzle Description 1. μl NanoVolume syringe with 5 cm.63 mm OD cone tipped 1. μl NanoVolume syringe with 5 cm.63 mm OD bevel tipped 1. μl NanoVolume syringe with 7 cm.63 mm OD cone tipped 1. μl NanoVolume syringe with 7 cm.63 mm OD bevel tipped 1. μl NanoVolume syringe with 11.5 cm.63 mm OD bevel tipped 1. μl NanoVolume syringe with 7 cm.47 mm OD cone tipped 5. μl NanoVolume syringe with 5 cm.63 mm OD cone tipped 5. μl NanoVolume syringe with 5 cm.63 mm OD bevel tipped 5. μl NanoVolume syringe with 7 cm.63 mm OD cone tipped 5. μl NanoVolume syringe with 7 cm.63 mm OD bevel tipped 5. μl NanoVolume syringe with 11.5 cm.63 mm OD cone tipped 5. μl NanoVolume syringe with 7 cm.63 mm OD nozzle tipped Replacement and plunger Syringe Syringes fitted with repeating adaptor The SGE repeating adaptor (RAX) is attached to a syringe to ensure reproducibility of sample volumes. Syringe volume Needle length (mm) Needle gauge Needle OD (mm) 8. mm outer diameter (OD) barrel Needle ID (mm) Needle tip 1 µl Cone 1 µl Cone Description 1. μl NanoVolume syringe with 5 cm.63 mm OD cone tipped and repeating adaptor 1. μl NanoVolume syringe with 7 cm.63 mm OD cone tipped and repeating adaptor Replacement and plunger Syringe

19 Manual and on-column syringes 1-5 μl PTFE tipped plunger Specifications Accuracy and reproducibility Borosilicate glass barrel outer diameter (OD) Scale length International standards traceability ± 1 % (dispensed volume) 1 µl = 6.5 mm, 25 5 µl = 8 mm 1 µl = 54.1 mm, 25 5 µl = 6 mm Syringe volume Fixed PTFE tipped plunger Needle length (mm) Needle gauge Needle OD (mm) Needle ID (mm) Needle tip 1 µl P Bevel 1 µl P Bevel 25 µl P Bevel 5 µl P Bevel 1 µl P Bevel 25 µl P Bevel 5 µl P Bevel Removable 1 µl P Bevel 25 µl P Bevel 5 µl P Bevel 1 µl P Bevel 25 µl P Bevel 5 µl P Bevel Guided plunger 1 µl P Bevel On-column 1 µl P Oncolumn Description 1 μl fixed syringe with GT plunger and 5 cm.47 mm OD bevel tipped 1 μl fixed syringe with GT plunger and 7 cm.47 mm OD bevel tipped 25 μl fixed syringe with GT plunger and 5 cm.5 mm OD bevel tipped 5 μl fixed syringe with GT Plunger & 5 cm.5 mm OD bevel tipped 1 μl fixed syringe with GT plunger and 5 cm.5 mm OD bevel tipped 25 μl fixed syringe with GT plunger and 5 cm.5 mm OD bevel tipped 5 μl fixed syringe with GT plunger and 5 cm.5 mm OD bevel tipped 1 μl removable syringe with GT plunger and 5 cm.47 mm OD bevel tipped 25 μl removable syringe with GT plunger and 5 cm.5 mm OD bevel tipped 5 μl removable syringe with GT plunger and 5 cm.5 mm OD bevel tipped 1 μl removable syringe with GT plunger and 5 cm.5 mm OD bevel tipped 25 μl removable syringe with GT plunger and 5 cm.5 mm OD bevel tipped 5 μl removable syringe with GT plunger and 5 cm.5 mm OD bevel tipped 1 μl removable guided plunger syringe with GT plunger and 5 cm.47 mm OD bevel tipped Replacement Replacement plunger 6 PK syringe Syringe R-GT-OCCE Biodiesel analysis guide 19

20 Agilent Technologies GC supplies SGE autosampler syringes All s are 42 mm long with a cone tip. Agilent 7673, 7683, 7693A and 685 ALS Volume Needle gauge (OD mm) Fixed tapered 5 µl 23-26s (.63/.47) 1 µl 23-26s (.63/.47) 1 µl 23-26s (.63/.47) gas tight Fixed straight Description 5 μl fixed Agilent syringe with 4.2 cm.63/.47 mm OD cone tipped dual gauge 1 μl fixed Agilent syringe with 4.2 cm.63/.47 mm OD cone tipped dual gauge 1 μl fixed Agilent syringe with GT plunger and 4.2 cm.63/.47 mm OD cone tipped dual gauge Syringe Spare Spare plunger µl 23 (.63) 5 μl fixed Agilent syringe with 4.2 cm.63 mm OD cone tipped µl 23 (.63) 1 μl fixed Agilent syringe with 4.2 cm.63 mm OD cone tipped Removable tapered 5 µl 23-26s (.63/.47) 5R-AG-.63/.47C µl 23-26s (.63/.47) 1R-AG-.63/.47C Removable striaght 1 µl 23 (.63) 1BR-AG-.63C ** ** Denotes plunger and kit. Septa Instrument Diameter Septa type Agilent Technologies 789, 689, 589, 588, 489 and 685 Part no. 11 HT EC SGE Inlet liners Description and geometry sketch OD (mm) ID (mm) Length (mm) For Agilent 589, 685, 689, 789 and HP489 Split/splitless FocusLiner Split, straight-through liner Part no The SGE Inlet liner range is color coded by geometry to simplify selection. FocusLiner Straight 2

21 Ferrules Instrument Column ID Ferrule ID Part no. 15% graphite / 85% Vespel ferrules mm.4 mm mm.5 mm mm.8 mm For injectors and detectors at for 1/8" OD packed columns 1/8" atmospheric pressure e.g. FID for 1/4" OD packed columns 1/4" mm.4 mm mm.5 mm mm.8 mm SilTite metal ferrules mm.4 mm 1 *732 For GC-MS interface connection (starter kit).32 mm.5 mm 1 * mm.8 mm 1 * mm.4 mm 1 #7327 For split/splitless injectors.32 mm.5 mm 1 #73271 (starter kit) mm.8 mm 1 # /32".81 mm 1 #73273 Replacement SilTite metal ferrules For all connections mm.4 mm mm.5 mm mm.8 mm /32".81 mm Replacement SilTite nuts For GC-MS interface connection For split/splitless injector Replacement SilTite base seals For split/splitless injector * Includes 1 ferrules, 2 SilTite nuts. # Includes 1 ferrules, 2 SilTite nuts and 2 SilTite inlet base seals. Biodiesel analysis guide 21

22 PerkinElmer GC supplies SGE autosampler syringes All s are 7 mm long with a cone tip style. PerkinElmer AutoSystem Volume Fixed Needle gauge (OD mm) 5 µl 26 (.47) 5 µl 23 (.63) 5 µl gas tight 5 µl gas tight 26 (.47) 23 (.63) 5 µl 23 (.63) Description 5 μl fixed PerkinElmer syringe with 7 cm.47 mm OD cone tipped 5 μl fixed PerkinElmer syringe with 7 cm.63 mm OD cone tipped.5 μl NanoVolume Agilent Syringe with 4.2 cm.47 mm OD cone tipped 5 μl fixed PerkinElmer syringe with GT plunger and 7 cm.63 mm OD cone tipped 5 μl fixed PerkinElmer syringe with 7 cm.63 mm OD cone tipped Syringe Spare plunger Septa Instrument Diameter Septa type PerkinElmer Sigma, 9, 99, 8 series, AutoSystem and Clarus Part no. 11 HT HT SGE Inlet liners Description and geometry sketch OD (mm) ID (mm) Length (mm) For PerkinElmer AutoSystem and Clarus 5, 6 Split/splitless FocusLiner Split, straight-through liner Part no The SGE Inlet liner range is color coded by geometry to simplify selection. FocusLiner Straight 22

23 Ferrules Instrument Column ID Size of nut Ferrule ID Part no. 15% graphite / 85% Vespel ferrules mm 1/16".4 mm mm 1/8".4 mm mm 1/16".5 mm For injectors and detectors at.32 mm 1/8".5 mm atmospheric pressure e.g. FID mm 1/16".8 mm mm 1/8".8 mm /8 OD packed columns 1/8" 1/8" /4 OD packed columns 1/4" 1/4" SilTite metal ferrules mm.4 mm 1* 732 For GC-MS interface connection (starter kit).32 mm.5 mm 1* mm.8 mm 1* 7322 Replacement SilTite ferrules mm.4 mm For GC-MS interface.32 mm.5 mm connection.53 mm.8 mm /32".81 mm Replacement SilTite nuts For GC-MS interface connection For split/splitless injector *Includes 1 ferrules, 2 SilTite nuts. Biodiesel analysis guide 23

24 Shimadzu GC supplies SGE autosampler syringes All s are 42 mm long with a cone tip style. Shimadzu AOC6 Volume Needle gauge (OD mm) Description Syringe Spare Spare plunger Fixed 5 µl 26 (.47) 5F C µl 26 (.47) 1F C µl gas tight 26 (.47) 1F-GT C µl gas tight 26 (.47) 25F-GT C µl gas tight 26 (.47) 1F-GT C Removable 1µL 23 (.63) 1BR C * * 1 * Replacement and plunger is supplied as a set. Septa Instrument Shimadzu 9A, 14, 15A, 16, 17A, GC-21, GC-214 and GC-23 Septa type Part no. HT EC SGE Inlet liners Description and geometry sketch OD (mm) ID (mm) Length (mm) Part no. For GC-23 (SPL-23 injector), GC-21 (SPL-21 injector), GC-214 (SPL-214 injector) and GC-17A (SPL-17 injector) Split/splitless FocusLiner * Split/splitless FocusLiner * Split, straight-through liner * * When using a standard 42 mm for autosamplers, the sample will be injected on top of the wool for this liner. The SGE Inlet liner range is color coded by geometry to simplify selection. FocusLiner Straight 24

25 O-rings and sealing rings Description Usage Part no. Graphite sealing ring Can be used at temperatures up to 45 C. For 14, 15A and 16 (SPL-14 injector) Graphite sealing ring Can be used at temperatures up to 45 C. For 17A (SPL-17 injector) Viton o-ring Can be used at temperatures up to 3 C. For GC-21 and GC-214 (SPL-21 injector and SPL-214 injector) Ferrules Column ID Description Part no. GC14A, GC17A, GC-21, GC-214 and GC-23 detectors/injectors (not for MS interfaces or QP21 injector) mm ID columns SilTite metal - initial installation 1* mm ID columns SilTite ferrules mm ID columns SilTite metal - initial installation 1* mm ID columns SilTite ferrules mm ID columns SilTite metal - initial installation 1* mm ID columns SilTite ferrules n/a SilTite metal nuts - slotted QP5/55 standard MS interface QP5-I mm ID columns 15% Graphite/ 85% Vespel ferrules QP5-I.32 mm ID columns 15% Graphite/ 85% Vespel ferrules QP5-II and QP mm ID columns 15% Graphite/ 85% Vespel ferrules QP5-II and QP55.32 mm ID columns 15% Graphite/ 85% Vespel ferrules mm ID columns SilTite metal - initial installation 1* mm ID columns SilTite ferrules mm ID columns SilTite metal - initial installation 1* mm ID columns SilTite ferrules mm ID columns SilTite metal - initial installation 1* mm ID columns SilTite ferrules QP5/55 wide bore MS interface, QP21 injector and QP21 standard MS interface mm ID columns 15% Graphite/ 85% Vespel ferrules mm ID columns 15% Graphite/ 85% Vespel ferrules mm ID columns 15% Graphite/ 85% Vespel ferrules mm ID columns SilTite metal - initial installation 1* mm ID columns SilTite ferrules mm ID columns SilTite metal - initial installation 1* mm ID columns SilTite ferrules mm ID columns SilTite metal - initial installation 1* mm ID columns SilTite ferrules n/a SilTite metal nuts * Includes 1 ferrules, 2 SilTite nuts. Replacement parts Material Part no. Replacement SilTite nuts For GC-21 and GC-23 GC-MS system For GC-21 and GC-23 GC-MS system with QP5 series MS For GC-21/GC-23/GC-214 GC injectors and atmospheric detectors QP5 jet separator MS interface QP5 direct MS interface For all injectors jet separator (starter kit), except GC-21/GC-23/GC Biodiesel analysis guide 25

26 Thermo Scientific GC supplies SGE autosampler syringes All s have a cone tip style. Thermo Scientific TriPlus RSH Volume Removable Needle gauge (OD mm) 5 µl 23 (.47) 57 5 µl 26 (.47) 57 1 µl 23 (.47) 57 1 µl 26 (.47) 57 Fixed 1 µl 23 (.63) 57 1 µl gas tight 23 (.63) 57 1 µl 23 (.63) 85 1 µl gas tight 23 (.63) 85 1 µl 26 (.47) 57 1 µl gas tight 26 (.47) 57 1 µl 26 (.47) 85 1 µl gas tight 26 (.47) 85 Needle length (mm) Description 5 μl removable CTC RTC and Thermo RSH syringe with 5.7 cm.63 mm OD cone tipped 5 μl removable CTC RTC and Thermo RSH syringe with 5.7 cm.47 mm OD cone tipped 1 μl removable CTC RTC and Thermo RSH syringe with 5.7 cm.63 mm OD cone tipped 1 μl removable CTC RTC and Thermo RSH syringe with 5.7 cm.47 mm OD cone tipped 1 μl fixed CTC RTC and Thermo RSH syringe with 5.7 cm.63 mm OD cone tipped 1 μl fixed CTC RTC and Thermo RSH syringe with GT plunger and 5.7 cm.63 mm OD cone tipped 1 μl fixed CTC RTC and Thermo RSH syringe with 8.5 cm.63 mm OD cone tipped 1 μl fixed CTC RTC and Thermo RSH syringe with GT plunger and 8.5 cm.63 mm OD cone tipped 1 μl fixed CTC RTC and Thermo RSH syringe with 5.7 cm.47 mm OD cone tipped 1 μl fixed CTC RTC and Thermo RSH syringe with GT plunger and 5.7 cm.47 mm OD cone tipped 1 μl fixed CTC RTC and Thermo RSH syringe with 8.5 cm.47 mm OD cone tipped 1 μl fixed CTC RTC and Thermo RSH syringe with GT plunger and 8.5 cm.47 mm OD cone tipped Syringe Septa Instrument Diameter Septa type Thermo Scientific TRACE 13 Series GC Part no. 11 HT EC

27 SGE Inlet liners Description and geometry sketch OD (mm) ID (mm) Length (mm) For Thermo Scientific TRACE 13 Series GC Split/splitless FocusLiner Split, straight-through liner Part no The SGE Inlet liner range is color coded by geometry to simplify selection. O-rings and sealing rings FocusLiner Straight Description Usage Part no. For Thermo Scientific TRACE 13 Series GC Viton o-ring Temperatures up to 3 C. Suitable for liners with OD of 6.3 mm Graphite sealing ring Temperatures up to 45 C. Suitable for all inlet liners above except 924 and Graphite sealing ring Temperatures up to 45 C. Suitable for use with liners 924 and SilTite FingerTite ferrules Description Column ID Ferrule ID Part no. For Thermo Scientific TRACE 13 Series GC SilTite FingerTite INJ / FID starter kit mm.4 mm * 7361 SilTite FingerTite capillary / FID starter kit mm.4 mm * SilTite FingerTite INJ / MS starter kit mm.4 mm * Replacement parts SilTite FingerTite ferrule.4 mm mm.4 mm SilTite FingerTite ferrule.5 mm.32 mm.5 mm SilTite FingerTite ferrule.7 mm.53 mm.7 mm SilTite FingerTite ferrule blanking SilTite FingerTite female nut SilTite FingerTite INJ base seal mm SilTite FingerTite MS adaptor SilTite FingerTite injector mm * Each starter kit includes all the parts necessary to convert one GC system (one injector and one detector) to the SilTite FingerTite system. In addition there are five SilTite FingerTite nuts, ten SilTite FingerTite ferrules, and a ferrule install tool which allows you to seat the ferrule in the correct position on the capillary column. Biodiesel analysis guide 27

28 Thermo Scientific GC supplies Ferrules Instrument Column ID Ferrule ID Part no. 15% graphite / 85% Vespel ferrules mm.4 mm mm.5 mm For TRACE 13 injectors and detectors at atmospheric.53 mm.8 mm pressure e.g. FID for 1/8" OD packed columns 1/8" for 1/4" OD packed columns 1/4" mm.4 mm For TRACE 13 GC-MS interface.32 mm.5 mm connection.53 mm.8 mm % graphite ferrules mm.5 mm For TRACE 13 injectors and detectors at atmospheric mm.8 mm pressure e.g. FID (not for GC-MS) for 1/8" OD packed columns 1/8" for 1/4" OD packed columns 1/4" SilTite metal ferrules mm.4 mm 1* 7345 GC-MS interface connection (starter kit).32 mm.5 mm 1* mm.8 mm 1* mm.4 mm For TRACE 13 split/splitless injectors.32 mm.5 mm (starter kit) mm.8 mm /32".81 mm Replacement SilTite nuts SilTite metal nuts Replacement SilTite metal ferrules mm.4 mm For All GC-MS interface.32 mm.5 mm connections.53 mm.8 mm /32".81 mm mm.4 mm For TRACE 13 connections.32 mm.5 mm mm.8 mm /32".81 mm Replacement SilTite nuts SilTite metal nuts For TRACE 13 GC-MS interface connection For TRACE 13 split/splitless injector Replacement SilTite base seals For TRACE 13 split/splitless injector * Includes ten ferrules, two SilTite nuts. # To be used in combination with brass nut ( 13485). 28

29 Information resources For official methodolgy ASTM International ASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. DIN Deutsches Institut fur Normung A range of international standards and reference for EN methods. Industry resources National Biodiesel Board Body responsibly for the development of the US biodiesel industry. Journals BioDiesel Magazine Biodiesel industry journal for news on commercial and technical developments. BioFuels International Industry journal for biofuels that features biodiesel related news. Biodiesel analysis guide 29

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32 Biodiesel Analysis guide This guide has been created as a resource for biodiesel analysis. Inside you will find method information, products and solutions designed to ensure you can effectively and reliably complete fuels testing according to either ASTM Internationally or European National (EN) methods. The latest information about our products and applications can be found at our website: Visit us at or contact your regional Trajan representative for assistance and further information. BR-392-G_RevF Trajan Scientific Australia Pty Ltd 11/217 Trajan Scientific and Medical Science that benefits people Trajan is actively engaged in developing and delivering solutions that have a positive impact on human wellbeing. Our vision revolves around collaborative partnerships that improve workflows, delivering better results.