Method-Specific Sample Preparation for EPH Analysis

Transcription

1 Method-Specific Sample Preparation for EPH Analysis Resprep SPE Cartridges for Complete Extractable Petroleum Hydrocarbon Fractionation Full separation between fractions. Guaranteed low background level. Superior lot-to-lot reproducibility.

2 Resprep EPH Fractionation SPE Cartridges Method-specific performance for extractable petroleum hydrocarbon (EPH) analysis of soil and water samples. Complete separation of aliphatic and aromatic compounds into distinct fractions. Guaranteed background level under the strict reporting limits of MA and NJ EPH methods. Superior lot-to-lot reproducibility and storage stability ensured by rigorous QC testing and moisture-resistant packaging. Resprep EPH fractionation SPE cartridges from Restek are manufactured in a strictly controlled environment with the simple goal of giving you consistent results every day, every time. Turn to Resprep cartridges for easy, reproducible, and guaranteed extractable petroleum hydrocarbon analysis. Order yours today at Figure 1: Resprep EPH SPE cartridges offer consistent and complete separation between aliphatic and aromatic compounds from one lot to the next. A representative subset of compounds is shown here (40+ compounds were analyzed in total) to clearly illustrate the separation between fractions and the lack of breakthrough in every lot we produce. Resprep EPH Fractionation SPE Cartridge, Lot 1 Resprep EPH Fractionation SPE Cartridge, Lot 3 Hexane / Aliphatic Fraction Dichloromethane / Aromatic Fraction Hexane / Aliphatic Fraction Dichloromethane / Aromatic Fraction Elution Volume (ml) Elution Volume (ml) Resprep EPH Fractionation SPE Cartridge, Lot 2 C9 Chlorooctadecane Pyrene Hexane / Aliphatic Fraction Dichloromethane / Aromatic Fraction C20 C30 Trimethylbenzene Naphthalene Dibenz(a,h)anthracene o-terphenyl C40 2-Bromonaphthalene No guesswork, no worries. Detailed, step-by-step instructions and a complete certificate of analysis are included with every 15-pack of Resprep EPH cartridges Elution Volume (ml) 2

3 Table I: Recovery data (n = 3) for Resprep EPH SPE cartridges. Again, 40+ compounds were analyzed; shown here are the compounds most critical to method requirements. Aliphatic Fraction Compound Concentration (ppm) Function Average % Recovery %RSD C9 200 Most-retained aliphatic % Chlorooctadecane 200 Surrogate % C28 4,000 Aliphatic loading capacity probe % C Least-retained aliphatic % Aromatic Fraction Compound Concentration (ppm) Function Average % Recovery %RSD Trimethylbenzene 200 Least-retained aromatic % Naphthalene 200 Breakthrough probe % 2-Methylnaphthalene 200 Breakthrough probe % 2-Fluorobiphenyl 200 Surrogate % 2-Bromonaphthalene 200 Surrogate % Phenanthrene 4,000 Aromatic loading capacity probe % o-terphenyl 200 Surrogate % Dibenzo(a,h)anthracene 200 Most-retained aromatic % Figure 2: Storage stability is enhanced by new, moisture-resistant packaging that prevents ambient moisture from reaching your SPE cartridge and helps ensure outstanding performance. Moisture Content after Storage in 80% Relative Humidity Moisture (%) Storage Time (weeks) 3

4 Figure 3: Restek guarantees a background level in each fraction of less than half the strict reporting limits of MA and NJ EPH methods. EPH Silica Cartridge Background Extraction Aliphatic Fraction on Rxi-5ms Conc. Peaks t R (min) (µg/ml) 1. 1-Chlorooctadecane Region 1 Total extracted background = 8.2 ppm Column Rxi-5ms, 30 m, 0.32 mm ID, 0.25 µm (cat.# 13424) Sample MA EPH surrogate spike mix (cat.# 31479) Conc.: 5 µg/ml Injection Inj. Vol.: 1 µl splitless (hold 1 min) Liner: Restek Premium 4.0 mm ID cyclo double taper inlet liner (cat.# 23310) Inj. Temp.: 300 C Purge Flow: 50 ml/min Oven Oven Temp.: 40 C (hold 1 min) to 300 C at 12 C/min to 330 C at 6 C/min Carrier Gas He, constant flow 1-Chlorooctadecane at 5 ppm Flow Rate.: Linear Velocity: 2 ml/min C Dead Time: C Detector 330 C Make-up Gas Flow Rate: 45 ml/min Make-up Gas Type: N₂ Hydrogen flow: 40 ml/min Air flow: 450 ml/min Data Rate: 50 Hz Instrument Agilent 7890B GC Notes The area of the surrogate peak was excluded from the calculated total extracted background value for Region 2. Region 2 Total extracted background = 11.5 ppm Time (min) GC_EV1437 EPH Silica Cartridge Background Extraction Aromatic Fraction on Rxi-5ms Conc. Peaks t R (min) (µg/ml) 1. o-terphenyl o-terphenyl at 5 ppm Region 1 Total extracted background = 14.2 ppm Region 2 Total extracted background = 13.1 ppm Time (min) GC_EV

5 Product Listing Resprep EPH Fractionation SPE Cartridge Method-specific performance for extractable petroleum hydrocarbon (EPH) analysis of soil and water samples. Complete separation of aliphatic and aromatic compounds into distinct fractions. Guaranteed background level under the strict reporting limits of MA and NJ EPH methods. Superior lot-to-lot reproducibility and storage stability ensured by rigorous QC testing and moisture-resistant packaging. Description Volume qty. cat.# Resprep EPH Fractionation SPE Cartridge 25 ml, 5 g 15-pk No-risk, no-obligation trial. Free samples of Resprep solid phase extraction (SPE) tubes are available. Contact us or your local Restek representative to request yours: Resprep 12- or 24-Port SPE Manifolds Use with any standard male luer end SPE cartridges. Inert, PTFE sample guides reduce cross-contamination and carryover. Flexible sample collection rack will accommodate a variety of receiving vessels. Quick vacuum-release valve for better system control. Individual valves allow vacuum control for each cartridge, improving reproducibility. Description qty. cat.# Complete Resprep SPE Manifold, 12-Port (Includes: glass basin with built-in vacuum regulator (1); polypropylene lid with 12 individual control valves (1); 12-position collection rack (1); PTFE sample guides (12); and kit waste container (1).) Complete Resprep SPE Manifold, 24-Port (Includes: glass basin with built-in vacuum regulator (1); polypropylene lid with 24 individual control valves (1); 24-position collection rack (1); and PTFE sample guides (24).) kit Resprep Manifold Replacement Parts Description qty. cat.# Replacement Waste Container, 12-Port ea Replacement Vacuum Valve and Gauge Assembly ea Glass Vacuum Chamber w/gauge & valve for Resprep manifolds, 6 or 12-Port ea Collection Rack Collection Rack, 12-Port ea Collection Rack, 24-Port ea Manifold Lid Replacement Manifold Lid (sample guides not included), 12-Port ea Replacement Manifold Lid (sample guides not included), 24-Port ea Manifold Lid Replacement Parts Valves, 12 or 24-Port 2-pk Valve Attachment 48-pk Needle Attachment 48-pk Sample Guide Needles, 12 or 24-Port 12-pk NOTE: Replacement waste container not included in 24-port manifold kit (cat.# 26080). *Waste container (shown in tank) and top shelf for round-bottom flasks are not included in 24-port manifold kit (cat.# 26080). Resprep 12-Port SPE Manifold 5

6 Rxi-5ms Columns (fused silica) low-polarity phase; Crossbond diphenyl dimethyl polysiloxane similar phases HP-5ms SemiVolatiles, HP-5ms, HP-5msUI, DB-5, Ultra-2, CP-Sil 8 CB, ZB-5, ZB-5msi General-purpose columns for semivolatiles, phenols, amines, residual solvents, drugs of abuse, pesticides, PCB congeners (e.g., Aroclor mixes), solvent impurities. Most inert column on the market. Tested and guaranteed for ultra-low bleed; improved signal-to-noise ratio for better sensitivity and mass spectral integrity. Temperature range: -60 C to 330/350 C. Equivalent to USP G27 and G36 phases. Description temp. limits qty. cat.# 30 m, 0.32 mm ID, 0.25 µm -60 to 330/350 C ea Si 5% O CH 3 Si CH 3 95% O 2.0 ml, 9 mm Short-Cap, Screw-Thread Vials, 12 x 32 mm Fit all 2.0 ml, 12 x 32 mm, crimp-top vial-based autosamplers. 2.0 ml, 9 mm Short-Cap, Screw-Thread Vials (vial only) Ideal for Agilent 7673, 7683, 7693 & other autosamplers that process 12 x 32 mm crimp-top vials. Description Color 100-pk. 1,000-pk. Short-Cap Vial w/white Graduated Marking Spot Amber ml, 9 mm Short-Cap, Screw-Vial Closures (Polypropylene, preassembled) Cap Color Septa Material Type 100-pk. 1,000-pk. Blue PTFE/Silicone Screw-Thread Choose preslit caps (available for some vials) to reduce the risk of needle bending, release vacuum from high-volume injections, and improve injection reproducibility when greater than 20% of vial volume is withdrawn. 2.0 ml, 9 mm Screw-Thread Vial Convenience Kits (Vials, Caps, & Septa) Vials packaged in a clear-lid tray. Caps with septa packaged in a plastic bag. 100-pk. 1,000-pk. Description cat.# cat.# Amber Vial w/graduated Marking Spot, 9 mm, Blue Cap PTFE/Silicone Liner Crimp-top and other vials also available! Find your vial at 6

7 NJDEP EPH 10/08 Rev. 2 Aliphatics Calibration Standard (20 components) (C9) n-nonane ( ) (C10) n-decane ( ) (C12) n-dodecane ( ) (C14) n-tetradecane ( ) (C16) n-hexadecane ( ) (C18) n-octadecane ( ) (C20) n-eicosane ( ) (C21) n-heneicosane ( ) (C22) n-docosane ( ) (C24) n-tetracosane ( ) (C26) n-hexacosane ( ) (C28) n-octacosane ( ) (C30) n-triacontane ( ) (C32) n-dotriacontane ( ) (C34) n-tetratriacontane ( ) (C36) n-hexatriacontane ( ) (C38) n-octatriacontane ( ) (C40) n-tetracontane ( ) 2-Methylnaphthalene ( ) Naphthalene ( ) 2,000 µg/ml each in hexane:carbon disulfide (80:20), 1 ml/ampul cat.# (ea.) NJDEP EPH 10/08 Rev. 2 Aromatics Calibration Standard (18 components) Acenaphthene ( ) Acenaphthylene ( ) Anthracene ( ) Benz(a)anthracene ( ) Benzo(a)pyrene ( ) Benzo(b)fluoranthene ( ) Benzo(ghi)perylene ( ) Benzo(k)fluoranthene ( ) Chrysene ( ) Dibenz(a,h)anthracene ( ) Fluoranthene ( ) Fluorene ( ) Indeno(1,2,3-cd)pyrene ( ) 2-Methylnaphthalene ( ) Naphthalene ( ) Phenanthrene ( ) Pyrene ( ) 1,2,3-Trimethylbenzene ( ) 2,000 µg/ml each in methylene chloride, 1 ml/ampul cat.# (ea.) NJDEP EPH 10/08 Rev. 2 Aliphatics Matrix Spike Mix (18 components) (C9) n-nonane ( ) (C10) n-decane ( ) (C12) n-dodecane ( ) (C14) n-tetradecane ( ) (C16) n-hexadecane ( ) (C18) n-octadecane ( ) (C20) n-eicosane ( ) (C21) n-heneicosane ( ) (C22) n-docosane ( ) (C24) n-tetracosane ( ) (C26) n-hexacosane ( ) (C28) n-octacosane ( ) (C30) n-triacontane ( ) (C32) n-dotriacontane ( ) (C34) n-tetratriacontane ( ) (C36) n-hexatriacontane ( ) (C38) n-octatriacontane ( ) (C40) n-tetracontane ( ) 200 µg/ml each in pentane, 5 ml/ampul cat.# (ea.) NJDEP EPH 10/08 Rev. 2 Aromatics Matrix Spike Mix (18 components) Acenaphthene ( ) Acenaphthylene ( ) Anthracene ( ) Benz(a)anthracene ( ) Benzo(a)pyrene ( ) Benzo(b)fluoranthene ( ) Benzo(ghi)perylene ( ) Benzo(k)fluoranthene ( ) Chrysene ( ) Dibenz(a,h)anthracene ( ) Fluoranthene ( ) Fluorene ( ) Indeno(1,2,3-cd)pyrene ( ) 2-Methylnaphthalene ( ) Naphthalene ( ) Phenanthrene ( ) Pyrene ( ) 1,2,3-Trimethylbenzene ( ) 200 µg/ml each in acetone:toluene (50:50), 5 ml/ampul cat.# (ea.) NJDEP EPH 10/08 Rev. 2 Aromatics Fractionation Check Mix (16 components) Acenaphthene ( ) Acenaphthylene ( ) Anthracene ( ) Benz(a)anthracene ( ) Benzo(a)pyrene ( ) Benzo(b)fluoranthene ( ) Benzo(ghi)perylene ( ) Benzo(k)fluoranthene ( ) MA EPH Aromatic Hydrocarbon Standard (17 components) Acenaphthene ( ) Acenaphthylene ( ) Anthracene ( ) Benz(a)anthracene ( ) Benzo(a)pyrene ( ) Benzo(b)fluoranthene ( ) Benzo(k)fluoranthene ( ) Benzo(ghi)perylene ( ) Chrysene ( ) Dibenz(a,h)anthracene ( ) Fluoranthene ( ) Fluorene ( ) Indeno(1,2,3-cd)pyrene ( ) 2-Methylnaphthalene ( ) Naphthalene ( ) Phenanthrene ( ) Pyrene ( ) 1,000 µg/ml each in methylene chloride, 1 ml/ampul cat.# (ea.) MA EPH Aliphatic Hydrocarbon Standard (14 components) (C9) n-nonane ( ) (C10) n-decane ( ) (C12) n-dodecane ( ) (C14) n-tetradecane ( ) (C16) n-hexadecane ( ) (C18) n-octadecane ( ) (C19) n-nonadecane ( ) Chrysene ( ) Dibenz(a,h)anthracene ( ) Fluoranthene ( ) Fluorene ( ) Indeno(1,2,3-cd)pyrene ( ) Phenanthrene ( ) Pyrene ( ) 1,2,3-Trimethylbenzene ( ) 400 µg/ml each in hexane:toluene (50:50), 5 ml/ampul cat.# (ea.) MA EPH Surrogate Spike Mix (2 components) 1-Chlorooctadecane ( ) o-terphenyl ( ) 4,000 µg/ml each in acetone, 1 ml/ampul cat.# (ea.) (C20) n-eicosane ( ) (C22) n-docosane ( ) (C24) n-tetracosane ( ) (C26) n-hexacosane ( ) (C28) n-octacosane ( ) (C30) n-triacontane ( ) (C36) n-hexatriacontane ( ) 1,000 µg/ml each in hexane, 1 ml/ampul cat.# (ea.) NJDEP EPH 10/08 Rev. 2 Aliphatics Fractionation Check Mix (18 components) (C9) n-nonane ( ) (C10) n-decane ( ) (C12) n-dodecane ( ) (C14) n-tetradecane ( ) (C16) n-hexadecane ( ) (C18) n-octadecane ( ) (C20) n-eicosane ( ) (C21) n-heneicosane ( ) (C22) n-docosane ( ) (C24) n-tetracosane ( ) (C26) n-hexacosane ( ) (C28) n-octacosane ( ) (C30) n-triacontane ( ) (C32) n-dotriacontane ( ) (C34) n-tetratriacontane ( ) (C36) n-hexatriacontane ( ) (C38) n-octatriacontane ( ) (C40) n-tetracontane ( ) 400 µg/ml each in hexane, 5 ml/ampul cat.# (ea.) Learn more about Restek reference standards at 7

8 Solid Phase Extraction Cartridges Proven Quality Superior Cleanliness Method-Specific Performance Available with the following sorbents: Silica: Multipurpose EPH Silica: Petroleum Florisil Adsorbent: Pesticides CarboPrep Adsorbent: Dirty Samples FREE samples available! Contact us or your local Restek representative. Learn more at Questions about this or any other Restek product? Contact us or your local Restek representative ( Restek patents and trademarks are the property of Restek Corporation. (See for full list.) Other trademarks in Restek literature or on its website are the property of their respective owners. Restek registered trademarks are registered in the U.S. and may also be registered in other countries Restek Corporation. All rights reserved. Printed in the U.S.A. Lit. Cat.# EVSS2574-UNV