Vivacon 2 Ultrafiltration devices desalting, concentrating

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Vivacon 2 Ultrafiltration devices desalting, concentrating Reproducible DNA sample desalting and concentration Vivacon 2 centrifugal concentrators offer the optimal solution for DNA concentration and

High recoveries and excellent reproducibilities are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.

1 Vivacon 2 Ultrafiltration devices desalting, concentrating Reproducible DNA sample desalting and concentration Vivacon 2 centrifugal concentrators offer the optimal solution for DNA concentration and buffer exchange applications. For optimal performance with very dilute samples, e. g. forensic samples, Vivacon 2 is equipped with the patented regenerated cellulose membrane Hydrosart. High recoveries and excellent reproducibilities are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices. The possibility of a re-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations. Feature Re-spin possibility Low binding material Easy to remove re-spin cap Graduation printed on Benefit Complete and highly reproducible sample recovery High recoveries of low sample recovery Convenient sample handling Optimal process control Conversion Table for Hydrosart MWCO to Nucleotide Cut-off Membrane: Hydrosart Membrane Double-Stranded MWCO Nucleotide Cut-off (bp) Performance Characteristics MWCO (Da) Sample size (bp) Time to concentrate up to 30x [min.] at 20 C Concentrate recovery % g-force (x g) 2 kda > min 92 % kda > min 94 % kda > min 95 % kda > min 96 % kda > min 93 % Ordering Information Vivacon 2 devices Pack size Prod. No. Vivacon 2 Accessories Prod. No MWCO 25 VN02H91 Vivacon 2 Rack VNA MWCO 100 VN02H MWCO 500 VN02H MWCO 25 VN02H MWCO 100 VN02H MWCO 500 VN02H MWCO 25 VN02H MWCO 100 VN02H MWCO 500 VN02H MWCO 25 VN02H MWCO 100 VN02H MWCO 500 VN02H MWCO 25 VN02H MWCO 100 VN02H MWCO 500 VN02H43

VivaSpin Ultrafiltration devices desalting, concentrating Reproducible sample desalting and concentration Vivaspin 500: for 100 to 600 µl samples Vivaspin 500 µl centrifugal filter units offer a

The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations, even with particle laden solutions.

This device combines the speed of the classic Vivaspin products with low internal surface and membrane area for superior recoveries from very dilute solutions.

Also unique to the Vivaspin 2, is the choice of directly pipetting the concentrate from the dead stop pocket built into the bottom of the concentrator, or alternatively reverse spinning into the

Vivaspin 4: for 1-4 ml samples Vivaspin 4 ml concentrators are disposable ultrafiltration devices for the concentration of biological samples. Maximum initial sample volumes range from 1 ml to 4 ml.

2 VivaSpin Ultrafiltration devices desalting, concentrating Reproducible sample desalting and concentration Vivaspin 500: for 100 to 600 µl samples Vivaspin 500 µl centrifugal filter units offer a simple, one step procedure for sample preparation. They can effectively be used in fixed angle rotors accepting 2.2 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations, even with particle laden solutions. Vivaspin 2: for Vivaspin 0.4 to 2 ml The new Vivaspin 2 bridges the gap between the 500 µl and 4 ml centrifugal concentrators. This device combines the speed of the classic Vivaspin products with low internal surface and membrane area for superior recoveries from very dilute solutions. Available with a choice of PES, Cellulose Triacetate and Hydrosart membranes, Vivaspin 2 offers the highest flexibility for process optimisation. Also unique to the Vivaspin 2, is the choice of directly pipetting the concentrate from the dead stop pocket built into the bottom of the concentrator, or alternatively reverse spinning into the concentrate recovery cap which can then be sealed for storage. Both methods result in near total concentrate recoveries. Vivaspin 4: for 1-4 ml samples Vivaspin 4 ml concentrators are disposable ultrafiltration devices for the concentration of biological samples. Maximum initial sample volumes range from 1 ml to 4 ml. They can be effectively used in either swing bucket or fixed angle rotors accepting 15 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimises membrane fouling and provides high speed concentrations, even with particle laden solutions. Vivaspin 4 is available with the high flux polyethersulfone membrane range which is recommended for most solutions. Vivaspin 6: for 2-6 ml samples The Vivaspin 6 centrifugal concentrators have been developed to offer increased volume flexibility and performance. Vivaspin 6 can process 6ml in either swing bucket or fixed angle rotors accepting standard 15ml conical bottom test tubes. High speed and high performance The Vivaspin 6 feature twin vertical membranes for unparalleled filtration speeds and 100X plus concentrations. Remaining volume is easy to read off the printed scale on the side of the concentrator and the modified dead stop pocket further simplifies direct pipette recovery of the final concentrate. Vivaspin 15T: 2-15 ml samples The Vivaspin 15 concentrator is a disposable ultrafiltration device for use in fixed angle or swing bucket rotors accommodating 50 ml tubes. Vivaspin 15 is used for the concentration of biological samples in the 2-15 ml range. The innovative design (US Patent no. 5,647,990, second patent pending), simplicity, speed and exceptional concentrate recoveries are the main features of the concentrator. In a single spin, 15 ml solutions can be concentrated up to 300x. Samples can be typically concentrated in minutes with macromolecular recoveries in excess of 95%. The longitudinal membrane location and adjacent thin channel, provide optimum cross flow conditions even for particle laden solutions, the centrifugal force pulling particles and solids away from the membrane to the bottom of the device. Macromolecules collect in an impermeable 50 µl concentrate pocket integrally moulded below the membrane surface, thereby eliminating the risk of filtration to dryness.

Vivaspin 15R: 2-15 ml samples Vivaspin 15R is the latest member of the Vivaspin product family with all the unique features of Biotech concentrators including a patented vertical membrane and a dead

3 Vivaspin 15R: 2-15 ml samples Vivaspin 15R is the latest member of the Vivaspin product family with all the unique features of Biotech concentrators including a patented vertical membrane and a dead stop. Vivaspin 15R is targeting the volume segment 2 to 15 ml with a modified regenerated cellulose membrane; Hydrosart. This membrane is ideal where extremely high recovery with very low adsorption is needed, for example in applications such as desalting and concentration of Ig fractions. Ultimate recovery at low Adsorption (95-98%) Extremely short concentration time (30x in 15 min.) Convenient application protocol with easy handling Easy scale-up to Vivaflow 200 with Hydrosart membrane for volumes up to 5 litres Very small hold up volume (< 20 µl) Ordering information: Vivaspin Polyethersulfone Membrane Vivaspin 500 Vivaspin 2 Vivaspin 4 Vivaspin 6 Sample min vol. 100µl 0.4ml 1ml 2ml Sample max vol; 600µl 2ml 4ml 6ml MWCO Polyethersulfone Prod. No Prod. No. Prod. No Prod. No. Prod. No Prod. No. Prod. No Prod. No. Membrane (PES) 25/pack 100/pack 25/pack 100/pack 25/pack 100/pack 25/pack 100/pack MWCO PES VS0191 VS0192 VS0291 VS0292 VS0691 VS MWCO PES VS0111 VS0112 VS0211 VS0212 VS0413 VS0212 VS0611 VS MWCO PES VS0101 VS0102 VS0201 VS0202 VS0403 VS0202 VS0601 VS MWCO PES VS0121 VS0122 VS0221 VS0222 VS0423 VS0222 VS0621 VS MWCO PES VS0131 VS0132 VS0231 VS0232 VS0433 VS0232 VS0631 VS MWCO PES VS0141 VS0142 VS0241 VS0242 VS0443 VS0242 VS0641 VS MWCO PES VS0151 VS0152 VS0251 VS VS0651 VS MWCO PES VS0161 VS0162 VS0261 VS VS0661 VS µm VS0171 VS0172 VS0271 VS0272 VS0473 VS0474 VS0671 VS0672 Start pack PES (5 of each 5k, 10k, 30k 50k 100k) VS01S1 - VS02S1 - VS04S3 - VS06S1 - Vivaspin2 (0.4-2 ml samples) Cellulose Triacetate Membrane Prod. No. Prod. No 100/pack 25/pack MWCO CTA VS02U1 VS02U MWCO CTA VS02V1 VS02V MWCO CTA VS02X1 VS02X2 Hydrosart MWCO VS02H91 VS02H MWCO VS02H11 VS02H MWCO VS02H01 VS02H MWCO VS02H21 VS02H22 Vivaspin 15 & 15R Polyethersulfone Membrane (VivaSpin 15 requires 50 ml centrifuge tubes) ; Sample volume:2-15 ml Vivaspin 15T Vivaspin 15R MWCO Polyethersulfone Membrane (PES) Prod. No Prod. No. Prod. No Prod. No. 10/pack 40/pack 12/pack 48/pack MWCO VS15RH91 VS15RH MWCO VS15T11 VS15T12 VS15RH11 VS15RH MWCO VS15T01 VS15Y02 VS15RH01 VS15RH MWCO VS15T21 VS15T22 VS15RH21 VS15RH MWCO VS15T31 VS15T µm VS15T71 VS15T72 - Starter pack (2 of each 5 k, 10 k, 30 k, 50 k, 100 k) VS15S1 - - Accessories Conical bottom 50 ml tubes and lids VSA001 -

4 Performances Time to concentrate up to 30x[min] at 20 C Recovery of protein after concentration [%] Samples: Cytochrome c 0.25 mg/ml (12,400 MW) Insulin chain A 0.1mg/ml (2535 MW) Aprotinin 0.25mg /ml (6500 MW) BSA 1.0 mg / ml ( MW) IgG 0.25 mg / ml ( MW) Latex beads % in DMEM +10 % FCS (0.055 µm) Latex beads % in DMEM +10 % FCS (0.24µm) Yeast 1.0 mg / ml (S. Cerevisiae) Vivaspin 500 Vivaspin 2 Vivaspin 4 Vivaspin 6 Vivaspin 15 Vivaspin 15R Min-max vol. 100 to 600µl 0.4 to 2 ml 1 to 4 ml 2 to 6 ml 2 to 15 ml 2 to 15 ml Centrifugal force x g x g x g x g x g x g x g / x g / g, g, x g x g x g / g, 25 MWCO / Time to Concentrate Time to Concentrate Time to Concentrate Time to Concentrate Time to Concentrate Concentrate Concentrate Membrane type concentr.30x Recovery % concentr.30x Recovery % concentr.30x Recovery % concentr.30x Recovery % concentr.30x Recovery % Recovery % Recovery % Cytochrome c - / - 90 / PES min min / / min / / 94 Insulin chain A 45min PES min min PES min min 70 / / 96 Aprotinin 0.25m / PES 30min 96 50min / / 95 BSA 1.0 mg:ml / PES 15min 96 12min 98 15min / / 98 40min / CTA min min / PES 5min 96 8min 98 10min / / 98 25min / / / CTA min / CTA - - 5min / PES 5min 95 8min 97 10min / 9 98 / 97 25min / / min min IgG 0.25 mg/ml / CTA - - 6min / PES 10min 96 10min 95 10min 95 - / - 96 / 95 30min / / / PES 10min 96 10min 95 10min 95 - / - 96 / 95 30min /PES 10min 96 8min 10min 95 - / - 91 / 91 30min beads 0.055µm /PES / 25 - / Beads 0.24µm /PES / 4 - / Yeast 1.0 mg/ml /PES min µm PES / 3 97 / 97 7min tech +a-chymo, + ova

5 Ordering Information Order No. Order No. Description Pack size Vivaspin 500 High Throughput VS01HT01 Vivaspin 500 HT MWCO PES (includes 120 cap strips) 480 VS01HT21 Vivaspin 500 HT MWCO PES (includes 120 cap strips) 480 Vivaspin 500 Pack Size Prod No. Pack Size Prod No MWCO PES 25 VS VS MWCO PES 25 VS VS MWCO PES 25 VS VS MWCO PES 25 VS VS MWCO PES 25 VS VS MWCO PES 25 VS VS MWCO PES 25 VS VS MWCO PES 25 VS VS µm 25 VS VS0172 Starter pack (5 of each 5 k,10 k,30 k,50 k,100 k) 25 VS01S1 - - VW24HT well Ultrafiltration Frames (includes 2 collection plates) 2 VW24PS well filtrate collection plates 12 Technical Specifications Centrifuge requirements Swing out multiwell plate rotor accepting stacked deep well plates Max RCF with filtrate collection plates Performance Characteristics BSA 1.0 mg/ml Time for concentrate up to 30 x [min.] at g MWCO 15 min 95 % MWCO 15 min 93 % x g Recovery (%) Related products/documents Products HighLights Overview, including: Dialysis systems - selection guide at in our BioSciences Innovations at Information inquire Reply by Fax : +33 (0) or at interbiotech@interchim.com I would like to receive further information on: Title : First name: Surname: Position: Company/Institute: Service, Lab: Adress: Postcode: Town: Tel Fax

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